SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_009566656.1 NCBI__GCF_000021485.1:WP_009566656.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 12 hits to proteins with known functional sites (download)

P0A7B5 Glutamate 5-kinase; Gamma-glutamyl kinase; GK; EC 2.7.2.11 from Escherichia coli (strain K12) (see paper)
41% identity, 75% coverage: 29:278/332 of query aligns to 8:257/367 of P0A7B5

query
sites
P0A7B5

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S50 (= S71) binding substrate
D137 (= D158) binding substrate
N149 (= N170) binding substrate

2j5tD Glutamate 5-kinase from escherichia coli complexed with glutamate (see paper)
41% identity, 75% coverage: 29:278/332 of query aligns to 6:255/365 of 2j5tD

query
sites
2j5tD

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binding glutamic acid: S48 (= S71), G49 (= G72), A50 (≠ S73), N132 (= N155), D135 (= D158), D146 (≠ N169), N147 (= N170)

Sites not aligning to the query:

binding glutamic acid: 282, 285

2j5vB Glutamate 5-kinase from escherichia coli complexed with glutamyl-5- phosphate and pyroglutamic acid (see paper)
34% identity, 75% coverage: 29:278/332 of query aligns to 6:215/325 of 2j5vB

query
sites
2j5vB

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binding pyroglutamic acid: T11 (≠ S34), G49 (= G72), A50 (≠ S73), I51 (≠ V74), A52 (≠ S75), D135 (= D158)
binding gamma-glutamyl phosphate: K8 (= K31), S48 (= S71), D135 (= D158), G145 (= G168), D146 (≠ N169), N147 (= N170)

2j5vA Glutamate 5-kinase from escherichia coli complexed with glutamyl-5- phosphate and pyroglutamic acid (see paper)
33% identity, 75% coverage: 29:278/332 of query aligns to 6:213/323 of 2j5vA

query
sites
2j5vA

V

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binding magnesium ion: K8 (= K31), G10 (= G33), L166 (= L189)
binding pyroglutamic acid: T11 (≠ S34), S48 (= S71), G49 (= G72), A50 (≠ S73), I51 (≠ V74)
binding gamma-glutamyl phosphate: K8 (= K31), G10 (= G33), S48 (= S71), D135 (= D158), D146 (≠ N169), N147 (= N170)

2akoA Crystal structure of glutamate 5-kinase from campylobacter jejuni
33% identity, 70% coverage: 26:257/332 of query aligns to 1:219/241 of 2akoA

query
sites
2akoA

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M

I

L

V

T

T

K
|
K

I

L

H

K

A

A

A

A

T

K

H

F

A

L

A

L

R

E

S

H

G

N

A

K

T

K

T

M

L

F

I

L

A

A

D

S

G

G

binding adenosine-5'-diphosphate: H10 (≠ S35), S161 (≠ T190), D162 (= D191), I163 (≠ Q192), F166 (≠ L195), Y167 (≠ F196), N170 (≠ D199), P171 (= P200), T194 (= T232), G196 (= G234), K200 (= K238)

8j0gB Gk monomer complexes with glutamate and atp
30% identity, 77% coverage: 20:276/332 of query aligns to 2:267/274 of 8j0gB

query
sites
8j0gB

A

A

L

F

Q

A

Q

K

R

D

A

V

R

K

R

R

W

I

V

V

I

V

K
|
K

I

V

G
|
G

S
x
T

S
x
A

L

V

L

V

T

T

Q

G

D

K

G

G

Q

G

R

R

L

L

D

A

L

L

S

G

A

R

M

L

Q

G

K

A

L

I

V

C

Q

E

Q

Q

I

L

L

A

R

E

L

L

R

N

A

S

E

D

D

G

I

F

E

E

V

V

V

I

L

L

V

V

S

S

S

S

G

G

S
x
A

V

V

S

G

A

L

G

G

K

R

C

Q

H

R

L

L

G

R

W
x
Y

A

R

Q

Q

K
x
L

M
x
V

T

N

T

S

A

S

E
x
F

-

A

-

D

-

L

-

Q

-

K

-

P

-

Q

-

M

-

E

-

L

E

D

R

G

Q

K

A

A

A

C

A

A

S

G

V

V

E

G

Q

Q

S

S

A

S

L

L

I

M

H

A

A

Y

Y

Y

E

E

N

T

L

M

L

F

A

D

E

Q

G

L

G

D

T

V

H

T

C

V

G

A

Q

Q

I

M

L

L

L

V

T

T

R

D

D

S

N

S

F

F

R

R

D

D

R

K

K

D

R

F

L

R

K

K

K

Q

T

L

R

S

S

E

A

T

I

V

E

K

M

A

L

M

L

L

R

R

M

M

Q

R

M

V

L

I

P

P

I

V

I

F

N
|
N

E

E

N

N

D
|
D

A

A

I

I

V

S

D

T

L

R

D

R

N

T

A

G

L

I

G

F

-

W

N

D

N
|
N

D

D

H

S

L

L

A

A

A

A

L

L

V

L

S

S

N

L

V

E

W

L

H

K

A

A

D

D

L

L

M

L

V

I

L

L

L

L

T

S

D

D

Q
x
V

S

E

G

G

L

L

F

Y

T

T

A
x
G

D

P

P
|
P

R

-

A

-

R

S

P

D

D

S

A

T

E

S

M

K

I

L

T

I

E

H

G

T

M

F

A

I

G

K

D

E

P

K

R

H

Y

Q

E

D

H

E

M

I

A

T

G
x
F

G

G

S

E

G

K

G

S

T

K

V

L

G

G

T
x
R

G
|
G

G
|
G

M
|
M

L

T

T

A

K
|
K

I

V

H

K

A

A

A

A

T

V

H

N

A

A

A

A

R

Y

S

G

G

G

A

V

T

P

T

V

L

I

I

I

A

T

D

S

G

G

R

Y

I

A

P

A

D

E

V

N

L

I

L

S

R

K

L

V

H

L

K

R

G

G

A

L

L

R

L

V

G

G

T

T

F

L

L

F

binding adenosine-5'-triphosphate: K13 (= K31), G15 (= G33), T16 (≠ S34), A17 (≠ S35), V185 (≠ Q192), G191 (≠ A198), P193 (= P200), F215 (≠ G224), R223 (≠ T232), G225 (= G234), M226 (= M235), K229 (= K238)
binding gamma-l-glutamic acid: A55 (≠ S73), N147 (= N155), D150 (= D158), N163 (= N170), R223 (≠ T232)
binding magnesium ion: R223 (≠ T232), G224 (= G233), G225 (= G234)
binding : Y65 (≠ W83), L68 (≠ K86), V69 (≠ M87), F73 (≠ E91)

8j0eB Gk monomer complexes with catalytic intermediate
34% identity, 55% coverage: 17:200/332 of query aligns to 1:192/269 of 8j0eB

query
sites
8j0eB

D

D

R

R

A

S

-

R

A

A

L

F

Q

A

Q

K

R

D

A

V

R

K

R

R

W

I

V

V

I

V

K
|
K

I

V

G
|
G

S
x
T

S
x
A

L

V

L

V

T

T

Q

G

D

K

G

G

Q

G

R

R

L

L

D

A

L

L

S

G

A

R

M

L

Q

G

K

A

L

I

V

C

Q

E

Q

Q

I

L

L

A

R

E

L

L

R

N

A

S

E

D

D

G

I

F

E

E

V

V

V

I

L

L

V

V

S

S

S
|
S

G
|
G

S

A

V
|
V

S

G

A

L

G

G

K

R

C

Q

H

R

L

L

G

R

W
x
Y

A

R

Q

Q

K
x
L

M
x
V

T

N

T
x
S

A

S

E
x
F

-

A

-

D

-

L

-
x
Q

-

K

-

P

-

Q

-

M

-

E

-

L

E

D

R

G

Q

K

A

A

A

C

A

A

S

G

V

V

E

G

Q

Q

S

S

A

S

L

L

I

M

H

A

A

Y

Y

Y

E

E

N

T

L

M

L

F

A

D

E

Q

G

L

G

D

T

V

H

T

C

V

G

A

Q

Q

I

M

L

L

L

V

T

T

R

D

D

S

N

S

F

F

R

R

D

D

R

K

K

D

R

F

L

R

K

K

K

Q

T

L

R

S

S

E

A

T

I

V

E

K

M

A

L

M

L

L

R

R

M

M

Q

R

M

V

L

I

P

P

I

V

I

F

N

N

E

E

N

N

D
|
D

A

A

I

I

V

-

D

-

L

-

D

S

N

T

A

R

L

F

G

W

N

D

N
|
N

D
|
D

H

S

L

L

A

A

A

A

L

L

V

L

S

S

N

L

V

E

W

L

H

K

A

A

D

D

L

L

M

L

V

I

L

L

L

L

T

S

D

D

Q
x
V

S

E

G

G

L

L

F

Y

T

T

A

G

D

P

P
|
P

binding magnesium ion: D163 (= D171)
binding (2~{R})-5-[[[[(2~{R},3~{S},4~{S},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-2-azanyl-5-oxidanylidene-pentanoic acid: K16 (= K31), G18 (= G33), T19 (≠ S34), A20 (≠ S35), S56 (= S71), G57 (= G72), V59 (= V74), D153 (= D158), N162 (= N170), V184 (≠ Q192), P192 (= P200)
binding : Y68 (≠ W83), L71 (≠ K86), V72 (≠ M87), V72 (≠ M87), S74 (≠ T89), F76 (≠ E91), F76 (≠ E91), Q80 (vs. gap)

Sites not aligning to the query:

8j0fA Gk tetramer with adjacent hooks at reaction state (see paper)
34% identity, 55% coverage: 17:200/332 of query aligns to 2:192/270 of 8j0fA

query
sites
8j0fA

D

D

R

R

A

S

-

R

A

A

L

F

Q

A

Q

K

R

D

A

V

R

K

R

R

W

I

V

V

I

V

K
|
K

I

V

G

G

S
x
T

S
x
A

L

V

L

V

T

T

Q

G

D

K

G

G

Q

G

R

R

L

L

D

A

L

L

S

G

A

R

M

L

Q

G

K

A

L

I

V

C

Q

E

Q

Q

I

L

L

A

R

E

L

L

R

N

A

S

E

D

D

G

I

F

E

E

V

V

V

I

L

L

V

V

S

S

S
|
S

G

G

S

A

V

V

S

G

A

L

G

G

K

R

C

Q

H

R

L

L

G

R

W

Y

A

R

Q

Q

K
x
L

M
x
V

T
x
N

T
x
S

A
x
S

E
x
F

-
x
A

-

D

-
x
L

-

Q

-

K

-

P

-

Q

-

M

-

E

-

L

E

D

R

G

Q

K

A

A

A

C

A

A

S

G

V

V

E

G

Q

Q

S

S

A

S

L

L

I

M

H

A

A

Y

Y

Y

E

E

N

T

L

M

L

F

A

D

E

Q

G

L

G

D

T

V

H

T

C

V

G

A

Q

Q

I

M

L

L

L

V

T

T

R

D

D

S

N

S

F

F

R

R

D

D

R

K

K

D

R

F

L

R

K

K

K

Q

T

L

R

S

S

E

A

T

I

V

E

K

M

A

L

M

L

L

R

R

M

M

Q

R

M

V

L

I

P

P

I

V

I

F

N

N

E

E

N

N

D
|
D

A

A

I

I

V

-

D

-

L

-

D

-

N

S

A

T

L

R

G

W

N

D

N
|
N

D

D

H

S

L

L

A

A

A

A

L

L

V

L

S

S

N

L

V

E

W

L

H

K

A

A

D

D

L

L

M

L

V

I

L

L

L

L

T

S

D
|
D

Q
x
V

S

E

G

G

L

L

F
x
Y

T

T

A
x
G

D

P

P

P

binding adenosine-5'-diphosphate: A21 (≠ S35), D183 (= D191), V184 (≠ Q192), Y188 (≠ F196), G190 (≠ A198)
binding gamma-glutamyl phosphate: K17 (= K31), T20 (≠ S34), S57 (= S71), D154 (= D158), N162 (= N170)
binding : L72 (≠ K86), V73 (≠ M87), N74 (≠ T88), S75 (≠ T89), S76 (≠ A90), F77 (≠ E91), F77 (≠ E91), A78 (vs. gap), L80 (vs. gap)

Sites not aligning to the query:

7f5xA Gk domain of drosophila p5cs filament with glutamate (see paper)
28% identity, 77% coverage: 23:276/332 of query aligns to 10:234/236 of 7f5xA

query
sites
7f5xA

Q

K

R

Y

A

A

R

R

R

R

W

L

V

V

I

V

K

K

I

L

G

G

S

S

S

A

L

V

L

I

T

T

-

R

Q

E

D

D

G

N

Q

H

R

G

L

L

D

A

L

L

S

G

A

R

M

L

Q

A

K

S

L

I

V

V

Q

E

Q

Q

I

V

L

A

R

E

L

C

R

H

A

L

E

E

D

G

I

R

E

E

V

V

V

M

L

M

V

V

S

T

S
|
S

G

G

S
x
A

V

V

S

A

A

F

G

G

K

K

C

Q

H

K

L

L

G

A

W

Q

A

E

Q

L

K

L

M

M

T

S

T

L

A

S

E

M

E

R

R

P

Q

R

A

A

A

A

A

A

S

A

V

V

E

G

Q

Q

S

S

A

G

L

L

I

M

H

S

A

L

Y

Y

E

D

N

A

L

M

L

F

A

A

E

Q

G

Y

G

G

T

V

H

K

C

I

G

A

Q

Q

I

V

L

L

L

V

T

T

R

K

D

P

N

D

F

F

R

Y

D

N

R

E

K

E

R

T

L

R

K

N

K

N

T

L

R

F

S

C

A

T

I

L

E

S

M

E

L

L

L

I

R

S

M

L

Q

N

M

I

L

V

P

P

I

I

I

I

N
|
N

E

T

N

N

D
|
D

A

A

I

V

V

S

D

P

L

-

D

-

N

-

A

-

L

-

G

-

N

D

N

N

D

D

H

S

L

L

A

S

A

A

L

M

V

L

S

A

N

A

V

E

W

V

H

Q

A

A

D

D

L

L

M

L

V

I

L

L

L

M

T

S

D

D

Q

V

S

D

G

G

L

I

F

Y

T

N

A

K

D

P

P

P

R

W

A

E

R

D

P

G

D

A

A

K

E

L

M

M

I

H

T

T

E

-

G

-

M

-

A

-

G

-

D

-

P

-

R

-

Y

-

E

-

H

-

M

-

A

-

G

-

G

-

S

-

G

-

G

-

T

-

V

-

G

-

T

-

G

-

G

-

M

Y

L

T

T

S

K

K

I

V

H

K

A

A

A

A

T

T

H

W

A

A

A

L

R

D

S

R

G

G

A

V

T

S

T

V

L

V

I

I

A

C

D

N

G

G

R

M

I

Q

P

E

D

K

V

A

L

I

L

K

R

T

L

I

H

I

K

G

G

G

A

R

L

K

L

V

G

G

T

T

F

F

L

F

binding gamma-l-glutamic acid: S59 (= S71), A61 (≠ S73), N143 (= N155), D146 (= D158)

7wx3B Gk domain of drosophila p5cs filament with glutamate, atp, and NADPH (see paper)
28% identity, 77% coverage: 23:276/332 of query aligns to 10:256/258 of 7wx3B

query
sites
7wx3B

Q

K

R

Y

A

A

R

R

R

R

W

L

V

V

I

V

K

K

I

L

G

G

S

S

S

A

L

V

L

I

T

T

-

R

Q

E

D

D

G

N

Q

H

R

G

L

L

D

A

L

L

S

G

A

R

M

L

Q

A

K

S

L

I

V

V

Q

E

Q

Q

I

V

L

A

R

E

L

C

R

H

A

L

E

E

D

G

I

R

E

E

V

V

V

M

L

M

V

V

S

T

S
|
S

G

G

S
x
A

V

V

S

A

A

F

G

G

K

K

C

Q

H

K

L

L

G

A

-

Q

-
x
E

-

L

-

L

-
x
M

-

S

W
x
L

A
x
S

Q
x
M

K
x
R

M

E

T

T

T

L

A

N

E

L

E

E

R

P

Q

R

A

A

A

A

A

A

S

A

V

V

E

G

Q

Q

S

S

A

G

L

L

I

M

H

S

A

L

Y

Y

E

D

N

A

L

M

L

F

A

A

E

Q

G

Y

G

G

T

V

H

K

C

I

G

A

Q

Q

I

V

L

L

L

V

T

T

R

K

D

P

N

D

F

F

R

Y

D

N

R

E

K

E

R

T

L

R

K

N

K

N

T

L

R

F

S

C

A

T

I

L

E

S

M

E

L

L

L

I

R

S

M

L

Q

N

M

I

L

V

P

P

I

I

I

I

N
|
N

E
x
T

N

N

D
|
D

A

A

I

V

V

S

-

P

D

P

L
x
M

D

F

N

I

A

P

L

I

G

K

N

D

N
|
N

D

D

H

S

L

L

A

S

A

A

L

M

V

L

S

A

N

A

V

E

W

V

H

Q

A

A

D

D

L

L

M

L

V

I

L

L

L

M

T

S

D

D

Q

V

S

D

G

G

L

I

F

Y

T

N

A

K

D

P

P

P

R

W

A

E

R

-

P

-

D

D

A

G

E

A

M

K

I

L

T

M

E

H

G

T

M

Y

A

T

G

S

D

D

P

D

R

S

Y

N

E

S

H

I

M

M

A

D

G

-

G

-

S

-

G

-

G

-

T

-

V

-

G

-

T

-

G

-

G

-

M

-

L

-

T

S

K

K

I

V

H

K

A

A

A

A

T

T

H

W

A

A

A

L

R

D

S

R

G

G

A

V

T

S

T

V

L

V

I

I

A

C

D

N

G

G

R

M

I

Q

P

E

D

K

V

A

L

I

L

K

R

T

L

I

H

I

K

G

G

G

A

R

L

K

L

V

G

G

T

T

F

F

L

F

binding gamma-glutamyl phosphate: S59 (= S71), A61 (≠ S73), N149 (= N155), T150 (≠ E156), D152 (= D158), M158 (≠ L163), N165 (= N170)
binding : E72 (vs. gap), M75 (vs. gap), L77 (≠ W83), S78 (≠ A84), M79 (≠ Q85), R80 (≠ K86)

2bmuB Ump kinase from pyrococcus furiosus complexed with its substrate ump and its substrate analog amppnp (see paper)
24% identity, 75% coverage: 25:274/332 of query aligns to 1:222/226 of 2bmuB

query
sites
2bmuB

A

A

R

M

R

R

W

I

V

V

I

F

K
x
D

I

I

G
|
G

S
x
G

S

S

L

V

L

L

T

V

Q

P

D

E

G

N

Q

P

R

D

L

I

D

D

L

F

S

-

A

-

M

I

Q

K

K

E

L

I

V

A

Q

Y

Q

Q

I

L

L

T

R

K

L

V

R

-

A

S

E

E

D

D

I

H

E

E

V

V

V

A

L

V

V

V

S

V

S
x
G

G
|
G

S
x
G

V

K

S

L

A

A

G

R

K

K

C

-

H

Y

L

I

G

E

W

V

A

A

Q

E

K

K

M

F

T

N

T

S

A

S

E

E

E

T

R

F

Q

K

A
x
D

A

F

A

I

S
x
G

V
x
I

E

Q

Q

-

S

-

A

-

L

I

I

T

H

R

A

A

Y

N

E

A

N

M

L

L

L

L

A

I

E

A

G

A

G

L

T

R

H

E

C

K

G

A

Q

Y

I

P

L

V

L

V

T

V

R

E

D

D

N

F

F

W

R

E

D

A

R

W

K

K

R

A

L

V

K

-

K

-

T

-

R

-

S

-

A

-

I

-

E

-

M

-

L

-

L

-

R

Q

M

L

Q

K

M

K

L

I

P

P

I

V

I

M

N

G

E
x
G

N
x
T

D

-

A

-

I

-

V

-

D

-

L

-

D

-

N
x
H

A

P

L

G

G
x
H

N
x
T

N
x
T

D
|
D

H

A

L

V

A

A

A

A

L

L

V

L

S

A

N

E

V

F

W

L

H

K

A

A

D

D

L

L

M

L

V

V

L

V

L

I

T
|
T

D
x
N

Q

V

S

D

G

G

L
x
V

F
x
Y

T

T

A
|
A

D
|
D

P
|
P

R

K

A

K

R

D

P

P

D

T

A

A

E

K

M

K

I

I

T

K

E

K

G

-

M

M

A

K

G

P

D

E

P

E

R

L

Y

L

E

E

H

-

M

I

A

V

G

G

G

K

S

G

G

I

G

E

T

K

V
x
A

G

G

T

S

G
x
S

G
x
S

M
x
V

L

I

T

D

K

P

I

L

H

-

A

A

A

A

T

K

H

I

A

I

A

A

R

R

S

S

G

G

A

I

T

K

T

T

L

I

I

V

A

I

D

G

G

K

R

E

I

D

P

A

D

K

V

D

L

L

L

F

R

R

L

V

H

I

K

K

G

G

A

D

L

H

L

N

G

G

T

T

binding phosphoaminophosphonic acid-adenylate ester: G9 (= G33), G10 (≠ S34), G44 (≠ S71), G45 (= G72), G46 (≠ S73), T141 (= T190), N142 (≠ D191), V146 (≠ L195), Y147 (≠ F196), A149 (= A198), D150 (= D199), P151 (= P200), S182 (≠ G233), S183 (≠ G234), V184 (≠ M235)
binding magnesium ion: D7 (≠ K31), D122 (= D171), D122 (= D171), S183 (≠ G234), V184 (≠ M235)
binding uridine-5'-monophosphate: G44 (≠ S71), G45 (= G72), D67 (≠ A95), G70 (≠ S98), I71 (≠ V99), G114 (≠ E156), T115 (≠ N157), H116 (≠ N165), H119 (≠ G168), T120 (≠ N169), T121 (≠ N170), A179 (≠ V230)

Q8U122 Uridylate kinase; UK; Uridine monophosphate kinase; UMP kinase; UMPK; EC 2.7.4.22 from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) (see paper)
24% identity, 75% coverage: 27:274/332 of query aligns to 2:221/225 of Q8U122

query
sites
Q8U122

R

R

W

I

V

V

I

F

K
x
D

I

I

G

G

S

G

S

S

L

V

L

L

T

V

Q

P

D

E

G

N

Q

P

R

D

L

I

D

D

L

F

S

-

A

-

M

I

Q

K

K

E

L

I

V

A

Q

Y

Q

Q

I

L

L

T

R

K

L

V

R

-

A

S

E

E

D

D

I

H

E

E

V

V

V

A

L

V

V

V

S

V

S

G

G
|
G

S

G

V

K

S

L

A

A

G

R

K

K

C

-

H

Y

L

I

G

E

W

V

A

A

Q

E

K

K

M

F

T

N

T

S

A

S

E

E

E

T

R

F

Q

K

A
x
D

A

F

A

I

S

G

V

I

E

Q

Q

-

S

-

A

-

L

I

I

T

H

R

A

A

Y

N

E

A

N

M

L

L

L

L

A

I

E

A

G

A

G

L

T

R

H

E

C

K

G

A

Q

Y

I

P

L

V

L

V

T

V

R

E

D

D

N

F

F

W

R

E

D

A

R

W

K

K

R

A

L

V

K

-

K

-

T

-

R

-

S

-

A

-

I

-

E

-

M

-

L

-

L

-

R

Q

M

L

Q

K

M

K

L

I

P

P

I

V

I

M

N

G

E

G

N
x
T

D

-

A

-

I

-

V

-

D

-

L

-

D

-

N
x
H

A
x
P

L
x
G

G
x
H

N
x
T

N
x
T

D
|
D

H

A

L

V

A

A

A

A

L

L

V

L

S

A

N

E

V

F

W

L

H

K

A

A

D

D

L

L

M

L

V

V

L

V

L

I

T

T

D

N

Q

V

S

D

G

G

L

V

F

Y

T

T

A

A

D

D

P

P

R

K

A

K

R

D

P

P

D

T

A

A

E

K

M

K

I

I

T

K

E

K

G

-

M

M

A

K

G

P

D

E

P

E

R

L

Y

L

E

E

H

-

M

I

A

V

G

G

G

K

S

G

G

I

G

E

T

K

V

A

G
|
G

T

S

G

S

G
x
S

M

V

L

I

T

D

K

P

I

L

H

-

A

A

A

A

T

K

H

I

A

I

A

A

R

R

S

S

G

G

A

I

T

K

T

T

L

I

I

V

A

I

D

G

G

K

R

E

I

D

P

A

D

K

V

D

L

L

L

F

R

R

L

V

H

I

K

K

G

G

A

D

L

H

L

N

G

G

T

T

D6 (≠ K31) binding Mg(2+)
G44 (= G72) binding UMP
D66 (≠ A95) binding UMP
114:120 (vs. 157:170, 0% identical) binding UMP
T120 (≠ N170) binding Mg(2+)
D121 (= D171) binding Mg(2+); binding Mg(2+)
G179 (= G231) binding UMP
S182 (≠ G234) binding Mg(2+)

Query Sequence

>WP_009566656.1 NCBI__GCF_000021485.1:WP_009566656.1
MTMNIVGKATMHSVVPDRAALQQRARRWVIKIGSSLLTQDGQRLDLSAMQKLVQQILRLR
AEDIEVVLVSSGSVSAGKCHLGWAQKMTTAEERQAAASVEQSALIHAYENLLAEGGTHCG
QILLTRDNFRDRKRLKKTRSAIEMLLRMQMLPIINENDAIVDLDNALGNNDHLAALVSNV
WHADLMVLLTDQSGLFTADPRARPDAEMITEGMAGDPRYEHMAGGSGGTVGTGGMLTKIH
AATHAARSGATTLIADGRIPDVLLRLHKGALLGTFLRSGKFDRKLRRDAWTRLQIHHGQQ
WVIHPVRRVKDEVVAFYSETPPFTAIPIFNGQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory