SitesBLAST
Comparing WP_009545125.1 NCBI__GCF_000017845.1:WP_009545125.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5nofA Anthranilate phosphoribosyltransferase from thermococcus kodakaraensis (see paper)
25% identity, 87% coverage: 44:347/348 of query aligns to 33:320/325 of 5nofA
8bftA The e. Coli trpd2 protein ybib in complex with a c-terminal peptide from obge (see paper)
25% identity, 61% coverage: 2:213/348 of query aligns to 1:206/321 of 8bftA
Sites not aligning to the query:
1kgzB Crystal structure analysis of the anthranilate phosphoribosyltransferase from erwinia carotovora (current name, pectobacterium carotovorum) (see paper)
23% identity, 76% coverage: 20:283/348 of query aligns to 13:254/330 of 1kgzB
- binding manganese (ii) ion: S92 (≠ T103), D222 (= D242), E223 (≠ L243), E223 (≠ L243)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: V79 (≠ L89), G82 (≠ P92), G83 (≠ Y93), N90 (≠ P101), I91 (≠ V102), S92 (≠ T103), T93 (= T104), K108 (≠ L119), S118 (≠ Y129)
3uu1A Anthranilate phosphoribosyltransferase (trpd) from mycobacterium tuberculosis (complex with inhibitor acs142) (see paper)
33% identity, 47% coverage: 20:184/348 of query aligns to 14:169/338 of 3uu1A
- active site: V82 (≠ L89)
- binding 2-amino-3,5-dimethylbenzoic acid: N114 (≠ G122), A151 (≠ L166), P152 (= P167), H155 (≠ F170), Y158 (≠ A173), A162 (≠ V177)
- binding magnesium ion: S95 (≠ T103)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: V82 (≠ L89), G83 (= G90), G85 (≠ P92), G86 (≠ Y93), N93 (≠ P101), S95 (≠ T103), T96 (= T104), K111 (≠ L119), R115 (≠ Q123), A116 (= A124), G118 (≠ P126)
Sites not aligning to the query:
5c7sA Prpp complexed with two mn2+ in the active site of mycobacterium tuberculosis anthranilate phosphoribosyltransferase (anprt; trpd)
32% identity, 47% coverage: 20:184/348 of query aligns to 15:174/344 of 5c7sA
- active site: V83 (≠ L89)
- binding manganese (ii) ion: G84 (= G90), S96 (≠ T103)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: V83 (≠ L89), G84 (= G90), G86 (≠ P92), G87 (≠ Y93), N94 (≠ P101), S96 (≠ T103), T97 (= T104), K112 (≠ L119), N115 (≠ G122), S120 (≠ T127)
Sites not aligning to the query:
4giuA Bianthranilate-like analogue bound in inner site of anthranilate phosphoribosyltransferase (anprt; trpd). (see paper)
32% identity, 47% coverage: 20:184/348 of query aligns to 15:174/346 of 4giuA
- active site: V83 (≠ L89)
- binding 2-[(2-carboxy-5-methylphenyl)amino]-3-methylbenzoic acid: G114 (= G121), N115 (≠ G122), A156 (≠ L166), H160 (≠ F170), Y163 (≠ A173), R170 (= R180)
- binding magnesium ion: S96 (≠ T103)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G84 (= G90), G86 (≠ P92), G87 (≠ Y93), N94 (≠ P101), S96 (≠ T103), T97 (= T104), K112 (≠ L119), N115 (≠ G122), A117 (= A124), A118 (≠ M125), S119 (≠ P126), S120 (≠ T127), G123 (= G130)
Sites not aligning to the query:
4owmA Anthranilate phosphoribosyl transferase from mycobacterium tuberculosis in complex with 3-fluoroanthranilate, prpp and magnesium (see paper)
32% identity, 47% coverage: 20:184/348 of query aligns to 14:173/346 of 4owmA
- active site: V82 (≠ L89)
- binding 2-azanyl-3-fluoranyl-benzoic acid: P156 (= P167), H159 (≠ F170), Y162 (≠ A173), R163 (≠ H174), A166 (≠ V177), R170 (≠ E181)
- binding magnesium ion: S95 (≠ T103)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G83 (= G90), G86 (≠ Y93), N93 (≠ P101), S95 (≠ T103), T96 (= T104), K111 (≠ L119), A117 (≠ M125), S118 (≠ P126), S119 (≠ T127), G122 (= G130)
Sites not aligning to the query:
4gkmA Bianthranilate-like analogue bound in the outer site of anthranilate phosphoribosyltransferase (anprt; trpd) (see paper)
32% identity, 47% coverage: 20:184/348 of query aligns to 14:173/346 of 4gkmA
- active site: V82 (≠ L89)
- binding 2-[(2-carboxyphenyl)amino]-5-methylbenzoic acid: N114 (≠ G122), A155 (≠ L166), P156 (= P167), Y162 (≠ A173), R163 (≠ H174), A166 (≠ V177), R169 (= R180), R170 (≠ E181)
- binding magnesium ion: S95 (≠ T103)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G86 (≠ Y93), N93 (≠ P101), S95 (≠ T103), T96 (= T104), K111 (≠ L119), A117 (≠ M125), S118 (≠ P126), S119 (≠ T127), G122 (= G130)
Sites not aligning to the query:
3r6cA Anthranilate phosphoribosyltransferase (trpd) from mycobacterium tuberculosis (complex with inhibitor acs179) (see paper)
32% identity, 47% coverage: 20:184/348 of query aligns to 14:173/346 of 3r6cA
- active site: V82 (≠ L89)
- binding 8-methoxyphenanthro[3,4-d][1,3]dioxole-5,6-dicarboxylic acid: M62 (≠ L65), N114 (≠ G122), A155 (≠ L166), P156 (= P167), H159 (≠ F170), Y162 (≠ A173), A166 (≠ V177), R169 (= R180)
- binding magnesium ion: S95 (≠ T103)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G83 (= G90), G85 (≠ P92), G86 (≠ Y93), N93 (≠ P101), S95 (≠ T103), T96 (= T104), K111 (≠ L119), N114 (≠ G122), R115 (≠ Q123), A116 (= A124), A117 (≠ M125), S118 (≠ P126), S119 (≠ T127), G122 (= G130), G123 (≠ V131)
Sites not aligning to the query:
- binding 8-methoxyphenanthro[3,4-d][1,3]dioxole-5,6-dicarboxylic acid: 182, 185, 239, 241, 246, 301, 310, 312
- binding magnesium ion: 227, 228, 228
3qsaA Anthranilate phosphoribosyltransferase (trpd) from mycobacterium tuberculosis (complex with inhibitor tamu-a7)
32% identity, 47% coverage: 20:184/348 of query aligns to 14:173/346 of 3qsaA
- active site: V82 (≠ L89)
- binding magnesium ion: S95 (≠ T103)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G83 (= G90), G86 (≠ Y93), N93 (≠ P101), S95 (≠ T103), T96 (= T104), K111 (≠ L119), A117 (≠ M125), S118 (≠ P126), S119 (≠ T127), G122 (= G130)
- binding 4,4,4-trifluoro-1-(4-methoxyphenyl)butane-1,3-dione: M62 (≠ L65), N114 (≠ G122), A155 (≠ L166), P156 (= P167), H159 (≠ F170), Y162 (≠ A173), R163 (≠ H174), A166 (≠ V177)
Sites not aligning to the query:
3qs8A Anthranilate phosphoribosyltransferase (trpd) from mycobacterium tuberculosis (complex with inhibitor acs174) (see paper)
32% identity, 47% coverage: 20:184/348 of query aligns to 14:173/346 of 3qs8A
- active site: V82 (≠ L89)
- binding 2-benzylbenzoic acid: M62 (≠ L65), G83 (= G90), N114 (≠ G122), N114 (≠ G122), A155 (≠ L166), A155 (≠ L166), P156 (= P167), Y162 (≠ A173), Y162 (≠ A173), A166 (≠ V177), R169 (= R180), R169 (= R180), R170 (≠ E181)
- binding magnesium ion: S95 (≠ T103)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G83 (= G90), G85 (≠ P92), G86 (≠ Y93), N93 (≠ P101), S95 (≠ T103), T96 (= T104), K111 (≠ L119), N114 (≠ G122), A116 (= A124), A117 (≠ M125), S118 (≠ P126), S119 (≠ T127), G122 (= G130)
Sites not aligning to the query:
3qqsA Anthranilate phosphoribosyltransferase (trpd) from mycobacterium tuberculosis (complex with inhibitor acs172) (see paper)
32% identity, 47% coverage: 20:184/348 of query aligns to 14:173/346 of 3qqsA
- active site: V82 (≠ L89)
- binding 2,2'-iminodibenzoic acid: P156 (= P167), H159 (≠ F170), Y162 (≠ A173), R163 (≠ H174), A166 (≠ V177), R169 (= R180), R170 (≠ E181)
- binding magnesium ion: S95 (≠ T103)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: V82 (≠ L89), G85 (≠ P92), G86 (≠ Y93), N93 (≠ P101), S95 (≠ T103), T96 (= T104), K111 (≠ L119), R115 (≠ Q123), A117 (≠ M125), S118 (≠ P126), S119 (≠ T127), G122 (= G130)
Sites not aligning to the query:
1zvwA The crystal structure of trpd (rv2192c) from mycobacterium tuberculosis in complex with prpp and magnesium (see paper)
32% identity, 47% coverage: 20:184/348 of query aligns to 14:173/346 of 1zvwA
Sites not aligning to the query:
5c1rA Stereoisomer of prpp bound in the active site of mycobacterium tuberculosis anthranilate phosphoribosyl (anprt; trpd)
32% identity, 47% coverage: 20:184/348 of query aligns to 14:173/343 of 5c1rA
- active site: V82 (≠ L89)
- binding 5-O-[(R)-hydroxy(phosphonooxy)phosphoryl]-1-O-phosphono-alpha-D-ribofuranose: V82 (≠ L89), G83 (= G90), G86 (≠ Y93), N93 (≠ P101), S95 (≠ T103), T96 (= T104), K111 (≠ L119), R115 (≠ Q123), A117 (≠ M125), S118 (≠ P126), S119 (≠ T127)
- binding magnesium ion: D87 (= D94), V89 (≠ R96), S95 (≠ T103)
Sites not aligning to the query:
4owoA Anthranilate phosphoribosyl transferase from mycobacterium tuberculosis in complex with 6-fluoroanthranilate, prpp and magnesium (see paper)
32% identity, 47% coverage: 20:184/348 of query aligns to 16:175/348 of 4owoA
- active site: V84 (≠ L89)
- binding 2-azanyl-6-fluoranyl-benzoic acid: V84 (≠ L89), G85 (= G90), T86 (= T91), H114 (= H120), N116 (≠ G122), N116 (≠ G122), A157 (≠ L166), P158 (= P167), H161 (≠ F170), Y164 (≠ A173), A168 (≠ V177), R171 (= R180)
- binding magnesium ion: S97 (≠ T103)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: V84 (≠ L89), G85 (= G90), G87 (≠ P92), G88 (≠ Y93), N95 (≠ P101), S97 (≠ T103), T98 (= T104), K113 (≠ L119), A118 (= A124), A119 (≠ M125), S120 (≠ P126), S121 (≠ T127), G124 (= G130)
Sites not aligning to the query:
3r88B Anthranilate phosphoribosyltransferase (trpd) from mycobacterium tuberculosis (complex with inhibitor acs145) (see paper)
32% identity, 47% coverage: 20:184/348 of query aligns to 14:173/344 of 3r88B
- active site: V82 (≠ L89)
- binding 2-amino-4,5-dimethoxybenzoic acid: N114 (≠ G122), A155 (≠ L166), P156 (= P167), H159 (≠ F170), Y162 (≠ A173), R163 (≠ H174), A166 (≠ V177)
- binding magnesium ion: S95 (≠ T103)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: V82 (≠ L89), G83 (= G90), G85 (≠ P92), G86 (≠ Y93), N93 (≠ P101), S95 (≠ T103), T96 (= T104), K111 (≠ L119), N114 (≠ G122), A117 (≠ M125), S118 (≠ P126), S119 (≠ T127)
Sites not aligning to the query:
5c2lA Magnesium soaked into the active site of mycobacterium tuberculosis anthranilate phosphoribosyltransferase (anprt; trpd)
32% identity, 47% coverage: 20:184/348 of query aligns to 16:175/349 of 5c2lA
Sites not aligning to the query:
4owuA Anthranilate phosphoribosyl transferase from mycobacterium tuberculosis in complex with 5-methylanthranilate, prpp and magnesium (see paper)
32% identity, 47% coverage: 20:184/348 of query aligns to 15:174/347 of 4owuA
- active site: V83 (≠ L89)
- binding 2-azanyl-5-methyl-benzoic acid: N115 (≠ G122), P157 (= P167), Y163 (≠ A173), R164 (≠ H174), A167 (≠ V177)
- binding magnesium ion: S96 (≠ T103)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G84 (= G90), G86 (≠ P92), G87 (≠ Y93), N94 (≠ P101), S96 (≠ T103), T97 (= T104), K112 (≠ L119), A118 (≠ M125), S120 (≠ T127), G123 (= G130)
Sites not aligning to the query:
4owqA Anthranilate phosphoribosyl transferase from mycobacterium tuberculosis in complex with 3-methylanthranilate, prpp and magnesium (see paper)
32% identity, 47% coverage: 20:184/348 of query aligns to 15:174/347 of 4owqA
- active site: V83 (≠ L89)
- binding 2-azanyl-3-methyl-benzoic acid: P157 (= P167), H160 (≠ F170), Y163 (≠ A173), A167 (≠ V177), R171 (≠ E181)
- binding magnesium ion: S96 (≠ T103)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: V83 (≠ L89), G84 (= G90), G86 (≠ P92), G87 (≠ Y93), N94 (≠ P101), S96 (≠ T103), T97 (= T104), K112 (≠ L119), A117 (= A124), A118 (≠ M125), S120 (≠ T127), G123 (= G130)
Sites not aligning to the query:
4n93A Alternative substrates of mycobacterium tuberculosis anthranilate phosphoribosyl transferase (see paper)
32% identity, 47% coverage: 20:184/348 of query aligns to 15:174/346 of 4n93A
- active site: V83 (≠ L89)
- binding 2-amino-6-methylbenzoic acid: V83 (≠ L89), G84 (= G90), T85 (= T91), H113 (= H120), N115 (≠ G122), N115 (≠ G122), A156 (≠ L166), P157 (= P167), H160 (≠ F170), Y163 (≠ A173), A167 (≠ V177), R170 (= R180)
- binding magnesium ion: S96 (≠ T103)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G84 (= G90), G86 (≠ P92), G87 (≠ Y93), N94 (≠ P101), S96 (≠ T103), T97 (= T104), K112 (≠ L119), A117 (= A124), A118 (≠ M125), S120 (≠ T127), G123 (= G130)
Sites not aligning to the query:
Query Sequence
>WP_009545125.1 NCBI__GCF_000017845.1:WP_009545125.1
MSNTFREYLKKIGSGVHTGRDLTRSESADAMKLMLLAEATPAQIGAFLMVHRIKRPTPEE
LAGMLDTYAELGPKLAIDRLAFDYPVTVLGTPYDGRSRTAPVTTLTTLILATVGVPVVLH
GGQAMPTKYGVPLITLWQGLGVDFSRLSLEESQTMLEKTGLTFVYLPNHFPEAHHLVPYR
EQIGKRPPLATLELMWAPCSSSDVHIVSGFVHPPTENLFRETLKLRNFHYFTTVKGLEGS
CDLPQSRTAIIGINQPKHDPEWQRLHLHSQDYNLSSKDVPLESTEQLLKQMQQVLHGEDN
PLMPLAIYNSGFYLWRCGVCDNLNVAFDQAKTLLTSGRVKQKLQDISK
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory