SitesBLAST

P17597 Acetolactate synthase, chloroplastic; AtALS; Acetohydroxy-acid synthase; Protein CHLORSULFURON RESISTANT 1; EC 2.2.1.6 from Arabidopsis thaliana (Mouse-ear cress) (see 8 papers)
45% identity, 91% coverage: 17:581/619 of query aligns to 95:661/670 of P17597

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A122 (= A44) mutation to V: Reduced catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
M124 (≠ L46) mutation to E: Reduced catalytic activity. Resistant to imidazolinone herbicides and reduced sensitivity to sulfonylurea herbicides.; mutation to I: No effect on catalytic activity. Increased resistance to imidazolinone herbicides.
E144 (= E69) binding thiamine diphosphate
S186 (= S111) binding FAD
P197 (≠ G122) mutation to S: In csr1-1/GH50; resistant to sulfonylurea but not to imidazolinone herbicides.
R199 (≠ A124) mutation R->A,E: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
Q207 (= Q132) binding thiamine diphosphate
K220 (= K145) binding (R)-imazaquin
R246 (= R171) binding (R)-imazaquin; binding FAD
K256 (= K181) binding chlorimuron-ethyl
G308 (= G233) binding FAD
TL 331:332 (= TL 258:259) binding FAD
C340 (≠ E267) modified: Cysteine sulfinic acid (-SO2H)
LGMH 349:352 (= LGMH 276:279) binding FAD
GVRFD 371:375 (≠ GARFD 298:302) binding FAD
DR 376:377 (= DR 303:304) binding chlorimuron-ethyl
DI 395:396 (= DI 322:323) binding FAD
DV 414:415 (= DV 341:342) binding FAD
QH 487:488 (= QH 414:415) binding thiamine diphosphate
GG 508:509 (≠ AG 434:435) binding FAD
GAM 511:513 (≠ GTM 437:439) binding thiamine diphosphate
D538 (= D464) binding Mg(2+)
DGS 538:540 (≠ DAS 464:466) binding thiamine diphosphate
N565 (= N491) binding Mg(2+)
NQHLGM 565:570 (≠ NGWQGM 491:496) binding thiamine diphosphate
H567 (≠ W493) binding Mg(2+)
W574 (= W500) binding chlorimuron-ethyl; mutation to L: Increased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.; mutation to S: Slightly decreased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.
S653 (≠ P573) binding chlorimuron-ethyl; mutation to A: No effect on catalytic activity or sensitivity to herbicides.; mutation to F: No effect on catalytic activity. Resistant to imidazolinone herbicides and also slightly sulfonylurea-resistant.; mutation to N: In csr1-2/GH90; no effect on catalytic activity. Resistant to imidazolinone but not to sulfonylurea herbicides.; mutation to T: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.

7tzzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase p197t mutant in complex with bispyribac-sodium (see paper)
45% identity, 91% coverage: 17:581/619 of query aligns to 10:576/582 of 7tzzA

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binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: M266 (= M278), R292 (= R304), W489 (= W500), S568 (≠ P573)
binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V412), G401 (= G413), Q402 (= Q414), H403 (= H415), G426 (= G437), M428 (= M439), G452 (= G463), D453 (= D464), G454 (≠ A465), S455 (= S466), L483 (≠ Q494), G484 (= G495), M485 (= M496), V486 (= V497)
binding flavin-adenine dinucleotide: R161 (= R171), G222 (= G232), G223 (= G233), G224 (= G234), T246 (= T258), L247 (= L259), M248 (= M260), M263 (= M275), L264 (= L276), M266 (= M278), H267 (= H279), G286 (= G298), R288 (= R300), V293 (= V305), D310 (= D322), I311 (= I323), D329 (= D341), V330 (= V342), M405 (= M417), G423 (≠ A434)
binding magnesium ion: A37 (= A44), T82 (= T92), S83 (= S93), Q122 (= Q132), Y381 (≠ E394), D453 (= D464), M458 (= M469), Q461 (= Q472), N480 (= N491), H482 (≠ W493), K533 (≠ H538)

5k3sA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, bispyribac-sodium (see paper)
45% identity, 91% coverage: 17:581/619 of query aligns to 10:576/583 of 5k3sA

query
sites
5k3sA

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active site: Y33 (= Y40), G35 (= G42), G36 (= G43), A37 (= A44), S38 (≠ I45), E59 (= E69), T82 (= T92), F121 (= F131), Q122 (= Q132), E123 (= E133), K171 (= K181), M266 (= M278), V293 (= V305), V400 (= V412), G426 (= G437), M428 (= M439), D453 (= D464), N480 (= N491), H482 (≠ W493), L483 (≠ Q494), M485 (= M496), V486 (= V497), W489 (= W500), H558 (≠ R563)
binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: R292 (= R304), M485 (= M496), W489 (= W500), G569 (= G574)
binding flavin-adenine dinucleotide: R161 (= R171), G222 (= G232), G223 (= G233), G224 (= G234), T246 (= T258), L247 (= L259), M248 (= M260), L264 (= L276), M266 (= M278), G286 (= G298), R288 (= R300), D290 (= D302), V293 (= V305), D310 (= D322), I311 (= I323), D329 (= D341), V330 (= V342), M405 (= M417), G423 (≠ A434)
binding magnesium ion: D453 (= D464), N480 (= N491), H482 (≠ W493)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V412), G401 (= G413), Q402 (= Q414), H403 (= H415), G426 (= G437), M428 (= M439), D453 (= D464), G454 (≠ A465), S455 (= S466), N480 (= N491), H482 (≠ W493), L483 (≠ Q494), G484 (= G495), M485 (= M496), V486 (= V497)

5k2oA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, pyrithiobac (see paper)
45% identity, 91% coverage: 17:581/619 of query aligns to 10:576/585 of 5k2oA

query
sites
5k2oA

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I

I

active site: Y33 (= Y40), G35 (= G42), G36 (= G43), A37 (= A44), S38 (≠ I45), E59 (= E69), T82 (= T92), F121 (= F131), Q122 (= Q132), E123 (= E133), K171 (= K181), M266 (= M278), V293 (= V305), V400 (= V412), G426 (= G437), M428 (= M439), D453 (= D464), N480 (= N491), H482 (≠ W493), L483 (≠ Q494), M485 (= M496), V486 (= V497), W489 (= W500), H558 (≠ R563)
binding 2-chloranyl-6-(4,6-dimethoxypyrimidin-2-yl)sulfanyl-benzoic acid: M266 (= M278), R292 (= R304), W489 (= W500), S568 (≠ P573)
binding flavin-adenine dinucleotide: R161 (= R171), G222 (= G232), G223 (= G233), G224 (= G234), T246 (= T258), L247 (= L259), M248 (= M260), L264 (= L276), G286 (= G298), R288 (= R300), D290 (= D302), V293 (= V305), D310 (= D322), I311 (= I323), D329 (= D341), V330 (= V342), Q404 (= Q416), M405 (= M417), G423 (≠ A434)
binding magnesium ion: D453 (= D464), N480 (= N491), H482 (≠ W493)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V412), G401 (= G413), Q402 (= Q414), H403 (= H415), M428 (= M439), D453 (= D464), G454 (≠ A465), S455 (= S466), N480 (= N491), H482 (≠ W493), L483 (≠ Q494), G484 (= G495), M485 (= M496), V486 (= V497)

8et4A Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with amidosulfuron (see paper)
45% identity, 91% coverage: 17:581/619 of query aligns to 10:576/582 of 8et4A

query
sites
8et4A

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