SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_010173582.1 NCBI__GCF_000171615.1:WP_010173582.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1sazA Membership in the askha superfamily: enzymological properties and crystal structure of butyrate kinase 2 from thermotoga maritima (see paper)
57% identity, 98% coverage: 5:362/367 of query aligns to 2:360/375 of 1sazA

query
sites
1sazA

F

F

R

R

I

I

L

L

V

T

I

I

N

N

P

P

G

G

S

S

T

T

S

S

T

T

K

K

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S

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I

F

F

D

E

N

D

D

E

D

R

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M

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V

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E

M

K

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H

H

D

S

S

P

D

D

E

E

I

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N

G

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Y

F

P

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S

K

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I

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D

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Q

Y

L

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F

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R

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K

T

I

I

A

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R

H

Q

A

F

L

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D

E

E

E

E

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G

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S

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S

A

A

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F

C

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R

G

G

L

L

R

D

P

P

I

I

E

P

G

G

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Y

Y

E

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N

D

E

G

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M

M

L

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K

D

T

L

L

R

K

K

S

G

G

Y

K

S

N

G

G

Q

E

H

H

A

A

S

S

N

N

L

L

G

G

G

A

I

I

A

A

N

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E

R

I

F

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G

S

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A

F

Y

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D

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D

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R

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K

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I

F

F

H
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H

A

A

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L

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N

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Q

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K

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A

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V

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M

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Y

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G

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A

A

A

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H

K

R

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K

G

G

K

R

V

V

I

I

D

D

V

V

N

N

G

A

L

L

H

D

G

G

D

D

G

G

P

P

F

F

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T

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E

R
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R

A

S

G

G

T

T

V

L

P

P

A

L

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D

Q

L

L

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V

D

D

L

L

C

C

F

F

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S

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G

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K

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Y

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R

Y

E

E

I

M

M

K

K

K

K

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G

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N

G

G

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L

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V

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A

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Y

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L

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G

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T

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x
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D

A
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A

I
x
R

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K

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R

R

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K

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G

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Y

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M

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Y

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K

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G

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D

A

F

I

I

I

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T

G

G

G
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G

L
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L

A

A

Y
x
H

G

E

K

K

D

E

F

F

-

L

V

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W

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W

F

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A

D

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P

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G

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N

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R

active site: R214 (= R217)
binding phosphomethylphosphonic acid adenylate ester: H154 (= H157), G184 (= G187), G185 (= G188), G186 (= G189), S254 (≠ N257), D255 (= D258), A256 (= A259), R257 (≠ I260), G304 (= G307), L305 (= L308), H307 (≠ Y310)

4ijnA Crystal structure of an acetate kinase from mycobacterium smegmatis bound to amp and sulfate (see paper)
34% identity, 42% coverage: 156:308/367 of query aligns to 160:312/376 of 4ijnA

query
sites
4ijnA

F

F

H
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H

A

G

L

T

N

S

Q

H

K

E

A

Y

V

V

A

S

R

Q

V

A

A

A

K

I

E

F

L

L

N

D

K

R

S

P

Y

L

E

E

E

S

L

L

N

N

L

Q

I

I

V

V

T

L

H

H

M

L

G
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G

G
x
N

G

G

I

A

T

S

V

A

G

S

A

A

H

V

K

A

G

G

K

K

V

A

I

V

D

D

V

T

N

S

N

M

G

G

L

L

H

T

G

P

-

M

D

E

G

G

P

L

F

V

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M

P

G

E

T

R
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R

A

S

G

G

T

D

V

I

P

D

A

P

G

G

D

V

L

I

V

M

D

Y

L

L

C

W

F

R

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T

G

A

D

G

F

M

Y

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E

D

I

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M

E

K

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K

M

L

L

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N

G

R

Q

R

G

S

G

G

L

V

V

L

G

G

Y

L

L

G

G

G

T

A

N

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D
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D

A
x
F

I
x
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Q

K

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E

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E

R

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I

E

E

K

S

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G

D

D

E

E

K

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A

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K

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A

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Y

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D

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M

Y

A

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R

K

K

E

Y

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G

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A

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S

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A

A

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-

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D

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I

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T

T

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A

G
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G

L
x
V

active site: H161 (= H157), R222 (= R217)
binding adenosine monophosphate: G191 (= G187), N192 (≠ G188), D263 (= D258), F264 (≠ A259), R265 (≠ I260), G311 (= G307), V312 (≠ L308)

Sites not aligning to the query:

active site: 8, 72, 365
binding adenosine monophosphate: 315, 316

P38502 Acetate kinase; Acetokinase; EC 2.7.2.1 from Methanosarcina thermophila (see 5 papers)
33% identity, 43% coverage: 152:308/367 of query aligns to 175:332/408 of P38502

query
sites
P38502

R

R

K

K

S

Y

I

G

F
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F

H

H

A

G

L

T

N

S

Q

H

K

K

A

Y

V

V

A

A

R

E

R

R

V

A

A

A

K

L

E

M

L

L

N

G

K

K

S

P

Y

A

E

E

L

T

N

K

L

I

I

I

V

T

T

C

H

H

M

L

G

G

G
x
N

G

G

I

S

T

S

V

I

G

T

A

A

H

V

K

E

G

G

K

K

V

S

I

V

D

E

V

T

N

S

N

M

G

G

L

-

H

-

G

-

D

-

G

-

P

-

F

F

S

T

P
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P

-

L

-

E

-

G

-

L

-

A

-

M

-

G

E

T

R
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R

A

C

G

G

T

S

V

I

P

D

A

P

G

A

D

I

L

V

V

P

D

F

L

L

C

M

F

E

S

K

G

E

D

G

F

L

Y

T

R

T

E

R

E

E

I

I

M

D

K

T

K

L

L

M

V

N

G

K

Q

K

G

S

G

G

L

V

V

L

G

G

Y

V

L

S

G

G

-

L

T

S

N

N

D

D

A

F

I

R

Q

D

V

L

E

D

K

E

R

A

I

A

E

S

K

K

G

G

D

N

E

R

K

K

A

A

K

E

L

L

V

A

Y

L

D

E

A

I

M

F

A

A

Y

Y

Q

K

V

V

A

K

K

K

E

F

I

I

G

G

S

E

A

Y

S

S

A

A

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V

L

L

Q

N

G

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Q

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V

A

D

D

A

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L

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T

T

G

A

G

G

L

I

F179 (= F156) mutation to A: Decreases affinity for acetate.
N211 (≠ G188) mutation to A: Slightly reduced enzyme activity.
P232 (= P215) mutation to A: Decreases affinity for acetate.
R241 (= R217) mutation R->K,L: Decreases catalytic activity. Strongly reduced affinity for ATP.

Sites not aligning to the query:

7 N→A: Almost abolishes catalytic activity. Requires increased magnesium levels for activity. Strongly decreases affinity for acetate.; N→D: Almost abolishes catalytic activity. Strongly decreases affinity for acetate.
10 mutation S->A,T: Strongly decreases catalytic activity. Strongly decreases affinity for acetate.
12 S→A: Decreases catalytic activity. Strongly decreases affinity for acetate. Requires increased magnesium levels for enzyme activity.; S→T: Decreases catalytic activity. Strongly decreases affinity for acetate.
14 K→A: Strongly decreases enzyme activity.; K→R: Reduces enzyme activity.
91 mutation R->A,L: Decreases catalytic activity. Decreases affinity for acetate.
93 V→A: Decreases affinity for acetate.
122 L→A: Decreases affinity for acetate.
148 active site, Proton donor/acceptor; mutation D->A,E,N: Abolishes catalytic activity. Decreases affinity for acetate, but not for ATP.
384 E→A: Almost abolishes catalytic activity. Strongly decreases affinity for acetate. Requires strongly increased magnesium levels for enzyme activity.

1tuuA Acetate kinase crystallized with atpgs (see paper)
33% identity, 43% coverage: 152:308/367 of query aligns to 175:332/399 of 1tuuA

query
sites
1tuuA

R

R

K

K

S

Y

I

G

F

F

H
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H

A

G

L

T

N

S

Q

H

K

K

A

Y

V

V

A

A

R

E

R

R

V

A

A

A

K

L

E

M

L

L

N

G

K

K

S

P

Y

A

E

E

L

T

N

K

L

I

I

I

V

T

T

C

H

H

M

L

G
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G

G

N

G
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G

I

S

T

S

V

I

G

T

A

A

H

V

K

E

G

G

K

K

V

S

I

V

D

E

V

T

N

S

N

M

G

G

L

-

H

-

G

-

D

-

G

-

P

-

F

F

S

T

P

P

-

L

-

E

-

G

-

L

-

A

-

M

-

G

E

T

R
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R

A

C

G

G

T

S

V

I

P

D

A

P

G

A

D

I

L

V

V

P

D

F

L

L

C

M

F

E

S

K

G

E

D

G

F

L

Y

T

R

T

E

R

E

E

I

I

M

D

K

T

K

L

L

M

V

N

G

K

Q

K

G

S

G

G

L

V

V

L

G

G

Y

V

L

S

G

G

-

L

T

S

N

N

D
|
D

A
x
F

I
x
R

Q

D

V

L

E

D

K

E

R

A

I

A

E

S

K

K

G

G

D

N

E

R

K

K

A

A

K

E

L

L

V

A

Y

L

D

E

A

I

M

F

A

A

Y

Y

Q

K

V

V

A

K

K

K

E

F

I

I

G

G

S

E

A

Y

S

S

A

A

V

V

L

L

Q

N

G

G

Q

-

V

A

D

D

A

A

I

V

L

F

T

T

G

A

G
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G

L
x
I

active site: H180 (= H157), R241 (= R217)
binding adenosine-5'-diphosphate: G210 (= G187), D283 (= D258), F284 (≠ A259), R285 (≠ I260), G331 (= G307), I332 (≠ L308)
binding sulfate ion: H180 (= H157), G212 (= G189)

Sites not aligning to the query:

active site: 7, 91, 384
binding adenosine-5'-diphosphate: 14, 335
binding sulfate ion: 91

1tuuB Acetate kinase crystallized with atpgs (see paper)
33% identity, 43% coverage: 152:308/367 of query aligns to 175:332/398 of 1tuuB

query
sites
1tuuB

R

R

K

K

S

Y

I

G

F

F

H
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H

A

G

L

T

N

S

Q

H

K

K

A

Y

V

V

A

A

R

E

R

R

V

A

A

A

K

L

E

M

L

L

N

G

K

K

S

P

Y

A

E

E

L

T

N

K

L

I

I

I

V

T

T

C

H

H

M

L

G

G

G

N

G
|
G

I

S

T

S

V

I

G

T

A

A

H

V

K

E

G

G

K

K

V

S

I

V

D

E

V

T

N

S

N

M

G

G

L

-

H

-

G

-

D

-

G

-

P

-

F

F

S

T

P

P

-

L

-

E

-

G

-

L

-

A

-

M

-

G

E

T

R
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R

A

C

G

G

T

S

V

I

P

D

A

P

G

A

D

I

L

V

V

P

D

F

L

L

C

M

F

E

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K

G

E

D

G

F

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Y

T

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T

E

R

E

E

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D

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T

K

L

L

M

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N

G

K

Q

K

G

S

G

G

L

V

V

L

G

G

Y

V

L

S

G

G

-

L

T

S

N

N

D
|
D

A

F

I
x
R

Q

D

V

L

E

D

K

E

R

A

I

A

E

S

K

K

G

G

D

N

E

R

K

K

A

A

K

E

L

L

V

A

Y

L

D

E

A

I

M

F

A

A

Y

Y

Q

K

V

V

A

K

K

K

E

F

I

I

G

G

S

E

A

Y

S

S

A

A

V

V

L

L

Q

N

G

G

Q

-

V

A

D

D

A

A

I

V

L

F

T

T

G

A

G
|
G

L
x
I

active site: H180 (= H157), R241 (= R217)
binding adenosine monophosphate: D283 (= D258), R285 (≠ I260), G331 (= G307), I332 (≠ L308)
binding trihydrogen thiodiphosphate: H180 (= H157), G212 (= G189), R241 (= R217)

Sites not aligning to the query:

active site: 7, 91, 384
binding adenosine monophosphate: 335, 336

4iz9A Crystal structure of an acetate kinase from mycobacterium avium bound to an unknown acid-apcpp conjugate and manganese (see paper)
26% identity, 94% coverage: 6:350/367 of query aligns to 5:381/381 of 4iz9A

query
sites
4iz9A

R

R

I

V

L

L

V

V

I

I

N
|
N

P

S

G

G

S

S

T

S

S

S

T

L

K
|
K

I

F

G

Q

V

L

F

V

D

D

N

P

D

E

D

S

V

G

V

V

F

A

E

A

K

S

T

T

L

-

R

-

H

G

D

I

S

V

D

E

E

R

I

I

N

G

Q

E

Y

S

P

P

S

V

I

P

I

D

D

H

Q

D

Y

A

E

A

F

L

R

R

K

R

T

A

T

F

I

D

L

M

H

L

A

A

L

G

D

D

E

G

E

V

G

D

I

L

N

N

V

T

S

A

K

G

L

L

S

V

A

A

V

V

C

G

G

H

R
|
R

G

-

L

-

R

-

P

V

I

V

E

H

G

G

T

N

Y

T

E

F

V

Y

N

R

E

P

Q

T

M

V

L

L

Q

D

D

D

L

-

R

-

K

-

G

-

Y

-

S

-

G

-

Q

-

H

-

A

-

S

-

N

-

L

-

G

-

G

A

I

V

I

I

A

A

-

R

-

L

-

H

-

E

-

L

N

S

E

E

I

L

A

A

G

P

A

L

L

H

N

N

I

P

P

P

S

A

F

L

I

L

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G

D

I

P

E

V

V

V

A

V

R

D

R

E

L

L

L

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P

D

G

V

I

A

A

R

H

V

V

S

A

G

V

F

F

S

D

-

T

-

G

-

F

-

H

-

D

-

L

-

P

-

A

-

T

-

Y

-

A

-

I

-

D

-

R

-

E

L

L

I

A

E

D

R

R

K

W

S

Q

I

I

-

R

-

Y

-

G

F

F

H
|
H

A

G

L

T

N

S

Q

H

K

R

A

Y

V

V

A

S

R

E

R

Q

V

A

A

A

K

A

E

F

L

L

N

D

K

R

S

P

Y

L

E

R

E

G

L

L

N

K

L

Q

I

I

V

V

T

L

H

H

M

L

G
|
G

G
x
N

G

G

I

C

T

S

V

A

G

S

A

A

H

I

K

A

G

G

G

T

K

R

V

P

I

L

D

D

V

T

N

S

N

M

G

G

L

L

H

T

G

P

-

L

D

E

G

G

P

L

F

V

S

M

P

G

E

T

R
|
R

A

S

G

G

T

D

V

I

P

D

A

P

G

S

D

V

L

V

V

S

D

Y

L

L

C

C

F

H

S

T

G

A

D

G

F

M

Y

G

R

V

E

D

I

V

M

E

K

S

K

M

L

L

V

N

G

H

Q

R

G

S

G

G

L

V

V

V

G

G

Y

L

L

S

G

G

T

V

N

R

D
|
D

A
x
F

I
x
R

Q

R

V

L

E

R

K

E

R

L

I

I

E

E

K

S

G

G

D

D

E

G

K

A

A

A

K

Q

L

L

V

A

Y

Y

D

S

A

V

M

F

A

T

Y

H

Q

R

V

L

A

R

K

K

E

Y

I

I

G

G

S

A

A

Y

S

L

A

A

V

V

L

L

Q

-

G

G

Q

H

V

T

D

D

A

V

I

I

I

S

L

F

T

T

G

A

G
|
G

L
x
I

-

G

-

E

-
x
N

-
x
D

-

A

A

A

Y

V

G

R

K

R

D

D

F

A

V

V

S

S

T

G

I

M

-

E

-

L

-

G

-

I

-

V

-

L

-

D

-

E

-

R

-

N

-

L

-

P

-

G

-

A

-

K

-

G

V

A

Q

R

Q

Q

V

I

S

S

W

-

I

-

A

A

D

D

-

D

-

S

-

P

-

I

-

T

V

V

M

L

V

V

K

V

P

P

G

T

E

N

N
x
E

E

E

L

L

Q

-

A

A

L

I

A

A

E

R

G

D

A

C

L

V

R

R

V

V

L

L

T

G

G

G

active site: N10 (= N11), R74 (= R77), H163 (= H157), R224 (= R217), E367 (≠ N335)
binding diphosphomethylphosphonic acid adenosyl ester: K17 (= K18), G193 (= G187), N194 (≠ G188), D265 (= D258), F266 (≠ A259), R267 (≠ I260), G313 (= G307), I314 (≠ L308), N317 (vs. gap), D318 (vs. gap)

4fwsA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with ctp (see paper)
26% identity, 47% coverage: 148:319/367 of query aligns to 163:335/394 of 4fwsA

query
sites
4fwsA

S

S

L

L

I

G

E

V

R

R

K

R

S

Y

I

G

F

F

H
|
H

A

G

L

T

N

S

Q

H

K

R

A

Y

V

V

A

S

R

R

V

A

A

Y

K

E

E

L

L

L

N

D

K

L

S

D

Y

E

E

K

E

D

L

S

N

G

L

L

I

I

V

V

T

A

H

H

M

L

G
|
G

G
x
N

G
|
G

I

A

T

S

V

I

G

C

A

A

H

V

K

R

G

N

G

G

K

Q

V

S

I

V

D

D

V

T

N

S

N

M

G

G

L

M

H

T

G

P

-

L

D

E

G

G

P

L

F

M

S

M

P

G

E
x
T

R
|
R

A

S

G

G

T

D

V

V

P

D

A

F

G

G

D

A

L

M

V

A

D

W

L

I

C

A

F

K

S

E

G

T

D

G

F

Q

Y

T

R

L

E

S

E

D

I

L

M

E

K

R

K

V

L

V

V

N

G
x
K

Q

E

G

S

G

G

L

L

V

L

G

G

Y

I

L

S

G

G

T

L

N

S

D

S

A
x
D

I
x
L

Q
x
R

V

V

-

L

E

E

K

K

R

A

I

W

E

H

K

E

G

G

D

H

E

E

K

R

A

A

K

R

L

L

V

A

Y

I

D

K

A

T

M

F

A

V

Y

H

Q

R

V

I

A

A

K

R

E

H

I

I

G

A

S

G

A

H

S

A

A

A

V

S

L

L

Q

H

G

-

Q

R

V

L

D

D

A

G

I

I

L

F

T

T

G

G

G
|
G

L
x
I

A

G

Y

E

G
x
N

K

S

D

V

F

L

V

I

S

R

T

Q

I

L

V

V

active site: H172 (= H157), R233 (= R217)
binding cytidine-5'-triphosphate: G202 (= G187), N203 (≠ G188), G204 (= G189), D275 (≠ A259), L276 (≠ I260), R277 (≠ Q261), G323 (= G307), I324 (≠ L308), N327 (≠ G311)
binding 1,2-ethanediol: N203 (≠ G188), T232 (≠ E216), R233 (= R217), K262 (≠ G246)

Sites not aligning to the query:

active site: 8, 83, 378
binding 1,2-ethanediol: 21, 24, 115

4fwrA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with cmp (see paper)
26% identity, 47% coverage: 148:319/367 of query aligns to 163:335/394 of 4fwrA

query
sites
4fwrA

S

S

L

L

I

G

E

V

R

R

K

R

S

Y

I

G

F

F

H
|
H

A

G

L

T

N

S

Q

H

K

R

A

Y

V

V

A

S

R

R

V

A

A

Y

K

E

E

L

L

L

N

D

K

L

S

D

Y

E

E

K

E

D

L

S

N

G

L

L

I

I

V

V

T

A

H

H

M

L

G
|
G

G
x
N

G

G

I

A

T

S

V

I

G

C

A

A

H

V

K

R

G

N

G

G

K

Q

V

S

I

V

D

D

V

T

N

S

N

M

G

G

L

M

H

T

G

P

-

L

D

E

G

G

P

L

F

M

S

M

P

G

E

T

R
|
R

A

S

G

G

T

D

V

V

P

D

A

F

G

G

D

A

L

M

V

A

D

W

L

I

C

A

F

K

S

E

G

T

D

G

F

Q

Y

T

R

L

E

S

E

D

I

L

M

E

K

R

K

V

L

V

V

N

G

K

Q

E

G

S

G

G

L

L

V

L

G

G

Y

I

L

S

G

G

T

L

N

S

D

S

A
x
D

I
x
L

Q
x
R

V

V

-

L

E

E

K

K

R

A

I

W

E

H

K

E

G

G

D

H

E

E

K

R

A

A

K

R

L

L

V

A

Y

I

D

K

A

T

M

F

A

V

Y

H

Q

R

V

I

A

A

K

R

E

H

I

I

G

A

S

G

A

H

S

A

A

A

V

S

L

L

Q

H

G

-

Q

R

V

L

D

D

A

G

I

I

L

F

T

T

G

G

G
|
G

L
x
I

A

G

Y

E

G
x
N

K

S

D

V

F

L

V

I

S

R

T

Q

I

L

V

V

active site: H172 (= H157), R233 (= R217)
binding cytidine-5'-monophosphate: G202 (= G187), N203 (≠ G188), D275 (≠ A259), L276 (≠ I260), R277 (≠ Q261), G323 (= G307), I324 (≠ L308), N327 (≠ G311)

Sites not aligning to the query:

active site: 8, 83, 378

4fwqA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with gtp (see paper)
26% identity, 47% coverage: 148:319/367 of query aligns to 163:335/394 of 4fwqA

query
sites
4fwqA

S

S

L

L

I

G

E

V

R

R

K

R

S

Y

I

G

F

F

H
|
H

A

G

L

T

N

S

Q

H

K

R

A

Y

V

V

A

S

R

R

V

A

A

Y

K

E

E

L

L

L

N

D

K

L

S

D

Y

E

E

K

E

D

L

S

N

G

L

L

I

I

V

V

T

A

H

H

M

L

G

G

G
x
N

G
|
G

I

A

T

S

V

I

G

C

A

A

H

V

K

R

G

N

G

G

K

Q

V

S

I

V

D

D

V

T

N

S

N

M

G

G

L

M

H

T

G

P

-

L

D

E

G

G

P

L

F

M

S

M

P

G

E

T

R
|
R

A

S

G

G

T

D

V

V

P

D

A

F

G

G

D

A

L

M

V

A

D

W

L

I

C

A

F

K

S

E

G

T

D

G

F

Q

Y

T

R

L

E

S

E

D

I

L

M

E

K

R

K

V

L

V

V

N

G

K

Q

E

G

S

G

G

L

L

V

L

G

G

Y

I

L

S

G

G

T

L

N

S

D

S

A
x
D

I
x
L

Q
x
R

V

V

-

L

E
|
E

K

K

R

A

I

W

E

H

K

E

G

G

D

H

E

E

K

R

A

A

K

R

L

L

V

A

Y

I

D

K

A

T

M

F

A

V

Y

H

Q

R

V

I

A

A

K

R

E

H

I

I

G

A

S

G

A

H

S

A

A

A

V

S

L

L

Q

H

G

-

Q

R

V

L

D

D

A

G

I

I

L

F

T

T

G

G

G
|
G

L
x
I

A

G

Y

E

G
x
N

K

S

D

V

F

L

V

I

S

R

T

Q

I

L

V

V

active site: H172 (= H157), R233 (= R217)
binding guanosine-5'-triphosphate: H172 (= H157), N203 (≠ G188), G204 (= G189), D275 (≠ A259), L276 (≠ I260), R277 (≠ Q261), E280 (= E263), G323 (= G307), I324 (≠ L308), N327 (≠ G311)

Sites not aligning to the query:

active site: 8, 83, 378

4fwpA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with gdp (see paper)
26% identity, 47% coverage: 148:319/367 of query aligns to 163:335/394 of 4fwpA

query
sites
4fwpA

S

S

L

L

I

G

E

V

R

R

K

R

S

Y

I

G

F

F

H
|
H

A

G

L

T

N

S

Q

H

K

R

A

Y

V

V

A

S

R

R

V

A

A

Y

K

E

E

L

L

L

N

D

K

L

S

D

Y

E

E

K

E

D

L

S

N

G

L

L

I

I

V

V

T

A

H

H

M

L

G

G

G
x
N

G

G

I

A

T

S

V

I

G

C

A

A

H

V

K

R

G

N

G

G

K

Q

V

S

I

V

D

D

V

T

N

S

N

M

G

G

L

M

H

T

G

P

-

L

D

E

G

G

P

L

F

M

S

M

P

G

E

T

R
|
R

A

S

G

G

T

D

V

V

P

D

A

F

G

G

D

A

L

M

V

A

D

W

L

I

C

A

F

K

S

E

G

T

D

G

F

Q

Y

T

R

L

E

S

E

D

I

L

M

E

K

R

K

V

L

V

V

N

G
x
K

Q

E

G

S

G

G

L

L

V

L

G

G

Y

I

L

S

G

G

T

L

N

S

D

S

A
x
D

I
x
L

Q
x
R

V

V

-

L

E
|
E

K

K

R

A

I

W

E

H

K

E

G

G

D

H

E

E

K

R

A

A

K

R

L

L

V

A

Y

I

D

K

A

T

M

F

A

V

Y

H

Q

R

V

I

A

A

K

R

E

H

I

I

G

A

S

G

A

H

S

A

A

A

V

S

L

L

Q

H

G

-

Q

R

V

L

D

D

A

G

I

I

L

F

T

T

G

G

G
|
G

L
x
I

A

G

Y

E

G
x
N

K
x
S

D

V

F

L

V

I

S

R

T

Q

I

L

V

V

active site: H172 (= H157), R233 (= R217)
binding 1,2-ethanediol: K262 (≠ G246)
binding guanosine-5'-diphosphate: N203 (≠ G188), D275 (≠ A259), L276 (≠ I260), R277 (≠ Q261), E280 (= E263), G323 (= G307), I324 (≠ L308), N327 (≠ G311), S328 (≠ K312)

Sites not aligning to the query:

active site: 8, 83, 378
binding 1,2-ethanediol: 11, 115

4fwoA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with gmp (see paper)
26% identity, 47% coverage: 148:319/367 of query aligns to 163:335/394 of 4fwoA

query
sites
4fwoA

S

S

L

L

I

G

E

V

R

R

K

R

S

Y

I

G

F

F

H
|
H

A

G

L

T

N

S

Q

H

K

R

A

Y

V

V

A

S

R

R

V

A

A

Y

K

E

E

L

L

L

N

D

K

L

S

D

Y

E

E

K

E

D

L

S

N

G

L

L

I

I

V

V

T

A

H

H

M

L

G
|
G

G
x
N

G

G

I

A

T

S

V

I

G

C

A

A

H

V

K

R

G

N

G

G

K

Q

V

S

I

V

D

D

V

T

N

S

N

M

G

G

L

M

H

T

G

P

-

L

D

E

G

G

P

L

F

M

S

M

P

G

E

T

R
|
R

A

S

G

G

T

D

V

V

P

D

A

F

G

G

D

A

L

M

V

A

D

W

L

I

C

A

F

K

S

E

G

T

D

G

F

Q

Y

T

R

L

E

S

E

D

I

L

M

E

K

R

K

V

L

V

V

N

G

K

Q

E

G

S

G

G

L

L

V

L

G

G

Y

I

L

S

G

G

T

L

N

S

D

S

A
x
D

I
x
L

Q
x
R

V

V

-

L

E
|
E

K

K

R

A

I

W

E

H

K

E

G

G

D

H

E

E

K

R

A

A

K

R

L

L

V

A

Y

I

D

K

A

T

M

F

A

V

Y

H

Q

R

V

I

A

A

K

R

E

H

I

I

G

A

S

G

A

H

S

A

A

A

V

S

L

L

Q

H

G

-

Q

R

V

L

D

D

A

G

I

I

L

F

T

T

G

G

G
|
G

L
x
I

A

G

Y

E

G
x
N

K

S

D

V

F

L

V

I

S

R

T

Q

I

L

V

V

active site: H172 (= H157), R233 (= R217)
binding guanosine-5'-monophosphate: G202 (= G187), N203 (≠ G188), D275 (≠ A259), L276 (≠ I260), R277 (≠ Q261), E280 (= E263), G323 (= G307), I324 (≠ L308), N327 (≠ G311)

Sites not aligning to the query:

active site: 8, 83, 378
binding 1,2-ethanediol: 100, 104

4fwnA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with adenosine tetraphosphate (ap4) (see paper)
26% identity, 47% coverage: 148:319/367 of query aligns to 163:335/394 of 4fwnA

query
sites
4fwnA

S

S

L

L

I

G

E

V

R

R

K

R

S

Y

I

G

F

F

H
|
H

A

G

L

T

N

S

Q

H

K

R

A

Y

V

V

A

S

R

R

V

A

A

Y

K

E

E

L

L

L

N

D

K

L

S

D

Y

E

E

K

E

D

L

S

N

G

L

L

I

I

V

V

T

A

H
|
H

M

L

G

G

G
x
N

G
|
G

I

A

T

S

V

I

G

C

A

A

H

V

K

R

G

N

G

G

K

Q

V

S

I

V

D

D

V

T

N

S

N

M

G

G

L

M

H

T

G

P

-

L

D

E

G

G

P

L

F

M

S

M

P

G

E

T

R
|
R

A

S

G

G

T

D

V

V

P

D

A

F

G

G

D

A

L

M

V

A

D

W

L

I

C

A

F

K

S

E

G

T

D

G

F

Q

Y

T

R

L

E

S

E

D

I

L

M

E

K

R

K

V

L

V

V

N

G

K

Q

E

G

S

G

G

L

L

V

L

G

G

Y

I

L

S

G

G

T

L

N

S

D

S

A
x
D

I
x
L

Q
x
R

V

V

-

L

E

E

K

K

R

A

I

W

E

H

K

E

G

G

D

H

E

E

K

R

A

A

K

R

L

L

V

A

Y

I

D

K

A

T

M

F

A

V

Y

H

Q

R

V

I

A

A

K

R

E

H

I

I

G

A

S

G

A

H

S

A

A

A

V

S

L

L

Q

H

G

-

Q

R

V

L

D

D

A

G

I

I

L

F

T

T

G

G

G
|
G

L
x
I

A

G

Y

E

G
x
N

K

S

D

V

F

L

V

I

S

R

T

Q

I

L

V

V

active site: H172 (= H157), R233 (= R217)
binding adenosine-5'-tetraphosphate: H172 (= H157), H200 (= H185), N203 (≠ G188), G204 (= G189), D275 (≠ A259), L276 (≠ I260), R277 (≠ Q261), G323 (= G307), I324 (≠ L308), N327 (≠ G311)

Sites not aligning to the query:

active site: 8, 83, 378

4fwmA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with atp (see paper)
26% identity, 47% coverage: 148:319/367 of query aligns to 163:335/394 of 4fwmA

query
sites
4fwmA

S

S

L

L

I

G

E

V

R

R

K

R

S

Y

I

G

F

F

H
|
H

A

G

L

T

N

S

Q

H

K

R

A

Y

V

V

A

S

R

R

V

A

A

Y

K

E

E

L

L

L

N

D

K

L

S

D

Y

E

E

K

E

D

L

S

N

G

L

L

I

I

V

V

T

A

H
|
H

M

L

G

G

G
x
N

G
|
G

I

A

T

S

V

I

G

C

A

A

H

V

K

R

G

N

G

G

K

Q

V

S

I

V

D

D

V

T

N

S

N

M

G

G

L

M

H

T

G

P

-

L

D

E

G

G

P

L

F

M

S

M

P

G

E

T

R
|
R

A

S

G

G

T

D

V

V

P

D

A

F

G

G

D

A

L

M

V

A

D

W

L

I

C

A

F

K

S

E

G

T

D

G

F

Q

Y

T

R

L

E

S

E

D

I

L

M

E

K

R

K

V

L

V

V

N

G

K

Q

E

G

S

G

G

L

L

V

L

G

G

Y

I

L

S

G

G

T

L

N

S

D

S

A
x
D

I
x
L

Q
x
R

V

V

-

L

E

E

K

K

R

A

I

W

E

H

K

E

G

G

D

H

E

E

K

R

A

A

K

R

L

L

V

A

Y

I

D

K

A

T

M

F

A

V

Y

H

Q

R

V

I

A

A

K

R

E

H

I

I

G

A

S

G

A

H

S

A

A

A

V

S

L

L

Q

H

G

-

Q

R

V

L

D

D

A

G

I

I

L

F

T

T

G

G

G
|
G

L
x
I

A

G

Y

E

G
x
N

K

S

D

V

F

L

V

I

S

R

T

Q

I

L

V

V

active site: H172 (= H157), R233 (= R217)
binding adenosine-5'-triphosphate: H172 (= H157), H200 (= H185), N203 (≠ G188), G204 (= G189), D275 (≠ A259), L276 (≠ I260), R277 (≠ Q261), G323 (= G307), I324 (≠ L308), N327 (≠ G311)
binding 1,2-ethanediol: H172 (= H157), R233 (= R217)

Sites not aligning to the query:

active site: 8, 83, 378

4fwkA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with amp (see paper)
26% identity, 47% coverage: 148:319/367 of query aligns to 163:335/394 of 4fwkA

query
sites
4fwkA

S

S

L

L

I

G

E

V

R

R

K

R

S

Y

I

G

F

F

H
|
H

A

G

L

T

N

S

Q

H

K

R

A

Y

V

V

A

S

R

R

V

A

A

Y

K

E

E

L

L

L

N

D

K

L

S

D

Y

E

E

K

E

D

L

S

N

G

L

L

I

I

V

V

T

A

H

H

M

L

G
|
G

G
x
N

G

G

I

A

T

S

V

I

G

C

A

A

H

V

K

R

G

N

G

G

K

Q

V

S

I

V

D

D

V

T

N

S

N

M

G

G

L

M

H

T

G

P

-

L

D

E

G

G

P

L

F

M

S

M

P

G

E

T

R
|
R

A

S

G

G

T

D

V

V

P

D

A

F

G

G

D

A

L

M

V

A

D

W

L

I

C

A

F

K

S

E

G

T

D

G

F

Q

Y

T

R

L

E

S

E

D

I

L

M

E

K

R

K

V

L

V

V

N

G

K

Q

E

G

S

G

G

L

L

V

L

G

G

Y

I

L

S

G

G

T

L

N

S

D

S

A
x
D

I
x
L

Q
x
R

V

V

-

L

E

E

K

K

R

A

I

W

E

H

K

E

G

G

D

H

E

E

K

R

A

A

K

R

L

L

V

A

Y

I

D

K

A

T

M

F

A

V

Y

H

Q

R

V

I

A

A

K

R

E

H

I

I

G

A

S

G

A

H

S

A

A

A

V

S

L

L

Q

H

G

-

Q

R

V

L

D

D

A

G

I

I

L

F

T

T

G

G

G
|
G

L
x
I

A

G

Y

E

G
x
N

K

S

D

V

F

L

V

I

S

R

T

Q

I

L

V

V

active site: H172 (= H157), R233 (= R217)
binding adenosine monophosphate: G202 (= G187), N203 (≠ G188), D275 (≠ A259), L276 (≠ I260), R277 (≠ Q261), G323 (= G307), I324 (≠ L308), N327 (≠ G311)

Sites not aligning to the query:

active site: 8, 83, 378
binding 1,2-ethanediol: 103, 104, 107

2e1zA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with diadenosine tetraphosphate (ap4a) obtained after co- crystallization with atp (see paper)
26% identity, 47% coverage: 148:319/367 of query aligns to 163:335/394 of 2e1zA

query
sites
2e1zA

S

S

L

L

I

G

E

V

R

R

K

R

S

Y

I

G

F

F

H
|
H

A

G

L

T

N

S

Q

H

K

R

A

Y

V

V

A

S

R

R

V

A

A

Y

K

E

E

L

L

L

N

D

K

L

S

D

Y

E

E

K

E

D

L

S

N

G

L

L

I

I

V

V

T

A

H

H

M

L

G
|
G

G
x
N

G
|
G

I

A

T

S

V

I

G

C

A

A

H

V

K

R

G

N

G

G

K

Q

V

S

I

V

D

D

V

T

N

S

N

M

G

G

L

M

H

T

G
x
P

-

L

D

E

G

G

P

L

F

M

S

M

P

G

E

T

R
|
R

A

S

G

G

T

D

V

V

P

D

A

F

G

G

D

A

L

M

V

A

D

W

L

I

C

A

F

K

S

E

G

T

D

G

F

Q

Y

T

R

L

E

S

E

D

I

L

M

E

K

R

K

V

L

V

V

N

G

K

Q

E

G

S

G

G

L

L

V

L

G

G

Y

I

L

S

G

G

T

L

N

S

D

S

A
x
D

I
x
L

Q
x
R

V

V

-

L

E

E

K

K

R

A

I

W

E

H

K

E

G

G

D

H

E

E

K

R

A

A

K

R

L

L

V

A

Y

I

D

K

A

T

M

F

A

V

Y

H

Q

R

V

I

A

A

K

R

E

H

I

I

G

A

S

G

A

H

S

A

A

A

V

S

L

L

Q

H

G

-

Q

R

V

L

D

D

A

G

I

I

L

F

T

T

G

G

G
|
G

L
x
I

A

G

Y

E

G
x
N

K

S

D

V

F

L

V

I

S

R

T

Q

I

L

V

V

active site: H172 (= H157), R233 (= R217)
binding bis(adenosine)-5'-tetraphosphate: G202 (= G187), N203 (≠ G188), G204 (= G189), P224 (≠ G209), R233 (= R217), D275 (≠ A259), L276 (≠ I260), R277 (≠ Q261), G323 (= G307), I324 (≠ L308), N327 (≠ G311)

Sites not aligning to the query:

active site: 8, 83, 378
binding bis(adenosine)-5'-tetraphosphate: 8, 83, 115

1x3nA Crystal structure of amppnp bound propionate kinase (tdcd) from salmonella typhimurium (see paper)
26% identity, 47% coverage: 148:319/367 of query aligns to 163:335/394 of 1x3nA

query
sites
1x3nA

S

S

L

L

I

G

E

V

R

R

K

R

S

Y

I

G

F

F

H
|
H

A

G

L

T

N

S

Q

H

K

R

A

Y

V

V

A

S

R

R

V

A

A

Y

K

E

E

L

L

L

N

D

K

L

S

D

Y

E

E

K

E

D

L

S

N

G

L

L

I

I

V

V

T

A

H

H

M

L

G
|
G

G
x
N

G
|
G

I

A

T

S

V

I

G

C

A

A

H

V

K

R

G

N

G

G

K

Q

V

S

I

V

D

D

V

T

N

S

N

M

G

G

L

M

H

T

G

P

-

L

D

E

G

G

P

L

F

M

S

M

P

G

E

T

R
|
R

A

S

G

G

T

D

V

V

P

D

A

F

G

G

D

A

L

M

V

A

D

W

L

I

C

A

F

K

S

E

G

T

D

G

F

Q

Y

T

R

L

E

S

E

D

I

L

M

E

K

R

K

V

L

V

V

N

G

K

Q

E

G

S

G

G

L

L

V

L

G

G

Y

I

L

S

G

G

T

L

N

S

D

S

A
x
D

I
x
L

Q
x
R

V

V

-

L

E

E

K

K

R

A

I

W

E

H

K

E

G

G

D

H

E

E

K

R

A

A

K

R

L

L

V

A

Y

I

D

K

A

T

M

F

A

V

Y

H

Q

R

V

I

A

A

K

R

E

H

I

I

G

A

S

G

A

H

S

A

A

A

V

S

L

L

Q

H

G

-

Q

R

V

L

D

D

A

G

I

I

L

F

T

T

G

G

G
|
G

L
x
I

A

G

Y

E

G
x
N

K

S

D

V

F

L

V

I

S

R

T

Q

I

L

V

V

active site: H172 (= H157), R233 (= R217)
binding phosphoaminophosphonic acid-adenylate ester: G202 (= G187), N203 (≠ G188), G204 (= G189), D275 (≠ A259), L276 (≠ I260), R277 (≠ Q261), G323 (= G307), I324 (≠ L308), N327 (≠ G311)

Sites not aligning to the query:

active site: 8, 83, 378

1x3mA Crystal structure of adp bound propionate kinase (tdcd) from salmonella typhimurium (see paper)
26% identity, 47% coverage: 148:319/367 of query aligns to 163:335/394 of 1x3mA

query
sites
1x3mA

S

S

L

L

I

G

E

V

R

R

K

R

S

Y

I

G

F

F

H
|
H

A

G

L

T

N

S

Q

H

K

R

A

Y

V

V

A

S

R

R

V

A

A

Y

K

E

E

L

L

L

N

D

K

L

S

D

Y

E

E

K

E

D

L

S

N

G

L

L

I

I

V

V

T

A

H

H

M

L

G
|
G

G
x
N

G

G

I

A

T

S

V

I

G

C

A

A

H

V

K

R

G

N

G

G

K

Q

V

S

I

V

D

D

V

T

N

S

N

M

G

G

L

M

H

T

G

P

-

L

D

E

G

G

P

L

F

M

S

M

P

G

E

T

R
|
R

A

S

G

G

T

D

V

V

P

D

A

F

G

G

D

A

L

M

V

A

D

W

L

I

C

A

F

K

S

E

G

T

D

G

F

Q

Y

T

R

L

E

S

E

D

I

L

M

E

K

R

K

V

L

V

V

N

G

K

Q

E

G

S

G

G

L

L

V

L

G

G

Y

I

L

S

G

G

T

L

N

S

D

S

A
x
D

I
x
L

Q
x
R

V

V

-

L

E

E

K

K

R

A

I

W

E

H

K

E

G

G

D

H

E

E

K

R

A

A

K

R

L

L

V

A

Y

I

D

K

A

T

M

F

A

V

Y

H

Q

R

V

I

A

A

K

R

E

H

I

I

G

A

S

G

A

H

S

A

A

A

V

S

L

L

Q

H

G

-

Q

R

V

L

D

D

A

G

I

I

L

F

T

T

G
|
G

L
x
I

A

G

Y

E

G
x
N

K

S

D

V

F

L

V

I

S

R

T

Q

I

L

V

V

active site: H172 (= H157), R233 (= R217)
binding adenosine-5'-diphosphate: G202 (= G187), N203 (≠ G188), D275 (≠ A259), L276 (≠ I260), R277 (≠ Q261), G322 (= G306), G323 (= G307), I324 (≠ L308), N327 (≠ G311)

Sites not aligning to the query:

active site: 8, 83, 378

9dv9A The amp-bound structure of acka from treponema vincentii (see paper)
26% identity, 56% coverage: 152:357/367 of query aligns to 179:386/453 of 9dv9A

query
sites
9dv9A

R

R

K

R

S

Y

I

G

F

F

H

H

A

G

L

T

N

S

Q

F

K

L

A

Y

V

T

A

A

R

K

R

R

V

A

A

A

K

V

E

I

L

L

N

G

K

K

S

K

Y

P

E

E

E

D

L

T

N

N

L

I

I

I

V

I

T

A

H

H

M

I

G
|
G

G

N

G

G

I

A

T

S

V

M

G

C

A

C

H

V

K

K

G

Q

G

G

K

K

V

C

I

F

D

D

V

T

N

S

N

M

G

G

L

L

H

T

G

P

-

L

D

E

G

G

P

L

F

V

S

M

P

G

E

T

R

R

A

S

G

G

T

D

V

C

-

D

P

P

A

A

G

L

D

P

L

F

V

Y

D

I

L

M

C

R

F

K

S

T

G

G

D

-

F

M

Y

T

R

P

E

A

E

E

I

M

M

D

K

T

K

A

L

L

V

N

G

K

Q

K

G

S

G

G

L

L

V

L

G

G

Y

V

L

T

G

G

T

Q

N

Y

-

V

D
|
D

A
x
R

I
x
R

Q

D

V

V

E

S

K

K

R

A

I

M

E

G

K

E

G

G

D

D

E

K

K

R

A

A

K

R

L

L

V

A

Y

F

D

N

A

M

M

E

A

V

Y

Y

Q

R

V

L

A

Q

K

K

E

Y

I

F

G

G

S

A

A

Y

S

I

A

A

V

A

L

L

Q

G

G

Q

Q

K

V

P

D

D

A

A

I

I

I

V

L

F

T

T

G
x
A

G
|
G

L

V

A

G

-

E

Y
x
F

G

G

K

F

D

D

F

T

V

R

S

L

T

A

I

V

V

C

Q

E

Q

G

V

L

S

T

W

H

I

L

A

G

D

-

V

I

M

K

V

I

K

D

P

P

G

K

E

K

N

N

E

A

L

L

Q

A

A

R

L

T

A

R

E

N

G

A

A

E

L

T

R

C

V

I

L

S

T

A

G

D

E

D

E

S

K

P

A

V

K

K

V

I

Y

F

binding adenosine monophosphate: G214 (= G187), D287 (= D258), R288 (≠ A259), R289 (≠ I260), A335 (≠ G306), G336 (= G307), F340 (≠ Y310)

7fj9A Kpacka (pduw) with amppnp complex structure
28% identity, 43% coverage: 152:308/367 of query aligns to 173:330/395 of 7fj9A

query
sites
7fj9A

R

R

K

R

S

Y

I

G

F

F

H
|
H

A

G

L

T

N

S

Q

H

K

H

A

Y

V

V

A

S

R

S

R

A

V

L

A

A

K

E

E

K

L

L

N

G

K

V

S

P

Y

L

E

S

E

A

L

L

N

R

L

V

I

V

V

S

T

C

H

H

M

L

G
|
G

G
x
N

G
|
G

I

C

T

S

V

V

G

C

A

A

H

I

K

K

G

G

K

Q

V

S

I

V

D

N

V

T

N

S

N

M

G

G

L

-

H

-

G

-

D

-

G

-

P

-

F

F

S

T

P

P

E

Q

-

S

-

G

-

V

-

M

-

G

-

T

R

R

A

S

G

G

T

D

V

I

P

D

A

P

G

S

D

I

L

L

V

P

D

W

L

L

C

V

F

E

S

K

G

E

D

G

F

K

Y

S

R

A

E

Q

I

L

M

S

K

Q

K

L

L

L

V

N

G

N

Q

E

G

S

G

G

L

L

V

L

G

G

Y

V

L

S

G

G

-

V

T

S

N

S

D
|
D

A
x
Y

I
x
R

Q

D

V

V

E

E

K

Q

R

A

I

A

E

D

K

A

G

G

D

N

E

E

K

R

A

A

K

A

L

L

V

A

Y

L

D

S

A

L

M

F

A

A

Y

E

Q

R

V

I

A

R

K

A

E

T

I

I

G

G

S

-

A

-

S

S

A

Y

V

I

L

M

Q

Q

-

M

G

G

Q

G

V

L

D

D

A

A

I

L

I

I

L

F

T

T

G

G

G
|
G

L
x
I

binding phosphoaminophosphonic acid-adenylate ester: H178 (= H157), G208 (= G187), N209 (≠ G188), G210 (= G189), D281 (= D258), Y282 (≠ A259), R283 (≠ I260), G329 (= G307), I330 (≠ L308)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 333

7fj8A Kpacka (pduw) with amp complex structure
28% identity, 43% coverage: 152:308/367 of query aligns to 173:330/395 of 7fj8A

query
sites
7fj8A

R

R

K

R

S

Y

I

G

F

F

H

H

A

G

L

T

N

S

Q

H

K

H

A

Y

V

V

A

S

R

S

R

A

V

L

A

A

K

E

E

K

L

L

N

G

K

V

S

P

Y

L

E

S

E

A

L

L

N

R

L

V

I

V

V

S

T

C

H

H

M

L

G
|
G

G
x
N

G

G

I

C

T

S

V

V

G

C

A

A

H

I

K

K

G

G

K

Q

V

S

I

V

D

N

V

T

N

S

N

M

G

G

L

-

H

-

G

-

D

-

G

-

P

-

F

F

S

T

P

P

E

Q

-

S

-

G

-

V

-

M

-

G

-

T

R

R

A

S

G

G

T

D

V

I

P

D

A

P

G

S

D

I

L

L

V

P

D

W

L

L

C

V

F

E

S

K

G

E

D

G

F

K

Y

S

R

A

E

Q

I

L

M

S

K

Q

K

L

L

L

V

N

G

N

Q

E

G

S

G

G

L

L

V

L

G

G

Y

V

L

S

G

G

-

V

T

S

N

S

D
|
D

A
x
Y

I
x
R

Q

D

V

V

E

E

K

Q

R

A

I

A

E

D

K

A

G

G

D

N

E

E

K

R

A

A

K

A

L

L

V

A

Y

L

D

S

A

L

M

F

A

A

Y

E

Q

R

V

I

A

R

K

A

E

T

I

I

G

G

S

-

A

-

S

S

A

Y

V

I

L

M

Q

Q

-

M

G

G

Q

G

V

L

D

D

A

A

I

L

I

I

L

F

T

T

G

G

G
|
G

L
x
I

binding adenosine monophosphate: G208 (= G187), N209 (≠ G188), D281 (= D258), Y282 (≠ A259), R283 (≠ I260), G329 (= G307), I330 (≠ L308)

Sites not aligning to the query:

binding adenosine monophosphate: 333

Query Sequence

>WP_010173582.1 NCBI__GCF_000171615.1:WP_010173582.1
MQEQFRILVINPGSTSTKIGVFDNDDVVFEKTLRHDSDEINQYPSIIDQYEFRKTTILHA
LDEEGINVSKLSAVCGRGGLLRPIEGGTYEVNEQMLQDLRKGYSGQHASNLGGIIANEIA
GALNIPSFIVDPVVVDELSDVARVSGFSLIERKSIFHALNQKAVARRVAKELNKSYEELN
LIVTHMGGGITVGAHKGGKVIDVNNGLHGDGPFSPERAGTVPAGDLVDLCFSGDFYREEI
MKKLVGQGGLVGYLGTNDAIQVEKRIEKGDEKAKLVYDAMAYQVAKEIGSASAVLQGQVD
AIILTGGLAYGKDFVSTIVQQVSWIADVMVKPGENELQALAEGALRVLTGEEKAKVYPNQ
VKTGLNV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory