SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_010177305.1 NCBI__GCF_000255455.1:WP_010177305.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P51648 Aldehyde dehydrogenase family 3 member A2; Aldehyde dehydrogenase 10; Fatty aldehyde dehydrogenase; Microsomal aldehyde dehydrogenase; EC 1.2.1.3; EC 1.2.1.94 from Homo sapiens (Human) (see 5 papers)
44% identity, 96% coverage: 15:462/469 of query aligns to 1:448/485 of P51648

query
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P51648

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I45 (= I63) to F: in SLS; severe loss of activity
V64 (≠ L82) to D: in SLS; severe loss of activity; dbSNP:rs72547556
L106 (= L124) to R: in SLS; severe loss of activity; dbSNP:rs72547558
N112 (= N130) mutation to A: Loss of enzyme activity.
P114 (= P132) to L: in SLS; severe loss of activity; dbSNP:rs72547559
P121 (= P139) to L: in SLS; severe loss of activity; dbSNP:rs72547560
T184 (= T202) to M: in SLS; severe loss of activity; dbSNP:rs72547562; to R: in SLS; severe loss of activity
G185 (= G203) to A: in SLS; severe loss of activity; dbSNP:rs72547563
E207 (= E225) active site; mutation to Q: Loss of enzyme activity.
C214 (≠ A232) to Y: in SLS; 4% of activity; dbSNP:rs72547564
R228 (= R246) to C: in SLS; severe loss of activity; dbSNP:rs72547566
C237 (≠ A255) to Y: in SLS; severe loss of activity; dbSNP:rs72547567
C241 (= C259) active site; mutation to S: Loss of enzyme activity.
D245 (= D263) to N: in SLS; severe loss of activity; dbSNP:rs72547568
K266 (≠ A281) to N: in SLS; mild reduction of activity; the underlying nucleotide substitution affects transcript stability; dbSNP:rs72547569
Y279 (≠ F295) to N: in SLS; severe loss of activity; dbSNP:rs72547570
AP 314:315 (≠ EP 330:331) natural variant: AP -> GAKSTVGA (in SLS; 8% of activity)
P315 (= P331) to S: in SLS; common mutation in Europeans; severe loss of enzymatic activity; dbSNP:rs72547571
E331 (= E347) mutation to Q: Loss of enzyme activity.
S365 (= S381) to L: in SLS; severe loss of activity; dbSNP:rs72547573
Y410 (= Y426) mutation to F: Decreased enzyme activity with dodecanal and hexadecanal. No effect on enzyme activity with octanal.
H411 (= H427) to Y: in SLS; severe loss of activity
S415 (= S431) to N: in SLS; severe loss of activity
F419 (= F435) to S: in SLS; severe loss of activity; dbSNP:rs72547576
R423 (≠ K439) to H: in SLS; severe loss of activity; dbSNP:rs768290318
K447 (= K461) to E: in SLS; severe loss of activity; dbSNP:rs67939114

Sites not aligning to the query:

445:485 mutation Missing: Decreased enzyme activity with dodecanal. Strongly decreased enzyme activity with hexadecanal. No effect on enzyme activity with octanal.

P30838 Aldehyde dehydrogenase, dimeric NADP-preferring; ALDHIII; Aldehyde dehydrogenase 3; Aldehyde dehydrogenase family 3 member A1; EC 1.2.1.5 from Homo sapiens (Human) (see 4 papers)
43% identity, 95% coverage: 17:462/469 of query aligns to 2:450/453 of P30838

query
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P30838

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S134 (≠ T149) to A: in dbSNP:rs887241
E210 (= E225) active site
C244 (= C259) active site; mutation to S: Abolishes activity.
P329 (≠ A342) to A: in allele ALDH3A1*2; dbSNP:rs2228100

4l1oB Crystal structure of human aldh3a1 with inhibitor 1-{[4-(1,3- benzodioxol-5-ylmethyl)piperazin-1-yl]methyl}-1h-indole-2,3-dione (see paper)
43% identity, 95% coverage: 17:462/469 of query aligns to 1:449/452 of 4l1oB

query
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E

N

K

Q

F

I

L

V

T

E

Q

K

V

L

V

K

A

K

E

S

I

L

K

K

K

E

-

F

-

Y

Q

G

N

E

Y

D

S

A

I

K

E

K

N

S

G

R

N

D

F

Y

V

G

E

R

L

I

I

I

N

S

D

A

K

R

N

H

F

F

D

Q

R

R

I

V

V

M

S

G

L

L

I

I

D

E

S

G

Q

Q

K

K

V

V

Y

A

Y

Y

G

G

N

T

Y

G

I

D

K

A

E

A

T

T

R

R

F

Y

I

I

E

A

P

P

T

T

I

I

L

L

T

T

N

D

V

V

S

D

V

P

D

Q

D

S

A

P

V

V

M

M

Q

Q

E

E

E
|
E

I

I

F

F

G

G

P

P

V

V

L

L

P

P

V

I

I

V

E

C

Y

V

D

R

T

S

I

L

D

E

E

E

A

A

F

I

A

Q

I

F

I

I

Q

N

K

Q

F

R

E

E

K

K

P

P

L

L

S

A

A

L

Y

Y

L

M

F

F

S

S

N

S

N

N

S

D

T

K

I

V

K

I

K

K

R

K

F

M

L

I

N

A

E

E

V

T

S

S

F

S

G

G

N

G

G

V

A

A

I

A

N

N

D

D

S

V

I
|
I

M

V

Q

H

F
x
I

T

T

N

L

H

H

N

S

L

L

P

P

F
|
F

G

G

V

V

G

G

N

N

S

S

G

G

T

M

G

G

S

S

Y
|
Y

H
|
H

G

G

K

K

F

K

S

S

F

F

D

E

C

T

F

F

S

S

H

H

A

R

K

R

S

S

I

C

L

L

S

V

K

R

S

P

T

L

W

M

I

N

E

D

A

E

N

G

L

L

K

K

Y

V

-

R

Y

Y

E

P

H

P

T

S

D

P

T

A

K

K

M

M

active site: N114 (= N130), K137 (= K153), E209 (= E225), C243 (= C259), E333 (= E347), Y412 (= Y426)
binding (3S)-1-{[4-(1,3-benzodioxol-5-ylmethyl)piperazin-1-yl]methyl}-3-hydroxy-1,3-dihydro-2H-indol-2-one: Y115 (≠ F131), N118 (= N134), L119 (≠ I135), E209 (= E225), T242 (≠ I258), C243 (= C259), I391 (= I405), I394 (≠ F408), F401 (= F415), H413 (= H427)

8bb8A Crystal structure of human aldehyde dehydrogenase aldh3a1 in complex with octanal (see paper)
43% identity, 93% coverage: 17:453/469 of query aligns to 1:439/447 of 8bb8A

query
sites
8bb8A

T

S

E

K

V

I

K

S

A

E

L

A

I

V

D

K

T

R

Q

A

R

R

K

A

F

A

F

F

Y

S

S

S

N

G

A

R

T

T

K

R

P

P

V

L

S

Q

F

F

R

R

I

I

A

Q

Q

Q

L

L

K

E

K

A

L

L

K

Q

Q

R

L

L

L

I

K

Q

D

E

N

Q

E

E

A

Q

Q

E

L

L

H

V

K

G

A

A

I

L

Y

A

A

A

D

D

L

L

R

H

K

K

S

N

E

E

F

W

D

N

N

A

L

Y

T

Y

T

E

E

E

L

V

P
x
Y

L

V

Y

L

S

E

E

E

I

I

D

E

Y

Y

A

M

V

I

K

Q

N

K

L

L

K

P

K

E

W

W

A

A

T

A

P

D

K

E

R

P

V

V

K

E

T

K

T

T

I

P

L

Q

N

T

F

Q

P

Q

S

D

K

E

S

L

Y

Y

I

I

V

H

A

S

E

E

P

P

L

L

G

G

V

V

S

V

L

L

I

V

I

I

G

G

A

T

W

W

N

N

F
x
Y

P

P

Y

F

N
|
N

I

L

A

T

L

I

I

Q

P

P

I

M

V

V

G

G

A

A

I

I

A

A

G

G

N

N

T

S

S

V

I

V

L

L

K

K

P

P

S

S

E

E

M

L

A

S

P

E

A

N

T

M

S

A

A

S

I

L

M

L

A

A

E

T

L

I

I

I

N

P

K

Q

N

Y

F

L

P

D

S

K

N

D

Y

L

L

Y

K

P

V

V

L

I

Q

N

G

G

I

V

P

P

E

E

T

T

A

E

I

L

L

L

K

K

Q

E

R

R

F

F

D

D

K

H

I

I

F

L

F

Y

T

T

G

G

S

S

P

T

Q

G

V

V

G

G

K

K

I

I

V

I

N

M

Q

T

A

A

P

K

H

H

L

L

T

T

N

P

V

V

T

T

L

L

E

E

L

L

G

G

K

K

N

S

P

P

A

C

I

Y

F

V

T

D

K

K

D

N

C

C

S

D

L

L

N

D

I

V

G

A

V

C

K

R

R

R

M

I

V

A

S

W

G

G

K

K

F

F

L

M

N

N

A

S

G

G

Q

Q

I

T

C
|
C

I

V

T

A

S

P

D

D

Y

Y

V

I

L

L

V

C

Q

D

K

P

D

S

I

I

K

Q

E

N

K

Q

F

I

L

V

T

E

Q

K

V

L

V

K

A

K

E

S

I

L

K

K

K

E

-

F

-

Y

Q

G

N

E

Y

D

S

A

I

K

E

K

N

S

G

R

N

D

F

Y

V

G

E

R

L

I

I

I

N

S

D

A

K

R

N

H

F

F

D

Q

R

R

I

V

V

M

S

G

L

L

I

I

D

E

S

G

Q

Q

K

K

V

V

Y

A

Y

Y

G

G

N

T

Y

G

I

D

K

A

E

A

T

T

R

R

F

Y

I

I

E

A

P

P

T

T

I

I

L

L

T

T

N

D

V

V

S

D

V

P

D

Q

D

S

A

P

V

V

M

M

Q

Q

E

E

I

I

F

F

G

G

P

P

V

V

L

L

P

P

V

I

I

V

E

C

Y

V

D

R

T

S

I

L

D

E

E

E

A

A

F

I

A

Q

I

F

I

I

Q

N

K

Q

F

R

E

E

K

K

P

P

L

L

S

A

A

L

Y

Y

L

M

F

F

S

S

N

S

N

N

S

D

T

K

I

V

K

I

K

K

R

K

F

M

L

I

N

A

E

E

V

T

S

S

F

S

G

G

N

G

G

V

A

A

I

A

N

N

D

D

S

V

I

I

M

V

Q

H

F
x
I

T
|
T

N

L

H

H

N

S

L

L

P

P

F

F

G

G

V

V

G

G

N

N

S

S

G

G

T

M

G

G

S

S

Y

Y

H
|
H

G

G

K

K

F

K

S

S

F

F

D

E

C

T

F

F

S

S

H

H

A

R

K

R

S

S

I

C

L

L

S

V

K

R

S

P

T

L

W

M

I

N

E

D

A

E

N

G

L

L

K

K

binding octanal: Y65 (≠ P81), Y115 (≠ F131), N118 (= N134), C243 (= C259), I394 (≠ F408), T395 (= T409), H413 (= H427)

3szbA Crystal structure of human aldh3a1 modified with the beta-elimination product of aldi-1; 1-phenyl- 2-propen-1-one (see paper)
43% identity, 93% coverage: 17:453/469 of query aligns to 1:439/447 of 3szbA

query
sites
3szbA

T

S

E

K

V

I

K

S

A

E

L

A

I

V

D

K

T

R

Q

A

R

R

K

A

F

A

F

F

Y

S

S

S

N

G

A

R

T

T

K

R

P

P

V

L

S

Q

F

F

R

R

I

I

A

Q

Q

Q

L

L

K

E

K

A

L

L

K

Q

Q

R

L

L

L

I

K

Q

D

E

N

Q

E

E

A

Q

Q

E

L

L

H

V

K

G

A

A

I

L

Y

A

A

A

D

D

L

L

R

H

K

K

S

N

E

E

F

W

D

N

N

A

L

Y

T

Y

T

E

E

E

L

V

P

Y

L

V

Y

L

S

E

E

E

I

I

D

E

Y

Y

A

M

V

I

K

Q

N

K

L

L

K

P

K

E

W

W

A

A

T

A

P

D

K

E

R

P

V

V

K

E

T

K

T

T

I

P

L

Q

N

T

F

Q

P

Q

S

D

K

E

S

L

Y

Y

I

I

V

H

A

S

E

E

P

P

L

L

G

G

V

V

S

V

L

L

I

V

I

I

G

G

A

T

W

W

N
|
N

F
x
Y

P

P

Y

F

N

N

I
x
L

A

T

L

I

I

Q

P

P

I

M

V

V

G

G

A

A

I

I

A

A

G

G

N

N

T

A

S

V

I

V

L

L

K
|
K

P

P

S

S

E

E

M

L

A

S

P

E

A

N

T

M

S

A

A

S

I

L

M

L

A

A

E

T

L

I

I

I

N

P

K

Q

N

Y

F

L

P

D

S

K

N

D

Y

L

L

Y

K

P

V

V

L

I

Q

N

G

G

I

V

P

P

E

E

T

T

A

E

I

L

L

L

K

K

Q

E

R

R

F

F

D

D

K

H

I

I

F

L

F

Y

T

T

G

G

S

S

P

T

Q

G

V

V

G

G

K

K

I

I

V

I

N

M

Q

T

A

A

P

K

H

H

L

L

T

T

N

P

V

V

T

T

L

L

E
|
E

L

L

G

G

K

K

N

S

P

P

A

C

I

Y

F

V

T

D

K

K

D

N

C

C

S

D

L

L

N

D

I

V

G

A

V

C

K

R

R

R

M

I

V

A

S

W

G

G

K

K

F

F

L

M

N

N

A

S

G

G

Q

Q

I

T

C
|
C

I

V

T

A

S

P

D

D

Y

Y

V

I

L

L

V

C

Q

D

K

P

D

S

I

I

K

Q

E

-

K

-

F

-

L

-

T

N

Q

Q

V

I

V

V

A

E

E

K

I

L

K

K

-

S

-

L

Q

K

N

E

Y

F

S

Y

I

G

E

E

N

D

G

A

-

K

-

S

-

R

N

D

F

Y

V

G

E

R

L

I

I

I

N

S

D

A

K

R

N

H

F

F

D

Q

R

R

I

V

V

M

S

G

L

L

I

I

D

E

S

G

Q

Q

K

K

V

V

Y

A

Y

Y

G

G

N

T

Y

G

I

D

K

A

E

A

T

T

R

R

F

Y

I

I

E

A

P

P

T

T

I

I

L

L

T

T

N

D

V

V

S

D

V

P

D

Q

D

S

A

P

V

V

M

M

Q

Q

E

E

E
|
E

I

I

F

F

G

G

P

P

V

V

L

L

P

P

V

I

I

V

E

C

Y

V

D

R

T

S

I

L

D

E

E

E

A

A

F

I

A

Q

I

F

I

I

Q

N

K

Q

F

R

E

E

K

K

P

P

L

L

S

A

A

L

Y

Y

L

M

F

F

S

S

N

S

N

N

S

D

T

K

I

V

K

I

K

K

R

K

F

M

L

I

N

A

E

E

V

T

S

S

F

S

G

G

N

G

G

V

A

A

I

A

N

N

D

D

S

V

I

I

M

V

Q

H

F
x
I

T

T

N

L

H

H

N

S

L

L

P

P

F

F

G

G

V

V

G

G

N

N

S

S

G

G

T

M

G

G

S

S

Y
|
Y

H

H

G

G

K

K

F

K

S

S

F

F

D

E

C

T

F

F

S

S

H

H

A

R

K

R

S

S

I

C

L

L

S

V

K

R

S

P

T

L

W

M

I

N

E

D

A

E

N

G

L

L

K

K

active site: N114 (= N130), K137 (= K153), E209 (= E225), C243 (= C259), E333 (= E347), Y412 (= Y426)
binding 1-phenylpropan-1-one: Y115 (≠ F131), L119 (≠ I135), C243 (= C259), I394 (≠ F408)

4l2oA Crystal structure of human aldh3a1 with its selective inhibitor 1-(4- fluorophenyl)sulfonyl-2-methylbenzimidazole
43% identity, 93% coverage: 17:453/469 of query aligns to 1:439/446 of 4l2oA

query
sites
4l2oA

T

S

E

K

V

I

K

S

A

E

L

A

I

V

D

K

T

R

Q

A

R

R

K

A

F

A

F

F

Y

S

S

S

N

G

A

R

T

T

K

R

P

P

V

L

S

Q

F

F

R

R

I

I

A

Q

Q

Q

L

L

K

E

K

A

L

L

K

Q

Q

R

L

L

L

I

K

Q

D

E

N

Q

E

E

A

Q

Q

E

L

L

H

V

K

G

A

A

I

L

Y

A

A

A

D

D

L

L

R

H

K

K

S

N

E

E

F

W

D

N

N

A

L

Y

T

Y

T
x
E

E

E

L

V

P
x
Y

L

V

Y

L

S

E

E

E

I

I

D

E

Y

Y

A

M

V

I

K

Q

N

K

L

L

K

P

K

E

W

W

A

A

T

A

P

D

K

E

R

P

V

V

K

E

T

K

T

T

I

P

L

Q

N

T

F

Q

P

Q

S

D

K

E

S

L

Y

Y

I

I

V

H

A

S

E

E

P

P

L

L

G

G

V

V

S

V

L

L

I

V

I

I

G

G

A
x
T

W
|
W

N
|
N

F
x
Y

P

P

Y

F

N
|
N

I
x
L

A

T

L

I

I

Q

P

P

I

M

V

V

G

G

A

A

I

I

A

A

G

G

N

N

T

A

S

V

I

V

L

L

K
|
K

P

P

S

S

E
|
E

M

L

A

S

P

E

A

N

T

M

S

A

A

S

I

L

M

L

A

A

E

T

L

I

I

I

N

P

K

Q

N

Y

F

L

P

D

S

K

N

D

Y

L

L

Y

K

P

V

V

L

I

Q

N

G

G

I
x
V

P

P

E

E

T

T

A

E

I

L

L

L

K

K

Q

E

R

R

F

F

D

D

K

H

I

I

F

L

F

Y

T
|
T

G
|
G

S
|
S

P

T

Q

G

V
|
V

G

G

K

K

I

I

V

I

N

M

Q

T

A

A

P

K

H

H

L

L

T

T

N

P

V

V

T

T

L

L

E
|
E

L
|
L

G
|
G

G

G

K

K

N

S

P

P

A

C

I

Y

F

V

T

D

K

K

D

N

C

C

S

D

L

L

N

D

I

V

G

A

V

C

K

R

R

R

M

I

V

A

S

W

G

G

K

K

F

F

L
x
M

N

N

A

S

G

G

Q

Q

I

T

C
|
C

I

V

T

A

S

P

D

D

Y

Y

V

I

L

L

V

C

Q

D

K

P

D

S

I

I

K

Q

E

N

K

Q

F

I

L

V

T

E

Q

K

V

L

V

K

A

K

E

S

I

L

K

K

K

E

-

F

-

Y

Q

G

N

E

Y

D

S

A

I

K

E

K

N

S

G

R

N

D

F

Y

V

G

E

R

L

I

I

I

N

S

D

A

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R

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x
H

F

F

D

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R

I

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M

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G

L

L

I

I

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E

S

G

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Q

K

K

V

V

Y

A

Y

Y

G

G

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T

Y

G

I

D

K

A

E

A

T

T

R

R

F

Y

I

I

E

A

P

P

T

T

I

I

L

L

T

T

N

D

V

V

S

D

V

P

D

Q

D

S

A

P

V

V

M

M

Q

Q

E

E

E
|
E

I

I

F
|
F

G

G

P

P

V

V

L

L

P

P

V

I

I

V

E

C

Y

V

D

R

T

S

I

L

D

E

E

E

A

A

F

I

A

Q

I

F

I

I

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K

Q

F

R

E

E

K

K

P

P

L

L

S

A

A

L

Y

Y

L

M

F

F

S

S

N

S

N

N

S

D

T

K

I

V

K

I

K

K

R

K

F

M

L

I

N

A

E

E

V

T

S

S

F

S

G

G

N

G

G

V

A

A

I

A

N

N

D

D

S

V

I
|
I

M

V

Q

H

F
x
I

T
|
T

N

L

H

H

N

S

L

L

P

P

F
|
F

G

G

V

V

G

G

N

N

S

S

G

G

T

M

G

G

S

S

Y
|
Y

H
|
H

G

G

K

K

F

K

S

S

F

F

D

E

C

T

F

F

S

S

H

H

A

R

K

R

S

S

I

C

L

L

S

V

K

R

S

P

T

L

W

M

I

N

E

D

A

E

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G

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L

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K

active site: N114 (= N130), K137 (= K153), E209 (= E225), C243 (= C259), E333 (= E347), Y412 (= Y426)
binding 1-[(4-fluorophenyl)sulfonyl]-2-methyl-1H-benzimidazole: E61 (≠ T77), Y65 (≠ P81), Y115 (≠ F131), N118 (= N134), L119 (≠ I135), M237 (≠ L253), C243 (= C259), I391 (= I405), I394 (≠ F408), T395 (= T409), F401 (= F415), H413 (= H427)
binding nicotinamide-adenine-dinucleotide: T112 (≠ A128), W113 (= W129), N114 (= N130), L119 (≠ I135), E140 (= E156), V169 (≠ I185), T186 (= T202), G187 (= G203), S188 (= S204), V191 (= V207), E209 (= E225), L210 (= L226), G211 (= G227), C243 (= C259), H289 (≠ N303), E333 (= E347), F335 (= F349), F401 (= F415)

4h80A Crystal structure of human aldh3a1 with its isozyme selective inhibitor - n-[4-(4-methylsulfonyl-2-nitroanilino)phenyl]acetamide
43% identity, 93% coverage: 17:453/469 of query aligns to 1:439/446 of 4h80A

query
sites
4h80A

T

S

E

K

V

I

K

S

A

E

L

A

I

V

D

K

T

R

Q

A

R

R

K

A

F

A

F

F

Y

S

S

S

N

G

A

R

T

T

K

R

P

P

V

L

S

Q

F

F

R

R

I

I

A

Q

Q

Q

L

L

K

E

K

A

L

L

K

Q

Q

R

L

L

L

I

K

Q

D

E

N

Q

E

E

A

Q

Q

E

L

L

H

V

K

G

A

A

I

L

Y

A

A

A

D

D

L

L

R

H

K

K

S

N

E

E

F

W

D

N

N

A

L

Y

T

Y

T
x
E

E

E

L

V

P
x
Y

L

V

Y

L

S

E

E

E

I

I

D

E

Y

Y

A

M

V

I

K

Q

N

K

L

L

K

P

K

E

W

W

A

A

T

A

P

D

K

E

R

P

V

V

K

E

T

K

T

T

I

P

L

Q

N

T

F

Q

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Q

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D

K

E

S

L

Y

Y

I

I

V

H

A

S

E

E

P

P

L

L

G

G

V

V

S

V

L

L

I

V

I

I

G

G

A

T

W

W

N
|
N

F
x
Y

P

P

Y

F

N
|
N

I

L

A

T

L

I

I

Q

P

P

I

M

V

V

G

G

A

A

I

I

A

A

G

G

N

N

T

A

S

V

I

V

L

L

K
|
K

P

P

S

S

E

E

M

L

A

S

P

E

A

N

T

M

S

A

A

S

I

L

M

L

A

A

E

T

L

I

I

I

N

P

K

Q

N

Y

F

L

P

D

S

K

N

D

Y

L

L

Y

K

P

V

V

L

I

Q

N

G

G

I

V

P

P

E

E

T

T

A

E

I

L

L

L

K

K

Q

E

R

R

F

F

D

D

K

H

I

I

F

L

F

Y

T

T

G

G

S

S

P

T

Q

G

V

V

G

G

K

K

I

I

V

I

N

M

Q

T

A

A

P

K

H

H

L

L

T

T

N

P

V

V

T

T

L

L

E
|
E

L

L

G

G

K

K

N

S

P

P

A

C

I

Y

F

V

T

D

K

K

D

N

C

C

S

D

L

L

N

D

I

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G

A

V

C

K

R

R

R

M

I

V

A

S
x
W

G

G

K

K

F

F

L

M

N

N

A

S

G

G

Q

Q

I
x
T

C
|
C

I
x
V

T

A

S

P

D

D

Y

Y

V

I

L

L

V

C

Q

D

K

P

D

S

I

I

K

Q

E

N

K

Q

F

I

L

V

T

E

Q

K

V

L

V

K

A

K

E

S

I

L

K

K

K

E

-

F

-

Y

Q

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Y

D

S

A

I

K

E

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N

S

G

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N

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F

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V

M

S

G

L

L

I

I

D

E

S

G

Q

Q

K

K

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V

Y

A

Y

Y

G

G

N

T

Y

G

I

D

K

A

E

A

T

T

R

R

F

Y

I

I

E

A

P

P

T

T

I

I

L

L

T

T

N

D

V

V

S

D

V

P

D

Q

D

S

A

P

V

V

M

M

Q

Q

E

E

E
|
E

I

I

F

F

G

G

P

P

V

V

L

L

P

P

V

I

I

V

E

C

Y

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D

R

T

S

I

L

D

E

E

E

A

A

F

I

A

Q

I

F

I

I

Q

N

K

Q

F

R

E

E

K

K

P

P

L

L

S

A

A

L

Y

Y

L

M

F

F

S

S

N

S

N

N

S

D

T

K

I

V

K

I

K

K

R

K

F

M

L

I

N

A

E

E

V

T

S

S

F

S

G

G

N

G

G

V

A

A

I

A

N

N

D

D

S

V

I

I

M

V

Q

H

F
x
I

T
|
T

N

L

H

H

N

S

L

L

P

P

F
|
F

G

G

V

V

G

G

N

N

S

S

G

G

T

M

G

G

S

S

Y
|
Y

H

H

G

G

K

K

F

K

S

S

F

F

D

E

C

T

F

F

S

S

H

H

A

R

K

R

S

S

I

C

L

L

S

V

K

R

S

P

T

L

W

M

I

N

E

D

A

E

N

G

L

L

K

K

active site: N114 (= N130), K137 (= K153), E209 (= E225), C243 (= C259), E333 (= E347), Y412 (= Y426)
binding N-(4-{[4-(methylsulfonyl)-2-nitrophenyl]amino}phenyl)acetamide: E61 (≠ T77), Y65 (≠ P81), Y115 (≠ F131), N118 (= N134), W233 (≠ S249), T242 (≠ I258), C243 (= C259), V244 (≠ I260), I394 (≠ F408), T395 (= T409), F401 (= F415)

Q70DU8 Aldehyde dehydrogenase family 3 member H1; AtALDH4; Ath-ALDH4; EC 1.2.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
40% identity, 96% coverage: 17:468/469 of query aligns to 10:468/484 of Q70DU8

query
sites
Q70DU8

T

A

E

E

V

A

K

S

A

N

L

L

I

V

D

T

T

E

Q

L

R

R

K

R

F

S

F

F

Y

D

S

D

N

G

A

V

T

T

K

R

P

G

V

Y

S

E

F

W

R

R

I

V

A

T

Q

Q

L

L

K

K

L

L

K

M

Q

I

L

I

L
x
C

K

D

D

N

N

H

E

E

A

P

Q

E

L

I

H

V

K

A

A

A

I

L

Y

R

A

D

D

D

L

L

R

G

K

K

S

P

E

E

F

L

D

E

N

S

L

S

T

V

T

Y

E

E

L

V

L

S

P

L

L

L

Y

R

S

N

E

S

I

I

D

K

Y

L

A

A

V

L

K

K

N

Q

L

L

K

K

K

N

W

W

A

M

T

A

P

P

K

E

R

K

V

A

K

K

T

T

T

S

I

L

L

T

N

T

F

F

P

P

S

A

K

S

S

A

Y

E

I

I

V

V

A

S

E

E

P

P

L

L

G

G

V

V

S

V

L

L

I

V

I

I

G

S

A

A

W

W

N

N

F

Y

P

P

Y

F

N

L

I

L

A

S

L

I

I

D

P

P

I

V

V

I

G

G

A

A

I

I

A

S

A

A

G

G

N

N

T

A

S

V

I

V

L

L

K

K

P

P

S

S

E
|
E

M

L

A

A

P

P

A

A

T

S

S

S

A

A

I

L

M

L

A

T

E

K

L

L

I

L

N

E

K

Q

N

Y

F

L

P

D

S

P

N

S

Y

A

L

V

K

R

V

V

L

V

Q

E

G

G

G

A

I
x
V

P

T

E

E

T

T

T

S

A

A

I

L

L

L

K

E

Q

Q

R

K

F

W

D

D

K

K

I

I

F

F

F

Y

T

T

G

G

S

S

P

S

Q

K

V
x
I

G

G

K

R

I

V

V

I

N

M

Q

A

A

A

P

K

H

H

L

L

T

T

N

P

V

V

T

V

L

L

E

E

L

L

G

G

K

K

N

S

P

P

A

V

I

V

F

V

T

D

K

S

D

D

C

T

S

D

L

L

N

K

I

V

G

T

V

V

K

R

R

R

M

I

V

I

S

V

G

G

K

K

F

W

-

G

L
x
C

N

N

A

N

G

G

Q

Q

I

A

C
|
C

I

V

T

S

S

P

D

D

Y

Y

V

I

L

L

V

T

Q

T

K

K

D

E

I

Y

K

A

E

P

K

K

F

L

L

I

T

D

Q

A

V

M

V

K

A

L

E

E

I

L

K

E

K

K

-

F

-

Y

Q

G

N

K

Y

N

S

P

I

I

E

E

N

S

G

K

N

D

F

M

V

S

E

R

L

I

I

V

N

N

D

S

K

N

N

H

F

F

D

D

R

R

I

L

V

S

S

K

L

L

I

L

D

D

-

E

-

K

-

E

-

V

S

S

Q

D

K

K

V

I

Y

V

Y

Y

G

G

N

E

Y

K

I

D

K

R

E

E

T

N

R

L

F

K

I

I

E

A

P

P

T

T

I

I

L

L

T

L

N

D

V

V

S

P

V

L

D

D

D

S

A

L

V

I

M

M

Q

S

E

E

I

I

F

F

G

G

P

P

V

L

L

L

P

P

V

I

I

L

E

T

Y

L

D

N

T

N

I

L

D

E

E

E

A

S

F

F

A

D

I

V

I

I

Q

R

K

S

F

R

E

P

K

K

P

P

L

L

S

A

A

A

Y

Y

L

L

F

F

S

T

N

H

N

N

S

K

T

K

I

L

K

K

K

E

R

R

F

F

L

A

N

A

E

T

V

V

S

S

F

A

G

G

N

G

G

I

A

V

I

V

N

N

D

D

S

I

I

A

M

V

Q

H

F

L

T

A

N

L

H

H

N

T

L

L

P

P

F

F

G

G

V

V

G

G

N

E

S

S

G

G

T

M

G

G

S

A

Y

Y

H

H

G

G

K

K

F

F

S

S

F

F

D

D

C

A

F

F

S

S

H

H

A

K

K

K

S

A

I

V

L

L

S

Y

K

R

S

S

T

L

W

F

I

G

E

D

A

S

N

A

L

V

K

R

Y

Y

Y

P

E

P

H

Y

T

S

D

R

T

G

K

K

M

L

K

R

I

L

K

K

K

A

L

L

C45 (≠ L52) mutation to S: Decreased solubility, loss of dimerization and strongly decreased activity.
E149 (= E156) mutation to D: Small effect on NAD(+) interaction, but 40% loss of efficiency. Ability to use NADP(+). 70% loss of efficiency with NAD(+); when associated with V-200. Impaired affinity for both cofactors and decreased catalytic efficiency; when associated with G-200.; mutation to N: Ability to use NADP(+) and 33% decreased efficiency with NAD(+). 70% loss of efficiency with NAD(+); when associated with V-200. Impaired affinity for both cofactors and decreased catalytic efficiency; when associated with G-200.; mutation to Q: Loss of specificity for NAD(+) and loss of 25% efficiency. 15% efficiency with NAD(+); when associated with V-200. Impaired affinity for both cofactors and decreased catalytic efficiency; when associated with G-200.; mutation to T: Loss of specificity and increased NADP(+) binding. Decreased catalytic efficiency. Loss of cofactor specificity and same lower efficiency with both; when associated with V-200. Impaired affinity for both cofactors and decreased catalytic efficiency; when associated with G-200. Changed coenzyme preference from NAD(+) to NADP(+), but no effect on the catalytic efficiency; when associated with R-178 and V-200.
V178 (≠ I185) mutation to R: Changed coenzyme preference from NAD(+) to NADP(+), but no effect on the catalytic efficiency; when associated with T-149 and V-200.
I200 (≠ V207) mutation to G: Changed coenzyme preference from NAD(+) to NADP(+), but impaired affinities for both cofactors. No effect on the interaction with the substrate. Impaired affinities for both cofactors and decreased catalytic efficiencies; when associated with D-149, Q-149, N-149 or T-149.; mutation to V: Also able to use NADP(+) as coenzyme, but no effect on the interaction with the substrate. 15% efficiency with NAD(+); when associated with Q-149. 70% loss of efficiency with NAD(+); when associated with D-149 or N-149. Loss of cofactor specificity and same lower efficiency with both; when associated with T-149. Changed coenzyme preference from NAD(+) to NADP(+), but no effect on the catalytic efficiency; when associated with T-149 and R-178.
C247 (≠ L253) mutation to S: No effect on solubility, but 10% loss of activity.
C253 (= C259) mutation to S: No effect on solubility, but loss of activity.

1ad3A Class 3 aldehyde dehydrogenase complex with nicotinamide-adenine- dinucleotide (see paper)
45% identity, 89% coverage: 27:445/469 of query aligns to 10:430/446 of 1ad3A

query
sites
1ad3A

R

R

K

E

F

A

F

F

Y

N

S

S

N

G

A

K

T

T

K

R

P

S

V

L

S

Q

F

F

R

R

I

I

A

Q

Q

Q

L

L

K

E

K

A

L

L

K

Q

Q

R

L

M

L

I

K

N

D

E

N

N

E

L

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G

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G

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A

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W

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Y

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P

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N

I

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A

T

L

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I

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P

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A

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L

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N

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M

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|
Y

H

H

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G

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K

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K

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active site: N113 (= N130), K136 (= K153), E208 (= E225), C242 (= C259), E332 (= E347), Y411 (= Y426)
binding nicotinamide-adenine-dinucleotide: A111 (= A128), W112 (= W129), N113 (= N130), E139 (= E156), V140 (≠ M157), V168 (≠ I185), G186 (= G203), V190 (= V207), H288 (≠ N303), R291 (= R306), E332 (= E347), F334 (= F349)

Q8W033 Aldehyde dehydrogenase family 3 member I1, chloroplastic; AtALDH3; Ath-ALDH3; EC 1.2.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
42% identity, 96% coverage: 18:469/469 of query aligns to 74:532/550 of Q8W033

query
sites
Q8W033

E

E

V

A

K

A

A

L

L

L

I

V

D

D

T

E

Q

L

R

R

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I

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N

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A

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M

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D

D

E

N

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E

A

A

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Y

A

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D

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N

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N

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L

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P

L

L

G

G

V

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V

L

L

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A

A

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W

N

N

F

F

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P

Y

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N

L

I

L

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L

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I

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P

P

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V

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G

G

A

A

I

I

A

A

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G

N

N

T

A

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L

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A

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A

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A

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D

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F

A

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G

N

G

G

I

A

T

I

I

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N

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T

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L

Q

H

F

V

T

T

N

V

H

K

N

D

L

L

P

P

F

F

G

G

V

V

G

G

N

E

S

S

G

G

T

I

G

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A

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Y

H

H

G

G

K

K

F

F

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S

F

Y

D

E

C

T

F

F

S

S

H

H

A

K

K

K

S

G

I

V

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F

W

S

I

G

E

D

A

A

N

D

L

L

K

R

Y

Y

Y

P

E

P

H

Y

T

T

D

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L

C114 (≠ Q58) mutation to S: No effect on solubility, but loss of dimerization and 80% loss of activity.
C142 (≠ I86) mutation to S: No effect on solubility, but decreased activity.
V263 (= V207) mutation to I: No effect on substrate specificity, but decreased affinity for NADP(+) and increased affinity for NAD(+).
C286 (≠ N230) mutation to S: No effect on solubility, but no effect on activity.
C310 (≠ L253) mutation to S: No effect on solubility, but no effect on activity.
C316 (= C259) mutation to S: No effect on solubility, but loss of activity.

P43353 Aldehyde dehydrogenase family 3 member B1; Aldehyde dehydrogenase 7; Long-chain fatty aldehyde dehydrogenase; Medium-chain fatty aldehyde dehydrogenase; EC 1.2.1.28; EC 1.2.1.5; EC 1.2.1.7; EC 1.2.1.48 from Homo sapiens (Human) (see paper)
41% identity, 95% coverage: 22:465/469 of query aligns to 4:454/468 of P43353

query
sites
P43353

L

L

I

G

D

D

T

T

Q

L

R

R

K

R

F

L

-

R

-

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-

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F

F

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I

A

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Q

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G

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F

L

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N

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A

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I

L

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Y

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S

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Y

L

A

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N

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W

A

M

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D

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R

R

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T

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I

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L

A

N

T

F

Q

P

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S

D

K

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S

A

Y

F

I

I

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R

A

K

E

E

P

P

L

F

G

G

V

L

S

V

L

L

I

I

G

A

A

P

W

W

N

N

F

Y

P

P

Y

L

N

N

I

L

A

T

L

L

I

V

P

P

I

L

V

V

G

G

A

A

I

L

A

A

G

G

N

N

T

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V

I

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L

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A

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A

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A

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L

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Y

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G

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T

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L

L

L

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F

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G

G

S

S

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P

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G

G

K

K

I

I

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V

N

M

Q

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A

A

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H

H

L

L

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T

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P

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V

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T

L

L

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E

L

L

G

G

K

K

N

N

P

P

A

C

I

Y

F

V

T

D

K

D

D

N

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C

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D

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N

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A

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K

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P

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N

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N

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R

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N

T

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C

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G

N

N

D

D

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M

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T

N

L

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A

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F

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L

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N

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L

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S

D

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T

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K

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K

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L

Sites not aligning to the query:

463 modified: S-palmitoyl cysteine
465 modified: S-geranylgeranyl cysteine

E9Q3E1 Aldehyde dehydrogenase family 3 member B2; Aldh3B2; Aldehyde dehydrogenase 8; Long-chain fatty aldehyde dehydrogenase; EC 1.2.1.3; EC 1.2.1.48 from Mus musculus (Mouse) (see paper)
40% identity, 98% coverage: 7:465/469 of query aligns to 5:467/479 of E9Q3E1

query
sites
E9Q3E1

K

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D

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T

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E

E

I

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A

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A

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N

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A

A

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A

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S

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A

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F

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G

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L

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I

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A

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W

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N

F

Y

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P

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V

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N

I

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A

M

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I

I

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G

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A

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A

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A

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N

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W462 (≠ T460) mutation to A: Reduces lipid droplet localization.

Sites not aligning to the query:

469 W→A: Reduces lipid droplet localization.
476 C→S: Reduces lipid droplet localization.

J3QMK6 Aldehyde dehydrogenase family 3 member B3; EC 1.2.1.3 from Mus musculus (Mouse) (see paper)
39% identity, 98% coverage: 7:466/469 of query aligns to 9:468/479 of J3QMK6

query
sites
J3QMK6

K

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F

G

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L

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I

G

A

A

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N

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G

A

A

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A

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N

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x
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RR 462:463 (≠ TK 460:461) mutation to AA: Reduces membrane localization.

Q80VQ0 Aldehyde dehydrogenase family 3 member B1; Aldehyde dehydrogenase 7; Long-chain fatty aldehyde dehydrogenase; Medium-chain fatty aldehyde dehydrogenase; EC 1.2.1.28; EC 1.2.1.5; EC 1.2.1.7; EC 1.2.1.48 from Mus musculus (Mouse) (see paper)
39% identity, 97% coverage: 12:465/469 of query aligns to 1:454/468 of Q80VQ0

query
sites
Q80VQ0

I

M

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A

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D

N

N

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A

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D

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L

A

A

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N

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W

A

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A

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L

F

G

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L

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I

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A

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W

N

N

F

Y

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P

Y

I

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N

I

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A

T

L

L

I

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P

P

I

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G

G

A

A

I

I

A

A

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G

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A

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E

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H

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K

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F

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D

K

Y

I

I

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F

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T

G

G

S

N

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A

A

A

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H

H

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L

T

T

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P

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I

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T

L

L

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E

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G

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K

N

N

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K

D

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A

K

N

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G

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F

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N

A

A

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G

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S

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D

D

Y

Y

V

I

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L

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K

Q

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A

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A

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A

I

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K

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K

R

-

F

-

Y

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N

G

G

F

A

C

I

G

N

N

D

D

S

G

I

F

M

M

Q

H

F

M

T

T

N

L

H

S

N

S

L

L

P

P

F

F

G

G

V

V

G

G

N

T

S

S

G

G

T

M

G

G

S

R

Y

Y

H

H

G

G

K

K

F

F

S

S

F

F

D

D

C

T

F

F

S

S

H

N

A

Q

K

R

S

A

I

C

L

L

S

L

K

R

S

S

T

P

W

G

I

M

E

E

A

K

-

I

-

N

N

D

L

L

K

R

Y

Y

Y

P

E

P

H

Y

T

S

D

S

T

R

K

N

M

L

K

R

I

V

I

L

Sites not aligning to the query:

462 modified: S-palmitoyl cysteine; C→S: Reduces palmitoylation.
462:463 CC→SS: Abolishes palmitoylation.
463 modified: S-palmitoyl cysteine; C→S: Reduces palmitoylation.

5ucdA Benzaldehyde dehydrogenase, a class 3 aldehyde dehydrogenase, with bound NADP+ and benzoate adduct (see paper)
39% identity, 91% coverage: 17:441/469 of query aligns to 6:431/435 of 5ucdA

query
sites
5ucdA

T

A

E

K

V

I

K

D

A

S

L

L

I

F

D

S

T

A

Q

Q

R

K

K

A

F

Y

F

F

Y

A

S

T

N

R

A

A

T

T

K

A

P

D

V

V

S

G

F

F

R

R

I

K

A

Q

Q

S

L

L

K

E

K

R

L

L

K

K

Q

E

L

A

L

V

K

I

D

N

N

N

E

K

A

E

Q

A

L

L

H

Y

K

S

A

A

I

L

Y

A

A

E

D

D

L

L

R

G

K

K

S

P

E

K

F

D

D

V

N

V

L

D

T

L

T

A

E

E

L

I

L

G

P

A

L

V

Y

L

S

H

E

E

I

I

D

D

Y

F

A

A

V

L

K

A

N

H

L

L

K

D

K

E

W

W

A

V

T

A

P

P

K

V

R

S

V

V

K

P

T

S

T

P

I

D

L

I

N

I

F

A

P

P

S

S

K

E

S

C

Y

Y

I

V

V

V

A

Q

E

E

P

P

L

Y

G

G

V

V

S

T

L

Y

I

I

I
|
I

G
|
G

A

P

W
x
F

N
|
N

F

Y

P

P

Y

V

N

N

I

L

A

T

L

L

I

T

P

P

I

L

V

I

G

G

A

A

I

I

A

I

A

G

G

G

N

N

T

T

S

C

I

I

L

I

K
|
K

P

P

S
|
S

E
|
E

M

T

A

T

P

P

A

E

T

T

S

S

A

A

I

V

M

I

A

E

E

K

L

I

I

I

N

A

K

E

N

A

F

F

P

A

S

P

N

E

Y

Y

L

V

K

A

V

V

L

I

Q

Q

G

G

I
x
R

P

D

E

E

T

N

T

S

A

H

I

L

L

L

K

S

Q

L

R

P

F

F

D

D

K

F

I

I

F

F

F
|
F

T
|
T

G
|
G

S
|
S

P

P

Q

N

V
|
V

G

G

K

K

I

V

V

V

N

M

Q

Q

A

A

P

K

H

H

L

L

T

T

N

P

V

V

T

V

L

L

E
|
E

L
|
L

G

G

K

K

N

C

P

P

A

L

I

I

F

V

T

L

K

P

D

D

C

A

S

D

L

L

N

D

I

Q

G

T

V

V

K

N

R

Q

M

L

V

M

S

F

G

G

K

K

F

F

L

I

N

N

A

S

G

G

Q

Q

I

T

C
|
C

I

I

T

A

S

P

D

D

Y

Y

V

L

L

Y

V

V

Q

H

K

Y

D

S

I

V

K

K

E

D

K

A

F

L

L

L

T

E

Q

R

V

L

V

V

A

E

E

R

I

V

K

K

K

T

Q

E

N

L

Y

P

S

E

I

I

E

N

N

S

G

T

N

G

F

-

V

-

E

K

L

L

I

V

N

T

D

E

K

R

N

Q

F

V

D

Q

R

R

I

L

V

V

S

S

L

L

I

L

D

E

S

A

Q

T

-

Q

-

G

K

Q

V

V

Y

L

Y

V

G

G

G

S

N

Q

Y

A

I

D

K

V

E

S

T

K

R

R

F

A

I

L

E

S

P

A

T

T

I

V

L

V

T

D

N

G

V

V

S

E

V

W

D

N

D

D

A

P

V

L

M

M

Q

S

E

E

E
|
E

I

L

F
|
F

G

G

P

P

V

I

L

L

P

P

V

V

I

L

E

E

Y

F

D

D

T

S

I

V

D

R

E

T

A

A

F

I

A

D

I

Q

I

V

Q

N

K

K

F

H

E

H

-

P

K

K

P

P

L

L

S

A

A

V

Y

Y

L

V

F

F

S

G

N

K

N

D

S

M

T

D

I

V

K

A

K

K

R

G

F

I

L

I

N

N

E

Q

V

I

S

Q

F

S

G

G

N

D

G

A

A

Q

I

V

N

N

D

G

S

V

I

M

M

L

Q

H

F

A

T

F

N

S

H

P

N

Y

L

L

P

P

F

F

G

G

V

I

G

G

N

A

S

S

G

G

T

M

G

G

S

E

Y
|
Y

H

H

G

G

K

H

F

F

S

S

F

Y

D

L

C

T

F

F

S

T

H

H

A

K

K

K

S

S

I

V

active site: N119 (= N130), K142 (= K153), E214 (= E225), C248 (= C259), E336 (= E347), Y416 (= Y426)
binding nadp nicotinamide-adenine-dinucleotide phosphate: I115 (= I126), G116 (= G127), F118 (≠ W129), N119 (= N130), K142 (= K153), S144 (= S155), E145 (= E156), R174 (≠ I185), F190 (= F201), T191 (= T202), G192 (= G203), S193 (= S204), V196 (= V207), E214 (= E225), L215 (= L226), C248 (= C259), E336 (= E347), F338 (= F349)

5nnoA Structure of tbaldh3 complexed with NAD and an3057 aldehyde (see paper)
36% identity, 98% coverage: 11:469/469 of query aligns to 5:484/484 of 5nnoA

query
sites
5nnoA

E

E

I

N

T

T

S

S

F

L

E

E

T

N

E

-

V

I

K

P

A

V

L

I

I

V

D

S

T

K

Q

C

R

R

K

E

F

A

F

F

Y

N

S

D

N

D

A

A

T

N

K

R

P

D

V

L

S

K

F

K

R

R

I

K

A

Q

Q

V

L

L

K

R

K

S

L

L

K

L

Q

N

L

L

L

V

K

E

D

E

N

N

E

T

A

D

Q

E

L

F

H

C

K

K

A

A

I

I

Y

H

A

R

D

D

L

R

R

R

K

R

S

H

E

R

F

D

D

E

N

T

L

V

T

V

T

M

E

E

L

I

L

L

P
|
P

L

L

Y

R

S

N

E

E

I

V

D

W

Y

H

A

L

V

I

K

E

N

H

L

M

K

D

K

E

W

Y

A

V

T

K

P

P

K

-

R

-

V

V

K

K

T

P

T

T

I

M

L

E

N

G

F

A

P

A

S

A

-

L

-

D

K

D

S

C

Y

E

I

L

V

Q

A

Y

E

E

P

P

L

L

G

G

V

V

S

V

L

L

I

V

I
|
I

G
|
G

A

T

W
|
W

N
|
N

F
x
Y

P

P

Y

L

N
x
L

I
x
L

A

I

L

L

I

Q

P

P

I

L

V

L

G

G

A

A

I

L

A

A

G

G

N

N

T

T

S

A

I

V

L

I

K
|
K

P

P

S

S

E
|
E

M

L

A

A

P

P

A

A

T

T

S

A

A

E

I

L

M

L

A

T

E

K

L

L

I

L

N

P

K

K

N

Y

F

V

P

S

S

S

N

D

Y

V

L

V

K

G

V

I

L

V

Q

N

G

G

I
x
V

P

S

E

E

T
|
T

T

T

A

A

I

V

L

L

K

K

Q

E

R

R

F

F

D

D

K

H

I

I

F

L

F
x
Y

T
|
T

G
|
G

S
|
S

P

A

Q

R

V
|
V

G

A

K

E

I

I

V

V

N

M

Q

A

A

A

P

K

H

H

L

L

T

T

N

P

V

V

T

T

L

L

E
|
E

L
|
L

G

G

K

K

N

S

P

P

A

V

I

V

F

V

T

D

K

D

D

T

C

C

S

A

L

D

N

N

I

M

G

K

V

V

-

V

-

A

K

E

R

R

M

I

V

M

S

W

G

G

K

K

F

I

L

I

N

N

A

A

G

G

Q

Q

I
x
T

C
x
S

I

I

T

A

S

P

D

D

Y

Y

V

V

L

V

V

V

Q

E

K

K

D

S

I

M

K

E

-

S

-

V

-

L

-

V

-

D

-

A

-

L

-

A

-

E

-

A

-

R

-

K

-

A

-

M

-

L

-

G

E

D

K

K

F

F

L

L

T

K

Q

V

V

L

V

K

A

G

E

E

-

L

I

V

K

K

K

Q

Q

K

N

Q

Y

Q

S

F

I

L

E

E

N

E

G

S

N

D

F

Y

V

P

E

R

L

I

I

V

N

N

D

A

K

S

N

H

F

F

D

Q

R

R

I

L

V

M

S

E

L

F

I

M

D

K

S

G

Q

G

K

K

V

V

Y

A

Y

V

G

G

N

E

Y

A

I

D

K

E

E

A

T

T

R

L

F

T

I

I

E

A

P

P

T

T

I

I

L

L

T

T

N

N

V

I

S

D

V

P

D

T

D

H

A

P

V

V

M

M

Q

Q

E

E

E
|
E

I

I

F
|
F

G

G

P

P

V

I

L

L

P

P

V

V

I

L

E

T

Y

Y

D

E

T

N

I

E

D

K

E

D

A

I

F

L

A

K

I

I

Q

N

K

S

F

R

E

E

K

K

P

P

L

L

S

A

A

L

Y

Y

L

V

F

F

S

S

N

N

S

K

T

R

I

F

K

I

K

R

R

G

F

V

L

E

N

S

E

R

V

T

S

S

F

S

G

G

N

A

G

V

A

V

I

V

N

N

D

D

S

V

I

V

M

V

Q

H

F

A

T
x
G

N

A

H

D

N

G

L

L

P

P

F
|
F

G

G

V

V

G

G

N

R

S

S

G

G

T

M

G

G

S

A

Y
|
Y

H

H

G

G

K

R

F

Y

S

S

F

F

D

E

C

T

F

F

S

S

H

H

A

R

K

R

S

P

I

V

L

M

S

R

K

R

S

G

T

F

W

L

I

F

E

S

A

S

-

I

-

D

N

T

L

V

K

R

Y

F

Y

P

E

P

H

Y

T

T

D

T

T

A

K

K

M

S

K

R

I

V

I

L

K

N

K

S

L

L

F

L

active site: N123 (= N130), K146 (= K153), E218 (= E225), S254 (≠ C259), E360 (= E347), Y439 (= Y426)
binding 4-[(1-oxidanyl-3~{H}-2,1-benzoxaborol-5-yl)oxy]benzaldehyde: P74 (= P81), Y124 (≠ F131), L127 (≠ N134), T253 (≠ I258), S254 (≠ C259), G422 (≠ T409)
binding nicotinamide-adenine-dinucleotide: I119 (= I126), G120 (= G127), W122 (= W129), N123 (= N130), L128 (≠ I135), K146 (= K153), E149 (= E156), V178 (≠ I185), T181 (= T188), Y194 (≠ F201), T195 (= T202), G196 (= G203), S197 (= S204), V200 (= V207), E218 (= E225), L219 (= L226), S254 (≠ C259), E360 (= E347), F362 (= F349), F428 (= F415)

Q04458 Fatty aldehyde dehydrogenase HFD1; Hexadecenal dehydrogenase; EC 1.2.1.3; EC 1.2.1.64 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 2 papers)
34% identity, 96% coverage: 17:466/469 of query aligns to 15:491/532 of Q04458

query
sites
Q04458

T

S

E

K

V

I

K

D

A

E

L

I

I

V

D

E

T

I

Q

S

R

R

K

N

F

F

Y

F

S

E

N

K

A

Q

-

L

-

K

-

L

-

S

-

H

-

E

-

N

-

P

-

R

T

K

K

K

P

D

V

L

S

E

F

F

R

R

I

Q

A

L

Q

Q

L

L

K

K

L

L

K

Y

Q

Y

L

A

L

V

K

K

D

D

N

H

E

E

A

E

Q

E

L

L

H

I

K

D

A

A

I

M

Y

Y

A

K

D

D

L

F

R

H

K

R

S

N

E

K

F

I

D

E

N

S

L

V

T

L

T

N

E

E

L

T

P

K

L

L

Y

M

S

N

E

D

I

I

D

L

Y

H

A

L

V

I

K

E

N

I

L

L

K

P

K

K

W

L

A

I

T

K

P

P

K

R

R

R

V

V

K

S

T

D

T

S

I

S

L

P

N

P

F

F

P

M

S

F

K

G

S

K

Y

T

I

I

V

V

A

E

E

K

-

I

P

S

L

R

G

G

V

S

S

V

L

L

I

I

G

A

A

P

W

F

N

N

F

F

P

P

Y

L

N

L

I

L

A

A

L

F

I

A

P

P

I

L

V

A

G

A

A

A

I

L

A

A

G

G

N

N

T

T

S

I

I

V

L

L

K

K

P

P

S

S

E

E

M

L

A

T

P

P

A

H

T

T

S

A

A

V

I

V

M

M

A

E

E

N

L

L

I

L

N

T

-

T

K

A

N

G

F

F

P

P

S

D

N

G

Y

L

L

I

K

Q

V

V

L

V

Q

Q

G

G

G

A

I

I

P

D

E

E

T

T

A

R

I

L

L

L

K

D

-

C

Q

G

R

K

F

F

D

D

K

L

I

I

F

F

F

Y

T

T

G

G

S

S

P

P

Q

R

V

V

G

G

K

S

I

I

V

V

N

A

Q

E

A

K

A

A

P

K

H

S

L

L

T

T

N

P

V

C

T

V

L

L

E

E

L

L

G

G

K

K

N
x
S

P

P

A

T

I

F

F

I

T

T

-

E

-

N

-

F

K

K

D

A

C

S

S

N

L

I

N

K

I

I

G

A

V

L

K

K

R

R

M

I

V

F

S

F

G

G

K

A

F

F

L

G

N

N

A

S

G

G

Q

Q

I

I

C
|
C

I

V

T

S

S

P

D

D

Y

Y

V

L

L

L

V

V

Q

H

K

K

D

S

I

I

K

Y

E

P

K

K

F

V

L

I

T

K

Q

E

V

C

V

E

A

S

E

V

I

L

K

N

K

E

Q

F

N

Y

Y

P

S

S

I

F

-

D

E

E

N

Q

G

T

N

D

F

F

V

T

E

R

L

M

I

I

N

H

D

E

K

P

N

A

F

Y

D

K

R

K

I

A

V

V

S

A

L

S

I

I

D

N

S

S

-

T

-

N

-

G

-

S

-

K

-

I

-

V

-

P

Q

S

K

K

V

I

Y

S

Y

I

G

N

G

S

N

D

Y

-

I

T

K

E

E

D

T

L

R

C

F

L

I

V

E

P

P

P

T

T

I

I

L

V

T

Y

N

N

V

I

S

G

V

W

D

D

A

P

V

L

M

M

Q

K

E

Q

E

E

I

N

F

F

G

A

P

P

V

V

L

L

P

P

V

I

I

I

E

E

Y

Y

D

E

T

D

I

L

D

D

E

E

A

T

F

I

-

N

A

K

I

I

Q

E

K

E

F

H

E

D

K

T

P

P

L

L

S

V

A

Q

Y

Y

L

I

F

F

S

S

N

D

N

S

S

Q

T

T

I

E

K

I

K

N

R

R

F

I

L

L

N

T

E

R

V

L

S

R

F

S

G

G

N

D

G

C

A

V

I

V

N

G

D

D

S

T

I

V

M

I

Q

H

F

V

T

G

N

I

H

T

N

D

L

A

P

P

F

F

G

G

V

I

G

G

N

T

S

S

G

G

T

Y

G

G

S

N

Y

Y

H

G

G

G

K

Y

F

Y

S

G

F

F

D

N

C

T

F

F

S

S

H

H

A

E

K

R

S

T

I

I

L

F

S

K

K

Q

S

P

T

Y

W

W

I

N

E

D

A

F

N

T

L

L

-

F

-

M

K

R

Y

Y

Y

P

E

P

H

N

T

S

D

A

T

Q

K

K

M

E

K

K

I

L

I

V

K

R

S241 (≠ N230) mutation to L: Causes Q deficiency.
C273 (= C259) mutation to S: Abolishes catalytic activity.

6vr6D Structure of aldh9a1 complexed with NAD+ in space group p1 (see paper)
29% identity, 96% coverage: 2:450/469 of query aligns to 33:491/493 of 6vr6D

query
sites
6vr6D

P

P

N

A

T

T

I

G

K

R

K

V

T

I

D

A

T

T

E

F

I

T

T

C

S

S

F

G

E

E

T

K

E

E

V

V

K

N

A

L

L

A

I

V

D

Q

T

N

Q

A

R

K

K

A

F

A

F

F

Y

K

S

I

N

W

A

S

T

Q

K

K

P

S

V

G

S

M

F

E

R

R

I

C

A

R

Q

I

L

L

K

L

K

E

L

A

K

A

Q

R

L

I

K

R

D

E

N

R

E

E

A

D

Q

E

L

I

H

A

K

T

A

M

I

E

Y

C

A

I

D

N

L

N

R

G

K

K

S

S

E

I

F

F

D

E

N

A

L

R

T

L

T

-

E

-

L

-

P

-

L

-

Y

-

S

-

E

D

I

I

D

D

Y

I

A

S

V

W

K

Q

N

C

L

L

K

E

K

Y

W

Y

A

A

T

G

-

L

P

A

K

A

R

S

V

M

K

A

T

G

T

E

I

H

L

I

N

Q

F

L

P

P

S

G

K

G

S

S

-

F

-

G

Y

Y

I

T

V

R

A

R

E

E

P

P

L

L

G

G

V

V

S

C

L

V

I

G

I
|
I

G
|
G

A

A

W
|
W

N
|
N

F

Y

P

P

Y

F

N

Q

I

I

A

A

L

S

I

W

P

K

I

S

V

A

G

P

A

A

I

L

A

A

A

C

G

G

N

N

T

A

S

M

I

V

L

F

K
|
K

P

P

S

S

E

P

M

F

A

T

P

P

A

V

T

S

S

A

A

L

I

L

M

L

A

A

E

E

L

I

I

Y

N

S

K

E

-

A

N

G

F

V

P

P

S

P

N

G

Y

L

L

F

K

N

V

V

L

V

Q

Q

G

G

I
x
A

P

-

E

A

T

T

T
x
G

A
x
Q

I

F

L

L

K

C

Q

Q

R

H

F

P

D

D

-

V

-

A

K

K

I

V

F

S

F
|
F

T

T

G
|
G

S
|
S

P

V

Q

P

V
x
T

G

G

K

M

I

K

V
x
I

N

M

Q

E

A

M

A

S

A

A

P

K

H

G

L

I

T

K

N

P

V

V

T

T

L

L

E
|
E

L

L

G

G

K

K

N

S

P

P

A

L

I

I

F

I

T

F

K

S

D

D

C

C

S

D

L

M

N

N

I

N

G

A

V

V

K

K

R

G

M

A

V

L

S

M

G

A

K

N

F

F

L

L

N

T

A

Q

G

G

Q

Q

I

V

C
|
C

I

C

T

N

S

G

D

T

Y

R

V

V

L

F

V

V

Q

Q

K

K

D

E

I

I

K

L

E

D

K

K

F

F

L

T

T

E

Q

E

V

V

A

K

E

Q

-

T

-

Q

-

R

I

I

K

K

K

I

Q

G

N

D

Y

P

S

L

I

L

E

E

N

D

G

T

N

R

F

M

V

G

E

P

L

L

I

I

N

N

D

R

K

P

N

H

F

L

D

E

R

R

I

V

V

L

S

G

L

F

I

V

-

K

-

V

-

A

-

K

-

E

D

Q

S

G

Q

A

K

K

V

V

Y

L

Y

C

G

G

N

D

Y

I

-

Y

-

V

-

P

-

E

-

D

-

P

-

K

I

L

K

K

E

D

T

G

R

Y

F

Y

I

M

E

R

P

P

T

C

I

V

L

L

T

T

N

N

V

C

S

R

V

D

D

D

D

M

A

T

V

C

M

V

Q

K

E

E

I

I

F

F

G

G

P

P

V

V

L

M

P

S

V

I

I

L

E

S

Y

F

D

D

T

T

I

E

D

A

E

E

A

V

F

L

A

E

I

R

I

A

Q

N

K

D

F

T

E

T

K

F

P

G

L

L

S

A

A

A

Y

G

L

V

F

F

S

T

N

R

N

D

S

I

T

Q

I

R

K

A

K

H

R

R

F

V

L

V

N

A

E

E

V

L

S

Q

F

A

G

G

N

T

G

C

A

F

I

I

N

N

D

N

S

-

I

-

M

Y

Q

N

F

V

T

S

N

P

H

V

N

E

L

L

P

P

F

F

G

G

V

Y

G

K

N

K

S

S

G

G

T

F

G

G

S

R

Y
x
E

H

N

G

G

K

R

F

V

S

T

F

I

D

E

C

Y

F

Y

S

S

H

Q

A

L

K

K

S

T

I

V

L

C

S

V

K

E

S

M

T

G

W

D

I

V

E

E

A

S

active site: N156 (= N130), E253 (= E225), C287 (= C259), E467 (≠ Y426)
binding nicotinamide-adenine-dinucleotide: I152 (= I126), G153 (= G127), W155 (= W129), K179 (= K153), A212 (≠ I185), G215 (≠ T189), Q216 (≠ A190), F229 (= F201), G231 (= G203), S232 (= S204), T235 (≠ V207), I239 (≠ V211)

P49189 4-trimethylaminobutyraldehyde dehydrogenase; TMABA-DH; TMABALDH; Aldehyde dehydrogenase E3 isozyme; Aldehyde dehydrogenase family 9 member A1; Formaldehyde dehydrogenase; Gamma-aminobutyraldehyde dehydrogenase; R-aminobutyraldehyde dehydrogenase; EC 1.2.1.47; EC 1.2.1.3; EC 1.2.1.46; EC 1.2.1.19 from Homo sapiens (Human) (see 2 papers)
29% identity, 96% coverage: 2:450/469 of query aligns to 34:492/494 of P49189

query
sites
P49189

P

P

N

A

T

T

I

G

K

R

K

V

T

I

D

A

T

T

E

F

I

T

T

C

S

S

F

G

E

E

T

K

E

E

V

V

K

N

A

L

L

A

I

V

D

Q

T

N

Q

A

R

K

K

A

F

A

F

F

Y

K

S

I

N

W

A

S

T

Q

K

K

P

S

V

G

S

M

F

E

R

R

I

C

A

R

Q

I

L

L

K

L

K

E

L

A

K

A

Q

R

L

I

K

R

D

E

N

R

E

E

A

D

Q

E

L

I

H

A

K

T

A

M

I

E

Y

C

A

I

D

N

L

N

R

G

K

K

S

S

E

I

F

F

D

E

N

A

L

R

T

L

T

-

E

-

L

-

P

-

L

-

Y

-

S

-

E

D

I

I

D

D

Y

I

A

S

V

W

K

Q

N
x
C

L

L

K

E

K

Y

W

Y

A

A

T

G

-

L

P

A

K

A

R

S

V

M

K

A

T

G

T

E

I

H

L

I

N

Q

F

L

P

P

S

G

K

G

S

S

-

F

-

G

Y

Y

I

T

V

R

A

R

E

E

P

P

L

L

G

G

V

V

S

C

L

V

I

G

I

I

G

G

A

A

W

W

N

N

F

Y

P

P

Y

F

N

Q

I

I

A

A

L

S

I

W

P

K

I

S

V

A

G

P

A

A

I

L

A

A

A

C

G

G

N

N

T

A

S

M

I

V

L

F

K

K

P

P

S

S

E

P

M

F

A

T

P

P

A

V

T

S

S

A

A

L

I

L

M

L

A

A

E

E

L

I

I

Y

N

S

K

E

-

A

N

G

F

V

P

P

S

P

N

G

Y

L

L

F

K

N

V

V

L

V

Q

Q

G

G

I

A

P

-

E

A

T

T

T

G

A

Q

I

F

L

L

K

C

Q

Q

R

H

F

P

D

D

-

V

-

A

K

K

I

V

F

S

F

F

T

T

G

G

S

S

P

V

Q

P

V

T

G

G

K

M

I

K

V

I

N

M

Q

E

A

M

A

S

A

A

P

K

H

G

L

I

T

K

N

P

V

V

T

T

L

L

E

E

L

L

G

G

K

K

N

S

P

P

A

L

I

I

F

I

T

F

K

S

D

D

C

C

S

D

L

M

N

N

I

N

G

A

V

V

K

K

R

G

M

A

V

L

S

M

G

A

K

N

F

F

L

L

N

T

A

Q

G

G

Q

Q

I

V

C

C

I

C

T

N

S

G

D

T

Y

R

V

V

L

F

V

V

Q

Q

K

K

D

E

I

I

K

L

E

D

K

K

F

F

L

T

T

E

Q

E

V

V

A

K

E

Q

-

T

-

Q

-

R

I

I

K

K

K

I

Q

G

N

D

Y

P

S

L

I

L

E

E

N

D

G

T

N

R

F

M

V

G

E

P

L

L

I

I

N

N

D

R

K

P

N

H

F

L

D

E

R

R

I

V

V

L

S

G

L

F

I

V

-

K

-

V

-

A

-

K

-

E

D

Q

S

G

Q

A

K

K

V

V

Y

L

Y

C

G

G

N

D

Y

I

-

Y

-

V

-

P

-

E

-

D

-

P

-

K

I

L

K

K

E

D

T

G

R

Y

F

Y

I

M

E

R

P

P

T

C

I

V

L

L

T

T

N

N

V

C

S

R

V

D

D

D

D

M

A

T

V

C

M

V

Q

K

E

E

I

I

F

F

G

G

P

P

V

V

L

M

P

S

V

I

I

L

E

S

Y

F

D

D

T

T

I

E

D

A

E

E

A

V

F

L

A

E

I

R

I

A

Q

N

K

D

F

T

E

T

K

F

P

G

L

L

S

A

A

A

Y

G

L

V

F

F

S

T

N

R

N

D

S

I

T

Q

I

R

K

A

K

H

R

R

F

V

L

V

N

A

E

E

V

L

S

Q

F

A

G

G

N

T

G

C

A

F

I

I

N

N

D

N

S

-

I

-

M

Y

Q

N

F

V

T

S

N

P

H

V

N

E

L

L

P

P

F

F

G

G

V

Y

G

K

N

K

S

S

G

G

T

F

G

G

S

R

Y

E

H

N

G

G

K

R

F

V

S

T

F

I

D

E

C

Y

F

Y

S

S

H

Q

A

L

K

K

S

T

I

V

L

C

S

V

K

E

S

M

T

G

W

D

I

V

E

E

A

S

C116 (≠ N92) to S: in allele ALDH9A1*2

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; alternate
2 modified: N-acetylserine; in 4-trimethylaminobutyraldehyde dehydrogenase, N-terminally processed

O14293 Putative aldehyde dehydrogenase-like protein C9E9.09c; EC 1.2.1.- from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
35% identity, 74% coverage: 96:441/469 of query aligns to 137:493/503 of O14293

query
sites
O14293

W

W

A

A

T

D

P

K

K

D

R

Y

V

G

K

Q

T

T

T

I

I

E

L

T

N

D

F

I

P

K

S

R

K

F

S

A

Y

Y

I

T

V

R

A

H

E

E

P

P

L

I

G

G

V

V

S

C

L

G

I

Q

I

I

G

I

A

P

W

W

N

N

F

F

P

P

Y

F

N

L

I

M

A

C

L

A

I

W

P

K

I

I

V

A

G

P

A

A

I

V

A

A

A

C

G

G

N

N

T

T

S

I

I

I

L

L

K

K

P

T

S

A

E

E

M

L

A

T

P

P

A

L

T

S

S

A

A

L

I

C

M

L

A

T

E

K

L

F

I

V

N

P

K

E

-

C

N

G

F

F

P

P

S

P

N

G

Y

V

L

I

K

N

V

V

L

L

Q

S

G

G

-

D

G

G

I

R

P

R

E

C

T

G

T

N

A

A

I

I

L

S

K

S

Q

H

R

M

-

D

F

I

D

D

K

K

I

V

F

A

F

F

T

T

G

G

S
|
S

P

T

Q

G

V

V

G

G

K

R

I

M

V

V

N

M

Q

R

A

A

P

S

-

S

H

N

L

L

T

K

N

K

V

V

T

T

L

L

E

E

L

L

G

G

K

K

N

S

P

P

A

N

I

I

F

V

T

F

K

N

D

D

C

A

S

D

L

L

N

D

I

S

G

A

V

A

K

V

R

W

M

T

V

N

S

Y

G

G

K

I

F

F

L

Y

N

N

A

S

G

G

Q

Q

I

V

C

C

I

C

T

A

S

G

D

S

Y

R

V

V

L

Y

V

V

Q

Q

K

E

D

D

I

V

K

Y

E

D

K

E

F

F

L

I

T

K

Q

R

V

M

V

V

A

A

E

K

I

A

K

K

K

-

Q

-

N

-

Y

-

S

T

I

L

E

K

N

V

G

G

N

D

-

P

F

F

V

A

E

E

-

D

-

T

-

F

-

Q

-

G

-

A

L

Q

I

V

N

S

D

K

K

Q

N

Q

F

Y

D

E

R

R

I

I

V

V

S

S

L

Y

I

I

D

E

S

S

-

G

-

I

-

A

-

H

-

G

Q

A

K

K

V

L

Y

E

Y

I

G

G

-

K

N

R

Y

H

I

G

K

N

E

L

T

G

R

Y

F

F

I

V

E

E

P

P

T

T

I

I

L

L

T

S

N

N

V

V

S

T

V

E

D

D

D

M

A

A

V

V

M

G

Q

K

E

E

I

I

F

F

G

G

P

P

V

V

L

L

P

A

V

V

I

I

E

K

Y

F

D

K

T

T

I

I

D

E

E

E

A

A

F

I

A

R

I

R

I

G

Q

N

K

N

F

S

E

T

K

Y

P

G

L

L

S

A

A

A

Y

G

L

V

F

H

S

T

N

N

S

I

T

T

I

N

K

A

K

I

R

K

F

V

L

S

N

N

E

A

V

L

S

E

F

A

G

G

N

T

G

V

A

W

I

V

N

N

D

-

S

-

I

C

M

Y

Q

N

F

L

T

L

N

H

H

H

N

Q

L

I

P

P

F

F

G

G

V

Y

G

K

N

E

S

S

G

G

T

I

G

G

S

R

Y

E

H

L

G

G

K

S

F

Y

S

G

F

L

D

T

C

N

F

Y

S

T

H

Q

A

T

K

K

S

A

I

V

S248 (= S204) modified: Phosphoserine

Sites not aligning to the query:

501 modified: Phosphoserine

Query Sequence

>WP_010177305.1 NCBI__GCF_000255455.1:WP_010177305.1
MPNTIKKTDTEITSFETEVKALIDTQRKFFYSNATKPVSFRIAQLKKLKQLLKDNEAQLH
KAIYADLRKSEFDNLTTELLPLYSEIDYAVKNLKKWATPKRVKTTILNFPSKSYIVAEPL
GVSLIIGAWNFPYNIALIPIVGAIAAGNTSILKPSEMAPATSAIMAELINKNFPSNYLKV
LQGGIPETTAILKQRFDKIFFTGSPQVGKIVNQAAAPHLTNVTLELGGKNPAIFTKDCSL
NIGVKRMVSGKFLNAGQICITSDYVLVQKDIKEKFLTQVVAEIKKQNYSIENGNFVELIN
DKNFDRIVSLIDSQKVYYGGNYIKETRFIEPTILTNVSVDDAVMQEEIFGPVLPVIEYDT
IDEAFAIIQKFEKPLSAYLFSNNSTIKKRFLNEVSFGNGAINDSIMQFTNHNLPFGGVGN
SGTGSYHGKFSFDCFSHAKSILSKSTWIEANLKYYEHTDTKMKIIKKLF

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory