SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_009156574.1 NCBI__GCF_000244955.1:WP_009156574.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P9WQD1 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
66% identity, 99% coverage: 1:651/659 of query aligns to 1:650/651 of P9WQD1

query
sites
P9WQD1

M

M

T

S

E

E

Q

S

S

T

P

P

A

E

L

V

D

-

N

-

L

-

T

-

E

-

S

S

R

S

T

S

F

Y

P

P

S

P

E

A

E

H

F

F

A

A

A

E

R

H

A

A

N

N

A

A

T

R

P

A

E

E

W

L

Y

Y

A

R

E

E

A

A

E

E

Q

E

D

D

R

R

E

L

A

A

F

F

W

W

A

A

R

K

Q

Q

A

A

E

N

R

R

L

L

H

S

W

W

D

T

T

T

R

P

W

F

S

T

Q

E

V

V

L

L

D

D

W

W

S

S

Q

G

A

A

P

P

F

F

A

A

K

K

W

W

F

F

V

V

G

G

K

E

L

L

N

N

V

V

A

A

Y

Y

N

N

C

C

V

V

D

D

R

R

H

H

V

V

E

E

A

A

G

G

H

H

G

G

D

D

Q

R

V

V

A

A

I

I

H

H

W

W

V

E

G

G

E

E

P

P

-

V

G

G

D

D

T

R

R

R

D

T

I

L

T

T

Y

Y

A

S

Q

D

L

L

K

L

D

A

E

E

V

V

C

S

K

K

A

A

N

N

A

A

L

L

T

T

S

D

L

L

G

G

V

L

T

V

A

A

N

G

D

D

R

R

V

V

A

A

I

I

Q

Y

L

L

Q

P

M

L

I

I

P

P

E

E

A

A

I

V

V

I

A

A

M

M

L

L

A

A

C

C

A

A

R

R

I

L

G

G

A

I

M

M

H

H

S

S

V

V

F

F

G

G

F

F

S

T

P

A

T

A

A

A

L

L

R

Q

S

A

R

R

V

I

D

V

D

D

A

A

Q

Q

A

A

K

K

V

L

I

I

T

T

S

A

D

D

G

G

Q

Q

Y

F

R

R

G

G

K

K

A

P

A

S

P

P

M

L

K

K

A

A

N

A

V

A

D

D

E

E

A

A

L

L

A

A

G

A

A

I

E

P

-

D

-

C

S

S

V

V

E

E

K

H

V

V

V

L

V

V

R

R

T

T

G

G

D

I

D

E

V

M

P

A

W

W

T

S

Q

E

E

G

R

R

D

D

L

L

W

W

H

H

E

H

L

V

V

V

D

G

P

S

Q

A

P

S

A

P

E

A

H

H

T

T

P

P

Q

E

A

P

F

F

D

D

S

S

E

E

H

H

P

P

L

L

Y

F

I

L

L

L

Y

Y

T

T

S

S

G

G

T

T

G

G

K

K

P

P

K

K

G

G

I

I

L

M

H

H

T

T

S

S

G

G

Y

Y

L

L

A

T

H

Q

V

C

A

C

Y

Y

T

T

H

M

H

R

A

T

V

I

F

F

D

D

H

V

K

K

P

P

G

D

E

S

D

D

V

V

Y

F

W

W

C

C

T

T

A

A

D

D

I

I

G

G

W

W

V

V

T

T

G

G

H

H

S

T

Y

Y

I

G

V

V

Y

Y

G

G

P

P

L

L

A

C

N

N

R

G

V

V

T

T

Q

E

V

V

V

L

Y

Y

E

E

G

G

T

T

P

P

N

D

T

T

P

P

H

D

E

R

G

H

R

R

H

H

W

F

E

Q

I

I

Q

E

K

K

Y

Y

K

G

V

V

S

T

I

I

Y

Y

T

T

A

A

P

P

T

T

L

L

I

I

R

R

T

M

F

F

M

M

K

K

W

W

G

G

N

R

E

E

I

I

P

P

A

D

K

S

Y

H

D

D

L

L

S

S

L

L

R

R

V

L

L

L

G

G

S

S

V

V

G

G

E

E

P

P

I

I

N

N

P

P

E

E

A

A

W

W

M

R

W

W

Y

Y

R

R

E

D

H

V

I

I

G

G

N

G

R

R

C

T

P

P

V

L

V

V

D

D

T

T

W

W

Q

Q

T

T

E

E

T

T

G

G

G

S

I

A

M

M

I

I

S

S

P

P

L

L

P

P

G

G

V

I

T

A

H

A

A

A

K

K

P

P

G

G

S

S

A

A

Q

M

R

T

P

P

L

L

P

P

G

G

I

I

S

S

A

A

K

K

V

I

V

V

D

D

T

H

G

G

-

D

-

P

-

L

-

P

-

H

T

T

E

E

V

G

G

A

K

Q

G

H

G

V

G

T

G

G

Y

Y

L

L

V

V

L

L

D

D

K

Q

P

P

W

W

P

P

G

S

M

M

L

L

R

R

G

G

I

I

W

W

G

G

D

D

D

P

E

A

R

R

Y

Y

K

W

E

H

T

S

Y

Y

W

W

S

S

R

K

F

F

A

S

E

D

Q

K

G

G

F

Y

Y

Y

F

F

A

A

G

G

D

D

G

G

A

A

K

R

Y

I

D

D

A

P

D

D

G

G

D

A

I

I

W

W

L

V

L

L

G

G

R

R

V

I

D

D

V

V

M

M

N

N

V

V

S

S

G

G

H

H

R

R

I

I

S

S

T

T

T

A

E

E

V

V

E

E

S

S

A

A

L

L

V

V

S

A

H

H

P

S

T

G

V

V

A

A

E

E

A

A

V

V

G

G

A

V

T

T

D

D

P

E

T

T

G

T

Q

Q

G

A

I

I

V

C

A

A

F

F

V

V

I

V

L

L

R

R

G

A

G

N

V

Y

A

A

E

P

D

H

E

D

A

R

G

T

G

A

T

E

E

E

A

-

I

-

Q

-

A

-

L

L

R

R

N

T

H

E

V

V

A

A

K

R

E

V

I

I

G

S

P

P

I

I

A

A

K

R

P

P

R

R

Q

D

I

V

M

H

V

V

P

P

E

E

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

L

L

R

R

D

D

V

V

A

A

E

E

N

N

R

R

E

E

I

L

G

G

D

D

V

T

T

S

T

T

L

L

A

L

D

D

S

P

S

T

V

V

M

F

D

D

L

A

I

I

S

R

S

A

G

A

K617 (= K618) modified: N6-acetyllysine; mutation to R: Complete loss of acetyl-coenzyme A synthetase activity.

Q89WV5 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110) (see paper)
53% identity, 95% coverage: 33:656/659 of query aligns to 25:646/648 of Q89WV5

query
sites
Q89WV5

E

E

W

M

Y

Y

A

A

E

R

A

S

E

I

Q

S

D

D

R

P

E

N

A

G

F

F

W

W

A

A

R

E

Q

Q

A

A

E

K

R

R

L

I

H

D

W

W

D

M

T

K

R

A

W

P

S

T

Q

K

V

I

L

E

D

N

W

V

S

S

Q

F

A

A

P

P

-

G

-

N

-

V

F

S

A

I

K

K

W

W

F

F

V

E

G

D

G

G

K

V

L

L

N

N

V

V

A

A

Y

H

N

N

C

C

V

I

D

D

R

R

H

H

V

L

E

H

A

K

G

-

H

R

G

A

D

N

Q

Q

V

T

A

A

I

I

H

I

W

W

V

E

G

G

E

D

-

D

P

P

G

S

D

Q

T

S

R

R

D

H

I

I

T

T

Y

Y

A

K

Q

E

L

L

K

H

D

D

E

E

V

V

C

C

K

R

A

M

A

A

N

N

A

I

L

L

T

R

S

T

L

R

G

N

V

V

T

K

A

K

N

G

D

D

R

R

V

V

A

T

I

I

Q

Y

L

L

Q

P

M

M

I

I

P

P

E

E

A

A

I

A

V

Y

A

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

M

I

H

H

S

S

V

V

F

F

G

A

G

G

F

F

S

S

P

P

T

D

A

S

L

L

R

A

S

Q

R

R

V

I

D

N

D

D

A

C

Q

Q

A

S

K

K

V

V

V

I

I

I

T

T

S

A

D

D

G

E

Q

G

Y

L

R

R

R

G

G

G

K

K

A

K

A

V

P

P

M

L

K

K

A

A

N

N

V

V

D

D

E

A

A

A

L

L

A

A

G

K

A

A

E

D

S

G

V

V

E

D

K

W

V

V

R

K

R

R

T

T

G

G

D

G

D

K

V

I

P

D

W

M

T

N

Q

P

E

T

R

R

D

D

L

L

W

W

W

Y

H

H

E

E

L

A

V

A

D

A

P

M

Q

V

P

T

A

T

E

E

H

C

T

P

P

V

Q

E

A

H

F

M

D

H

S

A

E

E

H

D

P

P

L

L

Y

F

I

I

L

L

Y

Y

T

T

S

S

G
|
G

T

S

T

T

G
|
G

K

Q

P

P

K
|
K

G

G

I

V

L

L

H

H

T

T

S

S

G

A

G

G

Y

Y

L

L

A

V

H

Y

V

A

A

A

Y

M

T

T

H

H

H

Q

A

Y

V

V

F

F

D

D

H

Y

K

H

P

D

G

G

E

-

D

D

V

I

Y

Y

W

W

C

C

T

T

A

A

D

D

I

V

G

G

W

W

V

V

T

T

G

G

H

H

S

S

Y

Y

I

I

V

L

Y

Y

G

G

P

P

L

L

A

A

N

N

R

G

V

A

T

T

Q

T

V

L

V

M

Y

F

E

E

G

G

T

V

P

P

N

N

T

Y

P

P

H

D

E

N

G

S

R

R

H

F

W

W

E

N

I

V

I

I

Q

D

K

K

Y

H

K

K

V

V

S

N

I

T

Y

F

Y

Y

T

T

A

A

P

P

T

T

L

A

I

I

R

R

T

A

F

L

M

M

K

Q

W

G

G

G

N

D

E

E

I

P

P

V

A

K

K

K

Y

T

D

S

L

R

S

A

S

S

L

L

R

R

V

L

L

L

G

G

S

S

V

V

G

G

E

E

P

P

I

I

N

N

P

P

E

E

A

A

W

W

M

E

W

W

Y

Y

R

H

E

R

H

V

I

V

G

G

G

E

N

D

R

R

C

C

P

P

V

I

V

V

D

D

T

T

W

W

Q

Q

T

T

E
|
E

T

T

G

G

I

I

M

L

I

I

S

T

P

P

L

L

P

P

G

G

V

A

T

T

H

K

A

L

K

K

P

P

G

G

S

S

A

A

Q

T

R

Q

P

P

L

F

P

F

G

G

I

V

S

V

A

P

K

E

V

I

V

V

D

D

D

A

T

D

G

G

T

K

E

V

V

L

G

E

K

G

G

E

G

T

G

G

Y

N

L

L

V

C

L

L

D

T

K

R

P

A

W

W

P

P

G

G

M

M

L

M

R

R

G

T

I

V

W

Y

G

G

D

D

D

H

E

A

R

R

Y

F

K

E

E

Q

T

T

Y

Y

W

F

S

S

R

T

F

Y

A

-

E

-

Q

K

G

G

F

K

Y

Y

F

F

A

T

G

G

D

D

G

G

A

C

K

R

Y

R

D

D

A

A

D

D

G

G

D

Y

I

Y

W

W

L

I

L

T

G

G

R

R

V

V

D

D

V

V

M

I

N

N

V

V

S

S

G

G

H

H

R

R

I

M

S

G

T

T

T

A

E

E

V

V

E

E

S

S

A

A

L

L

V

V

S

A

H

H

P

E

T

K

V

V

A

S

E

E

A

A

V

V

G

G

A

F

T

P

D

H

P

D

T

I

T

K

G

G

Q

Q

G

G

I

I

V

Y

A

A

F

Y

V

V

I

T

L

L

R

M

G

A

G

G

V

V

A

Q

E

P

D

T

E

E

A

D

G

L

G

R

T

K

E

E

A

L

I

V

Q

T

A

W

L

V

R

R

N

-

H

-

V

-

A

-

K

K

E

E

I

I

G

G

P

P

I

I

A

A

K

S

P

P

R

D

Q

Q

I

I

M

Q

V

F

V

A

P

P

E

G

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K

K

I

I

M

M

R

R

L

I

L

L

R

R

D

K

V

I

A

A

E

E

N

D

R

E

-

P

-

G

E

S

I

L

G

G

D

D

V

T

T

S

T

T

L

L

A

A

D

D

S

P

S

A

V

V

M

V

D

D

L

D

I

L

S

V

S

K

G

N

L

R

R

Q

S

N

G

K

K

K

G263 (= G268) mutation to I: Loss of activity.
G266 (= G271) mutation to I: Great decrease in activity.
K269 (= K274) mutation to G: Great decrease in activity.
E414 (= E420) mutation to Q: Great decrease in activity.

2p20A Acetyl-coa synthetase, r584a mutation (see paper)
51% identity, 93% coverage: 35:648/659 of query aligns to 24:635/641 of 2p20A

query
sites
2p20A

Y

Y

A

K

E

Q

A

S

E

I

Q

N

D

D

R

P

E

D

A

T

F

F

W

W

A

G

R

E

Q

Q

A

G

E

K

R

I

L

L

H

D

W

W

D

I

T

T

R

P

W

Y

S

Q

Q

K

V

V

L

K

D

N

W

T

S

S

Q

F

A

A

P

P

-

G

-

N

-

V

F

S

A

I

K

K

W

W

F

Y

V

E

G

D

G

G

K

T

L

L

N

N

V

L

A

A

Y

A

N

N

C

C

V

L

D

D

R

R

H

H

V

L

E

Q

A

E

G

-

H

N

G

G

D

D

Q

R

V

T

A

A

I

I

H

I

W

W

V

E

G

G

E

D

-

D

P

T

G

S

D

Q

T

S

R

K

D

H

I

I

T

S

Y

Y

A

R

Q

E

L

L

K

H

D

R

E

D

V

V

C

C

K

R

A

F

A

A

N

N

A

T

L

L

T

L

S

D

L

L

G

G

V

I

T

K

A

K

N

G

D

D

R

V

V

V

A

A

I

I

Q

Y

L

M

Q

P

M

M

I

V

P

P

E

E

A

A

I

A

V

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

M

V

H

H

S

S

V

V

V

I

F

F

G

G

F

F

S

S

P

P

T

E

A

A

L

V

R

A

S

G

R

R

V

I

D

I

D

D

A

S

Q

S

A

S

K

R

V

L

V

V

I

I

T

T

S

A

D

D

G

E

Q

G

Y

V

R

R

R

A

G

G

K

R

A

S

A

I

P

P

M

L

K

K

A

K

N

N

V

V

D

D

E

D

A

A

L

L

A

K

G

N

A

P

E

N

-

V

-

T

S

S

V

V

E

E

K

H

V

V

V

I

V

V

V

L

R

K

R

R

T

T

G

G

D

S

D

D

V

I

P

D

W

W

T

Q

Q

E

E

G

R

R

D

D

L

L

W

W

H

R

E

D

L

L

V

I

D

E

P

K

Q

A

P

S

A

P

E

E

H

H

T

Q

P

P

Q

E

A

A

F

M

D

N

S

A

E

E

H

D

P

P

L

L

Y

F

I

I

L

L

Y

Y

T
|
T

S

S

G

G

T

S

T

T

G

G

K

K

P

P

K

K

G

G

I

V

L

L

H

H

T

T

S

T

G

G

Y

Y

L

L

A

V

H

Y

V

A

A

A

Y

T

T

T

H

F

H

K

A

Y

V

V

F

F

D

D

H

Y

K

H

P

P

G

G

E

-

D

D

V

I

Y

Y

W

W

C

C

T

T

A

A

D

D

I

V

G

G

W

W

V

V

T

T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

A

A

N

C

R

G

V

A

T

T

Q

T

V

L

V

M

Y

F

E

E

G

G

T

V

P

P

N

N

T

W

P

P

H

T

E

P

G

A

R

R

H

M

W

C

E

Q

I

V

Q

D

K

K

Y

H

K

Q

V

V

S

N

I

I

Y

L

Y

Y

T

T

A

A

P

P

T

T

L

A

I

I

R

R

T

A

F

L

M

M

K

A

W

E

G

G

N

D

E

K

I

A

P

I

A

E

K

G

Y

T

D

D

L

R

S

S

L

L

R

R

V

I

L

L

G

G

S

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

A

A

W

W

M

E

W

W

Y

Y

R

W

E

K

H

K

I

I

G

G

G

K

N

E

R

K

C

C

P

P

V

V

D

D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

I

F

M

M

I

I

S

T

P

P

L

L

P

P

G

G

V

A

T

I

H

E

A

L

K

K

P

A

G

G

S

S

A

A

Q

T

R

R

P

P

L

F

P

F

G

G

I

V

S

Q

A

P

K

A

V

L

V

V

D

D

D

N

T

E

G

G

T

H

E

P

V

Q

G

E

K

G

G

A

G

T

G

E

G

G

Y

N

L

L

V

V

L

I

D

T

K

D

P

S

W

W

P

P

G

G

M

Q

L

A

R

R

G

T

I

L

W

F

G

G

D

D

D

H

E

E

R

R

Y

F

K

E

E

Q

T

T

Y

Y

W

F

S

S

R

T

F

F

A

-

E

-

Q

K

G

N

F

M

Y

Y

F

F

A

S

G

G

D
|
D

G

G

A

A

K

R

Y

R

D

D

A

E

D

D

G

G

D

Y

I

Y

W

W

L
x
I

L

T

G

G

R
|
R

V

V

D

D

V

V

M

L

N
|
N

V

V

S

S

G

G

H

H

R
|
R

I

L

S

G

T

T

T

A

E

E

V

I

E

E

S

S

A

A

L

L

V

V

S

A

H

H

P

P

T

K

V

I

A

A

E

E

A

A

V

V

G

G

A

I

T

P

D

H

P

A

T

I

T

K

G

G

Q

Q

G

A

I

I

V

Y

A

A

F

Y

V

V

I

T

L

L

R

N

G

H

G

G

V

-

A

-

E

-

D

-

E

E

A

E

G

P

G

S

T

P

E

E

A

L

I

Y

Q

A

A

E

L

V

R

R

N

N

H

W

V

V

A

A

K

K

E

E

I

I

G

G

P

P

I

L

A

A

K

T

P

P

R

D

Q

V

I

L

M

H

V

W

V

T

P

D

E

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

L

I

L

L

R

R

D

K

V

I

A

A

E

A

N

G

R

D

E

T

I

S

G

N

D

D

V

T

T

S

T

T

L

L

A

A

D

D

S

P

S

G

V

V

M

V

D

E

L

K

I

L

active site: T260 (= T266), T412 (= T419), E413 (= E420), N517 (= N526), R522 (= R531), K605 (= K618)
binding adenosine-5'-monophosphate-propyl ester: G383 (= G390), E384 (= E391), P385 (= P392), T408 (= T415), W409 (= W416), W410 (= W417), Q411 (= Q418), T412 (= T419), D496 (= D505), I508 (≠ L517), R511 (= R520), R522 (= R531)

Q8ZKF6 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
51% identity, 93% coverage: 35:648/659 of query aligns to 28:641/652 of Q8ZKF6

query
sites
Q8ZKF6

Y

Y

A

K

E

Q

A

S

E

I

Q

N

D

D

R

P

E

D

A

T

F

F

W

W

A

G

R

E

Q

Q

A

G

E

K

R

I

L

L

H

D

W

W

D

I

T

T

R

P

W

Y

S

Q

Q

K

V

V

L

K

D

N

W

T

S

S

Q

F

A

A

P

P

-

G

-

N

-

V

F

S

A

I

K

K

W

W

F

Y

V

E

G

D

G

G

K

T

L

L

N

N

V

L

A

A

Y

A

N

N

C

C

V

L

D

D

R

R

H

H

V

L

E

Q

A

E

G

-

H

N

G

G

D

D

Q

R

V

T

A

A

I

I

H

I

W

W

V

E

G

G

E

D

-

D

P

T

G

S

D

Q

T

S

R

K

D

H

I

I

T

S

Y

Y

A

R

Q

E

L

L

K

H

D

R

E

D

V

V

C

C

K

R

A

F

A

A

N

N

A

T

L

L

T

L

S

D

L

L

G

G

V

I

T

K

A

K

N

G

D

D

R

V

V

V

A

A

I

I

Q

Y

L

M

Q

P

M

M

I

V

P

P

E

E

A

A

I

A

V

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

M

V

H

H

S

S

V

V

V

I

F

F

G

G

F

F

S

S

P

P

T

E

A

A

L

V

R

A

S

G

R

R

V

I

D

I

D

D

A

S

Q

S

A

S

K

R

V

L

V

V

I

I

T

T

S

A

D

D

G

E

Q

G

Y

V

R

R

R

A

G

G

K
x
R

A

S

A

I

P

P

M

L

K

K

A

K

N

N

V

V

D

D

E

D

A

A

L

L

A

K

G

N

A

P

E

N

-

V

-

T

S

S

V

V

E

E

K

H

V

V

V

I

V

V

V

L

R

K

R

R

T

T

G

G

D

S

D

D

V

I

P

D

W

W

T

Q

Q

E

E

G

R

R

D

D

L

L

W

W

H

R

E

D

L

L

V

I

D

E

P

K

Q

A

P

S

A

P

E

E

H

H

T

Q

P

P

Q

E

A

A

F

M

D

N

S

A

E

E

H

D

P

P

L

L

Y

F

I

I

L

L

Y

Y

T

T

S

S

G

G

T

S

T

T

G

G

K

K

P

P

K

K

G

G

I

V

L

L

H

H

T

T

S

T

G

G

Y

Y

L

L

A

V

H

Y

V

A

A

A

Y

T

T

T

H

F

H

K

A

Y

V

V

F

F

D

D

H

Y

K

H

P

P

G

G

E

-

D

D

V

I

Y

Y

W

W

C

C

T

T

A

A

D

D

I

V

G

G

W

W

V

V

T
|
T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

A

A

N

C

R

G

V

A

T

T

Q

T

V

L

V

M

Y

F

E

E

G

G

T

V

P

P

N
|
N

T

W

P

P

H

T

E

P

G

A

R

R

H

M

W

C

E

Q

I

V

Q

D

K

K

Y

H

K

Q

V

V

S

N

I

I

Y

L

Y

Y

T

T

A
|
A

P

P

T

T

L

A

I

I

R

R

T

A

F

L

M

M

K

A

W

E

G

G

N

D

E

K

I

A

P

I

A

E

K

G

Y

T

D

D

L

R

S

S

L

L

R

R

V

I

L

L

G

G

S

S

V

V

G

G

E

E

P

P

I

I

N

N

P

P

E

E

A

A

W

W

M

E

W

W

Y

Y

R

W

E

K

H

K

I

I

G

G

G

K

N

E

R

K

C

C

P

P

V

V

D

D

T

T

W

W

Q

Q

T

T

E

E

T

T

G

G

I

F

M

M

I

I

S

T

P

P

L

L

P

P

G

G

V

A

T

I

H

E

A

L

K

K

P

A

G

G

S

S

A

A

Q

T

R

R

P

P

L

F

P

F

G

G

I

V

S

Q

A

P

K

A

V

L

V

V

D

D

D

N

T

E

G

G

T

H

E

P

V

Q

G

E

K

G

G

A

G

T

G

E

G

G

Y

N

L

L

V

V

L

I

D

T

K

D

P

S

W

W

P

P

G

G

M

Q

L

A

R

R

G

T

I

L

W

F

G

G

D

D

D

H

E

E

R

R

Y

F

K

E

E

Q

T

T

Y

Y

W

F

S

S

R

T

F

F

A

-

E

-

Q

K

G

N

F

M

Y

Y

F

F

A

S

G

G

D

D

G

G

A

A

K

R

Y

R

D

D

A

E

D

D

G

G

D

Y

I

Y

W

W

L

I

L

T

G

G

R

R

V

V

D
|
D

D

D

V

V

M

L

N

N

V

V

S
|
S

G
|
G

H

H

R
|
R

I

L

S

G

T

T

T

A

E

E

V

I

E

E

S

S

A

A

L

L

V

V

S

A

H

H

P

P

T

K

V

I

A

A

E

E

A

A

V

V

G

G

A

I

T

P

D

H

P

A

T

I

T

K

G

G

Q

Q

G

A

I

I

V

Y

A

A

F

Y

V

V

I

T

L

L

R

N

G

H

G

G

V

-

A

-

E

-

D

-

E

E

A

E

G

P

G

S

T

P

E

E

A

L

I

Y

Q

A

A

E

L

V

R

R

N

N

H

W

V

V

A
x
R

K

K

E

E

I

I

G

G

P

P

I

L

A

A

K

T

P

P

R

D

Q

V

I

L

M

H

V

W

V

T

P

D

E

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

L

I

L

L

R

R

D

K

V

I

A

A

-

A

-

G

E

D

N

T

R

S

E

N

I

L

G

G

D

D

V

T

T

S

T

T

L

L

A

A

D

D

S

P

S

G

V

V

M

V

D

E

L

K

I

L

R194 (≠ K198) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 3-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 2-fold reduction for CoA.
T311 (= T314) binding CoA
N335 (= N338) binding CoA
A357 (= A360) mutation to V: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 3-fold reduction for CoA.
D517 (= D522) mutation to G: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 10-fold reduction for CoA.; mutation to P: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold reduction in the affinity for ATP and 4.5-fold reduction for CoA.
S523 (= S528) binding CoA
G524 (= G529) mutation to L: No acetyl-coenzyme A synthetase activity.; mutation to S: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. Almost the same affinity as the wild-type for ATP, but 9-fold reduction in the affinity for CoA.
R526 (= R531) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 4-fold reduction for CoA.
R584 (≠ A593) binding CoA; mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 7-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 8-fold reduction for CoA.
K609 (= K618) modified: N6-acetyllysine; by Pat; mutation to A: No acetyl-coenzyme A synthetase activity.

5jrhA Crystal structure of salmonella enterica acetyl-coa synthetase (acs) in complex with camp and coenzyme a (see paper)
51% identity, 93% coverage: 35:648/659 of query aligns to 24:634/640 of 5jrhA

query
sites
5jrhA

Y

Y

A

K

E

Q

A

S

E

I

Q

N

D

D

R

P

E

D

A

T

F

F

W

W

A

G

R

E

Q

Q

A

G

E

K

R

I

L

L

H

D

W

W

D

I

T

T

R

P

W

Y

S

Q

Q

K

V

V

L

K

D

N

W

T

S

S

Q

F

A

A

P

P

-

G

-

N

-

V

F

S

A

I

K

K

W

W

F

Y

V

E

G

D

G

G

K

T

L

L

N

N

V

L

A

A

Y

A

N

N

C

C

V

L

D

D

R

R

H

H

V

L

E

Q

A

E

G

-

H

N

G

G

D

D

Q

R

V

T

A

A

I

I

H

I

W
|
W

V

E

G

G

E

D

-

D

P

T

G

S

D

Q

T

S

R

K

D

H

I

I

T

S

Y

Y

A

R

Q

E

L

L

K

H

D

R

E

D

V

V

C

C

K

R

A

F

A

A

N

N

A

T

L

L

T

L

S

D

L

L

G

G

V

I

T

K

A

K

N

G

D

D

R

V

V

V

A

A

I

I

Q

Y

L

M

Q

P

M

M

I

V

P

P

E
|
E

A

A

I

A

V

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

M

V

H

H

S

S

V

V

V

I

F
|
F

G
|
G

F

F

S

S

P

P

T

E

A

A

L

V

R

A

S
x
G

R

R

V

I

D

I

D

D

A

S

Q

S

A

S

K

R

V

L

V

V

I

I

T

T

S

A

D

D

G

E

Q

G

Y

V

R
|
R

R

A

G

G

K

R

A

S

A

I

P

P

M

L

K

K

A

K

N

N

V

V

D

D

E

D

A

A

L

L

A

K

G

N

A

P

E

N

-

V

-

T

S

S

V

V

E

E

K

H

V

V

V

I

V

V

V

L

R

K

R

R

T

T

G

G

D

S

D

D

V

I

P

D

W

W

T

Q

Q

E

E

G

R

R

D

D

L

L

W

W

H

R

E

D

L

L

V

I

D

E

P

K

Q

A

P

S

A

P

E

E

H

H

T

Q

P

P

Q

E

A

A

F

M

D

N

S

A

E

E

H

D

P

P

L

L

Y

F

I

I

L

L

Y

Y

T
|
T

S

S

G

G

T

S

T

T

G

G

K
|
K

P
|
P

K

K

G

G

I

V

L

L

H

H

T

T

S

T

G

G

Y

Y

L

L

A

V

H

Y

V

A

A

A

Y

T

T

T

H

F

H

K

A

Y

V

V

F

F

D

D

H

Y

K

H

P

P

G

G

E

-

D

D

V

I

Y

Y

W

W

C

C

T

T

A

A

D

D

I

V

G

G

W

W

V

V

T

T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

A

A

N

C

R

G

V

A

T

T

Q

T

V

L

V

M

Y

F

E

E

G

G

T

V

P

P

N

N

T

W

P

P

H

T

E

P

G

A

R

R

H

M

W

C

E

Q

I

V

Q

D

K

K

Y

H

K

Q

V

V

S

N

I

I

Y

L

Y

Y

T

T

A

A

P

P

T

T

L

A

I

I

R

R

T

A

F

L

M

M

K

A

W

E

G

G

N

D

E

K

I

A

P

I

A

E

K

G

Y

T

D

D

L

R

S

S

L

L

R

R

V

I

L

L

G

G

S

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

A

A

W

W

M

E

W

W

Y

Y

R

W

E

K

H

K

I

I

G

G

G

K

N

E

R

K

C

C

P

P

V

V

D

D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

I

F

M

M

I

I

S

T

P

P

L

L

P

P

G

G

V

A

T

I

H

E

A

L

K

K

P

A

G

G

S

S

A

A

Q

T

R

R

P

P

L

F

P

F

G

G

I

V

S

Q

A

P

K

A

V

L

V

V

D

D

D

N

T

E

G

G

T

H

E

P

V

Q

G

E

K

G

G

A

G

T

G

E

G

G

Y

N

L

L

V

V

L

I

D

T

K

D

P

S

W

W

P

P

G

G

M

Q

L

A

R

R

G

T

I

L

W

F

G

G

D

D

D

H

E

E

R

R

Y

F

K

E

E

Q

T

T

Y

Y

W

F

S

S

R

T

F

F

A

-

E

-

Q

K

G

N

F

M

Y

Y

F

F

A

S

G

G

D
|
D

G

G

A

A

K

R

Y

R

D

D

A

E

D

D

G

G

D

Y

I

Y

W

W

L
x
I

L

T

G

G

R

R

V

V

D

D

V

V

M

L

N
|
N

V

V

S
|
S

G

G

H

H

R
|
R

I

L

S

G

T

T

T

A

E

E

V

I

E

E

S

S

A

A

L

L

V
|
V

S

A

H
|
H

P

P

T

K

V
x
I

A

A

E

E

A

A

V

V

G

G

A

I

T

P

D

H

P

A

T

I

T

K

G

G

Q

Q

G

A

I

I

V

Y

A

A

F

Y

V

V

I

T

L

L

R

N

G

H

G

G

V

-

A

-

E

-

D

-

E

E

A

E

G

P

G

S

T

P

E

E

A

L

I

Y

Q

A

A

E

L

V

R

R

N

N

H

W

V

V

A
x
R

K

K

E

E

I

I

G

G

P
|
P

I

L

A

A

K

T

P

P

R

D

Q

V

I

L

M

H

V

W

V

T

P

D

E

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

L

I

L

L

R

R

D

K

V

I

A

A

E

A

N

G

R

-

E

D

I

L

G

G

D

D

V

T

T

S

T

T

L

L

A

A

D

D

S

P

S

G

V

V

M

V

D

E

L

K

I

L

active site: T260 (= T266), T412 (= T419), E413 (= E420), N517 (= N526), R522 (= R531), K605 (= K618)
binding (r,r)-2,3-butanediol: W93 (= W102), E140 (= E148), G169 (≠ S177), K266 (= K272), P267 (= P273)
binding adenosine-3',5'-cyclic-monophosphate: G383 (= G390), E384 (= E391), P385 (= P392), T408 (= T415), W409 (= W416), W410 (= W417), Q411 (= Q418), T412 (= T419), D496 (= D505), I508 (≠ L517), N517 (= N526), R522 (= R531)
binding coenzyme a: F159 (= F167), G160 (= G168), G161 (= G169), R187 (= R195), S519 (= S528), R580 (≠ A593), P585 (= P598)
binding magnesium ion: V533 (= V542), H535 (= H544), I538 (≠ V547)

P27550 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Escherichia coli (strain K12) (see paper)
50% identity, 95% coverage: 23:646/659 of query aligns to 12:639/652 of P27550

query
sites
P27550

E

N

F

I

A

A

A

D

R

R

A

C

N

L

A

I

T

N

P

P

E

Q

W

Q

Y

Y

A

E

E

A

A

M

E

Y

Q

Q

D

Q

R

S

-

I

-

N

-

V

-

P

E

D

A

T

F

F

W

W

A

G

R

E

Q

Q

A

G

E

K

R

I

L

L

H

D

W

W

D

I

T

K

R

P

W

Y

S

Q

Q

K

V

V

L

K

D

N

W

T

S

S

Q

F

A

A

P

P

-

G

-

N

-

V

F

S

A

I

K

K

W

W

F

Y

V

E

G

D

G

G

K

T

L

L

N

N

V

L

A

A

Y

A

N

N

C

C

V

L

D

D

R

R

H

H

V

L

E

Q

A

E

G

-

H

N

G

G

D

D

Q

R

V

T

A

A

I

I

H

I

W

W

V

E

G

G

E

D

-

D

P

A

G

S

D

Q

T

S

R

K

D

H

I

I

T

S

Y

Y

A

K

Q

E

L

L

K

H

D

R

E

D

V

V

C

C

K

R

A

F

A

A

N

N

A

T

L

L

T

L

S

E

L

L

G

G

V

I

T

K

A

K

N

G

D

D

R

V

V

V

A

A

I

I

Q

Y

L

M

Q

P

M

M

I

V

P

P

E

E

A

A

I

A

V

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

M

V

H

H

S

S

V

V

V

I

F

F

G

G

F

F

S

S

P

P

T

E

A

A

L

V

R

A

S

G

R

R

V

I

D

I

D

D

A

S

Q

N

A

S

K

R

V

L

V

V

I

I

T

T

S

S

D

D

G

E

Q

G

Y

V

R

R

R

A

G

G

K

R

A

S

A

I

P

P

M

L

K

K

A

K

N

N

V

V

D

D

E

D

A

A

L

L

A

K

G

N

A

P

E

N

-

V

-

T

S

S

V

V

E

E

K

H

V

V

V

L

R

K

R

R

T

T

G

G

D

G

D

K

V

I

P

D

W

W

T

Q

Q

E

E

G

R

R

D

D

L

L

W

W

H

H

E

D

L

L

V

V

D

E

P

Q

Q

A

P

S

A

D

E

Q

H

H

T

Q

P

A

Q

E

A

E

F

M

D

N

S

A

E

E

H

D

P

P

L

L

Y

F

I

I

L

L

Y

Y

T

T

S

S

G

G

T

S

T

T

G

G

K

K

P

P

K

K

G

G

I

V

L

L

H

H

T

T

S

T

G

G

Y

Y

L

L

A

V

H

Y

V

A

A

A

Y

L

T

T

H

F

H

K

A

Y

V

V

F

F

D

D

H

Y

K

H

P

P

G

G

E

-

D

D

V

I

Y

Y

W

W

C

C

T

T

A

A

D

D

I

V

G

G

W

W

V

V

T

T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

A

A

N

C

R

G

V

A

T

T

Q

T

V

L

V

M

Y

F

E

E

G

G

T

V

P

P

N

N

T

W

P

P

H

T

E

P

G

A

R

R

H

M

W

A

E

Q

I

V

Q

D

K

K

Y

H

K

Q

V

V

S

N

I

I

Y

L

Y

Y

T

T

A

A

P

P

T

T

L

A

I

I

R

R

T

A

F

L

M

M

K

A

W

E

G

G

N

D

E

K

I

A

P

I

A

E

K

G

Y

T

D

D

L

R

S

S

L

L

R

R

V

I

L

L

G

G

S

S

V

V

G

G

E

E

P

P

I

I

N

N

P

P

E

E

A

A

W

W

M

E

W

W

Y

Y

R

W

E

K

H

K

I

I

G

G

G

N

N

E

R

K

C

C

P

P

V

V

D

D

T

T

W

W

Q

Q

T

T

E

E

T

T

G

G

I

F

M

M

I

I

S

T

P

P

L

L

P

P

G

G

V

A

T

T

H

E

A

L

K

K

P

A

G

G

S

S

A

A

Q

T

R

R

P

P

L

F

P

F

G

G

I

V

S

Q

A

P

K

A

V

L

V

V

D

D

D

N

T

E

G

G

T

N

E

P

V

L

G

E

K

G

G

A

G

T

G

E

G

G

Y

S

L

L

V

V

L

I

D

T

K

D

P

S

W

W

P

P

G

G

M

Q

L

A

R

R

G

T

I

L

W

F

G

G

D

D

D

H

E

E

R

R

Y

F

K

E

E

Q

T

T

Y

Y

W

F

S

S

R

T

F

F

A

-

E

-

Q

K

G

N

F

M

Y

Y

F

F

A

S

G

G

D

D

G

G

A

A

K

R

Y

R

D

D

A

E

D

D

G

G

D

Y

I

Y

W

W

L

I

L

T

G

G

R

R

V

V

D

D

V

V

M

L

N

N

V

V

S

S

G

G

H

H

R

R

I

L

S

G

T

T

T

A

E

E

V

I

E

E

S

S

A

A

L

L

V

V

S

A

H

H

P

P

T

K

V

I

A

A

E

E

A

A

V

V

G

G

A

I

T

P

D

H

P

N

T

I

T

K

G

G

Q

Q

G

A

I

I

V

Y

A

A

F

Y

V

V

I

T

L

L

R

N

G

H

G

G

V

-

A

-

E

-

D

-

E

E

A

E

G

P

G

S

T

P

E

E

A

L

I

Y

Q

A

A

E

L

V

R

R

N

N

H

W

V

V

A

R

K

K

E

E

I

I

G

G

P

P

I

L

A

A

K

T

P

P

R

D

Q

V

I

L

M

H

V

W

V

T

P

D

E

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

L

I

L

L

R

R

D

K

V

I

A

A

-

A

-

G

E

D

N

T

R

S

E

N

I

L

G

G

D

D

V

T

T

S

T

T

L

L

A

A

D

D

S

P

S

G

V

V

M

V

D

E

K609 (= K618) modified: N6-acetyllysine; by autocatalysis

2p2fA Acetyl-coa synthetase, wild-type with acetate, amp, and coa bound (see paper)
51% identity, 93% coverage: 35:648/659 of query aligns to 23:631/637 of 2p2fA

query
sites
2p2fA

Y

Y

A

K

E

Q

A

S

E

I

Q

N

D

D

R

P

E

D

A

T

F

F

W

W

A

G

R

E

Q

Q

A

G

E

K

R

I

L

L

H

D

W

W

D

I

T

T

R

P

W

Y

S

Q

Q

K

V

V

L

K

D

N

W

T

S

S

Q

F

A

A

P

P

-

G

-

N

-

V

F

S

A

I

K

K

W

W

F

Y

V

E

G

D

G

G

K

T

L

L

N

N

V

L

A

A

Y

A

N

N

C

C

V

L

D

D

R

R

H

H

V

L

E

Q

A

E

G

-

H

N

G

G

D

D

Q

R

V

T

A

A

I

I

H

I

W

W

V

E

G

G

E

D

-

D

P

T

G

S

D

Q

T

S

R

K

D

H

I

I

T

S

Y

Y

A

R

Q

E

L

L

K

H

D

R

E

D

V

V

C

C

K

R

A

F

A

A

N

N

A

T

L

L

T

L

S

D

L

L

G

G

V

I

T

K

A

K

N

G

D

D

R

V

V

V

A

A

I

I

Q

Y

L

M

Q

P

M

M

I

V

P

P

E

E

A

A

I

A

V

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

M

V

H

H

S

S

V

V

V

I

F
|
F

G

G

F

F

S

S

P

P

T

E

A

A

L

V

R

A

S

G

R

R

V

I

D

I

D

D

A

S

Q

S

A

S

K

R

V

L

V

V

I

I

T

T

S

A

D

D

G

E

Q

G

Y

V

R
|
R

R

A

G

G

K

R

A

S

A

I

P

P

M

L

K

K

A

K

N

N

V

V

D

D

E

D

A

A

L

L

A

K

G

N

A

P

E

N

-

V

-

T

S

S

V

V

E

E

K

H

V

V

V

I

V

V

V

L

R

K

R

R

T

T

G

G

D

S

D

D

V

I

P

D

W

W

T

Q

Q

E

E

G

R

R

D

D

L

L

W

W

H

R

E

D

L

L

V

I

D

E

P

K

Q

A

P

S

A

P

E

E

H

H

T

Q

P

P

Q

E

A

A

F

M

D

N

S

A

E

E

H

D

P

P

L

L

Y

F

I

I

L

L

Y

Y

T
|
T

S

S

G

G

T

S

T

T

G

G

K

K

P

P

K

K

G

G

I

V

L

L

H

H

T

T

S

T

G

G

Y

Y

L

L

A

V

H

Y

V

A

A

A

Y

T

T

T

H

F

H

K

A

Y

V

V

F

F

D

D

H

Y

K

H

P

P

G

G

E

-

D

D

V

I

Y

Y

W

W

C

C

T

T

A

A

D

D

I

V

G

G

W
|
W

V

V

T
|
T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

A

A

N

C

R

G

V

A

T

T

Q

T

V

L

V

M

Y

F

E

E

G

G

T

V

P
|
P

N

N

T

W

P

P

H

T

E

P

G

A

R

R

H

M

W

C

E

Q

I

V

Q

D

K

K

Y

H

K

Q

V

V

S

N

I

I

Y

L

Y

Y

T

T

A
|
A

P

P

T

T

L
x
A

I

I

R

R

T

A

F

L

M

M

K

A

W

E

G

G

N

D

E

K

I

A

P

I

A

E

K

G

Y

T

D

D

L

R

S

S

L

L

R

R

V

I

L

L

G

G

S

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

A

A

W

W

M

E

W

W

Y

Y

R

W

E

K

H

K

I

I

G

G

G

K

N

E

R

K

C

C

P

P

V

V

D

D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

I

F

M

M

I

I

S

T

P

P

L

L

P

P

G

G

V

A

T

I

H

E

A

L

K

K

P

A

G

G

S

S

A

A

Q

T

R

R

P

P

L

F

P

F

G

G

I

V

S

Q

A

P

K

A

V

L

V

V

D

D

D

N

T

E

G

G

T

H

E

P

V

Q

G

E

K

G

G

A

G

T

G

E

G

G

Y

N

L

L

V

V

L

I

D

T

K

D

P

S

W

W

P

P

G

G

M

Q

L

A

R

R

G

T

I

L

W

F

G

G

D

D

D

H

E

E

R

R

Y

F

K

E

E

Q

T

T

Y

Y

W

F

S

S

R

T

F

F

A

-

E

-

Q

K

G

N

F

M

Y

Y

F

F

A

S

G

G

D
|
D

G

G

A

A

K

R

Y

R

D

D

A

E

D

D

G

G

D

Y

I

Y

W

W

L
x
I

L

T

G

G

R
|
R

V

V

D

D

V

V

M

L

N
|
N

V

V

S
|
S

G

G

H

H

R
|
R

I

L

S

G

T

T

T

A

E

E

V

I

E

E

S

S

A

A

L

L

V

V

S

A

H

H

P

P

T

K

V

I

A

A

E

E

A

A

V

V

G

G

A

I

T

P

D

H

P

A

T

I

T

K

G

G

Q

Q

G

A

I

I

V

Y

A

A

F

Y

V

V

I

T

L

L

R

N

G

H

G

G

V

-

A

-

E

-

D

-

E

E

A

E

G

P

G

S

T

P

E

E

A

L

I

Y

Q

A

A

E

L

V

R

R

N

N

H

W

V

V

A
x
R

K

K

E

E

I

I

G

G

P
|
P

I

L

A

A

K

T

P

P

R

D

Q

V

I

L

M

H

V

W

V

T

P

D

E

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

L

I

L

L

R

R

D

K

V

I

A

A

E

A

N

-

R

-

E

-

I

-

G

G

D

D

V

T

-

S

T

S

T

T

L

L

A

A

D

D

S

P

S

G

V

V

M

V

D

E

L

K

I

L

active site: T259 (= T266), T411 (= T419), E412 (= E420), N516 (= N526), R521 (= R531), K604 (= K618)
binding adenosine monophosphate: G382 (= G390), E383 (= E391), P384 (= P392), T407 (= T415), W408 (= W416), W409 (= W417), Q410 (= Q418), T411 (= T419), D495 (= D505), I507 (≠ L517), R510 (= R520), N516 (= N526), R521 (= R531)
binding coenzyme a: F158 (= F167), R186 (= R195), W304 (= W312), T306 (= T314), P329 (= P337), A352 (= A360), A355 (≠ L363), S518 (= S528), R579 (≠ A593), P584 (= P598)

1pg3A Acetyl coa synthetase, acetylated on lys609 (see paper)
51% identity, 93% coverage: 35:646/659 of query aligns to 24:626/634 of 1pg3A

query
sites
1pg3A

Y

Y

A

K

E

Q

A

S

E

I

Q

N

D

D

R

P

E

D

A

T

F

F

W

W

A

G

R

E

Q

Q

A

G

E

K

R

I

L

L

H

D

W

W

D

I

T

T

R

P

W

Y

S

Q

Q

K

V

V

L

K

D

N

W

T

S

S

Q

F

A

A

P

P

-

G

-

N

-

V

F

S

A

I

K

K

W

W

F

Y

V

E

G

D

G

G

K

T

L

L

N

N

V

L

A

A

Y

A

N

N

C

C

V

L

D

D

R

R

H

H

V

L

E

Q

A

E

G

-

H

N

G

G

D

D

Q

R

V

T

A

A

I

I

H

I

W

W

V

E

G

G

E

D

-

D

P

T

G

S

D

Q

T

S

R

K

D

H

I

I

T

S

Y

Y

A

R

Q

E

L

L

K

H

D

R

E

D

V

V

C

C

K

R

A

F

A

A

N

N

A

T

L

L

T

L

S

D

L

L

G

G

V

I

T

K

A

K

N

G

D

D

R

V

V

V

A

A

I

I

Q

Y

L

M

Q

P

M

M

I

V

P

P

E

E

A

A

I

A

V

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

M

V

H

H

S

S

V

V

V

I

F
|
F

G
|
G

G

G

F

F

S

S

P

P

T

E

A

A

L

V

R

A

S

G

R

C

V

I

D

I

D

D

A

S

Q

S

A

S

K

R

V

L

V

V

I

I

T

T

S

A

D

D

G

E

Q

G

Y

V

R
|
R

R

A

G

G

K
x
R

A

S

A

I

P

P

M

L

K

K

A

K

N

N

V

V

D

D

E

D

A

A

L

L

A

K

G

N

A

P