SitesBLAST
Comparing WP_009545077.1 NCBI__GCF_000017845.1:WP_009545077.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
2q5oA X-ray structure of phenylpyruvate decarboxylase in complex with 3- deaza-thdp and phenylpyruvate (see paper)
37% identity, 93% coverage: 7:510/544 of query aligns to 4:499/529 of 2q5oA
- active site: I23 (≠ V26), G25 (= G28), D26 (= D29), F27 (= F30), A28 (≠ V31), E49 (= E51), T72 (= T73), H113 (= H114), H114 (= H115), G116 (≠ V117), R117 (≠ K118), T118 (≠ E119), L119 (≠ F120), R166 (= R167), V258 (≠ A261), N285 (= N288), M373 (≠ V381), A395 (= A406), M397 (≠ L408), D422 (= D433), N449 (= N460), S451 (≠ G462), W452 (≠ Y463), M454 (≠ T465), L455 (≠ E466), F458 (≠ M469)
- binding magnesium ion: D422 (= D433), N449 (= N460), S451 (≠ G462)
- binding 3-phenylpyruvic acid: G25 (= G28), D26 (= D29), R61 (= R63), H113 (= H114), H114 (= H115), R215 (≠ H218), R216 (= R219), M239 (≠ L242), G242 (≠ S245), T284 (≠ V287), L388 (≠ F399), M389 (≠ L400), A390 (≠ S401), M454 (≠ T465)
- binding 2-{4-[(4-amino-2-methylpyrimidin-5-yl)methyl]-3-methylthiophen-2-yl}ethyl trihydrogen diphosphate: P24 (= P27), E49 (= E51), A75 (≠ V76), D375 (= D383), M397 (≠ L408), G421 (= G432), D422 (= D433), G423 (= G434), A424 (= A435), N449 (= N460), S451 (≠ G462), W452 (≠ Y463), E453 (≠ G464), M454 (≠ T465), L455 (≠ E466)
Sites not aligning to the query:
2q5qA X-ray structure of phenylpyruvate decarboxylase in complex with 3- deaza-thdp and 5-phenyl-2-oxo-valeric acid (see paper)
36% identity, 93% coverage: 3:510/544 of query aligns to 1:504/533 of 2q5qA
- active site: I24 (≠ V26), G26 (= G28), D27 (= D29), F28 (= F30), A29 (≠ V31), E50 (≠ Q52), T73 (= T73), H114 (= H114), H115 (= H115), G117 (≠ V117), R118 (≠ K118), T119 (≠ E119), L120 (≠ F120), R167 (= R167), V259 (≠ A261), N286 (= N288), M378 (≠ V381), A400 (= A406), M402 (≠ L408), D427 (= D433), N454 (= N460), S456 (≠ G462), W457 (≠ Y463), M459 (≠ T465), L460 (≠ E466), F463 (≠ M469)
- binding 5-phenyl-2-keto-valeric acid: G26 (= G28), D27 (= D29), R62 (= R63), H114 (= H114), H115 (= H115), R216 (≠ H218), R217 (= R219), M240 (≠ L242), R242 (≠ K244), D284 (= D286), T285 (≠ V287), L373 (≠ I376), L393 (≠ F399), M394 (≠ L400), A395 (≠ S401), A400 (= A406), M459 (≠ T465)
- binding magnesium ion: D427 (= D433), N454 (= N460), S456 (≠ G462)
- binding 2-{4-[(4-amino-2-methylpyrimidin-5-yl)methyl]-3-methylthiophen-2-yl}ethyl trihydrogen diphosphate: P25 (= P27), E50 (≠ Q52), A76 (≠ V76), D380 (= D383), M402 (≠ L408), G426 (= G432), D427 (= D433), G428 (= G434), A429 (= A435), N454 (= N460), S456 (≠ G462), W457 (≠ Y463), E458 (≠ G464), M459 (≠ T465), L460 (≠ E466)
Sites not aligning to the query:
2q5lA X-ray structure of phenylpyruvate decarboxylase in complex with 2-(1- hydroxyethyl)-3-deaza-thdp (see paper)
36% identity, 93% coverage: 3:510/544 of query aligns to 5:507/538 of 2q5lA
- active site: I28 (≠ V26), G30 (= G28), D31 (= D29), F32 (= F30), A33 (≠ V31), E54 (= E51), T77 (= T73), T118 (≠ E119), L119 (≠ F120), R166 (= R167), V258 (≠ A261), N285 (= N288), M381 (≠ V381), A403 (= A406), M405 (≠ L408), D430 (= D433), N457 (= N460), S459 (≠ G462), W460 (≠ Y463), M462 (≠ T465), L463 (≠ E466), F466 (≠ M469)
- binding magnesium ion: D430 (= D433), N457 (= N460), S459 (≠ G462)
- binding 2-{4-[(4-amino-2-methylpyrimidin-5-yl)methyl]-5-[(1r)-1-hydroxyethyl]-3-methyl-2-thienyl}ethyl trihydrogen diphosphate: P29 (= P27), D31 (= D29), E54 (= E51), D383 (= D383), A403 (= A406), M405 (≠ L408), D430 (= D433), G431 (= G434), N457 (= N460), S459 (≠ G462), W460 (≠ Y463), E461 (≠ G464), M462 (≠ T465), L463 (≠ E466)
- binding 2-{4-[(4-amino-2-methylpyrimidin-5-yl)methyl]-5-[(1s)-1-hydroxyethyl]-3-methyl-2-thienyl}ethyl trihydrogen diphosphate: P29 (= P27), D31 (= D29), E54 (= E51), D383 (= D383), A403 (= A406), M405 (≠ L408), G429 (= G432), D430 (= D433), G431 (= G434), A432 (= A435), N457 (= N460), S459 (≠ G462), W460 (≠ Y463), E461 (≠ G464), M462 (≠ T465), L463 (≠ E466)
Sites not aligning to the query:
2nxwA Crystal structure of phenylpyruvate decarboxylase of azospirillum brasilense (see paper)
36% identity, 93% coverage: 3:510/544 of query aligns to 6:504/537 of 2nxwA
- active site: I29 (≠ V26), G31 (= G28), D32 (= D29), F33 (= F30), A34 (≠ V31), E55 (= E51), T78 (= T73), R163 (= R167), V255 (≠ A261), N282 (= N288), M378 (≠ V381), A400 (= A406), M402 (≠ L408), D427 (= D433), N454 (= N460), S456 (≠ G462), W457 (≠ Y463), M459 (≠ T465), L460 (≠ E466), F463 (≠ M469)
- binding magnesium ion: D427 (= D433), N454 (= N460), S456 (≠ G462)
- binding thiamine diphosphate: P30 (= P27), E55 (= E51), D380 (= D383), M402 (≠ L408), G426 (= G432), D427 (= D433), G428 (= G434), A429 (= A435), N454 (= N460), S456 (≠ G462), W457 (≠ Y463), E458 (≠ G464), M459 (≠ T465), L460 (≠ E466)
Sites not aligning to the query:
2q5jA X-ray structure of phenylpyruvate decarboxylase in complex with 3- deaza-thdp (see paper)
36% identity, 93% coverage: 7:510/544 of query aligns to 3:491/523 of 2q5jA
- active site: I22 (≠ V26), G24 (= G28), D25 (= D29), F26 (= F30), A27 (≠ V31), E48 (= E51), T71 (= T73), R150 (= R167), V242 (≠ A261), N269 (= N288), M365 (≠ V381), A387 (= A406), M389 (≠ L408), D414 (= D433), N441 (= N460), S443 (≠ G462), W444 (≠ Y463), M446 (≠ T465), L447 (≠ E466), F450 (≠ M469)
- binding magnesium ion: D414 (= D433), N441 (= N460), S443 (≠ G462)
- binding 2-{4-[(4-amino-2-methylpyrimidin-5-yl)methyl]-3-methylthiophen-2-yl}ethyl trihydrogen diphosphate: P23 (= P27), E48 (= E51), G366 (= G382), D367 (= D383), A387 (= A406), G388 (≠ S407), M389 (≠ L408), G413 (= G432), D414 (= D433), G415 (= G434), A416 (= A435), N441 (= N460), W444 (≠ Y463), E445 (≠ G464), M446 (≠ T465), L447 (≠ E466)
Sites not aligning to the query:
6vgsBBB Alpha-keto acid decarboxylase (see paper)
31% identity, 99% coverage: 6:543/544 of query aligns to 3:540/543 of 6vgsBBB
- active site: V23 (= V26), G25 (= G28), D26 (= D29), Y27 (≠ F30), N28 (≠ V31), E49 (= E51), T71 (= T73), H112 (= H114), H113 (= H115), L115 (≠ V117), A116 (≠ K118), V166 (≠ R167), S282 (≠ N288), Q372 (≠ V381), G397 (≠ A406), I399 (≠ L408), D424 (= D433), N451 (= N460), G453 (= G462), Y454 (= Y463), V456 (≠ T465), E457 (= E466)
- binding magnesium ion: D424 (= D433), N451 (= N460), G453 (= G462)
- binding thiamine diphosphate: P24 (= P27), E49 (= E51), V74 (= V76), T374 (≠ D383), I399 (≠ L408), G423 (= G432), G425 (= G434), S426 (≠ A435), N451 (= N460), G453 (= G462), Y454 (= Y463), T455 (≠ G464), V456 (≠ T465), E457 (= E466)
2vbgA The complex structure of the branched-chain keto acid decarboxylase (kdca) from lactococcus lactis with 2r-1-hydroxyethyl-deazathdp (see paper)
30% identity, 99% coverage: 6:543/544 of query aligns to 8:544/546 of 2vbgA
- active site: V28 (= V26), G30 (= G28), D31 (= D29), Y32 (≠ F30), N33 (≠ V31), N53 (≠ D50), E54 (= E51), T76 (= T73), F115 (≠ L112), V116 (≠ L113), H117 (= H114), H118 (= H115), L120 (≠ V117), A121 (≠ K118), V171 (= V170), K259 (≠ A261), S286 (≠ N288), E375 (≠ D380), Q376 (≠ V381), G401 (≠ A406), I403 (≠ L408), D428 (= D433), N455 (= N460), G457 (= G462), Y458 (= Y463), V460 (≠ T465), E461 (= E466), K527 (≠ P526)
- binding magnesium ion: D428 (= D433), N455 (= N460), G457 (= G462)
- binding 2-{4-[(4-amino-2-methylpyrimidin-5-yl)methyl]-5-[(1r)-1-hydroxyethyl]-3-methyl-2-thienyl}ethyl trihydrogen diphosphate: P29 (= P27), E54 (= E51), V79 (= V76), H118 (= H115), G377 (= G382), T378 (≠ D383), G401 (≠ A406), S402 (= S407), I403 (≠ L408), G427 (= G432), G429 (= G434), S430 (≠ A435), N455 (= N460), G457 (= G462), Y458 (= Y463), T459 (≠ G464), V460 (≠ T465), E461 (= E466)
2vbfB The holostructure of the branched-chain keto acid decarboxylase (kdca) from lactococcus lactis (see paper)
30% identity, 99% coverage: 6:543/544 of query aligns to 8:544/546 of 2vbfB