SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_009546397.1 NCBI__GCF_000017845.1:WP_009546397.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

6j2lA Crystal structure of bi-functional enzyme (see paper)
46% identity, 57% coverage: 27:118/161 of query aligns to 15:104/200 of 6j2lA

query
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6j2lA

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binding zinc ion: D79 (= D91), D81 (= D93), D83 (= D95), C96 (= C110), C103 (= C117)

Sites not aligning to the query:

binding zinc ion: 152, 171, 174

7bgnA Crystal structure of mthisn2-amp complex, a bifunctional enzyme from the histidine biosynthetic pathway (see paper)
32% identity, 83% coverage: 16:148/161 of query aligns to 3:134/204 of 7bgnA

query
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7bgnA

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binding adenosine monophosphate: W60 (= W73), K62 (= K75), G63 (= G76), T65 (≠ S78), S66 (= S79), T94 (≠ S109), H96 (= H111), L121 (= L135), R128 (≠ T142)
binding zinc ion: D78 (= D91), D80 (= D93), D82 (= D95), C95 (= C110), C102 (= C117)

Sites not aligning to the query:

binding adenosine monophosphate: 135, 136, 137, 180, 203, 204
binding zinc ion: 154, 174, 177

7bgmA Crystal structure of mthisn2, a bifunctional enzyme from the histidine biosynthetic pathway (see paper)
36% identity, 65% coverage: 16:119/161 of query aligns to 5:106/213 of 7bgmA

query
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7bgmA

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binding zinc ion: D80 (= D91), D82 (= D93), D84 (= D95), C97 (= C110), C104 (= C117)

Sites not aligning to the query:

binding zinc ion: 163, 183, 186

6j2lB Crystal structure of bi-functional enzyme (see paper)
42% identity, 57% coverage: 27:118/161 of query aligns to 14:97/185 of 6j2lB

query
sites
6j2lB

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binding zinc ion: D78 (= D91), D80 (= D93), D82 (= D95)

Sites not aligning to the query:

binding magnesium ion: 137, 140, 156, 159

Query Sequence

>WP_009546397.1 NCBI__GCF_000017845.1:WP_009546397.1
MNPLFTARTSVEQVEEGLFLAPKFDGNGLIPVVTTDVNTGEVLMHAYMNEEALVKTIETG
EAHYYSRSRQQLWHKGQSSGLVQKVAQLTIDDDQDCLWMQVIVAGSGASCHVGYRSCFYR
RIPTGKNAIDSQQPLQLTFTETEKTFDPKAVYGDTPNPTQL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory