SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_009543700.1 NCBI__GCF_000017845.1:WP_009543700.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4rjkG Acetolactate synthase from bacillus subtilis bound to lthdp - crystal form ii (see paper)
36% identity, 97% coverage: 7:538/550 of query aligns to 4:540/553 of 4rjkG

query
sites
4rjkG

A

A

E

E

L

L

L

V

V

V

K

D

C

C

L

L

E

V

N

E

E

Q

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G

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M

A

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G

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T

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L

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N

D
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N

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x
T

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G
x
D

L
x
M

I
x
V

E

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W

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D

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L

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A

binding magnesium ion: D437 (= D435), D464 (= D462), T466 (≠ G464)
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-carboxy-1-hydroxyethyl)-5-(2-{[hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: E47 (= E50), Q110 (= Q113)
binding thiamine diphosphate: I384 (≠ V382), G385 (= G383), S386 (≠ A384), H387 (= H385), Q410 (≠ A408), L412 (≠ M410), G436 (= G434), D437 (= D435), G438 (= G436), G439 (= G437), T466 (≠ G464), Y467 (= Y465), D468 (≠ G466), M469 (≠ L467), V470 (≠ I468), Y533 (= Y531)

4rjkF Acetolactate synthase from bacillus subtilis bound to lthdp - crystal form ii (see paper)
36% identity, 97% coverage: 7:538/550 of query aligns to 4:540/552 of 4rjkF

query
sites
4rjkF

A

A

E

E

L

L

L

V

V

V

K

D

C

C

L

L

E

V

N

E

E

Q

G

G

V

V

E

T

Y

H

I

V

F

F

G

G

L

I

P
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P

G

G

E
x
A

E
x
K

N

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L

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H

A

I

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L

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E

D

A

A

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Q

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D

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K

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P

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E

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I

I

I

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T

A

R

R

H

H

E
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E

Q

Q

G

N

A

A

F

F

M

M

A

A

D

Q

V

A

Y

V

G

G

R

R

L

L

T

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G

G

K

K

A

P

G

G

V

V

C

V

L

L

S

V

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T

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S

G

G

P
|
P

G

G

A

A

T

S

N

N

L

L

M

A

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T

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G

V

L

A

L

D

T

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A

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N

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A

binding magnesium ion: D437 (= D435), D464 (= D462), T466 (≠ G464)
binding pyruvic acid: A25 (≠ E28), K26 (≠ E29)
binding thiamine diphosphate: P23 (= P26), E47 (= E50), P73 (= P76), G385 (= G383), S386 (≠ A384), H387 (= H385), Q410 (≠ A408), L412 (≠ M410), G436 (= G434), D437 (= D435), G438 (= G436), G439 (= G437), T466 (≠ G464), Y467 (= Y465), D468 (≠ G466), M469 (≠ L467), V470 (≠ I468), Y533 (= Y531)

4rjiC Acetolactate synthase from bacillus subtilis bound to thdp - crystal form i (see paper)
36% identity, 97% coverage: 7:538/550 of query aligns to 5:541/555 of 4rjiC

query
sites
4rjiC

A

A

E

E

L

L

L

V

V

V

K

D

C

C

L

L

E

V

N

E

E

Q

G

G

V

V

E

T

Y

H

I

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F

F

G

G

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P
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P

G

G

E

A

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K

N

I

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A

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F

E

D

A

A

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D

D

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F

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I

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R

H

H

E
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Q

Q

G

N

A

A

F

F

M

M

A

A

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A

Y

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K

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C

V

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L

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T

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G

G

P
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P

G

G

A

A

T

S

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N

L

L

M

A

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T

G

G

V

L

A

L

D

T

A

A

N

N

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P

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A

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K

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V

S

K

I

N

T

I

P

P

E

E

V

A

T

V

R

T

K

N

A

A

F

F

K

R

L

I

A

A

Q

S

S

A

E

G

K

Q

P

A

G

G

A

A

V

A

H

F

I

V

D

S

L

F

P

P

E

Q

N

D

I

V

A

V

A

N

L

-

P

-

V

-

E

-

G

-

W

-

P

E

L

V

T

T

R

N

D

T

S

K

Q

N

E

V

K

R

T

A

Y

V

A

A

S

A

Y

P

R

K

-

L

-

G

-

P

-

A

-

D

S

A

L

I

N

S

A

A

A

I

M

A

A

K

I

I

S

Q

K

T

A

A

K

K

N

L

P

P

L

V

I

V

L

L

V

V

G

G

N

M

G

K

T

G

I

G

R

R

A

P

H

E

A

A

S

I

E

K

A

A

L

V

T

R

E

K

F

L

A

L

T

K

L

V

N

Q

I

L

P

P

V

F

A

V

N

E

T

T

F

Y

M

Q

G

A

K

A

G

G

A

T

V

L

P

S

F

R

T

D

-

L

H

E

P

D

L

Q

S

Y

L

F

W

G

T

R

V

I

G

G

L

L

Q

F

Q

R

R

N

D

Q

L

P

I

G

T

D

C

L

A

L

F

L

E

E

Q

Q

S

A

D

D

L

V

V

V

I

L

A

T

V

I

G

G

Y

Y

D

D

L

P

I

I

E

E

Y

Y

S

D

P

P

K

K

K

F

W

W

N

N

P

I

E

N

G

G

T

D

T

R

P

T

I

I

H

H

I

L

G

D

M

E

T

I

P

I

A

A

E

D

I

I

D

D

S

H

S

A

Y

Y

I

Q

P

P

L

D

V

L

E

E

V

L

V

I

G

G

D

D

I

I

S

P

D

S

S

T

L

I

M

N

D

H

I

I

-

E

-

H

-

D

-

A

-

V

-

K

L

V

K

E

R

F

A

A

D

E

R

R

Q

E

G

Q

K

K

S

-

T

-

S

-

V

-

A

-

G

-

L

I

R

L

S

S

D

D

L

L

V

-

R

-

E

-

Y

-

E

K

K

Q

Y

Y

A

M

H

H

D

E

-

G

E

E

G

Q

F

V

P

P

V

A

-

D

-

W

-

K

-

S

-

D

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R

-

A

K

H

P

P

Q

L

K

E

I

I

I

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Y

K

D

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L

L

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R

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A

M

V

G

D

P

D

E

H

D

V

I

T

V

V

I

T

S

C

D

D

V

I

G

G

A
x
S

H
|
H

K

A

M

I

W

W

M

M

A

S

R

R

H

Y

Y

F

H

R

C

S

D

Y

T

E

P

P

N

L

T

T

C

L

L

M

I

I

S

S

N

N

G

G

F

M

A
x
Q

A

T

M

L

G

G

I

V

A

A

I

L

P

P

G

W

A

A

I

I

A

G

A

A

K

S

L

L

V

V

A

K

P

P

D

G

K

E

K

K

V

V

A

S

V

V

T

S

G
|
G

D
|
D

G
|
G

F

F

M

L

M

F

N

S

C

A

Q

M

E

E

L

L

E

E

T

T

A

A

L

V

R

R

A

L

G

K

T

A

P

P

F

I

V

V

T

H

L

I

I

V

F

W

N

N

D
|
D

N

S

G
x
T

Y
|
Y

G
x
D

L
x
M

I
x
V

E

A

W

F

K

Q

Q

Q

I

L

N

K

Q

K

F

Y

G

N

Q

R

S

T

S

S

F

A

I

V

K

D

F

F

T

G

N

N

P

I

D

D

F

I

V

V

K

K

F

Y

A

A

E

E

S

S

M

F

G

G

L

A

K

T

G

G

Y

L

R

R

V

V

E

E

S

S

A

P

D

D

D

Q

L

L

V

A

P

D

T

V

L

L

K

R

T

Q

A

G

L

M

E

N

Q

A

G

E

V

G

P

P

A

V

V

I

I

I

D

D

C

V

P

P

V

V

D

D

Y
|
Y

Q

S

E

D

N

N

I

I

R

N

F

L

S

A

binding magnesium ion: D438 (= D435), D465 (= D462), T467 (≠ G464)
binding thiamine diphosphate: P24 (= P26), E48 (= E50), P74 (= P76), S387 (≠ A384), H388 (= H385), Q411 (≠ A408), G437 (= G434), D438 (= D435), G439 (= G436), G440 (= G437), T467 (≠ G464), Y468 (= Y465), D469 (≠ G466), M470 (≠ L467), V471 (≠ I468), Y534 (= Y531)

5d6rB Acetolactate synthase from klebsiella pneumoniae in complex with mechanism-based inhibitor
39% identity, 96% coverage: 7:534/550 of query aligns to 8:538/548 of 5d6rB

query
sites
5d6rB

A

A

E

D

L

L

L

V

V

V

K

S

C

Q

L

L

E

E

N

A

E

Q

G

G

V

V

E

R

Y

Q

I

V

F

F

G

G

L
x
I

P

P

G
|
G

E
x
A

E
x
K

N
x
I

L

D

H

K

I

V

L

F

E

D

A

S

L

L

K

L

D

D

S

S

I

I

Q

R

F

I

I

I

T

P

T

V

R

R

H

H

E
|
E

Q

A

G

N

A

A

F

F

M

M

A

A

D

A

V

A

Y

V

G

G

R
|
R

L

I

T

T

G

G

K

K

A

A

G

G

V

V

C

A

L

L

S

V

T
|
T

L

S

G

G

P

P

G

G

A

C

T

S

N

N

L

L

M

I

T

T

G

G

V

M

A

A

D

T

A

A

N

N

L

S

D

E

G

G

A

D

P

P

L

V

V

V

A

A

I

L

T

G

G

G

Q

A

V

V

G

K

T

R

D

A

R

D

M

K

H

A

I

K

E

Q

S

V

H
|
H

Q
|
Q

Y
x
S

L

M

D

D

L

T

V

V

A

A

M

M

F

F

A

S

P

P

V

V

T

T

K

K

W

Y

N

A

K

I

Q

E

I

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V

T

R

A

P

P

S

D

I

A

T

L

P

A

E

E

V

V

T

V

R

S

K

N

A

A

F

F

K

R

L

A

A

A

Q

E

S

Q

E

G

K

R

P

P

G

G

A

S

V

A

H

F

I

V

D

S

L

L

P

P

E
x
Q

N

D

I

V

A

V

A

D

L

G

P

P

V

V

E

S

G

G

W

K

P

V

L

L

T

P

R

A

D

S

S

P

Q

Q

E

M

K

G

T

A

Y

-

A

A

S

P

Y

D

R

D

S

A

L

I

N

D

A

Q

A

V

A

A

M

K

A

L

I

I

S

A

K

Q

A

A

K

K

N

N

P

P

L

I

I

F

L

L

V

L

G

G

N

L

G

M

T

A

I

S

R
x
Q

A

P

H

E

A

N

S

S

E

K

A

A

L

L

T

R

E

R

F

L

A

L

T

E

T

T

L

S

N

H

I

I

P

P

V

V

A

T

N

S

T

T

F

Y

M

Q

G

A

K

A

G

G

A

A

V

V

P

N

F

Q

T

D

H

N

P

-

L

F

S

S

L

R

W

F

T

A

-

G

-

R

V

V

G

G

L
|
L

Q

F

Q

N

R

N

D

Q

L

A

I

G

T

D

C

R

A

L

F

L

E

Q

Q

L

S

A

D

D

L

L

V

V

I

I

A

C

V

I

G

G

Y

Y

D

S

L

P

I

V

E

E

Y

Y

S
x
E

P

P

K

A

K

M

W

W

N

N

P

-

E

S

G

G

T

N

T

A

P

T

I

L

I

V

H

H

I

I

G

D

M

V

T

L

P

P

A

A

E

Y

I

E

D

E

S

R

S

N

Y

Y

I

T

P

P

L

D

V

V

E

E

V

L

V

V

G

G

D

D

I

I

S

A

D

G

S

T

L

L

M

N

D

K

I

L

L

A

K

Q

R

N

A

I

D

D

R

H

Q

R

G

L

K

V

S

L

T

S

S

P

V

Q

A

A

G

E

L

I

R

L

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D

D

L

-

V

-

R

R

E

Q

Y

H

E

Q

K

R

Y

E

A

L

H

L

D

D

E

R

G

R

F

G

P

A

V

Q

K

L

P

N

Q

Q

K

F

I

A

I

L

Y

H

D

P

L

L

R

R

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I

V

V

M

R

G

A

P

M

E

Q

D

D

I

I

V

V

I

N

S

S

D

D

V

V

-

T

-

L

-

T

-

V

-

D

-
x
M

G
|
G

A
x
S

H

F

K

H

M

I

W

W

M

I

A

A

R

R

H

Y

Y

L

H

Y

C

S

D

F

T

R

P

A

N

R

T

Q

C

V

L

M

I

I

S

S

N

N

G

G

F

Q

A
x
Q

A

T

M
|
M

G

G

I

V

A

A

I

L

P

P

G

W

A

A

I

I

A

G

A

A

K

W

L

L

V

V

A

N

P

P

D

E

K

R

K

K

V

V

A

S

V

V

T

S

G

G

D
|
D

G
|
G

G

G

F

F

M

L

M

Q

N

S

C

S

Q

M

E

E

L

L

E

E

T

T

A

A

L

V

R

R

A

L

G

K

T

A

P

N

F

V

V

L

T

H

L

L

I

I

F

W

N

V

D
|
D

N

N

G
|
G

Y
|
Y

G
x
N

L
x
M

I
x
V

E

A

W

I

K
x
Q

Q

E

I

E

N

K

Q

K

F

Y

G

Q

Q

R

S

L

S

S

F

G

I

V

K

E

F

F

T

G

N

P

P

M

D

D

F

F

V

K

K

A

F

Y

A

A

E

E

S

S

M

F

G

G

L

A

K

K

G

G

Y

F

R

A

V

V

E

E

S

S

A

A

D

E

D

A

L

L

V

E

P

P

T

T

L

L

K

R

T

A

A

A

L

M

E

D

Q

V

G

D

V

G

P

P

A

A

V

V

I

V

D

A

C

I

P

P

V

V

D

D

Y
|
Y

Q

R

E

D

N

N

active site: I26 (≠ L25), G28 (= G27), A29 (≠ E28), K30 (≠ E29), I31 (≠ N30), E51 (= E50), T74 (= T73), H113 (= H112), Q114 (= Q113), S115 (≠ Y114), Q163 (≠ E162), L254 (= L253), E281 (≠ S280), M386 (vs. gap), Q412 (≠ A408), M414 (= M410), D439 (= D435), D466 (= D462), G468 (= G464), Y469 (= Y465), M471 (≠ L467), V472 (≠ I468), Q475 (≠ K471), Y535 (= Y531)
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-[(Z)-2-fluoro-1-hydroxy-2-phosphonoethenyl]-5-(2-{[(S)-hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: M386 (vs. gap), G387 (= G383), S388 (≠ A384), Q412 (≠ A408), M414 (= M410), D439 (= D435), G440 (= G436), G468 (= G464), Y469 (= Y465), N470 (≠ G466), M471 (≠ L467), Y535 (= Y531)
binding magnesium ion: R63 (= R62), Q212 (≠ R212), D439 (= D435), D466 (= D462), G468 (= G464)

5dx6B Acetolactate synthase from klebsiella pneumoniae soaked with beta- fluoropyruvate
38% identity, 96% coverage: 7:534/550 of query aligns to 20:544/557 of 5dx6B

query
sites
5dx6B

A

A

E

D

L

L

L

V

V

V

K

S

C

Q

L

L

E

E

N

A

E

Q

G

G

V

V

E

R

Y

Q

I

V

F

F

G

G

L
x
I

P

P

G
|
G

E
x
A

E
x
K

N
x
I

L

D

H

K

I

V

L

F

E

D

A

S

L

L

K

L

D

D

S

S

I

I

Q

R

F

I

I

I

T

P

T

V

R

R

H

H

E
|
E

Q

A

G

N

A

A

F

F

M

M

A

A

D

A

V

A

Y

V

G

G

R

R

L

I

T

T

G

G

K

K

A

A

G

G

V

V

C

A

L

L

S

V

T
|
T

L

S

G

G

P

P

G

G

A

C

T

S

N

N

L

L

M

I

T

T

G

G

V

M

A

A

D

T

A

A

N

N

L

S

D

E

G

G

A

D

P

P

L

V

V

V

A

A

I

L

T

G

G

G

Q

A

V

V

G

K

T

R

D

A

R

D

M

K

H

A

I

K

E

Q

S

V

H
|
H

Q
|
Q

Y
x
S

L

M

D

D

L

T

V

V

A

A

M

M

F

F

A
x
S

P

P

V

V

T
|
T

K

K

W

Y

N

A

K

I

Q

E

I

V

V

T

R

A

P

P

S

D

I

A

T

L

P

A

E

E

V

V

T

V

R

S

K

N

A

A

F

F

K

R

L

A

A

A

Q

E

S

Q

E

G

K

R

P

P

G

G

A

S

V

A

H

F

I

V

D

S

L

L

P

P

E
x
Q

N

D

I

V

A

V

A

D

L

G

P

P

V

V

E

S

G

G

W

K

P

V

L

L

-

P

T

A

R

S

D

G

S

A

Q

P

E

Q

K

M

T

G

Y

A

A

A

S

P

Y

D

R

D

S

A

L

I

N

D

A

Q

A

V

A

A

M

K

A

L

I

I

S

A

K

Q

A

A

K

K

N

N

P

P

L

I

I

F

L

L

V

L

G

G

N

L

G

M

T

A

I

S

R

Q

A

P

H

E

A

N

S

S

E

K

A

A

L

L

T

R

E

R

F

L

A

L

T

E

T

T

L

S

N

H

I

I

P

P

V

V

A

T

N

S

T

T

F

Y

M

Q

G

A

K

A

G

G

A

A

V

V

P

N

F

Q

T

D

H

N

P

-

L

F

S

S

L

R

W

F

T

A

-
x
G

-
x
R

V

V

G

G

L
|
L

Q

F

Q

N

R

N

D
x
Q

L

A

I

G

T

D

C

R

A

L

F

L

E

Q

Q

L

S

A

D

D

L

L

V

V

I

I

A

C

V

I

G

G

Y

Y

D

S

L

P

I

V

E

E

Y

Y

S
x
E

P

P

K

A

K

M

W

W

N

N

P

-

E

S

G

G

T

N

T

A

P

T

I

L

I

V

H

H

I

I

G

D

M

V

T

L

P

P

A

A

E

Y

I

E

D

E

S

R

S

N

Y

Y

I

T

P

P

L

D

V

V

E

E

V

L

V

V

G

G

D

D

I

I

S

A

D

G

S

T

L

L

M

N

D

K

I

L

L

A

K

Q

R

N

A

I

D

D

R

H

Q

R

G

L

K

V

S

L

T

S

S

P

V

Q

A

A

G

E

L

I

R

L

S

R

D

D

L

-

V

-

R

R

E

Q

Y

H

E

Q

K

R

Y

E

A

L

H

N

D

Q

E

-

G

-

F

F

P

A

V

L

K

H

P

P

Q

L

K

R

I

I

I

V

Y

R

D

A

L

M

R

Q

Q

D

V

I

M

V

G

N

P

S

E

D

D

V

I

T

V

L

I

T

S

V

D

D

V
x
M

G
|
G

A
x
S

H
x
F

K

H

M

I

W

W

M

I

A
|
A

R
|
R

H

Y

Y

L

H
x
Y

C

S

D

F

T

R

P

A

N

R

T

Q

C

V

L

M

I

I

S

S

N

N

G

G

F

Q

A
x
Q

A

T

M
|
M

G

G

I

V

A

A

I

L

P

P

G

W

A

A

I

I

A

G

A

A

K

W

L

L

V

V

A

N

P

P

D

E

K

R

K

K

V

V

A

S

V

V

T

S

G
|
G

D
|
D

G
|
G

G

G

F

F

M

L

M

Q

N

S

C

S

Q

M

E

E

L

L

E

E

T

T

A

A

L

V

R

R

A

L

G

K

T

A

P

N

F

V

V

L

T

H

L

L

I

I

F

W

N

V

D
|
D

N

N

G
|
G

Y
|
Y

G
x
N

L
x
M

I
x
V

E

A

W

I

K
x
Q

Q

E

I

E

N

K

Q

K

F

Y

G

Q

Q

R

S

L

S

S

F

G

I

V

K

E

F

F

T

G

N

P

P

M

D

D

F

F

V

K

K

A

F

Y

A

A

E

E

S

S

M

F

G

G

L

A

K

K

G

G

Y

F

R

A

V

V

E

E

S

S

A

A

D

E

D

A

L

L

V

E

P

P

T

T

L

L

K

R

T

A

A

A

L

M

E

D

Q

V

G

D

V

G

P

P

A

A

V

V

I

V

D

A

C

I

P

P

V

V

D

D

Y
|
Y

Q

R

E

D

N

N

active site: I38 (≠ L25), G40 (= G27), A41 (≠ E28), K42 (≠ E29), I43 (≠ N30), E63 (= E50), T86 (= T73), H125 (= H112), Q126 (= Q113), S127 (≠ Y114), Q175 (≠ E162), L268 (= L253), E295 (≠ S280), M392 (≠ V382), Q418 (≠ A408), M420 (= M410), D445 (= D435), D472 (= D462), G474 (= G464), Y475 (= Y465), M477 (≠ L467), V478 (≠ I468), Q481 (≠ K471), Y541 (= Y531)
binding 3-fluoro-2-oxopropanoic acid: G264 (vs. gap), R265 (vs. gap), Q272 (≠ D257), A400 (= A390), R401 (= R391), Y404 (≠ H394)
binding magnesium ion: S135 (≠ A122), T138 (= T125), D445 (= D435), D472 (= D462), G474 (= G464)
binding thiamine diphosphate: G393 (= G383), S394 (≠ A384), F395 (≠ H385), Q418 (≠ A408), M420 (= M410), G444 (= G434), D445 (= D435), G446 (= G436), D472 (= D462), G474 (= G464), Y475 (= Y465), N476 (≠ G466), M477 (≠ L467), V478 (≠ I468), Y541 (= Y531)

1ozfA The crystal structure of klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactors (see paper)
39% identity, 96% coverage: 7:534/550 of query aligns to 8:537/545 of 1ozfA

query
sites
1ozfA

A

A

E

D

L

L

L

V

V

V

K

S

C

Q

L

L

E

E

N

A

E

Q

G

G

V

V

E

R

Y

Q

I

V

F

F

G

G

L
x
I

P

P

G
|
G

E
x
A

E
x
K

N
x
I

L

D

H

K

I

V

L

F

E

D

A

S

L

L

K

L

D

D

S

S

I

I

Q

R

F

I

I

I

T

P

T

V

R

R

H

H

E
|
E

Q

A

G

N

A

A

F

F

M

M

A

A

D

A

V

A

Y

V

G

G

R

R

L

I

T

T

G

G

K

K

A

A

G

G

V

V

C

A

L

L

S

V

T
|
T

L

S

G

G

P

P

G

G

A

C

T

S

N

N

L

L

M

I

T

T

G

G

V

M

A

A

D

T

A

A

N

N

L

S

D

E

G

G

A

D

P

P

L

V

V

V

A

A

I

L

T

G

G

G

Q

A

V

V

G

K

T

R

D

A

R

D

M

K

H

A

I

K

E

Q

S

V

H
|
H

Q
|
Q

Y
x
S

L

M

D

D

L

T

V

V

A

A

M

M

F

F

A

S

P

P

V

V

T

T

K

K

W

Y

N

A

K

I

Q

E

I

V

V

T

R

A

P

P

S

D

I

A

T

L

P

A

E

E

V

V

T

V

R

S

K

N

A

A

F

F

K

R

L

A

A

A

Q

E

S

Q

E

G

K

R

P

P

G

G

A

S

V

A

H

F

I

V

D

S

L

L

P

P

E
x
Q

N

D

I

V

A

V

A

D

L

G

P

P

V

V

E

S

G

G

W

K

P

V

L

L

T

P

R

-

D

-

S

A

Q

P

E

Q

K

M

T

G

Y

A

A

A

S

P

Y

D

R

D

S

A

L

I

N

D

A

Q

A

V

A

A

M

K

A

L

I

I

S

A

K

Q

A

A

K

K

N

N

P

P

L

I

I

F

L

L

V

L

G

G

N

L

G

M

T

A

I

S

R

Q

A

P

H

E

A

N

S

S

E

K

A

A

L

L

T

R

E

R

F

L

A

L

T

E

T

T

L

S

N

H

I

I

P

P

V

V

A

T

N

S

T

T

F

Y

M

Q

G

A

K

A

G

G

A

A

V

V

P

N

F

Q

T

D

H

N

P

-

L

F

S

S

L

R

W

F

T

A

-
x
G

-
x
R

V

V

G

G

L
|
L

Q

F

Q

N

R

N

D
x
Q

L

A

I

G

T

D

C

R

A

L

F

L

E

Q

Q

L

S

A

D

D

L

L

V

V

I

I

A

C

V

I

G

G

Y

Y

D

S

L

P

I

V

E

E

Y

Y

S
x
E

P

P

K

A

K

M

W

W

N

N

P

-

E

S

G

G

T

N

T

A

P

T

I

L

I

V

H

H

I

I

G

D

M

V

T

L

P

P

A

A

E

Y

I

E

D

E

S

R

S

N

Y

Y

I

T

P

P

L

D

V

V

E

E

V

L

V

V

G

G

D

D

I

I

S

A

D

G

S

T

L

L

M

N

D

K

I

L

L

A

K

Q

R

N

A

I

D

D

R

H

Q

R

G

L

K

V

S

L

T

S

S

P

V

Q

A

A

G

E

L

I

R

L

S

R

D

D

L

-

V

-

R
|
R

E

Q

Y

H

E

Q

K

R

Y

E

A

L

H

L

D

D

E

R

G

R

F

G

P

A

V

Q

K

L

P

N

Q

Q

K

F

I

A

I

L

Y

H

D

P

L

L

R

R

Q

I

V

V

M

R

G

A

P

M

E

Q

D

D

I

I

V

V

I

N

S

S

D

D

V

V

-

T

-

L

-

T

-

V

-

D

-
x
M

G
|
G

A
x
S

H
x
F

K

H

M

I

W

W

M

I

A

A

R
|
R

H

Y

Y
x
L

H
x
Y

C

T

D

F

T

R

P

A

N

R

T

Q

C

V

L

M

I

I

S

S

N

N

G

G

F

Q

A
x
Q

A

T

M
|
M

G

G

I

V

A

A

I

L

P

P

G

W

A

A

I

I

A

G

A

A

K

W

L

L

V

V

A

N

P

P

D

E

K

R

K

K

V

V

A

S

V

V

T

S

G
|
G

D
|
D

G
|
G

G

G

F

F

M

L

M

Q

N

S

C

S

Q

M

E

E

L

L

E

E

T

T

A

A

L

V

R

R

A

L

G

K

T

A

P

N

F

V

V

L

T

H

L

L

I

I

F

W

N

V

D
|
D

N

N

G
|
G

Y
|
Y

G
x
N

L
x
M

I
x
V

E

A

W

I

K
x
Q

Q

E

I

E

N

K

Q

K

F

Y

G

Q

Q

R

S

L

S

S

F

G

I

V

K

E

F

F

T

G

N

P

P

M

D

D

F

F

V

K

K

A

F

Y

A

A

E

E

S

S

M

F

G

G

L

A

K

K

G

G

Y

F

R

A

V

V

E

E

S

S

A

A

D

E

D

A

L

L

V

E

P

P

T

T

L

L

K

R

T

A

A

A

L

M

E

D

Q

V

G

D

V

G

P

P

A

A

V

V

I

V

D

A

C

I

P

P

V

V

D

D

Y
|
Y

Q

R

E

D

N

N

active site: I26 (≠ L25), G28 (= G27), A29 (≠ E28), K30 (≠ E29), I31 (≠ N30), E51 (= E50), T74 (= T73), H113 (= H112), Q114 (= Q113), S115 (≠ Y114), Q163 (≠ E162), L253 (= L253), E280 (≠ S280), M385 (vs. gap), Q411 (≠ A408), M413 (= M410), D438 (= D435), D465 (= D462), G467 (= G464), Y468 (= Y465), M470 (≠ L467), V471 (≠ I468), Q474 (≠ K471), Y534 (= Y531)
binding magnesium ion: D438 (= D435), D465 (= D462), G467 (= G464)
binding phosphate ion: G249 (vs. gap), R250 (vs. gap), Q257 (≠ D257), R343 (= R346), R394 (= R391), L396 (≠ Y393), Y397 (≠ H394)
binding thiamine diphosphate: G386 (= G383), S387 (≠ A384), F388 (≠ H385), Q411 (≠ A408), M413 (= M410), G437 (= G434), D438 (= D435), G439 (= G436), D465 (= D462), G467 (= G464), Y468 (= Y465), N469 (≠ G466), M470 (≠ L467), V471 (≠ I468), Y534 (= Y531)

1ozgA The crystal structure of klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactor and with an unusual intermediate (see paper)
39% identity, 96% coverage: 7:534/550 of query aligns to 9:541/549 of 1ozgA

query
sites
1ozgA

A

A

E

D

L

L

L

V

V

V

K

S

C

Q

L

L

E

E

N

A

E

Q

G

G

V

V

E

R

Y

Q

I

V

F

F

G

G

L
x
I

P

P

G
|
G

E
x
A

E
x
K

N
x
I

L

D

H

K

I

V

L

F

E

D

A

S

L

L

K

L

D

D

S

S

I

I

Q

R

F

I

I

I

T

P

T

V

R

R

H

H

E
|
E

Q

A

G

N

A

A

F

F

M

M

A

A

D

A

V

A

Y

V

G

G

R

R

L

I

T

T

G

G

K

K

A

A

G

G

V

V

C

A

L

L

S

V

T
|
T

L

S

G

G

P

P

G

G

A

C

T

S

N

N

L

L

M

I

T

T

G

G

V

M

A

A

D

T

A

A

N

N

L

S

D

E

G

G

A

D

P

P

L

V

V

V

A

A

I

L

T

G

G

G

Q

A

V

V

G

K

T

R

D

A

R

D

M

K

H

A

I

K

E

Q

S

V

H
|
H

Q
|
Q

Y
x
S

L

M

D

D

L

T

V

V

A

A

M

M

F

F

A

S

P

P

V

V

T

T

K

K

W

Y

N

A

K

I

Q

E

I

V

V

T

R

A

P

P

S

D

I

A

T

L

P

A

E

E

V

V

T

V

R

S

K

N

A

A

F

F

K

R

L

A

A

A

Q

E

S

Q

E

G

K

R

P

P

G

G

A

S

V

A

H

F

I

V

D

S

L

L

P

P

E
x
Q

N

D

I

V

A

V

A

D

L

G

P

P

V

V

E

S

G

G

W

K

P

V

L

L

-

P

T

A

R

S

D

G

S

A

Q

P

E

Q

K

M

T

G

Y

A

A

A

S

P

Y

D

R

D

S

A

L

I

N

D

A

Q

A

V

A

A

M

K

A

L

I

I

S

A

K

Q

A

A

K

K

N

N

P

P

L

I

I

F

L

L

V

L

G

G

N

L

G

M

T

A

I

S

R

Q

A

P

H

E

A

N

S

S

E

K

A

A

L

L

T

R

E

R

F

L

A

L

T

E

T

T

L

S

N

H

I

I

P

P

V

V

A

T

N

S

T

T

F

Y

M

Q

G

A

K

A

G

G

A

A

V

V

P

N

F

Q

T

D

H

N

P

-

L

F

S

S

L

R

W

F

T

A

-
x
G

-
x
R

V

V

G

G

L
|
L

Q

F

Q

N

R

N

D
x
Q

L

A

I

G

T

D

C

R

A

L

F

L

E

Q

Q

L

S

A

D

D

L

L

V

V

I

I

A

C

V

I

G

G

Y

Y

D

S

L

P

I

V

E

E

Y

Y

S
x
E

P

P

K

A

K

M

W

W

N

N

P

-

E

S

G

G

T

N

T

A

P

T

I

L

I

V

H

H

I

I

G

D

M

V

T

L

P

P

A

A

E

Y

I

E

D

E

S

R

S

N

Y

Y

I

T

P

P

L

D

V

V

E

E

V

L

V

V

G

G

D

D

I

I

S

A

D

G

S

T

L

L

M

N

D

K

I

L

L

A

K

Q

R

N

A

I

D

D

R

H

Q

R

G

L

K

V

S

L

T

S

S

P

V

Q

A

A

G

E

L

I

R

L

S

R

D

D

L

-

V

-

R
|
R

E

Q

Y

H

E

Q

K

R

Y

E

A

L

H

L

D

D

E

R

G

R

F

G

P

A

V

Q

K

L

P

N

Q

Q

K

F

I

A

I

L

Y

H

D

P

L

L

R

R

Q

I

V

V

M

R

G

A

P

M

E

Q

D

D

I

I

V

V

I

N

S

S

D

D

V

V

-

T

-

L

-

T

-

V

-

D

-
x
M

G
|
G

A
x
S

H
x
F

K

H

M

I

W

W

M

I

A

A

R
|
R

H

Y

Y

L

H
x
Y

C

T

D

F

T

R

P

A

N

R

T

Q

C

V

L

M

I

I

S

S

N

N

G

G

F

Q

A
x
Q

A

T

M
|
M

G

G

I

V

A

A

I

L

P

P

G

W

A

A

I

I

A

G

A

A

K

W

L

L

V

V

A

N

P

P

D

E

K

R

K

K

V

V

A

S

V

V

T

S

G
|
G

D
|
D

G
|
G

G

G

F

F

M

L

M

Q

N

S

C

S

Q

M

E

E

L

L

E

E

T

T

A

A

L

V

R

R

A

L

G

K

T

A

P

N

F

V

V

L

T

H

L

L

I

I

F

W

N

V

D
|
D

N

N

G
|
G

Y
|
Y

G
x
N

L
x
M

I
x
V

E

A

W

I

K
x
Q

Q

E

I

E

N

K

Q

K

F

Y

G

Q

Q

R

S

L

S

S

F

G

I

V

K

E

F

F

T

G

N

P

P

M

D

D

F

F

V

K

K

A

F

Y

A

A

E

E

S

S

M

F

G

G

L

A

K

K

G

G

Y

F

R

A

V

V

E

E

S

S

A

A

D

E

D

A

L

L

V

E

P

P

T

T

L

L

K

R

T

A

A

A

L

M

E

D

Q

V

G

D

V

G

P

P

A

A

V

V

I

V

D

A

C

I

P

P

V

V

D

D

Y
|
Y

Q

R

E

D

N

N

active site: I27 (≠ L25), G29 (= G27), A30 (≠ E28), K31 (≠ E29), I32 (≠ N30), E52 (= E50), T75 (= T73), H114 (= H112), Q115 (= Q113), S116 (≠ Y114), Q164 (≠ E162), L257 (= L253), E284 (≠ S280), M389 (vs. gap), Q415 (≠ A408), M417 (= M410), D442 (= D435), D469 (= D462), G471 (= G464), Y472 (= Y465), M474 (≠ L467), V475 (≠ I468), Q478 (≠ K471), Y538 (= Y531)
binding 2-hydroxyethyl dihydrothiachrome diphosphate: M389 (vs. gap), G390 (= G383), S391 (≠ A384), F392 (≠ H385), Q415 (≠ A408), M417 (= M410), G441 (= G434), D442 (= D435), G443 (= G436), D469 (= D462), G471 (= G464), Y472 (= Y465), N473 (≠ G466), M474 (≠ L467), V475 (≠ I468), Y538 (= Y531)
binding magnesium ion: D442 (= D435), D469 (= D462), G471 (= G464)
binding phosphate ion: G253 (vs. gap), R254 (vs. gap), Q261 (≠ D257), R347 (= R346), R398 (= R391), Y401 (≠ H394)

5wdgA Acetolactate synthase from klebsiella pneumoniae in complex with a reaction intermediate
39% identity, 96% coverage: 7:534/550 of query aligns to 9:530/538 of 5wdgA

query
sites
5wdgA

A

A

E

D

L

L

L

V

V

V

K

S

C

Q

L

L

E

E

N

A

E

Q

G

G

V

V

E

R

Y

Q

I

V

F

F

G

G

L
x
I

P

P

G
|
G

E
x
A

E
x
K

N
x
I

L

D

H

K

I

V

L

F

E

D

A

S

L

L

K

L

D

D

S

S

I

I

Q

R

F

I

I

I

T

P

T

V

R

R

H

H

E
|
E

Q

A

G

N

A

A

F

F

M

M

A

A

D

A

V

A

Y

V

G

G

R
|
R

L

I

T

T

G

G

K

K

A

A

G

G

V

V

C

A

L

L

S

V

T
|
T

L

S

G

G

P

P

G

G

A

C

T

S

N

N

L

L

M

I

T

T

G

G

V

M

A

A

D

T

A

A

N

N

L

S

D

E

G
|
G

A

D

P

P

L

V

V

V

A

A

I

L

T

G

G

G

Q

A

V

V

G

K

-

R

T

A

D

D

R

K

M

M

H

-

I

-

E

-

S

-

H

-

Q

-

Y

-

L

-

D

D

L

T

V

V

A

A

M

M

F

F

A
x
S

P

P

V

V

T
|
T

K

K

W

Y

N

A

K

I

Q

E

I

V

V

T

R

A

P

P

S

D

I

A

T

L

P

A

E

E

V

V

T

V

R

S

K

N

A

A

F

F

K

R

L

A

A

A

Q

E

S

Q

E

G

K
x
R

P

P

G

G

A

S

V

A

H

F

I

V

D

S

L

L

P

P

E
x
Q

N

D

I

V

A

V

A

D

L

G

P

P

V

V

E

S

G

G

W

K

P

V

L

L

T

-

R

-

D

-

S

P

Q

A

E

Q

K

M

T

G

Y

A

A

A

S

P

Y

D

R

D

S

A

L

I

N

D

A

Q

A

V

A

A

M

K

A

L

I

I

S

A

K

Q

A

A

K

K

N

N

P

P

L

I

I

F

L

L

V

L

G

G

N

L

G

M

T

A

I

S

R
x
Q

A

P

H

E

A

N

S

S

E

K

A

A

L

L

T

R

E

R

F

L

A

L

T

E

T

T

L

S

N

H

I

I

P

P

V

V

A

T

N

S

T

T

F

Y

M

Q

G

A

K

A

G

G

A

A

V

V

P

N

F

Q

T

D

H

N

P

-

L

F

S

S

L

R

W

F

T

A

-

G

-

R

V

V

G

G

L
|
L

Q

F

Q

N

R

N

D

Q

L

A

I

G

T

D

C

R

A

L

F

L

E

Q

Q

L

S

A

D

D

L

L

V

V

I

I

A

C

V

I

G

G

Y

Y

D

S

L

P

I

V

E

E

Y

Y

S
x
E

P

P

K

A

K

M

W

W

N

N

P

-

E

S

G

G

T

N

T

A

P

T

I

L

I

V

H

H

I

I

G

D

M

V

T

L

P

P

A

A

E

Y

I

E

D

E

S

R

S

N

Y

Y

I

T

P

P

L

D

V

V

E

E

V

L

V

V

G

G

D

D

I

I

S

A

D

G

S

T

L

L

M

N

D

K

I

L

L

A

K

Q

R

N

A

I

D

D

R

H

Q

R

G

L

K

V

S

L

T

S

S

P

V

Q

A

A

G

E

L

I

R

L

S

R

D

D

L

-

V

-

R

R

E

Q

Y

H

E

Q

K

R

Y

E

A

L

H

L

D

D

E

R

G

R

F

G

P

A

V

Q

K

L

P

N

Q

Q

K

F

I

A

I

L

Y

H

D

P

L

L

R

R

Q

I

V

V

M

R

G

A

P

M

E

Q

D

D

I

I

V

V

I

N

S

S

D

D

V

V

-

T

-

L

-

T

-

V

-

D

-
x
M

G

G

A
x
S

H
x
F

K

H

M

I

W

W

M

I

A

A

R

R

H

Y

Y

L

H

Y

C

S

D

F

T

R

P

A

N

R

T

Q

C

V

L

M

I

I

S

S

N

N

G

G

F

Q

A
x
Q

A

T

M
|
M

G

G

I

V

A

A

I

L

P

P

G

W

A

A

I

I

A

G

A

A

K

W

L

L

V

V

A

N

P

P

D

E

K

R

K

K

V

V

A

S

V

V

T

S

G
|
G

D
|
D

G
|
G

F

F

M

L

M

Q

N

S

C

S

Q

M

E

E

L

L

E

E

T

T

A

A

L

V

R

R

A

L

G

K

T

A