SitesBLAST

V59 (≠ I26) to L: in dbSNP:rs4008659
Y135 (≠ K105) Important for permeability to glycerol; mutation to A: Strongly decreased permeability to glycerol. Mildly decreased water permeability.
H165 (≠ N130) mutation to A: Decreased permeability to glycerol. Mildly decreased water permeability.
G264 (= G231) to V: affects water and glycerol transport; dbSNP:rs62542743

Sites not aligning to the query:

1:32 mutation Missing: Decreased interaction with PLIN1.
12 R → C: in dbSNP:rs139297434

8c9hA Aqp7_inhibitor (see paper)
34% identity, 98% coverage: 4:242/243 of query aligns to 12:250/253 of 8c9hA

query
sites
8c9hA

F

F

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L

A

A

E

E

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T

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Y

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-

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x
F

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A

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V

L

F

binding ethyl 4-[(4-pyrazol-1-ylphenyl)methylcarbamoylamino]benzoate: F49 (≠ W44), A66 (= A61), H67 (= H62), L157 (= L144), Q158 (≠ L145), F162 (= F149), I181 (≠ V174)

Q8II36 Aquaglyceroporin; PfAQP; Aquaporin-1 from Plasmodium falciparum (isolate 3D7) (see 2 papers)
33% identity, 98% coverage: 1:239/243 of query aligns to 10:238/258 of Q8II36

query
sites
Q8II36

M

V

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F

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M

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A

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D

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G

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A

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x
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x
F

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R

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G66 (= G60) binding glycerol
A67 (= A61) binding glycerol
N70 (= N64) binding glycerol
L71 (≠ P65) mutation to P: No significant effects on transport activity; when associated with A-131.
LA 71:72 (≠ PA 65:66) mutation to PS: Reduces stability and abolishes transport activity; when associated with 130-L-A-131.
S127 (≠ F131) binding glycerol
PS 130:131 (vs. gap) mutation to LA: Reduces stability and abolishes transport activity; when associated with 71-P-S-72.
S131 (vs. gap) mutation to A: No significant effects on transport activity; when associated with P-71.
G189 (= G190) binding glycerol
F190 (≠ Y191) binding glycerol
N193 (= N194) binding glycerol
R196 (= R197) binding glycerol

3c02A X-ray structure of the aquaglyceroporin from plasmodium falciparum (see paper)
34% identity, 98% coverage: 1:239/243 of query aligns to 3:231/242 of 3c02A

query
sites
3c02A

M

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A

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D

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x
F

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A

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R

D

D

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L

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R

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F

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L

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I

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A

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G

K

K

G

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K

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N

S

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Y

A

F

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W

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A

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L

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Y

binding glycerol: W43 (= W44), G182 (= G190), F183 (≠ Y191), R189 (= R197)

I1CR68 Aquaporin-1 from Rhizopus delemar (strain RA 99-880 / ATCC MYA-4621 / FGSC 9543 / NRRL 43880) (Mucormycosis agent) (Rhizopus arrhizus var. delemar) (see paper)
32% identity, 98% coverage: 4:240/243 of query aligns to 61:297/306 of I1CR68

query
sites
I1CR68

F

F

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G

A

A

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H

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L

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N

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P

A

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R

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N

A

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I

I

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M

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G

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A

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F

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N

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A

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A

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A

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-
x
H

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Y

Y

A

A

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M

L

M

H275 (vs. gap) mutation to A: Affects pH sensing; when associated with A-85.

6f7hC Crystal structure of human aqp10 (see paper)
32% identity, 98% coverage: 5:242/243 of query aligns to 10:248/253 of 6f7hC

query
sites
6f7hC

I

L

A

A

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A

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x
I

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A

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F

C

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T

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Y

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A

A

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P

Y

N

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N

N

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G

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L

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G

G

G

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N

P

P

A

A

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R

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D

L

L

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G

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R

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A

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A

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-

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K

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D

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S

N

G

G

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S

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Y

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A

-

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W

I

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P

I

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V

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G

A

P

P

I

L

I

V

G

G

A

A

V

T

L

V

A

G

A

T

L

A

L

T

F

Y

L

Q

V

L

L

L

binding glycerol: I55 (≠ V52), N199 (= N194)

Q96PS8 Aquaporin-10; AQP-10; Aquaglyceroporin-10; Small intestine aquaporin from Homo sapiens (Human) (see 2 papers)
32% identity, 98% coverage: 5:242/243 of query aligns to 25:263/301 of Q96PS8

query
sites
Q96PS8

I

L

A

A

E
|
E

V

F

L

L

G

G

T

V

A

F

L

V

L

L

I

M

L

L

G

T

G

Q

G

G

I

A

V

V

A

A

N

Q

D

A

I

V

L

T

N

S

-

G

K

E

T

T

K

K

G

G

N

N

G

-

W

F

M

F

T

T

I

M

T

F

T

L

A

A

W

G

G

S

L

L

A

A

V

V

F

T

V

I

G

A

V

I

V

Y

V

V

A
x
G

G

G

P

N

Y

V

S
|
S

G

G

A

A

H
|
H

L

L

N

N

P

P

A

A

V
x
F

T

S

I

L

G

A

L

M

A

C

A

I

G

V

G

G

L
x
R

F

L

P

P

W

W

A

V

D

K

V

L

P

P

T

I

Y

Y

L

I

A

L

A

V

E

Q

F

L

I

L

G

S

A

A

M

F

I

C

G

A

S

S

S

G

L

A

V

T

Y

Y

V

V

M

L

Y

Y

K

H

D

D

H

A

F

L

D

Q

A

N

T

Y

E

T

D

G

G

G

G
x
N

L

L

-

T

-

V

-

T

-

G

-

P

-

K

-

E

K

T

R

A

A

S

V

I

F

F

C

A

T

T

D

Y

P

P

A

A

I

P

P

Y

N

L

N

S

F

L

R

N

N

N

-

G

I

F

V

L

S

D

E

Q

I

V

L

L

G

G

T

T

F

G

V

M

L

L

L

I

I

V

C

G

V

L

F

L

Y

A

F

I

S

L

D

D

A

R

E

R

I

-

A

-

D

-

G

N

T

K

G

G

T

V

K

P

V

A

G

G

L

L

G

E

S

P

I

-

G

-

A

-

I

V

P

V

V

V

A

G

F

M

V

L

V

I

W

L

G

A

I

L

G

G

L

L

S

S

L

M

G

G

G

A

T

N

T

C

G

G

Y

I

A

P

I

L

N

N

P

P

A

A

R

R

D

D

L

L

G

G

P

P

R

R

I

L

V

F

H

T

A

Y

I

V

L

A

-

G

-

W

-

G

P

P

I

E

K

V

G

F

K

S

I

A

D

G

S

N

G

G

W

W

S

W

Y

-

A

-

W

W

I

V

P

P

I

V

V

V

G

A

P

P

I

L

I

V

G

G

A

A

V

T

L

V

A

G

A

T

L

A

L

T

F

Y

L

Q

V

L

L

L

E27 (= E7) mutation to Q: Abolishes permeability to glycerol.
G73 (≠ A55) mutation to A: Increased permeability to glycerol at acidic pH.; mutation to F: Abolishes permeability to glycerol.
S77 (= S59) mutation S->A,D: Nearly abolishes permeability to glycerol.
H80 (= H62) mutation to A: Abolishes permeability to glycerol.
F85 (≠ V67) mutation to A: Nearly abolishes permeability to glycerol.
R94 (≠ L76) mutation to A: Abolishes permeability to glycerol.
N133 (≠ G115) modified: carbohydrate, N-linked (GlcNAc...) asparagine; mutation to Q: Abolishes N-glycosylation.

8jy8A Structure of tbaqp2 in complex with anti-trypanosomatid drug pentamidine (see paper)
35% identity, 97% coverage: 4:239/243 of query aligns to 4:236/242 of 8jy8A

query
sites
8jy8A

F

Y

I

V

A

A

E

E

V

F

L

L

G

G

T

N

A

F

L

V

L

L

I

I

L

Y

L

I

G

A

G

K

G

G

I

A

V

V

A

I

N

T

D

S

I

L

L

L

N

V

K

P

T

D

K

F

G

G

N

-

G

-

W

L

M

L

T

G

I

L

T

T

T

I

A

G

W

I

G

G

L

V

A

A

V

V

F

T

V

M

G

A

V

L

V

Y

V

V

A

S

G

L

P

G

Y

I

S

S

G

G

A

G

H

H

L

L

N

N

P

S

A

A

V

V

T

T

I

V

G

G

L

N

A

A

A

V

G

F

G

G

L

D

F

F

P

P

W

W

A

R

D

K

V

V

P

P

T

G

Y

Y

L

I

A

A

E

Q

F

M

I

L

G

G

A

T

M

F

I

L

G

G

S

A

L

C

V

A

Y

Y

V

G

M

V

Y

F

K

A

D

D

H

L

F

L

D

K

A

A

T

H

E

G

D

G

G

G

G

E

L

L

K

I

R

A

A

F

-

G

-

E

-

K

-

G

-

I

-

A

-

W

V

V

F

F

C

A

T

M

D

Y

P

P

A

A

I

E

P

G

N

N

N

G

-

I

F

F

R

Y

N

P

I

I

V

F

S

A

E

E

I

L

L

I

G

S

T

T

F

A

V

V

L

L

I

L

C

C

V

V

F

C

Y

G

F

I

S

F

D

D

A

P

E

N

I

N

A

S

D

P

G

A

T

K

G

G

T

Y

K

E

V

T

G

-

L

-

G

V

S

A

I

I

G

G

A

A

I

L

P

-

V
|
V

A

F

F

V

V

M

V

V

W

N

G

N

I

F

G

G

L

L

S

A

L

-

G

-

T

-

T

S

G

P

Y

L

A
|
A

I

M

N
|
N

P

P

A

S

R
x
L

D

D

L

F

G

G

P

P

R

R

I

V

V

F

H

G

A

A

I

I

L

L

P

-

I

L

K

G

G

G

K

E

I

V

D

F

S

S

G

H

W

A

S

N

Y

Y

A

Y

-

F

W

W

I

V

P

P

I

L

V

V

G

V

P

P

I

F

G

G

A

A

V

I

L

L

A

G

A

L

L

F

L

L

F

Y

binding 1,5-bis(4-amidinophenoxy)pentane: V175 (= V174), A189 (= A192), N191 (= N194), L194 (≠ R197)

8jy6A Structure of tbaqp2 in complex with anti-trypanosomatid drug melarsoprol (see paper)
35% identity, 97% coverage: 4:239/243 of query aligns to 4:236/242 of 8jy6A

query
sites
8jy6A

F

Y

I

V

A

A

E

E

V

F

L

L

G

G

T

N

A

F

L

V

L

L

I

I

L

Y

L

I

G

A

G

K

G

G

I

A

V

V

A

I

N

T

D

S

I

L

L

L

N

V

K

P

T

D

K

F

G

G

N

-

G

-

W

L

M

L

T

G

I

L

T

T

T

I

A

G

W

I

G

G

L

V

A

A

V

V

F

T

V

M

G

A

V

L

V

Y

V

V

A

S

G

L

P

G

Y

I

S

S

G

G

A

G

H
|
H

L
|
L

N

N

P

S

A

A

V

V

T

T

I

V

G

G

L

N

A

A

A

V

G

F

G

G

L

D

F

F

P

P

W

W

A

R

D

K

V

V

P

P

T

G

Y

Y

L

I

A

A

E

Q

F

M

I

L

G

G

A

T

M

F

I

L

G

G

S

A

L

C

V

A

Y

Y

V

G

M

V

Y

F

K

A

D

D

H

L

F

L

D

K

A

A

T

H

E

G

D

G

G

G

G

E

L

L

K

I

R

A

A

F

-

G

-

E

-

K

-

G

-

I

-

A

-

W

V

V

F

F

C

A

T
x
M

D

Y

P

P

A

A

I

E

P

G

N

N

N

G

-

I

F

F

R

Y

N

P

I

I

V

F

S

A

E

E

I

L

L

I

G

S

T

T

F

A

V

V

L

L

I

L

C

C

V

V

F

C

Y

G

F

I

S

F

D

D

A

P

E

N

I

N

A

S

D

P

G

A

T

K

G

G

T

Y

K

E

V

T

G

-

L

-

G

V

S

A

I
|
I

G

G

A

A

I

L

P

-

V
|
V

A

F

F

V

V

M

V
|
V

W

N

G

N

I

F

G

G

L

L

S

A

L

-

G

-

T

-

T

S

G

P

Y

L

A
|
A

I

M

N
|
N

P

P

A

S

R
x
L

D

D

L

F

G

G

P

P

R

R

I

V

V

F

H

G

A

A

I

I

L

L

P

-

I

L

K

G

G

G

K

E

I

V

D

F

S

S

G

H

W

A

S

N

Y

Y

A

Y

-

F

W

W

I

V

P

P

I

L

V

V

G

V

P

P

I

F

G

G

A

A

V

I

L

L

A

G

A

L

L

F

L

L

F

Y

binding [(4~{R})-2-[4-[[4,6-bis(azanyl)-1,3,5-triazin-2-yl]amino]phenyl]-1,3,2-dithiarsolan-4-yl]methanol: H58 (= H62), L59 (= L63), M126 (≠ T123), I171 (= I169), V175 (= V174), V179 (= V178), A189 (= A192), N191 (= N194), L194 (≠ R197)

P0AER0 Glycerol uptake facilitator protein; Aquaglyceroporin; Glycerol facilitator from Escherichia coli (strain K12) (see 3 papers)
32% identity, 98% coverage: 5:242/243 of query aligns to 12:254/281 of P0AER0

query
sites
P0AER0

I

I

A

A

E

E

V

F

L

L

G

G

T

T

A

G

L

L

I

I

L

F

G

G

G

V

G

G

I

C

V

V

A

A

N

A

D

-

I

-

L

-

N

L

K

K

T

V

K

A

G

G

N

A

G

S

G

F

G

G

W

Q

M

W

T

E

I

I

T

S

T

V

A

I

W

W

G

G

L

L

A

G

V

V

F

A

V

M

G

A

V

I

V

Y

V

L

A

T

G

A

P

G

Y

V

S

S

G

G

A

A

H
|
H

L
|
L

N
|
N

P

P

A

A

V

V

T

T

I

I

G

A

L

L

A

W

A

L

G

F

G

A

L

C

F

F

P

D

W

K

A

R

D

K

V

V

P

I

T

P

Y

F

L

I

A

V

A

S

E

Q

F

V

I

A

G

G

A

A

M

F

I

C

G

A

S

A

L

L

V

V

Y

Y

V

G

M

L

Y

Y

K

Y

D

N

-

L

H

F

F

F

D

D

A

F

T

E

E

Q

D

T

G

H

L

I

K

V

R

R

-

G

-

S

-

V

-

E

-

S

-

V

-

D

-

L

-

A

A

G

V

T

F

F

C

S

T

T

D
x
Y

P

P

A

N

I

P

P

H

N

I

N

N

F

F

-

V

R

Q

N

A

I

F

V

A

S

V

E

E

I

M

L

V

G

I

T

T

F

A

V

I

L

L

L

M

I

G

C

L

V

I

F

L

Y

A

F

L

S

T

D

D

A

-

E

-

I

-

A

-

D

D

G

G

T

N

G

G

T

-

K

-

V

V

G

P

L

R

G

G

S

P

I

L

G

A

A

P

I

L

P

L

V

I

A

G

F

L

V

L

V

I

W

A

G

V

I

I

G

G

L

A

S

S

L

M

G

G

G

P

T

L

T

T

G
|
G

Y
x
F

A
|
A

I

M

N
|
N

P

P

A

A

R
|
R

D

D

L

F

G

G

P

P

R

K

I

V

V

F

H

A

A

W

I

L

L

-

P

-

I

-

K

-

G

-

K

-

I

-

D

-

S

A

G

G

W

W

-

G

-

N

-

V

-

A

-

F

-

T

-

G

-

R

-

D

-

I

S

P

Y

Y

A

F

W

L

I

V

P
|
P

I
x
L

V

F

G

G

P

P

I

I

I

V

G

G

A

A

V

I

L

V

A

G

A

A

L

F

L

A

F

Y

L

R

V

K

L

L

HLN 66:68 (= HLN 62:64) binding glycerol
Y138 (≠ D124) binding glycerol
GFA 199:201 (≠ GYA 190:192) binding glycerol
N203 (= N194) binding glycerol
R206 (= R197) binding glycerol
PL 236:237 (≠ PI 224:225) mutation to FW: No detectable water or glycerol permeability.

1fx8A Crystal structure of the e. Coli glycerol facilitator (glpf) with substrate glycerol (see paper)
34% identity, 98% coverage: 5:242/243 of query aligns to 7:249/254 of 1fx8A

query
sites
1fx8A

I

I

A

A

E

E

V

F

L

L

G

G

T

T

A

G

L

L

I

I

L

F

G

G

G

V

G

G

I

C

V

V

A

A

N

A

D

L

I

-

L

-

N

-

K

K

T

V

K

A

G

G

N

A

G

S

G

F

G

G

W

Q

M

W

T

E

I

I

T

S

T

V

A

I

W
|
W

G

G

L

L

A

G

V

V

F

A

V

M

G

A

V

I

V

Y

V

L

A

T

G

A

P

G

Y

V

S

S

G

G

A

A

H

H

L

L

N
|
N

P

P

A

A

V

V

T

T

I

I

G

A

L

L

A

W

A

L

G

F

G

A

L

C

F

F

P

D

W

K

A

R

D

K

V

V

P

I

T

P

Y

F

L

I

A

V

A

S

E

Q

F

V

I

A

G

G

A

A

M

F

I

C

G

A

S

A

L

L

V

V

Y

Y

V

G

M

L

Y

Y

K

Y

D

N

-

L

H

F

F

F

D

D

A

F

T

E

E

Q

D

T

G

H

L

I

K

V

R

R

-

G

-

S

-

V

-

E

-

S

-

V

-

D

-

L

-

A

A

G

V

T

F

F

C

S

T

T

D

Y

P

P

A

N

I

P

P

H

N

I

N

N

F

F

-

V

R

Q

N

A

I

F

V

A

S

V

E

E

I

M

L

V

G

I

T

T

F

A

V

I

L

L

L

M

I

G

C

L

V

I

F

L

Y

A

F

L

S

T

D

D

A

-

E

-

I

-

A

-

D

D

G

G

T

N

G

G

T

-

K

-

V

V

G

P

L

R

G

G

S

P

I

L

G

A

A

P

I

L

P

L

V

I

A

G

F

L

V

L

V

I

W

A

G

V

I

I

G

G

L

A

S

S

L

M

G

G

G

P

T

L

T

T

G
|
G

Y
x
F

A
|
A

I

M

N
|
N

P

P

A

A

R
|
R

D

D

L

F

G

G

P

P

R

K

I

-

V

V

H

F

A

A

I

W

L

L

P

A

I

G

K

W

G

G

K

N

I

V

D

A

-

F

-

T

-

G

S

R

G

D

W

I

S

P

Y

Y

A

F

W

L

I

V

P

P

I

L

V

F

G

G

P

P

I

I

I

V

G

G

A

A

V

I

L

V

A

G

A

A

L

F

L

A

F

Y

L

R

V

K

L

L

binding glycerol: W43 (= W44), N63 (= N64), G194 (= G190), F195 (≠ Y191), A196 (= A192), N198 (= N194), R201 (= R197)

P37451 Propanediol uptake facilitator PduF from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
33% identity, 96% coverage: 6:239/243 of query aligns to 11:249/264 of P37451

query
sites
P37451

A

A

E

E

V

F

L

L

G

G

T

T

A

G

L

L

L

F

I

L

L

F

G

G

G

I

G

G

I

C

V

L

A

S

N

A

D

L

I

-

L

-

N

-

K

K

T

V

K

A

G

G

N

A

G

S

G

L

G

G

W

L

M

W

T

E

I

I

T

C

T

I

A

I

W

W

G

G

L

L

A

G

V

I

F

S

V

L

G

A

V

V

V

Y

V

L

A

T

G

A

P

G

Y

I

S

S

G

G

A

G

H

H

L

L

N
|
N

P
|
P

A
|
A

V

V

T

T

I

I

G

A

L

L

A

W

A

L

G

F

G

A

L

C

F

F

P

P

W

K

A

Q

D

K

V

V

P

L

T

P

Y

Y

L

I

A

I

A

A

E

Q

F

F

I

A

G

G

A

A

M

F

I

G

G

G

S

A

S

L

L

L

V

A

Y

Y

V

V

M

L

Y

Y

K

S

D

S

H

L

F

F

D

T

A

E

T

F

E

E

-

T

-

A

-

H

-

M

-

V

D

R

G

G

G

S

L

V

K

E

R

S

-

L

-

Q

-

L

-

A

A

S

V

I

F

F

C

S

T

T

D

Y

P

P

A

A

I

A

P

A

-

L

N

N

N

V

F

W

R

Q

N

A

I

A

V

L

S

V

E

E

I

V

L

V

G

I

T

T

F

S

V

I

L

L

L

M

I

G

C

M

V

I

F

M

Y

A

F

L

S

T

D

D

A

-

E

-

I

-

A

-

D

D

G

G

T

N

G

G

T

-

K

-

V

I

G

P

L

K

G

G

S

P

I

L

G

A

A

P

I

L

P

L

V

I

A

G

F

I

V

L

V

V

W

A

G

V

I

I

G

G

L

A

S

S

L

T

G

G

G

P

T

L

T

T

G

G

Y

F

A

A

I

M

N
|
N

P
|
P

A
|
A

R

R

D

D

L

F

G

G

P

P

R

K

I

L

V

F

H

T

A

W

I

L

L

A

P

G

I

W

K

G

G

N

K

M

I

A

D

M

S

S

G

G

W

G

S

R

-

E

-

I

-

P

Y

Y

A

F

W

I

I

V

P

P

I

I

V

V

G

A

P

P

I

V

I

I

G

G

A

A

V

C

L

A

A

G

A

A

L

A

L

I

F

Y

NPA 66:68 (= NPA 64:66) NPA 1
NPA 201:203 (= NPA 194:196) NPA 2

B1VB61 Propanediol uptake facilitator PduF from Citrobacter freundii (see paper)
33% identity, 98% coverage: 5:242/243 of query aligns to 10:252/269 of B1VB61

query
sites
B1VB61

I

I

A

A

E

E

V

F

L

L

G

G

T

T

A

G

L

L

L

F

I

L

L

F

G

G

G

I

G

G

I

C

V

L

A

S

N

A

D

-

I

-

L

-

N

L

K

K

T

V

K

A

G

G

N

A

G

S

G

L

G

G

W

L

M

W

T

E

I

I

T

C

T

I

A

I

W

W

G

G

L

L

A

G

V

I

F

S

V

L

G

A

V

V

V

Y

V

L

A

T

G

A

P

G

Y

I

S

S

G

G

A

A

H

H

L

L

N
|
N

P
|
P

A
|
A

V

I

T

T

I

I

G

A

L

L

A

W

A

L

G

F

G

A

L

C

F

F

P

P

W

G

A

R

D

K

V

V

P

L

T

P

Y

Y

L

T

A

V

A

A

E

Q

F

V

I

A

G

G

A

A

M

F

I

G

G

G

S

A

S

L

L

L

V

A

Y

Y

V

L

M

L

Y

Y

K

G

D

S

H

L

F

F

D

T

A

E

T

Y

E

E

D

S

-

A

-

H

-

Q

-

M

-

V

-

R

G

G

G

S

L

L

K

E

R

S

-

L

-

H

-

L

-

A

A

S

V

I

F

F

C

S

T

T

D

Y

P

P

A

A

I

A

P

A

-

L

N

S

N

V

F

W

R

Q

N

A

I

A

V

L

S

V

E

E

I

V

L

V

G

I

T

T

F

S

V

I

L

L

L

M

I

G

C

M

V

I

F

M

Y

A

F

L

S

T

D

D

A

-

E

-

I

-

A

-

D

D

G

G

T

N

G

G

T

-

K

-

V

V

G

P

L

K

G

G

S

P

I

L

G

A

A

P

I

L

P

L

V

I

A

G

F

I

V

L

V

V

W

A

G

V

I

I

G

G

L

A

S

S

L

T

G

G

G

P

T

L

T

T

G

G

Y

F

A

A

I

M

N
|
N

P
|
P

A
|
A

R

R

D

D

L

F

G

G

P

P

R

K

I

L

V

F

H

A

A

W

I

M

-

A

-

G

-

W

-

G

-

D

L

V

P

A

I

M

K

T

G

G

K

G

I

R

D

D

S

I

G

-

W

-

S

P

Y

Y

A

F

W

I

I

V

P

P

I

I

V

V

G

A

P

P

I

I

G

G

A

A

V

C

L

A

A

G

A

A

L

A

L

I

F

Y

L

R

V

Y

L

L

NPA 66:68 (= NPA 64:66) NPA 1
NPA 201:203 (= NPA 194:196) NPA 2

P08995 Nodulin-26; N-26 from Glycine max (Soybean) (Glycine hispida) (see paper)
31% identity, 95% coverage: 1:232/243 of query aligns to 37:241/271 of P08995

query
sites
P08995

M

L

T

Q

P

K

F

L

I

V

A

A

E

E

V

A

L

V

G

G

T

T

A

Y

L

F

L

L

I

I

L

F

L

A

G

G

G

-

I

-

V

C

A

A

N

S

D

L

I

V

L

V

N

N

K

E

T

N

K

Y

G

Y

N

N

G

M

G

I

G

T

W

F

M

P

T

G

I

I

T

A

T

I

A

V

W

W

G

G

L

L

A

V

V

L

F

T

V

V

G

L

V

V

V

Y

V

T

A

V

G

G

P

H

Y

I

S

S

G

G

A

G

H

H

L

F

N

N

P

P

A

A

V

V

T

T

I

I

G

A

L

F

A

A

A

S

G

T

G

R

L

R

F

F

P

P

W

L

A

I

D

Q

V

V

P

P

T

A

Y

Y

L

V

A

V

A

A

E

Q

F

L

I

L

G

G

A

S

M

I

I

L

G

A

S

S

S

G

L

T

V

L

Y

R

V

L

M

L

Y

F

-

M

-

G

-

N

K

H

D

D

H

Q

F

F

D

S

A

G

T

T

E

-

D

-

G

-

L

-

K

-

R

-

A

-

V

-

F

-

C

-

T

-

D

-

P

-

A

-

I

V

P

P

N

N

-

G

-

T

N

N

F

L

R

Q

N

A

I

F

V

V

S

F

E

E

I

F

L

I

G

M

T

T

F

F

V

F

L

L

L

M

I

F

C

V

V

I

F

C

Y

G

F

V

S

A

D

T

A

D

E

N

I

R

A

A

D

V

G

G

T

E

G

F

T

A

K

G

V

I

G

A

L

I

G

G

S

S

I

T

G

L

A

L

I

L

P

N

V

V

A

I

F

-

V

-

W

-

G

-

I

I

G

G

L

-

S

-

L

-

G

G

G

P

T

V

T

T

G

G

Y

A

A

S

I

M

N

N

P

P

A

A

R

R

D

S

L

L

G

G

P

P

R

A

I

F

V

V

H

H

A

-

I

-

L

-

P

-

I

-

K

-

G

G

K

E

I

Y

D

E

S

G

G

I

W

W

S

I

Y

Y

A

L

W

L

I

A

P

P

I

V

V

V

G

G

P

A

I

I

I

A

G

G

A

A

Sites not aligning to the query:

262 modified: Phosphoserine; by CPK

2evuA Crystal structure of aquaporin aqpm at 2.3a resolution (see paper)
32% identity, 98% coverage: 5:243/243 of query aligns to 9:244/245 of 2evuA

query
sites
2evuA

I

I

A

A

E

E

V

F

L

I

G

G

T

T

A

F

L

I

L

L

I

V

L

F

G

G

G

A

G

G

I

S

V

A

A

A

N

V

D

T

I

L

L

M

N

I

K

A

T

S

K

G

G

G

N

T

G

S

-

P

-

N

-

P

-

F

-

N

-
x
I

-

G

-

I

-

G

-

L

-

G

-

L

G

G

G

D

W
|
W

M

V

T

A

I

I

T

G

T

L

A

A

W

F

G

G

L

F

A

A

V
x
I

F

A

V

A

G

S

V

I

V

Y

V

A

A

L

G

G

P

N

Y

I

S

S

G

G

A

C

H

H

L
x
I

N
|
N

P

P

A

A

V

V

T

T

I

I

G

G

L

L

A

W

A

S

G

V

G

K

L

K

F

F

P

P

W

G

A

R

D

E

V

V

P

V

T

P

Y

Y

L

I

A

I

A

A

E

Q

F

L

I

L

G

G

A

A

M

A

I

F

G

G

S

-

S

S

L

F

V

I

Y

F

V

L

M

Q

Y

C

K

A

D

G

H

I

F

G

D

A

A

A

T

T

E

V

D

G

G

G

G

L

L
x
G

K
x
A

R

T

A
|
A

V
x
P

F
|
F

C

-

T

-

D

-

P

P

A

G

I

I

P

-

N

S

N

Y

F

W

R

Q

N

A

I

M

V

L

S

A

E

E

I

V

L

V

G

G

T

T

F

F

V

L

L

L

L

M

I

I

C

T

V

I

F

M

Y

G

F

I

S

A

D

V

A

D

E

E

I

R

A

A

D

P

G

-

T

-

G

-

T

-

K

-

V

-

G

-

L

-

G

K

S

G

I

F

G

A

A

G

I

I

P

I

V

I

A

G

F

L

V

T

V

V

W

A

G

G

I

I

G

I

L

T

S

T

L

L

G

G

G

N

T

I

T

S

G

G

Y

S

A

S

I

L

N

N

P

P

A

A

R

R

D

T

L

F

G

G

P

P

R

Y

I

L

V

N

H

D

A

M

I

I

L

F

P

A

I

G

K

T

G

D

K

L

I

-

D

-

S

-

G

-

W

W

S

N

Y

Y

A

Y

W

S

I

I

P

Y

I

V

V

I

G

G

P

P

I

I

I

V

G

G

A

A

V

V

L

L

A

A

L

L

L

T

F

Y

L

Q

V

Y

L

L

N

T

binding glycerol: I44 (vs. gap), W55 (= W37), I66 (≠ V48), I81 (≠ L63), N82 (= N64), G133 (≠ L116), A134 (≠ K117), A136 (= A119), P137 (≠ V120), F138 (= F121)

P61837 Aquaporin PIP1-1; AtPIP1;1; Plasma membrane aquaporin-1; Plasma membrane intrinsic protein 1a; PIP1a from Arabidopsis thaliana (Mouse-ear cress) (see paper)
32% identity, 84% coverage: 40:242/243 of query aligns to 90:276/286 of P61837

query
sites
P61837

I

I

T

A

T

W

A

A

W

F

G

G

L

G

A

M

V

I

F

F

V

A

G

L

V

V

V

Y

V

C

A

T

G

A

P

G

Y

I

S

S

G

G

A

G

H

H

L

I

N

N

P

P

A

A

V

V

T

T

I

F

G

G

L

L

A

F

A

L

G

A

G

R

L

K

F

L

P

S

W

L

A

T

D

R

V

A

P

L

T

Y

Y

Y

L

I

A

V

A

M

E

Q

F

C

I

L

G

G

A

A

M

I

I

C

G

G

S

A

S

G

L

V

V

V

Y

K

V

G

M

F

Y

Q

K

P

D

K

H

Q

F

Y

D

Q

A

A

T

L

E

G

D

G

G

G

G

A

L

N

K

T

R

V

A

A

V

H

F

G

C

Y

T

T

D

K

P

G

A

S

I

-

P

-

N

-

F

-

R

-

N

G

I

L

V

G

S

A

E

E

I

I

L

I

G

G

T

T

F

F

V

V

L

L

L

V

I

Y

C

T

V

V

F

F

Y

S

F

A

S

T

D

D

A

A

E

K

I

-

A

R

D

N

G

A

T

R

G

D

T

S

K

H

V

V

G

P

L

I

G

-

S

-

I

L

G

A

A

P

I

L

P

P

V

I

A

G

F

F

V

A

V

V

W

F

G

L

I

V

G

H

L

L

S

A

L

T

G

I

G

P

T

I

T

T

G

G

Y

T

A

G

I

I

N

N

P

P

A

A

R

R

D

S

L

L

G

G

P

A

R

A

I

I

V

I

H

Y

A

-

I

-

L

-

P

-

I

-

K

-

G

-

K

N

I

K

D

D

S

H

G

S

W

W

S

D

Y

D

A

H

W

W

I

V

P

F

I

W

V

V

G

G

P

P

I

F

I

I

G

G

A

A

V

A

L

L

A

A

L

L

L

Y

F

H

L

V

V

V

L

V

Sites not aligning to the query:

1 modified: N-acetylmethionine

Q06611 Aquaporin PIP1-2; AtPIP1;2; Plasma membrane intrinsic protein 1b; PIP1b; Transmembrane protein A; AthH2; TMP-A from Arabidopsis thaliana (Mouse-ear cress) (see paper)
31% identity, 84% coverage: 40:242/243 of query aligns to 90:276/286 of Q06611

query
sites
Q06611

I

I

T

A

T

W

A

A

W

F

G

G

L

G

A

M

V

I

F

F

V

A

G

L

V

V

V

Y

V

C

A

T

G

A

P

G

Y

I

S

S

G

G

A

G

H

H

L

I

N

N

P

P

A

A

V

V

T

T

I

F

G

G

L

L

A

F

A

L

G

A

G

R

L

K

F

L

P

S

W

L

A

T

D

R

V

A

P

V

T

Y

Y

Y

L

I

A

V

A

M

E

Q

F

C

I

L

G

G

A

A

M

I

I

C

G

G

S

A

S

G

L

V

V

V

Y

K

V

G

M

F

Y

Q

K

P

D

K

H

Q

F

Y

D

Q

A

A

T

L

E

G

D

G

G

G

G

A

L

N

K

T

R

I

A

A

V

H

F

G

C

Y

T

T

D

K

P

G

A

S

I

-

P

-

N

-

F

-

R

-

N

G

I

L

V

G

S

A

E

E

I

I

L

I

G

G

T

T

F

F

V

V

L

L

L

V

I

Y

C

T

V

V

F

F

Y

S

F

A

S

T

D

D

A

A

E

K

I

-

A

R

D

N

G

A

T

R

G

D

T

S

K

H

V

V

G

P

L

I

G

-

S

-

I

L

G

A

A

P

I

L

P

P

V

I

A

G

F

F

V

A

V

V

W

F

G

L

I

V

G

H

L

L

S

A

L

T

G

I

G

P

T

I

T

T

G

G

Y

T

A

G

I

I

N

N

P

P

A

A

R

R

D

S

L

L

G

G

P

A

R

A

I

I

V

I

H

F

A

-

I

-

L

-

P

-

I

-

K

-

G

-

K

N

I

K

D

D

S

N

G

A

W

W

S

D

Y

D

A

H

W

W

I

V

P

F

I

W

V

V

G

G

P

P

I

F

I

I

G

G

A

A

V

A

L

L

A

A

L

L

L

Y

F

H

L

V

V

I

L

V

Sites not aligning to the query:

1 modified: N-acetylmethionine

Q6Z2T3 Aquaporin NIP2-1; Low silicon protein 1; NOD26-like intrinsic protein 2-1; OsNIP2;1; Silicon influx transporter LSI1 from Oryza sativa subsp. japonica (Rice) (see paper)
29% identity, 94% coverage: 5:232/243 of query aligns to 52:251/298 of Q6Z2T3

query
sites
Q6Z2T3

I

V

A

S

E

E

V

V

L

V

G

A

T

T

A

F

L

L

I

V

L

F

L

M

-

T

-

C

-

G

G

A

G

G

I

I

V

S

A

G

N

S

D

D

I

L

L

S

N

R

K

I

T

S

K

Q

G

-

N

-

G

-

W

-

M

L

T

G

I

Q

T

S

T

I

A

A

W

G

G

G

L

L

A

I

V

V

F

T

V

V

G

M

V

I

V

Y

V

A

A

V

G

G

P

H

Y

I

S

S

G

G

A

A

H

H

L

M

N

N

P

P

A

A

V

V

T

T

I

L

G

A

L

F

A

A

A

V

G

F

G

R

L

H

F

F

P

P

W

W

A

I

D

Q

V

V

P

P

T

F

Y

Y

L

W

A

A

A
|
A

E

Q

F

F

I

T

G

G

A

A

M

I

I

C

G

A

S

S

S

F

L

V

V

L

Y

K

V

A

M

V

Y

I

K

H

D

P

H

-

F

-

D

-

A

-

T

-

E

-

D

-

G

-

L

-

K

V

R

D

A

V

V

I

F

G

C

T

T

T

D

T

P

P

A

V

I

G

P

P

N

-

N

H

F

W

R

H

N

S

I

L

V

V

S

V

E

E

I

V

L

I

G

V

T

T

F

F

V

N

L

M

I

F

C

V

V

T

F

-

Y

-

F

-

S

-

D

-

A

-

E

-

I

L

A

A

D

V

G

A

T

T

G

D

T

T

K

R

V

-

G

A

L

V

G

G

S

E

I

L

G

A

A

G

I

L

P

A

V

V

A

G

F

S

V

A

V

V

W

C

G

I

I

T

G

S

L

I

S

F

L

A

G

G

G

A

T

I

T

S

G

G

Y

G

A

S

I

M

N

N

P

P

A

A

R

R

D

T

L

L

G

G

P

P

R

A

I

L

V

A

H

-

A

-

I

-

L

-

P

-

I

-

K

S

G

N

K

K

I

F

D

D

S

G

G

L

W

W

S

I

Y

Y

A

F

W

L

I

G

P

P

I

V

V

M

G

G

P

T

I

L

I

S

G

G

A

A

A132 (= A88) mutation to T: In lsi; impairs silicon uptake. Grain discoloration. Reduces grain yield 10-fold.

4nefA X-ray structure of human aquaporin 2 (see paper)
31% identity, 83% coverage: 38:239/243 of query aligns to 41:218/239 of 4nefA

query
sites
4nefA

M

L

T

Q

I

I

T

A

T

M

A

A

W

F

G

G

L

L

A

G

V

I

F

G

V

T

G

L

V

V

V

Q

V

A

A

L

G

G

P

H

Y

I

S

S

G

G

A

A

H

H

L

I

N

N

P

P

A

A

V

V

T

T

I

V

G

A

L
x
C

A

L

A

V

G

G

G

C

L
x
H

F

V

P

S

W

V

A

L

D

R

V

A

P

A

T

F

Y

Y

L

V

A

A

E

Q

F

L

I

L

G

G

A

A

M

V

I

A

G

G

S

A

L

L

V

L

Y

H

V

E

M

I

Y

T

K

P

D

A

H

-