SitesBLAST
Comparing WP_009319351.1 NCBI__GCF_000473955.1:WP_009319351.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 13 hits to proteins with known functional sites (download)
6n2oD 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus with 2-oxoglutarate, coenzyme a and succinyl-coa bound (see paper)
28% identity, 87% coverage: 3:222/253 of query aligns to 2:208/291 of 6n2oD
- binding magnesium ion: D101 (= D103), N129 (= N131), I131 (= I133)
- binding succinyl-coenzyme a: I57 (≠ V59), R62 (≠ F64), L134 (≠ M136), K136 (≠ G138)
- binding iron/sulfur cluster: W24 (≠ Y27), C25 (= C28), C28 (= C31), C59 (= C61), C208 (= C222)
- binding thiamine diphosphate: I57 (≠ V59), G58 (= G60), C59 (= C61), S60 (≠ A62), H76 (= H78), G102 (= G104), D103 (= D105), N129 (= N131), I131 (= I133), G133 (= G135), L134 (≠ M136), T135 (= T137)
Sites not aligning to the query:
6n2oB 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus with 2-oxoglutarate, coenzyme a and succinyl-coa bound (see paper)
28% identity, 87% coverage: 3:222/253 of query aligns to 2:208/291 of 6n2oB
- binding 2-oxoglutaric acid: R62 (≠ F64), L134 (≠ M136)
- binding coenzyme a: K136 (≠ G138), Y150 (≠ A152)
- binding magnesium ion: D101 (= D103), N129 (= N131), I131 (= I133)
- binding iron/sulfur cluster: W24 (≠ Y27), C25 (= C28), C28 (= C31), C59 (= C61), C208 (= C222)
- binding thiamine diphosphate: I57 (≠ V59), G58 (= G60), C59 (= C61), S60 (≠ A62), H76 (= H78), G102 (= G104), D103 (= D105), N129 (= N131), I131 (= I133), Y132 (= Y134), G133 (= G135), L134 (≠ M136), T135 (= T137)
Sites not aligning to the query:
6n2nB Crystal structure of 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus (see paper)
28% identity, 87% coverage: 3:222/253 of query aligns to 2:208/291 of 6n2nB
- binding magnesium ion: D101 (= D103), N129 (= N131), I131 (= I133)
- binding iron/sulfur cluster: W24 (≠ Y27), C25 (= C28), C28 (= C31), H30 (= H33), C59 (= C61), C208 (= C222)
- binding thiamine diphosphate: I57 (≠ V59), G58 (= G60), C59 (= C61), S60 (≠ A62), H76 (= H78), G100 (= G102), D101 (= D103), G102 (= G104), D103 (= D105), N129 (= N131), I131 (= I133), Y132 (= Y134), G133 (= G135), L134 (≠ M136), T135 (= T137)
Sites not aligning to the query:
Q96XT4 2-oxoacid:ferredoxin oxidoreductase 2, subunit beta; OFOR2; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
29% identity, 77% coverage: 27:222/253 of query aligns to 11:197/304 of Q96XT4
- C12 (= C28) binding [4Fe-4S] cluster
- C15 (= C31) binding [4Fe-4S] cluster
- IGCS 44:47 (≠ VGCA 59:62) binding thiamine diphosphate
- C46 (= C61) binding [4Fe-4S] cluster
- D90 (= D103) binding Mg(2+)
- GD 91:92 (= GD 104:105) binding thiamine diphosphate
- N118 (= N131) binding Mg(2+)
- V120 (≠ I133) binding Mg(2+)
- GL 122:123 (≠ GM 135:136) binding thiamine diphosphate
- C197 (= C222) binding [4Fe-4S] cluster
5b46B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - ligand free form (see paper)
29% identity, 77% coverage: 27:222/253 of query aligns to 8:194/301 of 5b46B
- binding magnesium ion: D87 (= D103), N115 (= N131), V117 (≠ I133)
- binding iron/sulfur cluster: W8 (≠ Y27), C9 (= C28), C12 (= C31), C43 (= C61), C194 (= C222)
- binding thiamine diphosphate: I41 (≠ V59), G42 (= G60), C43 (= C61), S44 (≠ A62), H62 (= H78), G86 (= G102), G88 (= G104), D89 (= D105), N115 (= N131), V117 (≠ I133), Y118 (= Y134), G119 (= G135), L120 (≠ M136), T121 (= T137)
Sites not aligning to the query:
5b47B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - pyruvate complex (see paper)
25% identity, 88% coverage: 27:249/253 of query aligns to 4:219/275 of 5b47B
- binding magnesium ion: D83 (= D103), N111 (= N131)
- binding iron/sulfur cluster: C5 (= C28), C8 (= C31), C39 (= C61), C179 (= C222)
- binding thiamine diphosphate: I37 (≠ V59), G38 (= G60), C39 (= C61), S40 (≠ A62), H58 (= H78), G84 (= G104), D85 (= D105), N111 (= N131), V113 (≠ I133), Y114 (= Y134), L116 (≠ M136)
Q96Y68 2-oxoacid:ferredoxin oxidoreductase 1, subunit beta; OFOR1; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 2 papers)
28% identity, 77% coverage: 27:222/253 of query aligns to 11:197/305 of Q96Y68
- C12 (= C28) binding [4Fe-4S] cluster
- C15 (= C31) binding [4Fe-4S] cluster
- C46 (= C61) binding [4Fe-4S] cluster
- K49 (vs. gap) mutation to I: Loss of oxidoreductase activity toward 2-oxoglutarate but retains its activity toward pyruvate.
- D90 (= D103) binding Mg(2+)
- GD 91:92 (= GD 104:105) binding thiamine diphosphate
- N118 (= N131) binding Mg(2+)
- V120 (≠ I133) binding Mg(2+)
- GL 122:123 (≠ GM 135:136) binding thiamine diphosphate
- K125 (≠ G138) Plays an important role in the binding of CoA; mutation to A: Shows a strong decrease of affinity for CoA and a poor inactivation by 4-fluoro-7-nitrobenzofurazan (NBD-F).
- K173 (= K198) mutation to A: Same oxidoreductase activity as the wild-type.
- C197 (= C222) binding [4Fe-4S] cluster
P72579 2-oxoacid:ferredoxin oxidoreductase subunit beta; OFOR; EC 1.2.7.11 from Sulfolobus sp. (see paper)
28% identity, 77% coverage: 27:222/253 of query aligns to 11:197/305 of P72579
- K49 (vs. gap) mutation to I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to R: Increase the oxidoreductase activity with pyruvate.; mutation to V: Slight decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.
- L123 (≠ M136) mutation L->A,I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to N: Strong decrease of the oxidoreductase activity with pyruvate and 2-oxobutyrate. However, this mutant shows almost the same activity with 2-oxoglutarate as the wild-type.
5b48B 2-oxoacid:ferredoxin oxidoreductase 1 from sulfolobus tokodai (see paper)
28% identity, 77% coverage: 27:222/253 of query aligns to 7:183/286 of 5b48B
- binding magnesium ion: D84 (= D103), N112 (= N131), V114 (≠ I133)
- binding iron/sulfur cluster: C8 (= C28), C11 (= C31), C42 (= C61), C183 (= C222)
- binding 2-[(2E)-3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-(1-oxidanylpropylidene)-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: I40 (≠ V59), G41 (= G60), C42 (= C61), S43 (≠ A62), H59 (= H78), G85 (= G104), D86 (= D105), N112 (= N131), V114 (≠ I133), Y115 (= Y134), G116 (= G135), L117 (≠ M136)
Sites not aligning to the query:
9bt4C Pyruvate:ferredoxin oxidoreductase from methanosarcina acetivorans (see paper)
24% identity, 81% coverage: 23:226/253 of query aligns to 7:210/291 of 9bt4C
- binding magnesium ion: D94 (= D103), N122 (= N131), A124 (≠ I133)
- binding iron/sulfur cluster: C13 (= C28), C16 (= C31), C17 (≠ S32), D18 (≠ H33), C41 (= C61), M126 (≠ G135), C206 (= C222), T208 (≠ S224)
- binding thiamine diphosphate: D18 (≠ H33), T39 (≠ V59), G40 (= G60), C41 (= C61), F63 (≠ H78), E64 (≠ G79), G93 (= G102), D94 (= D103), G95 (= G104), S96 (≠ D105), N122 (= N131), A124 (≠ I133), Y125 (= Y134), M126 (≠ G135), N127 (≠ M136), T128 (= T137)
5exdF Crystal structure of oxalate oxidoreductase from moorella thermoacetica bound with carboxy-di-oxido-methyl-tpp (coom-tpp) intermediate (see paper)
23% identity, 86% coverage: 28:245/253 of query aligns to 24:252/310 of 5exdF
- active site: N143 (≠ M136)
- binding magnesium ion: D110 (= D103), N138 (= N131), S140 (≠ I133)
- binding [2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-[1,1,2-tris(oxidanyl)-2-oxidanylidene-ethyl]-1,3-thiazol-3-ium-5-yl]ethoxy-oxidanyl-phosphoryl] hydrogen phosphate: T50 (≠ V59), G51 (= G60), C52 (= C61), I74 (≠ H78), D110 (= D103), G111 (= G104), Y136 (≠ I129), N138 (= N131), S140 (≠ I133), Y141 (= Y134), A142 (≠ G135), N143 (≠ M136), T144 (= T137)
- binding iron/sulfur cluster: C24 (= C28), C27 (= C31), P29 (≠ H33), C52 (= C61), A142 (≠ G135), C225 (= C222), K227 (≠ S224)
5c4iC Structure of an oxalate oxidoreductase (see paper)
23% identity, 86% coverage: 28:245/253 of query aligns to 24:252/312 of 5c4iC
- active site: N143 (≠ M136)
- binding magnesium ion: D110 (= D103), N138 (= N131), S140 (≠ I133)
- binding iron/sulfur cluster: C24 (= C28), C27 (= C31), P29 (≠ H33), C52 (= C61), A142 (≠ G135), C225 (= C222), P226 (≠ N223), K227 (≠ S224)
- binding thiamine diphosphate: T50 (≠ V59), G51 (= G60), C52 (= C61), I74 (≠ H78), G109 (= G102), D110 (= D103), G111 (= G104), Y136 (≠ I129), N138 (= N131), S140 (≠ I133), Y141 (= Y134), A142 (≠ G135), N143 (≠ M136), T144 (= T137)
5exeC Crystal structure of oxalate oxidoreductase from moorella thermoacetica bound with carboxy-tpp adduct (see paper)
23% identity, 86% coverage: 28:245/253 of query aligns to 24:252/314 of 5exeC
- active site: N143 (≠ M136)
- binding [2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-carboxy-4-methyl-1,3-thiazol-3-ium-5-yl]ethoxy-oxidanyl-phosphoryl] hydrogen phosphate: T50 (≠ V59), G51 (= G60), C52 (= C61), I74 (≠ H78), G109 (= G102), D110 (= D103), G111 (= G104), Y136 (≠ I129), N138 (= N131), S140 (≠ I133), Y141 (= Y134), A142 (≠ G135), N143 (≠ M136), T144 (= T137)
- binding magnesium ion: D110 (= D103), D130 (≠ N123), N138 (= N131), S140 (≠ I133), L211 (= L200), Q213 (= Q202)
- binding iron/sulfur cluster: C24 (= C28), C27 (= C31), P29 (≠ H33), C52 (= C61), C225 (= C222), P226 (≠ N223), K227 (≠ S224)
Query Sequence
>WP_009319351.1 NCBI__GCF_000473955.1:WP_009319351.1
MDVNEIIKPENLVYDKPRLMNDNPMHYCPGCSHGVVHKLVAEVIDEMGMEEQAIGIAPVG
CAVFAYNYIDIDWLEAAHGRAPAIATAVKRLNPSKMVFTYQGDGDLAAIGTAETIHACNR
GENIVIIFINNAIYGMTGGQMAPTTLEGMVTATCPYGRNVELNGYPLKISNLVAQLDGTC
YVTRQSVHTAAAVKKAKKALRQAFENSMARKGTSLVEFVSTCNSGWKMTPDQANKWMEQN
MFAKYPLGDLKNE
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory