SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_009545287.1 NCBI__GCF_000017845.1:WP_009545287.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6c6gA An unexpected vestigial protein complex reveals the evolutionary origins of an s-triazine catabolic enzyme. Inhibitor bound complex. (see paper)
47% identity, 98% coverage: 6:452/456 of query aligns to 1:449/457 of 6c6gA

query
sites
6c6gA

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binding calcium ion: Y125 (= Y130), N172 (= N177), F202 (= F207)

2f2aA Structure of tRNA-dependent amidotransferase gatcab complexed with gln (see paper)
35% identity, 97% coverage: 15:456/456 of query aligns to 15:478/485 of 2f2aA

query
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2f2aA

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active site: K79 (= K79), S154 (= S155), S155 (= S156), S173 (= S174), T175 (= T176), G176 (≠ N177), G177 (= G178), S178 (= S179), Q181 (≠ V182)
binding glutamine: G130 (= G131), S154 (= S155), D174 (= D175), T175 (= T176), G176 (≠ N177), S178 (= S179), F206 (= F207), Y309 (≠ A306), Y310 (= Y307), R358 (= R336), D425 (≠ N401)

2dqnA Structure of tRNA-dependent amidotransferase gatcab complexed with asn (see paper)
35% identity, 97% coverage: 15:456/456 of query aligns to 15:478/485 of 2dqnA

query
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2dqnA

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F

Q

E

K

N

V

Y

D

D

V

V

I

V

A

G

P

P

T

T

T

A

P

P

C

T

V

T

A

A

T

F

P

N

L

L

G

G

Q

E

E

E

T

-

M

-

N

-

I

I

N

D

G

D

E

P

E

L

I

T

L

M

I

Y

R

A

P

N

N
x
D

L

L

G

-

R

-

F

L

T

T

Q

T

P

P

L

V

S

N

F

L

I

A

G

G

L

L

P

P

V

G

L

I

S

S

F

V

P

P

I

C

L

G

R

Q

P

S

N

N

K

G

L

R

P

P

L

I

G

G

V

L

Q

Q

L

F

I

I

A

G

A

K

P

P

Y

F

Q

D

E

E

L

K

K

T

I

L

L

Y

Q

R

V

V

A

A

A

Y

F

Q

L

Y

E

E

E

T

K

Q

L

Y

N

N

I

L

active site: K79 (= K79), S154 (= S155), S155 (= S156), S173 (= S174), T175 (= T176), G176 (≠ N177), G177 (= G178), S178 (= S179), Q181 (≠ V182)
binding asparagine: M129 (≠ Y130), G130 (= G131), T175 (= T176), G176 (≠ N177), S178 (= S179), Y309 (≠ A306), Y310 (= Y307), R358 (= R336), D425 (≠ N401)

3h0mA Structure of tRNA-dependent amidotransferase gatcab from aquifex aeolicus (see paper)
34% identity, 98% coverage: 11:455/456 of query aligns to 10:470/478 of 3h0mA

query
sites
3h0mA

L

L

S

R

Q

E

K

L

I

L

K

K

N

R

K

G

E

E

I

V

S

S

A

P

S

K

E

E

V

V

I

V

T

E

Q

S

F

F

T

Y

D

D

N

R

I

Y

E

N

Q

Q

Q

T

N

E

L

E

T

K

L

V

N

K

C

A

F

Y

T

I

A

T

I

P

L

L

K

Y

N

G

Q

K

A

A

V

L

E

K

E

Q

A

A

K

E

N

S

I

L

D

K

Q

E

K

R

I

E

A

L

K

P

G

-

E

-

T

-

I

-

G

-

I

-

L

L

A

F

G

G

V

I

P

P

F

I

A

A

V

V

K
|
K

N

D

L

N

F

I

D

L

I

V

E

E

G

G

I

E

I

K

T

T

L

T

A

C

G

A

S

S

K

K

I

I

N

L

Q

E

K

-

N

N

T

F

P

V

A

A

A

P

E

Y

N

D

A

A

T

T

A

V

I

I

K

E

K

R

L

L

N

K

A

K

A

A

G

G

A

A

I

L

L

I

V

V

G

G

A

K

L

T

N

N

M

L

D

D

E

E

Y

F

A

A

Y
x
M

G
|
G

F

S

V

S

T

T

E

E

N

Y

S

S

H

A

Y

F

G

F

A

P

T

T

P

K

N

N

P

P

H

W

D

D

L

L

S

E

R

R

I

V

S

P

G

G

S
|
S

G

G

S

S

A

A

A

S

V

V

A

A

A

V

N

L

L

S

V

A

P

P

F

V

T

S

L

L

G

G

S
|
S

D
|
D

T
|
T

N
x
G

G
|
G

S
|
S

V

I

R

R

V
x
Q

P

P

A

A

A

S

L

F

C

C

G

G

V

V

L

I

G

G

L

I

K

K

P

P

T

T

Y

Y

G

G

R

R

L

V

S

S

R

R

A

Y

G

G

T

L

V

V

L

A

F
|
F

S

A

N

S

S

S

L

L

D

D

H

Q

I

I

G

G

G

V

F

F

T

G

R

R

S

R

V

T

R

E

D

D

M

V

A

A

A

L

I

V

F

L

D

E

V

V

L

I

Q

S

G

G

E

W

D

D

K

E

T

K

D

D

P

S

V

T

C

S

T

A

S

K

L

V

A

P

P

V

Q

P

L

E

C

W

F

S

D

E

K

E

L

V

N

K

G

K

D

E

I

V

S

K

R

G

L

L

K

K

I

I

A

G

V

L

A

P

G

K

E

E

Y

F

F

F

Q

E

K

Y

G

E

A

L

E

Q

T

P

E

Q

A

V

L

K

K

E

A

A

V

F

E

E

T

N

V

F

A

I

K

K

I

E

L

L

-

E

-

K

-

E

-

G

-

F

N

E

I

I

K

K

E

E

T

-

I

V

N

S

I

L

P

P

E

H

A

V

H

K

R

Y

A

S

R

I

A

P

A

T

A
x
Y

Y

Y

I

I

T

A

A

P

C

S

E

E

G

A

S

S

N

S

-

N

-

L

-

A

-

R

-

Y

-

D

-

G

-

V

-

R

-

Y

-

G

F

Y

H

R

I

A

K

K

N

E

L

Y

-

K

-

D

-

I

-

F

-

E

-

M

-

Y

-

A

R

R

L

T

R

R

P

D

Q

E

D

G

F

F

D

G

T

P

A

E

T

V

R

K

D

R

R
|
R

F

I

L

M

A

L

G

G

A

T

F

F

-

A

-

L

-

S

-

A

-

G

I

Y

P

Y

N

D

T

A

W

Y

Y

Y

I

L

Q

K

A

A

Q

Q

R

K

F

V

R

R

W

L

Y

I

R

T

D

N

Q

D

V

F

K

L

Q

K

I

A

F

F

Q

E

K

E

V

V

D

D

V

V

I

I

I

A

A

S

P

P

T

T

P

P

C

T

V

L

A

P

T

F

P

K

L

F

G

G

Q

E

E

R

T

L

M

E

N

N

I

P

N

I

G

-

E

-

E

E

I

M

L

Y

I

L

R

S

P
x
D

N

I

L

L

G

-

R

-

F

-

T

T

Q

V

P

P

L

A

S

N

F

L

I

A

G

G

L

L

P

P

V

A

L

I

S

S

F

I

P

P

I

I

L

A

R

W

P

K

N

D

K

G

L

L

P

P

L

V

G

G

V

G

Q

Q

L

L

I

I

A

G

A

K

P

H

Y

W

Q

D

E

E

L

T

K

T

I

L

L

L

Q

Q

V

I

A

S

A

Y

F

L

L

W

E

E

E

Q

K

K

L

F

N

K

active site: K72 (= K79), S147 (= S155), S148 (= S156), S166 (= S174), T168 (= T176), G169 (≠ N177), G170 (= G178), S171 (= S179), Q174 (≠ V182)
binding glutamine: M122 (≠ Y130), G123 (= G131), D167 (= D175), T168 (= T176), G169 (≠ N177), G170 (= G178), S171 (= S179), F199 (= F207), Y302 (≠ A306), R351 (= R336), D418 (≠ P400)

3h0lA Structure of tRNA-dependent amidotransferase gatcab from aquifex aeolicus (see paper)
34% identity, 98% coverage: 11:455/456 of query aligns to 10:470/478 of 3h0lA

query
sites
3h0lA

L

L

S

R

Q

E

K

L

I

L

K

K

N

R

K

G

E

E

I

V

S

S

A

P

S

K

E

E

V

V

I

V

T

E

Q

S

F

F

T

Y

D

D

N

R

I

Y

E

N

Q

Q

Q

T

N

E

L

E

T

K

L

V

N

K

C

A

F

Y

T

I

A

T

I

P

L

L

K

Y

N

G

Q

K

A

A

V

L

E

K

E

Q

A

A

K

E

N

S

I

L

D

K

Q

E

K

R

I

E

A

L

K

P

G

-

E

-

T

-

I

-

G

-

I

-

L

L

A

F

G

G

V

I

P

P

F

I

A

A

V

V

K
|
K

N

D

L

N

F

I

D

L

I

V

E

E

G

G

I

E

I

K

T

T

L

T

A

C

G

A

S

S

K

K

I

I

N

L

Q

E

K

-

N

N

T

F

P

V

A

A

A

P

E

Y

N

D

A

A

T

T

A

V

I

I

K

E

K

R

L

L

N

K

A

K

A

A

G

G

A

A

I

L

L

I

V

V

G

G

A

K

L

T

N

N

M

L

D

D

E

E

Y

F

A

A

Y

M

G
|
G

F

S

V

S

T

T

E

E

N

Y

S

S

H

A

Y

F

G

F

A

P

T

T

P

K

N

N

P

P

H

W

D

D

L

L

S

E

R

R

I

V

S

P

G

G

S
|
S

G

G

S

S

A

A

A

S

V

V

A

A

A

V

N

L

L

S

V

A

P

P

F

V

T

S

L

L

G

G

S
|
S

D

D

T
|
T

N
x
G

G
|
G

S
|
S

V

I

R

R

V
x
Q

P

P

A

A

A

S

L

F

C

C

G

G

V

V

L

I

G

G

L

I

K

K

P

P

T

T

Y

Y

G

G

R

R

L

V

S

S

R

R

A

Y

G

G

T

L

V

V

L

A

F

F

S

A

N

S

S

S

L

L

D

D

H

Q

I

I

G

G

G

V

F

F

T

G

R

R

S

R

V

T

R

E

D

D

M

V

A

A

A

L

I

V

F

L

D

E

V

V

L

I

Q

S

G

G

E

W

D

D

K

E

T

K

D

D

P

S

V

T

C

S

T

A

S

K

L

V

A

P

P

V

Q

P

L

E

C

W

F

S

D

E

K

E

L

V

N

K

G

K

D

E

I

V

S

K

R

G

L

L

K

K

I

I

A

G

V

L

A

P

G

K

E

E

Y

F

F

F

Q

E

K

Y

G

E

A

L

E

Q

T

P

E

Q

A

V

L

K

K

E

A

A

V

F

E

E

T

N

V

F

A

I

K

K

I

E

L

L

-

E

-

K

-

E

-

G

-

F

N

E

I

I

K

K

E

E

T

-

I

V

N

S

I

L

P

P

E

H

A

V

H

K

R

Y

A

S

R

I

A

P

A

T

A
x
Y

Y

Y

I

I

T

A

A

P

C

S

E

E

G

A

S

S

N

S

-

N

-

L

-

A

-

R

-

Y

-

D

-

G

-

V

-

R

-

Y

-

G

F

Y

H

R

I

A

K

K

N

E

L

Y

-

K

-

D

-

I

-

F

-

E

-

M

-

Y

-

A

R

R

L

T

R

R

P

D

Q

E

D

G

F

F

D

G

T

P

A

E

T

V

R

K

D

R

R
|
R

F

I

L

M

A

L

G

G

A

T

F

F

-

A

-

L

-

S

-

A

-

G

I

Y

P

Y

N

D

T

A

W

Y

Y

Y

I

L

Q

K

A

A

Q

Q

R

K

F

V

R

R

W

L

Y

I

R

T

D

N

Q

D

V

F

K

L

Q

K

I

A

F

F

Q

E

K

E

V

V

D

D

V

V

I

I

I

A

A

S

P

P

T

T

P

P

C

T

V

L

A

P

T

F

P

K

L

F

G

G

Q

E

E

R

T

L

M

E

N

N

I

P

N

I

G

-

E

-

E

E

I

M

L

Y

I

L

R

S

P
x
D

N

I

L

L

G

-

R

-

F

-

T

T

Q

V

P

P

L

A

S

N

F

L

I

A

G

G

L

L

P

P

V

A

L

I

S

S

F

I

P

P

I

I

L

A

R

W

P

K

N

D

K

G

L

L

P

P

L

V

G

G

V

G

Q

Q

L

L

I

I

A

G

A

K

P

H

Y

W

Q

D

E

E

L

T

K

T

I

L

L

L

Q

Q

V

I

A

S

A

Y

F

L

L

W

E

E

E

Q

K

K

L

F

N

K

active site: K72 (= K79), S147 (= S155), S148 (= S156), S166 (= S174), T168 (= T176), G169 (≠ N177), G170 (= G178), S171 (= S179), Q174 (≠ V182)
binding asparagine: G123 (= G131), S147 (= S155), G169 (≠ N177), G170 (= G178), S171 (= S179), Y302 (≠ A306), R351 (= R336), D418 (≠ P400)

3kfuE Crystal structure of the transamidosome (see paper)
35% identity, 96% coverage: 14:452/456 of query aligns to 8:455/468 of 3kfuE

query
sites
3kfuE

K

R

I

V

K

A

N

R

K

G

E

E

I

V

S

S

A

P

S

L

E

E

V

V

I

A

T

Q

Q

A

F

Y

T

L

D

K

N

R

I

V

E

Q

Q

E

Q

L

N

D

L

P

T

G

L

L

N

G

C

A

F

F

T

L

A

S

I

-

L

L

K

N

N

E

Q

R

A

L

V

L

E

E

A

A

K

E

N

A

I

V

D

D

Q

P

K

G

I

L

A

P

K

-

G

-

E

-

T

-

I

-

G

-

I

-

L

L

A

A

G

G

V

L

P

V

F

V

A

A

V

V

K
|
K

N

D

L

N

F

I

D

A

I

T

E

R

G

G

I

L

I

R

T

T

L

T

A

A

G

G

S

S

K

R

I

L

N

L

Q

E

K

N

N

F

T

V

P

P

A

P

A

Y

E

E

N

-

A

A

T

T

A

A

I

V

K

A

K

R

L

L

N

K

A

A

A

L

G

G

A

A

I

L

L

V

V

L

G

G

A

K

L

T

N

N

M

L

D

D

E

E

Y

F

A

G

Y

M

G

G

F

S

V

S

T

T

E

E

N

H

S

S

H

A

Y

F

G

F

A

P

T

T

P

K

N

N

P

P

H

F

D

D

L

P

S

D

R

R

I

V

S

P

G

G

S
|
S

G

G

S

S

A

A

V

L

A

A

N

D

L

L

V

A

P

P

F

L

T

A

L

L

G

G

S
|
S

D

D

T
|
T

N
x
G

G
|
G

S
|
S

V

V

R

R

V
x
Q

P

P

A

A

L

F

C

C

G

G

V

V

L

Y

G

G

L

L

K

K

P

P

T

T

Y

Y

G

G

R

R

L

V

S

S

R

R

A

F

G

G

T

L

V

I

L

A

F

Y

S

A

N

S

S

S

L

L

D

D

H

Q

I

I

G

G

G

P

F

M

T

A

R

R

S

S

V

V

R

R

D

D

M

L

A

A

A

L

I

L

F

M

D

D

V

A

L

A

Q

A

G

G

E

P

D

D

K

P

T

L

D

D

P

A

V

T

C

S

T

L

S

D

L

L

A

P

P

P

Q

R

L

F

C

Q

F

-

D

E

K

A

L

L

N

E

G

G

D

P

I

L

S

P

R

P

L

L

K

R

I

L

A

G

V

V

A

V

G

R

E

E

Y

A

F

L

-

A

-

G

-

N

Q

S

K

P

G

G

A

V

E

E

-

R

-

A

-

L

T

E

E

E

A

A

L

L

K

K

A

V

V

F

E

R

T

E

V

L

A

G

K

-

I

-

L

L

N

S

I

V

K

R

E

E

T

-

I

V

N

S

I

W

P

P

E

S

A

L

H

P

R

Q

A

A

R

L

A

A

A

Y

Y

Y

I

I

I

L

T

A

A

P

C

A

E

E

G

A

S

S

-

S

-

N

-

L

-

A

-

R

-

Y

N

D

F

G

H

T

I

L

K

Y

N

G

L

R

R

R

L

A

R

A

P

G

Q

E

D

E

-

V

-

E

-

G

-

M

-

E

-

A

-

T

-

R

-

A

-

L

F

F

D

G

T

L

A

E

T

V

R

K

D

R

R

R

F

V

L

L

A

V

G

G

A

T

F

F

I

V

P

L

N

S

T

S

-
x
G

-

Y

-
x
Y

-

E

-

A

W

Y

Y

Y

I

G

Q

R

A

A

Q

Q

R

A

F

F

R

R

W

R

Y

L

R

K

D

A

Q

E

V

A

K

Q

Q

A

I

L

F

F

Q

R

K

E

V

V

D

D

V

L

I

L

A

L

P

P

T

T

P

P

C

H

V

P

A

A

T

F

P

P

L

F

G

G

-

A

-

R

Q

R

E

D

T

P

M

L

N

A

I

M

N

Y

G

R

E

E

E

D

I

L

L

-

I

-

R

-

P

-

N

-

L

-

G

-

R

-

F

Y

T

T

Q

V

P

G

L

A

S

N

F

L

I

T

G

G

L

L

P

P

V

A

L

L

S

S

F

F

P

P

I

A

L

G

R

F

P

E

N

G

K

H

L

L

P

P

L

V

G

G

V

L

Q

Q

L

L

I

L

A

A

A

P

P

W

Y

G

Q

E

E

D

L

E

K

R

I

L

L

L

Q

R

V

A

A

A

A

L

F

A

L

F

E

E

active site: K65 (= K79), S140 (= S155), S141 (= S156), S159 (= S174), T161 (= T176), G162 (≠ N177), G163 (= G178), S164 (= S179), Q167 (≠ V182)
binding : G351 (vs. gap), Y353 (vs. gap)

Q7XJJ7 Fatty acid amide hydrolase; AtFAAH; N-acylethanolamine amidohydrolase; EC 3.5.1.99 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
32% identity, 89% coverage: 49:452/456 of query aligns to 175:589/607 of Q7XJJ7

query
sites
Q7XJJ7

N

N

Q

E

A

V

V

I

E

K

E

Q

A

A

K

E

N

A

I

S

D

T

Q

R

K

R

I

F

A

E

K

Q

G

G

E

N

T

P

I

I

G

S

I

V

L

L

A

D

G

G

V

I

P

F

F

V

A

T

V

I

K
|
K

N

D

L

D

F

I

D

D

I

C

E

L

G

P

I

H

I

P

T

T

L

N

A

G

G

G

S

T

K

T

I

W

N

L

Q

H

K

E

N

D

T

R

P

S

A

V

A

E

E

K

N

D

A

S

T

A

A

V

I

V

K

S

K

K

L

L

N

R

A

S

A

C

G

G

A

A

I

I

L

L

V

L

G

G

A

K

L

A

N

N

M

M

D

H

E

E

Y

L

A

G

Y

M

G

G

F

T

V

T

T

G

E

N

N

N

S

S

H

N

Y

Y

G

G

A

T

T

T

P

R

N

N

P

P

H

H

D

D

L

P

S

K

R

R

I

Y

S

T

G

G

S
|
S

G

S

G

G

S

S

A

A

A

I

V

V

A

A

N

G

L

L

V

C

P

S

F

A

T

A

L

L

G

G

S

T

D

D

T
x
G

N
x
G

G
|
G

S
|
S

V

V

R
|
R

V

I

P

P

A

S

A

A

L

L

C

C

G

G

V

I

L

T

G

G

L

L

K

K

P

T

T

T

Y

Y

G

G

R

R

L

T

S

D

R

M

A

T

G

G

T

S

V

L

L

C

F

E

S

G

N

G

S

T

L

V

D

E

H

I

I

I

G

G

G

P

F

L

T

A

R

S

S

S

V

L

R

E

D

D

M

A

A

F

A

L

I

V

F

Y

D

A

V

A

L

I

Q

L

G

G

E
x
S

D

S

K

S

T

A

D

D

P

R

V

Y

C

N

T

L

S

K

L

P

A

S

P

P

Q

P

L

-

C

C

F

F

D

P

K

K

L

L

-

L

-

S

-

H

N

N

G

G

D

S

-

N

-

A

I

I

S

G

R

S

L

L

K

R

I

L

A

G

V

K

A

Y

G

T

E

K

Y

W

F

F

Q

N

K

D

G

V

A

S

E

S

T

S

E

D

A

I

L

S

K

D

A

K

V

C

E

E

T

D

V

I

A

L

K

K

I

L

L

L

N

S

I

N

K

N

E

H

-

G

-

C

-

K

-

V

T

E

I

I

N

V

I

V

P

P

E

E

A

L

H

E

R

E

A

M

R

R

A

A

A

H

Y

V

I

I

I

S

T

I

A

G

-

S

-

P

-

T

-

L

-

S

-

L

-

T

-

P

-

Y

C

C

E

E

G

A

S

G

N

-

F

-

H

-

I

-

K

K

N

N

L

S

R

K

L

L

R

-

P

-

Q

-

D

S

F

Y

D

D

T

-

A

-

T

T

R

R

D

T

R

S

F

F

L

A

A

I

G

F

A

R

F

S

I

F

P

S

N

A

T

S

W

D

Y

Y

I

I

Q

A

A

A

Q

Q

R

C

F

L

R

R

W

R

Y

L

R

M

D

E

Q

Y

V

H

K

L

Q

N

I

I

F

F

Q

K

K

D

V

V

D

D

V

V

I

I

I

V

A

T

P

P

T

T

P

G

C

M

V

T

A

A

T

P

P

V

L

I

G

P

Q

P

E

D

T

A

M

L

N

K

I

-

N

N

G

G

E

E

-

T

E

N

I

I

L

Q

I

V

R

T

P

T

N

D

L

L

G

M

R

R

F

F

T

V

Q

L

P

A

L

A

S

N

F

L

I

L

G

G

L

F

P

P

V

A

L

I

S

S

F

V

P

P

I

V

-

G

L

Y

R

D

P

K

N

E

K

G

L

L

P

P

L

I

G

G

V

L

Q

Q

L

I

I

M

A

G

A

R

P

P

Y

W

Q

A

E

E

L

A

K

T

I

V

L

L

Q

G

V

L

A

A

F

A

L

V

E

E

K205 (= K79) mutation to A: Loss of activity.
SS 281:282 (= SS 155:156) mutation to AA: Loss of activity.
GGGS 302:305 (≠ TNGS 176:179) binding substrate
S305 (= S179) mutation to A: Loss of activity.
R307 (= R181) mutation to A: Loss of activity.
S360 (≠ E234) mutation to A: No effect.

6diiH Structure of arabidopsis fatty acid amide hydrolase in complex with methyl linolenyl fluorophosphonate (see paper)
32% identity, 89% coverage: 49:452/456 of query aligns to 175:589/616 of 6diiH

query
sites
6diiH

N

N

Q

E

A

V

V

I

E

K

E

Q

A

A

K

E

N

A

I

S

D

T

Q

R

K

R

I

F

A

E

K

Q

G

G

E

N

T

P

I

I

G

S

I

V

L

L

A

D

G

G

V

I

P

F

F

V

A

T

V

I

K

K

N

D

L

D

F

I

D

D

I

C

E

L

G

P

I

H

I

P

T

T

L

N

A

G

G

G

S

T

K

T

I

W

N

L

Q

H

K

E

N

D

T

R

P

S

A

V

A

E

E

K

N

D

A

S

T

A

A

V

I

V

K

S

K

K

L

L

N

R

A

S

A

C

G

G

A

A

I

I

L

L

V

L

G

G

A

K

L

A

N

N

M

M

D

H

E

E

Y

L

A
x
G

Y

M

G

G

F
x
T

V

T

T

G

E

N

N

N

S

S

H

N

Y

Y

G

G

A

T

T

T

P

R

N

N

P

P

H

H

D

D

L

P

S

K

R

R

I

Y

S

T

G

G

S
|
S

S

S

G

S

G

G

S

S

A

A

A

I

V

V

A

A

N

G

L

L

V

C

P

S

F

A

T

A

L

L

G

G

S

T

D

D

T
x
G

N
x
G

G

G

S
|
S

V

V

R

R

V

I

P

P

A

S

A

A

L

L

C

C

G

G

V

I

L

T

G

G

L

L

K

K

P

T

T

T

Y

Y

G

G

R

R

L

T

S

D

R

M

A

T

G

G

T

S

V

L

L

C

F

E

S

G

N

G

S

T

L

V

D

E

H

I

I

I

G

G

G

P

F

L

T

A

R

S

S

S

V

L

R

E

D

D

M

A

A

F

A

L

I

V

F

Y

D

A

V

A

L

I

Q

L

G

G

E

S

D

S

K

S

T

A

D

D

P

R

V

Y

C

N

T

L

S

K

L

P

A

S

P

P

Q

P

L

-

C

C

F

F

D

P

K

K

L

L

-

L

-

S

-

H

N

N

G

G

D

S

-

N

-

A

I

I

S

G

R

S

L

L

K

R

I

L

A

G

V

K

A

Y

G

T

E

K

Y

W

F

F

Q

N

K

D

G

V

A

S

E

S

T

S

E

D

A

I

L

S

K

D

A

K

V

C

E

E

T

D

V

I

A

L

K

K

I

L

L

L

N

S

I

N

K

N

E

H

-

G

-

C

-

K

-

V

T

E

I

I

N

V

I

V

P

P

E

E

A

L

H

E

R

E

A

M

R

R

A

A

A

H

Y

V

I

I

I

S

T

I

A

G

-

S

-

P

-

T

-

L

-

S

-

L

-

T

-

P

-

Y

C

C

E

E

G

A

S

G

N

-

F

-

H

-

I

-

K

K

N

N

L

S

R

K

L

L

R

-

P

-

Q

-

D

S

F

Y

D

D

T

-

A

-

T

T

R

R

D

T

R
x
S

F

F

L

A

A

I

G

F

A

R

F

S

I

F

P

S

N

A

T

S

W

D

Y

Y

I

I

Q

A

A

A

Q

Q

R

C

F

L

R

R

W

R

Y

L

R

M

D

E

Q

Y

V

H

K

L

Q

N

I

I

F

F

Q

K

K

D

V

V

D

D

V

V

I

I

I

V

A

T

P

P

T

T

P

G

C

M

V

T

A

A

T

P

P

V

L

I

G

P

Q

P

E

D

T

A

M

L

N

K

I

-

N

N

G

G

E

E

-

T

E

N

I
|
I

L

Q

I

V

R

T

P

T

N

D

L

L

G
x
M

R

R

F

F

T

V

Q

L

P

A

L

A

S

N

F

L

I

L

G

G

L

F

P

P

V

A

L

I

S

S

F

V

P

P

I

V

-

G

L

Y

R

D

P

K

N

E

K

G

L

L

P

P

L

I

G

G

V

L

Q

Q

L

I

I

M

A

G

A

R

P

P

Y

W

Q

A

E

E

L

A

K

T

I

V

L

L

Q

G

V

L

A

A

F

A

L

V

E

E

binding methyl-9Z,12Z,15Z-octadecatrienylphosphonofluoridate: G255 (≠ A129), T258 (≠ F132), S281 (= S155), G302 (≠ T176), G303 (≠ N177), S305 (= S179), S472 (≠ R336), I532 (= I396), M539 (≠ G403)

Sites not aligning to the query:

binding methyl-9Z,12Z,15Z-octadecatrienylphosphonofluoridate: 25, 61

8ey9B Structure of arabidopsis fatty acid amide hydrolase mutant s305a in complex with 9-hydroxy-10,12-octadecadienoyl-ethanolamide
32% identity, 89% coverage: 49:452/456 of query aligns to 175:589/605 of 8ey9B

query
sites
8ey9B

N

N

Q

E

A

V

V

I

E

K

E

Q

A

A

K

E

N

A

I

S

D

T

Q

R

K

R

I

F

A

E

K

Q

G

G

E

N

T

P

I

I

G

S

I

V

L

L

A

D

G

G

V

I

P

F

F

V

A

T

V

I

K

K

N

D

L

D

F

I

D

D

I

C

E

L

G

P

I

H

I

P

T

T

L

N

A

G

G

G

S

T

K

T

I

W

N

L

Q

H

K

E

N

D

T

R

P

S

A

V

A

E

E

K

N

D

A

S

T

A

A

V

I

V

K

S

K

K

L

L

N

R

A

S

A

C

G

G

A

A

I

I

L

L

V

L

G

G

A

K

L

A

N

N

M

M

D

H

E

E

Y

L

A
x
G

Y

M

G

G

F

T

V

T

T

G

E

N

N

N

S

S

H

N

Y

Y

G

G

A

T

T

T

P

R

N

N

P

P

H

H

D

D

L

P

S

K

R

R

I

Y

S

T

G

G

S

S

G

S

G

G

S

S

A

A

A

I

V

V

A

A

N

G

L

L

V

C

P

S

F

A

T

A

L

L

G

G

S

T

D

D

T
x
G

N
x
G

G
|
G

S
x
A

V

V

R

R

V

I

P

P

A

S

A

A

L

L

C

C

G

G

V

I

L

T

G

G

L

L

K

K

P

T

T

T

Y

Y

G

G

R

R

L

T

S

D

R

M

A

T

G

G

T

S

V

L

L

C

F

E

S

G

N

G

S

T

L

V

D

E

H

I

I

I

G

G

G

P

F

L

T

A

R

S

S

S

V

L

R

E

D

D

M

A

A

F

A

L

I

V

F

Y

D

A

V

A

L

I

Q

L

G

G

E

S

D

S

K

S

T

A

D

D

P

R

V

Y

C

N

T

L

S

K

L

P

A

S

P

P

Q

P

L

-

C

C

F

F

D

P

K

K

L

L

-

L

-

S

-

H

N

N

G

G

D

S

-

N

-

A

I

I

S

G

R

S

L

L

K

R

I

L

A

G

V

K

A

Y

G

T

E

K

Y

W

F

F

Q

N

K

D

G

V

A

S

E

S

T

S

E

D

A

I

L

S

K

D

A

K

V

C

E

E

T

D

V

I

A

L

K

K

I

L

L

L

N

S

I

N

K

N

E

H

-

G

-

C

-

K

-

V

T

E

I

I

N

V

I

V

P

P

E

E

A

L

H

E

R

E

A

M

R

R

A

A

A

H

Y
x
V

I

I

I

S

T

I

A

G

-

S

-

P

-

T

-

L

-

S

-

L

-

T

-

P

-

Y

C

C

E

E

G

A

S

G

N

-

F

-

H

-

I

-

K

K

N

N

L

S

R

K

L

L

R

-

P

-

Q

-

D

S

F

Y

D

D

T

-

A

-

T

T

R

R

D

T

R

S

F

F

L

A

A
x
I

G

F

A

R

F

S

I

F

P

S

N

A

T

S

W

D

Y

Y

I

I

Q

A

A

A

Q

Q

R

C

F

L

R

R

W

R

Y

L

R

M

D

E

Q

Y

V

H

K

L

Q

N

I

I

F

F

Q

K

K

D

V

V

D

D

V

V

I

I

I

V

A

T

P

P

T

T

P

G

C

M

V

T

A

A

T

P

P

V

L

I

G

P

Q

P

E

D

T

A

M

L

N

K

I

-

N

N

G

G

E

E

-

T

E

N

I

I

L

Q

I

V

R

T

P

T

N

D

L

L

G
x
M

R

R

F

F

T

V

Q

L

P

A

L

A

S

N

F

L

I

L

G

G

L

F

P

P

V

A

L

I

S

S

F

V

P

P

I

V

-

G

L

Y

R

D

P

K

N

E

K

G

L

L

P

P

L

I

G

G

V

L

Q

Q

L

I

I

M

A

G

A

R

P

P

Y

W

Q

A

E

E

L

A

K

T

I

V

L

L

Q

G

V

L

A

A

F

A

L

V

E

E

binding (9R,10E,12Z)-9-hydroxy-N-(2-hydroxyethyl)octadeca-10,12-dienamide: G255 (≠ A129), G302 (≠ T176), G303 (≠ N177), G304 (= G178), A305 (≠ S179), V442 (≠ Y307), I475 (≠ A339), M539 (≠ G403)

Sites not aligning to the query:

binding (9R,10E,12Z)-9-hydroxy-N-(2-hydroxyethyl)octadeca-10,12-dienamide: 55

8ey1D Structure of arabidopsis fatty acid amide hydrolase mutant s305a in complex with n-(3-oxododecanoyl)-l-homoserine lactone
32% identity, 89% coverage: 49:452/456 of query aligns to 175:589/605 of 8ey1D

query
sites
8ey1D

N

N

Q

E

A

V

V

I

E

K

E

Q

A

A

K

E

N

A

I

S

D

T

Q

R

K

R

I

F

A

E

K

Q

G

G

E

N

T

P

I

I

G

S

I

V

L

L

A

D

G

G

V

I

P

F

F

V

A

T

V

I

K

K

N

D

L

D

F

I

D

D

I

C

E

L

G

P

I

H

I

P

T

T

L

N

A

G

G

G

S

T

K

T

I

W

N

L

Q

H

K

E

N

D

T

R

P

S

A

V

A

E

E

K

N

D

A

S

T

A

A

V

I

V

K

S

K

K

L

L

N

R

A

S

A

C

G

G

A

A

I

I

L

L

V

L

G

G

A

K

L

A

N

N

M

M

D

H

E

E

Y

L

A
x
G

Y

M

G

G

F

T

V

T

T

G

E

N

N

N

S

S

H

N

Y

Y

G

G

A

T

T

T

P

R

N

N

P

P

H

H

D

D

L

P

S

K

R

R

I

Y

S

T

G

G

S
|
S

S

S

G

S

G

G

S

S

A

A

A

I

V

V

A

A

N

G

L

L

V

C

P

S

F

A

T

A

L

L

G

G

S
x
T

D

D

T
x
G

N
x
G

G

G

S
x
A

V

V

R

R

V

I

P

P

A

S

A

A

L

L

C

C

G

G

V

I

L

T

G

G

L

L

K

K

P

T

T

T

Y

Y

G

G

R

R

L

T

S

D

R

M

A

T

G

G

T

S

V

L

L

C

F

E

S

G

N

G

S

T

L

V

D
x
E

H

I

I

I

G

G

G

P

F

L

T

A

R

S

S

S

V

L

R

E

D

D

M

A

A

F

A

L

I

V

F

Y

D

A

V

A

L

I

Q

L

G

G

E

S

D

S

K

S

T

A

D

D

P

R

V

Y

C

N

T

L

S

K

L

P

A

S

P

P

Q

P

L

-

C

C

F

F

D

P

K

K

L

L

-

L

-

S

-

H

N

N

G

G

D

S

-

N

-

A

I

I

S

G

R

S

L

L

K

R

I

L

A

G

V

K

A

Y

G

T

E

K

Y

W

F

F

Q

N

K

D

G

V

A

S

E

S

T

S

E

D

A

I

L

S

K

D

A

K

V

C

E

E

T

D

V

I

A

L

K

K

I

L

L

L

N

S

I

N

K

N

E

H

-

G

-

C

-

K

-

V

T

E

I

I

N

V

I

V

P

P

E

E

A

L

H

E

R

E

A

M

R

R

A

A

A

H

Y

V

I

I

I

S

T

I

A

G

-

S

-

P

-

T

-

L

-

S

-

L

-

T

-

P

-

Y

C

C

E

E

G

A

S

G

N

-

F

-

H

-

I

-

K

K

N

N

L

S

R

K

L

L

R

-

P

-

Q

-

D

S

F

Y

D

D

T

-

A

-

T

T

R

R

D

T

R

S

F

F

L

A

A

I

G

F

A

R

F

S

I

F

P

S

N

A

T

S

W

D

Y

Y

I

I

Q

A

A

A

Q

Q

R

C

F

L

R

R

W

R

Y

L

R

M

D

E

Q

Y

V

H

K

L

Q

N

I

I

F

F

Q

K

K

D

V

V

D

D

V

V

I

I

I

V

A

T

P

P

T

T

P

G

C

M

V

T

A

A

T

P

P

V

L

I

G

P

Q

P

E

D

T

A

M

L

N

K

I

-

N

N

G

G

E

E

-

T

E

N

I

I

L

Q

I

V

R

T

P

T

N

D

L

L

G
x
M

R

R

F

F

T

V

Q

L

P

A

L

A

S

N

F

L

I

L

G

G

L

F

P

P

V

A

L

I

S

S

F

V

P

P

I

V

-

G

L

Y

R

D

P

K

N

E

K

G

L

L

P

P

L

I

G

G

V

L

Q

Q

L

I

I

M

A

G

A

R

P

P

Y

W

Q

A

E

E

L

A

K

T

I

V

L

L

Q

G

V

L

A

A

F

A

L

V

E

E

binding n-3-oxo-dodecanoyl-l-homoserine lactone: G255 (≠ A129), S281 (= S155), T300 (≠ S174), G302 (≠ T176), G303 (≠ N177), A305 (≠ S179), E338 (≠ D212), M539 (≠ G403)

5h6sC Crystal structure of hydrazidase s179a mutant complexed with a substrate (see paper)
27% identity, 98% coverage: 6:453/456 of query aligns to 5:446/457 of 5h6sC

query
sites
5h6sC

L

L

D

T

A

A

I

T

A

E

L

I

S

R

Q

A

K

R

I

I

K

S

N

E

K

G

E

A

I

A

S

S

A

R

S

E

E

E

V

V

I

V

T

H

Q

E

F

H

T

L

D

D

N

R

I

I

E

D

Q

E

Q

F

N

N

L

A

T

L

L

T

N

N

C

S

F

F

T

V

A

E

I

L

L

R

K

A

N

D

Q

Q

A

V

V

L

E

E

A

A

K

R

N

A

I

A

D

D

Q

R

K

E

I

F

A

-

K

-

G

G

E

S

T

T

I

L

G

G

-

G

I

P

L

L

A

D

G

G

V

V

P

P

F

L

A

S

V

I

K
|
K

N

D

L

S

F

Y

D

S

I

V

E

A

G

G

I

L

I

H

T

R

L

T

A

D

G

G

S

L

K

P

I

V

N

N

Q

-

K

A

N

D

T

V

P

L

A

D

A

A

E

Q

N

D

A

D

T

V

A

A

I

T

K

A

K

R

L

L

N

R

A

A

G

G

A

G

I

L

L

V

V

L

G

G

A

H

L

A

N

G

M

I

D

P

E

D

Y

L

A
x
C

Y

I

G
x
R

F
x
W

V

N

T

S

E

V

N

S

S

G

H

L

Y

Y

G

G

A

A

T

V

P

R

N

N

P

P

H

R

D

D

L

L

S

S

R

R

I

T

S

A

G

G

S
|
S

G

G

S

D

A

A

A

N

V

V

A

A

N

G

L

F

V

A

P

T

F

I

T

G

L

L

G

G

S

G

D

D

T
x
L

N
x
G

G
|
G

S
|
S

V

I

R

R

V

V

P

P

A

A

A

S

L

W

C

C

G

G

V

V

L

Y

G

G

L

F

K

R

P

T

T

G

Y

P

G

G

R

R

L

I

-

P

-

D

-

V

-

N

-

P

-

N

-

G

S

G

R

R

A

S

G

R

T

N

V

V

L

V

F

M

S

-

N

E

S

L

L

M

D

A

H

Q

I

I

G

G

G

P

F

I

T

A

R

R

S

S

V

I

R

D

D

D

M

I

A

E

A

L

I

A

F

F

D

R

V

I

L

M

Q

T

G

G

E

V

D

D

K

R

T

R

D

D

P

T

V

M

C

S

T

S

S

P

L

L

A

G

P

-

Q

-

L

-

C

-

F

-

D

-

K

-

L

L

N

I

G

E

D

P

I

I

S

E

R

A

L

P

K

R

I

V

A

A

V

V

-

L

-

R

-

H

-

E

A

T

G

G

E

A

Y

V

F

L

Q

D

K

S

G

S

A

V

E

E

T

E

E

Q

A

L

L

D

K

A

A

T

V

I

E

E

T

M

V

L

A

R

K

A

I

E

L

G

N

Y

I

V

K

V

E

E

T

E

I

N

N

V

I

L

P

P

E

D

A

L

H

H

R

R

A

A

R

P

A

E

A

V

A
x
W

Y

A

I

E

I

I

T

V

A

G

C

T

-

E

-

L

-

I

-

H

-

R

-

V

-

L

-

P

E

E

G

V

S

A

N

E

F

L

H

V

I

I

K

A

N

S

L

E

R

R

L

M

R

H

P

I

Q

V

D

D

F

M

D
x
F

T

G

A

A

T

Y

R

E

D

L

R

G

F

A

L

D

A

V

G

G

A

A

F

-

I

-

P

-

N

-

T

-

W

-

Y

Y

I

L

Q

T

A

A

Q

L

R

E

F

E

R

R

R

S

W

S

Y

I

R

Q

D

M

Q

T

V

V

K

A

Q

A

I

L

F

M

Q

E

K

R

V

Y

D

Q

V

L

I

I

I

L

A

A

P

P

T

V

T

A

P

G

C

M

V

P

A

A

T

P

P

P

L

L

G

D

Q

F

E

D

T

D

M

-

N

H

I

I

N

G

G

R

E

E

E

A

I

S

L

I

I

A

R

L

P

F

N

D

L

Q

G

M

R

R

F

C

T

V

Q

P

P

W

L

V

S

N

F

L

I

L

G

G

L

L

P

P

V

S

L

L

S

A

F

-

P

-

I

-

L

-

R

-

P

-

N

-

K

-

L

L

P

P

L

N

G

G

V

I

Q

Q

L

L

I

V

A

G

A

R

P

K

Y

H

Q

D

E

E

L

L

K

T

I

I

L

L

Q

A

V

A

A

G

A

R

F

A

L

Y

E

E

E

R

K

R

active site: K77 (= K79), S152 (= S155), S153 (= S156), L173 (≠ T176), G174 (≠ N177), G175 (= G178), S176 (= S179)
binding 4-oxidanylbenzohydrazide: C126 (≠ A129), R128 (≠ G131), W129 (≠ F132), S152 (= S155), L173 (≠ T176), G174 (≠ N177), S176 (= S179), W306 (≠ A306), F338 (≠ D330)

Q9FR37 Amidase 1; AtAMI1; Translocon at the outer membrane of chloroplasts 64-I; AtTOC64-I; EC 3.5.1.4 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
41% identity, 37% coverage: 71:240/456 of query aligns to 28:198/425 of Q9FR37

query
sites
Q9FR37

L

L

A

Q

G

G

V

L

P

T

F

F

A

A

V

I

K
|
K

N

D

L

I

F

F

D

D

I

V

E

E

G

G

I

R

I

V

T

T

L

G

A

F

G

G

S

N

-

P

K

D

I

W

N

L

Q

R

K

T

N

H

T

S

P

A

A

A

A

T

E

S

N

T

A

A

T

P

A

V

I

V

K

S

L

L

N

L

A

E

A

A

G

G

A

A

I

T

L

A

V

L

G

G

A

I

L

T

N

I

M

M

D

D

E

E

Y

M

A

A

Y

Y

G

S

F

I

V

N

T

G

E

E

N

N

S

A

H

H

Y

Y

G

G

A

T

T

P

P

R

N

N

P

P

H

I

D

A

L

F

S

D

R

R

I

V

S

P

G

G

S
|
S

G

S

G

G

S

S

A

A

A

V

A

A

V

V

A

A

N

R

L

L

V

V

P

D

F

F

T

S

L

I

G

G

S

T

D
|
D

T

T

N

G

G

G

S
|
S

V

V

R

R

V

V

P

P

A

A

A

S

L

Y

C
|
C

G

G

V

I

L

F

G

G

L

F

K

R

P

P

T

S

Y

H

G

G

R

A

L

V

S

S

R

T

A

V

G

G

T

L

V

T

L

P

F

M

S

A

N

Q

S

S

L

F

D

D

H

T

I

V

G

G

G

W

F

F

T

A

R

R

S

D

V

T

R

A

D

T

M

L

A

K

A

R

I

V

F

G

D

C

V

V

L

L

Q

L

G

Q

E

Q

D

H

K

H

T

L

D

N

P

P

V

I

K36 (= K79) active site, Charge relay system; mutation to A: Loss of catalytic activity.; mutation to R: Reduces catalytic activity 10-fold.
S113 (= S155) active site, Charge relay system; mutation S->A,T: Loss of catalytic activity.
S114 (= S156) mutation to A: Loss of catalytic activity.; mutation to T: Reduces catalytic activity 400-fold.
D133 (= D175) mutation to A: Loss of catalytic activity.; mutation to E: Reduces catalytic activity 600-fold.
S137 (= S179) active site, Acyl-ester intermediate; mutation to A: Reduces catalytic activity 170-fold.; mutation to T: Loss of catalytic activity.
C145 (= C187) mutation C->A,S: Reduces catalytic activity 10-fold.

Sites not aligning to the query:

214 S→T: Slightly reduces catalytic activity.

Q84DC4 Mandelamide hydrolase; EC 3.5.1.86 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
28% identity, 97% coverage: 12:455/456 of query aligns to 34:491/507 of Q84DC4

query
sites
Q84DC4

S

S

Q

A

K

L

I

V

K

R

N

H

K

R

E

R

I

I

S

T

A

S

S

R

E

Q

V

L

I

V

T

E

Q

A

F

W

T

L

D

S

N

R

I

I

E

A

Q

D

Q

F

N

S

L

-

T

E

L

L

N

N

C

A

F

F

T

I

A

S

I

V

L

D

K

A

N

A

Q

A

A

A

V

L

E

K

E

Q

A

A

K

D

N

S

I

Y

D

D

Q

H

K

Y

I

L

A

E

K

A

G

G

E

G

T

D

I

P

G

L

I

P

L

L

A

G

G

G

V

V

P

P

F

I

A

A

V

V

K
|
K

N

D

L

N

F

I

D

Q

I

V

E

V

G

G

I

F

I

A

T

N

L

T

A

A

G

G

S

T

K

P

I

A

N

L

Q

S

K

K

N

F

T

F

P

P

A

T

A

C

E

-

N

N

A

A

T

R

A

V

I

I

K

E

K

P

L

L

N

L

A

K

A

A

G

G

A

A

I

I

L

V

V

V

G

G

A

K

L

T

N

N

M

M

D

H

E

E

Y

L

A

A

Y

F

G

G

F

T

V

S

T

G

E

Y

N

N

S

T

H

A

Y

Y

G

H

-

I

-

P

-

G

-

V

-

I

A

G

T

V

P

R

N

N

P

A

H

F

D

D

L

H

S

S

R

C

I

I

S

A

G

G

S
|
S

G

S

G

G

S

S

A

G

A

T

A

A

V

V

A

G

A

A

N

L

L

L

V

I

P

P

F

A

T

A

L

L

G

G

S

T

D

D

T

T

N
x
G

G

G

S
|
S

V

V

R

R

V
x
Q

P

P

A

G

A

A

L

V

C

N

G

G

V

C

L

V

G

G

L

F

K

R

P

P

T

T

Y

V

G

G

R

R

L

Y

S

P

R

V

A

D

G

G

T

I

V

T

L

P

F

I

S

S

N

P

S

T

L

R

D

D

H

T

I

P

G

G

G

P

F

I

T

A

R

R

S

S

V

V

R

E

D

D

M

I

A

V

A

L

I

L

F

D

D

S

V

I

L

I

Q

T

G

G

E

A

D

L

K

P

T

A

D

E

-

V

P

P

V

A

C

A

T

E

S

S

L

I

A

R

P

L

Q

G

L

V

C

V

F

-

D

D

K

Q

L

L

N

W

G

A

D

D

I

L

S

S

R

E

L

P

K

V

I

R

A

K

V

L

A

T

G

E

E

D

Y

A

F

L

Q

R

K

K

G

-

A

L

E

E

T

Q

E

Q

A

G

L

V

K

Q

A

I

V

V

E

R

-

V

T

S

V

M

A

S

K

E

I

I

L

F

N

E

I

M

K
x
S

E

H

T

A

I

V

N

S

I

M

P

P

E

L

A

A

-

L

H

H

R

E

A

C

R

R

A

S

A

A

-

L

-

T

A

E

Y

Y

I

L

I

S

T

A

A

N

C

E

E

T

G

G

S

V

N

S

F

F

H

D

I

E

K

L

N

V

L

A

R

G

L

I

R

S

P

S

Q

P

D

D

F

V

D

R

T

T

A

I

T

F

R

E

D

D

R

Y

F

I

L

L

A

P

G

G

A

R

F

L

-

G

-

E

I

L

P

E

N

G

T

Q

W

S

Y

V

I

D

Q

L

A

E

Q
|
Q

R

A

F

Y

R

A

R

T

W

A

Y

M

R

K

D

D

Q

A

V

R

K

P

Q

K

I

L

F

I

Q

Q

-

S

-

F

-

E

-

F

-

L

-

F

-

K

-

E

-

H

K

Q

V

L

D

D

V

A

I

I

A

H

P

P

T

T

P

P

C

D

V

L

A

A

T

I

P

K

L

S

G

N

Q

P

E

A

T

A

M

T

N

S

I

F

N

E

G

A

E

F

E

A

I

R

L

M

I
|
I

R

-

P

-

N

-

L

-

G

-

R

R

F

N

T

A

Q

D

P

P

L

A

S

S

F

N

I

A

G

G

L

M

P

P

V

G

L

I

S

S

F

L

P

P

-

A

-

G

I

L

L

S

R

Q

P

Q

N

E

K

G

L

L

P

P

L

V

G

G

V

I

Q

E

L

I

I

E

A

G

A

L

P

P

Y

G

Q

S

E

D

L

A

K

R

I

L

L

L

Q

S

V

I

A

A

A

N

F

F

L

I

E

E

E

S

K

I

L

L

N

G

K100 (= K79) mutation to A: Abolishes activity on mandelamide.
S180 (= S155) mutation to A: Significantly decreases activity on mandelamide.
S181 (= S156) mutation to A: Significantly decreases activity on mandelamide.
G202 (≠ N177) mutation to A: Increase in KM values for aromatic substrates, but not aliphatic substrates. Active against lactamide but not against mandelamide; when associated with H-207 and E-382.; mutation to V: Increase in KM values for aromatic substrates, but not aliphatic substrates.
S204 (= S179) mutation to A: Abolishes activity on mandelamide.
Q207 (≠ V182) mutation to H: Increases activity on lactamide, does not affect activity on mandelamide; when associated with E-382. Active against lactamide but not against mandelamide; when associated with A-202 and E-382. More active on the (S)-enantiomers of mandelamide and lactamide than the (R)-enantiomers; when associated with S-316 and N-437.
S316 (≠ K291) mutation to N: More active on the (S)-enantiomers of mandelamide and lactamide than the (R)-enantiomers; when associated with H-207 and N-437.
Q382 (= Q352) mutation to H: Increases activity on lactamide, does not affect activity on mandelamide; when associated with H-207. Active against lactamide but not against mandelamide; when associated with A-202 and H-207.
I437 (= I398) mutation to N: More active on the (S)-enantiomers of mandelamide and lactamide than the (R)-enantiomers. More active on the (S)-enantiomers of mandelamide and lactamide than the (R)-enantiomers; when associated with I-31. More active on the (S)-enantiomers of mandelamide and lactamide than the (R)-enantiomers; when associated with H-207 and N-316.

Sites not aligning to the query:

31 T→I: More active on the (S)-enantiomers of mandelamide and lactamide than the (R)-enantiomers; when associated with N-437.

4yjiA The crystal structure of a bacterial aryl acylamidase belonging to the amidase signature (as) enzymes family (see paper)
29% identity, 98% coverage: 8:452/456 of query aligns to 8:474/490 of 4yjiA

query
sites
4yjiA

A

A

I

A

A

E

L

I

S

V

Q

E

K

L

I

V

K

K

N

S

K

K

E

Q

I

I

S

S

A

P

S

R

E

E

V

V

I

V

T

E

Q

S

F

T

T

I

D

D

N

L

I

I

E

E

Q

Q

Q

R

N

D

L

P

T

G

L

L

N

N

C

A

F

V

T

V

A

Y

I

K

L

A

K

Y

N

D

Q

E

A

A

V

R

E

E

E

K

A

A

K

A

N

A

I

L

D

E

Q

R

K

R

I

I

A

M

K

Q

G

G

E

E

T

P

I

V

G

G

I

M

L

L

A

A

G

G

V

V

P

P

F

T

A

L

V

M

K
|
K

N

D

L
|
L

F

F

D

A

I

A

E

K

-

P

-

G

G

W

I

P

I

S

T

T

L

L

A

G

G

G

S

I

K

R

-

A

I

L

N

K

Q

D

K

A

N

R

T

G

P

A

A

A

A

G

E

V

N

W

A

S

T

T

A

Y

I

P

K

L

K

K

L

M

N

S

A

G

A

E

G

D

A

S

I

L

L

L

V

L

G

G

A

Q

L

T

N

N

M

S

D

P

E

V

Y

Y

A
x
G

Y

F

G

R

F

G

V

T

T

T

E

D

N

N

S

T

H

F

Y

F

G

G

A

P

T

T

P

R

N

N

P

P

H

F

D

N

L

L

S

D

R

F

I

N

S

A

G

G

S
|
S

G

G

S

A

A

A

A

L

V

V

A

A

A

D

N

G

L

I

V

V

P

P

F

V

T

A

L

G

G

G

S

T

D

D

T
x
G

N
x
G

G
|
G

S
x
A

V

I

R

R

V

I

P

P

A

A

L

W

C

T

G

N

V

T

L

Y

G

G

L

F

K

Q

P

P

T

S

Y

I

G

G

R

R

L

V

-

P

-

F

-

K

S

S

R

R

A

P

G

-

T

N

V

A

L

F

F

H

S

P

N

G

S

P

L

Y

D

L

H

Y

I

E

G

G

G

P

F

I

T

T

R

R

S

T

V

V

R

R

D

D

M

A

A

A

A

L

I

A

F

M

D

N

V

V

L

L

Q

H

G

G

E

F

D

D

K

R

T

R

D

D

P

P

V

-

C

-

T

A

S

S

L

L

A

R

P

V

Q

K

L

L

C

D

F

F

-

T

D

S

K

A

L

L

N

A

G

Q

D

G

I

V

S

R

R

G

L

K

K

K

I

I

A

G

V

L

A

T

G

L

E

N

Y

Y

-

G

-

V

F

F

Q

P

K

V

G

Q

A

Q

E

E

T

I

E

Q

A

D

L

L

-

I

-

G

K

K

A

A

V

A

E

R

T

V

V

F

A

T

K

E

I

L

L

G

N

A

I

H

K

V

E

E

T

F

I

V

N

D

-

L

-

G

I

I

P

P

E

Y

A

S

H

Q

R

K

A

Q

R

M

A

S

A

D

A

A

Y

W

I

C

I

R

T

M

A

I

C

A

E

I

G

P

S

T

N

V

F

A

H

S

I

M

K

Q

N

A

L

L

R

R

L

K

R

E

P

G

Q

I

D

D

F

L

D

Y

T

G

A

E

T

H

R

R

D

-

R

-

F

-

L

-

A

-

G

-

A

A

F

D

I

I

P

P

N

D

T

A

W

L

-

M

-

K

Y

W

I

I

Q

D

A

A

-

V

-

A

-

D

-

I

-

S

-

V

-

Q

-

I

-

S

-

A

-

D

Q

Q

R

L

F

L

R

R

R

T

W

T

Y

V

R

F

D

D

Q

C

V

M

K

N

Q

G

I

V

F

F

Q

D

K

R

V

F

D

D

V

L

I

L

A

A

P

P

T

T

T

L

P

A

C

C

V

M

A

-

T

-

P

P

L

V

G

R

Q

N

E

A

T

T

-

D

-

G

-

C

-

T

-

E

-

G

-

P

M

S

N

Q

I

I

N

N

G

G

E

E

I

-

L

-

I

I

R

D

P

P

N

L

L

I

G

G

R

W

-

C

F

M

T

T

Q

Y

P

L

L

T

S

N

F

F

I

S

G

G

L

H

P

P

V

S

L

A

S

S

F

V

P

P

I

A

L

G

R

L

P

I

N

D

K

G

L

L

P

P

L

A

G

G

V

M

Q

L

L

I

I

I

A

G

A

D

P

R

Y

Q

Q

A

E

D

L

L

K

D

I

V

L

I

Q

A

V

A

A

S

A

A

F

A

L

F

E

E

E

R

active site: K79 (= K79), S158 (= S155), S159 (= S156), G179 (≠ T176), G180 (≠ N177), G181 (= G178), A182 (≠ S179)
binding n-(4-hydroxyphenyl)acetamide (tylenol): L81 (= L81), G132 (≠ A129), S158 (= S155), G179 (≠ T176), G180 (≠ N177), A182 (≠ S179)

3a1iA Crystal structure of rhodococcus sp. N-771 amidase complexed with benzamide (see paper)
28% identity, 84% coverage: 69:452/456 of query aligns to 85:498/508 of 3a1iA

query
sites
3a1iA

G

G

I

V

L

L

A

T

G

G

V

R

P

R

F

V

A

A

V

I

K
|
K

N

D

L

N

F

V

D

T

I

V

E

A

G

G

I

V

I

P

T

M

L

M

A

N

G

G

S

S

K

R

I

T

N

V

Q

E

K

G

N

F

T

T

P

P

A

S

A

-

E

R

N

D

A

A

T

T

A

V

I

V

K

T

K

R

L

L

N

L

A

A

G

G

A

A

I

T

L

V

V

A

G

G

A

K

L

A

N

V

M

C

D

E

E

D

Y

L

A

C

Y
x
F

G
x
S

F
x
G

V

S

T

S

E

F

N

T

S

P

H

A

Y

S

G

G

A

P

T

V

P

R

N

N

P

P

H

W

D

D

L

R

S

Q

R

R

I

E

S

A

G

G

S
|
S

G

G

S

S

A

A

A

L

V

V

A

A

A

N

N

G

L

D

V

V

P

D

F

F

T

A

L

I

G

G

S
x
G

D

D

T
x
Q

N
x
G

G
|
G

S
x
A

V

I

R

R

V
x
I

P

P

A

A

L

F

C

C

G

G

V

V

L

V

G

G

L

H

K

K

P

P

T

T

Y

F

G

G

R

L

L

V

S

P

R

Y

A

T

G

G

T

A

V

F

L

P

F

I

S

E

N

R

S

T

L

I

D

D

H

H

I

L

G

G

G

P

F

I

T

T

R

R

S

T

V

V

R

H

D

D

M

A

A

A

A

L

I

M

F

L

D

S

V

V

L

I

Q

A

G

G

E

R

D

D

K

G

T

N

D

D

P

P

-

R

V

Q

C

A

T

D

S

S

L

V

A

E

P

A

Q

G

L

D

C

Y

F

L

D

S

K

T

L

L

N

D

G

S

D

D

I

V

S

D

R

G

L

L

K

R

I

I

A

G

V

I

A

V

G

R

E

E

Y

G

F

F

-

G

-

H

-

A

-

V

-

S

Q

Q

K

P

G

E

A

V

E

D

T

D

E

A

A

V

L

R

K

A

A

A

V

A

E

H

T

S

V

L

A

T

K

E

I

I

L

G

N

C

I

T

K

V

E

E

T

E

I

V

N

N

I

I

P

P

E

W

A

-

H

H

R

L

A

H

R

A

A

F

A

H

A

I

Y
x
W

I

N

I

V

T

I

A

A

C

T

E

D

G

G

S

G

N

A

F

Y

-

Q

-

M

-

L

-

D

-

G

-

N

-

G

-

Y

-

G

-

M

-

N

-

A

-

E

-

G

-

L

-

Y

-

D

-

P

-

E

-

L

-

M

-

A

H

H

I

F

K

A

N

S

L

R

R

R

L

I

R

Q

P

H

Q

A

D

D

F

A

D

L

T

S

A

E

T

T

R

V

D

K

R

L

F

V

-

A

L

L

A

T

G

G

A

H

F

H

-

G

-

I

-

T

I

L

P

G

N

G

T

A

W

S

Y

Y

I

G

Q

K

A

A

Q

R

R

N

F

L

R

V

R

P

W

L

Y

A

R

R

D

A

Q

A

V

Y

K

D

Q

T

I

A

F

L

Q

R

K

Q

V

F

D

D

V

V

I

L

I

V

A

M

P

P

T

T

T

L

P

P

C

Y

V

V

A

A

T

S

P

E

L

L

G

P

Q

A

E

K

T

D

M

V

N

D

I

-

N

-

G

-

E

R

E

A

I

T

L

F

I

I

R

T

P

K

N

A

L

L

G

G

R

M

F

I

-

A

-

N

T

T

Q

A

P

P

L

F

S

D

F

V

I

T

G

G

L

H

P

P

V

S

L

L

S

S

F

V

P

P

I

A

L

G

R

L

P

V

N

N

K

G

L

L

P

P

L

V

G

G

V

M

Q

M

L

I

I

T

A

G

A

R

P

H

Y

F

Q

D

E

D

L

A

K

T

I

V

L

L

Q

R

V

V

A

G

A

R

F

A

L

F

E

E

E

K

active site: K95 (= K79), S170 (= S155), S171 (= S156), G189 (≠ S174), Q191 (≠ T176), G192 (≠ N177), G193 (= G178), A194 (≠ S179), I197 (≠ V182)
binding benzamide: F145 (≠ Y130), S146 (≠ G131), G147 (≠ F132), Q191 (≠ T176), G192 (≠ N177), G193 (= G178), A194 (≠ S179), W327 (≠ Y307)

4n0iA Crystal structure of s. Cerevisiae mitochondrial gatfab in complex with glutamine (see paper)
29% identity, 74% coverage: 98:435/456 of query aligns to 56:434/450 of 4n0iA

query
sites
4n0iA

K

E

N

N

T

F

P

K

A

S

A

P

E

F

N

D

A

A

T

T

A

V

I

V

K

K

K

L

L

L

N

K

A

Q

A

A

G

G

A

V

I

H

L

I

V

L

G

G

A

K

L

T

N

N

M

L

D

D

E

E

Y

F

A

G

Y

M

G
|
G

F

S

V

G

T

G

E

V

N

H

S

S

H

I

Y

R

G

G

A

P

T

V

P

I

N

N

P

P

-

L

-

Y

-

P

H

H

D

E

L

D

S

K

R

K

I

I

S

M

G

G

S
|
S

G

S

G

G

S

A

A

A

A

S

V

V

A

A

A

C

N

D

L

L

V

V

P

D

F

F

T

A

L

L

G

G

S
x
T

D

D

T
|
T

N
x
G

G
|
G

S
|
S

V

V

R

R

V
x
L

P

P

A

A

A

C

L

Y

C

G

G

S

V

V

L

L

G

G

L

F

K

K

P

P

T

S

Y

Y

G

G

R

R

L

L

S

S

R

R

A

F

G

G

T

V

V

I

L

A

F
x
Y

S

S

N

Q

S

S

L

L

D

D

H

T

I

V

G

G

G

I

F

L

T

S

R

K

S

K

V

I

R

N

D

V

M

L

A

R

A

K

I

V

F

F

D

H

V

T

L

L

Q

D

G

K

E

Y

D

D

K

M

T

K

D

D

P

P

V

T

C

S

T

L

S

S

L

V

A

E

P

L

Q

R

L

E

C

L

F

I

D

E

K

G

L

-

N

N

G

K

D

K

I

V

S

R

R

R

-

P

L

L

K

K

I

V

A

G

V

I

A

V

G

K

E

E

Y

F

F

S

Q

H

K

E

G

S

A

M

E

P

T

I

E

G

A

F

L

H

K

R

-

L

A

Y

V

L

E

S

T

L

V

L

A

E

K

K

I

L

L

I

N

N

I

L

K

G

E

L

T

E

I

I

-

Y

-

P

-

V

N

S

I

I

P

P

E

S

A

V

H

K

R

N

A

C

R

L

A

P

A

I

A
x
Y

Y
|
Y

I

T

I

L

T

S

A

P

C

A

E

E

-

A

-

S

-

N

-

L

-

S

-

R

-

Y

-

D

G

G

S

I

N

R

F

Y

H

G

I

Y

K

R

N

D

L

S

R

E

L

L

R

D

P

I

Q

K

D

D

-

G

-

I

-

L

-

F

-

A

-

P

-

T

-

R

-

S

-

K

F

F

D

G

T

T

A

E

T

V

R

K

D

N

R
|
R

F

I

L

I

A

L

G

G

-

N

-

Y

-

N

-

L

-

C

-

S

-

D

A

A

F

F

I

K

P

N

N

N

T

-

W

-

Y

F

I

I

Q

K

A

A

Q

E

R

K

F

L

R

R

R

V

W

N

Y

L

R

I

D

D

Q

E

V

F

K

D

Q

G

I

I

F

F

Q

R

-

F

-

P

-

N

-

V

-

L

-

T

-

N

-

S

-

K

-

G

-

N

-

P

-

D

K

G

V

L

D

D

V

L

I

L

I

I

A

V

P

P

T

T

T

-

P

-

C

-

V

-

A

S

T

S

P

K

L

L

G

P

Q

G

E

S

T

I

M

R

N

D

I

F

N

E

G

E

E

E

I

A

L

K

I

S

R

P

P

A

N

N

L

S

-

Y

-

I

-

N

G
x
D

R

V

F

F

T

T

Q

V

P

P

L

M

S

S

F

L

I

A

G

G

L

L

P

P

V

S

L

L

S

S

F

M

P

P

I

L

L

-

R

-

P

K

N

E

K

K

L

T

P

P

L

I

G

G

V

L

Q

Q

L

V

I

V

A

G

active site: S116 (= S155), S117 (= S156), T135 (≠ S174), T137 (= T176), G138 (≠ N177), G139 (= G178), S140 (= S179), L143 (≠ V182)
binding glutamine: G89 (= G131), T137 (= T176), G138 (≠ N177), S140 (= S179), Y168 (≠ F207), Y271 (≠ A306), Y272 (= Y307), R320 (= R336), D404 (≠ G403)

Sites not aligning to the query:

active site: 38

1m21A Crystal structure analysis of the peptide amidase pam in complex with the competitive inhibitor chymostatin (see paper)
33% identity, 54% coverage: 7:251/456 of query aligns to 7:256/487 of 1m21A

query
sites
1m21A

D

D

A

V

I

A

A

D

L

L

S

Q

Q

A

K

R

I

M

K

T

N

A

K

G

E

E

I

L

S

D

A

S

S

T

E

T

V

L

I

T

T

Q

Q

A

F

Y

T

L

D

Q

N

R

I

I

E

A

Q

A

Q

L

N

D

L

R

T

T

L

G

N

P

C

R

F

L

T

R

A

A

I

V

-

I

-

E

L

L

K

N

N

P

Q

D

A

A

V

L

E

K

E

E

A

A

K

A

N

E

I

R

D

D

Q

R

K

E

I

R

A

R

K

D

G

G

E

R

T

L

I

R

G

G

I

P

L

L

A

H

G

G

V

I

P

P

F

L

A

L

V

L

K
|
K

N

D

L

N

F

I

D

N

I

A

E

A

G

P

I

M

I

A

T

T

L

S

A

A

G

G

S

S

K

L

I

A

N

L

Q

Q

K

G

N

F

T

R

P

P

A

-

E

D

N

D

A

A

T

Y

A

L

I

V

K

R

L

L

N

R

A

D

A

A

G

G

A

A

I

V

L

V

V

L

G

G

A

K

L

T

N

N

M

L

D

S

E

E

Y

W

A
|
A

Y

-

G
x
N

F
|
F

V

R

T

G

E

N

N

D

S

S

-

I

-

S

-

G

-

W

-

S

-

A

H

R

Y

G

G

G

A

Q

T

T

P

R

N

N

P

P

H

Y

D

R

L

I

S

S

R

H

I

S

S

P

G
x
C

G
|
G

S
|
S

G

S

G

G

S

S

A

A

A

V

A

A

V

V

A

A

N

N

L

L

V

A

P

S

F

V

T

A

L

I

G

G

S
x
T

D

E

T
|
T

N
x
D

G
|
G

S
|
S

V

I

R

V

V
x
C

P

P

A

A

L

I

C

N

G

G

V

V

L

V

G

G

L

L

K

K

P

P

T

T

Y

V

G

G

R

L

L

V

S

S

R

R

A

D

G

G

T

I

V

I

L

P

F
x
I

S

S

N

F

S

S

L

Q

D

D

H

T

I

A

G

G

G

P

F

M

T

A

R

R

S

S

V

V

R

A

D

D

M

A

A

A

I

V

F

L

D

T

V

A

L

I

Q

A

G

G

E

R

D

D

K

D

T

A

D

D

P

P

V

A

C

T

T

A

S

T

L

M

A

P

P

G

Q

R

L

A

C

V

F

Y

D

D

active site: K81 (= K79), S160 (= S155), S161 (= S156), T179 (≠ S174), T181 (= T176), D182 (≠ N177), G183 (= G178), S184 (= S179), C187 (≠ V182)
binding : A129 (= A129), N130 (≠ G131), F131 (= F132), C158 (≠ G153), G159 (= G154), S160 (= S155), S184 (= S179), C187 (≠ V182), I212 (≠ F207)

Sites not aligning to the query:

binding : 318, 321, 365, 426

3a2qA Structure of 6-aminohexanoate cyclic dimer hydrolase complexed with substrate (see paper)
35% identity, 57% coverage: 7:264/456 of query aligns to 6:259/482 of 3a2qA

query
sites
3a2qA

D

D

A

A

I

T

A

A

L

Q

S

A

Q

E

K

L

I

V

K

R

N

S

K

G

E

E

I

I

S

S

A

R

S

T

E

E

V

L

I

L

T

E

Q

A

F

T

T

I

D

A

N

H

I

V

E

Q

Q

A

Q

V

N

N

L

P

T

E

L

I

N

N

C

A

F

V

T

I

A

I

I

P

L

L

K

F

N

E

Q

K

A

A

V

R

E

R

E

E

A

S

K

E

N

-

I

-

D

-

Q

-

K

-

I

L

A

A

K

S

G

G

E

P

T

-

I

-

G

-

I

-

L

F

A

A

G

G

V

V

P

P

F

Y

A

L

V

L

K
|
K

N

D

L

L

F

T

D

V

I

V

-

S

E

Q

G

G

I

D

I

I

T

N

L

T

A

S

G

S

S

I

K

K

-

G

I

M

N

K

Q

E

K

S

N

G

T

Y

P

R

A

A

A

D

E

H

N

D

A

A

T

Y

A

F

I

V

K

Q

K

R

L

M

N

R

A

A

G

G

A

F

I

V

L

L

V

L

G

G

A

K

L

T

N

N

M

T

D

P

E

E

Y

M

A
x
G

Y
x
N

G

Q

F

V

V

T

T

T

E

E

N

P

S

E

H

A

Y

W

G

G

A

A

T

T

P

R

N

N

P

P

H

W

D

N

L

L

S

G

R

R

I

S

S

V

G

G

S
|
S

G

G

S

S

A

G

A

A

V

V

A

A

N

A

L

L

V

S

P

P

F

V

T

A

L

H

G

G

S
x
N

D

D

T
x
A

N
x
A

G
|
G

S
x
A

V

V

R

R

V
x
I

P

P

A

A

A

S

L

V

C

C

G

G

V

V

L

V

G

G

L

L

K

K

P

P

T

T

Y

R

G

G

R

R

L

I

S

S

R

P

A

G

G

P

T

L

V

V

L

T

F

D

S

S

N

D

S

N

L

V

-

A

-

G

-

A

D

A

H

H

I

E

G

G

G

L

F

F

T

A

R

R

S

S

V

V

R

R

D

D

M

I

A

A

I

L

F

L

D

D

V

V

L

V

Q

S

G

G

E

H

D

R

K

P

T

G

D

D

P

T

V

F

C

C

T

A

S

P

L

T

A

A

P

S

Q

R

L

P

C

Y

F

A

D

Q

K

G

L

I

N

S

G

E

D

N

I

P

S

G

R

S

L

L

K

R

I

V

A

G

V

V

active site: K69 (= K79), S147 (= S155), S148 (= S156), N166 (≠ S174), A168 (≠ T176), A169 (≠ N177), G170 (= G178), A171 (≠ S179), I174 (≠ V182)
binding 6-aminohexanoic acid: G121 (≠ A129), G121 (≠ A129), N122 (≠ Y130), S147 (= S155), A168 (≠ T176), A168 (≠ T176), A169 (≠ N177), A171 (≠ S179)

Sites not aligning to the query:

binding 6-aminohexanoic acid: 313

1ocmA The crystal structure of malonamidase e2 covalently complexed with pyrophosphate from bradyrhizobium japonicum (see paper)
27% identity, 97% coverage: 11:454/456 of query aligns to 7:410/412 of 1ocmA

query
sites
1ocmA

L

L

S

Q

Q

R

K

R

I

I

K

E

N

T

K

G

E

E

I

L

S

S

A

P

S

N

E

A

V

A

I

I

T

A

Q

Q

F

S

T

H

D

A

N

A

I

I

E

E

Q

A

Q

R

N

E

L

K

T

E

L

V

N

H

C

A

F

F

T

V

A

R

I

-

L

-

K

-

N

-

Q

-

A

-

V

-

E

-

A

-

K

-

N

-

I

-

D

H

Q

D

K

K

I

S

A

A

K

R

G

A

E

Q

T

A

I

S

G

G

I

P

L

L

A

R

G

G

V

I

P

A

F

V

A

G

V

I

K
|
K

N

D

L

I

F

I

D

D

I

T

E

A

G

N

I

M

I

P

T

T

L

E

A

M

G

G

S

S

K

E

I

I

N

Y

Q

R

K

G

N

W

T

Q

P

P

A

R

A

S

E

-

N

D

A

A

T

P

A

V

I

V

K

M

L

L

N

K

A

R

A

A

G

G

A

A

I

T

L

I

V

I

G

G

A

K

L

T

N

T

M

T

D

T

E

A

Y

F

A

A

Y

S

G
x
R

F

D

V

P

T

T

E

-

N

-

S

-

H

-

Y

-

G

-

A

A

T

T

P

L

N

N

P

P

H

H

D

N

L

T

S

G

R

H

I

S

S

P

G

G

S
|
S

G

S

G

G

S

S

A

A

V

V

A

G

A

A

N

G

L

M

V

I

P

P

F

L

T

A

L

L

G

G

S

T

D
x
Q

T
|
T

N
x
G

G
|
G

S
|
S

V

V

R

I

V
x
R

P

P

A

A

L

Y

C

C

G

G

V

T

L

A

G

A

L

I

K

K

P

P

T

S

Y

F

G

R

R

M

L

L

S

P

R

T

A

V

G

G

T

V

V

K

L

C

F

Y

S

S

N

W

S

A

L

L

D

D

H

T

I

V

G

G

G

L

F

F

T

G

R

A

S

R

V

A

R

E

D

D

M

L

A

A

A

R

I

G

F

L

D

L

V

A

L

M

Q

T

G

G

E

R

D

S

K

E

T

F

D

S

P

G

V

I

C

V

T

P

S

A

L

K

A

A

P

P

Q

R

L

I

C

G

F

V

-

V

-

R

D

Q

K

E

L

F

N

A

G

G

D

A

I

V

S

E

R

P

-

A

-

E

-

Q

-

G

L

L

K

Q

I

A

A

A

V

I

-

K

A

A

G

A

E

E

Y

-

F

-

Q

R

K

A

G

G

A

A

E

S

T

V

E

Q

A

A

L

I

K

D

A

L

V

P

E

E

T

A

V

V

A

H

K

E

I

A

L

W

N

R

I

I

K

H

E

P

T

I

I

I

N

Q

I

D

P

F

E

E

A

A

H

H

R

R

A

A

R

-

A

-

Y

-

I

-

T

L

A

A

C

W

E

E

G

F

S

S

N

E

F

H

H

H

I

D

K

E

N

I

L

A

R

P

L

M

R

L

P

R

Q

A

D

S

F

L

D

D

T

A

A

T

T

V

R

-

D

-

R

-

F

-

L

-

A

-

G

-

A

G

F

L

I

T

P

P

N

K

T

E

W

-

Y

Y

I

D

Q

E

A

A

Q

R

R

R

F

I

R

G

R

R

W

R

Y

G

R

R

D

R

Q

E

V

L

K

G

Q

E

I

V

F

F

Q

E

K

G

V

V

D

D

V

V

I

L

A

T

P

Y

T

S

T

A

P

P

C

G

V

T

A

A

T

P

P

-

L

-

G

-

Q

A

E

K

T

A

M

L

N

A

I

S

N

T

G

G

E

D

E
x
P

I

-

L

-

I

-

R

-

P

-

N

-

L

-

G

-

R

R

F

Y

T

N

Q

R

P

L

L

W

S

T

F

L

I

M

G

G

L

N

P

P

V

C

L

V

S

N

F

V

P

P

I

V

L

L

R

K

P

V

N

G

K

G

L

L

P

P

L

I

G

G

V

V

Q

Q

L

V

I

I

A

A

A

R

P

F

Y

G

Q

N

E

D

L

A

K

H

I

A

L

L

Q

A

V

T

A

A

A

W

F

F

L

L

E

E

E

D

K

A

L

L

active site: K62 (= K79), S131 (= S155), S132 (= S156), T152 (= T176), G153 (≠ N177), G154 (= G178), S155 (= S179)
binding pyrophosphate 2-: R113 (≠ G131), S131 (= S155), Q151 (≠ D175), T152 (= T176), G153 (≠ N177), G154 (= G178), S155 (= S179), R158 (≠ V182), P359 (≠ E395)

Q936X2 Allophanate hydrolase; EC 3.5.1.54 from Pseudomonas sp. (strain ADP) (see paper)
28% identity, 99% coverage: 4:454/456 of query aligns to 17:465/605 of Q936X2

query
sites
Q936X2

N

D

S

H

L

L

D

T

A

D

I

L

A

A

L

S

S

Y

Q

Q

K

A

I

A

K

Y

N

A

K

A

E

G

I

T

S

D

A

A

S

A

E

D

V

V

I

I

T

S

Q

D

F

L

T

Y

D

A

N

R

I

I

E

K

Q

E

Q

D

N

G

L

E

T

N

L

P

N

I

C

W

F

I

T

S

A

L

I

L

L

P

K

L

N

E

Q

S

A

A

V

L

E

A

E

M

A

L

K

A

N

D

I

A

D

Q

Q

Q

K

R

I

K

A

D

K

K

G

G

E

E

T

A

I

L

G

P

I

L

L

F

A

-

G

G

V

I

P

P

F

F

A

G

V

V

K
|
K

N

D

L

N

F

I

D

D

I

V

E

A

G

G

I

L

I

P

T

T

L

T

A

A

G

G

S

C

K

T

I

-

N

G

Q

F

K

A

N

R

T

T

P

P

A

R

A

-

E

Q

N

H

A

A

T

F

A

V

I

V

K

Q

K

R

L

L

N

V

A

D

A

A

G

G

A

A

I

I

L

P

V

I

G

G

A

K

L

T

N

N

M

L

D

D

E

Q

Y

F

A

A

Y

T

G

G

F

L

V

N

T

G

E

T

N

R

S

T

H

P

Y

F

G

G

A

I

T

P

P

R

N

C

P

V

H

F

D

N

L

E

S

N

R

Y

I

V

S

S

G

G

S
|
S

S

S

G

S

G

G

S

S

A

A

A

V

A

A

V

V

A

A

A

N

N

G

L

T

V

V

P

P

F

F

T

S

L

L

G

G

S

T

D

D

T

T

N

A

G

G

S
|
S

V

G

R

R

V

I

P

P

A

A

L

F

C

N

G

N

V

L

L

V

G

G

L

L

K

K

P

P

T

T

Y

K

G

G

R

L

L

F

S

S

R

G

A

S

G

G

T

L

V

V

L

P

F

A

S

A

N

R

S

S

L

L

D

D

H

C

I

I

G

S

G

V

F

L

T

A

R

H

S

T

V

V

R

D

D

D

M

A

A

L

A

A

I

V

F

A

D

R

V

V

L

A

Q

A

G

G

E

Y

D

D

K

A

T

D

D

D

P

A

V

F

C

S

T

R

S

K

L

A

A

G

P

A

Q

A

L

A

C

L

F

T

D

E

K

K

L

-

N

-

G

S

D

W

I

P

S

R

R

R

L

F

K

N

I

F

A

G

V

V

-

P

A

A

G

A

E

E

Y

H

-

R

-

Q

F

F

Q

F

K

G

G

D

A

A

E

E

T

A

E

E

A

A

L

L

-

F

-

N

K

K

A

A

V

V

E

R

T

K

V

L

A

E

K

E

I

M

L

G

N

G

I

T

K

C

E

I

T

S

I

F

N

D

I

Y

P

T

E

P

A

F

H

R

R

Q

A

A

R

A

A

E

A

L

Y

Y

I

A

I

G

T

P

A

W

C

V

E

A

G

E

S

R

N

L

F

A

H

A

I

I

K

E

N

S

L

L

-

A

R

D

L

E

R

H

P

P

Q

E

D

V

F

L

D

H

T

P

A

V

T

V

R

R

D

D

R

I

F

I

L

L

A

S

G

A

A

K

F

R

I

M

P

S

N

-

T

-

W

-

Y

-

I

-

Q

A

A

V

Q

D

R

T

F

F

R

N

R

G

W

I

Y

Y

R

R

-

L

-

A

D

D

Q

L

V

V

K

R

-

A

-

E

Q

S

I

T

F

W

Q

E

K

K

V

I

D

D

V

V

I

M

I

L

A

L

P

P

T

T

T

A

P

P

C

T

V

I

A

Y

T

T

P

-

L

-

G

-

Q

-

E

-

T

V

M

E

N

D

I

M

N

L

G

A

E

D

E

P

I

V

L

R

I

L

R

N

P

S

N

N

L

L

G

G

R

F

F

Y

T

T

Q

N

P

F

L

V

S

N

F

L

I

M

G

D

L

L

P

S

V

A

L

I

S

A

F

V

P

P

I

A

-

G

L

F

R

R

P

T

N

N

K

G

L

L

P

P

L

F

G

G

V

V

Q

T

L

F

I

I

A

G

A

R

P

A

Y

F

Q

E

E

D

L

G

K

A

I

I

L

A

Q

S

V

L

A

G

-

K

A

A

F

F

L

V

E

E

E

H

K

D

L

L

K91 (= K79) mutation to A: Loss of activity.
S165 (= S155) mutation to A: Loss of activity.
S189 (= S179) mutation to A: Loss of activity.

Query Sequence

>WP_009545287.1 NCBI__GCF_000017845.1:WP_009545287.1
MNINSLDAIALSQKIKNKEISASEVITQFTDNIEQQNLTLNCFTAILKNQAVEEAKNIDQ
KIAKGETIGILAGVPFAVKNLFDIEGIITLAGSKINQKNTPAAENATAIKKLNAAGAILV
GALNMDEYAYGFVTENSHYGATPNPHDLSRISGGSSGGSAAAVAANLVPFTLGSDTNGSV
RVPAALCGVLGLKPTYGRLSRAGTVLFSNSLDHIGGFTRSVRDMAAIFDVLQGEDKTDPV
CTSLAPQLCFDKLNGDISRLKIAVAGEYFQKGAETEALKAVETVAKILNIKETINIPEAH
RARAAAYIITACEGSNFHIKNLRLRPQDFDTATRDRFLAGAFIPNTWYIQAQRFRRWYRD
QVKQIFQKVDVIIAPTTPCVATPLGQETMNINGEEILIRPNLGRFTQPLSFIGLPVLSFP
ILRPNKLPLGVQLIAAPYQELKILQVAAFLEEKLNI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory