SitesBLAST

M1 (= M1) modified: Initiator methionine, Removed
C2 (= C2) mutation C->A,S: Loss of glutamine-dependent activity but no effect on ammonia-dependent asparagine synthetase activity.
H30 (= H25) mutation to A: 4,5-fold decrease in glutamine affinity.
D34 (= D29) mutation D->N,E: Little effect on the kinetic properties.
H81 (≠ Y77) mutation to A: 5-fold decrease in glutamine affinity.
A105 (≠ H101) mutation to H: Little effect on the kinetic properties.
E349 (= E326) mutation E->A,Q: Loss of glutamine- and ammonia-dependent synthetase activity, but still exhibits glutaminase activity.; mutation to D: 5-fold increase in affinity for aspartate when assaying both the glutamine- and ammonia-dependent synthetase reactions, and 2-fold decrease in kcat for these reactions. Modifies the product glutamate/asparagine stoichiometry.

P78753 Probable asparagine synthetase [glutamine-hydrolyzing]; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
38% identity, 99% coverage: 1:497/501 of query aligns to 1:535/557 of P78753

query
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P78753

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S391 (≠ I361) modified: Phosphoserine
S489 (= S449) modified: Phosphoserine

1ct9A Crystal structure of asparagine synthetase b from escherichia coli (see paper)
40% identity, 92% coverage: 25:486/501 of query aligns to 29:491/497 of 1ct9A

query
sites
1ct9A

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A

active site: L50 (= L48), N74 (= N71), G75 (= G72), T305 (≠ D300), R308 (= R303), E332 (= E326), M366 (≠ N360)
binding adenosine monophosphate: L232 (≠ F225), L233 (= L226), S234 (= S227), S239 (= S232), A255 (≠ S250), V256 (= V251), D263 (= D258), M316 (≠ T311), S330 (≠ T324), G331 (= G325), E332 (= E326)
binding glutamine: R49 (= R47), L50 (= L48), I52 (= I50), V53 (≠ M51), N74 (= N71), G75 (= G72), E76 (= E73), D98 (= D95)

Sites not aligning to the query:

active site: 1
binding glutamine: 1

P08243 Asparagine synthetase [glutamine-hydrolyzing]; Cell cycle control protein TS11; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Homo sapiens (Human) (see 7 papers)
33% identity, 95% coverage: 1:477/501 of query aligns to 1:522/561 of P08243

query
sites
P08243

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N

I

V

V

E

K

K

-

L

-

F

-

P

-

G

-

F

E

E

I

I

Q

-

E

E

T

T

V

V

Q

K

-

N

-

L

-

R

-

I

-

L

-

F

-

N

A

N

S

A

V

V

V

K

K

K

R

R

L

L

M

M

A

T

D

D

V

R

P

R

L

I

G

G

A

C

F

L

L

L

S

S

G

G

L

L

D

D

S

S

I

L

I

V

A

A

I

T

A

L

K

L

K

K

H

Q

K

L

S

K

E

E

-

A

-

Q

-

V

-

Q

-

Y

-

P

L

L

H

Q

T

T

F

F

S

A

V

I

G

G

V

M

E

E

G

D

S

S

K

P

D

D

I

L

N

L

A

A

R

R

L

K

V

V

S

A

D

D

Y

H

L

I

G

G

T

S

I

E

H

H

H

Y

E

E

Y

V

L

L

I

F

T

N

P

S

K

E

E

E

A

G

I

I

A

Q

K

A

L

L

P

D

E

E

I

V

I

I

Y

F

A

S

L

L

E

E

S

T

F

Y

D

D

Q

I

D

T

L

T

V

V

R

R

S

A

A

S

I

V

P

G

C

M

Y

Y

F

L

T

I

S

S

R

K

L

Y

A

I

A

R

Q

K

Y

N

V

T

K

D

-

S

-

V

V

V

I

I

L
x
F

T

S

G

G

E

E

G

G

A

S

D

D

E

E

L

L

F

T

A

Q

G

G

Y

Y

T

I

Y

Y

Y

F

K

H

D

K

I

A

T

P

S

S

D

P

A

E

I

K

L

A

H

E

T

E

E

E

L

S

R

E

R

R

S

L

V

L

S

R

S

E

L

L

H

Y

N

L

I

F

N

D

L

V

Q

L

R

R

V

A

D

D

R

R

L

T

T

T

M

A

A

A

H

H

S

G

I

L

E

E

G

L

R

R

V

V

P

P

F

F

L

L

D

D

L

H

K

R

M

F

I

S

E

S

L

Y

G

Y

Q

L

K

S

I

L

P

P

A

P

H

E

L

M

K

R

L

I

R

P

G

K

N

N

P

-

P

G

V

I

E

E

K

K

W

H

I

L

L

L

R

R

K

E

A

T

F

F

E

E

D

D

-

S

-

N

I

L

L

I

P

P

D

K

E

E

I

I

V

L

W

W

R

R

K

P

K

K

E

E

Q

A

F

F

D

S

E

D

G

G

S

I

G

T

T

S

V

V

-

K

-

N

-

S

-

W

-

F

-

K

-

I

-

L

-

Q

D

E

L

Y

L

V

V

E

D

H

T

Q

L

V

K

D

D

D

I

A

M

M

S

M

E

A

E

N

Q

A

A

A

Q

Q

T

K

Y

F

Q

P

K

F

Q

N

H

-

S

-

H

-

I

-

N

T

L

P

R

K

S

T

A

K

E

E

E

G

C

Y

Y

Y

H

R

Q

Q

I

V

F

F

C2 (= C2) active site, For GATase activity; mutation to A: Loss of the glutamine-dependent asparagine synthetase activity, while the ammonia-dependent activity remained unaffected.
A6 (≠ G6) to E: in ASNSD; dramatic reduction in protein abundance; dbSNP:rs398122975
V210 (vs. gap) to E: in dbSNP:rs1049674
F362 (≠ L323) to V: in ASNSD; dramatic reduction in protein abundance; dbSNP:rs398122973

Sites not aligning to the query:

550 R → C: in ASNSD; increases level of protein abundance; dbSNP:rs398122974

6gq3A Human asparagine synthetase (asns) in complex with 6-diazo-5-oxo-l- norleucine (don) at 1.85 a resolution (see paper)
34% identity, 95% coverage: 2:477/501 of query aligns to 1:496/509 of 6gq3A

query
sites
6gq3A

C
|
C

G

G

I

I

A
x
W

G

A

I

L

W

F

G

G

K

S

T

D

S

D

Q

C

S

L

N

S

I

V

E

Q

A

C

M

L

M

S

E

A

-

M

S

K

I

I

V

A

H

H

R

R

G

G

P

P

D

D

A

A

N

-

G

-

I

-

F

F

V

R

V

F

P

E

D

N

G

V

S

N

G

G

-

Y

-

T

-

N

-

C

I

C

L

F

G

G

H

F

Q

H

R
|
R

L
|
L

S

A

I
x
V

M
x
V

D

D

V

P

E

L

G

F

G

G

D

M

Q

Q

P

P

I

I

Y

-

G

-

D

R

G

V

K

K

A

Y

-

P

-

Y

-

L

-

W

I

L

I

C

G
x
Y

N
|
N

G
|
G

E
|
E

I

I

Y

Y

N

N

Y

H

P

K

Q

K

L

M

F

Q

S

Q

D

H

L

F

E

E

S

F

K

E

Y

Y

Q

Q

F

-

V

-

T

T

K

K

S
x
V

D
|
D

T

G

E

E

A

I

I

I

L

L

H

H

L

L

Y

Y

D

D

D

K

K

G

N

G

I

I

-

E

T

Q

A

T

I

I

P

C

E

M

L

L

D

D

G

G

M

V

F

F

S

A

F

F

A

V

I

L

D

D

-

T

-

A

N

N

N

K

K

K

F

V

I

F

A

L

A

G

R

R

D

D

P

T

I

Y

G

G

I

V

K

R

P

P

L

L

Y

F

Y

K

S

A

-

M

E

T

K

E

D

D

G

G

N

F

F

L

W

A

F

V

A

C

S

S

E

E

L

A

K

K

A

G

I

L

-

V

-

T

-

L

-

K

-

H

-

S

-

A

T

T

Q

P

F

F

C

L

E

K

D

-

V

V

K

E

E

P

F

F

P

L

P

P

G

G

T

H

F

Y

-

E

-

V

-

L

-

D

-

L

-

K

-

P

-

N

-

G

F

K

S

V

S

A

E

S

T

V

G

E

F

M

S

V

T

K

Y

Y

Y

H

N

H

L

C

P

R

D

D

I

V

S

F

P

P

D

G

I

F

D

E

A

-

N

-

V

I

D

E

D

T

I

V

V

K

K

N

E

N

I

L

Q

R

E

I

T

L

V

F

Q

N

A

N

S

A

V

V

V

K

K

K

R

R

L

L

M

M

A

T

D

D

V

R

P

R

L

I

G

G

A

C

F

L

L

L

S

S

G

G

L

L

D

D

S

S

I

L

I

V

A

A

I

T

A

L

K

L

K

K

H

Q

K

L

S

K

E

E

-

A

-

Q

-

V

-

Q

-

Y

-

P

L

L

H

Q

T

T

F

F

S

A

V

I

G

G

V

M

E

E

G

D

S

S

K

P

D

D

I

L

N

L

A

A

R

R

L

K

V

V

S

A

D

D

Y

H

L

I

G

G

T

S

I

E

H

H

H

Y

E

E

Y

V

L

L

I

F

T

N

P

S

K

E

E

E

A

G

I

I

A

Q

K

A

L

L

P

D

E

E

I

V

I

I

Y

F

A

S

L

L

E

E

S

T

F

Y

D

D

Q

I

D
x
T

L

T

V

V

R
|
R

S

A

A

S

I

V

P

G

C

M

Y

Y

F

L

T

I

S

S

R

K

L

Y

A

I

A

R

Q

K

Y

N

V

T

K

D

-

S

-

V

V

V

I

I

L

F

T

S

G

G

E

E

G

G

A

S

D

D

E

E

L

L

F

T

A

Q

G

G

Y

Y

T

I

Y

Y

Y

F

K

H

D

K

I

A

T

P

S

S

D

P

A

E

I

K

L

A

H

E

T

E

E

E

L

S

R

E

R

R

S

L

V

L

S

R

S

E

L

L

H

Y

N

L

I

F

N

D

L

V

Q

L

R

R

V

A

D

D

R

R

L

T

T

T

M

A

A

A

H

H

S

G

I

L

E

E

G

L

R

R

V

V

P

P

F

F

L

L

D

D

L

H

K

R

M

F

I

S

E

S

L

Y

G

Y

Q

L

K

S

I

L

P

P

A

P

H

E

L

M

K

R

L

I

R

P

G

K

N

N

P

-

P

G

V

I

E

E

K

K

W

H

I

L

L

L

R

R

K

E

A

T

F

F

E

E

D

D

-

S

-

N

I

L

L

I

P

P

D

K

E

E

I

I

V

L

W

W

R

R

K

-

K

P

E

S

Q

W

F

F

D

K

E

I

G

L

S

Q

G

E

T

Y

V

V

D

E

L

H

L

Q

V

V

D

D

T

-

L

-

K

-

D

D

I

A

M

M

S

M

E

A

E

N

Q

A

A

A

Q

Q

T

K

Y

F

Q

P

K

F

Q

N

H

T

S

P

H

-

I

-

N

-

L

-

R

K

S

T

A

K

E

E

E

G

C

Y

Y

Y

H

R

Q

Q

I

V

F

F

active site: W4 (≠ A5), L49 (= L48), N74 (= N71), G75 (= G72), T324 (≠ D300), R327 (= R303)
binding 5-oxo-l-norleucine: C1 (= C2), R48 (= R47), V51 (≠ I50), V52 (≠ M51), Y73 (≠ G70), N74 (= N71), G75 (= G72), E76 (= E73), V95 (≠ S94), D96 (= D95)

1mbzA Beta-lactam synthetase with trapped intermediate (see paper)
28% identity, 77% coverage: 72:459/501 of query aligns to 70:465/496 of 1mbzA

query
sites
1mbzA

G
|
G

E

E

I

I

Y

Y

N

N

Y

R

P

D

Q

E

L

L

F

L

S

S

D

V

L

L

E

P

S

A

K

G

Y

P

Q

-

F

-

V

A

T

P

K

E

S

G

D

D

T

A

E

E

A

L

I

V

L

L

H

R

L

L

Y

L

D

E

D

R

K

Y

N

D

I

L

T

H

A

A

I

F

P

R

E

L

L

V

D

N

G

G

M

R

F

F

S

A

F

T

A

V

I

V

I

R

D

T

N

G

N

D

K

R

F

V

I

L

A

L

A

A

R

T

D

D

P

H

I

A

G

G

I

S

K

V

P

P

L

L

Y

Y

Y

T

S

C

E

V

K

A

D

P

G

G

N

E

F

V

W

R

F

A

A

S

S

T

E

E

L

A

K

K

A

A

I

L

T

A

Q

A

F

H

-

G

-

F

-

P

-

L

-

A

-

D

-

A

-

R

-

V

-

A

-

G

C

L

E

T

D

G

V

V

K

Y

E

Q

F

V

P

P

P

A

G

G

T

A

F

V

F

M

S

D

S

I

E

D

T

L

G

G

F

S

S

G

T

T

Y

A

Y

V

N

T

L

H

P

R

D

T

I

W

S

T

P

P

D

G

I

L

D

S

A

R

N

R

V

I

-

L

-

P

-

E

D

G

D

E

I

A

V

V

K

A

E

A

I

V

Q

R

E

A

T

A

V

L

Q

E

A

K

S

A

V

V

V

A

K

Q

R

R

L

V

M

T

-

P

A

G

D

D

V

T

P

P

L

L

G

-

A

V

F
x
V

L
|
L

S
|
S

G

G

G
|
G

L

I

D
|
D

S
|
S

S

S

I

G

I

V

A

A

I

C

A

A

K

H

K

R

H

A

K

A

S

G

E

E

L

L

H

D

T

T

F

V

S
|
S

V
x
M

G

G

V

T

E

D

G

T

S

S

K

N

D

E

I

F

N

R

A

E

A

A

R

R

L

A

V

V

S

V

D

D

Y

H

L

L

G

R

T

T

I

R

H

H

H

R

E

E

Y

I

L

T

I

I

T

P

P

T

K

T

E

E

A

L

I

L

A

A

K

Q

L

L

P

P

E

Y

I

A

I

V

Y

W

A

A

L

S

E

E

S

S

F

V

D

D

Q

P

D
|
D

L

I

V

I

R

E

S
x
Y

A

L

I

L

P

P

C
x
L

Y

T

F

A

T

L

S

Y

R

R

L

A

A

L

A

D

Q

G

Y

P

V

E

K

R

V

R

I

I

L

L

T

T

G
|
G

E
x
Y

G
|
G

A

A

D
|
D

E
x
I

L

P

F

L

A

G

G

G

Y

M

T

H

Y

R

Y

E

K

D

D

R

I

L

T

P

S

A

D

-

A

-

I

-

L

L

H

D

T

T

E

V

L

L

R

A

R

H

S
x
D

V

M

S

A

S

T

L

F

H

D

N

G

I

L

N

N

L
x
E

Q

M

R

S

V

P

D

V

R

L

L

S

T

T

M

L

A

A

H

G

S

H

I

W

E

T

G

T

R

H

V

-

P

P

F

Y

L

W

D

D

L

R

K

E

M

V

I

L

E

D

L

L

G

L

Q

V

K

S

I

L

P

E

A

A

H

G

L

L

K

K

L

R

R

R

G

H

N

G

P

-

V

R

E

D

K
|
K

W

W

I

V

L

L

R

R

K

A

A

A

F

M

E

A

D

D

I

A

L

L

P

P

D

A

E

E

I

T

V

V

W

N

R

R

K

P

K
|
K

E
x
L

Q
x
G

F
x
V

D

H

E

E

G

G

S

S

G

G

T

T

V

T

D

S

L

S

L

F

V

S

D

R

T

L

L

L

K

L

D

D

I

H

-

G

M

V

S

A

E

E

E

D

Q

R

A

V

Q

H

T

E

Y

A

Q

K

K

R

Q

Q

active site: G70 (= G72), D311 (= D300), Y337 (≠ E326), E371 (≠ L363), K432 (= K427)
binding arginine-n-methylcarbonyl phosphoric acid 5'-adenosine ester: V236 (≠ F225), L237 (= L226), S238 (= S227), S243 (= S232), S261 (= S250), M262 (≠ V251), Y315 (≠ S304), L319 (≠ C308), G336 (= G325), Y337 (≠ E326), G338 (= G327), D340 (= D329), I341 (≠ E330), D362 (≠ S354), E371 (≠ L363), K432 (= K427), G434 (≠ Q429), V435 (≠ F430)
binding magnesium ion: D242 (= D231), D340 (= D329)
binding pyrophosphate 2-: S238 (= S227), G240 (= G229), D242 (= D231), S243 (= S232), D340 (= D329), K412 (= K407), K432 (= K427), L433 (≠ E428)

Sites not aligning to the query:

active site: 69

1mc1A Beta-lactam synthetase with product (dgpc), amp and ppi (see paper)
28% identity, 77% coverage: 72:459/501 of query aligns to 66:460/491 of 1mc1A

query
sites
1mc1A

G
|
G

E

E

I

I

Y

Y

N

N

Y

R

P

D

Q

E

L

L

F

L

S

S

D

V

L

L

E

P

S

A

K

G

Y

P

Q

-

F

-

V

A

T

P

K

E

S

G

D

D

T

A

E

E

A

L

I

V

L

L

H

R

L

L

Y

L

D

E

D

R

K

Y

N

D

I

L

T

H

A

A

I

F

P

R

E

L

L

V

D

N

G

G

M

R

F

F

S

A

F

T

A

V

I

V

I

R

D

T

N

G

N

D

K

R

F

V

I

L

A

L

A

A

R

T

D

D

P

H

I

A

G

G

I

S

K

V

P

P

L

L

Y

Y

Y

T

S

C

E

V

K

A

D

P

G

G

N

E

F

V

W

R

F

A

A

S

S

T

E

E

L

A

K

K

A

A

I

L

T

A

Q

A

F

G

-

F

-

P

-

L

-

A

-

D

-

A

-

R

-

V

-

A

-

G

C

L

E

T

D

G

V

V

K

Y

E

Q

F

V

P

P

P

A

G

G

T

A

F

V

F

M

S

D

S

I

E

D

T

L

G

G

F

S

S

G

T

T

Y

A

Y

V

N

T

L

H

P

R

D

T

I

W

S

T

P

P

D

G

I

L

D

S

A

R

N

R

V

I

-

L

-

P

-

E

D

G

D

E

I

A

V

V

K

A

E

A

I

V

Q

R

E

A

T

A

V

L

Q

E

A

K

S

A

V

V

V

A

K

Q

R

R

L

V

M

T

-

P

A

G

D

D

V

T

P

P

L

L

G

-

A

V

F
x
V

L

L

S
|
S

G

G

G
|
G

L

I

D
|
D

S
|
S

S

S

I

G

I

V

A

A

I

C

A

A

K

H

K

R

H

A

K

A

S

G

E

E

L

L

H

D

T

T

F

V

S
|
S

V
x
M

G

G

V

T

E

D

G

T

S

S

K

N

D

E

I

F

N

R

A

E

A

A

R

R

L

A

V

V

S

V

D

D

Y

H

L

L

G

R

T

T

I

R

H

H

H

R

E

E

Y

I

L

T

I

I

T

P

P

T

K

T

E

E

A

L

I

L

A

A

K

Q

L

L

P

P

E

Y

I

A

I

V

Y

W

A

A

L

S

E

E

S

S

F

V

D

D

Q

P

D
|
D

L

I

V

I

R

E

S
x
Y

A

L

I

L

P

P

C

L

Y

T

F

A

T

L

S

Y

R

R

L

A

A

L

A

D

Q

G

Y

P

V

E

K

R

V

R

I

I

L

L

T

T

G
|
G

E
x
Y

G
|
G

A

A

D
|
D

E
x
I

L

P

F

L

A

G

G

G

Y

M

T

H

Y

R

Y

E

K

D

D

R

I

L

T

P

S

A

D

-

A

-

I

-

L

L

H

D

T

T

E

V

L

L

R

A

R

H

S
x
D

V

M

S

A

S

T

L

F

H

D

N

G

I

L

N

N

L
x
E

Q

M

R

S

V

P

D

V

R

L

L

S

T

T

M

L

A

A

H

G

S

H

I

W

E

T

G

T

R

H

V

-

P

P

F

Y

L

W

D

D

L

R

K

E

M

V

I

L

E

D

L

L

G

L

Q

V

K

S

I

L

P

E

A

A

H

G

L

L

K

K

L

R

R

R

G

H

N

G

P

-

V

R

E

D

K
|
K

W

W

I

V

L

L

R

R

K

A

A

A

F

M

E

A

D

D

I

A

L

L

P

P

D

A

E

E

I

T

V

V

W

N

R

R

K

P

K
|
K

E
x
L

Q

G

F
x
V

D

H

E

E

G

G

S

S

G

G

T

T

V

T

D

S

L

S

L

F

V

S

D

R

T

L

L

L

K

L

D

D

-

H

I

G

M

V

S

A

E

E

E

D

Q

R

A

V

Q

H

T

E

Y

A

Q

K

K

R

Q

Q

active site: G66 (= G72), D306 (= D300), Y332 (≠ E326), E366 (≠ L363), K427 (= K427)
binding adenosine monophosphate: V231 (≠ F225), S233 (= S227), S238 (= S232), S256 (= S250), M257 (≠ V251), G331 (= G325), K427 (= K427), V430 (≠ F430)
binding magnesium ion: D237 (= D231), D335 (= D329)
binding deoxyguanidinoproclavaminic acid: Y310 (≠ S304), Y332 (≠ E326), G333 (= G327), I336 (≠ E330), D357 (≠ S354), E366 (≠ L363), K427 (= K427)
binding pyrophosphate 2-: S233 (= S227), G235 (= G229), D237 (= D231), S238 (= S232), D335 (= D329), K407 (= K407), K427 (= K427), L428 (≠ E428)

Sites not aligning to the query:

active site: 65

1mb9A Beta-lactam synthetase complexed with atp (see paper)
29% identity, 72% coverage: 72:430/501 of query aligns to 71:434/485 of 1mb9A

query
sites
1mb9A

G
|
G

E

E

I

I

Y

Y

N

N

Y

R

P

D

Q

E

L

L

F

L

S

S

D

V

L

L

E

P

S

A

K

G

Y

P

Q

-

F

-

V

A

T

P

K

E

S

G

D

D

T

A

E

E

A

L

I

V

L

L

H

R

L

L

Y

L

D

E

D

R

K

Y

N

D

I

L

T

H

A

A

I

F

P

R

E

L

L

V

D

N

G

G

M

R

F

F

S

A

F

T

A

V

I

V

I

R

D

T

N

G

N

D

K

R

F

V

I

L

A

L

A

A

R

T

D

D

P

H

I

A

G

G

I

S

K

V

P

P

L

L

Y

Y

Y

T

S

C

E

V

K

A

D

P

G

G

N

E

F

V

W

R

F

A

A

S

S

T

E

E

L

A

K

K

A

A

I

L

T

A

Q

A

F

F

-

P

-

L

-

A

-

D

-

A

-

R

-

V

-

A

-

G

C

L

E

T

D

G

V

V

K

Y

E

Q

F

V

P

P

P

A

G

G

T

A

F

V

F

M

S

D

S

I

E

D

T

L

G

G

F

S

S

G

T

T

Y

A

Y

V

N

T

L

H

P

R

D

T

I

W

S

T

P

P

D

G

I

L

D

S

A

R

N

R

V

I

-

L

-

P

-

E

D

G

D

E

I

A

V

V

K

A

E

A

I

V

Q

R

E

A

T

A

V

L

Q

E

A

K

S

A

V

V

V

A

K

Q

R

R

L

V

M

T

-

P

A

G

D

D

V

T

P

P

L

L

G

-

A

V

F
x
V

L
|
L

S
|
S

G

G

G
|
G

L

I

D
|
D

S
|
S

S

S

I

G

I

V

A

A

I

C

A

A

K

H

K

R

H

A

K

A

S

G

E

E

L

L

H

D

T

T

F

V

S
|
S

V
x
M

G

G

V

T

E

D

G

T

S

S

K

N

D

E

I

F

N

R

A

E

A

A

R

R

L

A

V

V

S

V

D

D

Y

H

L

L

G

R

T

T

I

R

H

H

H

R

E

E

Y

I

L

T

I

I

T

P

P

T

K

T

E

E

A

L

I

L

A

A

K

Q

L

L

P

P

E

Y

I

A

I

V

Y

W

A

A

L

S

E

E

S

S

F

V

D

D

Q

P

D
|
D

L

I

V

I

R

E

S
x
Y

A

L

I

L

P

P

C
x
L

Y

T

F

A

T

L

S

Y

R

R

L

A

A

L

A

D

Q

G

Y

P

V

E

K

R

V

R

I

I

L

L

T

T

G
|
G

E
x
Y

G

G

A

A

D
|
D

E

I

L

P

F

L

A

G

G

G

Y

M

T

H

Y

R

Y

E

K

D

D

R

I

L

T

P

S

A

D

-

A

-

I

-

L

L

H

D

T

T

E

V

L

L

R

A

R

H

S

D

V

M

S

A

S

T

L

F

H

D

N

G

I

L

N

N

L
x
E

Q

M

R

S

V

P

D

V

R

L

L

S

T

T

M

L

A

A

H

G

S

H

I

W

E

T

G

T

R

H

V

-

P

P

F

Y

L

W

D

D

L

R

K

E

M

V

I

L

E

D

L

L

G

L

Q

V

K

S

I

L

P

E

A

A

H

G

L

L

K

K

L

R

R

R

G

H

N

G

P

-

V

R

E

D

K
|
K

W

W

I

V

L

L

R

R

K

A

A

A

F

M

E

A

D

D

I

A

L

L

P

P

D

A

E

E

I

T

V

V

W

N

R

R

K

P

K
|
K

E

S

Q

S

F

F

active site: G71 (= G72), D310 (= D300), Y336 (≠ E326), E370 (≠ L363), K431 (= K427)
binding adenosine monophosphate: V235 (≠ F225), L236 (= L226), S242 (= S232), S260 (= S250), M261 (≠ V251), Y314 (≠ S304), L318 (≠ C308), G335 (= G325), Y336 (≠ E326)
binding adenosine-5'-triphosphate: V235 (≠ F225), L236 (= L226), S237 (= S227), G239 (= G229), D241 (= D231), S242 (= S232), S260 (= S250), M261 (≠ V251), L318 (≠ C308), G335 (= G325), D339 (= D329), K411 (= K407), K431 (= K427)
binding magnesium ion: D241 (= D231), D339 (= D329)
binding pyrophosphate 2-: S237 (= S227), G239 (= G229), D241 (= D231), S242 (= S232), D339 (= D329), K411 (= K407), K431 (= K427)

Sites not aligning to the query:

active site: 70

1jgtB Crystal structure of beta-lactam synthetase (see paper)
28% identity, 71% coverage: 72:427/501 of query aligns to 74:440/500 of 1jgtB

query
sites
1jgtB

G
|
G

E

E

I

I

Y

Y

N

N

Y

R

P

D

Q

E

L

L

F

L

S

S

D

V

L

L

E

P

S

A

K

G

Y

P

Q

-

F

-

V

A

T

P

K

E

S

G

D

D

T

A

E

E

A

L

I

V

L

L

H

R

L

L

Y

L

D

E

D

R

K

Y

N

D

I

L

T

H

A

A

I

F

P

R

E

L

L

V

D

N

G

G

M

R

F

F

S

A

F

T

A

V

I

V

I

R

D

T

N

G

N

D

K

R

F

V

I

L

A

L

A

A

R

T

D

D

P

H

I

A

G

G

I

S

K

V

P

P

L

L

Y

Y

Y

T

S

C

E

V

K

A

D

P

G

G

N

E

F

V

W

R

F

A

A

S

S

T

E

E

L

A

K

K

A

A

I

L

T

A

Q

A

F

H

C

R

E

D

-

P

-

K

-

G

-

F

-

P

-

L

-

A

-

D

-

A

-

R

-

V

-

A

-

G

-

L

-

T

D

G

V

V

K

Y

E

Q

F

V

P

P

P

A

G

G

T

A

F

V

F

M

S

D

S

I

E

D

T

L

G

G

F

S

S

G

T

T

Y

A

Y

V

N

T

L

H

P

R

D

T

I

W

S

T

P

P

D

G

I

L

D

S

A

R

N

R

V

I

-

L

-

P

-

E

D

G

D

E

I

A

V

V

K

A

E

A

I

V

Q

R

E

A

T

A

V

L

Q

E

A

K

S

A

V

V

V

A

K

Q

R

R

L

V

M

T

-

P

A

G

D

D

V

T

P

P

L

L

G

-

A

V

F
x
V

L
|
L

S
|
S

G

G

G
|
G

L
x
I

D
|
D

S
|
S

S

S

I

G

I

V

A

A

I

C

A

A

K

H

K

R

H

A

K

A

S

G

E

E

L

L

H

D

T

T

F

V

S
|
S

V
x
M

G

G

V

T

E

D

G

T

S

S

K

N

D

E

I

F

N

R

A

E

A

A

R

R

L

A

V

V

S

V

D

D

Y

H

L

L

G

R

T

T

I

R

H

H

H

R

E

E

Y

I

L

T

I

I

T

P

P

T

K

T

E

E

A

L

I

L

A

A

K

Q

L

L

P

P

E

Y

I

A

I

V

Y

W

A

A

L

S

E

E

S

S

F

V

D

D

Q

P

D
|
D

L

I

V

I

R

E

S
x
Y

A

L

I

L

P

P

C
x
L

Y

T

F

A

T

L

S

Y

R

R

L

A

A

L

A

D

Q

G

Y

P

V

E

K

R

V

R

I

I

L

L

T

T

G
|
G

E
x
Y

G
|
G

A

A

D
|
D

E
x
I

L

P

F

L

A

G

G

G

Y
x
M

T

H

Y

R

Y

E

K

D

D

R

I

L

T

P

S

A

D

-

A

-

I

-

L

L

H

D

T

T

E

V

L

L

R

A

R

H

S
x
D

V

M

S

A

S

T

L

F

H

D

N

G

I

L

N

N

L
x
E

Q

M

R

S

V

P

D

V

R

L

L

S

T

T

M

L

A

A

H

G

S

H

I

W

E

T

G

T

R

H

V

-

P

P

F

Y

L

W

D

D

L

R

K

E

M

V

I

L

E

D

L

L

G

L

Q

V

K

S

I

L

P

E

A

A

H

G

L

L

K

K

L

R

R

R

G

H

N

G

P

-

V

R

E

D

K
|
K

W

W

I

V

L

L

R

R

K

A

A

A

F

M

E

A

D

D

I

A

L

L

P

P

D

A

E

E

I

T

V

V

W

N

R

R

K

P

K
|
K

active site: G74 (= G72), D319 (= D300), Y345 (≠ E326), E379 (≠ L363), K440 (= K427)
binding diphosphomethylphosphonic acid adenosyl ester: V244 (≠ F225), L245 (= L226), S246 (= S227), G248 (= G229), I249 (≠ L230), D250 (= D231), S251 (= S232), S269 (= S250), M270 (≠ V251), L327 (≠ C308), G344 (= G325), Y345 (≠ E326), D348 (= D329), K420 (= K407), K440 (= K427)
binding n2-(carboxyethyl)-l-arginine: Y323 (≠ S304), Y345 (≠ E326), G346 (= G327), D348 (= D329), I349 (≠ E330), M354 (≠ Y335), D370 (≠ S354), E379 (≠ L363)
binding magnesium ion: D250 (= D231), D348 (= D329)

Sites not aligning to the query:

active site: 73

1q19A Carbapenam synthetase (see paper)
29% identity, 47% coverage: 222:455/501 of query aligns to 240:470/500 of 1q19A

query
sites
1q19A

L

V

G

G

A

I

F
x
P

L
|
L

S
|
S

G

G

L

L

D
|
D

S
|
S

S

S

I

L

I

V

A

T

A

A

I

L

A

A

K

S

K

R

H

H

K

F

S

K

E

K

L

L

H

N

T

T

F

Y

S
|
S

V
x
I

G

G

V

T

E

E

G

L

S

S

K

N

D

E

I

F

N

E

A

F

A

S

R

Q

L

Q

V

V

S

A

D

D

Y

A

L

L

G

G

T

T

I

H

H

H

H

Q

E

M

Y

K

L

I

I

L

T

S

P

E

K

T

E

E

A

V

I

I

A

N

K

G

L

I

P

I

E

E

I

S

I

I

Y

Y

A

Y

L

N

E

E

S

I

F

F

D

D

Q

G

D
x
L

L

S

V

A

R
x
E

S

I

A

Q

I

S

P

G

C

L

Y

F

F

N

T

V

S

Y

R

R

L

Q

A

A

A

Q

Q

G

Y

Q

V

V

K

S

V

C

I

M

L

L

T
|
T

G
|
G

E
x
Y

G
|
G

A

S

D
|
D

E
x
L

L

L

F

F

A

G

G

G

-

I

-

L

-

K

-

P

-

G

-

A

-

Q

Y

Y

T

D

Y

N

Y

P

K

N

D

Q

I

L

T

L

S

A

D

E

A

Q

I

V

L

Y

H
x
R

T

T

E

R

L

W

R

T

R

G

S
x
E

V

F

S

A

S

T

L

-

H

H

N

G

I

A

N

S

L

-

Q

-

R

-

V

-

D

-

R

-

L

-

T

-

M

-

A

C

H

Y

S

G

I

I

E

D

G

I

R

R

V

H

P

P

F

F

L

W

D

S

L

H

K

S

M

L

I

I

E

S

L

L

G

C

Q

H

K

A

I

L

P

H

A

P

H

D

L

Y

K

K

L

I

R

F

G

D

N

N

P

E

P

V

V

-

E

-

K

K

W

N

I

I

L

L

R

R

K

E

A

Y

F

A

E

D

D

S

-

L

-

Q

I

L

L

L

P

P

D

K

E

D

I

I

V

V

W

W

R

R

K

K

K
|
K

E
x
I

Q
x
G

F
x
I

D

H

E

E

G

G

S

S

G

S

T

V

V

N

D

Q

L

A

L

F

V

A

D

N

T

V

L

L

K

G

D

S

I

T

M

V

S

D

E

N

E

Y

Q

Q

A

T

Q

K

T

S

active site: L318 (≠ D300), E321 (≠ R303), Y344 (≠ E326), E379 (≠ S354), K442 (= K427)
binding diphosphomethylphosphonic acid adenosyl ester: P243 (≠ F225), L244 (= L226), S245 (= S227), D249 (= D231), S250 (= S232), S268 (= S250), I269 (≠ V251), T342 (= T324), G343 (= G325), D347 (= D329), K442 (= K427), I443 (≠ E428), G444 (≠ Q429), I445 (≠ F430)
binding (2s,5s)-5-carboxymethylproline: Y344 (≠ E326), G345 (= G327), L348 (≠ E330), R373 (≠ H348), E379 (≠ S354)

Sites not aligning to the query:

active site: 56

Q9XB61 Carbapenam-3-carboxylate synthase; Carbapenam-3-carboxylate ligase; EC 6.3.3.6 from Pectobacterium carotovorum subsp. carotovorum (Erwinia carotovora subsp. carotovora) (see 3 papers)
29% identity, 47% coverage: 222:455/501 of query aligns to 241:471/503 of Q9XB61

query
sites
Q9XB61

L

V

G

G

A

I

F
x
P

L
|
L

S
|
S

G
|
G

L
|
L

D
|
D

S
|
S

S

S

I

L

I

V

A

T

A

A

I

L

A

A

K

S

K

R

H

H

K

F

S

K

E

K

L

L

H

N

T

T

F

Y

S

S

V
x
I

G

G

V

T

E

E

G

L

S

S

K

N

D

E

I

F

N

E

A

F

A

S

R

Q

L

Q

V

V

S

A

D

D

Y

A

L

L

G

G

T

T

I

H

H

H

H

Q

E

M

Y

K

L

I

I

L

T

S

P

E

K

T

E

E

A

V

I

I

A

N

K

G

L

I

P

I

E

E

I

S

I

I

Y

Y

A

Y

L

N

E

E

S

I

F

F

D

D

Q

G

D

L

L

S

V

A

R

E

S

I

A

Q

I

S

P

G

C

L

Y

F

F

N

T

V

S

Y

R

R

L

Q

A

A

A

Q

Q

G

Y

Q

V

V

K

S

V

C

I

M

L

L

T

T

G
|
G

E
x
Y

G
|
G

A
x
S

D
|
D

E

L

L

L

F

F

A

G

G

G

-

I

-

L

-

K

-

P

-

G

-

A

-

Q

Y

Y

T

D

Y

N

Y

P

K

N

D

Q

I

L

T

L

S

A

D

E

A
x
Q

I

V

L

Y

H
x
R

T

T

E

R

L

W

R

T

R

G

S
x
E

V

F

S

A

S

T

L

-

H

H

N

G

I

A

N

S

L

-

Q

-

R

-

V

-

D

-

R

-

L

-

T

-

M

-

A

C

H

Y

S

G

I

I

E

D

G

I

R

R

V

H

P

P

F

F

L

W

D

S

L

H

K

S

M

L

I

I

E

S

L

L

G

C

Q

H

K

A

I

L

P

H

A

P

H

D

L

Y

K

K

L

I

R

F

G

D

N

N

P

E

P

V

V

-

E

-

K
|
K

W

N

I

I

L

L

R

R

K

E

A

Y

F

A

E

D

D

S

-

L

-

Q

I

L

L

L

P

P

D

K

E

D

I

I

V

V

W

W

R

R

K

K

K
|
K

E
x
I

Q
x
G

F
x
I

D

H

E

E

G

G

S

S

G

S

T

V

V

N

D

Q

L

A

L

F

V

A

D

N

T

V

L

L

K

G

D

S

I

T

M

V

S

D

E

N

E

Y

Q

Q

A

T

Q

K

T

S

244:251 (vs. 225:232, 88% identical) binding ATP
I270 (≠ V251) binding ATP
GYGSD 344:348 (≠ GEGAD 325:329) binding ATP
Y345 (≠ E326) mutation to A: Loss of activity.; mutation to F: Reduces catalytic efficiency.
G346 (= G327) binding substrate
Q371 (≠ A345) binding substrate
R374 (≠ H348) binding substrate
E380 (≠ S354) mutation to A: Loss of activity.; mutation to D: Reduces catalytic efficiency.; mutation to Q: Reduces catalytic efficiency.
K421 (= K407) binding ATP
K443 (= K427) mutation K->A,M: Loss of activity.
IGI 444:446 (≠ EQF 428:430) binding ATP

6lbpA Structure of the glutamine phosphoribosylpyrophosphate amidotransferase from arabidopsis thaliana (see paper)
29% identity, 31% coverage: 2:158/501 of query aligns to 1:199/460 of 6lbpA

query
sites
6lbpA

C
|
C

G

G

I

V

A

V

G

G

I

I

W

Y

G

G

K

D

T

S

S

E

Q

A

S

S

N

R

I

L

E

C

A

Y

M

L

-

A

M

L

E

H

S

A

I

L

V

Q

H

H

R

R

G
|
G

P

Q

D

E

A

G

N

A

G

G

I

I

F

V

V

T

V

V

P

S

D

K

-

D

-

K

-

V

-

L

-

Q

-

T

-

I

-

T

G

G

S

V

G

G

I

L

-

V

-

S

-

E

-

V

-

F

-

S

-

E

-

S

-

K

-

L

-

D

-

Q

-

L

-

P

-

G

-

D

-

I

-

A

L

I

G

G

H

H

Q

V

R

R

L

Y

S

S

I

T

M

A

-

G

-

S

D

S

V

M

E

L

G

K

G

N

D

V

Q

Q

P

P

I

F

-

V

-

A

-

G

Y

Y

G

R

D

F

G

G

K

S

K

V

A

G

I

V

I

A

G

H

N
|
N

G
|
G

E

N

I

L

Y

V

N

N

Y

Y

P

T

Q

K

L

L

F

R

S

A

D

D

L

L

E

E

S

E

K

N

Y

G

Q

S

-

I

F

F

V

N

T

T

K

S

S

S

D

D

T

T

E

E

A

V

I

V

L

L

H

H

L

L

-

I

-

A

-

I

-

S

-

K

-

A

-

R

-

P

Y

F

D

F

D

M

K

R

N

I

I

V

T

D

A

A

I

C

P

E

E

K

L

L

D

Q

G

G

M

A

F

Y

S

S

F

M

A

V

I

F

I

V

D

T

N

E

N

D

K

K

F

L

I

V

A

A

A

V

R

R

D

D

P

P

I

H

G

G

I

F

K

R

P

P

L

L

Y

V

Y

M

S

G

E

R

K

R

-

S

D

N

G

G

N

A

F

V

W

V

F

F

A

A

S

S

E

E

L

T

K

C

A

A

I

L

active site: C1 (= C2), G27 (= G27), N102 (= N71), G103 (= G72)

Sites not aligning to the query:

active site: 243, 301, 306, 316, 424
binding iron/sulfur cluster: 237, 239, 383, 385, 434, 436, 437

Q9STG9 Amidophosphoribosyltransferase 2, chloroplastic; AtATase2; AtPURF2; PRPP2; Glutamine phosphoribosylpyrophosphate amidotransferase 2; AtGPRAT2; Protein CHLOROPLAST IMPORT APPARATUS 1; Protein DIFFERENTIAL DEVELOPMENT OF VASCULAR ASSOCIATED CELLS; EC 2.4.2.14 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
29% identity, 31% coverage: 2:158/501 of query aligns to 87:285/561 of Q9STG9

query
sites
Q9STG9

C

C

G

G

I

V

A

V

G

G

I

I

W

Y

G

G

K

D

T

S

S

E

Q

A

S

S

N

R

I

L

E

C

A

Y

M

L

-

A

M

L

E

H

S

A

I

L

V

Q

H

H

R

R

G

G

P

Q

D

E

A

G

N

A

G

G

I

I

F

V

V

T

V

V

P

S

D

K

-

D

-

K

-

V

-

L

-

Q

-

T

-

I

-

T

G

G

S

V

G

G

I

L

-

V

-

S

-

E

-

V

-

F

-

S

-

E

-

S

-

K

-

L

-

D

-

Q

-

L

-

P

-

G

-

D

-

I

-

A

L

I

G

G

H

H

Q

V

R

R

L

Y

S

S

I

T

M

A

-

G

-

S

D

S

V

M

E

L

G

K

G

N

D

V

Q

Q

P

P

I

F

-

V

-

A

-

G

Y

Y

G

R

D

F

G

G

K

S

K

V

A

G

I

V

I

A

G
x
H

N

N

G

G

E

N

I

L

Y

V

N

N

Y

Y

P

T

Q

K

L

L

F

R

S

A

D

D

L

L

E

E

S

E

K

N

Y

G

Q

S

-

I

F

F

V

N

T

T

K

S

S

S

D

D

T

T

E

E

A

V

I

V

L

L

H

H

L

L

-

I

-

A

-

I

-

S

-

K

-

A

-

R

-

P

Y

F

D

F

D

M

K

R

N

I

I

V

T

D

A

A

I

C

P

E

E

K

L

L

D

Q

G

G

M

A

F

Y

S

S

F

M

A

V

I

F

I

V

D

T

N

E

N

D

K

K

F

L

I

V

A

A

A

V

R

R

D

D

P

P

I

H

G

G

I

F

K
x
R

P
|
P

L

L

Y

V

Y

M

S

G

E

R

K

R

-

S

D

N

G

G

N

A

F

V

W

V

F

F

A

A

S

S

E

E

L

T

K

C

A

A

I

L

H187 (≠ G70) mutation to T: In cia1-2; small plants with white leaves showing an irregular mosaic of green sectors.
R264 (≠ K138) mutation to K: Strong resistance to the bleaching herbicides DAS073 and DAS734.
P265 (= P139) mutation to S: Low resistance to the bleaching herbicides DAS073 and DAS734; when associated with F-494.

Sites not aligning to the query:

371 G→S: Low resistance to the bleaching herbicides DAS073 and DAS734.
476 P→S: Resistance to the bleaching herbicides DAS073 and DAS734.
494 Y→F: Low resistance to the bleaching herbicides DAS073 and DAS734; when associated with S-265.

P00497 Amidophosphoribosyltransferase; ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase; EC 2.4.2.14 from Bacillus subtilis (strain 168) (see 5 papers)
27% identity, 48% coverage: 2:239/501 of query aligns to 12:301/476 of P00497

query
sites
P00497

C
|
C

G

G

I

V

A

F

G

G

I

I

W

W

G

G

K

H

T

E

S

E

Q

A

S

P

N

Q

I

I

E

T

A

Y

M

Y

-

G

M

L

E

H

S

S

I

L

V

Q

H

H

R

R

G

G

P

Q

D

E

A

G

N

A

G

G

I

I

F

V

V

A

V

T

P

D

-

G

-

E

-

K

-

L

-

T

-

A

-

H

-

K

-

G

-

Q

-

G

-

L

-

I

-

T

-

E

-

V

-

F

-

Q

-

N

-

G

-

E

-

L

-

S

-

K

-

V

D

K

G

G

S

K

G

G

I

A

L

I

G

G

H

H

Q

V

R

R

L

Y

S

A

I

T

M

A

D

G

V

G

E

G

G

G

G

Y

D

E

-

N

-

V

Q

Q

P

P

I

L

Y

L

-

F

-

R

-

S

-

Q

G

N

D

N

G

G

K

S

K

L

A

A

I

L

I

A

G

H

N

N

G

G

E

N

I

L

Y

V

N

N

Y

A

P

T

Q

Q

L

L

F

K

S

Q

D

Q

L

L

E

E

S

N

K

Q

Y

G

Q

S

-

I

F

F

V

Q

T

T

K

S

S

S

D

D

T

T

E

E

A

V

I

L

L

A

H

H

L

L

-

I

-

K

-

R

-

S

-

G

-

H

-

F

-

T

Y

L

D

K

D

D

K

Q

N

I

I

K

T

N

A

S

I

L

P

S

E

M

L

L

D

K

G

G

M

A

F

Y

S

A

F

F

A

L

I

I

I

M

D

T

N

E

N

T

K

E

F

M

I

I

A

V

A

A

R

L

D

D

P

P

I

N

G

G

I

L

K

R

P

P

L

L

Y

S

Y

I

S

G

E

M

K

M

D

G

G

D

N

A

F

Y

W

V

F

V

A

A

S

S

E

E

L

T

K

C

A

A

I

F

T

D

Q

V

F

V

C

G

E

A

D

T

-

Y

V

L

K

R

E

E

F

V

P

E

P

P

G

G

T

E

F

M

-

L

-

I

-

N

-

D

-

E

-

G

-

M

-

K

-

S

-

E

-

R

F

F

S

S

S

M

E

N

T

I

G

N

F

R

S

S

-

I

-
x
C

-

S

-

M

-

E

-

Y

T

I

Y

Y

Y

F

N

S

L

R

P

P

D

D

I

-

S

-

P

S

D

N

I

I

D

D

A

G

-

I

N

N

V

V

D

H

D

S

I

A

V

R

K

K

E

N

I

L

Q

G

E

K

T

M

V

L

-

A

Q

Q

A

E

S

S

V

A

V

V

K

E

R

-

L

-

M

-

A

A

D

D

V

V

P

-

L

-

G

-

A

-

F

-

L

V

S

T

G

G

G

V

L

P

D

D

S
|
S

S

S

I

I

I

S

A

A

I

I

A

G

C12 (= C2) active site, Nucleophile; mutation to F: Loss of enzyme activity and N-terminal processing.
C247 (vs. gap) binding [4Fe-4S] cluster
S294 (= S232) binding Mg(2+)

Sites not aligning to the query:

1:11 modified: propeptide
356 binding Mg(2+)
357 binding Mg(2+)
393 binding [4Fe-4S] cluster
394 F→V: Partial loss of activity.
442 D→S: Partial loss of activity.
448 binding [4Fe-4S] cluster; C→S: Loss of activity.
451 binding [4Fe-4S] cluster; C→S: Loss of activity.
452 F→C: Lethal.

1gph1 Structure of the allosteric regulatory enzyme of purine biosynthesis (see paper)
27% identity, 48% coverage: 2:239/501 of query aligns to 1:290/465 of 1gph1

query
sites
1gph1

C

C

G

G

I

V

A

F

G

G

I

I

W

W

G

G

K

H

T

E

S

E

Q

A

S

P

N

Q

I

I

E

T

A

Y

M

Y

-

G

M

L

E

H

S

S

I

L

V

Q

H

H

R

R

G

G

P

Q

D

E

A

G

N

A

G

G

I

I

F

V

V

A

V

T

P

D

-

G

-

E

-

K

-

L

-

T

-

A

-

H

-

K

-

G

-

Q

-

G

-

L

-

I

-

T

-

E

-

V

-

F

-

Q

-

N

-

G

-

E

-

L

-

S

-

K

-

V

D

K

G

G

S

K

G

G

I

A

L

I

G

G

H

H

Q

V

R

R

L

Y

S

A

I

T

M

A

D

G

V

G

E

G

G

G

G

Y

D

E

-

N

-

V

Q

Q

P

P

I

L

Y

L

-

F

-

R

-

S

-

Q

G

N

D

N

G

G

K

S

K

L

A

A

I

L

I

A

G

H

N

N

G

G

E

N

I

L

Y

V

N

N

Y

A

P

T

Q

Q

L

L

F

K

S

Q

D

Q

L

L

E

E

S

N

K

Q

Y

G

Q

S

-

I

F

F

V

Q

T

T

K

S

S

S

D

D

T

T

E

E

A

V

I

L

L

A

H

H

L

L

-

I

-

K

-

R

-

S

-

G

-

H

-

F

-

T

Y

L

D

K

D

D

K

Q

N

I

I

K

T

N

A

S

I

L

P

S

E

M

L

L

D

K

G

G

M

A

F

Y

S

A

F

F

A

L

I

I

I

M

D

T

N

E

N

T

K

E

F

M

I

I

A

V

A

A

R

L

D

D

P

P

I

N

G

G

I

L

K

R

P

P

L

L

Y

S

Y

I

S

G

E

M

K

M

D

G

G

D

N

A

F

Y

W

V

F

V

A

A

S

S

E

E

L

T

K

C

A

A

I

F

T

D

Q

V

F

V

C

G

E

A

D

T

-

Y

V

L

K

R

E

E

F

V

P

E

P

P

G

G

T

E

F

M

-

L

-

I

-

N

-

D

-

E

-

G

-

M

-

K

-

S

-

E

-

R

F

F

S

S

S

M

E

N

T

I

G

N

F

R

S

S

-

I

-
x
C

-
x
S

-

M

-

E

-

Y

T

I

Y
|
Y

Y

F

N
x
S

L
x
R

P
|
P

D

D

I

-

S

-

P

S

D

N

I

I

D

D

A

G

-

I

N

N

V

V

D

H

D

S

I

A

V

R

K

K

E

N

I

L

Q

G

E

K

T

M

V

L

-

A

Q

Q

A

E

S

S

V

A

V

V

K

E

R

-

L

-

M

-

A

A

D

D

V

V

P

-

L

-

G

-

A

-

F

-

L

V

S

T

G

G

G

V

L

P

D
|
D

S
|
S

S

S

I

I

I

S

A

A

I

I

A

G

binding adenosine monophosphate: Y242 (= Y183), Y242 (= Y183), S244 (≠ N185), R245 (≠ L186), P246 (= P187), D282 (= D231), S283 (= S232), S283 (= S232)
binding iron/sulfur cluster: C236 (vs. gap), S237 (vs. gap)

Sites not aligning to the query:

active site: 300, 305, 315, 423
binding adenosine monophosphate: 304, 305, 307, 345, 346, 347, 349, 350, 353, 388
binding iron/sulfur cluster: 382, 384, 388, 437, 440

1ao0A Glutamine phosphoribosylpyrophosphate (prpp) amidotransferase from b. Subtilis complexed with adp and gmp (see paper)
28% identity, 48% coverage: 2:239/501 of query aligns to 1:286/455 of 1ao0A

query
sites
1ao0A

C
|
C

G

G

I

V

A

F

G

G

I

I

W

W

G

G

K

H

T

E

S

E

Q

A

S

P

N

Q

I

I

E

T

A

Y

M

Y

-

G

M

L

E

H

S

S

I

L

V

Q

H
|
H

R

R

G
|
G

P

Q

D

E

A

G

N

A

G

G

I

I

F

-

V

V

V

A

P

T

D

D

G

G

S

E

G

K

I

L

L

T

G

A

H

H

-

K

-

G

-

Q

-

G

-

L

-

I

-

T

-

E

-

V

-

F

Q

Q

R

N

L

G

S

E

I

L

M

S

D

K

V

V

E

K

G

G

G

K

D

G

-

A

-

I

-

G

-

H

-

V

-

R

-

Y

-

A

-

T

-

G

-

Y

-

E

-

N

-

V

Q

Q

P

P

I

L

Y

L

-

F

-

R

-

S

-

Q

G

N

D

N

G

G

K

S

K

L

A

A

I

L

I

A

G

H

N
|
N

G
|
G

E

N

I

L

Y

V

N

N

Y

A

P

T

Q

Q

L

L

F

K

S

Q

D

Q

L

L

E

E

S

N

K

Q

Y

G

Q

S

-

I

F

F

V

Q

T

T

K

S

S

S

D

D

T

T

E

E

A

V

I

L

L

A

H

H

L

L

-

I

-

K

-

R

-

S

-

G

-

H

-

F

-

T

Y

L

D

K

D

D

K

Q

N

I

I

K

T

N

A

S

I

L

P

S

E

M

L

L

D

K

G

G

M

A

F

Y

S

A

F

F

A

L

I

I

I

M

D

T

N

E

N

T

K

E

F

M

I

I

A

V

A

A

R

L

D

D

P

P

I

N

G

G

I

L

K

R

P

P

L

L

Y

S

Y

I

S

G

E

M

K

M

D

G

G

D

N

A

F

Y

W

V

F

V

A

A

S

S

E

E

L

T

K

C

A

A

I

F

T

D

Q

V

F

V

C

G

E

A

D

T

-

Y

V

L

K

R

E

E

F

V

P

E

P

P

G

G

T

E

F

M

-

L

-

I

-

N

-

D

-

E

-

G

-

M

-

K

-

S

-

E

-

R

F

F

S

S

S

M

E

N

T

I

G

N

F

R

S

S

-

I

-
x
C

-
x
S

-
x
M

-

E

-

Y

T

I

Y
|
Y

Y

F

N
x
S

L
x
R

P
|
P

D

D

I

-

S

-

P

S

D

N

I

I

D

D

A

G

-

I

N

N

V

V

D

H

D

S

I

A

V

R

K

K

E

N

I

L

Q

G

E

K

T

M

V

L

-

A

Q

Q

A

E

S

S

V

A

V

V

K

E

R

-

L

-

M

-

A

A

D

D

V

V

P

-

L

-

G

-

A

-

F

-

L

V

S

T

G

G

G

V

L
x
P

D
|
D

S
|
S

S

S

I

I

I

S

A

A

I

I

A

G

active site: C1 (= C2), G27 (= G27), N98 (= N71), G99 (= G72), Y238 (= Y183)
binding guanosine-5'-monophosphate: M234 (vs. gap), Y238 (= Y183), S279 (= S232)
binding adenosine-5'-diphosphate: H25 (= H25), Y238 (= Y183), S240 (≠ N185), R241 (≠ L186), P242 (= P187), P277 (≠ L230), D278 (= D231), S279 (= S232)
binding magnesium ion: S279 (= S232)
binding iron/sulfur cluster: C232 (vs. gap), S233 (vs. gap), M234 (vs. gap)

Sites not aligning to the query:

active site: 296, 301, 311, 419
binding guanosine-5'-monophosphate: 341, 342, 343, 345, 346, 347, 348, 349
binding adenosine-5'-diphosphate: 300, 301, 301, 303
binding magnesium ion: 341, 342
binding iron/sulfur cluster: 378, 380, 433, 436

Query Sequence

>WP_009543906.1 NCBI__GCF_000017845.1:WP_009543906.1
MCGIAGIWGKTSQSNIEAMMESIVHRGPDANGIFVVPDGSGILGHQRLSIMDVEGGDQPI
YGDGKKAIIGNGEIYNYPQLFSDLESKYQFVTKSDTEAILHLYDDKNITAIPELDGMFSF
AIIDNNKFIAARDPIGIKPLYYSEKDGNFWFASELKAITQFCEDVKEFPPGTFFSSETGF
STYYNLPDISPDIDANVDDIVKEIQETVQASVVKRLMADVPLGAFLSGGLDSSIIAAIAK
KHKSELHTFSVGVEGSKDINAARLVSDYLGTIHHEYLITPKEAIAKLPEIIYALESFDQD
LVRSAIPCYFTSRLAAQYVKVILTGEGADELFAGYTYYKDITSDAILHTELRRSVSSLHN
INLQRVDRLTMAHSIEGRVPFLDLKMIELGQKIPAHLKLRGNPPVEKWILRKAFEDILPD
EIVWRKKEQFDEGSGTVDLLVDTLKDIMSEEQAQTYQKQHSHINLRSAEECYYHQIFMDV
FDNPTAILNNVARWADRPVLV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory