SitesBLAST

P16250 Phosphoribosyl isomerase A; 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase; N-(5'-phosphoribosyl)anthranilate isomerase; PRAI; Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase; EC 5.3.1.16; EC 5.3.1.24 from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) (see paper)
40% identity, 98% coverage: 3:236/239 of query aligns to 6:238/240 of P16250

query
sites
P16250

L

L

Y

L

P

P

A

A

I

V

D
|
D

L

V

K

R

D

D

G

G

Q

Q

C

A

V

V

R
|
R

L

L

L

V

R

H

G

G

E

E

M

S

E

G

A

T

A

E

T

T

V

S

F

Y

G

G

D

S

D

-

P

P

A

L

A

E

Q

A

A

A

A

L

A

A

F

W

E

Q

A

R

A

S

G

G

C

A

E

E

W

W

V

L

H

H

L

L

V

V

D

D

L

L

N

D

G

A

A

A

F

F

A

-

G

G

R

T

P

G

V

D

N

N

A

R

A

A

A

L

V

I

E

A

A

E

I

V

L

A

A

Q

R

A

I

M

A

D

V

I

P

K

A

V

Q

E

L

L

G
x
S

G

G

I

I

R

R

D

D

M

D

A

D

T

T

I

L

A

A

L

A

W

A

L

L

E

A

K

T

G

G

L

C

A

T

R

R

V

V

I

N

L

L

G

G

T

T

V

A

A

A

V

L

E

E

N

T

P

P

G

E

L

W

V

V

R

A

E

K

A

V

A

I

R

A

A

E

F

H

P

G

G

D

R

K

V

I

A

A

V

V

G

G

I

L

D
|
D

A

V

R

R

K

G

G

T

R

T

V

L

A

R

T

G

K

R

G

G

W

W

A

T

T

R

E

D

T

G

D

G

V

D

M

L

A

Y

T

E

D

T

L

L

A

D

R

R

S

-

F

L

E

N

D

K

A

E

G

G

V

C

A

A

A

R

I

Y

I

V

Y

V

T
|
T

D

D

I

I

D

A

R

K

D
|
D

G

G

A

T

M

L

A

Q

G

G

P

P

N

N

I

L

E

E

A

L

T

L

D

K

A

N

L

V

A

C

R

A

A

A

V

T

S

D

I

R

P

P

V

V

I

V

A

A

S

S

G

G

V

V

S

S

L

L

A

D

D

D

L

L

L

R

A

A

L

I

R

A

D

G

-

L

-

V

T

P

G

A

S

G

I

V

A

E

G

G

A

A

I

I

S

V

G

G

R

K

A

A

L

L

Y

Y

D

A

G

K

A

A

L

F

D

T

L

L

T

E

Q

E

A

A

L

L

Q

E

A

A

D11 (= D8) mutation to A: No activity.
R19 (= R16) mutation to A: No effect on activity toward PRA. No activity toward ProFAR.
S81 (≠ G80) mutation to T: No activity toward PRA. Almost no effect on activity toward ProFAR.
D130 (= D129) mutation to A: Very low activity toward PRA. No activity toward ProFAR.; mutation to Q: No activity.
T166 (= T166) mutation to A: No activity.
D171 (= D171) mutation to A: Low activity toward PRA. No activity toward ProFAR.

4tx9A Crystal structure of hisap from streptomyces sviceus with degraded profar (see paper)
41% identity, 98% coverage: 3:237/239 of query aligns to 11:244/246 of 4tx9A

query
sites
4tx9A

L

L

Y

L

P

P

A

A

I

V

D
|
D

L

V

K

R

D

D

G

G

Q

Q

C

A

V

V

R

R

L

L

L

V

R

H

G
|
G

E

E

M

S

E

G

A

T

A

E

T

T

V

S

F

Y

G

G

D

S

D

-

P

P

A

L

A

E

Q

A

A

A

A

L

A

S

F

W

E

Q

A

R

A

A

G

G

C

A

E

E

W

W

V

L

H

H

L

L

V
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V

D

D

L
|
L

N

D

G

A

A

A

F

F

A

-

G

G

R

T

P

G

V

D

N

N

A

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A

E

A

L

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V

E

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T

A

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A

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M

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I

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A

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E

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L

G
x
S

G

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G
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G

I

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R

D

D

M

D

A

A

T

S

I

L

A

A

L

A

W

A

L

L

E

A

K

T

G

G

L

C

A

T

R

R

V

V

I

N

L

L

G
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G

T
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T

V

A

A

A

V

L

E

E

N

S

P

P

G

E

L

W

V

V

R

A

E

K

A

V

A

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R

A

A

E

F

H

P

G

G

D

R

R

V

I

A

A

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V

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G

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L

D
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D

A

V

R

R

K

G

G

T

R

T

V

L

A

K

T

G

K

R

G
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G

W
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W

A

T

T

S

E

E

T

G

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G

V

D

M

L

A

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T

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D

A

L

L

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F

L

E

D

D

K

A

E

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G

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C

A

A

A

R

I

Y

I

V

Y

V

T

T

D

D

I

I

D

A

R

K

D

D

G

G

A

T

M

L

A

Q

G

G

P

P

N

N

I

L

E

E

A

L

T

L

D

R

A

N

L

V

A

C

R

A

A

A

V

T

S

D

I

R

P

P

V

V

I

V

A

A

S

S

G

G

V

V

S

S

L

L

A

D

D

D

L

L

L

R

A

A

L

I

R

A

D

E

T

L

G

V

-

P

-

L

S

G

I

V

A

E

G

G

A

S

I

I

S

V

G

G

R

K

A

A

L

L

Y

Y

D

A

G

K

A

A

L

F

D

T

L

L

T

E

Q

E

A

A

L

L

Q

E

A

A

L

V

active site: D16 (= D8), D135 (= D129)
binding aminoimidazole 4-carboxamide ribonucleotide: G28 (= G20), V57 (= V50), L59 (= L52), S86 (≠ G80), G88 (= G82), R90 (= R84), G109 (= G103), T110 (= T104), D135 (= D129), G145 (= G139), W146 (= W140)

3zs4A Crystal structure of mycobacterium tuberculosis phosphoribosyl isomerase with bound prfar
38% identity, 100% coverage: 1:238/239 of query aligns to 3:243/244 of 3zs4A

query
sites
3zs4A

M

L

I

I

L

L

Y

L

P

P

A
|
A

I

V

D
|
D

L

V

K

V

D

E

G

G

Q

R

C

A

V

V

R
|
R

L

L

L

V

R

Q

G

G

E

K

M

A

E

G

A

S

A

Q

T

T

V

E

F

Y

G

G

D

S

D

A

P

V

A

D

A

A

Q

-

A

A

A

L

A

G

F

W

E

Q

A

R

A

D

G

G

C

A

E

E

W

W

V

I

H

H

L

L

V

V

D

D

L

L

N

D

G

A

A
|
A

F
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F

A

-

G

G

R

R

P

G

V

S

N

N

A

H

A

E

A

L

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L

E

A

A

E

I

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L

V

A

G

R

K

I

L

A

D

V

V

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Q

A

V

Q

E

L

L

G
x
S

G

G

G
|
G

I

I

R
|
R

D

D

M

D

A

E

T

S

I

L

A

A

L

A

W

A

L

L

E

A

K

T

G

G

L

C

A

A

R

R

V

V

I

N

L

V

G
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G

T
|
T

V

A

A

A

V

L

E

E

N

N

P

P

G

Q

L

W

V

C

R

A

E

R

A

V

A

I

R

G

A

E

F

H

P

G

G

D

R

Q

V

V

A

A

V

V

G

G

I

L

D
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D

A

V

R

Q

-

I

-

D

-

G

K

E

G

H

R

R

V

L

A

R

T

G

K

R

G
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G

W
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W

A

E

T

T

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D

T

G

D

G

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D

M

L

A

-

T

W

D

D

L

V

A

L

R

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F

L

E

D

D

S

A

E

G

G

V

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A

S

A

R

I

F

I

V

Y

V

T

T

D

D

I

I

D

T

R

K

D
|
D

G
|
G

A

T

M

L

A

G

G

G

P

P

N

N

I

L

E

D

A

L

T

L

D

A

A

G

L

V

A

A

R

D

A

R

V

T

S

D

I

A

P

P

V

V

I

I

A

A

S

S

G
|
G

V

V

S

S

L

L

A

D

D

D

L

L

-

R

-

A

L

I

A

A

L

T

R

L

D

T

T

H

G

R

S

G

I

V

A

E

G

G

A

A

I
|
I

S

V

G
|
G

R
x
K

A

A

L

L

Y

Y

D

A

G

R

A

R

L

F

D

T

L

L

T

P

Q

Q

A

A

L

L

Q

A

A

A

L

V

R

R

active site: D10 (= D8), D129 (= D129)
binding phosphoric acid mono-[5-({[5-carbamoyl-3-(5-phosphonooxy-5-deoxy-ribofuranosyl)- 3h-imidazol-4-ylamino]-methyl}-amino)-2,3,4-trihydroxy-pentyl] ester: A8 (= A6), D10 (= D8), R18 (= R16), A56 (= A55), F57 (= F56), S80 (≠ G80), G82 (= G82), R84 (= R84), G103 (= G103), T104 (= T104), D129 (= D129), G143 (= G139), W144 (= W140), D174 (= D171), G175 (= G172), G200 (= G197), G201 (= G198), I223 (= I218), G225 (= G220), K226 (≠ R221)

2y88A Crystal structure of mycobacterium tuberculosis phosphoribosyl isomerase (variant d11n) with bound prfar (see paper)
38% identity, 100% coverage: 1:238/239 of query aligns to 3:243/244 of 2y88A

query
sites
2y88A

M

L

I

I

L

L

Y

L

P

P

A
|
A

I

V

D
x
N

L

V

K

V

D

E

G

G

Q

R

C

A

V

V

R
|
R

L

L

L

V

R

Q

G
|
G

E

K

M

A

E

G

A

S

A

Q

T

T

V

E

F

Y

G

G

D

S

D

A

P

V

A

D

A

A

Q

-

A

A

A

L

A

G

F

W

E

Q

A

R

A

D

G

G

C

A

E

E

W

W

V

I

H

H

L

L

V

V

D

D

L
|
L

N

D

G

A

A
|
A

F

F

A

-

G

G

R

R

P

G

V

S

N

N

A

H

A

E

A

L

V

L

E

A

A

E

I

V

L

V

A

G

R

K

I

L

A

D

V

V

P

Q

A

V

Q

E

L

L

G
x
S

G
|
G

I

I

R
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R

D

D

M

D

A

E

T

S

I

L

A

A

L

A

W

A

L

L

E

A

K

T

G

G

L

C

A

A

R

R

V

V

I

N

L

V

G
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G

T
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T

V

A

A

A

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L

E

E

N

N

P

P

G

Q

L

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V

C

R

A

E

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A

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A

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R

G

A

E

F

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P

G

G

D

R

Q

V

V

A

A

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G

G

I

L

D
|
D

A

V

R

Q

-

I

-

D

-

G

K

E

G

H

R

R

V

L

A

R

T

G

K

R

G
|
G

W
|
W

A

E

T

T

E

D

T

G

D

G

V

D

M

L

A

-

T

W

D

D

L

V

A

L

R

E

S

R

F

L

E

D

D

S

A

E

G

G

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C

A

S

A

R

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F

I

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Y

V

T

T

D

D

I

I

D

T

R

K

D
|
D

G
|
G

A

T

M

L

A

G

G

G

P

P

N

N

I

L

E

D

A

L

T

L

D

A

A

G

L

V

A

A

R

D

A

R

V

T

S

D

I

A

P

P

V

V

I

I

A

A

S
|
S

G
|
G

G

G

V

V

S

S

L

L

A

D

D

D

L

L

-

R

-

A

L

I

A

A

L

T

R

L

D

T

T

H

G

R

S

G

I

V

A

E

G

G

A

A

I

I

S

V

G
|
G

R
x
K

A

A

L

L

Y

Y

D

A

G

R

A

R

L

F

D

T

L

L

T

P

Q

Q

A

A

L

L

Q

A

A

A

L

V

R

R

active site: N10 (≠ D8), D129 (= D129)
binding [(2r,3s,4r,5r)-5-[4-aminocarbonyl-5-[[(z)-[(3r,4r)-3,4-dihydroxy-2-oxo-5-phosphonooxy-pentyl]iminomethyl]amino]imidazol-1-yl]-3,4-dihydroxy-oxolan-2-yl]methyl dihydrogen phosphate: A8 (= A6), N10 (≠ D8), R18 (= R16), G22 (= G20), L53 (= L52), A56 (= A55), S80 (≠ G80), G81 (= G81), G82 (= G82), R84 (= R84), G103 (= G103), T104 (= T104), D129 (= D129), G143 (= G139), W144 (= W140), D174 (= D171), G175 (= G172), S199 (= S196), G200 (= G197), G225 (= G220), K226 (≠ R221)

2y85A Crystal structure of mycobacterium tuberculosis phosphoribosyl isomerase with bound rcdrp (see paper)
37% identity, 100% coverage: 1:238/239 of query aligns to 3:234/234 of 2y85A

query
sites
2y85A

M

L

I

I

L

L

Y

L

P

P

A

A

I

V

D
|
D

L

V

K

V

D

E

G

G

Q

R

C

A

V

V

R

R

L

T

L

E

R

Y

G

G

E

S

M

A

E

V

A

D

A

A

T

-

V

-

F

-

G

-

D

-

P

-

A

-

Q

-

A

A

A

L

A

G

F

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E

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A

R

A

D

G

G

C

A

E

E

W

W

V

I

H
|
H

L

L

V
|
V

D

D

L
|
L

N

D

G

A

A
|
A

F

F

A

-

G

G

R

R

P

G

V

S

N

N

A

H

A

E

A

L

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L

E

A

A

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I

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V

A

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I

L

A

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A

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L

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x
S

G

G

I

I

R

R

D

D

M

D

A

E

T

S

I

L

A

A

L

A

W

A

L

L

E

A

K

T

G

G

L

C

A

A

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R

V

V

I

N

L

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G

G

T

T

V

A

A

A

V

L

E

E

N

N

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P

G

Q

L

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C

R

A

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R

A

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A

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G

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F

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P

G

G

D

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V

V

A

A

V

V

G

G

I

L

D
|
D

A

V

R

Q

-

I

-

D

-

G

K

E

G

H

R

R

V

L

A

R

T

G

K
x
R

G

G

W

W

A

E

T

T

E

D

T

G

D

G

V

D

M

L

A

-

T

W

D

D

L

V

A

L

R

E

S

R

F

L

E

D

D

S

A

E

G

G

V

C

A

S

A

R

I

F

I

V

Y

V

T

T

D

D

I

I

D

T

R

K

D
|
D

G
|
G

A

T

M

L

A

G

G

G

P

P

N

N

I

L

E

D

A

L

T

L

D

A

A

G

L

V

A

A

R

D

A

R

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D

I

A

P

P

V

V

I

I

A

A

S
|
S

G
|
G

V

V

S

S

L

L

A

D

D

D

L

L

-

R

-

A

L

I

A

A

L

T

R

L

D

T

T

H

G

R

S

G

I

V

A

E

G

G

A

A

I

I

S

V

G
|
G

R
x
K

A

A

L

L

Y

Y

D

A

G

R

A

R

L

F

D

T

L

L

T

P

Q

Q

A

A

L

L

Q

A

A

A

L

V

R

R

active site: D10 (= D8), D120 (= D129)
binding 1-(o-carboxy-phenylamino)-1-deoxy-d-ribulose-5-phosphate: D10 (= D8), H40 (= H48), V42 (= V50), L44 (= L52), A47 (= A55), S71 (≠ G80), R133 (≠ K138), D165 (= D171), G166 (= G172), S190 (= S196), G191 (= G197), G192 (= G198), G216 (= G220), K217 (≠ R221)

5abtA S.Enterica hisa mutant d7n, g102a, v106m, d176a
31% identity, 100% coverage: 2:239/239 of query aligns to 1:244/246 of 5abtA

query
sites
5abtA

I

M

L

I

Y

I

P

P

A

A

I

L

D
x
N

L

L

K

I

D

D

G

G

Q

T

C

V

V

V

R

R

L

L

L

H

R

Q

G
|
G

E

D

M

Y

E

A

A

R

A

Q

T

R

V

D

F

Y

G

G

D

N

D

D

P

P

A

L

A

P

Q

R

A

L

A

Q

A

D

F

Y

E

A

A

A

A

Q

G

G

C

A

E

G

W

V

V

L

H

H

L

L

V
|
V

D

D

L
|
L

N

T

G

G

A

A

F

K

A

D

G

P

R

A

P

K

V

R

N

Q

A

I

A

P

A

L

V

I

E

K

A

T

I

L

L

V

A

A

R

G

I

V

A

N

V

V

P

P

A

V

Q

Q

L

V

G

G

G
|
G

I

V

R
|
R

D

T

M

E

A

E

T

D

I

V

A

A

L

A

W

L

L

L

E

K

K

A

G

G

L

V

A

A

R

R

V

V

I
x
V

L

I

G
x
A

T
x
S

V

T

A

A

V

M

E

K

N

S

P

P

G

D

L

V

V

V

R

K

E

G

A

W

A

F

R

E

A

R

F

F

P

G

G

A

R

Q

-

A

V

L

A

V

V

L

G

A

I

L

D
|
D

A

V

R

R

-

I

-

D

-

E

-

H

-

G

K

T

G

K

R

Q

V

V

A

A

T

V

K

S

G
|
G

W
|
W

A

Q

T

E

E

N

T

S

D

G

V

V

M

S

A

L

T

E

D

Q

L

L

A

V

R

E

S

T

F

Y

E

L

D

P

A

V

G

G

V

L

A

K

A

H

I

V

I

L

Y

C

T

T

D

D

I

I

D

S

R

R

D

A

G
|
G

A

T

M

L

A

A

G

G

P

S

N

N

I

V

E

S

A

L

T

Y

D

E

A

E

L

V

-

C

A

A

R

R

A

Y

V

P

S

Q

I

I

P

A

V

F

I

Q

A

S

S
|
S

G
|
G

G

G

V

I

S

G

S

D

L

I

A

D

D

D

L

I

L

A

A

A

L

L

R

R

D

G

T

T

G

G

S

-

I

V

A

R

G

G

A

V

I
|
I

S

V

G
|
G

R
|
R

A

A

L

L

Y

L

D

E

G

G

A

K

L

F

D

T

L

V

T

K

Q

E

A

A

L

I

Q

Q

A

C

L

W

R

Q

T

N

active site: N7 (≠ D8), D129 (= D129)
binding [(2R,3S,4R,5R)-5-[4-aminocarbonyl-5-[(E)-[[(2R,3R,4S,5R)-3,4-bis(oxidanyl)-5-(phosphonooxymethyl)oxolan-2-yl]amino]methylideneamino]imidazol-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl dihydrogen phosphate: N7 (≠ D8), G19 (= G20), V49 (= V50), L51 (= L52), G81 (= G82), R83 (= R84), V100 (≠ I101), A102 (≠ G103), S103 (≠ T104), D129 (= D129), G144 (= G139), W145 (= W140), G177 (= G172), S202 (= S196), G203 (= G197), I223 (= I218), G225 (= G220), R226 (= R221)

Q9X0C7 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase; Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase; EC 5.3.1.16 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
29% identity, 95% coverage: 1:228/239 of query aligns to 1:231/241 of Q9X0C7

query
sites
Q9X0C7

M

M

I

L

L

V

Y

V

P

P

A

A

I

I

D
|
D

L

L

K

F

D

R

G

G

Q

K

C

V

V

A

R

R

L

M

L

I

R

K

G

G

E

R

M

K

E

E

A

N

A

T

T

I

V

F

F

Y

G

E

D

K

D

D

P

P

A

V

A

E

Q

L

A

V

A

E

A

K

F

L

E

I

A

E

G

G

C

F

E

T

W

L

V

I

H
|
H

L

V

V

V

D
|
D

L

L

N

S

G

N

A

A

F

I

A

E

G

N

R

S

P

G

V

E

N

N

A

L

A

P

A

V

V

L

E

E

A

K

I

-

L

L

A

S

R

E

I

F

A

A

V

E

P

H

A

I

Q

Q

L

I

G

G

I

I

R
|
R

D

S

M

L

A

D

T

Y

I

A

A

E

L

K

W

L

L

R

E

K

K

L

G

G

L

Y

A

R

R

R

V

Q

I

I

L

V

G

S

T

S

V

K

A

V

V

L

E

E

N

D

P

P

G

S

L

F

V

L

R

K

E

-

A

S

A

L

R

R

A

E

F

I

P

D

G

V

R

E

V

P

A

V

V

F

G

S

I

L

D
|
D

A

T

R

R

K

G

G

G

R

R

V

V

A

A

T

F

K

K

G

G

W

W

A

L

T

A

E

E

T

E

D

E

V

I

M

D

A

P

T

V

D

S

L

L

A

L

R

K

S

R

F

L

E

K

D

E

A

Y

G

G

V

L

A

E

I

I

I

V

Y

H

T
|
T

D

E

I

I

D

E

R

K

D

D

G

G

A

T

M

L

A

Q

G

E

P

H

N

D

I

F

E

S

A

L

T

T

D

K

A

K

L

I

A

A

R

I

A

E

V

A

S

E

I

V

P

K

V

V

I

L

A

A

S

A

G

G

V

I

S

S

L

E

A

N

D

S

L

L

-

K

-

T

-

A

-

Q

-

K

L

V

A

H

L

T

R

E

D

T

T

N

G

G

S

L

I

L

A

K

G

G

A

V

I

I

S

V

G

G

R

R

A

A

L

F

Y

L

D

E

G

G

A

I

L

L

D8 (= D8) mutation to N: Loss of activity.
H48 (= H48) mutation to A: Decrease in activity.
D51 (= D51) mutation to N: Decrease in activity.
R83 (= R84) mutation to N: Decrease in activity.
D127 (= D129) mutation to N: Almost no activity.
T164 (= T166) mutation to A: Strong decrease in activity.

5ab3A S.Enterica hisa mutant d7n, d10g, dup13-15, q24l, g102a (see paper)
32% identity, 98% coverage: 2:236/239 of query aligns to 1:238/241 of 5ab3A

query
sites
5ab3A

I

M

L

I

Y

I

P

P

A

A

I

L

D
x
N

L

L

K

I

D

G

G

G

Q

T

C

V

V

V

R

R

L

V

R
|
R

-

L

-

H

-

Q

G

G

E

D

M
x
Y

E

A

A

R

A

L

T

R

V

D

F

Y

G

G

D

N

D

D

P

P

A

L

A

P

Q

R

A

L

A

Q

A

D

F

Y

E

A

A

A

A

Q

G

G

C

A

E

G

W

V

V

L

H

H

L

L

V
|
V

D

D

L

L

N

T

G

G

A

A

F

K

A

D

G

P

R

A

P

K

V

R

N

Q

A

I

A

P

A

L

V

I

E

K

A

T

I

L

L

V

A

A

R

G

I

V

A

N

V

V

P

P

A

V

Q

Q

L

V

G

G

G
|
G

I

V

R

R

D

T

M

E

A

E

T

D

I

V

A

A

L

A

W

L

L

L

E

K

K

A

G

G

L

V

A

A

R

R

V

V

I

V

L

I

G
x
A

T
x
S

V

T

A

A

V

V

E

K

N

S

P

P

G

D

L

V

V

V

R

K

E

G

A

W

A

F

R

E

A

R

F

F

P

G

G

A

R

Q

-

A

V

L

A

V

V

L

G
x
A

I

L

D
|
D

A

V

R

R

K

I

G

D

R

E

V

H

A

G

T

T

K

K

G

Q

W

V

A

A

T

V

E

-

T

S

D

G

V

V

M

S

A

L

T

E

D

Q

L

L

A

V

R

E

S

T

F

Y

E

L

D

P

A

V

G

G

V

L

A

K

A

H

I

V

I

L

Y

C

T

T

D

D

I

I

D

S

R

R

D

D

G
|
G

A

T

M

L

A

A

G

G

P

S

N

N

I

V

E

S

A

L

T

Y

D

E

A

E

L

V

-

C

A

A

R

R

A

Y

V

P

S

Q

I

I

P

A

V

F

I

Q

A

S

S
|
S

G
|
G

V

I

S

G

S

D

L

I

A

D

D

D

L

I

L

A

A

A

L

L

R

R

D

G

T

T

G

G

S

-

I

V

A

R

G

G

A

V

I

I

S

V

G
|
G

R
|
R

A

A

L

L

Y

L

D

E

G

G

A

K

L

F

D

T

L

V

T

K

Q

E

A

A

L

I

Q

Q

A

C

active site: N7 (≠ D8), D132 (= D129)
binding [(2r,3s,4r,5r)-5-[4-aminocarbonyl-5-[[(z)-[(3r,4r)-3,4-dihydroxy-2-oxo-5-phosphonooxy-pentyl]iminomethyl]amino]imidazol-1-yl]-3,4-dihydroxy-oxolan-2-yl]methyl dihydrogen phosphate: N7 (≠ D8), R18 (= R19), Y24 (≠ M22), V52 (= V50), G84 (= G82), A105 (≠ G103), S106 (≠ T104), A130 (≠ G127), D132 (= D129), G174 (= G172), S199 (= S196), G200 (= G197), G201 (= G198), G222 (= G220), R223 (= R221)

3zr4E Structural evidence for ammonia tunneling across the (beta-alpha)8 barrel of the imidazole glycerol phosphate synthase bienzyme complex (see paper)
28% identity, 84% coverage: 6:206/239 of query aligns to 9:202/244 of 3zr4E

query
sites
3zr4E

A

C

I

L

D
|
D

L

V

K

K

D

D

G

G

Q

R

C

V

V

V

R

K

L

-

R

-

G

-

E

-

M

-

E

-

A

-

T

-

V

-

F

-

G

S

D

G

D

D

P

P

A

V

A

E

Q

L

A

G

A

K

A

F

F

Y

E

S

A

E

A

I

G

G

C

I

E

D

W

E

V

L

H

V

L

F

V

L

D

D

L

I

N

T

G

A

A

S

F

V

A

E

G

K

R

R

P

K

V

T

N

M

A

L

A

E

A

L

V

V

E

E

A

K

I

V

L

A

A

E

R

Q

I

I

A

D

V

I

P

P

A

F

Q

T

L

V

G

G

I

I

R

H

D

D

M

F

A

E

T

T

I

A

A

S

L

E

W

L

L

I

E

L

K

R

G

G

L

A

A

D

R

K

V

V

I

S

L

I

G

N

T

T

V

A

A

A

V

V

E

E

N

N

P

P

G

S

L

L

V

I

R

T

E

Q

A

I

A

A

R

Q

A

T

F

F

P

G

G

S

R

Q

-

A

V

V

A

V

V

V

G

A

I

I

D
|
D

A

A

R

K

K

R

-

V

-

D

-

G

-

E

G

F

R

M

V

V

A

F

T

T

K

Y

G

S

W

G

A

K

T

K

E

N

T

T

D

G

V

I

M

L

A

L

T

R

D

D

L

W

A

V

R

V

S

E

F

V

E

E

D

K

A

R

G

G

V

A

A

G

A

E

I

I

I

L

Y

L

T

T

D

S

I

I

D

D

R

R

D

D

G

G

A

T

M

K

A

S

G

G

P

Y

N

D

I

T

E

E

A

M

T

I

D
x
R

A

F

L

V

A

R

R

P

A

L

V

T

S

T

I

L

P

P

V

I

I

I

A

A

S

S

G

G

V

A

S

G

S

K

L

M

A

E

D

H

L

F

L

L

active site: D11 (= D8), D121 (= D129)
binding glycerol: R179 (≠ D183)

Sites not aligning to the query:

binding glycerol: 206

Q9X0C6 Imidazole glycerol phosphate synthase subunit HisF; IGP synthase cyclase subunit; IGP synthase subunit HisF; ImGP synthase subunit HisF; IGPS subunit HisF; EC 4.3.2.10 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
28% identity, 84% coverage: 6:206/239 of query aligns to 9:211/253 of Q9X0C6

query
sites
Q9X0C6

A
x
C

I

L

D
|
D

L

V

K

K

D

D

G

G

Q

R

C

V

V

V

R
x
K

L

-

R

-

G

-

E

-

M

-

E

-

A

-

A

G

T

T

V

N

F

F

G

E

D

N

-

L

-

R

-

D

-

S

-

G

D

D

P

P

A

V

A

E

Q

L

A

G

A

K

A

F

F

Y

E

S

A

E

A

I

G

G

C

I

E

D

W

E

V

L

H

V

L

F

V

L

D
|
D

L

I

N

T

G

A

A

S

F

V

A

E

G

K

R

R

P

K

V

T

N

M

A

L

A

E

A

L

V

V

E

E

A

K

I

V

L

A

A

E

R

Q

I

I

A

D

V

I

P

P

A

F

Q

T

L

V

G

G

I

I

R

H

D

D

M

F

A

E

T

T

I

A

A

S

L

E

W

L

L

I

E

L

K

R

G

G

L

A

A

D

R

K

V

V

I

S

L

I

G
x
N

T

T

V

A

A

A

V

V

E

E

N

N

P

P

G

S

L

L

V

I

R

T

E

Q

A

I

A

A

R

Q

A

T

F

F

P

G

G

S

R

Q

-

A

V

V

A

V

V

V

G

A

I

I

D
|
D

A

A

R

K

K

R

-

V

-

D

-

G

-

E

G

F

R

M

V

V

A

F

T

T

K

Y

G

S

W

G

A

K

T

K

E

N

T

T

D

G

V

I

M

L

A

L

T

R

D

D

L

W

A

V

R

V

S

E

F

V

E

E

D

K

A

R

G

G

V

A

A

G

A

E

I

I

I

L

Y

L

T

T

D

S

I

I

D

D

R

R

D
|
D

G

G

A

T

M

K

A

S

G

G

P

Y

N
x
D

I

T

E

E

A

M

T

I

D

R

A

F

L

V

A

R

R

P

A

L

V

T

S

T

I

L

P

P

V

I

I

I

A

A

S

S

G

G

V

A

S

G

S

K

L

M

A

E

D

H

L

F

L

L

C9 (≠ A6) mutation to A: No change in activity.
D11 (= D8) mutation to X: Loss of activity.
K19 (≠ R16) mutation to S: Decrease in activity.
D51 (= D51) mutation to N: No change in activity.
N103 (≠ G103) mutation to A: No change in activity.
D130 (= D129) mutation D->A,C,F,G,H,I,K,L,M,N,P,Q,R,S,T,V,W,Y: Complete loss of activity.; mutation to E: Weak activity.
D176 (= D171) mutation to N: Decrease in activity.
D183 (≠ N178) mutation to N: No change in activity.

7ac8A Molecular basis for the unique allosteric activation mechanism of the heterodimeric imidazole glycerol phosphate synthase complex. (see paper)
28% identity, 84% coverage: 6:206/239 of query aligns to 9:211/252 of 7ac8A

query
sites
7ac8A

A

C

I

L

D
|
D

L

V

K

K

D

D

G

G

Q

R

C

V

V

V

R

K

L

-

R

-

G

-

E

-

M

-

E

-

A

-

A

G

T

T

V

N

F

F

G

E

D

N

-

L

-

R

-

D

-

S

-

G

D

D

P

P

A

V

A

E

Q

L

A

G

A

K

A

F

F

Y

E

S

A

E

A

I

G

G

C

I

E

D

W

E

V

L

H

V

L

F

V
x
L

D

D

L
x
I

N

T

G

A

A

S

F

V

A

E

G

K

R

R

P

K

V

T

N

M

A

L

A

E

A

L

V

V

E

E

A

K

I

V

L

A

A

E

R

Q

I

I

A

D

V

I

P

P

A

F

Q

T

L

V

G

G

G
|
G

I

I

R

H

D

D

M

F

A

E

T

T

I

A

A

S

L

E

W

L

L

I

E

L

K

R

G

G

L

A

A

D

R

K

V

V

I

S

L

I

G
x
N

T
|
T

V

A

A

A

V

V

E

E

N

N

P

P

G

S

L

L

V

I

R

T

E

Q

A

I

A

A

R

Q

A

T

F

F

P

G

G

S

R

Q

-

A

V

V

A

V

V

V

G

A

I

I

D
|
D

A

A

R

K

K

R

-

V

-

D

-

G

-

E

G

F

R

M

V

V

A

F

T

T

K

Y

G
x
S

W

G

A

K

T

K

E

N

T

T

D

G

V

I

M

L

A

L

T

R

D

D

L

W

A

V

R

V

S

E

F

V

E

E

D

K

A

R

G

G

V

A

A

G

A

E

I

I

I

L

Y

L

T

T

D

S

I

I

D

D

R

R

D
|
D

G
|
G

A

T

M

K

A

S

G

G

P

Y

N

D

I

T

E

E

A

M

T

I

D

R

A

F

L

V

A

R

R

P

A

L

V

T

S

T

I

L

P

P

V

I

I

I

A

A

S
|
S

G

G

V

A

S

G

S

K

L

M

A

E

D

H

L

F

L

L

active site: D11 (= D8), D130 (= D129)
binding [(2R,3S,4R,5R)-5-[4-aminocarbonyl-5-[(E)-[[(2R,3R,4S,5R)-3,4-bis(oxidanyl)-5-(phosphonooxymethyl)oxolan-2-yl]amino]methylideneamino]imidazol-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl dihydrogen phosphate: L50 (≠ V50), I52 (≠ L52), G82 (= G82), N103 (≠ G103), T104 (= T104), D130 (= D129), S144 (≠ G139), D176 (= D171), G177 (= G172), S201 (= S196)

Sites not aligning to the query:

binding [(2R,3S,4R,5R)-5-[4-aminocarbonyl-5-[(E)-[[(2R,3R,4S,5R)-3,4-bis(oxidanyl)-5-(phosphonooxymethyl)oxolan-2-yl]amino]methylideneamino]imidazol-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl dihydrogen phosphate: 225

7qc8A Imidazole glycerol phosphate synthase subunit HisF (see paper)
29% identity, 84% coverage: 6:206/239 of query aligns to 9:211/250 of 7qc8A

query
sites
7qc8A

A

A

I

L

D
x
E

L

V

K

K

D

D

G

G

Q

R

C

V

V

V

R

K

L

-

R

-

G

-

E

-

M

-

E

-

A

-

A

G

T

T

V

N

F

F

G

E

D

N

-

L

-

R

-

D

-

S

-

G

D

D

P

P

A

A

A

E

Q

L

A

G

A

K

A

F

F

Y

E

S

A

E

A

I

G

G

C

I

E

D

W

E

V

L

H

V

L

F

V
x
H

D

D

L
x
H

N

T

G

A

A

S

F

V

A

E

G

K

R

R

P

K

V

T

N

M

A

L

A

E

A

L

V

V

E

E

A

K

I

V

L

A

A

E

R

Q

I

I

A

D

V

I

P

P

A

F

Q

T

L

V

G

G

I

I

R

H

D

D

M

F

A

E

T

T

I

A

A

S

L

E

W

L

L

I

E

L

K

R

G

G

L

A

A

D

R

K

V

V

I

S

L

I

G

N

T

T

V

A

A

A

V

V

E

E

N

N

P

P

G

S

L

L

V

I

R

T

E

Q

A

I

A

A

R

Q

A

T

F

F

P

G

G

S

R

Q

-

A

V

V

A

V

V

V

G

A

I

I

D

D

A

A

R

K

K

R

-

V

-

D

-

G

-

E

G

F

R

M

V

V

A

F

T

T

K

Y

G

S

W

G

A

K

T

K

E

N

T

T

D

G

V

I

M

L

A

L

T

R

D

D

L

W

A

V

R

V

S

E

F

V

E

E

D

K

A

R

G

G

V

A

A

G

A

E

I

I

I

L

Y

L

T

T

D

S

I

I

D

D

R

R

D

D

G

G

A

T

M

K

A

S

G

G

P

Y

N

D

I

T

E

E

A

M

T

I

D

R

A

F

L

V

A

R

R

P

A

L

V

T

S

T

I

L

P

P

V

I

I

I

A

A

S

S

G

G

V

A

S

G

S

K

L

M

A

E

D

H

L

F

L

L

binding zinc ion: E11 (≠ D8), H50 (≠ V50), H52 (≠ L52)

1gpwC Structural evidence for ammonia tunneling across the (beta/alpha)8 barrel of the imidazole glycerol phosphate synthase bienzyme complex. (see paper)
28% identity, 81% coverage: 14:206/239 of query aligns to 9:211/253 of 1gpwC

query
sites
1gpwC

C

C

V

L

R
x
N

L

V

L

K

R

D

G

G

E

R

M

V

E

V

A

K

A

G

T

T

V

N

F

F

G

E

D

N

-

L

-

R

-

D

-

S

-

G

D

D

P

P

A

V

A

E

Q

L

A

G

A

K

A

F

F

Y

E

S

A

E

A

I

G

G

C

I

E

D

W

E

V

L

H

V

L

F

V

L

D

D

L

I

N

T

G

A

A

S

F

V

A

E

G

K

R

R

P

K

V

T

N

M

A

L

A

E

A

L

V

V

E

E

A

K

I

V

L

A

A

E

R

Q

I

I

A

D

V

I

P

P

A

F

Q

T

L

V

G

G

G
|
G

I

I

R

H

D

D

M

F

A

E

T

T

I

A

A

S

L

E

W

L

L

I

E

L

K

R

G

G

L

A

A

D

R

K

V

V

I

S

L

I

G
x
N

T
|
T

V

A

A

A

V

V

E

E

N

N

P

P

G

S

L

L

V

I

R

T

E

Q

A

I

A

A

R

Q

A

T

F

F

P

G

G

S

R

Q

-

A

V

V

A

V

V

V

G

A

I

I

D
|
D

A

A

R

K

K

R

-

V

-

D

-

G

-

E

G

F

R

M

V

V

A

F

T

T

K

Y

G

S

W

G

A

K

T

K

E

N

T

T

D

G

V

I

M

L

A

L

T

R

D

D

L

W

A

V

R

V

S

E

F

V

E

E

D

K

A

R

G

G

V

A

A

G

A

E

I

I

I

L

Y

L

T

T

D

S

I

I

D

D

R

R

D
|
D

G
|
G

A

T

M

K

A

S

G

G

P

Y

N

D

I

T

E

E

A

M

T

I

D

R

A

F

L

V

A

R

R

P

A

L

V

T

S

T

I

L

P

P

V

I

I

I

A

A

S

S

G

G

V

A

S

G

S

K

L

M

A

E

D

H

L

F

L

L

active site: N11 (≠ R16), D130 (= D129)
binding phosphate ion: G82 (= G82), N103 (≠ G103), T104 (= T104), D176 (= D171), G177 (= G172)

Sites not aligning to the query:

binding phosphate ion: 225

1h5yB Hisf protein from pyrobaculum aerophilum (see paper)
34% identity, 70% coverage: 31:198/239 of query aligns to 34:206/253 of 1h5yB

query
sites
1h5yB

D

D

P

P

A

V

A

E

Q

M

A

A

A

V

A

R

F

Y

E

E

A

E

G

G

C

A

E

D

W

E

V

I

H

A

L

I

V

L

D

D

L

I

N

T

G

A

A

A

F

P

A

E

G

G

R

R

P

A

V

T

N

F

A

I

A

D

A

S

V

V

E

K

A

R

I

V

L

A

A

E

R

A

I

V

A

S

V

I

P

P

A

V

Q

L

L

V

G

G

G
|
G

I

V

R

R

D

S

M

L

A

E

T

D

I

A

A

T

L

T

W

L

L

F

E

R

K

A

G

G

L

A

A

D

R

K

V

V

I

S

L

V

G
x
N

T
|
T

V

A

A

A

V

V

E

R

N

N

P

P

G

Q

L

L

V

V

R

A

E

L

A

L

A

A

R

R

A

E

F

F

P

G

G

S

R

Q

-

S

V

T

A

V

V

V

G
x
A

I

I

D
|
D

A

A

R

K

K

W

G

N

-

G

-

E

-

Y

R

E

V

V

A

Y

T

V

K

K

G
|
G

W

G

A

R

T

E

E

A

T

T

D

G

V

L

M

D

A

A

T

V

D

K

L

W

A

A

R

K

S

E

F

V

E

E

D

E

A

L

G

G

V

A

A

G

A

E

I

I

I

L

Y

L

T
|
T

D

S

I

I

D

D

R

R

D
|
D

G
|
G

A

T

M

G

A

L

G

G

P

Y

N

D

I

V

E

E

A

L

T

I

D

R

A

R

L

V

A

A

R

D

A

S

V

V

S

R

I

I

P

P

V

V

I

I

A

A

S

S

G

G

active site: D133 (= D129)
binding glycerol: N106 (≠ G103), A131 (≠ G127), D133 (= D129), G147 (= G139), T174 (= T166), D179 (= D171)
binding phosphate ion: G84 (= G81), G85 (= G82), N106 (≠ G103), T107 (= T104), D179 (= D171), G180 (= G172)

Sites not aligning to the query:

2wjzE Crystal structure of (hish) k181a y138a mutant of imidazoleglycerolphosphate synthase (hish hisf) which displays constitutive glutaminase activity (see paper)
29% identity, 84% coverage: 6:206/239 of query aligns to 9:197/237 of 2wjzE

query
sites
2wjzE

A

C

I

L

D
|
D

L

V

K

K

D

D

G

G

Q

R

C

V

V

V

R

-

L

-

R

-

G

-

E

-

M

-

E

-

A

-

T

-

V

-

F

-

G

-

D

G

D

D

P

P

A

V

A

E

Q

L

A

G

A

K

A

F

F

Y

E

S

A

E

A

I

G

G

C

I

E

D

W

E

V

L

H

V

L

F

V

L

D

D

L

I

N

T

G

-

A

-

F

-

A

A

G

K

R

R

P

K

V

T

N

M

A

L

A

E

A

L

V

V

E

E

A

K

I

V

L

A

A

E

R

Q

I

I

A

D

V

I

P

P

A

F

Q

T

L

V

G

G

G
|
G

I

I

R

H

D

D

M

F

A

E

T

T

I

A

A

S

L

E

W

L

L

I

E

L

K

R

G

G

L

A

A

D

R

K

V

V

I

S

L

I

G
x
N

T
|
T

V

A

A

A

V

V

E

E

N

N

P

P

G

S

L

L

V

I

R

T

E

Q

A

I

A

A

R

Q

A

T

F

F

P

G

G

S

R

Q

-

A

V

V

A

V

V

V

G

A

I

I

D
|
D

A

A

R

K

K

R

-

V

-

D

-

G

-

E

G

F

R

M

V

V

A

F

T

T

K

Y

G

S

W

G

A

K

T

K

E

N

T

T

D

G

V

I

M

L

A

L

T

R

D

D

L

W

A

V

R

V

S

E

F

V

E

E

D

K

A

R

G

G

V

A

A

G

A

E

I

I

I

L

Y

L

T

T

D

S

I

I

D

D

R

R

D

D

G

G

A

T

M

K

A

S

G

G

P

Y

N

D

I

T

E

E

A

M

T

I

D

R

A

F

L

V

A

R

R

P

A

L

V

T

S

T

I

L

P

P

V

I

I

I

A

A

S

S

G

G

V

A

S

G

S

K

L

M

A

E

D

H

L

F

L

L

active site: D11 (= D8), D116 (= D129)
binding phosphate ion: G68 (= G82), N89 (≠ G103), T90 (= T104)

4ewnD Structure of hisf-d130v+d176v with bound rcdrp (see paper)
27% identity, 84% coverage: 6:206/239 of query aligns to 8:204/243 of 4ewnD

query
sites
4ewnD

A

C

I

L

D

D

L

V

K

K

D

D

G

G

Q

R

C

V

V

V

R

K

L

-

R

-

G

-

E

-

M

-

E

-

A

-

A

G

T

T

V

N

F

F

G

E

D

N

-

L

-

R

-

D

-

S

-

G

D

D

P

P

A

V

A

E

Q

L

A

G

A

K

A

F

F

Y

E

S

A

E

A

I

G

G

C

I

E

D

W

E

V

L

H

V

L

F

V

L

D

D

L

I

N

T

G

K

A

T

F

M

A

L

G

-

R

-

P

-

V

-

N

-

A

-

A

E

A

L

V

V

E

E

A

K

I

V

L

A

A

E

R

Q

I

I

A

D

V

I

P

P

A

F

Q

T

L

V

G

G

I

I

R

H

D

D

M

F

A

E

T

T

I

A

A

S

L

E

W

L

L

I

E

L

K

R

G

G

L

A

A

D

R

K

V

V

I

S

L

I

G

N

T

T

V

A

A

A

V

V

E

E

N

N

P

P

G

S

L

L

V

I

R

T

E

Q

A

I

A

A

R

Q

A

T

F

F

P

G

G

S

R

Q

-

A

V

V

A

V

V

V

G

A

I

I

D
x
V

A

A

R

K

K

R

-

V

-

D

-

G

-

E

G

F

R

M

V

V

A

F

T

T

K

Y

G

S

W

G

A

K

T

K

E

N

T

T

D

G

V

I

M

L

A

L

T

R

D

D

L

W

A

V

R

V

S

E

F

V

E

E

D

K

A

R

G

G

V

A

A

G

A

E

I

I

I

L

Y

L

T

T

D

S

I

I

D

D

R

R

D

V

G
|
G

A

T

M

K

A

S

G

G

P

Y

N

D

I

T

E

E

A

M

T

I

D

R

A

F

L

V

A

R

R

P

A

L

V

T

S

T

I

L

P

P

V

I

I

I

A

A

S
|
S

G
|
G

V

A

S

G

S

K

L

M

A

E

D

H

L

F

L

L

binding 1-(O-carboxy-phenylamino)-1-deoxy-D-ribulose-5-phosphate: V123 (≠ D129), G170 (= G172), S194 (= S196), G195 (= G197), G196 (= G198)

Sites not aligning to the query:

binding 1-(O-carboxy-phenylamino)-1-deoxy-D-ribulose-5-phosphate: 215, 216, 217, 218

5d2tA Directed evolutionary changes in kemp eliminase ke07 - crystal 3 wild type
27% identity, 84% coverage: 6:206/239 of query aligns to 7:209/251 of 5d2tA

query
sites
5d2tA

A

A

I

L

D

I

L

V

K

K

D

D

G

G

Q

-

C

-

V

-

R

R

L

V

R

K

G

G

E

S

M

N

E

F

A

E

A

N

T

L

V

R

F

D

G

S

D

G

D

D

P

P

A

V

A

E

Q

L

A

G

A

K

A

F

F

Y

E

S

A

E

A

I

G

G

C

I

E

D

W

E

V

L

H

S

L

F

V
x
W

D

D

L

I

N

T

G

A

A

S

F

V

A

E

G

K

R

R

P

K

V

T

N

M

A

L

A

E

A

L

V

V

E

E

A

K

I

V

L

A

A

E

R

Q

I

I

A

D

V

I

P

P

A

F

Q

T

L

V

G

G

I

I

R

H

D

D

M

F

A

E

T

T

I

A

A

S

L

E

W

L

L

I

E

L

K

R

G

G

L

A

A

D

R

K

V

V

I
x
E

L

I

G

N

T

T

V

A

A

A

V

V

E

E

N

N

P

P

G

S

L

L

V

I

R

T

E

Q

A

I

A

A

R

Q

A

T

F

F

P

G

G

S

R

Q

-

A

V

V

A

V

V

V

G
x
Y

I

I

D

A

A

A

R

K

K

R

-

V

-

D

-

G

-

E

G

F

R

M

V

V

A

F

T

T

K

Y

G

S

W

G

A

K

T

K

E

N

T

T

D

G

V

I

M

L

A

L

T

R

D

D

L

W

A

V

R

V

S

E

F

V

E

E

D

K

A

R

G

G

V

A

A

G

A

E

I

I

I

V

Y

L

T

G

D

S

I

I

D

D

R

R

D

L

G

G

A

T

M

K

A

S

G

G

P

Y

N

D

I

T

E

E

A

M

T

I

D

R

A

F

L

V

A

R

R

P

A

L

V

T

S

T

I

L

P

P

V

I

I

I

A

A

S

H

G

G

V

A

S

G

S

K

L

M

A

E

D

H

L

F

L

L

binding 5-nitro-2-oxidanyl-benzenecarbonitrile: W48 (≠ V50), E99 (≠ I101), Y126 (≠ G127)

Sites not aligning to the query:

binding 5-nitro-2-oxidanyl-benzenecarbonitrile: 220

6dnjA Directed evolutionary changes in kemp eliminase ke07 - crystal 28 round 5 (see paper)
26% identity, 84% coverage: 6:206/239 of query aligns to 8:210/250 of 6dnjA

query
sites
6dnjA

A

A

I

L

D

I

L

M

K

K

D

D

G

G

Q

-

C

-

V

-

R

R

L

V

R

K

G

G

E

S

M

N

E

F

A

E

A

N

T

L

V

R

F

D

G

S

D

G

D

D

P

P

A

V

A

E

Q

L

A

G

A

K

A

F

F

Y

E

S

A

E

A

I

G

G

C

I

E

D

W

E

V

L

H

S

L

F

V
x
W

D

D

L

I

N

T

G

A

A

S

F

V

A

E

G

K

R

R

P

K

V

T

N

M

A

L

A

E

A

L

V

V

E

E

A

K

I

V

L

A

A

E

R

Q

I

I

A

D

V

I

P

P

A

F

Q

T

L

V

G
|
G

G

G

I

I

R

H

D

D

M

F

A

E

T

T

I

A

A

S

L

E

W

L

L

I

E

L

K

R

G

G

L

A

A

D

R

K

V

V

I

E

L

I

G
x
N

T

T

V

A

A

A

V

V

E

E

N

N

P

P

G

S

L

L

V

I

R

T

E

Q

A

I

A

A

R

Q

A

T

F

F

P

G

G

S

R

Q

-

A

V

V

A

V

V

V

G
x
Y

I

I

D

A

A

A

R

K

K

R

-

V

-

D

-

G

-

E

G

F

R

M

V

V

A

F

T

T

K

Y

G

S

W

G

A

K

T

K

E

N

T

T

D

G

V

I

M

L

A

L

T

R

D

D

L

W

A

V

R

V

S

E

F

V

E

E

D

K

A

R

G

G

V

A

A

G

A

E

I

I

I

V

Y

L

T

G

D

S

I

I

D

D

R

R

D

L

G

G

A

T

M

K

A

S

G

G

P

Y

N

D

I

T

E

E

A

M

T

I

D

R

A

F

L

V

A

R

R

P

A

L

V

T

S

T

I

L

P

P

V

I

I

I

A

A

S

H

G

R

G

G

V

A

S

G

S

K

L

M

A

E

D

H

L

F

L

L

binding 5-nitro-1,2-benzoxazole: W49 (≠ V50), G79 (= G80), G80 (= G81), N102 (≠ G103), Y127 (≠ G127)

P60664 Imidazole glycerol phosphate synthase subunit HisF; IGP synthase cyclase subunit; IGP synthase subunit HisF; ImGP synthase subunit HisF; IGPS subunit HisF; EC 4.3.2.10 from Escherichia coli (strain K12) (see paper)
24% identity, 95% coverage: 3:230/239 of query aligns to 6:241/258 of P60664

query
sites
P60664

L

I

Y

I

P

P

A

C

I

L

D

D

L

V

K

R

D

D

G

G

Q

Q

C

V

V

V

R

K

L

G

L

V

R

-

G

-

E

Q

M

F

E

R

A

N

A

H

T

E

V

I

F

I

G

G

D

D

D

I

-

V

P

P

A

L

A

A

Q

K

A

R

A

Y

A

A

F

E

E

E

A

-

G

G

C

A

E

D

W
x
E

V

L

H

V

L

F

V

Y

D

D

L

I

N

T

G

A

A

S

F

S

A

D

G

G

R

R

P

V

V

V

N

D

A

K

A

S

A

W

V

V

E

S

A

R

I

V

L

A

A

E

R

V

I

I

A

D

V

I

P

P

A

F

Q

C

L

V

G

A

G

G

I

I

R

K

D

S

M

L

A

E

T

D

I

A

A

A

L

K

W

I

L

L

E

S

K

F

G

G

L

A

A

D

R

K

V

I

I

S

L

I

G

N

T

S

V

P

A

A

V

L

E

A

N

D

P

P

G

T

L

L

V

I

R

T

E

R

A

L

A

A

R

D