SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_009152150.1 NCBI__GCF_000244955.1:WP_009152150.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q5SKN9 Long-chain-fatty-acid--CoA ligase; Long-chain fatty acyl-CoA synthetase; LC-FACS; EC 6.2.1.3 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
41% identity, 95% coverage: 8:508/525 of query aligns to 16:533/541 of Q5SKN9

query
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Q5SKN9

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T184 (= T165) binding Mg(2+)
G302 (= G278) binding tetradecanoyl-AMP
Q322 (≠ H298) binding tetradecanoyl-AMP
G323 (≠ L299) binding tetradecanoyl-AMP
T327 (= T303) binding tetradecanoyl-AMP
E328 (= E304) binding Mg(2+)
D418 (= D394) binding tetradecanoyl-AMP
K435 (= K411) binding tetradecanoyl-AMP
K439 (≠ I415) binding tetradecanoyl-AMP

1v26B Crystal structure of tt0168 from thermus thermophilus hb8 (see paper)
39% identity, 93% coverage: 8:495/525 of query aligns to 9:496/510 of 1v26B

query
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active site: T177 (= T165), H197 (≠ Q185), H223 (= H209), T320 (= T303), E321 (= E304), K432 (≠ I415), W437 (≠ N420)
binding adenosine monophosphate: G295 (= G278), S296 (≠ A279), A297 (≠ P280), G316 (≠ L299), Y317 (= Y300), G318 (= G301), L319 (= L302), T320 (= T303), D411 (= D394), K428 (= K411), K432 (≠ I415), W437 (≠ N420)
binding magnesium ion: T177 (= T165), E321 (= E304)

1v25A Crystal structure of tt0168 from thermus thermophilus hb8 (see paper)
40% identity, 86% coverage: 8:457/525 of query aligns to 9:464/491 of 1v25A

query
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1v25A

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active site: T177 (= T165), H197 (≠ Q185), H223 (= H209), T320 (= T303), E321 (= E304), K432 (≠ I415), W437 (≠ N420)
binding phosphoaminophosphonic acid-adenylate ester: H223 (= H209), V224 (≠ C210), G295 (= G278), S296 (≠ A279), A297 (≠ P280), Y317 (= Y300), G318 (= G301), L319 (= L302), T320 (= T303), D411 (= D394), I423 (≠ L406), K432 (≠ I415), W437 (≠ N420)
binding magnesium ion: T177 (= T165), E321 (= E304)

P0DX84 3-methylmercaptopropionyl-CoA ligase; MMPA-CoA ligase; EC 6.2.1.44 from Ruegeria lacuscaerulensis (strain DSM 11314 / KCTC 2953 / ITI-1157) (Silicibacter lacuscaerulensis) (see paper)
34% identity, 97% coverage: 1:507/525 of query aligns to 1:531/539 of P0DX84

query
sites
P0DX84

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H231 (= H209) mutation to A: Retains 74% of wild-type activity.
W235 (= W213) mutation to A: Almost completely abolishes the activity.
G302 (= G277) mutation to P: Almost completely abolishes the activity.
G303 (= G278) mutation to P: Almost completely abolishes the activity.
W326 (≠ Y300) mutation to A: Retains 7.7% of wild-type activity.
P333 (vs. gap) mutation to A: Retains 69% of wild-type activity.
R432 (= R409) mutation to A: Retains 4.3% of wild-type activity.
K434 (= K411) mutation to A: Retains 36% of wild-type activity.
D435 (= D412) mutation to A: Retains 76% of wild-type activity.
K438 (≠ I415) mutation to A: Retains 5.6% of wild-type activity.
G440 (= G417) mutation to P: Retains 3.6% of wild-type activity.
G441 (= G418) mutation to P: Retains 2.7% of wild-type activity.
E442 (= E419) mutation to A: Retains 27% of wild-type activity.
W443 (≠ N420) mutation to A: Retains 60% of wild-type activity.
E474 (= E451) mutation to A: Retains 33% of wild-type activity.
K523 (= K499) Plays an important role in catalysis; mutation to A: Retains 1.6% of wild-type activity.; mutation to E: Retains 1.4% of wild-type activity.; mutation to R: Retains 57% of wild-type activity.
K526 (= K502) mutation to A: Retains 48% of wild-type activity.

6ijbB Structure of 3-methylmercaptopropionate coa ligase mutant k523a in complex with amp and mmpa (see paper)
34% identity, 97% coverage: 1:507/525 of query aligns to 1:531/538 of 6ijbB

query
sites
6ijbB

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Q

N

A

I

I

A

A

V

E

A

H

H

P

P

A

K

V

L

F

A

E

T

V

A

A

A

V

V

I

I

A

G

V

V

P

P

D

H

D

P

H

K

W

W

G

D

E

E

V

R

P

P

A

L

Y

V

V

A

T

V

V

K

H

A

E

E

G

G

A

E

S

D

V

P

S

S

E

E

S

A

E

E

L

L

I

L

A

E

H

F

V

F

R

D

E

G

R

K

L

I

A

A

H

K

F

W

K

Q

A

V

P

P

K

D

S

K

V

V

F

F

G

V

E

D

-

A

L

L

P

P

K

L

T

N

S

A

T

T

G

G

K
x
A

I

V

Q

L

K

K

Y

R

V

K

L

L

R

R

E

D

active site: T185 (= T165), H205 (≠ Q185), H231 (= H209), S329 (≠ T303), E330 (= E304), K438 (≠ I415), W443 (≠ N420), A523 (≠ K499)
binding 3-(methylsulfanyl)propanoic acid: W235 (= W213), G303 (= G278), A325 (≠ L299), W326 (≠ Y300), G327 (= G301), M328 (≠ L302)
binding adenosine monophosphate: G303 (= G278), A304 (= A279), A305 (≠ P280), H324 (= H298), W326 (≠ Y300), G327 (= G301), M328 (≠ L302), S329 (≠ T303), Q359 (= Q333), D417 (= D394)

6ihkB Structure of mmpa coa ligase in complex with adp (see paper)
33% identity, 97% coverage: 1:507/525 of query aligns to 1:528/533 of 6ihkB

query
sites
6ihkB

M

M

T

L

G

G

D

Q

L

M

S

M

F

T

E

Q

P

P

L

L

T

L

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I

V

S

S

S

Y

L

L

I

D

D

R

H

A

A

A

R

A

Y

H

H

G

G

S

-

R

Q

T

T

A

E

V

I

V

V

D

S

G

V

E

E

H

T

R

D

W

G

T

T

Y

V

T

T

-

R

-

T

-

N

-

W

-

G

Q

E

L

I

H

A

D

A

R

N

C

A

R

R

L

M

A

G

G

S

G

A

L

L

A

T

A

K

-

L

-

G

-

L

P

Q

A

P

E

Q

G

D

R

R

P

-

V

I

A

G

V

T

L

L

A

A

A

W

N

N

T

N

H

R

V

R

L

H

L

L

E

E

A

I

N

Y

F

Y

G

A

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A

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W

G

A

A

G

G

V

F

P

V

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C

V

H

A

T

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I

N

N

T

P

R

R

L

L

S

F

A

P

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E

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A

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Y

I

I

L

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E

N

H

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C

A

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A

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L

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F

H

F

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A

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L

L

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V

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A

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A

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E

E

P

V

P

K

V

H

V

F

I

V

R

L

A

M

G

G

-

P

-

R

-

N

-

E

-

D

-

A

-

L

-

Q

-

I

-

P

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G

-

L

E

E

E

F

Y

Y

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Q

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A

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A

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P

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M

D

A

F

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-

F

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D

E

E

R

N

A

T

L

A

L

S

S

S

I

L

N

C

Y

Y

T
|
T

S

S

G

G

T

-

G

-

R

H

P

P

K

K

G

G

V

V

M

L

Y

Y

H

S

H

H

R

R

G

S

A

T

Y

V

L

L

Q
x
H

A

S

L

F

A

A

M

S

V

N

A

T

H

R

-

D

-

V

T

I

G

G

L

Y

S

S

P

A

S

M

A

D

V

V

H

V

L

M

W

P

T

V

L

V

P

P

M

M

F

F

H
|
H

C

V

N

N

G

A

W

W

C

G

F

S

P

P

W

Y

A

G

V

C

T

A

A

M

A

S

A

G

A

A

T

Q

H

M

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V

C

L

L

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-

G

P

P

K

D

V

L

E

H

P

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A

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E

A

V

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L

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T

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A

E

M

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G

A

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P

T

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-

G

M

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A

A

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H

A

A

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A

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A

C

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-

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K

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L

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R

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A

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G

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G
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G

A
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A

P
x
A

P

C

T

P

P

P

A

S

I

M

L

I

R

A

R

T

M

F

G

R

E

E

-

K

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G

F

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D

V

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T

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H
|
H

L
x
A

Y
x
W

G
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G

L
x
M

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x
S

E
|
E

T

M

-

S

-

P

F

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G

P

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A

A

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N

V

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L

A

P

A

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K

R

A

E

R

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Q

G

G

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R

G

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P

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F

I

-

S

G

C

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Q

E

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R

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V

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D

D

G

G

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D

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G

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I

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A

M

L

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G

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H

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G

S

Y

Y

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K

E

D

A

-

T

-

R

Q

Q

E

A

L

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Q

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D

G

G

W

W

F

F

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A

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G

G

D
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D

L

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A

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D

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G

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S

K

K

D

D

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I

I

I
x
K

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G

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E

N
x
W

I

I

A

S

S

S

V

V

E

E

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L

E

E

Q

N

A

I

I

A

A

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A

H

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P

A

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F

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A

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H

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K

W

W

G

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R

P

P

A

L

Y

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V

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H

A

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G

A

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D

V

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E

S

A

E

E

L

L

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L

A

E

H

F

V

F

R

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E

G

R

K

L

I

A

A

H

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F

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K

Q

A

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P

K

D

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K

V

V

F

F

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V

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D

-

A

L

L

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P

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S

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active site: T185 (= T165), H202 (≠ Q185), H228 (= H209), S326 (≠ T303), E327 (= E304), K435 (≠ I415), W440 (≠ N420), K520 (= K499)
binding adenosine-5'-diphosphate: H228 (= H209), G300 (= G278), A301 (= A279), A302 (≠ P280), H321 (= H298), A322 (≠ L299), W323 (≠ Y300), G324 (= G301), M325 (≠ L302), S326 (≠ T303), Q356 (= Q333), D414 (= D394), R429 (= R409), K520 (= K499)

P9WQ37 Long-chain-fatty-acid--CoA ligase FadD13; Fatty acyl-CoA ligase; FACL; FACL13; Fatty acyl-CoA synthetase; ACS; FACS; Very-long-chain fatty-acyl-CoA synthetase; ACSVL; EC 6.2.1.3 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 4 papers)
35% identity, 90% coverage: 35:508/525 of query aligns to 29:496/503 of P9WQ37

query
sites
P9WQ37

R

R

W

M

T

T

Y

Y

T

A

Q

Q

L

M

H

N

D

A

R

L

C

A

R

N

R

R

L

C

A

A

G

D

G

V

L

L

A

T

A

A

-

L

-

G

P

I

A

A

E

K

G

G

R

D

P

R

V

V

A

A

V

L

L

L

A

M

A

P

N

N

T

S

H

V

V

E

L

F

L

C

E

C

A

L

N

F

F

Y

G

G

V

A

P

A

W

K

A

L

G

G

V

A

P

V

L

A

V

V

A

P

V

I

N

N

T

T

R

R

L

L

S

A

A

A

R

P

E

E

V

V

A

S

Y

F

I

I

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L

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S

H

D

C

S

G

G

A

S

R

K

V

V

L

V

V

I

H

Y

D

G

-

A

-

P

-

S

-

A

P

P

A

V

F

I

D

D

D

A

L

I

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R

A

A

Q

Q

A

A

R

-

G

-

E

-

L

-

A

-

E

D

P

P

V

G

V

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-

V

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-

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-

W

I

I

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G

A

A

G

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A

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E

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A

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G

A

A

A

-

A

-

D

E

E

P

P

M

A

A

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C

P

G

A

G

D

D

E

D

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N

A

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-

L

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I

I

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M

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Y

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S

G

G

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G

G

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K
|
K

G

G

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V

M

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Y

H

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H

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A

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A

A

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A

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M

W

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A

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I

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x
R

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x
R

A

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R

H

L

L

L

W

L

T

P

L

L

P

P

M

M

F

F

H

H

C
x
V

N

A

G
x
A

W

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x
T

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W

I

A

F

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A

A

M

A

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A

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W

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x
R

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I

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G

E

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G

A

A

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A

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A

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A

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A

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A

A

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x
A

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C

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-

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-

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A

A

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R

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K

A

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A

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W

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D

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S

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G

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W

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K

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V

V

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A

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A

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Q

K172 (= K173) mutation to A: Slight reduction of the fatty acyl-CoA ligase activity. Slight increase of susceptibility to proteolysis.
R195 (≠ S196) mutation to A: Alteration of the strength of the membrane binding; when associated with A-9; A-17; A-197 and A-244.
R197 (≠ S198) mutation to A: Alteration of the strength of the membrane binding; when associated with A-9; A-17; A-195 and A-244.
V209 (≠ C210) mutation to D: Strong reduction of the fatty acyl-CoA ligase activity. No significant change in the total expression level, however the cytoplasmic expression is reduced. Slight increase of susceptibility to proteolysis.
A211 (≠ G212) mutation to G: Slight increase of the fatty acyl-CoA ligase activity. Reduced rate of proteolytic degradation.
T214 (≠ F215) mutation to W: Shows a marked decrease in the activity with lauric and palmitic acid (C12 and C16 fatty acid) with a simultaneous increase in the activity with caprylic acid (C8 fatty acid).
R244 (≠ G245) mutation to A: Alteration of the strength of the membrane binding; when associated with A-17; A-195; A-195 and A-197.
A302 (≠ G301) mutation to G: Slight increase of the fatty acyl-CoA ligase activity. Reduced rate of proteolytic degradation.; mutation to W: Does not show activity with small, medium or long acyl chains.
W377 (= W389) mutation to A: Strong reduction of the fatty acyl-CoA ligase activity. Enhanced affinity towards palmitic acid binding. No significant change in the total expression level, however the cytoplasmic expression is low. Slight increase of susceptibility to proteolysis.
D382 (= D394) mutation to A: Strong reduction of the fatty acyl-CoA ligase activity. No significant change in the total expression level, however the cytoplasmic expression is reduced.
R397 (= R409) mutation to A: Reduction of binding affinity for fatty acids.
S404 (= S416) mutation to A: Slight reduction of the fatty acyl-CoA ligase activity. Enhanced affinity towards palmitic acid binding.
G406 (= G418) mutation to L: No effect on the formation of acyl-adenylate intermediate. However, it shows very poor catalytic efficiency to form acyl-CoA.
K487 (= K499) mutation to A: Strong reduction of the fatty acyl-CoA ligase activity. Reduction of binding affinity for ATP.

Sites not aligning to the query:

9 R→A: Alteration of the strength of the membrane binding; when associated with A-9; A-195; A-197 and A-244.
17 R→A: Alteration of the strength of the membrane binding; when associated with A-9; A-17; A-197 and A-244.

8i3iA Acyl-acp synthetase structure bound to amp-pnp in the presence of mgcl2
31% identity, 90% coverage: 35:507/525 of query aligns to 35:519/522 of 8i3iA

query
sites
8i3iA

R

R

W

H

T

S

Y

Y

T

K

Q

T

L

F

H

H

D

D

R

R

C

V

R

R

R

Q

L

F

A

A

G

N

G

A

L

L

A

T

A

K

P

M

-

G

-

V

A

K

E

K

G

G

R

D

P

T

V

V

A

A

V

V

L

M

A

D

A

Y

N

D

T

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binding phosphoaminophosphonic acid-adenylate ester: T172 (= T165), G174 (= G167), T175 (= T168), T176 (= T169), K180 (= K173), G293 (= G278), A294 (= A279), A295 (≠ P280), Y315 (= Y300), M317 (≠ L302), S318 (≠ T303), D408 (= D394), R423 (= R409)

8i6mA Acyl-acp synthetase structure bound to amp-c18:1
31% identity, 90% coverage: 35:507/525 of query aligns to 33:525/528 of 8i6mA

query
sites
8i6mA

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