SitesBLAST
Comparing WP_009780078.1 NCBI__GCF_000152985.1:WP_009780078.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
7ucwB Structure of mouse decr1 in complex with 2'-5' oligoadenylate (see paper)
46% identity, 95% coverage: 7:284/293 of query aligns to 17:281/281 of 7ucwB
- binding [[(2R,3R,4R,5R)-5-(6-aminopurin-9-yl)-4-[[(2R,3R,4R,5R)-5-(6-aminopurin-9-yl)-4-[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl]oxy-3-hydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl]oxy-3-hydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl] phosphono hydrogen phosphate: G32 (= G22), T35 (≠ S25), S56 (= S46), R57 (= R47), N58 (= N48), D83 (= D73), V84 (= V74), A111 (= A101), A112 (= A102), I133 (= I123)
Q16698 2,4-dienoyl-CoA reductase [(3E)-enoyl-CoA-producing], mitochondrial; 2,4-dienoyl-CoA reductase [NADPH]; 4-enoyl-CoA reductase [NADPH]; Short chain dehydrogenase/reductase family 18C member 1; EC 1.3.1.124 from Homo sapiens (Human) (see paper)
47% identity, 99% coverage: 3:291/293 of query aligns to 47:333/335 of Q16698
- GGGTGL 66:71 (≠ GGGSGL 22:27) binding NADP(+)
- R91 (= R47) binding NADP(+)
- D117 (= D73) binding NADP(+)
- R119 (= R75) binding substrate
- N148 (= N104) mutation to A: Reduces enzyme activity by 97%.
- F149 (= F105) binding substrate
- S157 (= S113) binding substrate
- Y199 (= Y156) mutation to A: Reduces enzyme activity by 99%. Strongly reduced affinity for substrate and for NADP.
- S210 (= S167) mutation to A: Reduces enzyme activity by over 99%.
- K214 (= K171) binding NADP(+); mutation to A: Reduces enzyme activity by over 99%.
- PGPI 240:243 (≠ PGPF 197:200) binding NADP(+)
1w6uA Structure of human decr ternary complex (see paper)
47% identity, 97% coverage: 3:286/293 of query aligns to 13:288/288 of 1w6uA
- active site: G36 (= G26), T162 (≠ V153), A166 (= A157), V173 (= V164), P175 (= P166), K180 (= K171)
- binding hexanoyl-coenzyme a: N114 (= N104), F115 (= F105), S123 (= S113), N125 (= N115), T129 (≠ V119), Y165 (= Y156), P208 (= P199)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G32 (= G22), T35 (≠ S25), L37 (= L27), S56 (= S46), R57 (= R47), K58 (≠ N48), D83 (= D73), V84 (= V74), N110 (= N100), A111 (= A101), A112 (= A102), I161 (= I152), T162 (≠ V153), K180 (= K171), P208 (= P199)
Q9NUI1 Peroxisomal 2,4-dienoyl-CoA reductase [(3E)-enoyl-CoA-producing]; pDCR; 2,4-dienoyl-CoA reductase 2; Short chain dehydrogenase/reductase family 17C member 1; EC 1.3.1.124 from Homo sapiens (Human) (see paper)
40% identity, 86% coverage: 11:263/293 of query aligns to 24:273/292 of Q9NUI1
- GGGSGI 35:40 (≠ GGGSGL 22:27) binding NADP(+)
- RSLPR 60:64 (≠ RNLEK 47:51) binding NADP(+)
- D86 (= D73) binding NADP(+); mutation to A: Reduces enzyme activity by 98%.
- D137 (≠ V124) mutation to A: Reduces enzyme activity by 97%.
- K182 (= K171) binding NADP(+)
- D186 (≠ L175) mutation to A: Reduces enzyme activity by about 95%.
- 208:214 (vs. 197:203, 43% identical) binding NADP(+)
- D268 (= D258) mutation to A: Reduces enzyme activity by 97%.
4fc6B Studies on dcr shed new light on peroxisomal beta-oxidation: crystal structure of the ternary complex of pdcr (see paper)
40% identity, 86% coverage: 11:263/293 of query aligns to 22:271/276 of 4fc6B
- active site: G37 (= G26), A163 (≠ T154), Q173 (≠ V164), H175 (≠ P166), A176 (≠ S167), K180 (= K171)
- binding hexanoyl-coenzyme a: G114 (= G103), N115 (= N104), F116 (= F105), S124 (= S113), N126 (= N115), A127 (= A116), T130 (≠ V119), L165 (≠ Y156), R217 (= R208)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G33 (= G22), S36 (= S25), G37 (= G26), I38 (≠ L27), S57 (= S46), R58 (= R47), S59 (≠ N48), R62 (≠ K51), M83 (≠ C72), D84 (= D73), V85 (= V74), C111 (≠ N100), A112 (= A101), A113 (= A102), I134 (= I123), I161 (= I152), K180 (= K171), P206 (= P197), G207 (= G198), T212 (≠ K203), E213 (≠ G204), G214 (≠ A205)
4fc7A Studies on dcr shed new light on peroxisomal beta-oxidation: crystal structure of the ternary complex of pdcr (see paper)
40% identity, 86% coverage: 11:263/293 of query aligns to 21:270/274 of 4fc7A
- active site: G36 (= G26), A162 (≠ T154), Q172 (≠ V164), H174 (≠ P166), A175 (≠ S167), K179 (= K171)
- binding coenzyme a: R57 (= R47), R85 (= R75), G113 (= G103), N114 (= N104), F115 (= F105), S123 (= S113), N125 (= N115), A126 (= A116), T129 (≠ V119), R216 (= R208)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G32 (= G22), S35 (= S25), G36 (= G26), I37 (≠ L27), S56 (= S46), R57 (= R47), S58 (≠ N48), R61 (≠ K51), D83 (= D73), V84 (= V74), R85 (= R75), C110 (≠ N100), A111 (= A101), A112 (= A102), I160 (= I152), K179 (= K171), P205 (= P197), G206 (= G198), T211 (≠ K203), E212 (≠ G204), G213 (≠ A205)
Q9BY49 Peroxisomal trans-2-enoyl-CoA reductase; TERP; 2,4-dienoyl-CoA reductase-related protein; DCR-RP; HPDHase; Short chain dehydrogenase/reductase family 29C member 1; pVI-ARL; EC 1.3.1.38 from Homo sapiens (Human) (see paper)
34% identity, 90% coverage: 13:277/293 of query aligns to 16:295/303 of Q9BY49
Sites not aligning to the query:
- 303 mutation Missing: Abolishes localization to peroxisomes.
1yxmB Crystal structure of peroxisomal trans 2-enoyl coa reductase
33% identity, 90% coverage: 13:277/293 of query aligns to 9:280/283 of 1yxmB
- active site: G22 (= G26), V152 (≠ T154), G157 (= G160), H163 (≠ P166), S164 (= S167), R168 (≠ K171)
- binding adenine: S42 (= S46), R43 (= R47), C72 (= C72), N73 (≠ D73), I74 (≠ V74), T123 (≠ I123)
1vl8B Crystal structure of gluconate 5-dehydrogenase (tm0441) from thermotoga maritima at 2.07 a resolution
35% identity, 85% coverage: 13:260/293 of query aligns to 4:248/252 of 1vl8B
- active site: G17 (= G26), S143 (≠ T154), I154 (≠ V164), Y157 (≠ S167), K161 (= K171)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G22), R16 (≠ S25), G17 (= G26), L18 (= L27), S37 (= S46), R38 (= R47), C63 (= C72), D64 (= D73), V65 (= V74), A91 (≠ N100), A92 (= A101), G93 (≠ A102), I94 (≠ G103), V114 (≠ I123), I141 (= I152), S143 (≠ T154), Y157 (≠ S167), K161 (= K171), P187 (= P197), G188 (= G198), Y190 (≠ F200), T192 (= T202), M194 (≠ W206), T195 (≠ E207)
7pcsB Structure of the heterotetrameric sdr family member bbscd (see paper)
34% identity, 86% coverage: 13:263/293 of query aligns to 2:244/247 of 7pcsB
- binding nicotinamide-adenine-dinucleotide: G11 (= G22), M16 (≠ L27), D35 (≠ S46), I36 (≠ R47), I62 (≠ V74), N88 (= N100), G90 (≠ A102), I138 (= I152), S140 (≠ T155), Y152 (≠ S167), K156 (= K171), I185 (≠ F200)
Q9KJF1 (2S)-[(R)-hydroxy(phenyl)methyl]succinyl-CoA dehydrogenase subunit BbsD; (S,R)-2-(alpha-hydroxybenzyl)succinyl-CoA dehydrogenase subunit BbsD; EC 1.1.1.429 from Thauera aromatica (see 2 papers)
34% identity, 86% coverage: 13:263/293 of query aligns to 3:245/248 of Q9KJF1
- S15 (= S25) binding NAD(+)
- D36 (≠ S46) binding NAD(+)
- D62 (= D73) binding NAD(+)
- I63 (≠ V74) binding NAD(+)
- N89 (= N100) binding NAD(+)
- Y153 (≠ S167) binding NAD(+)
- K157 (= K171) binding NAD(+)
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
4nbuB Crystal structure of fabg from bacillus sp (see paper)
35% identity, 85% coverage: 13:260/293 of query aligns to 5:241/244 of 4nbuB
- active site: G18 (= G26), N111 (≠ V124), S139 (≠ N151), Q149 (≠ S161), Y152 (≠ S167), K156 (= K171)
- binding acetoacetyl-coenzyme a: D93 (≠ I106), K98 (≠ R111), S139 (≠ N151), N146 (≠ W158), V147 (≠ T159), Q149 (≠ S161), Y152 (≠ S167), F184 (≠ P199), M189 (≠ G204), K200 (= K217)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G22), N17 (≠ S25), G18 (= G26), I19 (≠ L27), D38 (≠ S46), F39 (≠ R47), V59 (≠ C72), D60 (= D73), V61 (= V74), N87 (= N100), A88 (= A101), G89 (≠ A102), I90 (≠ G103), T137 (≠ K147), S139 (≠ N151), Y152 (≠ S167), K156 (= K171), P182 (= P197), F184 (≠ P199), T185 (≠ F200), T187 (= T202), M189 (≠ G204)
3ay6B Crystal structure of bacillus megaterium glucose dehydrogenase 4 a258f mutant in complex with nadh and d-glucose (see paper)
32% identity, 85% coverage: 13:260/293 of query aligns to 11:255/267 of 3ay6B
- active site: G24 (= G26), S151 (≠ T154), Y164 (≠ S167), K168 (= K171)
- binding beta-D-glucopyranose: E102 (≠ N104), S151 (≠ T154), H153 (≠ Y156), W158 (≠ S161), Y164 (≠ S167), N202 (≠ A205), K205 (≠ R208)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G20 (= G22), T23 (≠ S25), G24 (= G26), L25 (= L27), Y45 (≠ R47), D71 (= D73), V72 (= V74), N98 (= N100), A99 (= A101), G100 (≠ A102), V101 (≠ G103), M149 (≠ I152), S151 (≠ T154), Y164 (≠ S167), K168 (= K171), P194 (= P197), G195 (= G198), M197 (≠ F200), T199 (= T202), P200 (≠ K203), I201 (≠ G204), N202 (≠ A205)
5u9pB Crystal structure of a gluconate 5-dehydrogenase from burkholderia cenocepacia j2315 in complex with NADP and tartrate
31% identity, 85% coverage: 13:260/293 of query aligns to 14:256/261 of 5u9pB
- active site: G27 (= G26), S152 (≠ T154), Y165 (≠ S167), K169 (= K171)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G23 (= G22), R26 (≠ S25), G27 (= G26), I28 (≠ L27), R48 (= R47), D73 (= D73), V74 (= V74), N100 (= N100), A101 (= A101), I150 (= I152), Y165 (≠ S167), K169 (= K171), P195 (= P197), F198 (= F200), T200 (= T202), L202 (≠ W206), N203 (≠ E207)
7x5jC Acp-dependent oxoacyl reductase
32% identity, 85% coverage: 12:260/293 of query aligns to 1:250/256 of 7x5jC
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G22), S13 (≠ G24), G15 (= G26), L16 (= L27), N36 (≠ S46), R37 (= R47), N38 (= N48), D62 (= D73), V63 (= V74), N89 (= N100), A90 (= A101), A91 (= A102), V114 (≠ I123), I141 (= I152), Y156 (≠ S167), K160 (= K171), L186 (≠ P197), G187 (= G198), V188 (≠ P199), R194 (≠ A205), S197 (≠ R208), S198 (≠ L209)
3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
32% identity, 85% coverage: 16:263/293 of query aligns to 5:246/246 of 3osuA