SitesBLAST
Comparing WP_009153233.1 NCBI__GCF_000244955.1:WP_009153233.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q8P3K2 L-fuconate dehydratase; FucD; EC 4.2.1.68 from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25) (see paper)
67% identity, 100% coverage: 1:424/425 of query aligns to 1:424/441 of Q8P3K2
- GSD 22:24 (= GSD 22:24) binding substrate
- Y32 (= Y32) binding substrate
- K218 (= K218) binding substrate
- K220 (= K220) mutation to A: Inactive.
- D248 (= D248) binding Mg(2+)
- N250 (= N250) binding substrate
- E274 (= E274) binding Mg(2+); binding substrate
- E301 (= E301) binding Mg(2+); binding substrate
- HAG 351:353 (= HAG 351:353) binding substrate
- E382 (= E382) binding substrate
1yeyC Crystal structure of l-fuconate dehydratase from xanthomonas campestris pv. Campestris str. Atcc 33913
67% identity, 100% coverage: 1:424/425 of query aligns to 1:424/435 of 1yeyC
- active site: T55 (= T55), T190 (= T190), K218 (= K218), K220 (= K220), D248 (= D248), N250 (= N250), E274 (= E274), G300 (= G300), E301 (= E301), D324 (= D324), P350 (= P350), H351 (= H351), A352 (= A352), D368 (= D368), K375 (≠ S375), E382 (= E382)
- binding magnesium ion: D248 (= D248), E274 (= E274), E301 (= E301)
2hxtA Crystal structure of l-fuconate dehydratase from xanthomonas campestris liganded with mg++ and d-erythronohydroxamate (see paper)
67% identity, 99% coverage: 4:424/425 of query aligns to 3:423/434 of 2hxtA
- active site: T54 (= T55), T189 (= T190), K217 (= K218), K219 (= K220), D247 (= D248), N249 (= N250), E273 (= E274), G299 (= G300), E300 (= E301), D323 (= D324), P349 (= P350), H350 (= H351), A351 (= A352), D367 (= D368), K374 (≠ S375), E381 (= E382)
- binding (2r,3r)-n,2,3,4-tetrahydroxybutanamide: G21 (= G22), D23 (= D24), Y31 (= Y32), K217 (= K218), K219 (= K220), E300 (= E301), H350 (= H351), G352 (= G353), E381 (= E382)
- binding magnesium ion: D247 (= D248), E273 (= E274), E300 (= E301)
2hxuA Crystal structure of k220a mutant of l-fuconate dehydratase from xanthomonas campestris liganded with mg++ and l-fuconate (see paper)
67% identity, 99% coverage: 4:424/425 of query aligns to 3:423/434 of 2hxuA
- active site: T54 (= T55), T189 (= T190), K217 (= K218), A219 (≠ K220), D247 (= D248), N249 (= N250), E273 (= E274), G299 (= G300), E300 (= E301), D323 (= D324), P349 (= P350), H350 (= H351), A351 (= A352), D367 (= D368), K374 (≠ S375), E381 (= E382)
- binding 6-deoxy-L-galactonic acid: G21 (= G22), D23 (= D24), Y31 (= Y32), W193 (= W194), K217 (= K218), D247 (= D248), E300 (= E301), H350 (= H351), G352 (= G353), E381 (= E382)
- binding magnesium ion: D247 (= D248), E273 (= E274), E300 (= E301)
Q7L5Y1 Mitochondrial enolase superfamily member 1; Antisense RNA to thymidylate synthase; rTS; L-fuconate dehydratase; EC 4.2.1.68 from Homo sapiens (Human) (see 5 papers)
53% identity, 100% coverage: 2:424/425 of query aligns to 4:428/443 of Q7L5Y1
- M145 (≠ E143) to T: in dbSNP:rs2612086
- S148 (≠ D146) modified: Phosphoserine
- D250 (= D248) binding Mg(2+)
- E276 (= E274) binding Mg(2+)
- E305 (= E301) binding Mg(2+)
Sites not aligning to the query:
- 1:27 mutation Missing: Impairs protein solubility. Abolishes catalytic activity.
4a35A Crystal structure of human mitochondrial enolase superfamily member 1 (enosf1) (see paper)
53% identity, 100% coverage: 2:424/425 of query aligns to 5:429/441 of 4a35A
- active site: K221 (= K218), K223 (= K220), D251 (= D248), N253 (= N250), E277 (= E274), E306 (= E301), D329 (= D324), H356 (= H351), S380 (= S375), E387 (= E382)
- binding magnesium ion: Y188 (= Y185), D251 (= D248), E277 (= E274), E306 (= E301), Y374 (≠ F369)
4ip5A Crystal structure of l-fuconate dehydratase from silicibacter sp. Tm1040 liganded with mg and d-erythronohydroxamate
58% identity, 97% coverage: 3:414/425 of query aligns to 2:411/421 of 4ip5A
- active site: K217 (= K220), D245 (= D248), N247 (= N250), E271 (= E274), E298 (= E301), D321 (= D324), H348 (= H351), E379 (= E382)
- binding (2r,3r)-n,2,3,4-tetrahydroxybutanamide: G21 (= G22), D23 (= D24), Y31 (= Y32), K215 (= K218), K217 (= K220), D245 (= D248), N247 (= N250), E298 (= E301), H348 (= H351)
- binding magnesium ion: D245 (= D248), E271 (= E274), E298 (= E301)
4ip4A Crystal structure of l-fuconate dehydratase from silicibacter sp. Tm1040 liganded with mg
58% identity, 97% coverage: 3:414/425 of query aligns to 2:411/421 of 4ip4A
4h19A Crystal structure of an enolase (mandelate racemase subgroup, target efi-502087) from agrobacterium tumefaciens, with bound mg and d- ribonohydroxamate, ordered loop
30% identity, 100% coverage: 3:425/425 of query aligns to 2:370/372 of 4h19A
- active site: I20 (≠ L20), T51 (= T55), T143 (= T190), K172 (= K218), K174 (= K220), D203 (= D248), N205 (= N250), E229 (= E274), G254 (= G300), E255 (= E301), Q276 (= Q322), D278 (= D324), H305 (= H351), A306 (= A352), G307 (= G353), E327 (= E382)
- binding (2R,3R,4R)-N,2,3,4,5-pentakis(oxidanyl)pentanamide: D22 (= D24), H25 (≠ N27), H52 (≠ I56), K172 (= K218), K174 (= K220), D203 (= D248), N205 (= N250), E229 (= E274), E255 (= E301), H305 (= H351), E327 (= E382)
- binding calcium ion: D268 (≠ Q314), H298 (≠ F344)
- binding magnesium ion: D203 (= D248), E229 (= E274), E255 (= E301)
Q8ZL58 L-talarate/galactarate dehydratase; TalrD/GalrD; StTGD; EC 4.2.1.156; EC 4.2.1.42 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
31% identity, 72% coverage: 45:351/425 of query aligns to 71:328/398 of Q8ZL58
- KR 82:83 (≠ IG 56:57) binding substrate
- K195 (= K218) binding substrate
- K197 (= K220) active site, Proton acceptor; mutation to A: Loss of dehydration activity on both L-talarate and galactarate and loss of epimerization activity.
- D226 (= D248) binding Mg(2+)
- N228 (= N250) binding substrate
- E252 (= E274) binding Mg(2+)
- E278 (= E301) binding Mg(2+)
- H328 (= H351) active site, Proton donor/acceptor; mutation H->N,A: Loss of dehydration activity on both L-talarate and galactarate and loss of epimerization activity.
Sites not aligning to the query:
- 46:48 binding substrate
- 348 binding substrate
2pp1A Crystal structure of l-talarate/galactarate dehydratase from salmonella typhimurium lt2 liganded with mg and l-lyxarohydroxamate (see paper)
31% identity, 72% coverage: 45:351/425 of query aligns to 68:325/395 of 2pp1A