SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_009546633.1 NCBI__GCF_000017845.1:WP_009546633.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4hzdA Crystal structure of serine acetyltransferase in complex with coenzyme a from brucella abortus strain s19 (see paper)
43% identity, 82% coverage: 7:200/236 of query aligns to 49:248/250 of 4hzdA

query
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4hzdA

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binding coenzyme a: H158 (= H110), G203 (= G155), A204 (= A156), A221 (≠ G173), A222 (= A174), T235 (= T187), A237 (≠ V189), I244 (= I196)
binding magnesium ion: S224 (= S176), A237 (≠ V189), G238 (= G190)

1ssqD Serine acetyltransferase- complex with cysteine (see paper)
42% identity, 79% coverage: 19:204/236 of query aligns to 63:248/257 of 1ssqD

query
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binding cysteine: D88 (= D44), D153 (= D109), H154 (= H110)

8i04A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with serine (see paper)
42% identity, 79% coverage: 19:204/236 of query aligns to 63:248/258 of 8i04A

query
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binding serine: D88 (= D44), P89 (= P45), D153 (= D109), H154 (= H110), G180 (= G136), R188 (= R144), H189 (= H145)

8i09A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with butyl gallate (see paper)
42% identity, 76% coverage: 19:198/236 of query aligns to 66:245/246 of 8i09A

query
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binding cysteine: D91 (= D44), D156 (= D109), H157 (= H110), G164 (= G117), R191 (= R144), H192 (= H145)
binding butyl 3,4,5-tris(oxidanyl)benzoate: G183 (= G136), T184 (= T137), G202 (= G155), K218 (≠ R171), I219 (= I172), G220 (= G173), L226 (= L179), A236 (≠ V189)

6wyeA Crystal structure of neisseria gonorrhoeae serine acetyltransferase (cyse) (see paper)
45% identity, 71% coverage: 33:199/236 of query aligns to 82:248/261 of 6wyeA

query
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6wyeA

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binding (2S)-2-hydroxybutanedioic acid: D93 (= D44), P94 (= P45), D158 (= D109), H159 (= H110), G185 (= G136), R193 (= R144), H194 (= H145)

8i06A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with coa (see paper)
43% identity, 75% coverage: 19:196/236 of query aligns to 67:244/244 of 8i06A

query
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binding coenzyme a: H158 (= H110), L177 (≠ Y129), Q178 (= Q130), G203 (= G155), A204 (= A156), G221 (= G173), A222 (= A174), T235 (= T187), A237 (≠ V189)
binding cysteine: P93 (= P45), H158 (= H110)

3gvdI Crystal structure of serine acetyltransferase cyse from yersinia pestis
42% identity, 76% coverage: 19:198/236 of query aligns to 70:249/272 of 3gvdI

query
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3gvdI

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G

G

I

V

E

D

I

I

H

H

P

P

G

A

A

A

T

T

L

I

G

G

Q

C

G

G

V

I

F

M

I

L

D
|
D

H

H

G

A

M

T

G

G

V

I

V

V

I

I

G

G

E

E

T

T

A

A

I

V

I

V

G

E

D

N

Y

D

C

V

L

S

I

I

Y

L

Q

Q

N

S

V

V

T

T

L

L

G
|
G

G
|
G

T

T

G

G

K

K

E

T

S

S

G

G

K

D

R
|
R

H

H

P

P

T

K

L

I

G

R

N

E

S

G

V

V

I

M

V

I

G

G

A

A

G

G

A

A

K

K

V

I

L

L

G

G

N

N

I

I

E

E

I

V

G

G

N

R

H

G

V

A

R

K

I

I

G

G

A

A

G

G

S

S

I

V

V

V

L

L

S

Q

D

S

V

V

P

P

H

A

D

H

C

T

T

T

V

A

V

A

G

G

V

V

P

P

G

A

R

R

I

I

I

V

S

G

R

K

binding cysteine: D95 (= D44), P96 (= P45), D160 (= D109), G186 (= G135), G187 (= G136), R195 (= R144)

1t3dA Crystal structure of serine acetyltransferase from e.Coli at 2.2a (see paper)
40% identity, 79% coverage: 19:204/236 of query aligns to 67:252/262 of 1t3dA

query
sites
1t3dA

I

V

S

E

E

E

P

A

R

Y

T

A

S

A

R

D

P

P

N

E

L

M

L

I

S

A

F

S

L

A

R

A

K

C

D

D

F

I

Q

Q

I

A

I

V

F

R

E

T

R

R

D
|
D

P
|
P

A

A

A

V

R

D

N

K

W

Y

L

S

E

T

V

P

V

L

C

L

C

Y

Y

L

P

K

G

G

L

F

H

H

A

A

L

L

A

Q

I

A

H

Y

R

R

L

I

S

G

H

H

E

W

L

L

W

W

H

N

R

Q

N

G

L

R

P

R

F

A

F

L

P

A

R

I

F

F

L

L

S

Q

H

N

I

Q

A

V

R

S

F

V

L

T

T

F

G

Q

I

V

E

D

I

I

H

H

P

P

G

A

A

A

T

K

L

I

G

G

Q

R

G

G

V

I

F

M

I

L

D
|
D

H

H

G

A

M

T

G

G

V

I

V

V

I

V

G

G

E

E

T

T

A

A

I

V

I

I

G

E

D

N

Y

D

C

V

L

S

I

I

Y

L

Q

Q

N

S

V

V

T

T

L

L

G

G

G
|
G

T

T

G

G

K

K

E

S

S

G

G

G

K

D

R
|
R

H
|
H

P

P

T

K

L

I

G

R

N

E

S

G

V

V

I

M

V

I

G

G

A

A

G

G

A

A

K

K

V

I

L

L

G

G

N

N

I

I

E

E

I

V

G

G

N

R

H

G

V

A

R

K

I

I

G

G

A

A

G

G

S

S

I

V

V

V

L

L

S

Q

D

P

V

V

P

P

H

P

D

H

C

T

T

T

V

A

V

A

G

G

V

V

P

P

G

A

R

R

I

I

I

V

S

G

R

K

S

P

G

D

R

S

G

D

C

K

P

P

binding cysteine: D92 (= D44), P93 (= P45), D157 (= D109), G184 (= G136), R192 (= R144), H193 (= H145)

7ra4A Crystal structure of neisseria gonorrhoeae serine acetyltransferase (cyse) in complex with serine (see paper)
45% identity, 69% coverage: 33:194/236 of query aligns to 80:241/243 of 7ra4A

query
sites
7ra4A

L

V

R

E

K

A

D

D

F

L

Q

K

I

A

I

I

F

Y

E

E

R

R

D
|
D

P
|
P

A

A

A

C

R

D

N

E

W

Y

L

S

E

L

V

P

V

L

C

L

C

Y

Y

F

P

K

G

G

L

F

H

H

A

A

L

I

A

Q

I

A

H

H

R

R

L

I

S

N

H

H

E

R

L

L

W

Y

H

L

R

D

N

G

L

R

P

K

F

T

F

L

P

A

R

Y

F

F

L

L

S

Q

H

N

I

R

A

M

R

S

F

E

L

V

T

F

G

G

I

V

E

D

I

I

H

H

P

P

G

A

A

A

T

R

L

L

G

G

Q

Y

G

G

V

L

F

M

I

L

D
|
D

H
|
H

G

A

M

T

G

G

V

F

V

V

I

A

G

G

E

E

T

T

A

A

I

V

I

L

G

G

D

N

Y

N

C

I

L

S

I

I

Y

L

Q

H

N

G

V

V

T

T

L

L

G

G

G

G

T

S

G

G

K

K

E

E

S

G

G

G

K

D

R
|
R

H
|
H

P

P

T

K

L

I

G

G

N

D

S

G

V

V

I

M

V

I

G

G

A

A

G

N

A

A

K

S

V

I

L

L

G

G

N

N

I

I

E

R

I

I

G

G

N

S

H

N

V

A

R

K

I

I

G

G

A

A

G

G

S

S

I

V

V

V

L

V

S

S

D

D

V

V

P

P

H

P

D

S

C

I

T

T

V

V

V

V

G

G

V

V

P

P

G

A

R

K

binding serine: D91 (= D44), P92 (= P45), D156 (= D109), H157 (= H110), R191 (= R144), H192 (= H145)

7bw9A Crystal structure of serine acetyltransferase isoform 3 in complex with cysteine from entamoeba histolytica
41% identity, 74% coverage: 15:188/236 of query aligns to 85:258/280 of 7bw9A

query
sites
7bw9A

S

A

E

E

S

K

S

C

I

I

S

T

E

A

P

L

R

L

T

S

S

Q

R

F

P

V

N

T

L

I

L

R

S

E

F

L

L

V

R

K

K

Q

D

D

F

I

Q

I

I

A

I

A

F

Y

E

T

R

G

D
|
D

P
|
P

A

A

A

A

R

S

N

S

W

L

L

A

E

M

V

I

V

I

C

R

C

S

Y

Y

P

P

G

G

L

I

H

H

A

V

L

M

A

M

I

I

H

Q

R

R

L

V

S

A

H

H

E

I

L

L

W

Y

H

M

R

N

N

G

L

D

P

I

F

E

F

Y

P

S

R

R

F

E

L

L

S

M

H

E

I

N

A

I

R

H

F

S

L

V

T

T

G

G

I

I

E

D

I

I

H

H

P

P

G

G

A

T

T

S

L

I

G

G

Q

N

G

H

V

F

F

F

I

I

D
|
D

H
|
H

G

G

M

V

G

G

V

V

V

V

I

I

G

G

E

E

T

T

A

A

I

V

I

I

G

G

D

N

Y

W

C

C

L

R

I

V

Y

Y

Q

Q

N

S

V

V

T

T

L

L

G

G

G
x
A

T

M

G

S

K

F

E

K

S

G

G

N

K

K

R
|
R

H
|
H

P

P

T

T

L

I

G

G

N

D

S

F

V

V

I

V

V

I

G

G

A

A

G

G

A

A

K

K

V

V

L

L

G

G

N

N

I

I

E

T

I

I

G

G

N

S

H

N

V

V

R

K

I

I

G

G

A

A

G

N

S

C

I

W

V

I

L

T

S

Q

D

N

V

I

P

D

H

Q

D

D

C

Q

T

I

V

V

binding cysteine: D114 (= D44), P115 (= P45), D179 (= D109), H180 (= H110), A206 (≠ G136), R214 (= R144), H215 (= H145)

4n69A Soybean serine acetyltransferase complexed with serine (see paper)
45% identity, 73% coverage: 24:196/236 of query aligns to 71:243/243 of 4n69A

query
sites
4n69A

T

S

S

S

R

D

P

P

N

S

L

L

L

R

S

S

F

A

L

A

R

V

K

A

D

D

F

L

Q

R

I

A

I

A

F

R

E

E

R

R

D

D

P

P

A

A

A

C

R

V

N

S

W

Y

L

S

E

H

V

C

V

L

C

L

C

N

Y

Y

P

K

G

G

L

F

H

L

A

A

L

C

A

Q

I

A

H

H

R

R

L

V

S

A

H

H

E

L

L

L

W

W

H

R

R

Q

N

S

L

R

-

R

P

P

F

L

F

A

P

L

R

A

F

L

L

H

S

S

H

R

I

I

A

A

R

N

F

V

L

F

T

A

G

-

I

V

E

D

I

I

H

H

P

P

G

A

A

A

T

R

L

I

G

G

Q

K

G

G

V

I

F

L

I

F

D

D

H

H

G

A

M

T

G

G

V

V

V

V

I

V

G

G

E

E

T

T

A

A

I

V

I

I

G

G

D

N

Y

N

C

V

L

S

I

I

Y

L

Q

H

N

H

V

V

T

T

L

L

G

G

G
|
G

T

T

G

G

K

K

E

V

S

G

G

G

K

D

R
|
R

H
|
H

P

P

T

K

L

I

G

G

N

D

S

G

V

V

I

L

V

I

G

G

A

A

G

G

A

A

K

T

V

I

L

L

G

G

N

N

I

I

E

K

I

I

G

G

N

E

H

G

V

A

R

K

I

V

G

G

A

A

G

G

S

S

I

V

V

V

L

L

S

I

D

D

V

V

P

P

H

P

D

R

C

T

T

T

V

A

V

V

G

G

V

N

P

P

G

A

R

R

I

L

I

V

binding serine: G183 (= G136), R191 (= R144), H192 (= H145)

4h7oA Crystal structure of serine acetyltransferase from vibrio cholerae o1 biovar el tor n16961
41% identity, 76% coverage: 19:198/236 of query aligns to 63:242/258 of 4h7oA

query
sites
4h7oA

I

I

S

E

E

E

P

A

R

F

T

A

S

A

R

D

P

P

N

S

L

I

L

S

S

E

F

A

L

A

R

A

K

C

D

D

F

I

Q

C

I

A

I

T

F

V

E

N

R

R

D
|
D

P
|
P

A

A

A

V

R

S

N

M

W

Y

L

S

E

M

V

P

V

L

C

L

C

Y

Y

L

P

K

G

G

L

Y

H

H

A

A

L

L

A

Q

I

G

H

Y

R

R

L

V

S

A

H

N

E

W

L

L

W

W

H

R

R

Q

N

G

L

R

P

K

F

A

F

L

P

A

R

T

F
x
Y

L

F

S

Q

H

N

I
x
Q

A

I

R

S

F

V

L

A

T

C

G

Q

I

V

E

D

I

I

H

H

P

P

G

A

A

A

T

R

L

I

G

G

Q

R

G

G

V

I

F

M

I

L

D
|
D

H
|
H

G

A

M

T

G

G

V

I

V

V

I

I

G

G

E

E

T

T

A

A

I

V

I

V

G

E

D

D

Y

D

C

V

L

S

I

I

Y

L

Q

Q

N

D

V

V

T

T

L

L

G

G

G
|
G

T

T

G

G

K

K

E

E

S

C

G

G

K

D

R
|
R

H
|
H

P

P

T

K

L

I

G

R

N

E

S

G

V

V

I

M

V

I

G

G

A

A

G

G

A

A

K

K

V

I

L

L

G

G

N

N

I

I

E

E

I

V

G

G

N

E

H

G

V

A

R

K

I

I

G

G

A

S

G

G

S

S

I

V

V

V

L

L

S

Q

D

A

V

V

P

P

H

P

D

H

C

T

T

T

V

V

V

A

G

G

V

V

P

P

G

A

R

R

I

I

I

V

S

G

R

R

binding arginine: Y127 (≠ F83), Q131 (≠ I87)
binding cysteine: D88 (= D44), P89 (= P45), D153 (= D109), H154 (= H110), G180 (= G136), R188 (= R144), H189 (= H145)

Sites not aligning to the query:

binding arginine: 51, 53

3p47A Crystal structure of entamoeba histolytica serine acetyltransferase 1 in complex with l-cysteine (see paper)
45% identity, 69% coverage: 27:188/236 of query aligns to 99:268/270 of 3p47A

query
sites
3p47A

P

P

N

S

L

I

L

K

S

R

F

T

L

L

R

K

K

T

D

D

F

L

Q

I

I

A

I

A

F

Y

E

A

R

G

D

D

P

P

A

A

A

A

R

P

N

G

W

L

L

S

E

L

V

I

V

I

C

R

C

C

Y

Y

P

P

G

G

L

F

H

Q

A

A

L

V

A

I

I

V

H

Y

R

R

L

I

S

A

H

H

E

V

L

L

W

Y

H

E

R

C

N

G

L

E

P

R

F

Y

F

Y

P

C

R

R

F

E

L

M

S

M

H

E

I

S

A

V

R

H

F

S

L

Y

T

T

G

S

I

I

E

D

I

I

H

H

P

P

G

G

A

A

T

S

L

I

G

K

Q

G

G

H

V

F

F

F

I

I

D

D

H

H

G

G

M

V

G

G

V

V

V

V

I

I

G

G

E

E

T

T

A

A

I

I

I

I

G

G

D

E

Y

W

C

C

L

R

I

I

Y

Y

Q

Q

N

S

V

V

T

T

L

L

-

G

-
x
A

-

M

-
x
H

-

F

-

Q

-

E

-

E

G

G

G

G

T

V

G

I

K

K

E

R

S

G

G

T

K

K

R
|
R

H
|
H

P

P

T

T

L

V

G

G

N

D

S

Y

V

V

I

T

V

I

G

G

A

T

G

G

A

A

K

K

V

V

L

L

G

G

N

N

I

I

E

I

I

V

G

G

N

S

H

H

V

V

R

R

I

I

G

G

A

A

G

N

S

C

I

W

V

I

L

D

S

R

D

D

V

V

P

D

H

S

D

N

C

Q

T

T

V

V

binding cysteine: A208 (vs. gap), H210 (vs. gap), R224 (= R144), H225 (= H145)

3q1xA Crystal structure of entamoeba histolytica serine acetyltransferase 1 in complex with l-serine (see paper)
45% identity, 69% coverage: 27:188/236 of query aligns to 97:266/267 of 3q1xA

query
sites
3q1xA

P

P

N

S

L

I

L

K

S

R

F

T

L

L

R

K

K

T

D

D

F

L

Q

I

I

A

I

A

F

Y

E

A

R

G

D

D

P

P

A

A

A

A

R

P

N

G

W

L

L

S

E

L

V

I

V

I

C

R

C

C

Y

Y

P

P

G

G

L

F

H

Q

A

A

L

V

A

I

I

V

H

Y

R

R

L

I

S

A

H

H

E

V

L

L

W

Y

H

E

R

C

N

G

L

E

P

R

F

Y

F

Y

P

C

R

R

F

E

L

M

S

M

H

E

I

S

A

V

R

H

F

S

L

Y

T

T

G

S

I

I

E

D

I

I

H

H

P

P

G

G

A

A

T

S

L

I

G

K

Q

G

G

H

V

F

F

F

I

I

D

D

H

H

G

G

M

V

G

G

V

V

V

V

I

I

G

G

E

E

T

T

A

A

I

I

I

I

G

G

D

E

Y

W

C

C

L

R

I

I

Y

Y

Q

Q

N

S

V

V

T

T

L

L

-

G

-

A

-

M

-
x
H

-

F

-

Q

-

E

-

E

G

G

G

G

T

V

G

I

K

K

E

R

S

G

G

T

K

K

R
|
R

H
|
H

P

P

T

T

L

V

G

G

N

D

S

Y

V

V

I

T

V

I

G

G

A

T

G

G

A

A

K

K

V

V

L

L

G

G

N

N

I

I

E

I

I

V

G

G

N

S

H

H

V

V

R

R

I

I

G

G

A

A

G

N

S

C

I

W

V

I

L

D

S

R

D

D

V

V

P

D

H

S

D

N

C

Q

T

T

V

V

binding serine: H208 (vs. gap), R222 (= R144), H223 (= H145)

1sstA Serine acetyltransferase- complex with coa (see paper)
39% identity, 75% coverage: 19:196/236 of query aligns to 63:233/233 of 1sstA

query
sites
1sstA

I

I

S

E

E

E

P

A

R

Y

T

Q

S

S

R

N

P

P

N

S

L

I

L

I

S

D

F

C

L

A

R

A

K

C

D

D

F

I

Q

Q

I

A

I

V

F

R

E

H

R

R

D

D

P

P

A

A

A

V

R

E

N

L

W

W

L

S

E

T

V

P

V

L

C

L

C

Y

Y

L

P

K

G

G

L

F

H

H

A

A

L

I

A

Q

I

S

H

Y

R

R

L

I

S

T

H

H

E

Y

L

L

W

W

H

N

R

Q

N

N

L

R

P

K

F

S

F

L

P

A

R

L

F

Y

L

L

S

Q

H

N

I

Q

A

I

R

S

F

V

L

A

T

F

G

D

I

V

E

D

I

I

H

H

P

P

G

A

A

A

T

K

L

I

G

G

Q

H

G

G

V

I

F

M

I

F

D

D

H
|
H

G

A

M

T

G

G

V

I

V

V

I

V

G

G

E

E

T

T

A

S

I

V

I

I

G

E

D

N

Y

D

C

V

L

S

I

I

Y
x
L

Q

Q

N

G

V

V

T

T

L

L

G

G

G
|
G

T
|
T

G

-

K

-

E

-

S

-

G

-

K

-

R

-

H

H

P

P

T

K

L

V

G

R

N

E

S

G

V

V

I

M

V

I

G

G

A
|
A

G

G

A

A

K

K

V

I

L
|
L

G

G

N

N

I

I

E

E

I

V

G

G

N

K

H

Y

V

A

R
x
K

I

I

G
|
G

A
|
A

G

N

S

S

I
x
V

V

V

L
|
L

S

N

D

P

V

V

P

P

H

E

D

Y

C

A

T
|
T

V

A

V
x
A

G

G

V
|
V

P
|
P

G

A

R

R

I

I

I
x
V

binding coenzyme a: H154 (= H110), L173 (≠ Y129), G180 (= G136), T181 (= T137), A193 (= A156), L198 (= L161), K208 (≠ R171), G210 (= G173), A211 (= A174), V214 (≠ I177), L216 (= L179), T224 (= T187), A226 (≠ V189), V228 (= V191), P229 (= P192), V233 (≠ I196)

4n6bA Soybean serine acetyltransferase complexed with coa (see paper)
43% identity, 73% coverage: 24:196/236 of query aligns to 67:233/233 of 4n6bA

query
sites
4n6bA

T

S

S

S

R

D

P

P

N

S

L

L

L

R

S

S

F

A

L

A

R

V

K

A

D

D

F

L

Q

R

I

A

I

A

F

R

E

E

R

R

D

D

P

V

A

-

A

-

R

-

N

S

W

Y

L

S

E

H

V

C

V

L

C

L

C

N

Y

Y

P

K

G

G

L

F

H

L

A

A

L

C

A

Q

I

A

H

H

R

R

L

V

S

A

H

H

E

L

L

L

W

W

H

R

R

Q

N

S

L

R

-

R

P

P

F

L

F

A

P

L

R

A

F

L

L

H

S

S

H

R

I

I

A

A

R

N

F

V

L

F

T

-

G

A

I

V

E

D

I

I

H

H

P

P

G

A

A

A

T

R

L

I

G

G

Q

K

G

G

V

I

F

L

I

F

D

D

H
|
H

G

A

M

T

G

G

V

V

V

V

I

V

G

G

E

E

T

T

A

A

I

V

I

I

G

G

D

N

Y

N

C

V

L

S

I

I

Y
x
L

Q

H

N

H

V

V

T

T

L

L

G

G

G
|
G

T

T

G

G

K

-

E

-

S

-

G

G

K

D

R

R

H

H

P

P

T

K

L

I

G

G

N

D

S

G

V

V

I

L

V

I

G

G

A

A

G

G

A

A

K

T

V

I

L

L

G

G

N

N

I

I

E

K

I

I

G

G

N

E

H

G

V

A

R
x
K

I

V

G
|
G

A
|
A

G

G

S

S

I

V

V

V

L
|
L

S

I

D

D

V

V

P

P

H

P

D

R

C

T

T

T

V

A

V
|
V

G

G

V

N

P

P

G

A

R

R

I

L

I

V

binding coenzyme a: H150 (= H110), L169 (≠ Y129), G176 (= G136), K208 (≠ R171), G210 (= G173), A211 (= A174), L216 (= L179), V226 (= V189)

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
34% identity, 45% coverage: 90:196/236 of query aligns to 69:181/201 of 1krvA

query
sites
1krvA

F

F

L
x
S

T

Y

G

G

I

S

E

N

I

I

H

H

P

-

G

-

A

-

T

-

L

I

G

G

Q

R

G

N

V

F

F
x
Y

I

A

D
x
N

H

F

G

N

M

L

G

T

V

I

V
|
V

I

D

G
x
D

E

Y

T

T

A

V

I

T

I

I

G

G

D

D

Y

N

C

V

L

L

I

I

Y
x
A

Q

P

N

N

V

V

T

T

L

L

G
x
S

G

V

T
|
T

G

G

-

H

-

P

-

V

-

H

-

H

-

E

-

L

K

R

E

K

S

N

G

G

K

E

R
x
M

H

Y

P

S

-

F

-

P

-

I

T

T

L

I

G

G

N

N

S

N

V

V

I
x
W

V

I

G
|
G

A
x
S

G

H

A

V

K

V

V

I

L
x
N

G

P

N

G

I

V

E

T

I

I

G

G

N

D

H

N

V

S

R

V

I

I

G

G

A
|
A

G

G

S

S

I

I

V

V

L

T

S

K

D

D

V

I

P

P

H

P

D

N

C

V

T

V

V

A

V

A

G

G

V

V

P
|
P

G

C

R
|
R

I

V

I

I

binding 4-nitrophenyl beta-D-galactopyranoside: S70 (≠ L91), Y82 (≠ F107), N84 (≠ D109), V90 (= V115), D92 (≠ G117), M126 (≠ R144)
binding coenzyme a: A104 (≠ Y129), S110 (≠ G135), T112 (= T137), W138 (≠ I153), G140 (= G155), S141 (≠ A156), N146 (≠ L161), A159 (= A174), P177 (= P192), R179 (= R194)

Sites not aligning to the query:

1kruA Galactoside acetyltransferase in complex with iptg and coenzyme a (see paper)
34% identity, 45% coverage: 90:196/236 of query aligns to 69:181/201 of 1kruA

query
sites
1kruA

F

F

L
x
S

T

Y

G

G

I

S

E

N

I

I

H

H

P

-

G

-

A

-

T

-

L

I

G

G

Q

R

G

N

V

F

F
x
Y

I

A

D
x
N

H

F

G

N

M

L

G

T

V

I

V
|
V

I

D

G
x
D

E

Y

T

T

A

V

I

T

I

I

G

G

D

D

Y

N

C

V

L
|
L

I

I

Y

A

Q

P

N

N

V

V

T

T

L

L

G

S

G

V

T

T

G

G

-
x
H

-

P

-

V

-

H

-

H

-

E

-

L

K

R

E

K

S

N

G

G

K

E

R
x
M

H

Y

P

S

-

F

-

P

-

I

T

T

L

I

G

G

N

N

S

N

V

V

I
x
W

V

I

G
|
G

A
x
S

G

H

A

V

K

V

V

I

L

N

G

P

N

G

I

V

E

T

I

I

G

G

N

D

H

N

V

S

R

V

I

I

G
|
G

A
|
A

G

G

S

S

I

I

V

V

L

T

S

K

D

D

V

I

P

P

H

P

D

N

C

V

T

V

V

A

V

A

G

G

V

V

P
|
P

G

C

R

R

I

V

I

I

binding coenzyme a: H114 (vs. gap), W138 (≠ I153), G140 (= G155), S141 (≠ A156), G158 (= G173), A159 (= A174), P177 (= P192)
binding 1-methylethyl 1-thio-beta-D-galactopyranoside: S70 (≠ L91), Y82 (≠ F107), Y82 (≠ F107), N84 (≠ D109), V90 (= V115), D92 (≠ G117), L102 (= L127), H114 (vs. gap), M126 (≠ R144)

Sites not aligning to the query:

1krrA Galactoside acetyltransferase in complex with acetyl-coenzyme a (see paper)
34% identity, 45% coverage: 90:196/236 of query aligns to 69:181/200 of 1krrA

query
sites
1krrA

F

F

L

S

T

Y

G

G

I

S

E

N

I

I

H

H

P

-

G

-

A

-

T

-

L

I

G

G

Q

R

G

N

V

F

F

Y

I

A

D
x
N

H

F

G

N

M

L

G

T

V

I

V

V

I

D

G

D

E

Y

T

T

A

V

I

T

I

I

G

G

D

D

Y

N

C

V

L

L

I
|
I

Y
x
A

Q
x
P

N

N

V

V

T

T

L

L

G

S

G

V

T
|
T

G

G

-
x
H

-

P

-

V

-

H

-

H

-

E

-

L

K

R

E

K

S

N

G

G

K

E

R

M

H

Y

P

S

-

F

-

P

-

I

T

T

L

I

G

G

N

N

S

N

V

V

I

W

V

I

G
|
G

A
x
S

G

H

A

V

K

V

V

I

L
x
N

G

P

N

G

I

V

E

T

I

I

G

G

N

D

H

N

V

S

R

V

I

I

G
|
G

A
|
A

G

G

S

S

I

I

V

V

L

T

S

K

D

D

V

I

P

P

H

P

D

N

C

V

T

V

V

A

V
x
A

G

G

V

V

P
|
P

G

C

R

R

I

V

I

I

binding acetyl coenzyme *a: N84 (≠ D109), I103 (= I128), A104 (≠ Y129), P105 (≠ Q130), T112 (= T137), H114 (vs. gap), G140 (= G155), S141 (≠ A156), N146 (≠ L161), G158 (= G173), A159 (= A174), A174 (≠ V189), P177 (= P192)

Sites not aligning to the query:

binding acetyl coenzyme *a: 182

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
34% identity, 45% coverage: 90:196/236 of query aligns to 70:182/203 of P07464

query
sites
P07464

F

F

L
x
S

T

Y

G

G

I

S

E

N

I

I

H

H

P

-

G

-

A

-

T

-

L

I

G

G

Q

R

G

N

V

F

F

Y

I

A

D
x
N

H

F

G

N

M

L

G

T

V

I

V

V

I

D

G
x
D

E

Y

T

T

A

V

I

T

I

I

G

G

D

D

Y

N

C

V

L

L

I

I

Y

A

Q

P

N

N

V

V

T

T

L

L

G

S

G

V

T

T

G

G

-
x
H

-

P

-

V

-

H

-

H

-

E

-

L

K

R

E

K

S

N

G

G

K

E

R

M

H

Y

P

S

-

F

-

P

-

I

T

T

L

I

G

G

N

N

S

N

V

V

I

W

V

I

G

G

A
x
S

G

H

A

V

K

V

V

I

L

N

G

P

N

G

I

V

E

T

I

I

G

G

N

D

H

N

V

S

R

V

I

I

G

G

A
|
A

G

G

S

S

I

I

V

V

L
x
T

S
x
K

D

D

V

I

P

P

H

P

D

N

C

V

T

V

V

A

V

A

G

G

V

V

P

P

G

C

R
|
R

I

V

I

I

S71 (≠ L91) binding substrate
N85 (≠ D109) binding in other chain; binding in other chain
D93 (≠ G117) binding substrate
H115 (vs. gap) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (≠ A156) binding in other chain
A160 (= A174) binding in other chain
TK 165:166 (≠ LS 179:180) binding acetyl-CoA
R180 (= R194) binding acetyl-CoA

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial
17 binding substrate
183 binding in other chain

Query Sequence

>WP_009546633.1 NCBI__GCF_000017845.1:WP_009546633.1
MFGSLPVRERRGSPSESSISEPRTSRPNLLSFLRKDFQIIFERDPAARNWLEVVCCYPGL
HALAIHRLSHELWHRNLPFFPRFLSHIARFLTGIEIHPGATLGQGVFIDHGMGVVIGETA
IIGDYCLIYQNVTLGGTGKESGKRHPTLGNSVIVGAGAKVLGNIEIGNHVRIGAGSIVLS
DVPHDCTVVGVPGRIISRSGRGCPLEHGKLPDVEGQVIRSLLDRIEELEQKIQKLP

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory