SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_009781898.1 NCBI__GCF_000152985.1:WP_009781898.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q96329 Acyl-coenzyme A oxidase 4, peroxisomal; AOX 4; G6p; Short-chain acyl-CoA oxidase; AtCX4; AtG6; SAOX; EC 1.3.3.6 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
47% identity, 86% coverage: 64:452/454 of query aligns to 41:428/436 of Q96329

query
sites
Q96329

L

F

P

P

I

C

D

T

G

S

D

D

F

Y

Y

Y

N

H

L

F

A

N

Q

D

K

L

L

L

T

T

P

P

E

E

R

Q

E

A

I

I

Q

R

M

K

K

K

M

V

R

R

N

E

F

C

M

M

E

E

D

K

E

E

V

V

K

A

P

P

I

I

A

M

N

T

D

E

F

Y

W

W

N

E

R

K

A

A

E

E

F

F

P

P

H

F

E

H

I

I

I

T

P

P

K

K

F

L

A

G

E

A

L

M

N

G

L

V

A

A

G

G

I

G

A

S

Y

I

K

K

G

G

Y

Y

G

G

C

C

P

P

G

G

Q

L

S

S

F

I

L

T

L

A

E

N

G

A

I

I

L

A

A

T

M

A

E

E

L

I

A

A

R

R

V

V

D

D

V

A

S

S

I

C

S

S

T

T

F

F

F

I

G

L

V

V

H

H

S

S

G

S

L

L

A

G

M

M

G

L

S

T

I

I

Y

A

L

L

C

C

G

G

S

S

E

E

E

A

Q

Q

K

K

Q

E

E

K

W

Y

L

L

P

P

K

S

M

L

Q

A

K

Q

F

L

E

N

K

T

I

V

G

A

A

C

F
x
W

G
x
A

L
|
L

T
|
T

E

E

P

P

E

D

V

N

G

G

S
|
S

G
x
D

A
|
A

A
x
S

G

G

G

-

L

L

G

G

T

T

S

T

C

A

K

T

K

K

V

V

G

E

D

G

E

G

W

W

I

K

L

I

N

N

G

G

E

Q

K

K

K

R

W

W

I

I

G
|
G

N

N

A

S

T

T

F

F

S

A

D

D

I

L

T

L

I

I

W

F

A

A

R

R

D

N

E

T

D

T

S

T

G

N

S

Q

V

I

K

N

G

G

F

F

I

I

V

V

R

K

K

K

E

D

N

A

P

P

G

G

F

L

H

K

A

A

E

T

K

K

M

I

K

P

D

N

K

K

M

I

A

G

L

L

R

R

T

M

V

V

Q

Q

N

N

A

G

L

D

I

I

T

L

L

L

T

Q

D

N

C

V

R

F

V

V

P

P

E

D

S

E

D

D

R

R

L

L

Q

P

N

G

A

V

N

N

S

S

F

F

K

Q

D

D

T

T

A

S

K

K

V

V

L

L

Q

A

M

V

T

S

R
|
R

A

V

S

M

V

V

A

A

W

W

Q

Q

A

P

V

I

G

G

C

I

A

S

R

M

G

G

A

I

Y

Y

E

D

A

M

A

C

L

H

K

R

Y

Y

T

L

K

K

K

E

R
|
R

E
x
K

Q
|
Q

F

F

G

G

R

A

P

P

I

L

A

A

S

A

F

F

Q
|
Q

L

L

I

N

Q

Q

N

Q

H

K

L

L

V

V

E

Q

M

M

I

L

S

G

N

N

L

V

T

Q

A

A

M

M

Q

F

T

L

L

M

C

G

F

W

R

R

L

L

S

C

E

K

M

L

Q

Y

D

E

G

T

G

G

Q

Q

L

M

T

T

D

P

E

G

H

Q

A

A

S

S

L

L

A

G

K

K

V

A

F

W

C

I

S

S

M

S

R

K

T

A

R

R

D

E

V

T

V

A

S

S

R

L

A

G

R

R

E
|
E

V

L

M

L

G

G

G
|
G

N

N

G

G

I

I

L

L

L

A

E

D

Y

F

D

L

V

V

A

A

R

K

F

A

V

F

A

C

D

D

A

L

E

E

A

P

I

I

Y

Y

S

T

Y

Y

E

E

G
|
G

T
|
T

K
x
Y

E
x
D

I

I

N

N

S

T

L

L

I

V

V

T

G

G

R

R

A

E

I

V

T

T

G

G

Y

I

S

A

A

S

F
|
F

WALT 172:175 (≠ FGLT 195:198) binding FAD
S181 (= S204) binding FAD
SDAS 181:184 (≠ SGAA 204:207) binding an acyl-CoA
G207 (= G231) binding FAD
R288 (= R312) binding an acyl-CoA
RKQ 313:315 (≠ REQ 337:339) binding FAD
Q324 (= Q348) binding FAD
E381 (= E405) binding FAD
G385 (= G409) binding FAD
G409 (= G433) binding an acyl-CoA
TYD 410:412 (≠ TKE 434:436) binding FAD
F428 (= F452) binding FAD

2ix5A Short chain specific acyl-coa oxidase from arabidopsis thaliana, acx4 in complex with acetoacetyl-coa (see paper)
47% identity, 86% coverage: 64:452/454 of query aligns to 25:412/415 of 2ix5A

query
sites
2ix5A

L

F

P

P

I

C

D

T

G

S

D

D

F

Y

Y

Y

N

H

L

F

A

N

Q

D

K

L

L

L

T

T

P

P

E

E

R

Q

E

A

I

I

Q

R

M

K

K

K

M

V

R

R

N

E

F

C

M

M

E

E

D

K

E

E

V

V

K

A

P

P

I

I

A

M

N

T

D

E

F

Y

W

W

N

E

R

K

A

A

E

E

F

F

P

P

H

F

E

H

I

I

I

T

P

P

K

K

F

L

A

G

E

A

L

M

N

G

L

V

A

A

G

G

I

G

A

S

Y

I

K

K

G

G

Y

Y

G

G

C

C

P

P

G

G

Q

L

S

S

F

I

L

T

L

A

E

N

G

A

I

I

L

A

A

T

M

A

E

E

L

I

A

A

R

R

V

V

D

D

V

A

S

S

I

C

S

S

T

T

F

F

F

I

G

L

V

V

H

H

S

S

G

S

L

L

A

G

M

M

G

L

S

T

I

I

Y

A

L

L

C

C

G

G

S

S

E

E

E

A

Q

Q

K

K

Q

E

E

K

W

Y

L

L

P

P

K

S

M

L

Q

A

K

Q

F

L

E

N

K

T

I

V

G

A

A

C

F

W

G

A

L
|
L

T
|
T

E

E

P

P

E

D

V

N

G
|
G

S
|
S

G

D

A
|
A

A
x
S

G

G

G

-

L

L

G

G

T

T

S

T

C

A

K

T

K

K

V

V

G

E

D

G

E

G

W

W

I

K

L

I

N

N

G

G

E

Q

K

K

K

R

W
|
W

I

I

G

G

N

N

A

S

T

T

F

F

S

A

D

D

I

L

T

L

I

I

W

F

A

A

R

R

D

N

E

T

D

T

S

T

G

N

S

Q

V

I

K

N

G

G

F

F

I

I

V

V

R

K

K

K

E

D

N

A

P

P

G

G

F

L

H

K

A

A

E

T

K

K

M

I

K

P

D

N

K

K

M

I

A

G

L

L

R

R

T

M

V

V

Q

Q

N
|
N

A

G

L

D

I

I

T

L

L

L

T

Q

D

N

C

V

R

F

V

V

P

P

E

D

S

E

D

D

R

R

L

L

Q

P

N

G

A

V

N

N

S

S

F
|
F

K

Q

D

D

T

T

A

S

K

K

V

V

L
|
L

Q

A

M

V

T
x
S

R
|
R

A

V

S

M

V

V

A

A

W

W

Q

Q

A

P

V

I

G

G

C

I

A

S

R

M

G

G

A

I

Y

Y

E

D

A

M

A

C

L

H

K

R

Y

Y

T

L

K

K

K

E

R
|
R

E

K

Q

Q

F
|
F

G

G

R

A

P

P

I
x
L

A

A

S

A

F
|
F

Q

Q

L
|
L

I
x
N

Q

Q

N

Q

H

K

L

L

V

V

E

Q

M

M

I

L

S

G

N

N

L

V

T

Q

A

A

M

M

Q

F

T

L

L

M

C

G

F

W

R

R

L

L

S

C

E

K

M

L

Q

Y

D

E

G

T

G

G

Q

Q

L

M

T

T

D

P

E

G

H

Q

A

A

S

S

L

L

A

G

K

K

V

A

F

W

C

I

S

S

M

S

R

K

T

A

R

R

D

E

V

T

V

A

S

S

R

L

A

G

R

R

E
|
E

V
x
L

M

L

G
|
G

N

N

G

G

I

I

L

L

L

A

E

D

Y

F

D

L

V

V

A

A

R

K

F

A

V

F

A

C

D

D

A

L

E

E

A

P

I

I

Y

Y

S

T

Y
|
Y

E
|
E

G
|
G

T
|
T

K

Y

E
x
D

I
|
I

N

N

S

T

L

L

I

V

V

T

G

G

R
|
R

A

E

I

V

T

T

G

G

Y

I

S

A

A

S

F

F

active site: L158 (= L197), T159 (= T198), S271 (≠ T311), E392 (= E432), R404 (= R444)
binding acetoacetyl-coenzyme a: S165 (= S204), A167 (= A206), S168 (≠ A207), F261 (= F301), L268 (= L308), R272 (= R312), E392 (= E432), G393 (= G433), R404 (= R444)
binding flavin-adenine dinucleotide: L158 (= L197), T159 (= T198), G164 (= G203), S165 (= S204), W189 (= W229), N239 (= N279), R297 (= R337), F300 (= F340), L304 (≠ I344), F307 (= F347), L309 (= L349), N310 (≠ I350), E365 (= E405), L366 (≠ V406), G368 (= G408), G369 (= G409), Y391 (= Y431), T394 (= T434), D396 (≠ E436), I397 (= I437)

2ix6A Short chain specific acyl-coa oxidase from arabidopsis thaliana, acx4 (see paper)
47% identity, 86% coverage: 64:452/454 of query aligns to 25:412/416 of 2ix6A

query
sites
2ix6A

L

F

P

P

I

C

D

T

G

S

D

D

F

Y

Y

Y

N

H

L

F

A

N

Q

D

K

L

L

L

T

T

P

P

E

E

R

Q

E

A

I

I

Q

R

M

K

K

K

M

V

R

R

N

E

F

C

M

M

E

E

D

K

E

E

V

V

K

A

P

P

I

I

A

M

N

T

D

E

F

Y

W

W

N

E

R

K

A

A

E

E

F

F

P

P

H

F

E

H

I

I

I

T

P

P

K

K

F

L

A

G

E

A

L

M

N

G

L

V

A

A

G

G

I

G

A

S

Y

I

K

K

G

G

Y

Y

G

G

C

C

P

P

G

G

Q

L

S

S

F

I

L

T

L

A

E

N

G

A

I

I

L

A

A

T

M

A

E

E

L

I

A

A

R

R

V

V

D

D

V

A

S

S

I

C

S

S

T

T

F

F

F

I

G

L

V

V

H

H

S

S

G

S

L

L

A

G

M

M

G

L

S

T

I

I

Y

A

L

L

C

C

G

G

S

S

E

E

E

A

Q

Q

K

K

Q

E

E

K

W

Y

L

L

P

P

K

S

M

L

Q

A

K

Q

F

L

E

N

K

T

I

V

G

A

A

C

F

W

G

A

L
|
L

T
|
T

E

E

P

P

E

D

V

N

G
|
G

S
|
S

G

D

A

A

A

S

G

G

G

-

L

L

G

G

T

T

S

T

C

A

K

T

K

K

V

V

G

E

D

G

E

G

W

W

I

K

L

I

N

N

G

G

E

Q

K

K

K

R

W
|
W

I

I

G

G

N

N

A

S

T

T

F

F

S

A

D

D

I

L

T

L

I

I

W

F

A

A

R

R

D

N

E

T

D

T

S

T

G

N

S

Q

V

I

K

N

G

G

F

F

I

I

V

V

R

K

K

K

E

D

N

A

P

P

G

G

F

L

H

K

A

A

E

T

K

K

M

I

K

P

D

N

K

K

M

I

A

G

L

L

R

R

T

M

V

V

Q

Q

N
|
N

A

G

L

D

I

I

T

L

L

L

T

Q

D

N

C

V

R

F

V

V

P

P

E

D

S

E

D

D

R

R

L

L

Q

P

N

G

A

V

N

N

S

S

F

F

K

Q

D

D

T

T

A

S

K

K

V

V

L

L

Q

A

M

V

T
x
S

R

R

A

V

S

M

V

V

A

A

W

W

Q

Q

A

P

V

I

G

G

C

I

A

S

R

M

G

G

A

I

Y

Y

E

D

A

M

A

C

L

H

K

R

Y

Y

T

L

K

K

K

E

R
|
R

E

K

Q

Q

F
|
F

G

G

R

A

P

P

I
x
L

A

A

S

A

F
|
F

Q

Q

L

L

I
x
N

Q

Q

N

Q

H

K

L

L

V

V

E

Q

M

M

I

L

S

G

N

N

L

V

T

Q

A

A

M

M

Q

F

T

L

L

M

C

G

F

W

R

R

L

L

S

C

E

K

M

L

Q

Y

D

E

G

T

G

G

Q

Q

L

M

T

T

D

P

E

G

H

Q

A

A

S

S

L

L

A

G

K

K

V

A

F

W

C

I

S

S

M

S

R

K

T

A

R

R

D

E

V

T

V

A

S

S

R

L

A

G

R

R

E
|
E

V
x
L

M

L

G

G

G
|
G

N

N

G

G

I
|
I

L

L

L

A

E

D

Y

F

D

L

V

V

A

A

R

K

F

A

V

F

A

C

D

D

A

L

E

E

A

P

I

I

Y

Y

S

T

Y
|
Y

E
|
E

G

G

T
|
T

K

Y

E
x
D

I

I

N

N

S

T

L

L

I

V

V

T

G

G

R
|
R

A

E

I

V

T

T

G

G

Y

I

S

A

A

S

F

F

active site: L158 (= L197), T159 (= T198), S271 (≠ T311), E392 (= E432), R404 (= R444)
binding flavin-adenine dinucleotide: T159 (= T198), G164 (= G203), S165 (= S204), W189 (= W229), N239 (= N279), R297 (= R337), F300 (= F340), L304 (≠ I344), F307 (= F347), N310 (≠ I350), E365 (= E405), L366 (≠ V406), G369 (= G409), I372 (= I412), Y391 (= Y431), T394 (= T434), D396 (≠ E436)

2ebaA Crystal structure of the putative glutaryl-coa dehydrogenase from thermus thermophilus
42% identity, 84% coverage: 70:452/454 of query aligns to 3:380/380 of 2ebaA

query
sites
2ebaA

D

D

F

F

Y

Y

N

A

L

L

A

E

Q

D

K

L

L

L

T

T

P

P

E

E

R

K

E

E

I

V

Q

Q

M

K

K

A

M

A

R

R

N

R

F

F

M

L

E

E

D

K

E

E

V

A

K

L

P

P

I

H

A

I

N

R

D

D

F

W

W

W

N

E

R

E

A

G

E

V

F

F

P

P

H

T

E

H

I

L

I

I

P

P

K

R

F

F

A

A

E

E

L

L

N

G

L

F

A

L

G

G

I

P

A

T

Y

L

K

P

G

P

-

E

Y

Y

G

G

C

G

P

A

G

G

Q

V

S

S

F

S

L

A

E

Y

G

G

I

L

L

I

A

C

M

Y

E

E

L

L

A

E

R

R

V

V

D

D

V

S

S

G

I

L

S

R

T

S

F

F

F

V

G

S

V

V

H

Q

S

S

G

S

L

L

A

V

M

M

G

Y

S

P

I

I

Y

Y

L

A

C

Y

G

G

S

S

E

E

Q

Q

K

K

Q

R

E

E

W

F

L

L

P

P

K

K

M

L

Q

A

K

R

F

G

E

E

K

M

I

V

G

G

A

C

F

F

G

G

L
|
L

T
|
T

E

E

P

P

E

D

V
x
G

G
|
G

S
|
S

G

D

A

P

A

Y

G

G

G

N

L

M

G

K

T

T

S

R

C

A

K

R

V

-

G

-

D

D

E

T

W

W

I

V

L

L

N

N

G

G

E

T

K

K

K

M

W
|
W

I

I

G
x
T

N

N

A

G

T

N

F

L

S

A

D

H

I

L

T

A

I

V

I

I

W

W

A

A

R

K

D

D

E

E

D

-

S

-

G

-

S

-

V

V

K

L

G

G

F

F

I

L

V

V

R

P

K

T

E

D

N

T

P

P

G

G

F

F

H

Q

A

A

E

R

K

E

M

V

K

K

D

R

K

K

M

M

A

S

L

L

R

R

T

A

V

S

Q

V

N

T

A

S

L

E

I

L

T

V

L

L

T

E

D

E

C

V

R

R

V

V

P

P

E

E

S

S

D

L

R

R

L

L

Q

P

N

K

A

A

N

L

S

G

F

L

K

K

D

A

T

P

A

L

K

S

V

C

L

L

Q

T

M

Q

T
x
A

R

R

A

F

S

G

V

I

A

A

W

W

Q

G

A

A

V

M

G

G

C

A

A

L

R

E

G

A

A

V

Y

Y

E

E

A

E

A

A

L

V

K

A

Y

F

T

A

K

K

K

S

R
|
R

E

S

Q

T

F

F

G

G

R

E

P

P

I
x
L

A

A

S

K

F
x
K

Q

Q

L

L

I

V

Q

Q

N

A

H

K

L

L

V

A

E

E

M

M

I

L

S

A

N

W

L

H

T

T

A

E

M

G

Q

L

T

L

L

L

C

A

F

W

R

R

L

L

S

A

E

R

M

L

Q

K

D

D

G

E

G

G

Q

K

L

L

T

T

D

P

E

A

H

Q

A

V

S

S

L

L

A

A

K

K

V

R

F

Q

C

N

S

V

M

W

R

K

T

A

R

L

D

Q

V

A

S

R

R

M

A

A

R

R

E
x
D

V
x
I

M

L

G

G

G
|
G

N

S

G

G

I

I

L

T

L

L

E

E

Y

Y

D

H

V

A

A

I

R

R

F

H

V

M

A

L

D

N

A

L

E

E

A
x
T

I

V

Y

Y

S
x
T

Y
|
Y

E
|
E

G

G

T
|
T

K

H

E

D

I

V

N

H

S

T

L

L

I

V

V

L

G

G

R
|
R

A

E

I

I

T

T

G

G

Y

L

S

N

A

A

F

F

active site: L131 (= L197), T132 (= T198), A239 (≠ T311), E360 (= E432), R372 (= R444)
binding flavin-adenine dinucleotide: L131 (= L197), T132 (= T198), G136 (≠ V202), G137 (= G203), S138 (= S204), W161 (= W229), T163 (≠ G231), R265 (= R337), L272 (≠ I344), K275 (≠ F347), D333 (≠ E405), I334 (≠ V406), G337 (= G409), T355 (≠ A427), T358 (≠ S430), Y359 (= Y431), T362 (= T434)

3sf6A Crystal structure of glutaryl-coa dehydrogenase from mycobacterium smegmatis (see paper)
38% identity, 84% coverage: 70:452/454 of query aligns to 6:386/387 of 3sf6A

query
sites
3sf6A

D

D

F

L

Y

I

N

G

L

I

A

N

Q

A

K

V

L

L

T

S

P

A

E

E

R

R

E

E

I

I

Q

R

M

D

K

T

M

V

R

R

N

S

F

V

M

V

E

Q

D

R

E

R

V

I

K

K

P

P

I

H

A

I

N

A

D

S

F

W

W

Y

N

E

R

D

A

G

E

E

F

L

P

P

-

A

H

R

E

E

I

L

I

A

P

V

K

E

F

L

A

G

E

E

L

L

N

G

L

L

A

L

G

G

I

M

A

H

Y

L

K

K

G

G

Y

Y

G

G

C

C

P

A

G

G

Q

M

S

S

F

A

L

V

L

A

E

Y

G

G

I

L

L

A

A

C

M

L

E

E

L

L

A

E

R

A

V

G

D

D

V

S

S

G

I

I

S

R

T

S

F

L

F

V

G

S

V

V

H

Q

S

G

G

S

L

L

A

A

M

M

G

Y

S

A

I

I

Y

H

L

A

C

F

G

G

S

S

E

D

E

E

Q

Q

K

K

Q

D

E

Q

W

W

L

L

P

P

K

D

M

M

Q

A

K

S

F

G

E

H

K

R

I

I

G

G

A

C

F
|
F

G

G

L
|
L

T
|
T

E

E

P

P

E

D

V

H

G
|
G

S
|
S

G

D

A

P

A

A

G

G

G

-

L

M

G

R

T

T

S

R

C

A

K

T

K

R

V

S

G

G

D

D

E

D

W

W

I

I

L

L

N

T

G

G

E

T

K

K

K

M

W
|
W

I
|
I

G
x
T

N

N

A

G

T

S

F

V

S

A

D

D

I

V

T

A

I

V

W

W

A

A

R

R

D

T

E

D

D

E

S

G

G

-

S

-

V

I

K

R

G

G

F

F

I

V

V

V

R

P

K

T

E

D

N

T

P

P

G

G

F

F

H

T

A

A

E

N

K

T

M

I

K

K

D

S

K

K

M

M

A

S

L

L

R

R

T

A

V

S

Q

V

N

T

A

S

L

E

I

L

T

V

L

L

T

D

D

G

C

V

R

R

V

L

P

P

E

D

S

S

D

A

R

R

L

L

Q

P

N

G

A

A

N

T

S

S

F

L

K

G

D

A

T

P

A

L

K

R

V

C

L

L

Q

N

M

E

T
x
A

R

R

A

F

S

G

V

I

A

V

W

F

Q

G

A

A

V

L

G

G

C

A

A

A

R

R

G

D

A

C

Y

L

E

E

A

T

A

A

L

L

K

A

Y

Y

T

A

K

C

K

S

R

R

E

E

Q

Q

F

F

G

D

R

R

P

P

I

I

A

G

S

G

F

F

Q

Q

L

L

I

T

Q

Q

N

Q

H

K

L

L

V

A

E

D

M

M

I

T

S

L

N

E

L

Y

T

G

A

K

M

G

Q

F

T

L

L

L

C

A

F

L

R

H

L

L

S

G

E

R

M

Q

Q

K

D

D

G

A

G

G

Q

E

L

L

T

A

D

P

E

E

H

Q

A

V

S

S

L

L

A

G

K

K

V

L

F

N

C

N

S

V

M

R

R

E

T

A

R

I

D

E

V

I

V

A

S

R

R

T

A

A

R

R

E

T

V

V

M

L

G

G

G

A

N

S

G

G

I

I

L

T

L

G

E

E

Y

Y

D

P

V

V

A

M

R

R

F

H

V

A

A

N

D

N

A

L

E

E

A
x
S

I

V

Y

L

S
x
T

Y
|
Y

E
|
E

G

G

T
|
T

K

S

E
|
E

I
x
M

N

H

S

T

L

L

I

I

V

I

G

G

R
x
Q

A

A

I

L

T

T

G

G

Y

V

S

G

A

A

F

F

active site: L134 (= L197), T135 (= T198), A245 (≠ T311), E366 (= E432), Q378 (≠ R444)
binding dihydroflavine-adenine dinucleotide: F132 (= F195), L134 (= L197), T135 (= T198), G140 (= G203), S141 (= S204), W165 (= W229), I166 (= I230), T167 (≠ G231), S361 (≠ A427), T364 (≠ S430), Y365 (= Y431), T368 (= T434), E370 (= E436), M371 (≠ I437)

3swoA Crystal structure of a glutaryl-coa dehydrogenase from mycobacterium smegmatis in complex with fadh2 (see paper)
35% identity, 84% coverage: 70:452/454 of query aligns to 8:387/388 of 3swoA

query
sites
3swoA

D

E

F

L

Y

F

N

D

L

T

A

D

Q

R

K

L

L

L

T

D

P

Q

E

D

E

E

R

R

E

D

I

I

Q

A

M

A

K

T

M

V

R

R

N

Q

F

F

M

V

E

D

D

T

E

R

V

L

K

K

P

P

I

N

A

V

N

E

D

G

F

W

W

F

N

E

R

S

A

A

E

T

F

L

P

P

H

S

E

E

I

L

I

A

P

K

K

E

F

F

A

G

E

N

L

L

N

G

L

V

A

L

G

G

I

M

A

H

Y

L

K

Q

G

G

Y

Y

G

G

C

C

P

A

G

G

Q

T

S

N

F

A

L

V

L

S

E

Y

G

G

I

L

L

A

A

C

M

M

E

E

L

L

A

E

R

A

V

G

D

D

V

S

S

G

I

F

S

R

T

S

F

F

F

V

G

S

V

V

H

Q

S

G

G

S

L

L

A

S

M

M

G

F

S

S

I

I

Y

Y

L

R

C

Y

G

G

S

S

E

E

Q

Q

K

K

Q

N

E

E

W

W

L

L

P

P

K

R

M

L

Q

A

K

A

F

G

E

D

K

A

I

I

G

G

A

C

F
|
F

G

G

L
|
L

T
|
T

E

E

P

P

E

D

V

F

G
|
G

S
|
S

G

N

A

P

A

A

G

G

G

-

L

M

G

R

T

T

S

R

C

A

K

R

V

D

G

G

D

S

E

D

W

W

I

I

L

L

N

N

G

G

E

T

K

K

K

M

W
|
W

I
|
I

G
x
T

N

N

A

G

T

N

F

L

S

A

D

D

I

V

T

A

I

T

I

V

W

W

A

A

R

Q

D

T

E

D

D

D

S

G

G

-

S

-

V

I

K

R

G

G

F

F

I

L

V

V

R

P

K

T

E

D

N

T

P

P

G

G

F

F

H

T

A

A

E

N

K

E

M

I

K

H

D

R

K

K

M

L

A

S

L

L

R

R

T

A

V

S

Q

V

N

T

A

S

L

E

I

L

T

V

L

L

T

D

D

N

C

V

R

R

V

L

P

P

E

A

S

S

D

A

R

Q

L

L

Q

P

N

L

A

A

N

E

S

G

F

L

K

S

D

A

T

P

A

L

K

S

V

C

L

L

Q

N

M

E

T
x
A

R

R

A

F

S

G

V

I

A

V

W

F

Q

G

A

A

V

L

G

G

C

A

A

A

R

R

G

D

A

S

Y

L

E

E

A

T

L

I

K

A

Y

Y

T

T

K

Q

K

S

R
|
R

E

E

Q
x
V

F
|
F

G

D

R

K

P

P

I
x
L

A

S

S

N

F
x
Y

Q

Q

L

L

I

T

Q

Q

N

E

H

K

L

L

V

A

E

N

M

M

I

T

S

V

N

E

L

L

T

G

A

K

M

G

Q

M

T

L

L

L

C

A

F

I

R

H

L

L

S

G

E

R

M

I

Q

K

D

D

G

A

G

E

Q

G

L

V

T

R

D

P

E

E

H

Q

A

I

S

S

L

L

A

G

K

K

V

L

F

-

C

-

S

-

M

N

R

N

T

V

R

R

D

E

V

A

V

I

S

A

R

I

A

A

R

R

E

E

-

C

-

R

-
x
T

V
x
L

M

L

G

G

G
|
G

N

S

G

G

I
|
I

L

T

L

L

E

E

Y

Y

D

S

V

P

A

L

R

R

F

H

V

A

A

N

D

N

A

L

E

E

A

S

I

V

Y

L

S
x
T

Y
|
Y

E
|
E

G

G

T
|
T

K

S

E
|
E

I
x
M

N

H

S

L

L

L

I

S

V

I

G

G

R
x
K

A

A

I

L

T

T

G

G

Y

K

S

A

A

A

F

F

active site: L135 (= L197), T136 (= T198), A246 (≠ T311), E367 (= E432), K379 (≠ R444)
binding dihydroflavine-adenine dinucleotide: F133 (= F195), L135 (= L197), T136 (= T198), G141 (= G203), S142 (= S204), W166 (= W229), I167 (= I230), T168 (≠ G231), R272 (= R337), V274 (≠ Q339), F275 (= F340), L279 (≠ I344), Y282 (≠ F347), T340 (vs. gap), L341 (≠ V406), G344 (= G409), I347 (= I412), T365 (≠ S430), Y366 (= Y431), T369 (= T434), E371 (= E436), M372 (≠ I437)

2r0nA The effect of a glu370asp mutation in glutaryl-coa dehydrogenase on proton transfer to the dienolate intermediate (see paper)
37% identity, 84% coverage: 74:454/454 of query aligns to 10:390/390 of 2r0nA

query
sites
2r0nA

L

L

A

E

Q

E

K

Q

L

L

T

T

P

T

E

D

E

E

R

I

E

L

I

I

Q

R

M

D

K

T

M

F

R

R

N

T

F

Y

M

C

E

Q

D

E

E

R

V

L

K

M

P

P

I

R

A

I

N

L

D

-

F

L

W

A

N

N

R

R

A

N

E

E

-

V

F

F

P

H

H

R

E

E

I

I

P

S

K

E

F

M

A

G

E

E

L

L

N

G

L

V

A

L

G

G

I

P

A

T

Y

I

K

K

G

G

Y

Y

G

G

C

C

P

A

G

G

Q

V

S

S

F

S

L

V

L

A

E

Y

G

G

I

L

L

L

A

A

M

R

E

E

L

L

A

E

R

R

V

V

D

D

V

S

S

G

I

Y

S
x
R

T
x
S

F

A

F

M

G

S

V
|
V

H

Q

S

S

G

S

L

L

A

V

M

M

G

H

S

P

I

I

Y

Y

L

A

C

Y

G

G

S

S

E

E

Q

Q

K

R

Q

Q

E

K

W

Y

L

L

P

P

K

Q

M

L

Q

A

K

K

F

G

E

E

K

L

I

L

G

G

A

C

F
|
F

G

G

L
|
L

T
|
T

E

E

P

P

E

N

V

S

G
|
G

S
|
S

G

D

A
x
P

A

S

G

S

G

M

L

E

G

T

T

R

S

A

C

H

K

Y

K

N

V

S

G

S

D

N

E

K

-

S

W

Y

I

T

L

L

N

N

G

G

E

T

K

K

K

T

W
|
W

I
|
I

G
x
T

N

N

A

S

T

P

F

M

S

A

D

D

I

L

T

F

I

V

W

W

A

A

R

R

D

C

E

E

D

D

S

-

G

G

S

C

V

I

K

R

G

G

F

F

I

L

V

L

R

E

K

K

E

G

N

M

P

R

G

G

F

L

H

S

A

A

E

P

K

R

M

I

K

Q

D

G

K

K

M

F

A

S

L

L

R

R

T

A

V

S

Q

A

N

T

A

G

L

M

I

I

T

I

L

M

T

D

D

G

C

V

R

E

V

V

P

P

E

E

S

E

D

N

R

V

L

L

Q

P

N

G

A

A

N

S

S

S

F

L

K
x
G

D

G

T

P

A
x
F

K

G

V

C

L
|
L

Q
x
N

M

N

T
x
A

R

R

A

Y

S

G

V

I

A

A

W

W

Q

G

A

V

V

L

G

G

C

A

A

S

R

E

G

F

A

C

Y

L

E

H

A

T

A

A

L

R

K

Q

Y

Y

T

A

K

L

K

D

R

R

E

M

Q

Q

F

F

G

G

R

V

P

P

I

L

A

A

S

R

F

N

Q

Q

L

L

I

I

Q

Q

N

K

H

K

L

L

V

A

E

D

M

M

I

L

S

T

N

E

L

I

T

T

A

L

M

G

Q

L

T

H

L

A

C

C

F

L

R

Q

L

L

S

G

E

R

M

L

Q

K

D

D

G

Q

G

D

Q

K

L

A

T

A

D
x
P

E

E

H

M

A

V

S

S

L

L

A

L

K

K

V

R

F

N

C

N

S

C

M

G

R

K

T

A

R

L

D

D

V

I

V

A

S

R

R

Q

A

A

R

R

E

D

V

M

M

L

G

G

N

N

G

G

I

I

L

S

L

D

E

E

Y

Y

D

H

V

V

A

I

R

R

F

H

V

A

A

M

D

N

A

L

E

E

A

A

I

V

Y

N

S

T

Y
|
Y

E
|
E

G

G

T
|
T

K

H

E
x
D

I

I

N

H

S

A

L

L

I
|
I

V

L

G

G

R
|
R

A

A

I

I

T

T

G

G

Y

I

S

Q

A

A

F

F

V

T

S

A

active site: L133 (= L197), T134 (= T198), A247 (≠ T311), E368 (= E432), R380 (= R444)
binding flavin-adenine dinucleotide: F131 (= F195), L133 (= L197), T134 (= T198), G139 (= G203), S140 (= S204), W166 (= W229), I167 (= I230), T168 (≠ G231), Y367 (= Y431), T370 (= T434), D372 (≠ E436)
binding 3-thiaglutaryl-CoA: R92 (≠ S156), S93 (≠ T157), V97 (= V161), P142 (≠ A206), G238 (≠ K302), F241 (≠ A305), L244 (= L308), N245 (≠ Q309), P318 (≠ D382), Y367 (= Y431), E368 (= E432), I377 (= I441)

1sirA The crystal structure and mechanism of human glutaryl-coa dehydrogenase (see paper)
37% identity, 84% coverage: 74:454/454 of query aligns to 10:390/390 of 1sirA

query
sites
1sirA

L

L

A

E

Q

E

K

Q

L

L

T

T

P

T

E

D

E

E

R

I

E

L

I

I

Q

R

M

D

K

T

M

F

R

R

N

T

F

Y

M

C

E

Q

D

E

E

R

V

L

K

M

P

P

I

R

A

I

N

L

D

-

F

L

W

A

N

N

R

R

A

N

E

E

-

V

F

F

P

H

H

R

E

E

I

I

P

S

K

E

F

M

A

G

E

E

L

L

N

G

L

V

A

L

G

G

I

P

A

T

Y

I

K

K

G

G

Y

Y

G

G

C

C

P

A

G

G

Q

V

S

S

F

S

L

V

L

A

E

Y

G

G

I

L

L

L

A

A

M

R

E

E

L

L

A

E

R

R

V

V

D

D

V

S

S

G

I

Y

S

R

T
x
S

F

A

F

M

G

S

V

V

H

Q

S

S

G

S

L

L

A

V

M

M

G

H

S

P

I

I

Y

Y

L

A

C

Y

G

G

S

S

E

E

Q

Q

K

R

Q

Q

E

K

W

Y

L

L

P

P

K

Q

M

L

Q

A

K

K

F

G

E

E

K

L

I

L

G

G

A

C

F
|
F

G

G

L
|
L

T
|
T

E

E

P

P

E

N

V

S

G
|
G

S
|
S

G

D

A

P

A

S

G

S

G

M

L

E

G

T

T

R

S

A

C

H

K

Y

K

N

V

S

G

S

D

N

E

K

-

S

W

Y

I

T

L

L

N

N

G

G

E

T

K

K

K

T

W
|
W

I
|
I

G
x
T

N

N

A

S

T

P

F

M

S

A

D

D

I

L

T

F

I

V

W

W

A

A

R

R

D

C

E

E

D

D

S

-

G

G

S

C

V

I

K

R

G

G

F

F

I

L

V

L

R

E

K

K

E

G

N

M

P

R

G

G

F

L

H

S

A

A

E

P

K

R

M

I

K

Q

D

G

K

K

M

F

A

S

L

L

R

R

T

A

V

S

Q

A

N

T

A

G

L

M

I

I

T

I

L

M

T

D

D

G

C

V

R

E

V

V

P

P

E

E

S

E

D

N

R

V

L

L

Q

P

N

G

A

A

N

S

S

S

F

L

K

G

D

G

T

P

A
x
F

K
x
G

V

C

L
|
L

Q
x
N

M

N

T
x
A

R
|
R

A

Y

S

G

V

I

A

A

W

W

Q

G

A

V

V

L

G

G

C

A

A

S

R

E

G

F

A

C

Y

L

E

H

A

T

A

A

L

R

K

Q

Y

Y

T

A

K

L

K

D

R

R

E

M

Q

Q

F

F

G

G

R

V

P

P

I

L

A

A

S

R

F

N

Q

Q

L

L

I

I

Q

Q

N

K

H

K

L

L

V

A

E

D

M

M

I

L

S

T

N

E

L

I

T

T

A

L

M

G

Q

L

T

H

L

A

C

C

F

L

R

Q

L

L

S

G

E

R

M

L

Q

K

D

D

G

Q

G

D

Q

K

L

A

T

A

D
x
P

E

E

H

M

A

V

S

S

L

L

A

L

K

K

V

R

F

N

C

N

S

C

M

G

R

K

T

A

R

L

D

D

V

I

V

A

S

R

R

Q

A

A

R

R

E

D

V

M

M

L

G

G

N

N

G

G

I

I

L

S

L

D

E

E

Y

Y

D

H

V

V

A

I

R

R

F

H

V

A

A

M

D

N

A

L

E

E

A

A

I

V

Y

N

S

T

Y
|
Y

E
|
E

G

G

T
|
T

K

H

E

D

I

I

N

H

S

A

L

L

I

I

V

L

G

G

R
|
R

A

A

I

I

T

T

G

G

Y

I

S

Q

A

A

F

F

V

T

S

A

active site: L133 (= L197), T134 (= T198), A247 (≠ T311), E368 (= E432), R380 (= R444)
binding flavin-adenine dinucleotide: F131 (= F195), L133 (= L197), T134 (= T198), G139 (= G203), S140 (= S204), W166 (= W229), I167 (= I230), T168 (≠ G231), Y367 (= Y431), T370 (= T434)
binding s-4-nitrobutyryl-coa: S93 (≠ T157), S140 (= S204), F241 (≠ A305), G242 (≠ K306), L244 (= L308), N245 (≠ Q309), R248 (= R312), P318 (≠ D382), Y367 (= Y431), E368 (= E432), R380 (= R444)

2r0mA The effect of a glu370asp mutation in glutaryl-coa dehydrogenase on proton transfer to the dienolate intermediate (see paper)
37% identity, 84% coverage: 74:454/454 of query aligns to 10:390/390 of 2r0mA

query
sites
2r0mA

L

L

A

E

Q

E

K

Q

L

L

T

T

P

T

E

D

E

E

R

I

E

L

I

I

Q

R

M

D

K

T

M

F

R

R

N

T

F

Y

M

C

E

Q

D

E

E

R

V

L

K

M

P

P

I

R

A

I

N

L

D

-

F

L

W

A

N

N

R

R

A

N

E

E

-

V

F

F

P

H

H

R

E

E

I

I

P

S

K

E

F

M

A

G

E

E

L

L

N

G

L

V

A

L

G

G

I

P

A

T

Y

I

K

K

G

G

Y

Y

G

G

C

C

P

A

G

G

Q

V

S

S

F

S

L

V

L

A

E

Y

G

G

I

L

L

L

A

A

M

R

E

E

L

L

A

E

R

R

V

V

D

D

V

S

S

G

I

Y

S

R

T

S

F

A

F

M

G

S

V

V

H

Q

S

S

G

S

L
|
L

A

V

M

M

G

H

S

P

I

I

Y

Y

L

A

C

Y

G

G

S

S

E

E

Q

Q

K

R

Q

Q

E

K

W

Y

L

L

P

P

K

Q

M

L

Q

A

K

K

F

G

E

E

K

L

I

L

G

G

A

C

F
|
F

G

G

L
|
L

T
|
T

E

E

P

P

E

N

V

S

G
|
G

S
|
S

G

D

A

P

A

S

G

S

G

M

L

E

G

T

T

R

S

A

C

H

K

Y

K

N

V

S

G

S

D

N

E

K

-

S

W

Y

I

T

L

L

N

N

G

G

E

T

K

K

K

T

W
|
W

I
|
I

G
x
T

N

N

A

S

T

P

F

M

S

A

D

D

I

L

T

F

I

V

W

W

A

A

R

R

D

C

E

E

D

D

S

-

G

G

S

C

V

I

K

R

G

G

F

F

I

L

V

L

R

E

K

K

E

G

N

M

P

R

G

G

F

L

H

S

A

A

E

P

K

R

M

I

K

Q

D

G

K

K

M

F

A

S

L
|
L

R

R

T

A

V

S

Q

A

N

T

A

G

L

M

I

I

T

I

L

M

T

D

D

G

C

V

R

E

V

V

P

P

E

E

S

E

D

N

R

V

L

L

Q

P

N

G

A

A

N

S

S

S

F

L

K

G

D

G

T

P

A

F

K

G

V

C

L

L

Q

N

M

N

T
x
A

R

R

A

Y

S

G

V

I

A

A

W

W

Q

G

A

V

V

L

G

G

C

A

A

S

R

E

G

F

A

C

Y

L

E

H

A

T

A

A

L

R

K

Q

Y

Y

T

A

K

L

K

D

R

R

E

M

Q

Q

F

F

G

G

R

V

P

P

I

L

A

A

S

R

F

N

Q

Q

L

L

I

I

Q

Q

N

K

H

K

L

L

V

A

E

D

M

M

I

L

S

T

N

E

L

I

T

T

A

L

M

G

Q

L

T

H

L

A

C

C

F

L

R

Q

L

L

S

G

E

R

M

L

Q

K

D

D

G

Q

G

D

Q

K

L

A

T

A

D

P

E

E

H

M

A

V

S

S

L

L

A

L

K

K

V

R

F

N

C

N

S

C

M

G

R

K

T

A

R

L

D

D

V

I

V

A

S

R

R

Q

A

A

R

R

E

D

V

M

M

L

G

G

N

N

G

G

I

I

L

S

L

D

E

E

Y

Y

D

H

V

V

A

I

R

R

F

H

V

A

A

M

D

N

A

L

E

E

A

A

I

V

Y

N

S

T

Y
|
Y

E
x
D

G

G

T
|
T

K

H

E

D

I

I

N

H

S

A

L

L

I

I

V

L

G

G

R
|
R

A

A

I

I

T

T

G

G

Y

I

S

Q

A

A

F

F

V

T

S

A

active site: L133 (= L197), T134 (= T198), A247 (≠ T311), D368 (≠ E432), R380 (= R444)
binding 4-nitrobutanoic acid: L101 (= L165), Y367 (= Y431), D368 (≠ E432)
binding flavin-adenine dinucleotide: F131 (= F195), L133 (= L197), T134 (= T198), G139 (= G203), S140 (= S204), W166 (= W229), I167 (= I230), T168 (≠ G231), L210 (= L274), Y367 (= Y431), T370 (= T434)

5ol2F The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
36% identity, 78% coverage: 91:442/454 of query aligns to 17:372/378 of 5ol2F

query
sites
5ol2F

N

S

F

F

M

A

E

E

D

N

E

E

V

V

K

K

P

P

I

L

A

A

N

T

D
x
E

F

L

W

D

N

E

R
x
E

A

E

E
x
R

F

F

P

P

H

Y

E

E

I

T

I

V

P

E

K

K

F

M

A

A

E

K

L

A

N

G

L

M

A

M

G

G

I

I

A

P

Y

Y

-

P

K

K

G

E

Y

Y

G

G

C

G

P

E

G

G

Q

G

S

D

F

T

L

V

L

G

E

Y

G

I

I

M

L

A

A

V

M

E

E

E

L

L

A

S

R

R

V

V

D

C

V

G

S

T

I

T

S

G

T

V

F

I

F

L

G

S

V

A

H

H

S

T

G

S

L

L

A

G

M

S

G

W

S

P

I

I

Y

Y

L

Q

C

Y

G

G

S

N

E

E

Q

Q

K

K

Q

Q

E

K

W

F

L

L

P

R

K

P

M

L

Q

A

K

S

F

G

E

E

K

K

I

L

G

G

A

A

F
|
F

G

G

L
|
L

T
|
T

E

E

P
|
P

E

N

V

A

G

G

S
x
T

G

D

A

A

A

S

G

G

G

Q

L

Q

G

T

T

T

S

A

C

V

K

L

K

D

V

-

G

G

D

D

E

E

W

Y

I

I

L

L

N

N

G

G

E

S

K

K

K

I

W
x
F

I
|
I

G
x
T

N

N

A

A

T

I

F

A

S

G

D

D

I

I

T

Y

I

V

W

M

A

A

R

M

D

T

E

D

D

K

S

S

G

K

S

G

V

N

K

K

G

G

-

I

-

S

-

A

F

F

I

I

V

V

R

E

K

K

E

G

N

T

P

P

G

G

F

F

H

S

A

F

E

G

K

V

M

K

K
x
E

D

K

K

K

M

M

A

G

L

I

R

R

T

G

V

S

Q

A

N

T

A

S

L

E

I

L

T

I

L

F

T

E

D

D

C

C

R

R

V

I

P

P

E

K

S

E

D

N

R

L

L

L

-

G

Q

K

N

E

A

G

N

Q

S

G

F

F

K

K

D

I

T

A

A

M

K

S

V

T

L
|
L

Q

D

M

G

T
x
G

R
|
R

A

I

S

G

V

I

A

A

W

A

Q

Q

A

A

V

L

G

G

C

L

A

A

R

Q

G

G

A

A

Y

L

E

D

A

E

A

T

L

V

K

K

Y

Y

T

V

K

K

K

E

R
|
R

E

V

Q

Q

F
|
F

G

G

R

R

P

P

I
x
L

A

S

S

K

F
|
F

Q

Q

L

N

I

T

Q

Q

N

F

H

Q

L

L

V

A

E

D

M

M

I

E

S

V

N

K

L

V

T

Q

A

A

M

A

Q

R

T

H

L

L

C

V

F

Y

R

Q

L

A

S

A

E

I

M

N

Q

K

D

D

G

L

G

G

Q

K

L

P

T

Y

D

G

E

V

H

E

A

A

S

A

L

M

A

A

K

K

V

L

F

F

C

A

S

A

M

E

R

T

T

A

R

M

D

E

V

V

V

T

S

T

R

K

A

A

R

V

E
x
Q

V
x
L

M

H

G
|
G

N

Y

G

G

I

Y

L

T

L

R

E

D

Y

Y

D

P

V

V

A

E

R

R

F

M

V

M

A

R

D

D

A

A

E

K

A

I

I

T

Y

E

S

I

Y
|
Y

E
|
E

G
|
G

T
|
T

K

S

E
|
E

I

V

N

Q

S

R

L

M

I

V

V

I

active site: L124 (= L197), T125 (= T198), G241 (≠ T311)
binding calcium ion: E29 (≠ D103), E33 (≠ R107), R35 (≠ E109)
binding coenzyme a persulfide: L238 (= L308), R242 (= R312), E362 (= E432), G363 (= G433)
binding flavin-adenine dinucleotide: F122 (= F195), L124 (= L197), T125 (= T198), P127 (= P200), T131 (≠ S204), F155 (≠ W229), I156 (= I230), T157 (≠ G231), E198 (≠ K269), R267 (= R337), F270 (= F340), L274 (≠ I344), F277 (= F347), Q335 (≠ E405), L336 (≠ V406), G338 (= G408), G339 (= G409), Y361 (= Y431), T364 (= T434), E366 (= E436)

Sites not aligning to the query:

active site: 374

3gncA Crystal structure of glutaryl-coa dehydrogenase from burkholderia pseudomallei with fragment 6421 (see paper)
34% identity, 83% coverage: 74:451/454 of query aligns to 12:380/380 of 3gncA

query
sites
3gncA

L

L

A

D

Q

Q

K

Q

L

L

T

A

P

D

E

D

E

E

R

R

E

M

I

V

Q

R

M

D

K

A

M

A

R

H

N

A

F

Y

M

A

E

Q

D

G

E

K

V

L

K

A

P

P

I

R

A

V

N

T

D

E

F

A

W

F

N

R

R

H

A

E

E

T

F

T

P

D

H

A

E

A

I

I

I

F

P

R

K

E

F

M

A

G

E

E

L

I

N

G

L

L

A

L

G

G

I

P

A

T

Y

I

-

P

K

E

G

Q

Y

Y

G

G

C

G

P

P

G

G

Q

L

S

D

F

Y

L

V

L

S

E

Y

G

G

I

L

L

I

A

A

M

R

E

E

L

V

A

E

R

R

V

V

D

D

V

S

S

G

I

Y

S
x
R

T
x
S

F

M

G

S

V

V

H

Q

S

S

G

S

L

L

A

V

M

M

G

V

S

P

I

I

Y

F

L

E

C

F

G

G

S

S

E

D

E

A

Q

Q

K

K

Q

E

E

K

W

Y

L

L

P

P

K

K

M

L

Q

A

K

T

F

G

E

E

K

W

I

I

G

G

A

C

F

F

G

G

L
|
L

T
|
T

E

E

P

P

E

M

V

V

G

-

S

-

G

-

A

-

G

-

L

-

G

-

T

T

S

R

C

A

K

R

K

K

V

V

G

P

D

G

E

G

W

Y

I

S

L

L

N

S

G

G

E

S

K

K

K

M

W

W

I

I

G

T

N

N

A

S

T

P

F

I

S

A

D

D

I

V

T

F

I

V

W

W

A

A

R

K

-

L

D

D

E

E

D

D

S

R

G

D

S

E

V

I

K

R

G

G

F

F

I

I

V

L

R

E

K

K

E

G

N

C

P

K

G

G

F

L

H

S

A

A

E

P

K

A

M

I

K

H

D

G

K

K

M

V

A

G

L

L

R

R

T

A

V

S

Q

I

N

T

A

G

L

E

I

I

T

V

L

L

T

D

D

E

C

A

R

F

V

V

P

P

E

E

S

E

D

N

R

I

L

L

Q

P

N

H

A

V

N

K

S

G

F

L

K

R

D

G

T

P

A

F

K

T

V

C

L

L

Q

N

M

S

T
x
A

R

R

A

Y

S

G

V
x
I

A

A

W

W

Q

G

A

A

V

L

G

G

C

A

A

A

R

E

G

S

A

C

Y

W

E

H

A

I

A

A

L

R

K

Q

Y

Y

T

V

K

L

K

D

R

R

E

K

Q

Q

F

F

G

G

R

R

P

P

I

L

A

A

S

A

F

N

Q

Q

L

L

I

I

Q

Q

N

K

H

K

L

L

V

A

E

D

M

M

I

Q

S

T

N

E

L

I

T

T

A

L

M

G

Q

L

T

Q

L

G

C

V

F

L

R

R

L

L

S

G

E

R

M

M

Q

K

D

D

G

E

G

G

Q

T

L

A

T

A

D

V

E

E

H

I

A

T

S

S

L

I

A

M

K

K

V

R

F

N

C

S

S

C

M

G

R

K

T

A

R

L

D

D

V

I

V

A

S

R

R

L

A

A

R

R

E

D

V

M

M

L

G

G

N

N

G

G

I

D

L

-

E

E

Y

F

D

G

V

V

A

A

R

R

F

H

V

L

A

V

D

N

A

L

E

E

A

V

I
x
V

Y

N

S

T

Y
|
Y

E
|
E

G

G

T

T

K

H

E

D

I

I

N

H

S

A

L

L

I

I

V

L

G

G

R
|
R

A

A

I

Q

T

T

G

G

Y

I

S

Q

A

A

active site: L136 (= L197), T137 (= T198), A242 (≠ T311), E361 (= E432), R373 (= R444)
binding 1-(1-methylethyl)-1H-benzimidazole-2-sulfonic acid: R95 (≠ S156), S96 (≠ T157), I246 (≠ V315), V357 (≠ I428), Y360 (= Y431), E361 (= E432)

3eonC 2.55a crystal structure of native glutaryl-coa dehydrogenase from burkholderia pseudomallei in complex with a small molecule (see paper)
33% identity, 83% coverage: 74:451/454 of query aligns to 11:382/382 of 3eonC

query
sites
3eonC

L

L

A

D

Q

Q

K

Q

L

L

T

A

P

D

E

D

E

E

R

R

E

M

I

V

Q

R

M

D

K

A

M

A

R

H

N

A

F

Y

M

A

E

Q

D

G

E

K

V

L

K

A