SitesBLAST
Comparing WP_009153576.1 NCBI__GCF_000244955.1:WP_009153576.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 11 hits to proteins with known functional sites (download)
6fahE Molecular basis of the flavin-based electron-bifurcating caffeyl-coa reductase reaction (see paper)
34% identity, 99% coverage: 1:319/321 of query aligns to 64:388/393 of 6fahE
- binding flavin-adenine dinucleotide: L180 (= L113), R200 (≠ C131), M281 (≠ R212), G282 (= G213), R307 (= R238), A308 (≠ P239), Q320 (= Q251), V321 (= V252), G322 (= G253), Q323 (≠ K254), T324 (≠ S255), G337 (= G268), I338 (= I269), S339 (= S270), Q343 (≠ E274), H344 (= H275), N358 (= N289), K359 (≠ T290), L377 (= L308)
Sites not aligning to the query:
- binding iron/sulfur cluster: 7, 10, 13, 17, 18, 35, 36, 37, 38, 41, 45, 49
5ol2A The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
36% identity, 98% coverage: 4:319/321 of query aligns to 3:324/331 of 5ol2A
- binding calcium ion: E75 (= E72), D188 (≠ E182)
- binding flavin-adenine dinucleotide: T117 (≠ A114), R136 (≠ C131), I147 (≠ V142), G216 (= G211), R217 (= R212), G218 (= G213), S242 (= S237), R243 (= R238), A244 (≠ P239), Q256 (= Q251), V257 (= V252), G258 (= G253), T260 (≠ S255), G273 (= G268), I274 (= I269), S275 (= S270), A277 (= A272), Q279 (≠ E274), H280 (= H275), N294 (= N289), K295 (≠ T290), D312 (= D307), V313 (≠ L308)
7qh2A Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
32% identity, 98% coverage: 5:320/321 of query aligns to 13:332/337 of 7qh2A
- binding flavin-adenine dinucleotide: L125 (= L113), T126 (≠ A114), R144 (≠ Q132), I155 (≠ V142), R224 (= R212), G225 (= G213), T249 (≠ S237), R250 (= R238), Q263 (= Q251), I264 (≠ V252), G265 (= G253), L266 (≠ K254), S267 (= S255), G280 (= G268), I281 (= I269), S282 (= S270), Q286 (≠ E274), N301 (= N289), S302 (≠ T290), D303 (= D291), D319 (= D307), L320 (= L308)
P53571 Electron transfer flavoprotein subunit alpha; Alpha-ETF; Electron transfer flavoprotein large subunit; ETFLS from Methylophilus methylotrophus (Bacterium W3A1) (see 2 papers)
35% identity, 96% coverage: 4:311/321 of query aligns to 3:311/321 of P53571
- R211 (= R212) binding FAD
- SR 236:237 (= SR 237:238) binding FAD
- QVGQS 250:254 (≠ QVGKS 251:255) binding FAD
- 268:275 (vs. 268:275, 63% identical) binding FAD
- N289 (= N289) binding FAD
- DI 307:308 (≠ DL 307:308) binding FAD
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
4kpuA Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
32% identity, 99% coverage: 4:320/321 of query aligns to 12:333/338 of 4kpuA
- binding flavin-adenine dinucleotide: L125 (= L113), R144 (≠ C131), I155 (≠ V142), G224 (= G211), R225 (= R212), G226 (= G213), S250 (= S237), R251 (= R238), A252 (≠ P239), Q264 (= Q251), V265 (= V252), G266 (= G253), Q267 (≠ K254), S268 (= S255), G281 (= G268), I282 (= I269), S283 (= S270), S285 (≠ A272), Q287 (≠ E274), H288 (= H275), N302 (= N289), K303 (≠ T290), D320 (= D307), A321 (≠ L308)
3clrD Crystal structure of the r236a etf mutant from m. Methylotrophus (see paper)
35% identity, 96% coverage: 4:311/321 of query aligns to 2:310/319 of 3clrD
- binding flavin-adenine dinucleotide: G209 (= G211), R210 (= R212), G211 (= G213), S235 (= S237), A236 (≠ R238), P237 (= P239), Q249 (= Q251), V250 (= V252), G251 (= G253), Q252 (≠ K254), S253 (= S255), G267 (= G268), I268 (= I269), S269 (= S270), S271 (≠ A272), Q273 (≠ E274), H274 (= H275), N288 (= N289), T289 (= T290), D306 (= D307), I307 (≠ L308)
P13804 Electron transfer flavoprotein subunit alpha, mitochondrial; Alpha-ETF from Homo sapiens (Human) (see 6 papers)
39% identity, 61% coverage: 125:321/321 of query aligns to 142:332/333 of P13804
- T171 (≠ G156) to I: decreased protein stability; dbSNP:rs1801591
- R223 (= R212) binding FAD
- S248 (= S237) binding FAD
- R249 (= R238) mutation to A: Loss of electron transfer activity.
- VGQT 263:266 (≠ VGKS 252:255) binding FAD
- T266 (≠ S255) to M: in GA2A; decreased electron transfer activity; dbSNP:rs119458970
- SGAIQH 281:286 (≠ SGAPEH 270:275) binding FAD
- N300 (= N289) binding FAD
- DL 318:319 (= DL 307:308) binding FAD
Sites not aligning to the query:
- 20:204 Domain I
- 116 G → R: in GA2A; impaired protein stability and loss of electron transfer activity; dbSNP:rs119458971
- 205:333 Domain II
2a1uA Crystal structure of the human etf e165betaa mutant (see paper)
39% identity, 61% coverage: 125:321/321 of query aligns to 124:314/315 of 2a1uA
- binding flavin-adenine dinucleotide: G204 (= G211), R205 (= R212), S230 (= S237), R231 (= R238), A232 (≠ P239), Q244 (= Q251), V245 (= V252), G246 (= G253), T248 (≠ S255), G261 (= G268), I262 (= I269), S263 (= S270), A265 (= A272), Q267 (≠ E274), H268 (= H275), N282 (= N289), K283 (≠ T290), D300 (= D307), L301 (= L308)
1efpA Electron transfer flavoprotein (etf) from paracoccus denitrificans (see paper)
39% identity, 58% coverage: 133:319/321 of query aligns to 126:307/307 of 1efpA
- binding flavin-adenine dinucleotide: G199 (= G211), R200 (= R212), G201 (= G213), S225 (= S237), R226 (= R238), A227 (≠ P239), Q239 (= Q251), V240 (= V252), G241 (= G253), T243 (≠ S255), G256 (= G268), I257 (= I269), S258 (= S270), A260 (= A272), Q262 (≠ E274), H263 (= H275), N277 (= N289), K278 (≠ T290), D295 (= D307), L296 (= L308)
7koeB Electron bifurcating flavoprotein fix/etfabcx (see paper)
31% identity, 99% coverage: 5:321/321 of query aligns to 7:329/336 of 7koeB
- binding flavin-adenine dinucleotide: T121 (≠ A114), R140 (≠ C131), T142 (≠ V133), G219 (= G211), K220 (≠ R212), G221 (= G213), S245 (= S237), R246 (= R238), A247 (≠ P239), Q259 (= Q251), V260 (= V252), G261 (= G253), Q262 (≠ K254), T263 (≠ S255), G276 (= G268), S278 (= S270), Q282 (≠ E274), H283 (= H275), N297 (= N289), I298 (≠ T290), L316 (= L308)
5ow0A Crystal structure of an electron transfer flavoprotein from geobacter metallireducens (see paper)
31% identity, 79% coverage: 66:320/321 of query aligns to 48:292/292 of 5ow0A
- binding flavin-adenine dinucleotide: G183 (= G211), R184 (= R212), G185 (= G213), S209 (= S237), R210 (= R238), Q223 (= Q251), I224 (≠ V252), G225 (= G253), T227 (≠ S255), G240 (= G268), V241 (≠ I269), S242 (= S270), A244 (= A272), Q246 (≠ E274), H247 (= H275), N261 (= N289), K262 (≠ T290), D279 (= D307), Y280 (≠ L308)
Query Sequence
>WP_009153576.1 NCBI__GCF_000244955.1:WP_009153576.1
MSDNVLVLAEHIGGQLSESSYELLGKAKELAAAWGGKAEVALFGPSDLVSQVEGADVVVS
MDHPALADYTCEGYETALLSVLAERAPRLVLLSNATVGLDLGAALSVLWDAPLAAYVGDL
RAEGDTPVATCQVMGGKVMAEVELAGERGICTVLAGAFPAAAGQAPAVSQEVVTAAAPDE
LEKLRMSLARVIEPEGGDVNITDAELLVSVGRGIDSQDNLELVQELADALGAPLSASRPV
IDSGWLPKTRQVGKSGLKVKPKAYLAFGISGAPEHLEGMRDAELIIACNTDENAPIFDVA
HYGSAEDLFDLVPALVDKIKE
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory