SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_009779357.1 NCBI__GCF_000152985.1:WP_009779357.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q02635 Aspartate/prephenate aminotransferase; AspAT / PAT; Transaminase A; EC 2.6.1.1; EC 2.6.1.79 from Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) (see paper)
49% identity, 97% coverage: 8:395/398 of query aligns to 4:394/400 of Q02635

query
sites
Q02635

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K12 (≠ A16) Important for prephenate aminotransferase activity; mutation to G: 7-fold increase in Km for prephenate. Weakly affects Km for oxaloacetate.

6f77A Crystal structure of the prephenate aminotransferase from rhizobium meliloti (see paper)
49% identity, 97% coverage: 8:395/398 of query aligns to 3:393/399 of 6f77A

query
sites
6f77A

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binding pyridoxal-5'-phosphate: G98 (= G103), G99 (≠ A104), K100 (= K105), W124 (= W129), N170 (≠ S175), N174 (= N179), D203 (= D208), Y206 (= Y211), S237 (≠ A240), K238 (= K241), R246 (= R249)

5wmhA Arabidopsis thaliana prephenate aminotransferase (see paper)
45% identity, 98% coverage: 8:397/398 of query aligns to 2:396/399 of 5wmhA

query
sites
5wmhA

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binding pyridoxal-5'-phosphate: G97 (= G103), A98 (= A104), K99 (= K105), W123 (= W129), N173 (= N179), D202 (= D208), I204 (= I210), Y205 (= Y211), S235 (≠ A240), K236 (= K241), R244 (= R249)

5wmlA Arabidopsis thaliana prephenate aminotransferase mutant- k306a (see paper)
45% identity, 98% coverage: 8:397/398 of query aligns to 3:397/404 of 5wmlA

query
sites
5wmlA

L

L

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E

L

L

K

R

D

E

A

A

I

I

I

C

T

R

K

K

F

L

K

K

R

E

D

E

N

N

N

G

L

L

T

S

Y

Y

D

A

R

P

S

D

Q

Q

I

I

V

L

V

V

S

S

T

N

G
|
G

A
|
A

K
|
K

Q

Q

S

S

L
|
L

Y

L

N

Q

V

A

A

V

Q

L

V

A

C

V

L

C

N

S

P

P

G

G

D

D

E

E

V

V

L

I

L

I

P

P

C

A

P

P

Y

Y

W
|
W

V

V

S

S

Y

Y

S

T

D

E

I

Q

V

A

K

R

L

L

A

A

E

D

G

A

V

T

P

P

V

V

E

V

V

I

E

P

T

T

S

K

L

I

D

S

T

N

D

N

F

F

K

L

M

L

T

D

P

P

E

K

Q

D

L

L

E

E

A

S

A

K

I

L

T

T

P

E

K

K

T

S

K

R

M

L

L

L

W

I

Y

L

S

C

S

S

P

P

C

S

N
|
N

P

P

S

T

G

G

S

S

I

V

Y

Y

S

P

E

K

A

S

E

L

L

L

R

E

A

E

L

I

A

A

D

R

V

I

L

I

Q

A

K

K

H

H

P

P

Q

R

I

L

V

L

V

V

V

L

S

S

D
|
D

E

E

I

I

Y
|
Y

E

E

H

H

I

I

N

I

Y

Y

V

A

-

P

G

A

G

T

H

H

A

T

S

S

M

F

A

A

Q

S

F

L

E

P

D

D

M

M

Y

Y

D

E

R

R

V

T

V

L

T

T

V

V

N

N

G

G

V

F

A
x
S

K

A

A

A

F

F

A

A

M

M

T

T

G

G

W

W

R
|
R

I

L

G

G

Y

Y

I

L

G

A

A

G

P

P

A

K

Y

H

I

I

A

V

R

A

A

A

C

C

N

S

K

K

I

L

Q

Q

G

G

Q

Q

V

V

T

S

S

S

G

G

A

A

N

S

C

S

I

I

A

A

Q

Q

R

K

A

A

V

G

I

V

T

A

A

A

L

L

-

G

-

L

-

G

E

K

A

A

P

G

V

G

S

E

K

T

I

V

Q

A

Y

E

M

M

V

V

D

K

E

A

F

Y

K

R

E

E

R

R

R

R

K

D

L

F

I

L

L

V

G

K

L

S

L

L

N

G

D

D

I

I

E

K

G

G

F

V

E

K

C

I

N

S

E

E

P

P

E

Q

G

G

A

A

F

F

Y
|
Y

V

L

F

F

P

I

N

D

I

F

S

S

H

A

Y

Y

F

Y

G

G

K

S

T

E

L

A

N

E

G

G

-

F

T

G

T

L

I

I

N

N

N

D

A

S

S

S

D

S

F

L

A

A

L

L

Y

Y

I

F

L

L

E

D

Q

K

A

F

N

Q

V

V

A

A

T

M

V

V

T

P

G

G

E

D

A

A

F

F

G

G

N

D

P

D

N

S

C

C

I

I

R
|
R

I

I

S

S

Y

Y

A

A

A

T

S

S

Q

L

D

D

Q

V

I

L

K

Q

E

A

A

A

L

V

A

E

R

K

I

I

K

R

K

K

A

A

V

L

binding glutamic acid: T15 (= T20), M16 (≠ L21), A37 (≠ L42), G38 (= G43), K100 (= K105), W124 (= W129), Y326 (= Y327), R376 (= R376)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G98 (= G103), A99 (= A104), K100 (= K105), L103 (= L108), W124 (= W129), N174 (= N179), D203 (= D208), Y206 (= Y211), S236 (≠ A240), R245 (= R249)

5wmiA Arabidopsis thaliana prephenate aminotransferase mutant- t84v (see paper)
45% identity, 98% coverage: 8:397/398 of query aligns to 3:396/402 of 5wmiA

query
sites
5wmiA

L

L

S

S

D

P

R

R

I

V

N

Q

N

S

L

L

A

K

A

P

S

S

A

K

T
x
V

L

M

A

V

M

I

A

T

A

D

K

L

A

A

R

A

E

T

L

L

R

-

A

V

E

S

G

G

K

V

D

P

I

V

I

I

G

R

L

L

S

A

L
x
A

G

G

E

E

P

P

D

D

F

F

N

D

T

T

P

P

D

K

F

V

I

V

K

A

D

E

A

A

A

G

I

I

Q

N

A

A

V

I

N

R

D

E

N

G

Y

F

N

T

S

R

Y

Y

T

T

P

L

V

N

D

A

G

G

Y

I

V

T

E

E

L

L

K

R

D

E

A

A

I

I

I

C

T

R

K

K

F

L

K

K

R

E

D

E

N

N

N

G

L

L

T

S

Y

Y

D

A

R

P

S

D

Q

Q

I

I

V

L

V

V

S

S

T

N

G

G

A

A

K

K

Q

Q

S

S

L

L

Y

L

N

Q

V

A

A

V

Q

L

V

A

C

V

L

C

N

S

P

P

G

G

D

D

E

E

V

V

L

I

L

I

P

P

C

A

P

P

Y

Y

W

W

V

V

S

S

Y

Y

S

T

D

E

I

Q

V

A

K

R

L

L

A

A

E

D

G

A

V

T

P

P

V

V

E

V

V

I

E

P

T

T

S

K

L

I

D

S

T

N

D

N

F

F

K

L

M

L

T

D

P

P

E

K

Q

D

L

L

E

E

A

S

A

K

I

L

T

T

P

E

K

K

T

S

K

R

M

L

L

L

W

I

Y

L

S

C

S

S

P

P

C

S

N
|
N

P

P

S

T

G

G

S

S

I

V

Y

Y

S

P

E

K

A

S

E

L

L

L

R

E

A

E

L

I

A

A

D

R

V

I

L

I

Q

A

K

K

H

H

P

P

Q

R

I

L

V

L

V

V

V

L

S

S

D

D

E

E

I

I

Y

Y

E

E

H

H

I

I

N

I

Y

Y

V

A

-

P

G

A

G

T

H

H

A

T

S

S

M

F

A

A

Q

S

F

L

E

P

D

D

M

M

Y

Y

D

E

R

R

V

T

V

L

T

T

V

V

N

N

G

G

V

F

A

S

K
|
K

A

A

F

F

A

A

M

M

T

T

G

G

W

W

R

R

I

L

G

G

Y

Y

I

L

G

A

A

G

P

P

A

K

Y

H

I

I

A

V

R

A

A

A

C

C

N

S

K

K

I

L

Q

Q

G

G

Q

Q

V

V

T

S

S

S

G

G

A

A

N

S

C

S

I

I

A

A

Q

Q

R

K

A

A

V

G

I

V

T

A

A

A

L

L

-

G

-

L

-

G

E

K

A

A

P

G

V

G

S

E

K

T

I

V

Q

A

Y

E

M

M

V

V

D

K

E

A

F

Y

K

R

E

E

R

R

R

R

K

D

L

F

I

L

L

V

G

K

L

S

L

L

N

G

D

D

I

I

E

K

G

G

F

V

E

K

C

I

N

S

E

E

P

P

E

Q

G

G

A

A

F

F

Y

Y

V

L

F

F

P

I

N

D

I

F

S

S

H

A

Y

Y

F

Y

G

G

K

S

T

E

L

A

N

E

G

G

-

F

T

G

T

L

I

I

N

N

N

D

A

S

S

S

D

S

F

L

A

A

L

L

Y

Y

I

F

L

L

E

D

Q

K

A

F

N

Q

V

V

A

A

T

M

V

V

T

P

G

G

E

D

A

A

F

F

G

G

N

D

P

D

N

S

C

C

I

I

R
|
R

I

I

S

S

Y

Y

A

A

A

T

S

S

Q

L

D

D

Q

V

I

L

K

Q

E

A

A

A

L

V

A

E

R

K

I

I

K

R

K

K

A

A

V

L

binding 2-oxoglutaric acid: V15 (≠ T20), A36 (≠ L42), N173 (= N179), K236 (= K241), R375 (= R376)

P58350 Aspartate aminotransferase; AAT; AspAT; Putative 2-aminoadipate transaminase; Transaminase A; EC 2.6.1.1; EC 2.6.1.39 from Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) (see paper)
46% identity, 98% coverage: 7:396/398 of query aligns to 13:405/410 of P58350

query
sites
P58350

Q

Q

L

P

S

A

D

S

R

R

I

I

N

S

N

S

L

I

A

G

A

V

S

S

A

E

T

I

L

L

A

K

M

I

A

G

A

A

K

R

A

A

R

A

E

A

L

M

R

K

A

R

E

E

G

G

K

K

D

P

I

V

I

I

G

I

L

L

S

G

L

A

G

G

E

E

P

P

D

D

F

F

N

D

T

T

P

P

D

E

F

H

I

V

K

K

D

Q

A

A

A

A

I

S

Q

D

A

A

V

I

N

H

D

R

N

G

Y

E

N

T

S

K

Y

Y

T

T

P

A

V

L

D

D

G

G

Y

T

V

P

E

E

L

L

K

K

D

K

A

A

I

I

I

R

T

E

K

K

F

F

K

Q

R

R

D

E

N

N

N

G

L

L

T

A

Y

Y

D

E

R

L

S

D

Q

E

I

I

V

T

V

V

S

A

T

T

G

G

A

A

K

K

Q

Q

S

I

L

L

Y

F

N

N

V

A

A

M

Q

M

V

A

C

S

L

L

N

D

P

P

G

G

D

D

E

E

V

V

L

I

L

I

P

P

C

T

P

P

Y

Y

W

W

V

T

S

S

Y

Y

S

S

D

D

I

I

V

V

K

H

L

I

A

C

E

E

G

G

V

K

P

P

V

V

E

L

V

I

E

A

T

C

S

D

L

A

D

S

T

S

D

G

F

F

K

R

M

L

T

T

P

A

E

E

Q

K

L

L

E

E

A

A

A

A

I

I

T

T

P

P

K

R

T

T

K

R

M

W

L

V

W

L

Y

L

S

N

S

S

P

P

C

S

N

N

P

P

S

S

G

G

S

A

I

A

Y

Y

S

S

E

A

A

A

E

D

L

Y

R

R

A

P

L

L

A

L

D

E

V

V

L

L

Q

L

K

R

H

H

P

P

Q

H

I

V

V

W

V

L

V

L

S

V

D

D

E

D

I

M

Y

Y

E

E

H

H

I

I

N

V

Y

Y

V

D

G

G

G

F

H

R

-

F

A

V

S

T

M

P

A

A

Q

Q

F

L

E

E

-

P

D

G

M

L

Y

K

D

N

R

R

V

T

V

L

T

T

V

V

N

N

G

G

V

V

A

S

K
|
K

A

A

F

Y

A

A

M

M

T

T

G

G

W

W

R

R

I

I

G

G

Y

Y

I

A

G

G

A

G

P

P

A

R

Y

E

I

L

A

I

R

K

A

A

C

M

N

A

K

V

I

V

Q

Q

G

S

Q

Q

V

A

T

T

S

S

G

C

A

P

N

S

C

S

I

I

A

S

Q

Q

R

A

A

A

V

S

I

V

T

A

A

A

L

L

E

N

A

G

P

P

V

Q

S

D

K

F

I

L

Q

K

Y

E

M

R

V

T

D

E

E

S

F

F

K

Q

E

R

R

R

R

R

K

D

L

L

I

V

L

V

G

N

L

G

L

L

N

N

D

A

I

I

E

D

G

G

F

L

E

D

C

C

N

R

E

V

P

P

E

E

G

G

A

A

F

F

Y

Y

V

T

F

F

P

S

N

G

I

C

S

A

H

G

Y

V

F

L

G

G

K

K

-

V

T

T

L

P

N

S

G

G

T

K

T

R

I

I

N

K

N

T

A

D

S

T

D

D

F

F

A

C

L

A

Y

Y

I

L

L

L

E

E

Q

D

A

A

N

H

V

V

A

A

T

V

V

V

T

P

G

G

E

S

A

A

F

F

G

G

N

L

P

S

N

P

C

F

I

F

R

R

I

I

S

S

Y

Y

A

A

A

T

S

S

Q

E

D

A

Q

E

I

L

K

K

E

E

A

A

L

L

A

E

R

R

I

I

K

A

K

A

A

A

K249 (= K241) modified: N6-(pyridoxal phosphate)lysine

6f35A Crystal structure of the aspartate aminotranferase from rhizobium meliloti (see paper)
46% identity, 98% coverage: 7:396/398 of query aligns to 3:395/400 of 6f35A

query
sites
6f35A

Q

Q

L

P

S

A

D

S

R

R

I

I

N

S

N

S

L

I

A

G

A

V

S

S

A

E

T

I

L

L

A

K

M

I

A

G

A

A

K

R

A

A

R

A

E

A

L

M

R

K

A

R

E

E

G

G

K

K

D

P

I

V

I

I

G

I

L

L

S

G

L

A

G

G

E

E

P

P

D

D

F

F

N

D

T

T

P

P

D

E

F

H

I

V

K

K

D

Q

A

A

A

A

I

S

Q

D

A

A

V

I

N

H

D

R

N

G

Y

E

N

T

S

K

Y

Y

T

T

P

A

V

L

D

D

G

G

Y

T

V

P

E

E

L

L

K

K

D

K

A

A

I

I

I

R

T

E

K

K

F

F

K

Q

R

R

D

E

N

N

N

G

L

L

T

A

Y

Y

D

E

R

L

S

D

Q

E

I

I

V

T

V

V

S

A

T

T

G
|
G

A
|
A

K
|
K

Q

Q

S

I

L

L

Y

F

N

N

V

A

A

M

Q

M

V

A

C

S

L

L

N

D

P

P

G

G

D

D

E

E

V

V

L

I

L

I

P

P

C

T

P

P

Y

Y

W

W

V

T

S

S

Y

Y

S

S

D

D

I

I

V

V

K

H

L

I

A

C

E

E

G

G

V

K

P

P

V

V

E

L

V

I

E

A

T

C

S

D

L

A

D

S

T

S

D

G

F

F

K

R

M

L

T

T

P

A

E

E

Q

K

L

L

E

E

A

A

A

A

I

I

T

T

P

P

K

R

T

T

K

R

M

W

L

V

W

L

Y

L

S

N

S

S

P

P

C

S

N

N

P

P

S

S

G

G

S

A

I

A

Y

Y

S

S

E

A

A

A

E

D

L

Y

R

R

A

P

L

L

A

L

D

E

V

V

L

L

Q

L

K

R

H

H

P

P

Q

H

I

V

V

W

V

L

V

L

S

V

D
|
D

E

D

I
x
M

Y
|
Y

E

E

H

H

I

I

N

V

Y

Y

V

D

G

G

G

F

H

R

-

F

A

V

S

T

M

P

A

A

Q

Q

F

L

E

E

-

P

D

G

M

L

Y

K

D

N

R

R

V

T

V

L

T

T

V

V

N

N

G

G

V

V

A
x
S

K
|
K

A

A

F

Y

A

A

M

M

T

T

G

G

W

W

R
|
R

I

I

G

G

Y

Y

I

A

G

G

A

G

P

P

A

R

Y

E

I

L

A

I

R

K

A

A

C

M

N

A

K

V

I

V

Q

Q

G

S

Q

Q

V

A

T

T

S

S

G

C

A

P

N

S

C

S

I

I

A

S

Q

Q

R

A

A

A

V

S

I

V

T

V

A

A

L

L

E

N

A

G

P

P

V

Q

S

D

K

F

I

L

Q

K

Y

E

M

R

V

T

D

E

E

S

F

F

K

Q

E

R

R

R

R

R

K

D

L

L

I

V

L

V

G

N

L

G

L

L

N

N

D

A

I

I

E

D

G

G

F

L

E

D

C

C

N

R

E

V

P

P

E

E

G

G

A

A

F

F

Y

Y

V

T

F

F

P

S

N

G

I

C

S

A

H

G

Y

V

F

L

G

G

K

K

-

V

T

T

L

P

N

S

G

G

T

K

T

R

I

I

N

K

N

T

A

D

S

T

D

D

F

F

A

C

L

A

Y

Y

I

L

L

L

E

E

Q

D

A

A

N

H

V

V

A

A

T

V

V

V

T

P

G

G

E

S

A

A

F

F

G

G

N

L

P

S

N

P

C

F

I

F

R

R

I

I

S

S

Y

Y

A

A

A

T

S

S

Q

E

D

A

Q

E

I

L

K

K

E

E

A

A

L

L

A

E

R

R

I

I

K

A

K

A

A

A

binding pyridoxal-5'-phosphate: G99 (= G103), A100 (= A104), K101 (= K105), D204 (= D208), M206 (≠ I210), Y207 (= Y211), S238 (≠ A240), K239 (= K241), R247 (= R249)

8wkjA The crystal structure of aspartate aminotransferases lpg0070 from legionella pneumophila (see paper)
47% identity, 98% coverage: 8:398/398 of query aligns to 5:391/391 of 8wkjA

query
sites
8wkjA

L

L

S

A

D

K

R

R

I

V

N

Q

N

K

L

V

A

K

A

-

S

S

A

P

T

T

L

L

A

A

M

V

A

A

A

A

K

K

A

A

R

A

E

Q

L

M

R

K

A

A

E

Q

G

G

K

L

D

D

I

I

I

I

G

G

L

L

S

G

L

T

G

G

E

E

P

P

D

D

F

F

N

D

T

T

P

P

D

Q

F

H

I

I

K

K

D

L

A

A

A

A

I

I

Q

S

A

A

V

I

N

E

D

A

N

G

Y

D

N

T

S

K

Y

Y

T

T

P

A

V

V

D

D

G

G

Y

I

V

V

E

E

L

L

K

K

D

E

A

A

I

V

I

K

T

N

K

K

F

F

K

K

R

R

D

D

N

N

N

E

L

L

T

D

Y

Y

D

Q

R

L

S

N

Q

Q

I

I

V

L

V

V

S

S

T

V

G
|
G

A
x
G

K
|
K

Q

Q

S

S

L

C

Y

Y

N

N

V

L

A

C

Q

Q

V

A

C

Y

L

L

N

N

P

P

G

G

D

D

E

E

V

V

L

I

L

I

P

P

C

A

P

P

Y

Y

W
|
W

V

V

S

S

Y

Y

S

P

D

D

I

M

V

V

K

L

L

L

A

A

E

D

G

G

V

V

P

P

V

V

E

I

V

I

E

E

T

T

S

T

L

P

D

A

T

Q

D

R

F

Y

K

K

M

I

T

N

P

A

E

Q

Q

Q

L

L

E

E

A

Q

A

A

I

I

T

T

P

P

K

K

T

T

K

R

M

M

L

I

W

F

Y

L

S
x
N

S

S

P

P

C

S

N
|
N

P

P

S

S

G

G

S

I

I

A

Y

Y

S

T

E

Q

A

N

E

E

L

L

R

K

A

E

L

L

A

G

D

D

V

V

L

L

Q

K

K

K

H

H

P

P

Q

Q

I

I

V

L

V

I

V

A

S

T

D
|
D

E

D

I

M

Y
|
Y

E

E

H

H

I

I

N

I

Y

W

V

S

G

Q

G

P

H

F

A

T

S

N

M

I

A

L

Q

N

-

A

F

C

E

P

D

E

M

L

Y

Y

D

D

R

R

V

T

V

I

T

V

V

L

N

N

G

G

V

V

A
x
S

K
|
K

A

A

F

Y

A

A

M

M

T

T

G

G

W

W

R
|
R

I

I

G

G

Y

Y

I

A

G

A

A

G

P

P

A

A

Y

P

I

L

A

I

R

N

A

A

C

M

N

K

K

T

I

I

Q

Q

G

S

Q

Q

V

S

T

T

S

S

G

N

A

P

N

C

C

S

I

I

A

A

Q

Q

R

R

A

A

V

A

I

V

T

A

A

A

L

L

E

N

A

G

P

S

V

N

S

E

K

S

I

I

Q

E

Y

E

M

M

V

V

D

N

E

A

F

F

K

H

E

Q

R

R

R

H

K

D

L

Y

I

V

L

A

G

D

L

R

L

L

N

Q

D

S

I

I

E

D

G

G

F

I

E

E

C

V

N

I

E

P

P

A

E

D

G

G

A

T

F

F

Y

Y

V

I

F

F

P

P

N

S

I

V

S

Q

H

A

Y

I

F

I

G

E

K

K

T

R

L

G

N

Y

G

A

T

N

T

D

I

I

N

-

N

-

A

-

S

-

D

E

F

F

A

S

L

E

Y

K

I

L

L

L

E

N

Q

E

A

V

N

G

V

V

A

A

T

L

V

V

T

P

G

G

E

S

A

A

F

F

G

G

N

T

P

E

N

G

C

C

I

I

R

R

I

I

S

S

Y

F

A

A

A

T

S

G

Q

I

D

D

Q

T

I

L

K

K

E

D

A

A

L

L

A

N

R

R

I

L

K

Q

K

R

A

F

V

C

S

S

binding pyridoxal-5'-phosphate: G99 (= G103), G100 (≠ A104), K101 (= K105), W125 (= W129), N171 (≠ S175), N175 (= N179), D204 (= D208), Y207 (= Y211), S237 (≠ A240), K238 (= K241), R246 (= R249)

1j32A Aspartate aminotransferase from phormidium lapideum
43% identity, 98% coverage: 7:395/398 of query aligns to 2:382/388 of 1j32A

query
sites
1j32A

Q

K

L

L

S

A

D

A

R

R

I

V

N

E

N

S

L

V

A

S

A

P

S

S

A

M

T

T

L

L

A

I

M

I

A

D

A

A

K

K

A

A

R

K

E

A

L

M

R

K

A

A

E

E

G

G

K

I

D

D

I

V

I

C

G

S

L

F

S

S

L

A

G

G

E

E

P

P

D

D

F

F

N

N

T

T

P

P

D

K

F

H

I

I

K

V

D

E

A

A

A

A

I

K

Q

A

A

A

V

L

N

E

D

Q

N

G

Y

K

N

T

S

R

Y

Y

T

G

P

P

V

A

D

A

G

G

Y

E

V

P

E

R

L

L

K

R

D

E

A

A

I

I

I

A

T

Q

K

K

F

L

K

Q

R

R

D

D

N

N

N

G

L

L

T

C

Y

Y

D

G

R

A

S

D

Q

N

I

I

V

L

V

V

S

T

T

N

G
|
G

A
x
G

K
|
K

Q

Q

S

S

L

I

Y

F

N

N

V

L

A

M

Q

L

V

A

C

M

L

I

N

E

P

P

G

G

D

D

E

E

V

V

L

I

L

I

P

P

C

A

P

P

Y

F

W
|
W

V

V

S

S

Y

Y

S

P

D

E

I

M

V

V

K

K

L

L

A

A

E

E

G

G

V

T

P

P

V

V

E

I

V

L

E

P

T

T

S

T

L

V

D

E

T

T

D

Q

F

F

K

K

M

V

T

S

P

P

E

E

Q

Q

L

I

E

R

A

Q

A

A

I

I

T

T

P

P

K

K

T

T

K

K

M

L

L

L

W

V

Y

F

S

N

S

T

P

P

C

S

N
|
N

P

P

S

T

G

G

S

M

I

V

Y

Y

S

T

E

P

A

D

E

E

L

V

R

R

A

A

L

I

A

A

D

Q

V

V

L

A

Q

V

K

E

H

A

P

-

Q

G

I

L

V

W

V

V

V

L

S

S

D
|
D

E

E

I
|
I

Y
|
Y

E

E

H

K

I

I

N

L

Y

Y

V

D

G

D

G

A

-

Q

H

H

A

L

S

S

M

I

-

G

A

A

Q

A

F

S

E

P

D

E

M

A

Y

Y

D

E

R

R

V

S

V

V

T

V

V

C

N

S

G

G

V

F

A

A

K
|
K

A

T

F

Y

A

A

M

M

T

T

G

G

W

W

R
|
R

I

V

G

G

Y

F

I

L

G

A

A

G

P

P

A

V

Y

P

I

L

A

V

R

K

A

A

C

A

N

T

K

K

I

I

Q

Q

G

G

Q

H

V

S

T

T

S

S

G

N

A

V

N

C

C

T

I

F

A

A

Q

Q

R

Y

A

G

V

A

I

I

T

A

A

A

L

Y

E

E

A

N

P

S

V

Q

S

D

K

C

I

V

Q

Q

Y

E

M

M

V

L

D

A

E

A

F

F

K

A

E

E

R

R

R

R

K

R

L

Y

I

M

L

L

G

D

L

A

L

L

N

N

D

A

I

M

E

P

G

G

F

L

E

E

C

C

N

P

E

K

P

P

E

D

G

G

A

A

F

F

Y

Y

V

M

F

F

P

P

N

S

I

I

S

A

H

K

Y

-

F

-

G

-

K

-

T

-

L

-

N

-

G

-

T

-

T

T

I

G

N

R

N

S

A

S

S

L

D

D

F

F

A

C

L

S

Y

E

I

L

L

L

E

D

Q

Q

A

H

N

Q

V

V

A

A

T

T

V

V

T

P

G

G

E

A

A

A

F

F

G

G

N

A

P

D

N

D

C

C

I

I

R

R

I

L

S

S

Y

Y

A

A

A

T

S

D

Q

L

D

D

Q

T

I

I

K

K

E

R

A

G

L

M

A

E

R

R

I

L

K

E

K

K

active site: W124 (= W129), D202 (= D208), I204 (= I210), K237 (= K241)
binding pyridoxal-5'-phosphate: G98 (= G103), G99 (≠ A104), K100 (= K105), W124 (= W129), N174 (= N179), D202 (= D208), I204 (= I210), Y205 (= Y211), K237 (= K241), R245 (= R249)

Q56232 Aspartate/prephenate aminotransferase; AspAT / PAT; Transaminase A; EC 2.6.1.1; EC 2.6.1.78 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see 3 papers)
40% identity, 98% coverage: 8:397/398 of query aligns to 4:382/385 of Q56232

query
sites
Q56232

L

L

S

S

D

R

R

R

I

V

N

Q

N

A

L

M

A
x
K

A

P

S

S

A

A

T

T

L

V

A

A

M

V

A

N

A

A

K

K

A

A

R

L

E

E

L

L

R

R

A

R

E

Q

G

G

K

V

D

D

I

L

I

V

G

A

L

L

S

T

L

A

G

G

E

E

P

P

D

D

F

F

N

D

T

T

P

P

D

E

F

H

I

V

K

K

D

E

A

A

A

A

I

R

Q

R

A

A

V

L

N

A

D

Q

N

G

Y

K

N

T

S

K

Y

Y

T

A

P

P

V

P

D

A

G

G

Y

I

V

P

E

E

L

L

K

R

D

E

A

A

I

L

I

A

T

E

K

K

F

F

K

R

R

R

D

E

N

N

N

G

L

L

T

S

Y

V

D

T

R

P

S

E

Q

E

I

T

V

I

V

V

S

T

T

V

G

G

A

G

K

K

Q

Q

S

A

L

L

Y

F

N

N

V

L

A

F

Q

Q

V

A

C

I

L

L

N

D

P

P

G

G

D

D

E

E

V

V

L

I

L

V

P

L

C

S

P

P

Y

Y

W

W

V

V

S

S

Y

Y

S

P

D

E

I

M

V

V

K

R

L

F

A

A

E

G

G

G

V

V

P

V

V

V

E

E

V

V

E

E

T

T

S

L

L

P

D

E

T

E

D

G

F

F

K

V

M

P

T

D

P

P

E

E

Q

R

L

V

E

R

A

R

A

A

I

I

T

T

P

P

K

R

T

T

K

K

M

A

L

L

W

V

Y

V

S

N

S

S

P

P

C

N

N

N

P

P

S

T

G

G

S

A

I

V

Y

Y

S

P

E

K

A

E

E

V

L

L

R

E

A

A

L

L

A

A

D

R

V

L

L

A

Q

V

K

E

H

H

P

-

Q

D

I

F

V

Y

V

L

V

V

S

S

D

D

E

E

I

I

Y

Y

E

E

H

H

I

L

N

L

Y

Y

V

E

G

G

G

E

H

H

A

F

S

S

M

P

A

G

Q

R

F

V

E

A

D

P

M

-

Y

-

D

E

R

H

V

T

V

L

T

T

V

V

N

N

G

G

V

A

A

A

K
|
K

A

A

F

F

A

A

M

M

T

T

G

G

W

W

R

R

I

I

G

G

Y

Y

I

A

G

C

A

G

P

P

A

K

Y

E

I

V

A

I

R

K

A

A

C

M

N

A

K

S

I

V

Q

S

G

S

Q

Q

V

S

T

T

S

T

G

S

A

P

N

D

C

T

I

I

A

A

Q

Q

R

W

A

A

V

T

I

L

T

E

A

A

L

L

-

T

-

N

-

Q

E

E

A

A

P

S

V

R

S

A

K

F

I

V

Q

E

Y

M

M

A

V

R

D

E

E

A

F

Y

K

R

E

R

R

R

R

R

K

D

L

L

I

L

L

L

G

E

L

G

L

L

N

T

D

A

I

L

E

-

G

G

F

L

E

K

C

A

N

V

E

R

P

P

E

S

G

G

A

A

F

F

Y

Y

V

V

F

L

P

-

N

-

I

-

S

-

H

-

Y

-

F

-

G

-

K

-

T

-

L

M

N

D

G

T

T

S

T

P

I

I

N

A

N

P

A

D

S

E

D

V

F

R

A

A

L

A

Y

E

I

R

L

L

E

L

Q

E

A

A

N

G

V

V

A

A

T

V

V

V

T

P

G

G

E

T

A

D

F

F

G

A

N

A

P

F

N

G

C

H

I

V

R

R

I

L

S

S

Y

Y

A

A

A

T

S

S

Q

E

D

E

Q

N

I

L

K

R

E

K

A

A

L

L

A

E

R

R

I

F

K

A

K

R

A

V

V

L

K12 (≠ A16) Important for prephenate aminotransferase activity; mutation to G: 10-fold increase in Km for prephenate. Does not affect Km for oxaloacetate.
K234 (= K241) modified: N6-(pyridoxal phosphate)lysine

1bkgA Aspartate aminotransferase from thermus thermophilus with maleate (see paper)
40% identity, 98% coverage: 8:397/398 of query aligns to 4:382/382 of 1bkgA

query
sites
1bkgA

L

L

S

S

D

R

R

R

I

V

N

Q

N

A

L

M

A

K

A

P

S

S

A

A

T

T

L

V

A

A

M

V

A

N

A

A

K

K

A

A

R

L

E

E

L

L

R

R

A

R

E

Q

G

G

K

V

D

D

I

L

I

V

G

A

L

L

S

T

L

A

G

G

E

E

P

P

D

D

F

F

N

D

T

T

P

P

D

E

F

H

I

V

K

K

D

E

A

A

A

A

I

R

Q

R

A

A

V

L

N

A

D

Q

N

G

Y

K

N

T

S

K

Y

Y

T

A

P

P

V

P

D

A

G

G

Y

I

V

P

E

E

L

L

K

R

D

E

A

A

I

L

I

A

T

E

K

K

F

F

K

R

R

R

D

E

N

N

N

G

L

L

T

S

Y

V

D

T

R

P

S

E

Q

E

I

T

V

I

V

V

S

T

T

V

G
|
G

A
x
G

K
|
K

Q

Q

S

A

L

L

Y

F

N

N

V

L

A

F

Q

Q

V

A

C

I

L

L

N

D

P

P

G

G

D

D

E

E

V

V

L

I

L

V

P

L

C

S

P

P

Y

Y

W
|
W

V

V

S

S

Y

Y

S

P

D

E

I

M

V

V

K

R

L

F

A

A

E

G

G

G

V

V

P

V

V

V

E

E

V

V

E

E

T

T

S

L

L

P

D

E

T

E

D

G

F

F

K

V

M

P

T

D

P

P

E

E

Q

R

L

V

E

R

A

R

A

A

I

I

T

T

P

P

K

R

T

T

K

K

M

A

L

L

W

V

Y

V

S
x
N

S

S

P

P

C

N

N
|
N

P

P

S

T

G

G

S

A

I

V

Y

Y

S

P

E

K

A

E

E

V

L

L

R

E

A

A

L

L

A

A

D

R

V

L

L

A

Q

V

K

E

H

H

P

-

Q

D

I

F

V

Y

V

L

V

V

S

S

D
|
D

E

E

I
|
I

Y
|
Y

E

E

H

H

I

L

N

L

Y

Y

V

E

G

G

G

E

H

H

A

F

S

S

M

P

A

G

Q

R

F

V

E

A

D

P

M

-

Y

-

D

E

R

H

V

T

V

L

T

T

V

V

N

N

G

G

V

A

A

A

K
|
K

A

A

F

F

A

A

M

M

T

T

G

G

W

W

R
|
R

I

I

G

G

Y

Y

I

A

G

C

A

G

P

P

A

K

Y

E

I

V

A

I

R

K

A

A

C

M

N

A

K

S

I

V

Q

S

G

S

Q

Q

V

S

T

T

S

T

G

S

A

P

N

D

C

T

I

I

A

A

Q

Q

R

W

A

A

V

T

I

L

T

E

A

A

L

L

-

T

-

N

-

Q

E

E

A

A

P

S

V

R

S

A

K

F

I

V

Q

E

Y

M

M

A

V

R

D

E

E

A

F

Y

K

R

E

R

R

R

R

R

K

D

L

L

I

L

L

L

G

E

L

G

L

L

N

T

D

A

I

L

E

-

G

G

F

L

E

K

C

A

N

V

E

R

P

P

E

S

G

G

A

A

F

F

Y

Y

V

V

F

L

P

-

N

-

I

-

S

-

H

-

Y

-

F

-

G

-

K

-

T

-

L

M

N

D

G

T

T

S

T

P

I

I

N

A

N

P

A

D

S

E

D

V

F

R

A

A

L

A

Y

E

I

R

L

L

E

L

Q

E

A

A

N

G

V

V

A

A

T

V

V

V

T

P

G

G

E

T

A

D

F

F

G

A

N

A

P

F

N

G

C

H

I

V

R

R

I

L

S

S

Y

Y

A

A

A

T

S

S

Q

E

D

E

Q

N

I

L

K

R

E

K

A

A

L

L

A

E

R

R

I

F

K

A

K

R

A

V

V

L

active site: W125 (= W129), D203 (= D208), I205 (= I210), K234 (= K241)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G99 (= G103), G100 (≠ A104), K101 (= K105), W125 (= W129), N171 (≠ S175), N175 (= N179), D203 (= D208), I205 (= I210), Y206 (= Y211), K234 (= K241), R242 (= R249)

1bjwA Aspartate aminotransferase from thermus thermophilus (see paper)
40% identity, 98% coverage: 8:397/398 of query aligns to 4:382/382 of 1bjwA

query
sites
1bjwA

L

L

S

S

D

R

R

R

I

V

N

Q

N

A

L

M

A

K

A

P

S

S

A

A

T

T

L

V

A

A

M

V

A

N

A

A

K

K

A

A

R

L

E

E

L

L

R

R

A

R

E

Q

G

G

K

V

D

D

I

L

I

V

G

A

L

L

S

T

L

A

G
|
G

E

E

P

P

D

D

F

F

N

D

T

T

P

P

D

E

F

H

I

V

K

K

D

E

A

A

A

A

I

R

Q

R

A

A

V

L

N

A

D

Q

N

G

Y

K

N

T

S

K

Y

Y

T

A

P

P

V

P

D

A

G

G

Y

I

V

P

E

E

L

L

K

R

D

E

A

A

I

L

I

A

T

E

K

K

F

F

K

R

R

R

D

E

N

N

N

G

L

L

T

S

Y

V

D

T

R

P

S

E

Q

E

I

T

V

I

V

V

S

T

T

V

G

G

A

G

K

K

Q

Q

S

A

L

L

Y

F

N

N

V

L

A

F

Q

Q

V

A

C

I

L

L

N

D

P

P

G

G

D

D

E

E

V

V

L

I

L

V

P

L

C

S

P

P

Y

Y

W
|
W

V

V

S

S

Y

Y

S

P

D

E

I

M

V

V

K

R

L

F

A

A

E

G

G

G

V

V

P

V

V

V

E

E

V

V

E

E

T

T

S

L

L

P

D

E

T

E

D

G

F

F

K

V

M

P

T

D

P

P

E

E

Q

R

L

V

E

R

A

R

A

A

I

I

T

T

P

P

K

R

T

T

K

K

M

A

L

L

W

V

Y

V

S

N

S

S

P

P

C

N

N
|
N

P

P

S

T

G

G

S

A

I

V

Y

Y

S

P

E

K

A

E

E

V

L

L

R

E

A

A

L

L

A

A

D

R

V

L

L

A

Q

V

K

E

H

H

P

-

Q

D

I

F

V

Y

V

L

V

V

S

S

D
|
D

E

E

I
|
I

Y

Y

E

E

H

H

I

L

N

L

Y

Y

V

E

G

G

G

E

H

H

A

F

S

S

M

P

A

G

Q

R

F

V

E

A

D

P

M

-

Y

-

D

E

R

H

V

T

V

L

T

T

V

V

N

N

G

G

V

A

A

A

K
|
K

A

A

F

F

A

A

M

M

T

T

G

G

W

W

R

R

I

I

G

G

Y

Y

I

A

G

C

A

G

P

P

A

K

Y

E

I

V

A

I

R

K

A

A

C

M

N

A

K

S

I

V

Q

S

G

S

Q

Q

V

S

T

T

S

T

G

S

A

P

N

D

C

T

I

I

A

A

Q

Q

R

W

A

A

V

T

I

L