SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_009544757.1 NCBI__GCF_000017845.1:WP_009544757.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 11 hits to proteins with known functional sites (download)

P74535 Bifunctional arginine dihydrolase/ornithine cyclodeaminase ArgZ; EC 3.5.3.27; EC 4.3.1.12 from Synechocystis sp. (strain PCC 6803 / Kazusa) (see paper)
80% identity, 100% coverage: 1:703/703 of query aligns to 1:705/705 of P74535

query
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P74535

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N22 (= N22) binding L-arginine; binding L-ornithine; mutation to A: Significant loss of arginine dihydrolase activity.
D65 (= D65) mutation to A: Significant loss of arginine dihydrolase activity.
F68 (= F68) mutation to A: Significant loss of arginine dihydrolase activity.
N71 (= N71) binding L-arginine; binding L-ornithine; mutation to D: Produces equal trace amounts of citrulline and ornithine.; mutation to S: Transforms the enzyme from a dihydrolase to a deiminase.
R90 (= R90) binding L-arginine; binding L-ornithine; mutation to A: Significant loss of arginine dihydrolase activity.
E118 (= E118) mutation to A: Complete loss of arginine dihydrolase activity.
R139 (= R139) binding L-arginine; binding L-ornithine; mutation to A: Significant loss of arginine dihydrolase activity.
Y167 (= Y167) mutation to A: Significant loss of arginine dihydrolase activity.
H168 (= H168) binding L-arginine; binding L-ornithine; mutation to F: Complete loss of arginine dihydrolase activity.
D170 (= D170) binding L-arginine
A258 (= A258) binding L-arginine; binding L-ornithine
C264 (= C264) binding covalent; binding L-ornithine; mutation to S: Complete loss of arginine dihydrolase activity.

Q8YMD9 Bifunctional arginine dihydrolase/ornithine cyclodeaminase AgrE; Arginine-guanidine removing enzyme; EC 3.5.3.27; EC 4.3.1.12 from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) (see paper)
75% identity, 99% coverage: 1:697/703 of query aligns to 1:698/703 of Q8YMD9

query
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Q8YMD9

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C

C

V

V

K

R

H

N

N

Q

V

I

P

P

F

F

V

V

L

L

A

A

G

G

S

S

I

I

R

R

D

D

D
|
D

G

G

P

P

L

L

P

P

D

D

T

T

E

Q

M

M

D

D

L

L

I

I

K

K

A

A

Q

Q

S

E

R

E

Y

Y

A

A

E

K

L

H

I

L

Q

E

G

G

T

A

D

E

M

M

I

I

L

L

M

M

L

L

S

S

T
x
S

M
|
M

L
|
L

H
|
H

S

S

I

I

G

G

V

V

G

G

N

N

M

M

T

T

P

P

A

A

G

G

V

V

K

K

M

M

V

V

C

C

V

V

D
|
D

I

I

N

N

P

P

A

A

V

V

T

T

K

K

L

L

S

S

D

D

R

R

G

G

S

S

V

I

E

E

S

S

V

V

G

G

I

V

V

V

T

T

D
|
D

V
|
V

G

G

L

L

F

F

L

L

S

S

L

L

V

T

Q

Q

L

L

D

D

K

K

L

L

T

T

H

S

P

P

Y

Y

N22 (= N22) binding L-arginine; binding L-ornithine; mutation to A: Loss of arginine dihydrolase activity.
D65 (= D65) binding L-arginine; mutation to A: Shows residual arginine dihydrolase activity.
N71 (= N71) binding L-arginine; mutation N->A,D: Shows residual arginine dihydrolase activity.
R90 (= R90) binding L-arginine; binding L-ornithine; mutation to A: Loss of arginine dihydrolase activity.
E118 (= E118) mutation to A: Loss of arginine dihydrolase activity.
D122 (= D122) mutation D->A,N: Loss of arginine dihydrolase activity.
R139 (= R139) binding L-arginine; binding L-ornithine; mutation to A: Shows residual arginine dihydrolase activity.
Y167 (= Y167) mutation to F: Retains 9% of arginine dihydrolase activity.
H168 (= H168) binding L-ornithine; mutation to A: Loss of arginine dihydrolase activity.
D170 (= D170) binding L-arginine; mutation D->A,N: Loss of arginine dihydrolase activity.
N219 (= N219) mutation to A: Loss of arginine dihydrolase activity.
A258 (= A258) binding L-arginine
C264 (= C264) binding L-ornithine; mutation to A: Loss of arginine dihydrolase activity.
N524 (= N523) binding NAD(+)
A525 (= A524) binding NAD(+)
D603 (= D602) binding NAD(+)
S635 (≠ T634) binding NAD(+)
M636 (= M635) binding NAD(+)
L637 (= L636) binding NAD(+)
H638 (= H637) binding NAD(+)
D656 (= D655) binding NAD(+)
D679 (= D678) binding NAD(+)
V680 (= V679) binding NAD(+)

6lrgB Crystal structure of the ternary complex of agre with ornithine and NAD+ (see paper)
75% identity, 98% coverage: 5:694/703 of query aligns to 2:681/681 of 6lrgB

query
sites
6lrgB

I

I

R

R

I

F

L

L

M

M

C

C

A

P

P

P

D

D

H

H

Y

Y

D

D

V

V

D

D

Y

Y

V

V

I
|
I

N

N

P

P

W

W

M

M

E

E

G

G

N

N

I

I

H

H

K

K

S

S

T

S

R

R

D

D

R

R

S

A

V

V

E

E

Q

Q

W

W

N

Q

Q

G

L

L

Y

Y

H

Q

A

I

I

L

K

K

E

E

R

H

A

A

I

I

V

V

E

D

L

L

V

V

E

T

P

P

Q

Q

K

K

G

G

W

W

P

P

D
|
D

M

L

V

V

F
|
F

T

T

A

A

N
|
N

A

A

G

G

L

L

V

V

L

L

G

G

D

D

N

N

A

V

I

V

V

L

S

S

R

R

F

F

Y

L

H

H

K

K

E

E

R
|
R

Q

Q

G

G

E

E

P

P

Y

Y

F

F

K

K

E

E

W

W

F

F

A

E

A

G

N

N

G

G

F

Y

N

T

V

V

Y

Y

E

E

L

L

P

P

K

K

D

D

L

L

P

P

F

F

E

E

G

G

A

A

G

G

D

D

A

A

L

L

F

L

D

D

R

R

E

E

G

G

R

R

W

W

L

L

W

W

A

A

G

G

Y

Y

G

G

F

F

R
|
R

S

S

E

E

L

L

D

D

S

S

H

H

P

P

Y

Y

I

L

A

A

Q

K

W

W

L

L

D

D

T

I

E

E

V

V

L

L

S

S

L

L

R

R

L

L

I

I

D

D

E

E

R

R

F

F

Y
|
Y

H
|
H

L

L

D

D

T

T

C

C

F

F

C

C

P

P

L

L

T

A

G

N

G

G

Y

Y

L

L

Y

Y

P

P

P

G

A

A

F

F

D

D

A

S

Y

Y

S

S

N

N

R

R

L

L

I

I

E

E

L

M

R

R

I

V

P

A

Q

P

D

E

K

K

R

R

I

I

V

A

V

I

E

A

E

E

P

A

D

D

A

A

V

V

K

N

F

F

A

A

C

C

N

N

A

T

V

V

N

N

I

V

N

E

H

S

T

I

I

V

I

I

M

M

N

N

Q

K

I

A

S

S

D

D

S

A

L

L

K

K

Q

Q

R

S

L

L

N

T

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G

V

V

G

G

F

F

E

Q

V

V

V

L

Q

E

T

T

P

P

L

L

S

T

E

E

F

F

L

L

K

K

A
|
A

G

G

A

A

K

K

C
|
C

L

L

T

T

L

L

R

R

V

V

N

T

E

E

P

P

V

V

L

R

E

D

E

E

V

V

H

H

A

A

S

N

T

V

P

Y

V

V

E

E

S

S

R

R

T

I

I

I

Q

R

L

I

T

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G

G

H

H

L

L

D

D

A

S

G

G

I

L

M

I

N

N

K

R

A

A

L

L

D

D

M

M

I

I

V

V

E

D

N

T

G

G

S

S

F

F

K

Q

V

V

L

L

N

N

F

F

E

N

L

L

G

G

L

E

E

Q

R

R

Q

Q

S

S

T

T

S

S

A

A

E

E

V

V

R

K

V

V

S

S

A

A

P

P

D

S

H

H

D

E

V

V

M

M

E

E

I

I

M

I

T

S

Q

L

L

L

I

I

D

D

L

L

G

G

A

A

V

V

A

D

P

L

P

P

Q

Q

E

D

V

E

C

R

D

D

I

A

N

K

T

L

E

E

T

P

V

V

T

I

K

Q

A

D

G

G

V

V

A

A

P

P

D

D

F

F

Y

Y

V

V

S

S

T

T

I

I

Y

Y

P

P

T

T

E

E

V

V

R

R

V

I

N

N

C

G

E

Q

W

W

V

I

K

K

V

V

Q

E

N

N

Q

Q

R

R

M

M

D

D

A

G

A

A

I

I

V

A

V

I

-

T

-

Q

D

T

P

P

Q

N

K

G

M

L

T

L

A

A

E

Q

C

C

K

K

L

I

L

L

R

R

D

D

L

L

A

K

V

A

G

G

D

E

R

Q

V

V

M

I

V

V

G

D

F

V

D

L

G

G

I

I

R

R

T

T

V

I

R

R

Q

K

V

T

E

E

D

S

R

R

D

E

Q

Q

R

R

N

-

G

-

K

-

E

-

F

-

T

-

F

-

M

-

G

-

S

-

G

-

V

V

S

S

E

E

R

R

V

V

E

E

L

L

L

V

V

V

E

E

Q

Q

I

V

A

A

W

W

E

E

M

L

R

R

Q

K

V

I

R

R

D

D

Q

A

G

G

K

K

V

V

T

T

A

A

G

G

P

P

V

V

I

I

H

H

T

T

G

G

A

G

Q

E

H

H

L

L

A

S

K

R

L

L

I

I

R

R

E

E

G

G

Y

Y

V

V

H

Q

A

A

L

L

G

G

N

N

A

A

I

I

A

A

V

V

H

H

D

D

I

I

E

E

Q

Q

A

N

M

M

G

G

T

T

S

S

L

L

G

G

V

V

D

D

M

M

Q

K

R

R

G

G

I

V

P

A

V

V

S

R

G

G

H

H

R

R

H

H

L

L

K

K

V

V

I

I

N

N

T

T

V

I

R

R

Y

H

G

G

S

S

I

I

A

A

Q

K

A

G

V

V

E

E

Q

S

G

G

A

I

I

I

S

R

K

S

G

G

V

V

M

M

Y

Y

E

E

C

C

V

V

K

R

H

N

N

Q

V

I

P

P

F

F

V

V

L

L

A

A

G

G

S

S

I

I

R

R

D

D

G

G

P

P

L

L

P

P

D

D

T

T

E

Q

M

M

D

D

L

L

I

I

K

K

A

A

Q

Q

S

E

R

E

Y

Y

A

A

E

K

L

H

I

L

Q

E

G

G

T

A

D

E

M

M

I

I

L

L

M

M

L

L

S

S

T

S

M

M

L

L

H

H

S

S

I

I

G

G

V

V

G

G

N

N

M

M

T

T

P

P

A

A

G

G

V

V

K

K

M

M

V

V

C

C

V

V

D

D

I

I

N

N

P

P

A

A

V

V

T

T

K

K

L

L

S

S

D

D

R

R

G

G

S

S

V

I

E

E

S

S

V

V

G

G

I

V

V

V

T

T

D

D

V

V

G

G

L

L

F

F

L

L

S

S

L

L

V

T

Q

Q

L

L

D

D

K

K

L

L

T

T

binding L-ornithine: I18 (= I21), D62 (= D65), F65 (= F68), N68 (= N71), R87 (= R90), R136 (= R139), Y164 (= Y167), H165 (= H168), A255 (= A258), C261 (= C264)

6lrgA Crystal structure of the ternary complex of agre with ornithine and NAD+ (see paper)
74% identity, 99% coverage: 2:697/703 of query aligns to 1:678/678 of 6lrgA

query
sites
6lrgA

T

T

D

S

P

R

I

I

R

R

I

F

L

L

M

M

C

C

A

P

P

P

D

D

H

H

Y

Y

D

D

V

V

D

D

Y

Y

V

V

I
|
I

N

N

P

P

W

W

M

M

E

E

G

G

N

N

I

I

H

H

K

K

S

S

T

S

R

R

D

D

R

R

S

A

V

V

E

E

Q

Q

W

W

N

Q

Q

G

L

L

Y

Y

H

Q

A

I

I

L

K

K

E

E

R

H

A

A

I

I

V

V

E

D

L

L

V

V

E

T

P

P

Q

Q

K

K

G

G

W

W

P

P

D
|
D

M

L

V

V

F
|
F

T

T

A

A

N
|
N

A

A

G

G

L

L

V

V

L

L

G

G

D

D

N

N

A

V

I

V

V

L

S

S

R

R

F

F

Y

L

H

H

K

K

E

E

R
|
R

Q

Q

G

G

E

E

P

P

Y

Y

F

F

K

K

E

E

W

W

F

F

A

E

A

G

N

N

G

G

F

Y

N

T

V

V

Y

Y

E

E

L

L

P

P

K

K

D

D

L

L

P

P

F

F

E

E

G

G

A

A

G

G

D

D

A

A

L

L

F

L

D

D

R

R

E

E

G

G

R

R

W

W

L

L

W

W

A

A

G

G

Y

Y

G

G

F

F

R
|
R

S

S

E

E

L

L

D

D

S

S

H

H

P

P

Y

Y

I

L

A

A

Q

K

W

W

L

L

D

D

T

I

E

E

V

V

L

L

S

S

L

L

R

R

L

L

I

I

D

D

E

E

R

R

F

F

Y
|
Y

H
|
H

L

L

D

D

T

T

C

C

F

F

C

C

P

P

L

L

T

A

G

N

G

G

Y

Y

L

L

Y

Y

P

P

P

G

A

A

F

F

D

D

A

S

Y

Y

S

S

N

N

R

R

L

L

I

I

E

E

L

M

R

R

I

V

P

A

Q

P

D

E

K

K

R

R

I

I

V

A

V

I

E

A

E

E

P

A

D

D

A

A

V

V

K

N

F

F

A

A

C

C

N

N

A

T

V

V

N

N

I

V

N

E

H

S

T

I

I

V

I

I

M

M

N

N

Q

K

I

A

S

S

D

D

S

A

L

L

K

K

Q

Q

R

S

L

L

N

T

E

G

V

V

G

G

F

F

E

Q

V

V

V

L

Q

E

T

T

P

P

L

L

S

T

E

E

F

F

L

L

K

K

A

A

G

G

A

A

K

K

C
|
C

L

L

T

T

L

L

R

R

V

V

N

T

E

E

P

P

V

V

L

R

E

D

E

E

V

V

H

H

A

A

S

N

T

V

P

Y

V

V

E

E

S

S

R

R

T

I

I

I

Q

R

L

I

T

E

G

G

H

H

L

L

D

D

A

S

G

G

I

L

M

I

N

N

K

R

A

A

L

L

D

D

M

M

I

I

V

V

E

D

N

T

G

G

S

S

F

F

K

Q

V

V

L

L

N

N

F

F

E

N

L

L

G

G

L

E

E

Q

R

R

Q

Q

S

S

T

T

S

S

A

A

E

E

V

V

R

K

V

V

S

S

A

A

P

P

D

S

H

H

D

E

V

V

M

M

E

E

I

I

M

I

T

S

Q

L

L

L

I

I

D

D

L

L

G

G

A

A

V

V

A

D

P

L

P

P

Q

Q

E

D

V

E

C

R

D

D

I

A

N

K

T

L

E

E

T

P

V

V

T

I

K

Q

A

D

G

G

V

V

A

A

P

P

D

D

F

F

Y

Y

V

V

S

S

T

T

I

I

Y

Y

P

P

T

T

E

E

V

V

R

R

V

I

N

N

C

G

E

Q

W

W

V

I

K

K

V

V

Q

E

N

N

Q

Q

R

R

M

M

D

D

A

G

A

A

I

I

V

A

V

I

-

T

-

Q

D

T

P

P

Q

N

K

G

M

L

T

L

A

A

E

Q

C

C

K

K

L

I

L

L

R

R

D

D

L

L

A

K

V

A

G

G

D

E

R

Q

V

V

M

I

V

V

G

D

F

V

D

L

G

G

I

I

R

R

T

T

V

I

R

R

Q

-

V

-

E

-

D

-

R

-

D

-

Q

-

R

-

N

-

G

-

K

-

E

-

F

-

T

-

F

-

M

-

G

-

S

-

G

-

V

V

S

S

E

E

R

R

V

V

E

E

L

L

L

V

V

V

E

E

Q

Q

I

V

A

A

W

W

E

E

M

L

R

R

Q

K

V

I

R

R

D

D

Q

A

G

G

K

K

V

V

T

T

A

A

G

G

P
|
P

V
|
V

V

V

I

I

H

H

T

T

G

G

A

G

Q

E

H

H

L

L

A

S

K

R

L

L

I

I

R

R

E

E

G

G

Y

Y

V

V

H

Q

A

A

L

L

G

G

G
|
G

N
|
N

A
|
A

I

I

A

A

V

V

H

H

D

D

I

I

E

E

Q

Q

A

N

M

M

G

G

T

T

S

S

L

L

G

G

V

V

D

D

M

M

Q

K

R

R

G

G

I

V

P

A

V

V

S

R

G

G

H

H

R

R

H

H

H
|
H

L

L

K

K

V

V

I

I

N

N

T

T

V

I

R

R

Y

H

G

G

S

S

I

I

A

A

Q

K

A

G

V

V

E

E

Q

S

G

G

A

I

I

I

S

R

K

S

G

G

V

V

M

M

Y

Y

E

E

C

C

V

V

K

R

H

N

N

Q

V

I

P

P

F

F

V

V

L

L

A

A

G

G

S
|
S

I

I

R
|
R

D
|
D

G

G

P

P

L

L

P

P

D

D

T

T

E

Q

M

M

D

D

L

L

I

I

K

K

A

A

Q

Q

S

E

R

E

Y

Y

A

A

E

K

L

H

I

L

Q

E

G

G

T

A

D

E

M

M

I

I

L

L

M

M

L

L

S
|
S

T
x
S

M
|
M

L

L

H
|
H

S

S

I

I

G

G

V

V

G

G

N

N

M

M

T

T

P

P

A

A

G

G

V

V

K

K

M

M

V

V

C

C

V

V

D
|
D

I
|
I

N

N

P

P

A

A

V

V

T

T

K

K

L

L

S

S

D

D

R

R

G

G

S

S

V

I

E

E

S

S

V

V

G

G

I

V

V

V

T

T

D
|
D

V
|
V

G

G

L

L

F

F

L

L

S

S

L

L

V

T

Q

Q

L

L

D

D

K

K

L

L

T

T

H

S

P

P

Y

Y

binding nicotinamide-adenine-dinucleotide: P477 (= P496), V478 (= V497), G503 (= G522), N504 (= N523), A505 (= A524), H537 (= H556), S579 (= S598), R581 (= R600), D582 (= D601), D583 (= D602), S614 (= S633), S615 (≠ T634), M616 (= M635), H618 (= H637), D636 (= D655), I637 (= I656), D659 (= D678), V660 (= V679)
binding L-ornithine: I20 (= I21), D64 (= D65), F67 (= F68), N70 (= N71), R89 (= R90), R138 (= R139), Y166 (= Y167), H167 (= H168), C263 (= C264)

6lrhA Crystal structure of the binary complex of agre c264a mutant with l- arginine (see paper)
73% identity, 99% coverage: 2:696/703 of query aligns to 1:675/675 of 6lrhA

query
sites
6lrhA

T

T

D

S

P

R

I

I

R

R

I

F

L

L

M

M

C

C

A

P

P

P

D

D

H

H

Y

Y

D

D

V

V

D

D

Y

Y

V

V

I
|
I

N

N

P

P

W

W

M

M

E

E

G

G

N

N

I

I

H

H

K

K

S

S

T

S

R

R

D

D

R

R

S

A

V

V

E

E

Q

Q

W

W

N

Q

Q

G

L

L

Y

Y

H

Q

A

I

I

L

K

K

E

E

R

H

A

A

I

I

V

V

E

D

L

L

V

V

E

T

P

P

Q

Q

K

K

G

G

W

W

P

P

D
|
D

M

L

V

V

F
|
F

T

T

A

A

N
|
N

A

A

G

G

L

L

V

V

L

L

G

G

D

D

N

N

A

V

I

V

V

L

S

S

R

R

F

F

Y

L

H

H

K

K

E

E

R
|
R

Q

Q

G

G

E

E

P

P

Y

Y

F

F

K

K

E

E

W

W

F

F

A

E

A

G

N

N

G

G

F

Y

N

T

V

V

Y

Y

E

E

L

L

P

P

K

K

D

D

L

L

P

P

F

F

E

E

G

G

A

A

G

G

D

D

A

A

L

L

F

L

D

D

R

R

E

E

G

G

R

R

W

W

L

L

W

W

A

A

G

G

Y

Y

G

G

F

F

R
|
R

S

S

E

E

L

L

D

D

S

S

H

H

P

P

Y

Y

I

L

A

A

Q

K

W

W

L

L

D

D

T

I

E

E

V

V

L

L

S

S

L

L

R

R

L

L

I

I

D

D

E

E

R

R

F

F

Y
|
Y

H
|
H

L

L

D
|
D

T

T

C

C

F

F

C

C

P

P

L

L

T

A

G

N

G

G

Y

Y

L

L

Y

Y

P

P

P

G

A

A

F

F

D

D

A

S

Y

Y

S

S

N

N

R

R

L

L

I

I

E

E

L

M

R

R

I

V

P

A

Q

P

D

E

K

K

R

R

I

I

V

A

V

I

E

A

E

E

P

A

D

D

A

A

V

V

K

N

F

F

A

A

C

C

N

N

A

T

V

V

N

N

I

V

N

E

H

S

T

I

I

V

I

I

M

M

N

N

Q

K

I

A

S

S

D

D

S

A

L

L

K

K

Q

Q

R

S

L

L

N

T

E

G

V

V

G

G

F

F

E

Q

V

V

V

L

Q

E

T

T

P

P

L

L

S

T

E

E

F

F

L

L

K

K

A
|
A

G

G

A

A

K

K

C
x
A

L

L

T

T

L

L

R

R

V

V

N

T

E

E

P

P

V

V

L

R

E

D

E

E

V

V

H

H

A

A

S

N

T

V

P

Y

V

V

E

E

S

S

R

R

T

I

I

I

Q

R

L

I

T

E

G

G

H

H

L

L

D

D

A

S

G

G

I

L

M

I

N

N

K

R

A

A

L

L

D

D

M

M

I

I

V

V

E

D

N

T

G

G

S

S

F

F

K

Q

V

V

L

L

N

N

F

F

E

N

L

L

G

G

L

E

E

Q

R

R

Q

Q

S

S

T

T

S

S

A

A

E

E

V

V

R

K

V

V

S

S

A

A

P

P

D

S

H

H

D

E

V

V

M

M

E

E

I

I

M

I

T

S

Q

L

L

L

I

I

D

D

L

L

G

G

A

A

V

V

A

D

P

L

P

P

Q

Q

E

D

V

E

C

R

D

D

I

A

N

K

T

L

E

E

T

P

V

V

T

I

K

Q

A

D

G

G

V

V

A

A

P

P

D

D

F

F

Y

Y

V

V

S

S

T

T

I

I

Y

Y

P

P

T

T

E

E

V

V

R

R

V

I

N

N

C

G

E

Q

W

W

V

I

K

K

V

V

Q

E

N

N

Q

Q

R

R

M

M

D

D

A

G

A

A

I

I

V

A

V

I

-

T

-

Q

D

T

P

P

Q

N

K

G

M

L

T

L

A

A

E

Q

C

C

K

K

L

I

L

L

R

R

D

D

L

L

A

K

V

A

G

G

D

E

R

Q

V

V

M

I

V

V

G

D

F

V

D

L

G

G

I

I

R

R

T

T

V

I

R

R

Q

-

V

-

E

-

D

-

R

-

D

-

Q

-

R

-

N

-

G

-

K

-

E

-

F

-

T

-

F

-

M

-

G

-

S

-

G

-

V

V

S

S

E

E

R

R

V

V

E

E

L

L

L

V

V

V

E

E

Q

Q

I

V

A

A

W

W

E

E

M

L

R

R

Q

K

V

I

R

R

D

D

Q

A

G

G

K

K

V

V

T

T

A

A

G

G

P

P

V

V

I

I

H

H

T

T

G

G

A

G

Q

E

H

H

L

L

A

S

K

R

L

L

I

I

R

R

E

E

G

G

Y

Y

V

V

H

Q

A

A

L

L

G

G

N

N

A

A

I

I

A

A

V

V

H

H

D

D

I

I

E

E

Q

Q

A

N

M

M

G

G

T

T

S

S

L

L

G

G

V

V

D

D

M

M

Q

K

R

R

G

G

I

V

P

A

V

V

S

R

G

G

H

H

R

R

H

H

L

L

K

K

V

V

I

I

N

N

T

T

V

I

R

R

Y

H

G

G

S

S

I

I

A

A

Q

K

A

G

V

V

E

E

Q

S

G

G

A

I

I

I

S

R

K

S

G

G

V

V

M

M

Y

Y

E

E

C

C

V

V

K

R

H

N

N

Q

V

I

P

P

F

F

V

V

L

L

A

A

G

G

S

S

I

I

R

R

D

D

G

G

P

P

L

L

P

P

D

D

T

T

E

Q

M

M

D

D

L

L

I

I

K

K

A

A

Q

Q

S

E

R

E

Y

Y

A

A

E

K

L

H

I

L

Q

E

G

G

T

A

D

E

M

M

I

I

L

L

M

M

L

L

S

S

T

S

M

M

L

L

H

H

S

S

I

I

G

G

V

V

G

G

N

N

M

M

T

T

P

P

A

A

G

G

V

V

K

K

M

M

V

V

C

C

V

V

D

D

I

I

N

N

P

P

A

A

V

-

T

T

K

K

L

L

S

S

D

D

R

R

G

G

S

S

V

I

E

E

S

S

V

V

G

G

I

V

V

V

T

T

D

D

V

V

G

G

L

L

F

F

L

L

S

S

L

L

V

T

Q

Q

L

L

D

D

K

K

L

L

T

T

H

S

P

P

binding arginine: I20 (= I21), D64 (= D65), F67 (= F68), N70 (= N71), R89 (= R90), R138 (= R139), Y166 (= Y167), H167 (= H168), D169 (= D170), A257 (= A258), A263 (≠ C264)

6jv0A Crystal structure of n-terminal domain of argz, bound to product, an arginine dihydrolase from the ornithine-ammonia cycle in cyanobacteria (see paper)
81% identity, 39% coverage: 3:274/703 of query aligns to 1:272/273 of 6jv0A

query
sites
6jv0A

D

D

P

D

I

I

R

R

I

I

L

L

M

M

C

C

A

P

P

P

D

D

H

H

Y

Y

D

D

V

V

D

D

Y

Y

V

V

I
|
I

N

N

P

P

W

W

M
|
M

E

E

G

G

N

N

I

I

H

H

K

K

S

S

T

S

R

Q

D

E

R

R

S

A

V

V

E

E

Q

Q

W

W

N

K

Q

K

L

L

Y

H

H

Q

A

T

I

I

K

K

E

E

R

C

A

A

I

I

V

V

E

D

L

L

V

V

E

K

P

P

Q

A

K

K

G

G

W

W

P

P

D
|
D

M

M

V

V

F
|
F

T

T

A

A

N
|
N

A

A

G

G

L

L

V

V

L

L

G

G

D

E

N

N

A

V

I

V

V

L

S

S

R

R

F

F

Y

Y

H

H

K

K

E

E

R
|
R

Q

Q

G

G

E

E

P

P

Y

Y

F

F

K

K

E

A

W

W

F

F

A

E

N

N

G

G

F

F

N

T

V

V

Y

Y

E

E

L

L

P

P

K

Q

D

D

L

L

P

P

F

F

E

E

G

G

A

A

G

G

D

D

A

A

L

L

F

F

D

D

R

R

E

E

G

G

R

R

W

W

L

L

W

W

A

A

G

G

Y

Y

G

G

F

F

R
|
R

S

S

E

E

L

L

D

D

S

S

H

H

P

P

Y

Y

I

I

A

A

Q

K

W

W

L

L

D

D

T

T

E

E

V

V

L

V

S

S

L

L

R

R

L

L

I

I

D

D

E

E

R

R

F

F

Y
|
Y

H
|
H

L

L

D

D

T

T

C

C

F

F

C

C

P

P

L

L

T

S

G

G

Y

Y

L

L

Y

Y

P

P

A

A

F

F

D

D

A

A

Y

Y

S

S

N

N

R

R

L

V

I

I

E

E

L

M

R

R

I

I

P

P

Q

P

D

E

K

K

R

R

I

I

V

I

V

V

E

E

P

L

D

D

A

A

V

V

K

N

F

F

A

A

C

C

N

N

A

A

V

V

N

N

I

V

N

N

H

D

T

I

I

I

M

M

N

N

Q

L

I

V

S

S

D

R

S

T

L

L

K

K

Q

E

R

K

L

L

N

A

E

E

V

A

G

G

F

F

E

K

V

V

V

R

Q

E

T

T

P

P

L

L

S

T

E

E

F

F

L

L

K

K

A
|
A

G

G

A

A

K

K

C
|
C

L

L

T

T

L

L

R

R

V

V

N

T

E

E

P

P

V

I

L

L

binding L-ornithine: I19 (= I21), M23 (= M25), D63 (= D65), F66 (= F68), N69 (= N71), R88 (= R90), R137 (= R139), Y165 (= Y167), H166 (= H168), A256 (= A258), C262 (= C264)

6juzA Crystal structure of n-terminal domain of argz(n71s) covalently bond to a reaction intermediate (see paper)
81% identity, 38% coverage: 5:274/703 of query aligns to 2:271/272 of 6juzA

query
sites
6juzA

I

I

R

R

I

I

L

L

M

M

C

C

A

P

P

P

D

D

H

H

Y

Y

D

D

V

V

D

D

Y

Y

V

V

I
|
I

N

N

P

P

W

W

M

M

E

E

G

G

N

N

I

I

H

H

K

K

S

S

T

S

R

Q

D

E

R

R

S

A

V

V

E

E

Q

Q

W

W

N

K

Q

K

L

L

Y

H

H

Q

A

T

I

I

K

K

E

E

R

C

A

A

I

I

V

V

E

D

L

L

V

V

E

K

P

P

Q

A

K

K

G

G

W

W

P

P

D
|
D

M

M

V

V

F
|
F

T

T

A

A

N

S

A

A

G

G

L

L

V

V

L

L

G

G

D

E

N

N

A

V

I

V

V

L

S

S

R

R

F

F

Y

Y

H

H

K

K

E

E

R
|
R

Q

Q

G

G

E

E

P

P

Y

Y

F

F

K

K

E

A

W

W

F

F

A

E

N

N

G

G

F

F

N

T

V

V

Y

Y

E

E

L

L

P

P

K

Q

D

D

L

L

P

P

F

F

E

E

G

G

A

A

G

G

D

D

A

A

L

L

F

F

D

D

R

R

E

E

G

G

R

R

W

W

L

L

W

W

A

A

G

G

Y

Y

G

G

F

F

R
|
R

S

S

E

E

L

L

D

D

S

S

H

H

P

P

Y

Y

I

I

A

A

Q

K

W

W

L

L

D

D

T

T

E

E

V

V

L

V

S

S

L

L

R

R

L

L

I

I

D

D

E

E

R

R

F

F

Y
|
Y

H
|
H

L

L

D
|
D

T

T

C

C

F

F

C

C

P

P

L

L

T

S

G

G

Y

Y

L

L

Y

Y

P

P

A

A

F

F

D

D

A

A

Y

Y

S

S

N

N

R

R

L

V

I

I

E

E

L

M

R

R

I

I

P

P

Q

P

D

E

K

K

R

R

I

I

V

I

V

V

E

E

P

L

D

D

A

A

V

V

K

N

F

F

A

A

C

C

N

N

A

A

V

V

N

N

I

V

N

N

H

D

T

I

I

I

M

M

N

N

Q

L

I

V

S

S

D

R

S

T

L

L

K

K

Q

E

R

K

L

L

N

A

E

E

V

A

G

G

F

F

E

K

V

V

V

R

Q

E

T

T

P

P

L

L

S

T

E

E

F

F

L

L

K

K

A
|
A

G

G

A

A

K

K

C
|
C

L

L

T

T

L

L

R

R

V

V

N

T

E

E

P

P

V

I

L

L

binding arginine: I18 (= I21), D62 (= D65), F65 (= F68), R87 (= R90), R136 (= R139), Y164 (= Y167), H165 (= H168), D167 (= D170), A255 (= A258), C261 (= C264)

Q9I4E3 N(G),N(G)-dimethylarginine dimethylaminohydrolase; DDAH; Dimethylarginine dimethylaminohydrolase; Dimethylargininase; EC 3.5.3.18 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 2 papers)
22% identity, 35% coverage: 20:268/703 of query aligns to 14:253/254 of Q9I4E3

query
sites
Q9I4E3

V

L

I

V

N

D

P

G

W

L

M

T

E

S

G

S

N

H

I

L

H

G

K

K

S

P

T

D

R

Y

D

A

R

K

S

A

V

L

E

E

Q

Q

W

H

N

N

Q

A

L

Y

Y

I

H

R

A

A

I

L

K

Q

E

T

R

C

A

D

I

V

-

D

V

I

E

T

L

L

V

L

E

P

P

P

Q

D

K

E

G

R

W

F

P

P

D

D

M

S

V

V

F

F

T

V

A

E

N

D

A

P

G

V

L

L

V

C

L

T

G

S

D

R

N

C

A

A

I

I

V

I

S

T

R

R

F

P

Y

G

H

A

K

E

E

S

R

R

Q

R

G

G

E

E

E

T

P

E

Y

I

F

I

K

E

E

E

W

T

-

V

-

Q

-

R

F

F

A

Y

A

P

N

G

G

K

F

V

N

E

V

R

Y

I

E

E

L

A

P

P

K

G

D

T

L

V

P
x
E

F

-

E

-

G

-

A

A

G

G

D

D

A

I

L

M

F

M

D

-

R

-

E

V

G

G

R

D

W

H

L

F

W

Y

A

I

G

G

Y

E

G

S

F

A

R

R

S

T

E

N

L

A

D

E

S

G

H

A

P

R

Y

Q

I

M

A

I

Q

A

W

I

L

L

D

E

T

K

E

H

V

G

L

L

S

S

L

G

R

S

L

V

I

V

D

R

-

L

E

E

R

K

F

V

Y

L

H
|
H

L

L

D

K

T

T

C

G

F

L

C

A

P

Y

L

L

T

E

G

H

G

N

Y

N

L

L

-

A

-

G

-

E

-

F

Y

V

Y

S

P

K

P

P

A

E

F

F

D

Q

A

D

Y

F

S

N

N

-

R

-

L

-

I

-

E

-

L

-

R

-

I

-

P

-

Q

-

D

-

K

-

R

-

I

-

V

I

E

E

E

I

P

P

D

E

A

E

V

E

K

S

F

Y

A

A

C

A

N

N

A

C

V

I

N

W

I

V

N

N

H

E

T

R

I

V

I

I

M

M

N

P

Q

A

I

G

S

Y

D

P

S

R

L

T

K

R

Q

E

R

K

L

I

N

A

E

R

V

L

G

G

F

Y

E

R

V

V

V

I

Q

E

T

V

P

D

L

T

S

S

E

E

F

Y

L

R

K

K

A

I

G

D

G

G

A

G

A

V

K

S

C
|
C

L

M

T

S

L

L

R

R

E114 (≠ P116) mutation to Q: Abolishes enzyme activity.
H162 (= H168) mutation H->G,F: Abolishes enzyme activity.
C249 (= C264) mutation to S: Abolishes enzyme activity.

3rhyA Crystal structure of the dimethylarginine dimethylaminohydrolase adduct with 4-chloro-2-hydroxymethylpyridine (see paper)
22% identity, 34% coverage: 28:268/703 of query aligns to 16:247/248 of 3rhyA

query
sites
3rhyA

N

D

I

L

H

G

K

K

S

P

T

D

R

Y

D

A

R

K

S

A

V

L

E

E

Q

Q

W

H

N

N

Q

A

L

Y

Y

I

H

R

A

A

I

L

K

Q

E

T

R

C

A

D

I

V

-

D

V

I

E

T

L

L

V

L

E

P

P

P

Q

D

K

E

G

R

W

F

P

P

D

D

M

S

V

V

F
|
F

T

V

A

E

N

D

A

P

G

V

L

L

V

C

L

T

G

S

D

R

N

C

A

A

I

I

V

I

S

T

R

R

F

P

Y

G

H

A

K

E

E

S

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R

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R

G

G

E

E

E

T

P

E

Y

I

F

I

K

E

E

E

W

T

-

V

-

Q

-

R

F

F

A

Y

A

P

N

G

G

K

F

V

N

E

V

R

Y

I

E

E

L

A

P

P

K

G

D

T

L

V

P

E

F

-

E

-

G

-

A

A

G

G

D

D

A

I

L

M

F

M

D

-

R

-

E

V

G

G

R

D

W

H

L

F

W

Y

A

I

G

G

Y

E

G

S

F

A

R

R

S

T

E

N

L

A

D

E

S

G

H

A

P

R

Y

Q

I

M

A

I

Q

A

W

I

L

L

D

E

T

K

E

H

V

G

L

L

S

S

L

G

R

S

L

V

I

V

D

R

-

L

E

E

R

K

F

V

Y

L

H
|
H

L

L

D

K

T

T

C

G

F

L

C

A

P

Y

L

L

T

E

G

H

G

N

Y

N

L

L

-

A

-

G

-

E

-

F

Y

V

Y

S

P

K

P

P

A

E

F

F

D

Q

A

D

Y

F

S

N

N

-

R

-

L

-

I

-

E

-

L

-

R

-

I

-

P

-

Q

-

D

-

K

-

R

-

I

-

V

I

E

E

E

I

P

P

D

E

A

E

V

E

K

S

F

Y

A

A

C

A

N

N

A

C

V

I

N

W

I

V

N

N

H

E

T

R

I

V

I

I

M

M

N

P

Q

A

I

G

S

Y

D

P

S

R

L

T

K

R

Q

E

R

K

L

I

N

A

E

R

V

L

G

G

F

Y

E

R

V

V

V

I

Q

E

T

V

P

D

L

T

S

S

E

E

F

Y

L

R

K

K

A

I

G
x
D

G

G

A

G

A

V

K

S

C
|
C

L

M

T

S

L

L

R

R

binding (4-chloropyridin-2-yl)methanol: F57 (= F68), H156 (= H168), D238 (≠ G259), C243 (= C264)

Q9SMZ4 Alpha-aminoadipic semialdehyde synthase; cAt-LKR/SDH; LKR/SDH; EC 1.5.1.8; EC 1.5.1.9 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
41% identity, 11% coverage: 288:361/703 of query aligns to 482:552/1064 of Q9SMZ4

query
sites
Q9SMZ4

I

V

Q

S

L

L

T

S

G

G

H

H

L

L

L

F

D

D

A

K

G

F

I

L

M

I

N

N

K

E

A

A

L

L

D

D

M

M

I

I

V

E

E

A

N

A

G

G

S

S

F

F

K

H

V

L

L

A

N

K

F

C

E

E

L

L

G

G

L

Q

E

S

R

A

Q

D

S

A

T

E

S

S

A

Y

A

S

E

E

V

L

R

E

V

V

S

G

A

A

P

D

D

D

H

K

D

R

V

V

M

L

E

D

E

Q

I

I

M

I

T

D

Q

S

L

L

I

T

D

R

L

L

G

-

A

-

V

-

A

A

P

N

P

P

Q
x
N

E
|
E

Sites not aligning to the query:

238 modified: Phosphothreonine; mutation T->A,D: No effect on LKR and SDH activity.
407 S→A: No effect on LKR and SDH activity.; S→D: No LKR activity, but no effect on SDH activity.
458 modified: Phosphoserine; S→A: Reduced LKR activity, but no effect on SDH activity.; S→D: No effect on LKR and SDH activity.
551:554 NEDY→IEGR: Loss of LKR activity stimulation by NaCl.

1h70A Ddah from pseudomonas aeruginosa. C249s mutant complexed with citrulline (see paper)
21% identity, 35% coverage: 20:268/703 of query aligns to 15:254/255 of 1h70A

query
sites
1h70A

V

L

I

V

N

D

P

G

W
x
L

M

T

E

S

G

S

N

H

I

L

H

G

K

K

S

P

T

D

R

Y

D

A

R

K

S

A

V

L

E

E

Q

Q

W

H

N

N

Q

A

L

Y

Y

I

H

R

A

A

I

L

K

Q

E

T

R

C

A

D

I

V

-

D

V

I

E

T

L

L

V

L

E

P

P

P

Q

D

K

E

G

R

W

F

P

P

D
|
D

M

S

V

V

F
|
F

T

V

A
x
E

N
x
D

A

P

G

V

L

L

V

C

L

T

G

S

D

R

N

C

A

A

I

I

V

I

S

T

R

R

F

P

Y

G

H

A

K

E

E

S

R
|
R

Q

R

G

G

E

E

E

T

P

E

Y

I

F

I

K

E

E

E

W

T

-

V

-

Q

-

R

F

F

A

Y

A

P

N

G

G

K

F

V

N

E

V

R

Y

I

E

E

L

A

P

P

K

G

D

T

L

V

P

E

F

-

E

-

G

-

A

A

G

G

D

D

A

I

L

M

F

M

D

-

R

-

E

V

G

G

R

D

W

H

L

F

W

Y

A

I

G

G

Y

E

G

S

F

A

R
|
R

S

T

E

N

L

A

D

E

S

G

H

A

P

R

Y

Q

I

M

A

I

Q

A

W

I

L

L

D

E

T

K

E

H

V

G

L

L

S

S

L

G

R

S

L

V

I

V

D

R

-

L

E

E

R

K

F

V

Y

L

H
|
H

L

L

D

K

T

T

C

G

F

L

C

A

P

Y

L

L

T

E

G

H

G

N

Y

N

L

L

-

A

-

G

-

E

-

F

Y

V

Y

S

P

K

P

P

A

E

F

F

D

Q

A

D

Y

F

S

N

N

-

R

-

L

-

I

-

E

-

L

-

R

-

I

-

P

-

Q

-

D

-

K

-

R

-

I

-

V

I

E

E

E

I

P

P

D

E

A

E

V

E

K

S

F

Y

A

A

C

A

N

N

A

C

V

I

N

W

I

V

N

N

H

E

T

R

I

V

I

I

M

M

N

P

Q

A

I

G

S

Y

D

P

S

R

L

T

K

R

Q

E

R

K

L

I

N

A

E

R

V

L

G

G

F

Y

E

R

V

V

V

I

Q

E

T

V

P

D

L

T

S

S

E

E

F

Y

L

R

K

K

A
x
I

G
x
D

G

G

A

G

A

V

K

S

C
x
S

L

M

T

S

L

L

R

R

binding citrulline: L19 (≠ W24), D61 (= D65), F64 (= F68), E66 (≠ A70), D67 (≠ N71), R86 (= R90), R133 (= R139), H163 (= H168), I244 (≠ A258), D245 (≠ G259), S250 (≠ C264)

Query Sequence

>WP_009544757.1 NCBI__GCF_000017845.1:WP_009544757.1
MTDPIRILMCAPDHYDVDYVINPWMEGNIHKSTRDRSVEQWNQLYHAIKERAIVELVEPQ
KGWPDMVFTANAGLVLGDNAIVSRFYHKERQGEEPYFKEWFAANGFNVYELPKDLPFEGA
GDALFDREGRWLWAGYGFRSELDSHPYIAQWLDTEVLSLRLIDERFYHLDTCFCPLTGGY
LLYYPPAFDAYSNRLIELRIPQDKRIVVEEPDAVKFACNAVNINHTIIMNQISDSLKQRL
NEVGFEVVQTPLSEFLKAGGAAKCLTLRVNEPVLEEVHASTPVESRTIQLTGHLLDAGIM
NKALDMIVENGGSFKVLNFELGLERQSTSAAEVRVSAPDHDVMEEIMTQLIDLGAVAPPQ
EVCDINTETVTKAGVAPDDFYVSTIYPTEVRVNCEWVKVQNQRMDAAIVVDPQKMTAECK
LLRDLAVGDRVMVGFDGIRTVRQVEDRDQRNGKKEFTFMGSGVSSERRVELLVEQIAWEM
RQVRDQGGKVVVTAGPVVIHTGGAQHLAKLIREGYVHALLGGNAIAVHDIEQAMMGTSLG
VDMQRGIPVSGGHRHHLKVINTVRRYGSIAQAVEQGAISKGVMYECVKHNVPFVLAGSIR
DDGPLPDTEMDLIKAQSRYAELIQGTDMILMLSTMLHSIGVGNMTPAGVKMVCVDINPAV
VTKLSDRGSVESVGIVTDVGLFLSLLVQQLDKLTHPYPLVGTV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory