SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_009140590.1 NCBI__GCF_000225705.1:WP_009140590.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

8gobA Crystal structure of glycerol dehydrogenase in the presence of NAD+ (see paper)
45% identity, 98% coverage: 5:359/363 of query aligns to 3:360/367 of 8gobA

query
sites
8gobA

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binding nicotinamide-adenine-dinucleotide: D37 (≠ S39), F39 (≠ S41), G93 (= G95), G94 (= G96), K95 (≠ S97), D98 (= D100), T116 (= T118), I117 (≠ V119), S119 (≠ A121), T120 (= T122), D121 (= D123), A122 (= A124), S125 (≠ T127), L127 (= L129), V129 (= V131), Y131 (= Y133), A160 (= A162)
binding zinc ion: D171 (= D173), H254 (= H256), H271 (= H272)

8goaA Crystal structure of glycerol dehydrogenase in the absence of NAD+ (see paper)
45% identity, 98% coverage: 5:359/363 of query aligns to 3:360/367 of 8goaA

query
sites
8goaA

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binding zinc ion: D171 (= D173), H254 (= H256), H271 (= H272)

5zxlA Structure of glda from e.Coli (see paper)
45% identity, 98% coverage: 5:359/363 of query aligns to 3:360/367 of 5zxlA

query
sites
5zxlA

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active site: D171 (= D173), H254 (= H256), H257 (≠ Y259), H271 (= H272)
binding glycerol: D121 (= D123), F245 (≠ A247), H254 (= H256)
binding zinc ion: D171 (= D173), H254 (= H256), H271 (= H272)

P0A9S5 Glycerol dehydrogenase; GDH; GLDH; (R)-aminopropanol dehydrogenase; 1,2-propanediol dehydrogenase; D-1-amino-2-propanol oxidoreductase; EC 1.1.1.6; EC 1.1.1.75 from Escherichia coli (strain K12) (see paper)
45% identity, 98% coverage: 5:359/363 of query aligns to 3:360/367 of P0A9S5

query
sites
P0A9S5

R

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F

L

L

L

V

A

A

A

G

G

M

I

G

G

D
|
D

A

A

L

L

G

A

T

T

F

W

F

F

E

E

G

A

R

R

A

A

S

C

I

S

R

R

T

S

E

G

S

A

P

T

S

T

L

M

E

A

G

G

T

G

G

K

I

C

T

T

R

Q

A

A

G

A

M

L

A

A

L

L

A

A

E

E

L

L

C

C

Y

Y

K

N

T

T

L

L

R

L

D

E

Y

E

G

G

S

E

Q

K

A

A

I

M

A

L

A

A

C

A

N

E

A

Q

N

H

V

V

V

V

T

T

P

P

A

A

L

L

D

E

A

R

I

V

I

I

E

E

A

A

N

N

V

T

Y

Y

L

L

S

S

G

G

V

V

G

G

A

F

D

E

N

S

V

G

N

G

C

L

A

A

A

A

A

A

H
|
H

S

A

F

V

Y

H

N

N

G

G

L

L

T

T

A

A

L

I

-

P

G

D

G

A

H

H

S

H

A

Y

P

Y

H
|
H

G

G

C

E

C

K

V

V

A

A

F

F

G

G

T

T

L

L

V

T

Q

Q

L

L

T

V

L

L

E

E

G

N

A

A

T

P

K

V

E

E

E

E

F

I

E

E

D

T

V

V

Q

A

S

A

F

L

C

S

L

H

E

A

V

V

G

G

L

L

P

P

V

I

T

T

L

L

A

A

E

Q

L

L

G

D

V

I

T

K

N

E

N

D

-

V

-

P

D

A

Q

K

V

M

R

R

T

I

I

V

A

A

E

E

N

A

A

A

C

C

V

A

P

E

G

G

E

E

T

T

I

I

H

H

N

N

L

M

A

P

G

G

D

G

V

A

T

T

P

P

D

D

E

Q

L

V

Y

Y

A

A

A

A

I

L

L

L

A

V

T

A

D

D

A

Q

L

Y

G

G

R

Q

D121 (= D123) binding glycerol
D171 (= D173) binding Zn(2+)
H254 (= H256) binding glycerol; binding Zn(2+)
H271 (= H272) binding Zn(2+)

8k1hA Crystal structure of ethylene glycol-bound glycerol dehydrogenase from klebsiella pneumoniae
44% identity, 98% coverage: 5:359/363 of query aligns to 3:360/367 of 8k1hA

query
sites
8k1hA

R

R

A

I

M

I

R

Q

E

S

P

P

L

G

K

K

Y

Y

V

I

Q

Q

G

G

K

T

D

G

A

A

T

I

L

K

Q

R

F

L

H

G

N

D

E

Y

M

L

G

K

Y

P

M

L

G

A

H

E

R

R

W

W

L

L

F

V

I

V

C

G

S
x
D

R

K

S
x
F

G

V

H

L

S

G

A

F

C

A

H

E

D

E

N

M

I

L

E

R

A

K

S

S

F

L

G

A

D

D

S

A

E

G

N

L

Y

A

R

A

R

E

Y

I

E

A

I

P

F

F

G

G

G

G

M

E

S

C

N

S

N

H

G

N

E

E

I

I

A

N

K

R

M

L

Q

R

A

D

I

I

V

A

K

G

D

N

D

A

K

K

I

C

D

T

A

A

V

V

V

L

A

G

V

I

G

G

G
|
G

G
|
G

S
x
K

A

T

V

L

D
|
D

T

T

A

A

K

K

A

A

T

L

A

A

Y

H

Y

F

T

M

G

N

K

V

R

P

V

V

V

A

I

I

I

A

P

P

T
|
T

V
x
I

A

A

A

S

T
|
T

D

D

A
|
A

P

P

C

C

T
x
S

G

A

L
|
L

S

S

V
|
V

I

I

Y
|
Y

N

T

D

D

D

E

G

G

S

E

F

F

D

D

K

S

Y

Y

L

L

F

M

Y

L

P

P

T

R

N

N

P

P

D

N

A

M

V

V

M

I

V

V

D

D

S

T

T

Q

V

I

I

V

A

A

N

G

A

A

P

P

T

A

R

R

F

L

L

L

V

A

A

A

G

G

M

I

G

G

D
|
D

A

A

L

L

G

A

T

T

F

W

F

F

E

E

G

A

R

R

A

A

S

C

I

S

R

R

T

S

E

G

S

A

P

T

S

T

L

M

E

A

G

G

T

G

G

K

I

C

T

T

R

Q

A

A

G

A

M

L

A

A

L

L

A

A

E

E

L

L

C

C

Y

Y

K

N

T

T

L

L

R

L

D

E

Y

E

G

G

S

E

Q

K

A

A

I

M

A

L

A

A

C

A

N

E

A

Q

N

H

V

V

V

V

T

T

P

P

A

A

L

L

D

E

A

R

I

V

I

V

E

E

A

A

N

N

V

T

Y

Y

L

L

S

S

G

G

V

V

G

G

A

F

D

E

N

S

V

G

N

G

C

L

A

A

A

A

A

A

H
|
H

S

A

F

I

Y

H

N

N

G

G

L

M

T

T

A

A

L

I

-

P

G

D

G

A

H

H

S

H

A

Y

P

Y

H
|
H

G

G

C

E

C

K

V

V

A

A

F

F

G

G

T

T

L

L

V

T

Q

Q

L

L

T

V

L

L

E

E

G

N

A

A

T

P

K

V

E

D

E

E

F

I

E

E

D

T

V

V

Q

A

S

A

F

L

C

C

L

H

E

S

V

V

G

G

L

L

P

P

V

I

T

T

L

L

A

A

E

Q

L

L

G

D

V

I

T

K

N

G

N

D

-

I

-

P

D

T

Q

K

V

M

R

R

T

L

I

V

A

A

E

E

N

A

A

A

C

C

V

A

P

E

G

G

E

E

T

T

I

I

H

H

N

N

L

M

A

P

G

G

D

G

V

V

T

D

P

S

D

D

E

Q

L

V

Y

Y

A

A

A

A

I

L

L

L

A

V

T

A

D

D

A

Q

L

Y

G

G

R

Q

binding nicotinamide-adenine-dinucleotide: D37 (≠ S39), F39 (≠ S41), G93 (= G95), G94 (= G96), K95 (≠ S97), D98 (= D100), T116 (= T118), I117 (≠ V119), T120 (= T122), A122 (= A124), S125 (≠ T127), L127 (= L129), V129 (= V131), Y131 (= Y133), H271 (= H272)
binding zinc ion: D171 (= D173), H254 (= H256), H271 (= H272)

8k1gA Crystal structure of ethylene glycol-bound glycerol dehydrogenase from klebsiella pneumoniae
44% identity, 98% coverage: 5:359/363 of query aligns to 3:360/367 of 8k1gA

query
sites
8k1gA

R

R

A

I

M

I

R

Q

E

S

P

P

L

G

K

K

Y

Y

V

I

Q

Q

G

G

K

T

D

G

A

A

T

I

L

K

Q

R

F

L

H

G

N

D

E

Y

M

L

G

K

Y

P

M

L

G

A

H

E

R

R

W

W

L

L

F

V

I

V

C

G

S

D

R

K

S

F

G

V

H

L

S

G

A

F

C

A

H

E

D

E

N

M

I

L

E

R

A

K

S

S

F

L

G

A

D

D

S

A

E

G

N

L

Y

A

R

A

R

E

Y

I

E

A

I

P

F

F

G

G

G

G

M

E

S

C

N

S

N

H

G

N

E

E

I

I

A

N

K

R

M

L

Q

R

A

D

I

I

V

A

K

G

D

N

D

A

K

K

I

C

D

T

A

A

V

V

V

L

A

G

V

I

G

G

G

G

G

G

S

K

A

T

V

L

D

D

T

T

A

A

K

K

A

A

T

L

A

A

Y

H

Y

F

T

M

G

N

K

V

R

P

V

V

V

A

I

I

I

A

P

P

T

T

V

I

A

A

A

S

T

T

D
|
D

A

A

P

P

C

C

T

S

G

A

L

L

S

S

V

V

I

I

Y

Y

N

T

D

D

D

E

G

G

S

E

F

F

D

D

K

S

Y

Y

L

L

F

M

Y

L

P

P

T

R

N

N

P

P

D

N

A

M

V

V

M

I

V

V

D

D

S

T

T

Q

V

I

I

V

A

A

N

G

A

A

P

P

T

A

R

R

F

L

L

L

V

A

A

A

G

G

M

I

G

G

D
|
D

A

A

L

L

G

A

T

T

F

W

F

F

E

E

G

A

R

R

A

A

S

C

I

S

R

R

T

S

E

G

S

A

P

T

S

T

L

M

E

A

G

G

T

G

G

K

I

C

T

T

R

Q

A

A

G

A

M

L

A

A

L

L

A

A

E

E

L

L

C

C

Y

Y

K

N

T

T

L

L

R

L

D

E

Y

E

G

G

S

E

Q

K

A

A

I

M

A

L

A

A

C

A

N

E

A

Q

N

H

V

V

V

V

T

T

P

P

A

A

L

L

D

E

A

R

I

V

I

V

E

E

A

A

N

N

V

T

Y

Y

L

L

S

S

G

G

V

V

G

G

A

F

D

E

N

S

V

G

N

G

C

L

A

A

A

A

A

A

H

H

S

A

F

I

Y

H

N

N

G

G

L

M

T

T

A

A

L

I

-

P

G

D

G

A

H

H

S

H

A

Y

P

Y

H
|
H

G

G

C

E

C

K

V

V

A

A

F

F

G

G

T

T

L

L

V

T

Q

Q

L

L

T

V

L

L

E

E

G

N

A

A

T

P

K

V

E

D

E

E

F

I

E

E

D

T

V

V

Q

A

S

A

F

L

C

C

L

H

E

S

V

V

G

G

L

L

P

P

V

I

T

T

L

L

A

A

E

Q

L

L

G

D

V

I

T

K

N

G

N

D

-

I

-

P

D

T

Q

K

V

M

R

R

T

L

I

V

A

A

E

E

N

A

A

A

C

C

V

A

P

E

G

G

E

E

T

T

I

I

H

H

N

N

L

M

A

P

G

G

D

G

V

V

T

D

P

S

D

D

E

Q

L

V

Y

Y

A

A

A

A

I

L

L

L

A

V

T

A

D

D

A

Q

L

Y

G

G

R

Q

binding zinc ion: D121 (= D123), D171 (= D173), H271 (= H272)

5xn8A Structure of glycerol dehydrogenase crystallised as a contaminant
44% identity, 99% coverage: 3:360/363 of query aligns to 1:361/365 of 5xn8A

query
sites
5xn8A

L

M

L

L

R

K

A

V

M

I

R

Q

E

S

P

P

L

G

K

K

Y

Y

V

L

Q

Q

G

G

K

P

D

D

A

A

T

S

L

T

Q

L

F

F

H

G

N

A

E

Y

M

A

G

K

Y

N

M

L

G

A

H

E

R

S

W

F

L

F

F

V

I

I

C

A

S

D

R

D

S

F

G

V

H

M

S

K

A

L

C

A

H

G

D

D

N

K

I

V

E

L

A

N

S

G

F

L

G

H

D

S

S

H

E

D

N

I

Y

S

R

L

R

H

Y

A

E

E

I

R

F

F

G

N

G

G

M

E

S

C

N

S

N

H

G

V

E

E

I

I

A

R

K

R

M

L

Q

I

A

A

I

I

V

L

K

K

D

Q

D

H

K

G

I

C

D

R

A

G

V

V

V

V

A

G

V

V

G

G

G

G

G

G

S

K

A

T

V

L

D

D

T

T

A

A

K

K

A

A

T

I

A

G

Y

Y

Y

Y

T

Q

G

K

K

L

R

P

V

V

V

V

I

V

I

I

P

P

T

T

V

I

A

A

A

S

T

T

D

D

A

A

P

P

C

T

T

S

G

A

L

L

S

S

V

V

I

I

Y

Y

N

T

D

E

D

A

G

G

S

E

F

F

D

E

K

E

Y

Y

L

L

F

I

Y

Y

P

P

T

K

N

N

P

P

D

D

A

M

V

V

M

V

V

M

D

D

S

T

T

A

V

I

I

I

A

A

N

K

A

A

P

P

T

V

R

R

F

L

L

L

V

V

A

S

G

G

M

M

G

G

D
|
D

A

A

L

L

G

S

T

T

F

W

F

F

E

E

G

A

R

K

A

A

S

C

I

Y

R

D

T

A

E

R

S

A

P

T

S

S

L

M

E

A

G

G

T

G

G

Q

I

S

T

T

R

E

A

A

G

A

M

L

A

S

L

L

A

A

E

R

L

L

C

C

Y

Y

K

D

T

T

L

L

R

L

D

A

Y

E

G

G

S

E

Q

K

A

A

I

R

A

L

A

A

C

A

N

Q

A

A

N

G

V

V

V

V

T

T

P

D

A

A

L

L

D

E

A

R

I

I

I

I

E

E

A

A

N

N

V

T

Y

Y

L

L

S

S

G

G

V

I

G

G

A

F

D

E

N

S

V

S

N

G

C

L

A

A

A

A

A

A

H
|
H

S

A

F

I

Y

H

N

N

G

G

L

F

T

T

A

I

L

L

G

E

G

E

-

C

H

H

S

H

A

L

P

Y

H
|
H

G

G

C

E

C

K

V

V

A

A

F

F

G

G

T

T

L

L

V

A

Q

Q

L

L

T

V

L

L

E

Q

G

N

A

S

T

P

K

M

E

E

E

E

F

I

E

E

D

T

V

V

Q

L

S

G

F

F

C

C

L

E

E

K

V

V

G

G

L

L

P

P

V

V

T

T

L

L

A

A

E

Q

L

M

G

G

V

V

T

K

N

E

-

G

-

I

N

D

D

D

Q

K

V

I

R

A

T

A

I

V

A

A

E

K

N

A

A

T

C

C

V

A

P

E

G

G

E

E

T

T

I

I

H

H

N

N

L

M

A

P

G

F

D

A

V

V

T

T

P

P

D

E

E

Q

L

V

Y

H

A

A

A

A

I

I

L

L

A

T

T

A

D

D

A

L

L

L

G

G

R

Q

S

Q

binding zinc ion: D171 (= D173), H254 (= H256), H271 (= H272)

1kq3A Crystal structure of a glycerol dehydrogenase (tm0423) from thermotoga maritima at 1.5 a resolution (see paper)
44% identity, 96% coverage: 10:359/363 of query aligns to 9:359/364 of 1kq3A

query
sites
1kq3A

P

P

L

G

K

R

Y

Y

V

V

Q

Q

G

G

K

A

D

G

A

A

T

I

L

N

Q

I

F

L

H

E

N

E

E

E

M

L

G

S

Y

R

M

F

G

G

H

E

R

R

-

A

W

F

L

V

F

V

I

I

C

D

S

D

R

F

S

V

G

D

H

K

S

N

A

V

C

L

H

G

D

E

N

N

I

F

E

F

A

S

S

S

F

F

G

T

D

K

S

V

E

R

N

V

Y

N

R

K

R

-

Y

-

E

Q

I

I

F

F

G

G

G

G

M

E

S

C

N

S

N

D

G

E

E

E

I

I

A

E

K

R

M

L

Q

S

A

G

I

L

V

V

K

E

D

E

D

E

K

-

I

T

D

D

A

V

V

V

V

V

A

G

V

I

G

G

G

G

G

G

S

K

A

T

V

L

D

D

T

T

A

A

K

K

A

A

T

V

A

A

Y

Y

Y

K

T

L

G

K

K

K

R

P

V

V

V

V

I

I

I

V

P

P

T

T

V

I

A

A

A

S

T

T

D

D

A

A

P

P

C

C

T

S

G

A

L

L

S

S

V

V

I

I

Y

Y

N

T

D

P

D

N

G

G

S

E

F

F

D

K

K

R

Y

Y

L

L

F

F

Y

L

P

P

T

R

N

N

P

P

D

D

A

V

V

V

M

L

V

V

D

D

S

T

T

E

V

I

I

V

A

A

N

K

A

A

P

P

T

A

R

R

F

F

L

L

V

V

A

A

G

G

M

M

G

G

D
|
D

A

A

L

L

G

A

T

T

F

W

F

F

E

E

G

A

R

E

A

S

S

C

I

K

R

Q

T

K

E

Y

S

A

P

P

S

N

L

M

E

T

G

G

T

R

G

L

I

G

T

S

R

M

A

T

G

A

M

Y

A

A

L

L

A

A

E

R

L

L

C

C

Y

Y

K

E

T

T

L

L

R

L

D

E

Y

Y

G

G

S

V

Q

L

A

A

I

K

A

R

A

S

C

V

N

E

A

E

N

K

V

S

V

V

T

T

P

P

A

A

L

L

D

E

A

K

I

I

I

V

E

E

A

A

N

N

V

T

Y

L

L

L

S

S

G

G

V

L

G

G

A

F

D

E

N

S

V

G

N

G

C

L

A

A

A

A

A

A

H
|
H

S

A

F

I

Y
x
H

N

N

G

G

L

L

T

T

A

V

L

L

-

E

G

N

G

T

H

H

S

K

A

Y

P

L

H
|
H

G

G

C

E

C

K

V

V

A

A

F

I

G

G

T

V

L

L

V

A

Q

S

L

L

T

F

L

L

E

T

G

D

A

K

T

P

K

R

E

K

E

M

F

I

E

E

D

E

V

V

Q

Y

S

S

F

F

C

C

L

E

E

E

V

V

G

G

L

L

P

P

V

T

T

T

L

L

A

A

E

E

L

I

G

G

V

L

-

D

-

G

T

V

N

S

N

D

D

E

Q

D

V

L

R

M

T

K

I

V

A

A

E

E

N

K

A

A

C

C

V

D

P

K

G

N

E

E

T

T

I

I

H

H

N

N

L

E

A

P

G

Q

D

P

V

V

T

T

P

S

D

K

E

D

L

V

Y

F

A

F

A

A

I

L

L

K

A

A

T

A

D

D

A

R

L

Y

G

G

R

R

active site: D170 (= D173), H253 (= H256), H256 (≠ Y259), H270 (= H272)
binding zinc ion: D170 (= D173), H253 (= H256), H270 (= H272)

Q9WYQ4 Glycerol dehydrogenase; GDH; GLDH; EC 1.1.1.6 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
44% identity, 96% coverage: 10:359/363 of query aligns to 8:358/364 of Q9WYQ4

query
sites
Q9WYQ4

P

P

L

G

K

R

Y

Y

V

V

Q

Q

G

G

K

A

D

G

A

A

T

I

L

N

Q

I

F

L

H

E

N

E

E

E

M

L

G

S

Y

R

M

F

G

G

H

E

R

R

-

A

W

F

L

V

F

V

I

I

C

D

S

D

R

F

S

V

G

D

H

K

S

N

A

V

C

L

H

G

D

E

N

N

I

F

E

F

A

S

S

S

F

F

G

T

D

K

S

V

E

R

N

V

Y

N

R

K

R

-

Y

-

E

Q

I

I

F

F

G

G

G

G

M

E

S

C

N

S

N

D

G

E

E

E

I

I

A

E

K

R

M

L

Q

S

A

G

I

L

V

V

K

E

D

E

D

E

K

-

I

T

D

D

A

V

V

V

V

V

A

G

V

I

G

G

G

G

G

G

S

K

A

T

V

L

D

D

T

T

A

A

K

K

A

A

T

V

A

A

Y

Y

Y

K

T

L

G

K

K

K

R

P

V

V

V

V

I

I

I

V

P

P

T

T

V

I

A

A

A

S

T

T

D

D

A

A

P

P

C

C

T

S

G

A

L

L

S

S

V

V

I

I

Y

Y

N

T

D

P

D

N

G

G

S

E

F

F

D

K

K

R

Y

Y

L

L

F

F

Y

L

P

P

T

R

N

N

P

P

D

D

A

V

V

V

M

L

V

V

D

D

S

T

T

E

V

I

I

V

A

A

N

K

A

A

P

P

T

A

R

R

F

F

L

L

V

V

A

A

G

G

M

M

G

G

D
|
D

A

A

L

L

G

A

T

T

F

W

F

F

E

E

G

A

R

E

A

S

S

C

I

K

R

Q

T

K

E

Y

S

A

P

P

S

N

L

M

E

T

G

G

T

R

G

L

I

G

T

S

R

M

A

T

G

A

M

Y

A

A

L

L

A

A

E

R

L

L

C

C

Y

Y

K

E

T

T

L

L

R

L

D

E

Y

Y

G

G

S

V

Q

L

A

A

I

K

A

R

A

S

C

V

N

E

A

E

N

K

V

S

V

V

T

T

P

P

A

A

L

L

D

E

A

K

I

I

I

V

E

E

A

A

N

N

V

T

Y

L

L

L

S

S

G

G

V

L

G

G

A

F

D

E

N

S

V

G

N

G

C

L

A

A

A

A

A

A

H
|
H

S

A

F

I

Y

H

N

N

G

G

L

L

T

T

A

V

L

L

-

E

G

N

G

T

H

H

S

K

A

Y

P

L

H
|
H

G

G

C

E

C

K

V

V

A

A

F

I

G

G

T

V

L

L

V

A

Q

S

L

L

T

F

L

L

E

T

G

D

A

K

T

P

K

R

E

K

E

M

F

I

E

E

D

E

V

V

Q

Y

S

S

F

F

C

C

L

E

E

E

V

V

G

G

L

L

P

P

V

T

T

T

L

L

A

A

E

E

L

I

G

G

V

L

-

D

-

G

T

V

N

S

N

D

D

E

Q

D

V

L

R

M

T

K

I

V

A

A

E

E

N

K

A

A

C

C

V

D

P

K

G

N

E

E

T

T

I

I

H

H

N

N

L

E

A

P

G

Q

D

P

V

V

T

T

P

S

D

K

E

D

L

V

Y

F

A

F

A

A

I

L

L

K

A

A

T

A

D

D

A

R

L

Y

G

G

R

R

D169 (= D173) binding Zn(2+)
H252 (= H256) binding Zn(2+)
H269 (= H272) binding Zn(2+)

4mcaA Crystal structure of glycerol dehydrogenase from serratia to 1.9a
42% identity, 99% coverage: 3:360/363 of query aligns to 1:361/367 of 4mcaA

query
sites
4mcaA

L

M

L

L

R

R

A

I

M

I

R

Q

E

S

P

P

L

G

K

K

Y

Y

V

I

Q

Q

G

G

K

A

D

N

A

A

T

L

L

A

Q

A

F

V

H

G

N

Q

E

Y

M

A

G

K

Y

S

M

L

G

A

H

D

R
x
H

W

Y

L

L

F

V

I

I

C

A

S

D

R

D

S

F

G

V

H

M

S

K

A

L

C

A

H

G

D

D

N

T

I

L

E

M

A

G

S

S

F

L

G

Q

D

Q

S

H

E

G

N

V

Y

K

R

H

R

H

Y

A

E

A

I

L

F

F

G

N

G

G

M

E

S

C

N

C

N

H

G

K

E

E

I

I

A

D

K

R

M

L

Q

G

A

R

I

E

V

L

K

K

D

A

D

H

K

G

I
x
C

D

R

A

G

V

V

V

I

A

G

V

V

G

G

G

G

G

G

S

K

A

T

V

L

D

D

T

T

A

A

K

K

A

A

T

I

A

A

Y

H

Y

Y

T

Q

G

Q

K

L

R

P

V

V

V

V

I

L

I

I

P

P

T

T

V

I

A

A

A

S

T

T

D

D

A

A

P

P

C

T

T

S

G

A

L

L

S

S

V

V

I

I

Y

Y

N

T

D

E

D

Q

G

G

S

E

F

F

D

A

K

E

Y

Y

L

L

F

I

Y

Y

P

P

T

R

N

N

P

P

D

D

A

M

V

V

M

V

V

M

D

D

S

V

T

A

V

I

I

I

A

A

N

K

A

A

P

P

T

V

R

R

F

L

L

L

V

V

A

A

G

G

M

M

G

G

D
|
D

A

A

L

L

G

S

T

T

F

Y

F

F

E

E

G

A

R

Q

A

A

S

C

I

F

R

D

T

A

E

Q

S

A

P

T

S

S

L

M

E

A

G

G

T

G

G

K

I

S

T

T

R

L

A

A

G

A

M

L

A

S

L

L

A

A

E

R

L

L

C

C

Y

Y

K

D

T

T

L

L

R

L

D

A

Y

E

G

G

S

V

Q

K

A

A

I

K

A

L

A

A

C

V

N

E

A

A

N

G

V

V

V

V

T

T

P

E

A

A

L

V

D

E

A

R

I

I

I

I

E

E

A

A

N

N

V

T

Y

Y

L

L

S

S

G

G

V

I

G

G

A

F

D

E

N

S

V

S

N

G

C

L

A

A

A

A

A

A

H
|
H

S

A

F

I

Y

H

N

N

G

G

L

F

T

T

A

V

L

L

G

E

G

E

-

C

H

H

S

H

A

L

P

Y

H
|
H

G

G

C

E

C

K

V

V

A

A

F

F

G

G

T

T

L

L

V

A

Q

Q

L

L

T

V

L

L

E

Q

G

N

A

S

T

P

K

M

E

A

E

Q

F

I

E

E

D

T

V

V

Q

L

S

A

F

F

C

C

L

H

E

R

V

I

G

G

L

L

P

P

V

I

T

T

L

L

A

A

E

E

L

M

G

G

V

V

T

S

N

G

N

D

-

A

-

V

D

E

Q

K

V

I

R

M

T

A

I

V

A

A

E

Q

N

A

A

S

C

C

V

A

P

A

G

G

E

E

T

T

I

I

H

H

N

N

L

M

A

P

G

F

D

K

V

V

T

T

P

P

D

A

E

G

L

V

Y

Q

A

A

A

A

I

I

L

L

A

T

T

A

D

D

A

R

L

L

G

G

R

S

S

A

binding zinc ion: H31 (≠ R33), C85 (≠ I87), D171 (= D173), H254 (= H256), H271 (= H272)

P32816 Glycerol dehydrogenase; GDH; GLDH; GlyDH; EC 1.1.1.6 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see 2 papers)
40% identity, 99% coverage: 1:360/363 of query aligns to 1:363/370 of P32816

query
sites
P32816

M

M

K

A

L

A

L

E

R

R

A

V

M

F

R

I

E

S

P

P

L

A

K

K

Y

Y

V

V

Q

Q

G

G

K

K

D

N

A

V

T

I

L

T

Q

K

F

I

H

A

N

N

E

Y

M

L

G

E

Y

G

M

I

G

G

H

N

R

K

W

T

L

V

F

V

I

I

C

A

S
x
D

R

E

-

I

-

V

-

W

-

K

-

I

S

A

G

G

H

H

S

T

A

I

C

V

H

N

D

E

N

L

I

K

E

K

A

G

S

N

F

I

G

A

D

A

S

E

E

E

N

V

Y

-

R

-

R

-

Y

-

E

-

I

V

F

F

G

S

G

G

M

E

S

A

N

S

N

R

G

N

E

E

I

V

A

E

K

R

M

I

Q

A

A

N

I

I

V

A

K

R

D

K

D

A

K

E

I

A

D

A

A

I

V

V

V

I

A

G

V

V

G

G

G

G

G
|
G

S
x
K

A

T

V

L

D

D

T

T

A

A

K

K

A

A

T

V

A

A

Y

D

Y

E

T

L

G

D

K

A

R

Y

V

I

V

V

I

I

I

V

P

P

T
|
T

V

A

A

A

A
x
S

T

T

D
|
D

A

A

P

P

C

T

T
x
S

G

A

L
|
L

S

S

V

V

I

I

Y
|
Y

N

S

D

D

D

D

G

G

S

V

F

F

D

E

K

S

Y

Y

L

R

F

F

Y

Y

P

K

T

K

N

N

P

P

D

D

A

L

V

V

M

L

V

V

D

D

S

T

T

K

V

I

I

I

A

A

N

N

A

A

P

P

T

P

R

R

F

L

L

L

V

A

A

S

G

G

M

I

G

A

D
|
D

A

A

L

L

G

A

T

T

F

W

F

V

E

E

G

A

R

R

A

S

S

V

I

I

R

K

T

S

E

G

S

G

P

K

S

T

L

M

E

A

G

G

T

G

G

I

I

P

T

T

R

I

A

A

G

A

M

E

A

A

L

I

A

A

E

E

L

K

C

C

Y

E

K

Q

T

T

L

L

R

F

D

K

Y

Y

G

G

S

K

Q

L

A

A

I

Y

A

E

A

S

C

V

N

K

A

A

N

K

V

V

V

V

T

T

P

P

A

A

L

L

D

E

A

A

I

V

I

V

E

E

A

A

N

N

V

T

Y

L

L

L

S

S

G

G

V

L

G

G

A

F

D

E

N

S

V

G

N

G

C

L

A

A

A

A

A

A

H
|
H

S

A

F

I

Y

H

N

N

G

G

L

F

T

T

A

A

L

L

G

E

G

G

-

E

-

I

H

H

S

H

A

L

P

T

H
|
H

G

G

C

E

C

K

V

V

A

A

F

F

G

G

T

T

L

L

V

V

Q

Q

L

L

T

A

L

L

E

E

G

E

A

H

T

S

K

Q

E

Q

E

E

F

I

E

E

D

R

V

Y

Q

I

S

E

F

L

C

Y

L

L

E
x
S

V

L

G

D

L

L

P

P

V

V

T

T

L

L

A

E

E

D

L

I

G

K

V

L

T

K

N

D

-

A

-

S

N

R

D

E

Q

D

V

I

R

L

T

K

I

V

A

A

E

K

N

A

A

A

C

T

V

A

P

E

G

G

E

E

T

T

I

I

H

H

N

N

L

-

A

A

G

F

D

N

V

V

T

T

P

A

D

D

E

D

L

V

Y

A

A

D

A

A

I

I

L

F

A

A

T

A

D

D

A

Q

L

Y

G

A

R

K

S

A

D39 (≠ S39) binding NAD(+)
G96 (= G96) binding NAD(+)
K97 (≠ S97) binding NAD(+); mutation to H: Loss of activity.
T118 (= T118) binding NAD(+)
S121 (≠ A121) binding NAD(+)
D123 (= D123) binding glycerol
S127 (≠ T127) binding NAD(+)
L129 (= L129) binding NAD(+)
Y133 (= Y133) binding NAD(+)
D173 (= D173) binding glycerol; binding Zn(2+)
H256 (= H256) binding glycerol; binding Zn(2+)
H274 (= H272) binding Zn(2+)
S305 (≠ E303) mutation to C: No effect on affinity for substrates.

1jq5A Bacillus stearothermophilus glycerol dehydrogenase complex with NAD+ (see paper)
40% identity, 98% coverage: 5:360/363 of query aligns to 4:362/366 of 1jq5A

query
sites
1jq5A

R

R

A

V

M

F

R

I

E

S

P

P

L

A

K

K

Y

Y

V

V

Q

Q

G

G

K

K

D

N

A

V

T

I

L

T

Q

K

F

I

H

A

N

N

E

Y

M

L

G

E

Y

G

M

I

G

G

H

N

R

K

W

T

L

V

F

V

I

I

C

A

S
x
D

R

E

-

I

-

V

-

W

-

K

-
x
I

S

A

G

G

H

H

S

T

A

I

C

V

H

N

D

E

N

L

I

K

E

K

A

G

S

N

F

I

G

A

D

A

S

E

E

E

N

V

Y

-

R

-

R

-

Y

-

E

-

I

V

F

F

G

S

G

G

M

E

S

A

N

S

N

R

G

N

E

E

I

V

A

E

K

R

M

I

Q

A

A

N

I

I

V

A

K

R

D

K

D

A

K

E

I

A

D

A

A

I

V

V

V

I

A

G

V

V

G

G

G
|
G

G
|
G

S
x
K

A

T

V

L

D
|
D

T

T

A

A

K

K

A

A

T

V

A

A

Y

D

Y

E

T

L

G

D

K

A

R

Y

V

I

V

V

I

I

I

V

P

P

T
|
T

V

A

A

A

A
x
S

T
|
T

D
|
D

A
|
A

P

P

C

T

T
x
S

G

A

L
|
L

S

S

V
|
V

I

I

Y
|
Y

N

S

D

D

D

D

G

G

S

V

F

F

D

E

K

S

Y

Y

L

R

F

F

Y

Y

P

K

T

K

N

N

P

P

D

D

A

L

V

V

M

L

V

V

D

D

S

T

T

K

V

I

I

I

A

A

N

N

A
|
A

P
|
P

T

P

R

R

F

L

L

L

V

A

A

S

G

G

M

I

G

A

D
|
D

A

A

L

L

G

A

T

T

F

W

F

V

E

E

G

A

R

R

A

S

S

V

I

I

R

K

T

S

E

G

S

G

P

K

S

T

L

M

E

A

G

G

T

G

G

I

I

P

T

T

R

I

A

A

G

A

M

E

A

A

L

I

A

A

E

E

L

K

C

C

Y

E

K

Q

T

T

L

L

R

F

D

K

Y

Y

G

G

S

K

Q

L

A

A

I

Y

A

E

A

S

C

V

N

K

A

A

N

K

V

V

V

V

T

T

P

P

A

A

L

L

D

E

A

A

I

V

I

V

E

E

A

A

N

N

V

T

Y

L

L

L

S

S

G

G

V

L

G

G

A

F

D

E

N

S

V

G

N

G

C

L

A

A

A

A

A

A

H
|
H

S

A

F

I

Y
x
H

N

N

G

G

L

F

T

T

A

A

L

L

G

E

G

G

-
x
E

-

I

H

H

S
x
H

A

L

P

T

H
|
H

G

G

C

E

C

K

V

V

A

A

F

F

G

G

T

T

L

L

V

V

Q

Q

L

L

T

A

L

L

E

E

G

E

A

H

T

S

K

Q

E

Q

E

E

F

I

E

E

D

R

V

Y

Q

I

S

E

F

L

C

Y

L

L

E

C

V

L

G

D

L

L

P

P

V

V

T

T

L

L

A

E

E

D

L

I

G

K

V

L

T

K

N

D

-

A

-

S

N

R

D

E

Q

D

V

I

R

L

T

K

I

V

A

A

E

K

N

A

A

A

C

T

V

A

P

E

G

G

E

E

T

T

I

I

H

H

N

N

L

-

A

A

G

F

D

N

V

V

T

T

P

A

D

D

E

D

L

V

Y

A

A

D

A

A

I

I

L

F

A

A

T

A

D

D

A

Q

L

Y

G

A

R

K

S

A

active site: D172 (= D173), H255 (= H256), H258 (≠ Y259), H273 (= H272)
binding nicotinamide-adenine-dinucleotide: D38 (≠ S39), I44 (vs. gap), G94 (= G95), G95 (= G96), K96 (≠ S97), D99 (= D100), T117 (= T118), S120 (≠ A121), T121 (= T122), D122 (= D123), A123 (= A124), S126 (≠ T127), L128 (= L129), V130 (= V131), Y132 (= Y133), A161 (= A162), P162 (= P163), H273 (= H272)
binding zinc ion: D172 (= D173), H255 (= H256), E267 (vs. gap), H270 (≠ S269), H273 (= H272)

3uhjA Crystal structure of a probable glycerol dehydrogenase from sinorhizobium meliloti 1021
41% identity, 99% coverage: 3:361/363 of query aligns to 1:357/357 of 3uhjA

query
sites
3uhjA

L

M

L

A

R

R

A

A

M

F

R

G

E

G

P

P

L

N

K

K

Y

Y

V

I

Q

Q

G

R

K

A

D

G

A

E

T

I

L

D

Q

K

F

L

H

A

N

A

E

Y

M

L

G

A

Y

P

M

L

G

G

H

K

R

R

W

A

L

L

F

V

I

L

C

I

S

D

R

R

S

V

G

L

H

F

S

D

A

A

C

L

H

S

D

E

N

R

I

I

E

S

A

L

S

D

F

I

G

-

D

-

S

-

E

-

N

-

Y

-

R

-

R

R

Y

F

E

E

I

R

F

F

G

G

G

G

M

E

S

C

N

C

N

T

G

S

E

E

I

I

A

E

K

R

M

V

Q

R

A

K

I

V

V

A

K

I

D

E

D

H

K

G

I

S

D

D

A

I

V

L

V

V

A

G

V

V

G

G

G

G

G

G

S

K

A

T

V

A

D

D

T

T

A

A

K

K

A

I

T

V

A

A

Y

I

Y

D

T

T

G

G

K

A

R

R

V

I

V

V

I

I

I

A

P

P

T

T

V

I

A

A

A

S

T

T

D

D

A

A

P

P

C

C

T

S

G

A

L

I

S

A

V

V

I

R

Y

Y

N

T

D

E

D

H

G

G

S

V

F

Y

D

E

K

E

Y

A

L

L

F

R

Y

L

P

P

T

R

N

N

P

P

D

D

A

A

V

V

M

V

V

V

D

D

S

S

T

A

V

L

I

V

A

A

N

A

A

A

P

P

T

A

R

R

F

F

L

L

V

V

A

A

G

G

M

I

G

G

D
|
D

A

A

L

L

G

S

T

T

F

W

F

F

E

E

G

A

R

R

A

S

S

N

I

I

-

E

-

S

R

R

T

T

E

D

S

N

P

Y

S

V

L

A

E

G

G

G

T

F

G

P

I

A

T

T

R

E

A

A

G

G

M

M

A

A

L

I

A

A

E

R

L

H

C

C

Y

Q

K

D

T

V

L

L

R

T

D

R

Y

D

G

A

S

V

Q

K

A

A

I

K

A

I

A

A

C

V

N

E

A

A

N

G

V

L

V

L

T

T

P

P

A

A

L

V

D

E

A

N

I

I

I

I

E

E

A

A

N

N

V

T

Y

L

L

L

S

S

G

G

V

L

G

G

A

F

D

E

N

N

V

C

N

G

C

C

A

S

A

A</