SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_009543758.1 NCBI__GCF_000017845.1:WP_009543758.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4ij6A Crystal structure of a novel-type phosphoserine phosphatase mutant (h9a) from hydrogenobacter thermophilus tk-6 in complex with l-phosphoserine (see paper)
31% identity, 94% coverage: 3:202/212 of query aligns to 2:200/207 of 4ij6A

query
sites
4ij6A

L

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x
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active site: R8 (= R9), A9 (≠ H10), N15 (≠ S16), R58 (= R59), E82 (= E83), H150 (= H151)
binding phosphoserine: R8 (= R9), Q21 (≠ C22), R58 (= R59), E82 (= E83), H85 (≠ Y86), H150 (= H151), T151 (≠ K152)

1h2fA Bacillus stearothermophilus phoe (previously known as yhfr) in complex with trivanadate (see paper)
29% identity, 93% coverage: 5:202/212 of query aligns to 4:201/207 of 1h2fA

query
sites
1h2fA

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active site: R8 (= R9), H9 (= H10), N15 (≠ S16), R58 (= R59), E82 (= E83), H150 (= H151)
binding phosphate ion: G142 (≠ T143), E143 (≠ G144)
binding trivanadate: R8 (= R9), H9 (= H10), N15 (≠ S16), Q21 (≠ C22), R58 (= R59), E82 (= E83), H150 (= H151), G151 (≠ K152), V152 (≠ A153)

Sites not aligning to the query:

binding phosphate ion: 1, 2, 3

1h2eA Bacillus stearothermophilus phoe (previously known as yhfr) in complex with phosphate (see paper)
29% identity, 93% coverage: 5:202/212 of query aligns to 4:201/207 of 1h2eA

query
sites
1h2eA

L

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active site: R8 (= R9), H9 (= H10), N15 (≠ S16), R58 (= R59), E82 (= E83), H150 (= H151)
binding phosphate ion: R8 (= R9), H9 (= H10), R58 (= R59), E82 (= E83), H150 (= H151)

6m1xC Crystal structure of phosphoserine phosphatase in complex with 3- phosphoglyceric acid from entamoeba histolytica (see paper)
34% identity, 75% coverage: 4:163/212 of query aligns to 3:158/196 of 6m1xC

query
sites
6m1xC

K

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binding 3-phosphoglyceric acid: R8 (= R9), H9 (= H10), Q21 (≠ C22), R57 (= R59), E79 (= E83), H146 (= H151), G147 (≠ K152)

5zr2C Crystal structure of phosphoserine phosphatase mutant (h9a) from entamoeba histolytica in complex with phosphoserine (see paper)
34% identity, 75% coverage: 4:163/212 of query aligns to 3:158/198 of 5zr2C

query
sites
5zr2C

K

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binding phosphoserine: R8 (= R9), Q21 (≠ C22), G22 (= G23), R57 (= R59), E79 (= E83), H146 (= H151), G147 (≠ K152)

P9WIC7 Glucosyl-3-phosphoglycerate phosphatase; Mannosyl-3-phosphoglycerate phosphatase; EC 3.1.3.85; EC 3.1.3.70 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
30% identity, 63% coverage: 4:137/212 of query aligns to 5:137/223 of P9WIC7

query
sites
P9WIC7

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R10 (= R9) mutation to A: Loss of phosphatase activity.
H11 (= H10) active site, Tele-phosphohistidine intermediate; mutation to A: Almost completely abolished phosphatase activity.
N17 (≠ S16) mutation to A: About 5% of wild-type phosphatase activity.
K47 (≠ D46) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
R60 (= R59) mutation to A: Loss of phosphatase activity.

Sites not aligning to the query:

159 H→A: About 5% of wild-type phosphatase activity.
209 L→E: Disrupts dimerization of the enzyme, which exists as a monomer and has lost its ability to perform dephosphorylation.

4pzaB The complex structure of mycobacterial glucosyl-3-phosphoglycerate phosphatase rv2419c with inorganic phosphate (see paper)
30% identity, 63% coverage: 4:137/212 of query aligns to 4:136/217 of 4pzaB

query
sites
4pzaB

K

R

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H

T

G

R

L

L

K

R

E
|
E

I

T

Y

H

Y

L

G

G

E

D

W

W

E

Q

G

G

K

L

T

T

P

H

D

A

E

Q

V

I

N

D

R

A

E

D

F

A

H

P

D

G

D

A

Y

R

V

L

R

A

W

W

L

R

A

E

D

D

P

A

G

T

W

W

N

-

S

A

P

P

N

H

G

G

E

E

K

S

G

R

I

V

D

D

I

V

A

A

R

A

R

R

S

S

S

R

E

P

V

L

L

V

E

A

E

E

I

L

active site: H10 (= H10), R59 (= R59), E83 (= E83)
binding phosphate ion: R9 (= R9), H10 (= H10), R59 (= R59), E83 (= E83)

Sites not aligning to the query:

binding phosphate ion: 158, 159

4qihA The structure of mycobacterial glucosyl-3-phosphoglycerate phosphatase rv2419c complexes with vo3 (see paper)
30% identity, 63% coverage: 4:137/212 of query aligns to 3:135/209 of 4qihA

query
sites
4qihA

K

R

L

L

Y

V

F

M

L

L

R
|
R

H
|
H

G

G

E

Q

T

T

T

D

S

Y

S

N

Q

V

T

G

G

S

T

R

Y

M

C
x
Q

G

G

R

Q

L

L

D

D

I

T

E

E

L

L

T

S

P

E

S

L

G

G

R

R

Q

T

M

Q

A

A

E

V

D

A

F

A

A

A

Q

E

T

V

Y

L

K

G

D

K

L

R

P

Q

W

P

K

L

A

L

V

I

Y

V

C

S

S

S

P

D

L

L

K

R

R
|
R

A

A

I

Y

A

D

T

T

A

A

K

V

P

K

L

L

C

G

D

E

L

R

L

T

N

G

I

L

P

V

M

V

E

R

L

V

R

D

H

T

G

R

L

L

K

R

E
|
E

I

T

Y

H

Y

L

G

G

E

D

W

W

E

Q

G

G

K

L

T

T

P

H

D

A

E

Q

V

I

N

D

R

A

E

D

F

A

H

P

D

G

D

A

Y

R

V

L

R

A

W

W

L

R

A

E

D

D

P

A

G

T

W

W

N

-

S

A

P

P

N

H

G

G

E

E

K

S

G

R

I

V

D

D

I

V

A

A

R

A

R

R

S

S

S

R

E

P

V

L

L

V

E

A

E

E

I

L

active site: H9 (= H10), R58 (= R59), E82 (= E83)
binding vanadate ion: R8 (= R9), H9 (= H10), Q21 (≠ C22), R58 (= R59)

Sites not aligning to the query:

binding vanadate ion: 157

1bifA 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase bifunctional enzyme complexed with atp-g-s and phosphate (see paper)
31% identity, 75% coverage: 5:163/212 of query aligns to 215:366/432 of 1bifA

query
sites
1bifA

L

I

Y

Y

F

L

L

C

R
|
R

H
|
H

G

G

E

E

T

S

T

E

S

L

S
x
N

Q

L

T

K

G

G

T

R

Y

I

C

G

G

G

R

-

L

-

D

D

I

P

E

G

L

L

T

S

P

P

S

R

G

G

R

R

Q

E

M

F

A

S

E

K

D

H

F

L

A

A

Q

Q

T

F

Y

I

K

S

D

D

L

Q

P

N

W

I

K

K

-

D

-

L

A

K

V

V

Y

F

C

T

S

S

P

Q

L

M

K

K

R
|
R

A

T

I

I

A

Q

T

T

A

A

K

-

P

-

L

-

C

-

D

E

L

A

L

L

N

S

I

V

P

P

M

Y

E

E

L

Q

R

F

H

K

G

V

L

L

K

N

E
|
E

I

I

Y

D

Y

A

G

G

E

V

W

C

E

E

G

E

K

M

T

T

P
x
Y

D

E

E

E

V

I

N

Q

R

D

E

H

F

Y

H

P

D

L

D

E

Y

F

V

A

R

L

W
x
R

L

D

A

Q

D

D

P
x
K

G

Y

W

R

N

Y

S

R

P

Y

N

P

G

K

G

G

E

E

K

S

G
x
Y

I

E

D

D

I

L

A

V

R

Q

R

R

S

L

S

E

E

P

V

V

L

I

E

M

E

E

I

L

E

E

D

R

K

Q

Y

-

E

-

T

-

G

E

N

N

V

V

L

L

V

V

V

I

S

C

H
|
H

K

Q

A

A

T

V

I

M

R
|
R

I

C

M

L

L

L

C

A

S

Y

L

F

L

L

active site: R219 (= R9), H220 (= H10), N226 (≠ S16), R269 (= R59), E289 (= E83), H354 (= H151)
binding phosphate ion: R219 (= R9), H220 (= H10), R269 (= R59), E289 (= E83), Y300 (≠ P94), R314 (≠ W108), K318 (≠ P112), Y329 (≠ G123), H354 (= H151), R359 (= R156)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 11, 12, 13, 14, 15, 16, 17, 131, 134, 136, 184, 210, 391

P25114 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4; 6PF-2-K/Fru-2,6-P2ase 4; PFK/FBPase 4; 6PF-2-K/Fru-2,6-P2ase testis-type isozyme; EC 2.7.1.105; EC 3.1.3.46 from Rattus norvegicus (Rat) (see 2 papers)
31% identity, 75% coverage: 5:163/212 of query aligns to 252:403/469 of P25114

query
sites
P25114

L

I

Y

Y

F

L

L

C

R

R

H
|
H

G

G

E

E

T

S

T

E

S

L

S

N

Q

L

T

K

G

G

T

R

Y

I

C
x
G

G

G

R

-

L

-

D

D

I

P

E

G

L

L

T

S

P

P

S

R

G

G

R

R

Q

E

M

F

A

S

E

K

D

H

F

L

A

A

Q

Q

T

F

Y

I

K

S

D

D

L

Q

P

N

W

I

K

K

-

D

-

L

A

K

V

V

Y

W

C

T

S

S

P

Q

L

M

K

K

R

R

A

T

I

I

A

Q

T

T

A

A

K

-

P

-

L

-

C

-

D

E

L

A

L

L

N

S

I

V

P

P

M

Y

E

E

L

Q

R

W

H

K

G

V

L

L

K

N

E
|
E

I

I

Y

D

Y

A

G

G

E

V

W

C

E

E

G

E

K

M

T

T

P
x
Y

D

E

E

E

V

I

N

Q

R

D

E

H

F

Y

H

P

D

L

D

E

Y

F

V

A

R

L

W
x
R

L

D

A

Q

D

D

P
x
K

G

Y

W

R

N

Y

S

R

P

Y

N

P

G

K

G

G

E

E

K

S

G
x
Y

I

E

D

D

I

L

A

V

R

Q

R

R

S

L

S

E

E

P

V

V

L

I

E

M

E

E

I

L

E

E

D

R

K

Q

Y

-

E

-

T

-

G

E

N

N

V

V

L

L

V

V

V

I

S

C

H

H

K
x
Q

A

A

T

V

I

M

R
|
R

I

C

M

L

L

L

C

A

S

Y

L

F

L

L

H257 (= H10) active site, Tele-phosphohistidine intermediate
G269 (≠ C22) binding beta-D-fructose 2,6-bisphosphate
E326 (= E83) active site, Proton donor/acceptor
Y337 (≠ P94) binding beta-D-fructose 2,6-bisphosphate
R351 (≠ W108) binding beta-D-fructose 2,6-bisphosphate
K355 (≠ P112) binding beta-D-fructose 2,6-bisphosphate
Y366 (≠ G123) binding beta-D-fructose 2,6-bisphosphate
Q392 (≠ K152) binding beta-D-fructose 2,6-bisphosphate
R396 (= R156) binding beta-D-fructose 2,6-bisphosphate

Sites not aligning to the query:

46:54 binding ATP
79 binding beta-D-fructose 6-phosphate
131 binding beta-D-fructose 6-phosphate
137 binding beta-D-fructose 6-phosphate
168:173 binding ATP
198 binding beta-D-fructose 6-phosphate
428 binding ATP

6ic0A Human pfkfb3 in complex with a n-aryl 6-aminoquinoxaline inhibitor 4 (see paper)
30% identity, 75% coverage: 5:163/212 of query aligns to 231:382/428 of 6ic0A

query
sites
6ic0A

L

I

Y

Y

F

L

L

C

R

R

H

H

G

G

E

E

T

N

T

E

S

H

S

N

Q

L

T

Q

G

G

T

R

Y

I

C
x
G

G

G

R

-

L

-

D

D

I

S

E

G

L

L

T

S

P

S

S

R

G

G

R

K

Q

K

M

F

A

A

E

S

D

A

F

L

A

S

-

K

-

F

-

V

-

E

Q

Q

T

N

Y

L

K

K

D

D

L

L

P

-

W

-

K

-

A

R

V

V

Y

W

C

T

S

S

P

Q

L

L

K

K

R

S

A

T

I

I

A

Q

T

T

A

A

K

-

P

-

L

-

C

-

D

E

L

A

L

L

N

R

I

L

P

P

M

Y

E

E

L

Q

R

W

H

K

G

A

L

L

K

N

E
|
E

I

I

Y

D

Y

A

G

G

E

V

W

C

E

E

G

E

K

L

T

T

P
x
Y

D

E

E

E

V

I

N

R

R

D

E

T

F

Y

H

P

D

E

Y

Y

V

A

R

L

W
x
R

L

E

A

Q

D

D

P
x
K

G

Y

W

Y

N

Y

S

R

P

Y

N

P

G

T

G

G

E

E

K

S

G
x
Y

I

Q

D

D

I

L

A

V

R

Q

R

R

S

L

S

E

E

P

V

V

L

I

E

M

E

E

I

L

E

E

D

R

K

Q

Y

-

E

-

T

-

G

E

N

N

V

V

L

L

V

V

V

I

S

C

H

H

K
x
Q

A

A

T

V

I

L

R
|
R

I

C

M

L

L

L

C

A

S

Y

L

F

L

L

binding 6-O-phosphono-beta-D-fructofuranose: G248 (≠ C22), E305 (= E83), Y316 (≠ P94), R330 (≠ W108), K334 (≠ P112), Y345 (≠ G123), Q371 (≠ K152), R375 (= R156)

Sites not aligning to the query:

binding 3-[[8-(1-methylindol-6-yl)quinoxalin-6-yl]amino]-~{N}-pyrimidin-5-yl-pyridine-4-carboxamide: 28, 29, 32, 33, 135, 137, 146, 149, 197, 200, 224, 226, 404
binding pyrophosphate 2-: 26, 27, 28, 29, 30, 31, 32, 151, 407

6etjA Human pfkfb3 in complex with kan0438241 (see paper)
30% identity, 75% coverage: 5:163/212 of query aligns to 236:387/432 of 6etjA

query
sites
6etjA

L

I

Y

Y

F

L

L

C

R
|
R

H
|
H

G

G

E

E

T

N

T

E

S

H

S
x
N

Q

L

T

Q

G

G

T

R

Y
x
I

C
x
G

G

G

R

-

L

-

D

D

I

S

E

G

L

L

T

S

P

S

S

R

G

G

R

K

Q

K

M

F

A

A

E

S

D

A

F

L

A

S

-

K

-

F

-

V

-

E

Q

Q

T

N

Y

L

K

K

D

D

L

L

P

-

W

-

K

-

A

R

V

V

Y

W

C

T

S

S

P

Q

L

L

K

K

R
x
S

A

T

I

I

A

Q

T

T

A

A

K

-

P

-

L

-

C

-

D

E

L

A

L

L

N

R

I

L

P

P

M

Y

E

E

L

Q

R

W

H

K

G

A

L

L

K

N

E
|
E

I

I

Y

D

Y

A

G

G

E

V

W

C

E

E

G

E

K

L

T

T

P
x
Y

D

E

E

E

V

I

N

R

R

D

E

T

F

Y

H

P

D

E

Y

Y

V

A

R

L

W
x
R

L

E

A

Q

D

D

P
x
K

G

Y

W

Y

N

Y

S

R

P

Y

N

P

G

T

G

G

E

E

K

S

G
x
Y

I

Q

D

D

I

L

A

V

R

Q

R

R

S

L

S

E

E

P

V

V

L

I

E

M

E

E

I

L

E

E

D

R

K

Q

Y

-

E

-

T

-

G

E

N

N

V

V

L

L

V

V

V

I

S

C

H
|
H

K
x
Q

A

A

T

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R
|
R

I

C

M

L

L

L

C

A

S

Y

L

F

L

L

active site: R240 (= R9), H241 (= H10), N247 (≠ S16), S290 (≠ R59), E310 (= E83), H375 (= H151)
binding 6-O-phosphono-beta-D-fructofuranose: H241 (= H10), I252 (≠ Y21), G253 (≠ C22), E310 (= E83), Y321 (≠ P94), R335 (≠ W108), K339 (≠ P112), Y350 (≠ G123), Q376 (≠ K152), R380 (= R156)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 32, 33, 34, 35, 36, 37, 151, 154, 155, 156, 205, 412
binding 4-[[3-(5-fluoranyl-2-oxidanyl-phenyl)phenyl]sulfonylamino]-2-oxidanyl-benzoic acid: 58, 59, 62, 75, 83, 86, 113, 114, 115, 120, 181

2dwpA A pseudo substrate complex of 6-phosphofructo-2-kinase of pfkfb (see paper)
30% identity, 75% coverage: 5:163/212 of query aligns to 234:385/431 of 2dwpA

query
sites
2dwpA

L

I

Y

Y

F

L

L

C

R
|
R

H
|
H

G

G

E

E

T

N

T

E

S

H

S
x
N

Q

L

T

Q

G

G

T

R

Y
x
I

C
x
G

G

G

R

-

L

-

D

D

I

S

E

G

L

L

T

S

P

S

S

R

G

G

R

K

Q

K

M

F

A

A

E

S

D

A

F

L

A

S

-

K

-

F

-

V

-

E

Q

Q

T

N

Y

L

K

K

D

D

L

L

P

-

W

-

K

-

A

R

V

V

Y

W

C

T

S

S

P

Q

L

L

K

K

R
x
S

A

T

I

I

A

Q

T

T

A

A

K

-

P

-

L

-

C

-

D

E

L

A

L

L

N

R

I

L

P

P

M

Y

E

E

L

Q

R

W

H

K

G

A

L

L

K

N

E
|
E

I

I

Y

D

Y

A

G

G

E

V

W

C

E

E

G

E

K

L

T

T

P
x
Y

D

E

E

E

V

I

N

R

R

D

E

T

F

Y

H

P

D

E

Y

Y

V

A

R

L

W
x
R

L

E

A

Q

D

D

P
x
K

G

Y

W

Y

N

Y

S

R

P

Y

N

P

G

T

G

G

E

E

K

S

G
x
Y

I

Q

D

D

I

L

A

V

R

Q

R

R

S

L

S

E

E

P

V

V

L

I

E

M

E

E

I

L

E

E

D

R

K

Q

Y

-

E

-

T

-

G

E

N

N

V

V

L

L

V

V

V

I

S

C

H
|
H

K
x
Q

A

A

T

V

I

L

R
|
R

I

C

M

L

L

L

C

A

S

Y

L

F

L

L

active site: R238 (= R9), H239 (= H10), N245 (≠ S16), S288 (≠ R59), E308 (= E83), H373 (= H151)
binding 6-O-phosphono-beta-D-fructofuranose: I250 (≠ Y21), G251 (≠ C22), E308 (= E83), Y319 (≠ P94), R333 (≠ W108), K337 (≠ P112), Y348 (≠ G123), Q374 (≠ K152), R378 (= R156)

Sites not aligning to the query:

binding phosphomethylphosphonic acid adenylate ester: 29, 30, 31, 32, 33, 34, 35, 138, 149, 152, 153, 154, 203, 410
binding 6-O-phosphono-beta-D-fructofuranose: 57, 60, 111, 112, 118, 175, 179, 426

6hvjA Human pfkfb3 in complex with a n-aryl 6-aminoquinoxaline inhibitor 3 (see paper)
30% identity, 75% coverage: 5:163/212 of query aligns to 232:383/430 of 6hvjA

query
sites
6hvjA

L

I

Y

Y

F

L

L

C

R
|
R

H
|
H

G

G

E

E

T

N

T

E

S

H

S
x
N

Q

L

T

Q

G

G

T

R

Y

I

C
x
G

G

G

R

-

L

-

D

D

I

S

E

G

L

L

T

S

P

S

S

R

G

G

R

K

Q

K

M

F

A

A

E

S

D

A

F

L

A

S

-

K

-

F

-

V

-

E

Q

Q

T

N

Y

L

K

K

D

D

L

L

P

-

W

-

K

-

A

R

V

V

Y

W

C

T

S

S

P

Q

L

L

K

K

R
x
S

A

T

I

I

A

Q

T

T

A

A

K

-

P

-

L

-

C

-

D

E

L

A

L

L

N

R

I

L

P

P

M

Y

E

E

L

Q

R

W

H

K

G

A

L

L

K

N

E
|
E

I

I

Y

D

Y

A

G

G

E

V

W

C

E

E

G

E

K

L

T

T

P
x
Y

D

E

E

E

V

I

N

R

R

D

E

T

F

Y

H

P

D

E

Y

Y

V

A

R

L

W
x
R

L

E

A

Q

D

D

P
x
K

G

Y

W

Y

N

Y

S

R

P

Y

N

P

G

T

G

G

E

E

K

S

G
x
Y

I

Q

D

D

I

L

A

V

R

Q

R

R

S

L

S

E

E

P

V

V

L

I

E

M

E

E

I

L

E

E

D

R

K

Q

Y

-

E

-

T

-

G

E

N

N

V

V

L

L

V

V

V

I

S

C

H
|
H

K
x
Q

A

A

T

V

I

L

R
|
R

I

C

M

L

L

L

C

A

S

Y

L

F

L

L

active site: R236 (= R9), H237 (= H10), N243 (≠ S16), S286 (≠ R59), E306 (= E83), H371 (= H151)
binding 6-O-phosphono-beta-D-fructofuranose: G249 (≠ C22), E306 (= E83), Y317 (≠ P94), R331 (≠ W108), K335 (≠ P112), Y346 (≠ G123), Q372 (≠ K152), R376 (= R156)

Sites not aligning to the query:

binding 8-(3-methyl-1-benzofuran-5-yl)-~{N}-(4-methylsulfonylpyridin-3-yl)quinoxalin-6-amine: 29, 33, 34, 136, 138, 143, 147, 151, 198, 225, 407
binding phosphate ion: 28, 29, 30, 31, 32, 152

6ibxA Human pfkfb3 in complex with a n-aryl 6-aminoquinoxaline inhibitor 5 (see paper)
30% identity, 75% coverage: 5:163/212 of query aligns to 231:382/429 of 6ibxA

query
sites
6ibxA

L

I

Y

Y

F

L

L

C

R

R

H

H

G

G

E

E

T

N

T

E

S

H

S

N

Q

L

T

Q

G

G

T

R

Y

I

C
x
G

G

G

R

-

L

-

D

D

I

S

E

G

L

L

T

S

P

S

S

R

G

G

R

K

Q

K

M

F

A

A

E

S

D

A

F

L

A

S

-

K

-

F

-

V

-

E

Q

Q

T

N

Y

L

K

K

D

D

L

L

P

-

W

-

K

-

A

R

V

V

Y

W

C

T

S

S

P

Q

L

L

K

K

R

S

A

T

I

I

A

Q

T

T

A

A

K

-

P

-

L

-

C

-

D

E

L

A

L

L

N

R

I

L

P

P

M

Y

E

E

L

Q

R

W

H

K

G

A

L

L

K

N

E
|
E

I

I

Y

D

Y

A

G

G

E

V

W

C

E

E

G

E

K

L

T

T

P
x
Y

D

E

E

E

V

I

N

R

R

D

E

T

F

Y

H

P

D

E

Y

Y

V

A

R

L

W
x
R

L

E

A

Q

D

D

P
x
K

G

Y

W

Y

N

Y

S

R

P

Y

N

P

G

T

G

G

E

E

K

S

G
x
Y

I

Q

D

D

I

L

A

V

R

Q

R

R

S

L

S

E

E

P

V

V

L

I

E

M

E

E

I

L

E

E

D

R

K

Q

Y

-

E

-

T

-

G

E

N

N

V

V

L

L

V

V

V

I

S

C

H

H

K
x
Q

A

A

T

V

I

L

R
|
R

I

C

M

L

L

L

C

A

S

Y

L

F

L

L

binding 6-O-phosphono-beta-D-fructofuranose: G248 (≠ C22), E305 (= E83), Y316 (≠ P94), R330 (≠ W108), K334 (≠ P112), Y345 (≠ G123), Q371 (≠ K152), R375 (= R156)

Sites not aligning to the query:

binding 3-[[8-(1-methylindol-6-yl)quinoxalin-6-yl]amino]-~{N}-[(3~{S})-1-methylpiperidin-3-yl]pyridine-4-carboxamide: 28, 32, 33, 135, 137, 142, 146, 149, 150, 197, 224
binding pyrophosphate 2-: 27, 28, 29, 30, 31, 32, 151, 407

3qpuA Pfkfb3 in complex with ppi (see paper)
30% identity, 75% coverage: 5:163/212 of query aligns to 241:392/439 of 3qpuA

query
sites
3qpuA

L

I

Y

Y

F

L

L

C

R
|
R

H
|
H

G

G

E

E

T

N

T

E

S

H

S
x
N

Q

L

T

Q

G

G

T

R

Y

I

C

G

G

G

R

-

L

-

D

D

I

S

E

G

L

L

T

S

P

S

S

R

G

G

R

K

Q

K

M

F

A

A

E

S

D

A

F

L

A

S

-

K

-

F

-

V

-

E

Q

Q

T

N

Y

L

K

K

D

D

L

L

P

-

W

-

K

-

A

R

V

V

Y

W

C

T

S

S

P

Q

L

L

K

K

R
x
S

A

T

I

I

A

Q

T

T

A

A

K

-

P

-

L

-

C

-

D

E

L

A

L

L

N

R

I

L

P

P

M

Y

E

E

L

Q

R

W

H

K

G

A

L

L

K

N

E
|
E

I

I

Y

D

Y

A

G

G

E

V

W

C

E

E

G

E

K

L

T

T

P

Y

D

E

E

E

V

I

N

R

R

D

E

T

F

Y

H

P

D

E

Y

Y

V

A

R

L

W

R

L

E

A

Q

D

D

P

K

G

Y

W

Y

N

Y

S

R

P

Y

N

P

G

T

G

G

E

E

K

S

G

Y

I

Q

D

D

I

L

A

V

R

Q

R

R

S

L

S

E

E

P

V

V

L

I

E

M

E

E

I

L

E

E

D

R

K

Q

Y

-

E

-

T

-

G

E

N

N

V

V

L

L

V

V

V

I

S

C

H
|
H

K
x
Q

A

A

T

V

I

L

R

R

I

C

M

L

L

L

C

A

S

Y

L

F

L

L

active site: R245 (= R9), H246 (= H10), N252 (≠ S16), S295 (≠ R59), E315 (= E83), H380 (= H151)
binding pyrophosphate 2-: R245 (= R9), H246 (= H10), E315 (= E83), H380 (= H151), Q381 (≠ K152)

Sites not aligning to the query:

binding pyrophosphate 2-: 36, 37, 38, 39, 40, 41, 42, 64, 67, 91, 119, 160, 161, 186, 417

6ibzA Human pfkfb3 in complex with a n-aryl 6-aminoquinoxaline inhibitor 7 (see paper)
30% identity, 75% coverage: 5:163/212 of query aligns to 233:384/431 of 6ibzA

query
sites
6ibzA

L

I

Y

Y

F

L

L

C

R

R

H

H

G

G

E

E

T

N

T

E

S

H

S

N

Q

L

T

Q

G

G

T

R

Y

I

C
x
G

G

G

R

-

L

-

D

D

I

S

E

G

L

L

T

S

P

S

S

R

G

G

R

K

Q

K

M

F

A

A

E

S

D

A

F

L

A

S

-

K

-

F

-

V

-

E

Q

Q

T

N

Y

L

K

K

D

D

L

L

P

-

W

-

K

-

A

R

V

V

Y

W

C

T

S

S

P

Q

L

L

K

K

R

S

A

T

I

I

A

Q

T

T

A

A

K

-

P

-

L

-

C

-

D

E

L

A

L

L

N

R

I

L

P

P

M

Y

E

E

L

Q

R

W

H

K

G

A

L

L

K

N

E
|
E

I

I

Y

D

Y

A

G

G

E

V

W

C

E

E

G

E

K

L

T

T

P
x
Y

D

E

E

E

V

I

N

R

R

D

E

T

F

Y

H

P

D

E

Y

Y

V

A

R

L

W
x
R

L

E

A

Q

D

D

P
x
K

G

Y

W

Y

N

Y

S

R

P

Y

N

P

G

T

G

G

E

E

K

S

G
x
Y

I

Q

D

D

I

L

A

V

R

Q

R

R

S

L

S

E

E

P

V

V

L

I

E

M

E

E

I

L

E

E

D

R

K

Q

Y

-

E

-

T

-

G

E

N

N

V

V

L

L

V

V

V

I

S

C

H

H

K
x
Q

A

A

T

V

I

L

R
|
R

I

C

M

L

L

L

C

A

S

Y

L

F

L

L

binding 6-O-phosphono-beta-D-fructofuranose: G250 (≠ C22), E307 (= E83), Y318 (≠ P94), R332 (≠ W108), K336 (≠ P112), Y347 (≠ G123), Q373 (≠ K152), R377 (= R156)

Sites not aligning to the query:

binding 2-[[8-(1-methylindol-6-yl)quinoxalin-6-yl]amino]-~{N}-(pyrimidin-5-ylmethyl)benzenesulfonamide: 30, 34, 35, 137, 139, 144, 148, 152, 199, 202, 226, 228
binding phosphate ion: 28, 29, 30, 31, 32, 33, 153

6hvhA Human pfkfb3 in complex with a n-aryl 6-aminoquinoxaline inhibitor 1 (see paper)
30% identity, 75% coverage: 5:163/212 of query aligns to 233:384/431 of 6hvhA

query
sites
6hvhA

L

I

Y

Y

F

L

L

C

R
|
R

H
|
H

G

G

E

E

T

N

T

E

S

H

S
x
N

Q

L

T

Q

G

G

T

R

Y
x
I

C
x
G

G

G

R

-

L

-

D

D

I

S

E

G

L

L

T

S

P

S

S

R

G

G

R

K

Q

K

M

F

A

A

E

S

D

A

F

L

A

S

-

K

-

F

-

V

-

E

Q

Q

T

N

Y

L

K

K

D

D

L

L

P

-

W

-

K

-

A

R

V

V

Y

W

C

T

S

S

P

Q

L

L

K

K

R
x
S

A

T

I

I

A

Q

T

T

A

A

K

-

P

-

L

-

C

-

D

E

L

A

L

L

N

R

I

L

P

P

M

Y

E

E

L

Q

R

W

H

K

G

A

L

L

K

N

E
|
E

I

I

Y

D

Y

A

G

G

E

V

W

C

E

E

G

E

K

L

T

T

P
x
Y

D

E

E

E

V

I

N

R

R

D

E

T

F

Y

H

P

D

E

Y

Y

V

A

R

L

W
x
R

L

E

A

Q

D

D

P
x
K

G

Y

W

Y

N

Y

S

R

P

Y

N

P

G

T

G

G

E

E

K

S

G
x
Y

I

Q

D

D

I

L

A

V

R

Q

R

R

S

L

S

E

E

P

V

V

L

I

E

M

E

E

I

L

E

E

D

R

K

Q

Y

-

E

-

T

-

G

E

N

N

V

V

L

L

V

V

V

I

S

C

H
|
H

K
x
Q

A

A

T

V

I

L

R
|
R

I

C

M

L

L

L

C

A

S

Y

L

F

L

L

active site: R237 (= R9), H238 (= H10), N244 (≠ S16), S287 (≠ R59), E307 (= E83), H372 (= H151)
binding 6-O-phosphono-beta-D-fructofuranose: I249 (≠ Y21), G250 (≠ C22), E307 (= E83), Y318 (≠ P94), R332 (≠ W108), K336 (≠ P112), Y347 (≠ G123), Q373 (≠ K152), R377 (= R156)

Sites not aligning to the query:

binding 3-[[8-(1-methylindol-6-yl)quinoxalin-6-yl]amino]-~{N}-(oxan-4-yl)pyridine-4-carboxamide: 30, 34, 35, 137, 139, 144, 148, 151, 152, 199, 202, 226, 228
binding pyrophosphate 2-: 29, 30, 31, 32, 33, 34, 153, 409

2i1vB Crystal structure of pfkfb3 in complex with adp and fructose-2,6- bisphosphate (see paper)
30% identity, 75% coverage: 5:163/212 of query aligns to 244:395/449 of 2i1vB

query
sites
2i1vB

L

I

Y

Y

F

L

L

C

R
|
R

H
|
H

G

G

E

E

T

N

T

E

S

H

S
x
N

Q

L

T

Q

G

G

T

R

Y
x
I

C
x
G

G

G

R

-

L

-

D

D

I

S

E

G

L

L

T

S

P

S

S

R

G

G

R

K

Q

K

M

F

A

A

E

S

D

A

F

L

A

S

-

K

-

F

-

V

-

E

Q

Q

T

N

Y

L

K

K

D

D

L

L

P

-

W

-

K

-

A

R

V

V

Y

W

C

T

S

S

P

Q

L

L

K

K

R

S

A

T

I

I

A

Q

T

T

A

A

K

-

P

-

L

-

C

-

D

E

L

A

L

L

N

R

I

L

P

P

M

Y

E

E

L

Q

R

W

H

K

G

A

L

L

K

N

E
|
E

I

I

Y

D

Y

A

G

G

E

V

W

C

E

E

G

E

K

L

T

T

P
x
Y

D

E

E

E

V

I

N

R

R

D

E

T

F

Y

H

P

D

E

Y

Y

V

A

R

L

W
x
R

L

E

A

Q

D

D

P
x
K

G

Y

W

Y

N

Y

S

R

P

Y

N

P

G

T

G

G

E

E

K

S

G
x
Y

I

Q

D

D

I

L

A

V

R

Q

R

R

S

L

S

E

E

P

V

V

L

I

E

M

E

E

I

L

E

E

D

R

K

Q

Y

-

E

-

T

-

G

E

N

N

V

V

L

L

V

V

V

I

S

C

H
|
H

K
x
Q

A

A

T

V

I

L

R
|
R

I

C

M

L

L

L

C

A

S

Y

L

F

L

L

binding 6-O-phosphono-beta-D-fructofuranose: I260 (≠ Y21), G261 (≠ C22), E318 (= E83), Y329 (≠ P94), R343 (≠ W108), K347 (≠ P112), Y358 (≠ G123), Q384 (≠ K152), R388 (= R156)
binding phosphonic acid: R248 (= R9), H249 (= H10), N255 (≠ S16), H383 (= H151)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 39, 40, 41, 42, 43, 44, 45, 159, 163, 164, 213, 420
binding 2,6-di-O-phosphono-beta-D-fructofuranose: 67, 70, 83, 121, 122, 128, 185, 189

2dwoA Pfkfb3 in complex with adp and pep (see paper)
30% identity, 75% coverage: 5:163/212 of query aligns to 244:395/449 of 2dwoA

query
sites
2dwoA

L

I

Y

Y

F

L

L

C

R
|
R

H
|
H

G

G

E

E

T

N

T

E

S

H

S
x
N

Q

L

T

Q

G

G

T

R

Y
x
I

C
x
G

G

G

R

-

L

-

D

D

I

S

E

G

L

L

T

S

P

S

S

R

G

G

R

K

Q

K

M

F

A

A

E

S

D

A

F

L

A

S

-

K

-

F

-

V

-

E

Q

Q

T

N

Y

L

K

K

D

D

L

L

P

-

W

-

K

-

A

R

V

V

Y

W

C

T

S

S

P

Q

L

L

K

K

R
x
S

A

T

I

I

A

Q

T

T

A

A

K

-

P

-

L

-

C

-

D

E

L

A

L

L

N

R

I

L

P

P

M

Y

E

E

L

Q

R

W

H

K

G

A

L

L

K

N

E
|
E

I

I

Y

D

Y

A

G

G

E

V

W

C

E

E

G

E

K

L

T

T

P
x
Y

D

E

E

E

V

I

N

R

R

D

E

T

F

Y

H

P

D

E

Y

Y

V

A

R

L

W
x
R

L

E

A

Q

D

D

P
x
K

G

Y

W

Y

N

Y

S

R

P

Y

N

P

G

T

G

G

E

E

K

S

G
x
Y

I

Q

D

D

I

L

A

V

R

Q

R

R

S

L

S

E

E

P

V

V

L

I

E

M

E

E

I

L

E

E

D

R

K

Q

Y

-

E

-

T

-

G

E

N

N

V

V

L

L

V

V

V

I

S

C

H
|
H

K
x
Q

A

A

T

V

I

L

R
|
R

I

C

M

L

L

L

C

A

S

Y

L

F

L

L

active site: R248 (= R9), H249 (= H10), N255 (≠ S16), S298 (≠ R59), E318 (= E83), H383 (= H151)
binding 6-O-phosphono-beta-D-fructofuranose: I260 (≠ Y21), G261 (≠ C22), E318 (= E83), Y329 (≠ P94), R343 (≠ W108), K347 (≠ P112), Y358 (≠ G123), Q384 (≠ K152), R388 (= R156)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 39, 40, 41, 42, 43, 44, 45, 148, 159, 162, 163, 164, 239, 420
binding 6-O-phosphono-beta-D-fructofuranose: 436
binding phosphoenolpyruvate: 70, 83, 94, 122, 189

Query Sequence

>WP_009543758.1 NCBI__GCF_000017845.1:WP_009543758.1
MSLKLYFLRHGETTSSQTGTYCGRLDIELTPSGRQMAEDFAQTYKDLPWKAVYCSPLKRA
IATAKPLCDLLNIPMELRHGLKEIYYGEWEGKTPDEVNREFHDDYVRWLADPGWNSPNGG
EKGIDIARRSSEVLEEIEDKYETGNVLVVSHKATIRIMLCSLLGIDVGRFRDRIGMAVAA
VSVVEMAEHGPLVHIMGDRSHIRTELRERQGT

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory