SitesBLAST
Comparing WP_009543700.1 NCBI__GCF_000017845.1:WP_009543700.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4rjkG Acetolactate synthase from bacillus subtilis bound to lthdp - crystal form ii (see paper)
36% identity, 97% coverage: 7:538/550 of query aligns to 4:540/553 of 4rjkG
- binding magnesium ion: D437 (= D435), D464 (= D462), T466 (≠ G464)
- binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-carboxy-1-hydroxyethyl)-5-(2-{[hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: E47 (= E50), Q110 (= Q113)
- binding thiamine diphosphate: I384 (≠ V382), G385 (= G383), S386 (≠ A384), H387 (= H385), Q410 (≠ A408), L412 (≠ M410), G436 (= G434), D437 (= D435), G438 (= G436), G439 (= G437), T466 (≠ G464), Y467 (= Y465), D468 (≠ G466), M469 (≠ L467), V470 (≠ I468), Y533 (= Y531)
4rjkF Acetolactate synthase from bacillus subtilis bound to lthdp - crystal form ii (see paper)
36% identity, 97% coverage: 7:538/550 of query aligns to 4:540/552 of 4rjkF
- binding magnesium ion: D437 (= D435), D464 (= D462), T466 (≠ G464)
- binding pyruvic acid: A25 (≠ E28), K26 (≠ E29)
- binding thiamine diphosphate: P23 (= P26), E47 (= E50), P73 (= P76), G385 (= G383), S386 (≠ A384), H387 (= H385), Q410 (≠ A408), L412 (≠ M410), G436 (= G434), D437 (= D435), G438 (= G436), G439 (= G437), T466 (≠ G464), Y467 (= Y465), D468 (≠ G466), M469 (≠ L467), V470 (≠ I468), Y533 (= Y531)
4rjiC Acetolactate synthase from bacillus subtilis bound to thdp - crystal form i (see paper)
36% identity, 97% coverage: 7:538/550 of query aligns to 5:541/555 of 4rjiC
- binding magnesium ion: D438 (= D435), D465 (= D462), T467 (≠ G464)
- binding thiamine diphosphate: P24 (= P26), E48 (= E50), P74 (= P76), S387 (≠ A384), H388 (= H385), Q411 (≠ A408), G437 (= G434), D438 (= D435), G439 (= G436), G440 (= G437), T467 (≠ G464), Y468 (= Y465), D469 (≠ G466), M470 (≠ L467), V471 (≠ I468), Y534 (= Y531)
5d6rB Acetolactate synthase from klebsiella pneumoniae in complex with mechanism-based inhibitor
39% identity, 96% coverage: 7:534/550 of query aligns to 8:538/548 of 5d6rB
- active site: I26 (≠ L25), G28 (= G27), A29 (≠ E28), K30 (≠ E29), I31 (≠ N30), E51 (= E50), T74 (= T73), H113 (= H112), Q114 (= Q113), S115 (≠ Y114), Q163 (≠ E162), L254 (= L253), E281 (≠ S280), M386 (vs. gap), Q412 (≠ A408), M414 (= M410), D439 (= D435), D466 (= D462), G468 (= G464), Y469 (= Y465), M471 (≠ L467), V472 (≠ I468), Q475 (≠ K471), Y535 (= Y531)
- binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-[(Z)-2-fluoro-1-hydroxy-2-phosphonoethenyl]-5-(2-{[(S)-hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: M386 (vs. gap), G387 (= G383), S388 (≠ A384), Q412 (≠ A408), M414 (= M410), D439 (= D435), G440 (= G436), G468 (= G464), Y469 (= Y465), N470 (≠ G466), M471 (≠ L467), Y535 (= Y531)
- binding magnesium ion: R63 (= R62), Q212 (≠ R212), D439 (= D435), D466 (= D462), G468 (= G464)
5dx6B Acetolactate synthase from klebsiella pneumoniae soaked with beta- fluoropyruvate
38% identity, 96% coverage: 7:534/550 of query aligns to 20:544/557 of 5dx6B
- active site: I38 (≠ L25), G40 (= G27), A41 (≠ E28), K42 (≠ E29), I43 (≠ N30), E63 (= E50), T86 (= T73), H125 (= H112), Q126 (= Q113), S127 (≠ Y114), Q175 (≠ E162), L268 (= L253), E295 (≠ S280), M392 (≠ V382), Q418 (≠ A408), M420 (= M410), D445 (= D435), D472 (= D462), G474 (= G464), Y475 (= Y465), M477 (≠ L467), V478 (≠ I468), Q481 (≠ K471), Y541 (= Y531)
- binding 3-fluoro-2-oxopropanoic acid: G264 (vs. gap), R265 (vs. gap), Q272 (≠ D257), A400 (= A390), R401 (= R391), Y404 (≠ H394)
- binding magnesium ion: S135 (≠ A122), T138 (= T125), D445 (= D435), D472 (= D462), G474 (= G464)
- binding thiamine diphosphate: G393 (= G383), S394 (≠ A384), F395 (≠ H385), Q418 (≠ A408), M420 (= M410), G444 (= G434), D445 (= D435), G446 (= G436), D472 (= D462), G474 (= G464), Y475 (= Y465), N476 (≠ G466), M477 (≠ L467), V478 (≠ I468), Y541 (= Y531)
1ozfA The crystal structure of klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactors (see paper)
39% identity, 96% coverage: 7:534/550 of query aligns to 8:537/545 of 1ozfA
- active site: I26 (≠ L25), G28 (= G27), A29 (≠ E28), K30 (≠ E29), I31 (≠ N30), E51 (= E50), T74 (= T73), H113 (= H112), Q114 (= Q113), S115 (≠ Y114), Q163 (≠ E162), L253 (= L253), E280 (≠ S280), M385 (vs. gap), Q411 (≠ A408), M413 (= M410), D438 (= D435), D465 (= D462), G467 (= G464), Y468 (= Y465), M470 (≠ L467), V471 (≠ I468), Q474 (≠ K471), Y534 (= Y531)
- binding magnesium ion: D438 (= D435), D465 (= D462), G467 (= G464)
- binding phosphate ion: G249 (vs. gap), R250 (vs. gap), Q257 (≠ D257), R343 (= R346), R394 (= R391), L396 (≠ Y393), Y397 (≠ H394)
- binding thiamine diphosphate: G386 (= G383), S387 (≠ A384), F388 (≠ H385), Q411 (≠ A408), M413 (= M410), G437 (= G434), D438 (= D435), G439 (= G436), D465 (= D462), G467 (= G464), Y468 (= Y465), N469 (≠ G466), M470 (≠ L467), V471 (≠ I468), Y534 (= Y531)
1ozgA The crystal structure of klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactor and with an unusual intermediate (see paper)
39% identity, 96% coverage: 7:534/550 of query aligns to 9:541/549 of 1ozgA
- active site: I27 (≠ L25), G29 (= G27), A30 (≠ E28), K31 (≠ E29), I32 (≠ N30), E52 (= E50), T75 (= T73), H114 (= H112), Q115 (= Q113), S116 (≠ Y114), Q164 (≠ E162), L257 (= L253), E284 (≠ S280), M389 (vs. gap), Q415 (≠ A408), M417 (= M410), D442 (= D435), D469 (= D462), G471 (= G464), Y472 (= Y465), M474 (≠ L467), V475 (≠ I468), Q478 (≠ K471), Y538 (= Y531)
- binding 2-hydroxyethyl dihydrothiachrome diphosphate: M389 (vs. gap), G390 (= G383), S391 (≠ A384), F392 (≠ H385), Q415 (≠ A408), M417 (= M410), G441 (= G434), D442 (= D435), G443 (= G436), D469 (= D462), G471 (= G464), Y472 (= Y465), N473 (≠ G466), M474 (≠ L467), V475 (≠ I468), Y538 (= Y531)
- binding magnesium ion: D442 (= D435), D469 (= D462), G471 (= G464)
- binding phosphate ion: G253 (vs. gap), R254 (vs. gap), Q261 (≠ D257), R347 (= R346), R398 (= R391), Y401 (≠ H394)
5wdgA Acetolactate synthase from klebsiella pneumoniae in complex with a reaction intermediate
39% identity, 96% coverage: 7:534/550 of query aligns to 9:530/538 of 5wdgA
- active site: I27 (≠ L25), G29 (= G27), A30 (≠ E28), K31 (≠ E29), I32 (≠ N30), E52 (= E50), T75 (= T73), Q157 (≠ E162), L246 (= L253), E273 (≠ S280), M378 (vs. gap), Q404 (≠ A408), M406 (= M410), D431 (= D435), D458 (= D462), G460 (= G464), Y461 (= Y465), M463 (≠ L467), V464 (≠ I468), Q467 (≠ K471), Y527 (= Y531)
- binding (2S,3S)-2,3-dihydroxy-3-[(7S,8R,9aS)-8-(2-{[(R)-hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-2,7-dimethyl-5,7,8,10-tetrahydro-9aH-pyrimido[4,5-d][1,3]thiazolo[3,2-a]pyrimidin-9a-yl]-2-methylbutanoic acid: M378 (vs. gap), S380 (≠ A384), F381 (≠ H385), Q404 (≠ A408), M406 (= M410), G430 (= G434), D431 (= D435), G432 (= G436), G433 (= G437), D458 (= D462), G460 (= G464), Y461 (= Y465), N462 (≠ G466), M463 (≠ L467), V464 (≠ I468), Y527 (= Y531)
- binding magnesium ion: R64 (= R62), S117 (≠ A122), T120 (= T125), Q204 (≠ R212), D431 (= D435), D458 (= D462), G460 (= G464)
- binding pyruvic acid: G94 (= G92), R147 (≠ K152)
5dx6A Acetolactate synthase from klebsiella pneumoniae soaked with beta- fluoropyruvate
38% identity, 96% coverage: 7:534/550 of query aligns to 9:533/541 of 5dx6A
- active site: I27 (≠ L25), G29 (= G27), A30 (≠ E28), K31 (≠ E29), I32 (≠ N30), E52 (= E50), T75 (= T73), Q159 (≠ E162), L249 (= L253), E276 (≠ S280), M381 (vs. gap), Q407 (≠ A408), M409 (= M410), D434 (= D435), D461 (= D462), G463 (= G464), Y464 (= Y465), M466 (≠ L467), V467 (≠ I468), Q470 (≠ K471), Y530 (= Y531)
- binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-[(1R)-2-fluoro-1-hydroxyethyl]-5-(2-{[(S)-hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: M381 (vs. gap), G382 (= G383), S383 (≠ A384), F384 (≠ H385), Q407 (≠ A408), M409 (= M410), G433 (= G434), D434 (= D435), G435 (= G436), D461 (= D462), G463 (= G464), Y464 (= Y465), N465 (≠ G466), Y530 (= Y531)
- binding magnesium ion: S119 (≠ A122), T122 (= T125), D434 (= D435), D461 (= D462), G463 (= G464)
P09114 Acetolactate synthase 2, chloroplastic; ALS II; Acetohydroxy-acid synthase II; Acetolactate synthase II; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see paper)
34% identity, 96% coverage: 7:535/550 of query aligns to 94:641/664 of P09114
- P191 (≠ G103) mutation to A: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with L-568.
- W568 (= W470) mutation to L: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with A-191.
7tzzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase p197t mutant in complex with bispyribac-sodium (see paper)
33% identity, 96% coverage: 7:535/550 of query aligns to 15:562/582 of 7tzzA
- binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: M266 (≠ L253), R292 (≠ Y279), W489 (= W470)
- binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V382), G401 (= G383), Q402 (≠ A384), H403 (= H385), G426 (≠ A408), M428 (= M410), G452 (= G434), D453 (= D435), G454 (= G436), S455 (≠ G437), L483 (≠ Y465), G484 (= G466), M485 (≠ L467), V486 (≠ I468)
- binding flavin-adenine dinucleotide: R161 (≠ K152), G222 (= G207), G223 (≠ N208), G224 (= G209), T246 (= T233), L247 (≠ F234), M248 (= M235), M263 (≠ T250), L264 (≠ V251), M266 (≠ L253), H267 (≠ Q254), G286 (= G273), R288 (≠ D275), V293 (≠ S280), D310 (≠ G296), I311 (≠ M297), D329 (= D315), V330 (≠ I316), M405 (= M387), G423 (≠ N405)
- binding magnesium ion: A37 (≠ E29), T82 (= T73), S83 (≠ L74), Q122 (= Q113), Y381 (≠ K363), D453 (= D435), M458 (= M440), Q461 (= Q443), N480 (≠ D462), H482 (≠ G464), K533 (≠ A506)
Sites not aligning to the query:
5k3sA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, bispyribac-sodium (see paper)
33% identity, 96% coverage: 7:535/550 of query aligns to 15:562/583 of 5k3sA
- active site: Y33 (≠ L25), G35 (= G27), G36 (≠ E28), A37 (≠ E29), S38 (≠ N30), E59 (= E50), T82 (= T73), F121 (≠ H112), Q122 (= Q113), E123 (≠ Y114), K171 (≠ E162), M266 (≠ L253), V293 (≠ S280), V400 (= V382), G426 (≠ A408), M428 (= M410), D453 (= D435), N480 (≠ D462), H482 (≠ G464), L483 (≠ Y465), M485 (≠ L467), V486 (≠ I468), W489 (= W470), H558 (≠ Y531)
- binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: R292 (≠ Y279), M485 (≠ L467), W489 (= W470)
- binding flavin-adenine dinucleotide: R161 (≠ K152), G222 (= G207), G223 (≠ N208), G224 (= G209), T246 (= T233), L247 (≠ F234), M248 (= M235), L264 (≠ V251), M266 (≠ L253), G286 (= G273), R288 (≠ D275), D290 (≠ I277), V293 (≠ S280), D310 (≠ G296), I311 (≠ M297), D329 (= D315), V330 (≠ I316), M405 (= M387), G423 (≠ N405)
- binding magnesium ion: D453 (= D435), N480 (≠ D462), H482 (≠ G464)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V382), G401 (= G383), Q402 (≠ A384), H403 (= H385), G426 (≠ A408), M428 (= M410), D453 (= D435), G454 (= G436), S455 (≠ G437), N480 (≠ D462), H482 (≠ G464), L483 (≠ Y465), G484 (= G466), M485 (≠ L467), V486 (≠ I468)
Sites not aligning to the query:
8et4A Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with amidosulfuron (see paper)
33% identity, 96% coverage: 7:535/550 of query aligns to 15:562/582 of 8et4A
- binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V382), G401 (= G383), Q402 (≠ A384), H403 (= H385), G426 (≠ A408), M428 (= M410), G452 (= G434), D453 (= D435), G454 (= G436), S455 (≠ G437), M458 (= M440), N480 (≠ D462), H482 (≠ G464), L483 (≠ Y465), G484 (= G466), M485 (≠ L467), V486 (≠ I468)
- binding flavin-adenine dinucleotide: R161 (≠ K152), G222 (= G207), G223 (≠ N208), G224 (= G209), T246 (= T233), L247 (≠ F234), M248 (= M235), L264 (≠ V251), M266 (≠ L253), H267 (≠ Q254), G286 (= G273), V287 (≠ Y274), R288 (≠ D275), D290 (≠ I277), R292 (≠ Y279), V293 (≠ S280), D310 (≠ G296), I311 (≠ M297), D329 (= D315), V330 (≠ I316), M405 (= M387), G423 (≠ N405)
- binding magnesium ion: F370 (vs. gap), D453 (= D435), M458 (= M440), Q461 (= Q443), N480 (≠ D462), H482 (≠ G464), K533 (≠ A506)
- binding N-{[(4,6-dimethoxypyrimidin-2-yl)carbamoyl]sulfamoyl}-N-methylmethanesulfonamide: M266 (≠ L253), R292 (≠ Y279), M485 (≠ L467), W489 (= W470)
Sites not aligning to the query:
5wj1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a triazolopyrimidine herbicide, penoxsulam (see paper)
33% identity, 96% coverage: 7:535/550 of query aligns to 15:562/582 of 5wj1A
- active site: Y33 (≠ L25), G35 (= G27), G36 (≠ E28), A37 (≠ E29), S38 (≠ N30), E59 (= E50), T82 (= T73), F121 (≠ H112), Q122 (= Q113), E123 (≠ Y114), K171 (≠ E162), M266 (≠ L253), V293 (≠ S280), V400 (= V382), G426 (≠ A408), M428 (= M410), D453 (= D435), N480 (≠ D462), H482 (≠ G464), L483 (≠ Y465), M485 (≠ L467), V486 (≠ I468), W489 (= W470), H558 (≠ Y531)
- binding flavin-adenine dinucleotide: R161 (≠ K152), G222 (= G207), G223 (≠ N208), G224 (= G209), T246 (= T233), L247 (≠ F234), M248 (= M235), M263 (≠ T250), L264 (≠ V251), G286 (= G273), R288 (≠ D275), V293 (≠ S280), D310 (≠ G296), I311 (≠ M297), D329 (= D315), V330 (≠ I316), M405 (= M387), G423 (≠ N405), G424 (= G406)
- binding magnesium ion: D453 (= D435), N480 (≠ D462), H482 (≠ G464)
- binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: M266 (≠ L253), D291 (≠ E278), R292 (≠ Y279), M485 (≠ L467), W489 (= W470)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V382), G401 (= G383), Q402 (≠ A384), H403 (= H385), M428 (= M410), D453 (= D435), G454 (= G436), S455 (≠ G437), M458 (= M440), N480 (≠ D462), H482 (≠ G464), L483 (≠ Y465), G484 (= G466), M485 (≠ L467), V486 (≠ I468)
Sites not aligning to the query:
5k6tA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, propoxycarbazone-sodium (see paper)
33% identity, 96% coverage: 7:535/550 of query aligns to 15:562/582 of 5k6tA
- active site: Y33 (≠ L25), G35 (= G27), G36 (≠ E28), A37 (≠ E29), S38 (≠ N30), E59 (= E50), T82 (= T73), F121 (≠ H112), Q122 (= Q113), E123 (≠ Y114), K171 (≠ E162), M266 (≠ L253), V293 (≠ S280), V400 (= V382), G426 (≠ A408), M428 (= M410), D453 (= D435), N480 (≠ D462), H482 (≠ G464), L483 (≠ Y465), M485 (≠ L467), V486 (≠ I468), W489 (= W470), H558 (≠ Y531)
- binding methyl 2-[(4-methyl-5-oxidanylidene-3-propoxy-1,2,4-triazol-1-yl)carbonylsulfamoyl]benzoate: H267 (≠ Q254), R292 (≠ Y279), M485 (≠ L467), W489 (= W470)
- binding flavin-adenine dinucleotide: R161 (≠ K152), G222 (= G207), G223 (≠ N208), G224 (= G209), T246 (= T233), L247 (≠ F234), M248 (= M235), L264 (≠ V251), G286 (= G273), R288 (≠ D275), D290 (≠ I277), R292 (≠ Y279), V293 (≠ S280), D310 (≠ G296), I311 (≠ M297), D329 (= D315), V330 (≠ I316), Q404 (≠ K386), M405 (= M387), G423 (≠ N405)
- binding magnesium ion: D453 (= D435), N480 (≠ D462), H482 (≠ G464)
- binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (= V382), G401 (= G383), Q402 (≠ A384), H403 (= H385), G426 (≠ A408), M428 (= M410), G452 (= G434), G454 (= G436), S455 (≠ G437), N480 (≠ D462), H482 (≠ G464), L483 (≠ Y465), G484 (= G466)
Sites not aligning to the query:
5k6rA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, thiencarbazone-methyl (see paper)
33% identity, 96% coverage: 7:535/550 of query aligns to 15:562/582 of 5k6rA
- active site: Y33 (≠ L25), G35 (= G27), G36 (≠ E28), A37 (≠ E29), S38 (≠ N30), E59 (= E50), T82 (= T73), F121 (≠ H112), Q122 (= Q113), E123 (≠ Y114), K171 (≠ E162), M266 (≠ L253), V293 (≠ S280), V400 (= V382), G426 (≠ A408), M428 (= M410), D453 (= D435), N480 (≠ D462), H482 (≠ G464), L483 (≠ Y465), M485 (≠ L467), V486 (≠ I468), W489 (= W470), H558 (≠ Y531)
- binding methyl 4-[(3-methoxy-4-methyl-5-oxidanylidene-1,2,4-triazol-1-yl)carbonylsulfamoyl]-5-methyl-thiophene-3-carboxylate: R292 (≠ Y279), W489 (= W470)
- binding flavin-adenine dinucleotide: R161 (≠ K152), G222 (= G207), G223 (≠ N208), G224 (= G209), T246 (= T233), L247 (≠ F234), M248 (= M235), L264 (≠ V251), M266 (≠ L253), G286 (= G273), R288 (≠ D275), R292 (≠ Y279), V293 (≠ S280), D310 (≠ G296), I311 (≠ M297), G328 (= G314), D329 (= D315), V330 (≠ I316), M405 (= M387), G423 (≠ N405)
- binding magnesium ion: D453 (= D435), N480 (≠ D462), H482 (≠ G464)
- binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (= V382), G401 (= G383), Q402 (≠ A384), H403 (= H385), G426 (≠ A408), M428 (= M410), D453 (= D435), G454 (= G436), S455 (≠ G437), M458 (= M440), N480 (≠ D462), H482 (≠ G464), L483 (≠ Y465), G484 (= G466), M485 (≠ L467), V486 (≠ I468)
Sites not aligning to the query:
1z8nA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with an imidazolinone herbicide, imazaquin (see paper)
33% identity, 96% coverage: 7:535/550 of query aligns to 15:562/582 of 1z8nA
- active site: Y33 (≠ L25), G35 (= G27), G36 (≠ E28), A37 (≠ E29), S38 (≠ N30), E59 (= E50), T82 (= T73), F121 (≠ H112), Q122 (= Q113), E123 (≠ Y114), K171 (≠ E162), M266 (≠ L253), V293 (≠ S280), V400 (= V382), G426 (≠ A408), M428 (= M410), D453 (= D435), N480 (≠ D462), H482 (≠ G464), L483 (≠ Y465), M485 (≠ L467), V486 (≠ I468), W489 (= W470), H558 (≠ Y531)
- binding 2-(4-isopropyl-4-methyl-5-oxo-4,5-dihydro-1h-imidazol-2-yl)quinoline-3-carboxylic acid: K135 (= K126), R161 (≠ K152), Y191 (= Y186), R194 (vs. gap), D291 (≠ E278), R292 (≠ Y279), D312 (≠ T298), W489 (= W470)
- binding flavin-adenine dinucleotide: R161 (≠ K152), G222 (= G207), G224 (= G209), T246 (= T233), L247 (≠ F234), M248 (= M235), L264 (≠ V251), G265 (= G252), M266 (≠ L253), H267 (≠ Q254), G286 (= G273), V287 (≠ Y274), R288 (≠ D275), D290 (≠ I277), R292 (≠ Y279), V293 (≠ S280), D310 (≠ G296), I311 (≠ M297), D329 (= D315), V330 (≠ I316), M405 (= M387), G423 (≠ N405), G424 (= G406)
- binding magnesium ion: D453 (= D435), N480 (≠ D462)
- binding thiamine diphosphate: V400 (= V382), G401 (= G383), Q402 (≠ A384), H403 (= H385), G426 (≠ A408), M428 (= M410), G452 (= G434), G454 (= G436), S455 (≠ G437), N480 (≠ D462), H482 (≠ G464), L483 (≠ Y465), G484 (= G466), M485 (≠ L467), V486 (≠ I468)
Sites not aligning to the query:
1yi1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, tribenuron methyl (see paper)
33% identity, 96% coverage: 7:535/550 of query aligns to 15:562/582 of 1yi1A
- active site: Y33 (≠ L25), G35 (= G27), G36 (≠ E28), A37 (≠ E29), S38 (≠ N30), E59 (= E50), T82 (= T73), F121 (≠ H112), Q122 (= Q113), E123 (≠ Y114), K171 (≠ E162), M266 (≠ L253), V293 (≠ S280), V400 (= V382), G426 (≠ A408), M428 (= M410), D453 (= D435), N480 (≠ D462), H482 (≠ G464), L483 (≠ Y465), M485 (≠ L467), V486 (≠ I468), W489 (= W470), H558 (≠ Y531)
- binding methyl 2-[4-methoxy-6-methyl-1,3,5-trazin-2-yl(methyl)carbamoylsulfamoyl]benzoate: D291 (≠ E278), R292 (≠ Y279), W489 (= W470)
- binding flavin-adenine dinucleotide: R161 (≠ K152), G223 (≠ N208), G224 (= G209), T246 (= T233), L247 (≠ F234), M248 (= M235), M263 (≠ T250), L264 (≠ V251), G265 (= G252), M266 (≠ L253), H267 (≠ Q254), G286 (= G273), V287 (≠ Y274), R288 (≠ D275), D290 (≠ I277), V293 (≠ S280), D310 (≠ G296), I311 (≠ M297), D329 (= D315), V330 (≠ I316), M405 (= M387), G423 (≠ N405), G424 (= G406)
- binding magnesium ion: D453 (= D435), N480 (≠ D462), H482 (≠ G464)
Sites not aligning to the query:
1yi0A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, sulfometuron methyl (see paper)
33% identity, 96% coverage: 7:535/550 of query aligns to 15:562/582 of 1yi0A
- active site: Y33 (≠ L25), G35 (= G27), G36 (≠ E28), A37 (≠ E29), S38 (≠ N30), E59 (= E50), T82 (= T73), F121 (≠ H112), Q122 (= Q113), E123 (≠ Y114), K171 (≠ E162), M266 (≠ L253), V293 (≠ S280), V400 (= V382), G426 (≠ A408), M428 (= M410), D453 (= D435), N480 (≠ D462), H482 (≠ G464), L483 (≠ Y465), M485 (≠ L467), V486 (≠ I468), W489 (= W470), H558 (≠ Y531)
- binding methyl 2-[({[(4,6-dimethylpyrimidin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D291 (≠ E278), R292 (≠ Y279), W489 (= W470)
- binding flavin-adenine dinucleotide: R161 (≠ K152), G222 (= G207), G223 (≠ N208), G224 (= G209), T246 (= T233), L247 (≠ F234), M248 (= M235), L264 (≠ V251), G265 (= G252), M266 (≠ L253), H267 (≠ Q254), G286 (= G273), V287 (≠ Y274), R288 (≠ D275), D290 (≠ I277), R292 (≠ Y279), V293 (≠ S280), D310 (≠ G296), I311 (≠ M297), G328 (= G314), D329 (= D315), V330 (≠ I316), M405 (= M387), G423 (≠ N405), G424 (= G406)
- binding magnesium ion: D453 (= D435), N480 (≠ D462), H482 (≠ G464)
Sites not aligning to the query:
1yhzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorsulfuron (see paper)
33% identity, 96% coverage: 7:535/550 of query aligns to 15:562/582 of 1yhzA
- active site: Y33 (≠ L25), G35 (= G27), G36 (≠ E28), A37 (≠ E29), S38 (≠ N30), E59 (= E50), T82 (= T73), F121 (≠ H112), Q122 (= Q113), E123 (≠ Y114), K171 (≠ E162), M266 (≠ L253), V293 (≠ S280), V400 (= V382), G426 (≠ A408), M428 (= M410), D453 (= D435), N480 (≠ D462), H482 (≠ G464), L483 (≠ Y465), M485 (≠ L467), V486 (≠ I468), W489 (= W470), H558 (≠ Y531)
- binding 1-(2-chlorophenylsulfonyl)-3-(4-methoxy-6-methyl-l,3,5-triazin-2-yl)urea: D291 (≠ E278), R292 (≠ Y279), M485 (≠ L467), W489 (= W470)
- binding flavin-adenine dinucleotide: R161 (≠ K152), G223 (≠ N208), G224 (= G209), T246 (= T233), L247 (≠ F234), M248 (= M235), L264 (≠ V251), M266 (≠ L253), H267 (≠ Q254), G286 (= G273), V287 (≠ Y274), R288 (≠ D275), D290 (≠ I277), V293 (≠ S280), D310 (≠ G296), I311 (≠ M297), D329 (= D315), V330 (≠ I316), Q404 (≠ K386), M405 (= M387), G423 (≠ N405), G424 (= G406)
- binding magnesium ion: D453 (= D435), N480 (≠ D462), H482 (≠ G464)
Sites not aligning to the query:
Query Sequence
>WP_009543700.1 NCBI__GCF_000017845.1:WP_009543700.1
MGEMNTAELLVKCLENEGVEYIFGLPGEENLHILEALKDSSIQFITTRHEQGAAFMADVY
GRLTGKAGVCLSTLGPGATNLMTGVADANLDGAPLVAITGQVGTDRMHIESHQYLDLVAM
FAPVTKWNKQIVRPSITPEVTRKAFKLAQSEKPGAVHIDLPENIAALPVEGWPLTRDSQE
KTYASYRSLNAAAMAISKAKNPLILVGNGTIRAHASEALTEFATTLNIPVANTFMGKGAV
PFTHPLSLWTVGLQQRDLITCAFEQSDLVIAVGYDLIEYSPKKWNPEGTTPIIHIGMTPA
EIDSSYIPLVEVVGDISDSLMDILKRADRQGKSTSVAAGLRSDLVREYEKYAHDEGFPVK
PQKIIYDLRQVMGPEDIVISDVGAHKMWMARHYHCDTPNTCLISNGFAAMGIAIPGAIAA
KLVAPDKKVVAVTGDGGFMMNCQELETALRAGTPFVTLIFNDNGYGLIEWKQINQFGQSS
FIKFTNPDFVKFAESMGLKGYRVESADDLVPTLKTALEQGVPAVIDCPVDYQENIRFSQK
AGDLSCQIWE
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory