SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_010181980.1 NCBI__GCF_000255455.1:WP_010181980.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 16 hits to proteins with known functional sites (download)

4i3pA 1.96 angstrom x-ray crystal structure of 3-hydroxyanthranilate-3,4- dioxygenase bound with 3-aminosalicylic acid from cupriavidus metallidurans
44% identity, 94% coverage: 5:170/177 of query aligns to 6:170/174 of 4i3pA

query
sites
4i3pA

A

A

P

P

F

F

N

N

L

F

N

P

K

R

W

W

I

I

E

D

E

E

N

H

R

A

D

H

L

L

K

K

P

P

V

V

G

G

N

N

K

R

N

Q

L

V

Y

W

K

Q

D

D

A

S

G

-

D

D

Y

F

I

I

V

V

M

T

I

V

V

V

A

G

G

G

P

P

N

N

A

H

R
|
R

K

T

D

D

Y

Y

H
|
H

F

D

N

D

E

P

T

L

E

E

E
|
E

L

F

F

F

Y

Y

Q

Q

L

L

E

R

G

G

T

N

I

A

E

Y

V

L

H

N

I

L

Q

W

E

V

N

D

G

G

Q

R

K

R

R

E

T

R

M

A

E

D

L

L

G

K

P

E

G

G

D

D

M

I

Y

F

L

L

H

L

P

P

A

P

K

H

V

V

P

R

H
|
H

S

S

P
|
P

V

Q

R
|
R

-

P

H

E

K

A

G

G

S

S

I

A

G

C

L

L

V
|
V

I

I

E
|
E

R

R

K

Q

R

R

K

P

E

A

L

-

P

G

M

M

D

L

D

D

G

G

L

F

L

E

W

W

F

Y

C
|
C

D

D

N

A

C
|
C

N

G

T

H

K

L

L

V

H

H

E

R

V

V

Y

E

F

V

K

Q

L

L

E

K

D

S

I

I

E

V

V

T

D

D

F

L

L

P

K

P

H

L

F

F

K

E

H

S

F

F

Y

Y

G

A

N

S

E

E

T

D

L

K

R

R

T

R

C
|
C

K

P

N

H

C
|
C

N

G

E

Q

I

V

M

H

P

P

binding 3-amino-2-hydroxybenzoic acid: R47 (= R47), E57 (= E57), P97 (= P97), R99 (= R99), V108 (= V107), E110 (= E109)
binding fe (ii) ion: H51 (= H51), E57 (= E57), H95 (= H95), C125 (= C125), C128 (= C128), C162 (= C162), C165 (= C165)

4hvoA 1.75 angstrom x-ray crystal structure of cufe reconstituted 3- hydroxyanthranilate-3,4-dioxygenase from cupriavidus metallidurans (see paper)
44% identity, 94% coverage: 5:170/177 of query aligns to 6:170/174 of 4hvoA

query
sites
4hvoA

A

A

P

P

F

F

N

N

L

F

N

P

K

R

W

W

I

I

E

D

E

E

N

H

R

A

D

H

L

L

K

K

P

P

V

V

G

G

N

N

K

R

N

Q

L

V

Y

W

K

Q

D

D

A

S

G

-

D

D

Y

F

I

I

V

V

M

T

I

V

V

V

A

G

G

G

P

P

N

N

A

H

R

R

K

T

D

D

Y

Y

H
|
H

F

D

N

D

E

P

T

L

E

E

E
|
E

L

F

F

F

Y

Y

Q

Q

L

L

E

R

G

G

T

N

I

A

E

Y

V

L

H

N

I

L

Q

W

E

V

N

D

G

G

Q

R

K

R

R

E

T

R

M

A

E

D

L

L

G

K

P

E

G

G

D

D

M

I

Y

F

L

L

H

L

P

P

A

P

K

H

V

V

P

R

H
|
H

S

S

P

P

V

Q

R

R

-

P

H

E

K

A

G

G

S

S

I

A

G

C

L

L

V

V

I

I

E

E

R

R

K

Q

R

R

K

P

E

A

L

-

P

G

M

M

D

L

D

D

G

G

L

F

L

E

W

W

F

Y

C
|
C

D

D

N

A

C
|
C

N

G

T

H

K

L

L

V

H

H

E

R

V

V

Y

E

F

V

K

Q

L

L

E

K

D

S

I

I

E

V

V

T

D

D

F

L

L

P

K

P

H

L

F

F

K

E

H

S

F

F

Y

Y

G

A

N

S

E

E

T

D

L

K

R

R

T

R

C
|
C

K

P

N

H

C
|
C

N

G

E

Q

I

V

M

H

P

P

binding copper (ii) ion: H51 (= H51), E57 (= E57), H95 (= H95)
binding fe (ii) ion: C125 (= C125), C128 (= C128), C162 (= C162), C165 (= C165)

4hsjA 1.88 angstrom x-ray crystal structure of piconlinic-bound 3- hydroxyanthranilate-3,4-dioxygenase (see paper)
44% identity, 94% coverage: 5:170/177 of query aligns to 6:170/174 of 4hsjA

query
sites
4hsjA

A

A

P

P

F

F

N

N

L

F

N

P

K

R

W

W

I

I

E

D

E

E

N

H

R

A

D

H

L

L

K

K

P

P

V

V

G

G

N

N

K

R

N

Q

L

V

Y

W

K

Q

D

D

A

S

G

-

D

D

Y

F

I

I

V

V

M

T

I

V

V

V

A

G

G

G

P

P

N

N

A

H

R

R

K

T

D

D

Y

Y

H
|
H

F

D

N

D

E

P

T

L

E

E

E
|
E

L

F

F
|
F

Y

Y

Q

Q

L

L

E

R

G

G

T

N

I

A

E

Y

V

L

H

N

I

L

Q

W

E

V

N

D

G

G

Q

R

K

R

R

E

T

R

M

A

E

D

L

L

G

K

P

E

G

G

D

D

M

I

Y

F

L

L

H

L

P

P

A

P

K

H

V

V

P

R

H
|
H

S

S

P
|
P

V

Q

R

R

-

P

H

E

K

A

G

G

S

S

I

A

G

C

L

L

V

V

I

I

E
|
E

R

R

K

Q

R

R

K

P

E

A

L

-

P

G

M

M

D

L

D

D

G

G

L

F

L

E

W

W

F

Y

C
|
C

D

D

N

A

C
|
C

N

G

T

H

K

L

L

V

H

H

E

R

V

V

Y

E

F

V

K

Q

L

L

E

K

D

S

I

I

E

V

V

T

D

D

F

L

L

P

K

P

H

L

F

F

K

E

H

S

F

F

Y

Y

G

A

N

S

E

E

T

D

L

K

R

R

T

R

C
|
C

K

P

N

H

C
|
C

N

G

E

Q

I

V

M

H

P

P

binding pyridine-2-carboxylic acid: H51 (= H51), E57 (= E57), F59 (= F59), H95 (= H95), P97 (= P97), E110 (= E109)
binding fe (ii) ion: H51 (= H51), E57 (= E57), H95 (= H95), C125 (= C125), C128 (= C128), C162 (= C162), C165 (= C165)

1yfxA Crystal structure of 3-hydroxyanthranilate-3,4-dioxygenase from ralstonia metallidurans complexed with 4-chloro-3-hydroxyanthranilic acid and no (see paper)
44% identity, 94% coverage: 5:170/177 of query aligns to 6:170/174 of 1yfxA

query
sites
1yfxA

A

A

P

P

F

F

N

N

L

F

N

P

K

R

W

W

I

I

E

D

E

E

N

H

R

A

D

H

L

L

K

K

P

P

V
|
V

G

G

N

N

K

R

N

Q

L

V

Y

W

K

Q

D

D

A

S

G

-

D

D

Y

F

I

I

V

V

M

T

I

V

V

V

A

G

G

G

P

P

N

N

A

H

R
|
R

K

T

D

D

Y

Y

H
|
H

F

D

N

D

E

P

T

L

E

E

E
|
E

L

F

F
|
F

Y

Y

Q

Q

L

L

E

R

G

G

T

N

I

A

E

Y

V

L

H

N

I

L

Q

W

E

V

N

D

G

G

Q

R

K

R

R

E

T

R

M

A

E

D

L

L

G

K

P

E

G

G

D

D

M

I

Y

F

L

L

H

L

P

P

A

P

K

H

V

V

P

R

H
|
H

S

S

P
|
P

V

Q

R
|
R

-

P

H

E

K

A

G

G

S

S

I

A

G

C

L

L

V

V

I

I

E
|
E

R

R

K

Q

R

R

K

P

E

A

L

-

P

G

M

M

D

L

D

D

G

G

L

F

L

E

W

W

F

Y

C
|
C

D

D

N

A

C
|
C

N

G

T

H

K

L

L

V

H

H

E

R

V

V

Y

E

F

V

K

Q

L

L

E

K

D

S

I
|
I

E

V

V

T

D

D

F

L

L

P

K

P

H

L

F

F

K

E

H

S

F

F

Y

Y

G

A

N

S

E

E

T

D

L

K

R

R

T

R

C
|
C

K

P

N

H

C
|
C

N

G

E

Q

I

V

M

H

P

P

binding 4-chloro-3-hydroxyanthranilic acid: V25 (= V24), R47 (= R47), H51 (= H51), E57 (= E57), F59 (= F59), P97 (= P97), R99 (= R99), E110 (= E109), I142 (= I142)
binding fe (iii) ion: H51 (= H51), E57 (= E57), H95 (= H95), C125 (= C125), C128 (= C128), C162 (= C162), C165 (= C165)
binding nitric oxide: R47 (= R47), H51 (= H51), H95 (= H95), P97 (= P97)

1yfwA Crystal structure of 3-hydroxyanthranilate-3,4-dioxygenase from ralstonia metallidurans complexed with 4-chloro-3-hydroxyanthranilic acid and o2 (see paper)
44% identity, 94% coverage: 5:170/177 of query aligns to 6:170/174 of 1yfwA

query
sites
1yfwA

A

A

P

P

F

F

N

N

L

F

N

P

K

R

W

W

I

I

E

D

E

E

N

H

R

A

D

H

L

L

K

K

P

P

V
|
V

G

G

N

N

K

R

N

Q

L

V

Y

W

K

Q

D

D

A

S

G

-

D

D

Y

F

I

I

V

V

M

T

I

V

V

V

A

G

G

G

P

P

N

N

A

H

R
|
R

K

T

D

D

Y

Y

H
|
H

F

D

N

D

E

P

T

L

E

E

E
|
E

L

F

F
|
F

Y

Y

Q

Q

L

L

E

R

G

G

T

N

I

A

E

Y

V

L

H

N

I

L

Q

W

E

V

N

D

G

G

Q

R

K

R

R

E

T

R

M

A

E

D

L

L

G

K

P

E

G

G

D

D

M

I

Y

F

L

L

H

L

P

P

A

P

K

H

V

V

P

R

H
|
H

S

S

P
|
P

V

Q

R
|
R

-

P

H

E

K

A

G

G

S

S

I

A

G

C

L

L

V

V

I

I

E
|
E

R

R

K

Q

R

R

K

P

E

A

L

-

P

G

M

M

D

L

D

D

G

G

L

F

L

E

W

W

F

Y

C
|
C

D

D

N

A

C
|
C

N

G

T

H

K

L

L

V

H

H

E

R

V

V

Y

E

F

V

K

Q

L

L

E

K

D

S

I
|
I

E

V

V

T

D

D

F
x
L

L

P

K

P

H

L

F

F

K

E

H

S

F

F

Y

Y

G

A

N

S

E

E

T

D

L

K

R

R

T

R

C
|
C

K

P

N

H

C
|
C

N

G

E

Q

I

V

M

H

P

P

binding 4-chloro-3-hydroxyanthranilic acid: V25 (= V24), H51 (= H51), E57 (= E57), F59 (= F59), P97 (= P97), R99 (= R99), E110 (= E109), I142 (= I142), L146 (≠ F146)
binding fe (iii) ion: H51 (= H51), E57 (= E57), H95 (= H95), C125 (= C125), C128 (= C128), C162 (= C162), C165 (= C165)
binding oxygen molecule: R47 (= R47), H51 (= H51), H95 (= H95), P97 (= P97)

Q1LCS4 3-hydroxyanthranilate 3,4-dioxygenase; 3-hydroxyanthranilate oxygenase; 3-HAO; 3-hydroxyanthranilic acid dioxygenase; HAD; EC 1.13.11.6 from Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34) (Ralstonia metallidurans) (see paper)
44% identity, 94% coverage: 5:170/177 of query aligns to 6:170/174 of Q1LCS4

query
sites
Q1LCS4

A

A

P

P

F

F

N

N

L

F

N

P

K

R

W

W

I

I

E

D

E

E

N

H

R

A

D

H

L

L

K

K

P

P

V

V

G

G

N

N

K

R

N

Q

L

V

Y

W

K

Q

D

D

A

S

G

-

D

D

Y

F

I

I

V

V

M

T

I

V

V

V

A

G

G

G

P

P

N

N

A

H

R
|
R

K

T

D

D

Y

Y

H

H

F

D

N

D

E

P

T

L

E

E

L

F

F

F

Y

Y

Q

Q

L

L

E

R

G

G

T

N

I

A

E

Y

V

L

H

N

I

L

Q

W

E

V

N

D

G

G

Q

R

K

R

R

E

T

R

M

A

E

D

L

L

G

K

P

E

G

G

D

D

M

I

Y

F

L

L

H

L

P

P

A

P

K

H

V

V

P

R

H

H

S

S

P

P

V

Q

R
|
R

-

P

H

E

K

A

G

G

S

S

I

A

G

C

L

L

V

V

I

I

E
|
E

R

R

K

Q

R

R

K

P

E

A

L

-

P

G

M

M

D

L

D

D

G

G

L

F

L

E

W

W

F

Y

C

C

D

D

N

A

C

C

N

G

T

H

K

L

L

V

H

H

E

R

V

V

Y

E

F

V

K

Q

L

L

E

K

D

S

I

I

E

V

V

T

D

D

F

L

L

P

K

P

H

L

F

F

K

E

H

S

F

F

Y

Y

G

A

N

S

E

E

T

D

L

K

R

R

T

R

C

C

K

P

N

H

C

C

N

G

E

Q

I

V

M

H

P

P

R47 (= R47) mutation to A: Increases KM for 3-hydroxyanthranilate 7-fold. Decreases activity 1000-fold.
R99 (= R99) mutation to A: Increases KM for 3-hydroxyanthranilate 40-fold. Decreases activity 5000-fold.
E110 (= E109) mutation to A: Decreases KM for 3-hydroxyanthranilate 2-fold. Decreases activity 2000-fold.

6viaA Observing a ring-cleaving dioxygenase in action through a crystalline lens - a seven-membered lactone bound structure (see paper)
44% identity, 94% coverage: 5:170/177 of query aligns to 8:172/176 of 6viaA

query
sites
6viaA

A

A

P

P

F

F

N

N

L

F

N

P

K

R

W

W

I

I

E

D

E

E

N

H

R

A

D

H

L

L

K

K

P

P

V

V

G

G

N

N

K

R

N

Q

L

V

Y

W

K

Q

D

D

A

S

G

-

D

D

Y

F

I

I

V

V

M

T

I

V

V
|
V

A

G

G

G

P

P

N

N

A

H

R

R

K

T

D

D

Y

Y

H
|
H

F

D

N

D

E

P

T

L

E

E

E
|
E

L

F

F
|
F

Y

Y

Q

Q

L

L

E

R

G

G

T

N

I

A

E

Y

V

L

H

N

I

L

Q

W

E

V

N

D

G

G

Q

R

K

R

R

E

T

R

M

A

E

D

L

L

G

K

P

E

G

G

D

D

M

I

Y

F

L

L

H

L

P

P

A

P

K

H

V

V

P

R

H
|
H

S

S

P
|
P

V

Q

R
|
R

-

P

H

E

K

A

G

G

S

S

I

A

G

C

L

L

V

V

I

I

E
|
E

R

R

K

Q

R

R

K

P

E

A

L

-

P

G

M

M

D

L

D

D

G

G

L

F

L

E

W

W

F

Y

C
|
C

D

D

N

A

C
|
C

N

G

T

H

K

L

L

V

H

H

E

R

V

V

Y

E

F

V

K

Q

L

L

E

K

D

S

I

I

E

V

V

T

D

D

F

L

L

P

K

P

H

L

F

F

K

E

H

S

F

F

Y

Y

G

A

N

S

E

E

T

D

L

K

R

R

T

R

C
|
C

K

P

N

H

C
|
C

N

G

E

Q

I

V

M

H

P

P

binding (2R,3E)-2-hydroxy-3-imino-2,3-dihydrooxepine-4-carboxylic acid: V43 (= V41), H53 (= H51), E59 (= E57), F61 (= F59), P99 (= P97), R101 (= R99), E112 (= E109)
binding fe (iii) ion: H53 (= H51), E59 (= E57), H97 (= H95)
binding fe (ii) ion: C127 (= C125), C130 (= C128), C164 (= C162), C167 (= C165)

6vi8A Observing a ring-cleaving dioxygenase in action through a crystalline lens - a superoxo bound structure (see paper)
44% identity, 94% coverage: 5:170/177 of query aligns to 8:172/176 of 6vi8A

query
sites
6vi8A

A

A

P

P

F

F

N

N

L

F

N

P

K

R

W

W

I

I

E

D

E

E

N

H

R

A

D

H

L

L

K

K

P

P

V

V

G

G

N

N

K

R

N

Q

L

V

Y

W

K

Q

D

D

A

S

G

-

D

D

Y

F

I

I

V

V

M

T

I

V

V

V

A

G

G

G

P

P

N

N

A

H

R

R

K

T

D

D

Y

Y

H
|
H

F

D

N

D

E

P

T

L

E

E

E
|
E

L

F

F
|
F

Y

Y

Q

Q

L

L

E

R

G

G

T

N

I

A

E

Y

V

L

H

N

I

L

Q

W

E

V

N

D

G

G

Q

R

K

R

R

E

T

R

M

A

E

D

L

L

G

K

P

E

G

G

D

D

M

I

Y

F

L

L

H

L

P

P

A

P

K

H

V

V

P

R

H
|
H

S

S

P
|
P

V

Q

R
|
R

-

P

H

E

K

A

G

G

S

S

I

A

G

C

L

L

V

V

I

I

E
|
E

R

R

K

Q

R

R

K

P

E

A

L

-

P

G

M

M

D

L

D

D

G

G

L

F

L

E

W

W

F

Y

C
|
C

D

D

N

A

C
|
C

N

G

T

H

K

L

L

V

H

H

E

R

V

V

Y

E

F

V

K

Q

L

L

E

K

D

S

I

I

E

V

V

T

D

D

F

L

L

P

K

P

H

L

F

F

K

E

H

S

F

F

Y

Y

G

A

N

S

E

E

T

D

L

K

R

R

T

R

C
|
C

K

P

N

H

C
|
C

N

G

E

Q

I

V

M

H

P

P

binding 3-hydroxyanthranilic acid: H53 (= H51), E59 (= E57), F61 (= F59), P99 (= P97), R101 (= R99), E112 (= E109)
binding fe (iii) ion: H53 (= H51), E59 (= E57), H97 (= H95)
binding fe (ii) ion: C127 (= C125), C130 (= C128), C164 (= C162), C167 (= C165)
binding oxygen atom: H53 (= H51), H97 (= H95)

6vibA Observing a ring-cleaving dioxygenase in action through a crystalline lens - enol tautomers of acms bidentately bound structure (see paper)
44% identity, 94% coverage: 5:170/177 of query aligns to 7:171/175 of 6vibA

query
sites
6vibA

A

A

P

P

F

F

N

N

L

F

N

P

K

R

W

W

I

I

E

D

E

E

N

H

R

A

D

H

L

L

K

K

P

P

V

V

G

G

N

N

K

R

N

Q

L

V

Y

W

K

Q

D

D

A

S

G

-

D

D

Y

F

I

I

V

V

M

T

I

V

V
|
V

A

G

G

G

P

P

N

N

A

H

R

R

K

T

D

D

Y

Y

H
|
H

F

D

N

D

E

P

T

L

E

E

E
|
E

L

F

F
|
F

Y

Y

Q

Q

L

L

E

R

G

G

T

N

I

A

E

Y

V

L

H

N

I

L

Q

W

E

V

N

D

G

G

Q

R

K

R

R

E

T

R

M

A

E

D

L

L

G

K

P

E

G

G

D

D

M

I

Y

F

L

L

H

L

P

P

A

P

K

H

V

V

P

R

H
|
H

S

S

P
|
P

V

Q

R
|
R

-

P

H

E

K

A

G

G

S

S

I

A

G

C

L

L

V
|
V

I

I

E
|
E

R

R

K

Q

R

R

K

P

E

A

L

-

P

G

M

M

D

L

D

D

G

G

L

F

L

E

W

W

F

Y

C
|
C

D

D

N

A

C
|
C

N

G

T

H

K

L

L

V

H

H

E

R

V

V

Y

E

F

V

K

Q

L

L

E

K

D

S

I
|
I

E

V

V

T

D

D

F

L

L

P

K

P

H

L

F

F

K

E

H

S

F

F

Y

Y

G

A

N

S

E

E

T

D

L

K

R

R

T

R

C
|
C

K

P

N

H

C
|
C

N

G

E

Q

I

V

M

H

P

P

binding (2Z,3Z)-2-[(2Z)-3-hydroxyprop-2-en-1-ylidene]-3-iminobutanedioic acid: V42 (= V41), H52 (= H51), E58 (= E57), F60 (= F59), H96 (= H95), P98 (= P97), R100 (= R99), V109 (= V107), E111 (= E109), I143 (= I142)
binding fe (ii) ion: H52 (= H51), E58 (= E57), H96 (= H95), C126 (= C125), C129 (= C128), C163 (= C162), C166 (= C165)

6vi9A Observing a ring-cleaving dioxygenase in action through a crystalline lens - an alkylperoxo bound structure (see paper)
44% identity, 94% coverage: 5:170/177 of query aligns to 6:170/171 of 6vi9A

query
sites
6vi9A

A

A

P

P

F

F

N

N

L

F

N

P

K

R

W

W

I

I

E

D

E

E

N

H

R

A

D

H

L

L

K

K

P

P

V

V

G

G

N

N

K

R

N

Q

L

V

Y

W

K

Q

D

D

A

S

G

-

D

D

Y

F

I

I

V

V

M

T

I

V

V

V

A

G

G

G

P

P

N

N

A

H

R
|
R

K

T

D

D

Y

Y

H
|
H

F

D

N

D

E

P

T

L

E

E

E
|
E

L

F

F
|
F

Y

Y

Q

Q

L

L

E

R

G

G

T

N

I

A

E

Y

V

L

H

N

I

L

Q

W

E

V

N

D

G

G

Q

R

K

R

R

E

T

R

M

A

E

D

L

L

G

K

P

E

G

G

D

D

M

I

Y

F

L

L

H

L

P

P

A

P

K

H

V

V

P

R

H
|
H

S

S

P
|
P

V

Q

R
|
R

-

P

H

E

K

A

G

G

S

S

I

A

G

C

L

L

V

V

I

I

E
|
E

R

R

K

Q

R

R

K

P

E

A

L

-

P

G

M

M

D

L

D

D

G

G

L

F

L

E

W

W

F

Y

C
|
C

D

D

N

A

C
|
C

N

G

T

H

K

L

L

V

H

H

E

R

V

V

Y

E

F

V

K

Q

L

L

E

K

D

S

I

I

E

V

V

T

D

D

F

L

L

P

K

P

H

L

F

F

K

E

H

S

F

F

Y

Y

G

A

N

S

E

E

T

D

L

K

R

R

T

R

C
|
C

K

P

N

H

C
|
C

N

G

E

Q

I

V

M

H

P

P

binding fe (ii) ion: C125 (= C125), C128 (= C128), C162 (= C162), C165 (= C165)
binding (5R,6Z)-5-(hydroperoxy-kappaO)-5-(hydroxy-kappaO)-6-iminocyclohexa-1,3-diene-1-carboxylato(2-)iron: R47 (= R47), H51 (= H51), E57 (= E57), F59 (= F59), H95 (= H95), P97 (= P97), R99 (= R99), E110 (= E109)

4wzcA Understanding extradiol dioxygenase mechanism in NAD+ biosynthesis by viewing catalytic intermediates - 2,3-cis-4,5-trans acms bound to i142a mutant hao
43% identity, 94% coverage: 5:170/177 of query aligns to 6:170/174 of 4wzcA

query
sites
4wzcA

A

A

P

P

F

F

N

N

L

F

N

P

K

R

W

W

I

I

E

D

E

E

N

H

R

A

D

H

L

L

K

K

P

P

V

V

G

G

N

N

K

R

N

Q

L

V

Y

W

K

Q

D

D

A

S

G

-

D

D

Y

F

I

I

V

V

M

T

I

V

V

V

A

G

G

G

P

P

N

N

A

H

R
|
R

K

T

D

D

Y

Y

H
|
H

F

D

N

D

E

P

T

L

E

E

E
|
E

L

F

F

F

Y

Y

Q

Q

L

L

E

R

G

G

T

N

I

A

E

Y

V

L

H

N

I

L

Q

W

E

V

N

D

G

G

Q

R

K

R

R

E

T

R

M

A

E

D

L

L

G

K

P

E

G

G

D

D

M

I

Y

F

L

L

H

L

P

P

A

P

K

H

V

V

P

R

H
|
H

S

S

P
|
P

V

Q

R
|
R

-

P

H

E

K

A

G

G

S

S

I

A

G

C

L

L

V

V

I

I

E
|
E

R

R

K

Q

R

R

K

P

E

A

L

-

P

G

M

M

D

L

D

D

G

G

L

F

L

E

W

W

F

Y

C
|
C

D

D

N

A

C
|
C

N

G

T

H

K

L

L

V

H

H

E

R

V

V

Y

E

F

V

K

Q

L

L

E

K

D

S

I

A

E

V

V

T

D

D

F

L

L

P

K

P

H

L

F

F

K

E

H

S

F

F

Y

Y

G

A

N

S

E

E

T

D

L

K

R

R

T

R

C
|
C

K

P

N

H

C
|
C

N

G

E

Q

I

V

M

H

P

P

binding (2E)-2-amino-3-[(1E)-3-oxoprop-1-en-1-yl]but-2-enedioic acid: R47 (= R47), H51 (= H51), E57 (= E57), H95 (= H95), P97 (= P97), R99 (= R99), E110 (= E109)
binding fe (ii) ion: H51 (= H51), E57 (= E57), H95 (= H95), C125 (= C125), C128 (= C128), C162 (= C162), C165 (= C165)

Q83V26 3-hydroxyanthranilate 3,4-dioxygenase; 3-hydroxyanthranilate oxygenase; 3-HAO; 3-hydroxyanthranilic acid dioxygenase; HAD; EC 1.13.11.6 from Pseudomonas fluorescens (see paper)
42% identity, 93% coverage: 6:170/177 of query aligns to 8:171/185 of Q83V26

query
sites
Q83V26

P

P

F

L

N

N

L

F

N

Q

K

R

W

W

I

L

E

D

N

H

R

S

D

D

L

L

K

R

P

P

V

V

G

G

N

N

K

Q

N

Q

L

V

Y

W

K

Q

D

D

A

S

G

-

D

D

Y

F

I

I

V

V

M

T

I

V

V

V

A

G

G

G

P

P

N

N

A

F

R

R

K

T

D

D

Y

F

H
|
H

F

D

N

D

E

P

T

M

E

E

L

F

F

F

Y

Y

Q

Q

L

F

E

K

G

G

T

N

I

A

E

Y

V

L

H

N

I

I

Q

M

E

D

N

R

G

G

Q

Q

K

M

R

D

T

R

M

V

E

E

L

L

G

K

P

E

G

G

D

D

M

I

Y

F

L

L

H

L

P

P

A

P

K

H

V

L

P

R

H
|
H

S

S

P

P

V

Q

R

R

-

P

H

E

K

A

G

G

S

S

I

R

G

C

L

L

V

V

I

I

E

E

R

R

K

Q

R

R

K

P

E

K

L

-

P

G

M

M

D

L

D

D

G

G

L

F

L

E

W

W

F

Y

C

C

D

L

N

S

C

C

N

N

T

G

K

L

L

V

H

Y

E

R

V

V

Y

D

F

V

K

Q

L

L

E

N

D

S

I

I

E

V

V

T

D

D

F

L

L

P

K

P

H

L

F

F

K

D

H

I

F

F

Y

Y

G

G

N

N

E

V

T

G

L

L

R

R

T

K

C

C

K

P

N

Q

C

C

N

G

E

Q

I

V

M

H

P

P

H52 (= H51) mutation to A: Loss of activity.
H96 (= H95) mutation to A: Loss of activity.

3fe5A Crystal structure of 3-hydroxyanthranilate 3,4-dioxygenase from bovine kidney (see paper)
41% identity, 89% coverage: 6:163/177 of query aligns to 3:157/283 of 3fe5A

query
sites
3fe5A

P

P

F

V

N

R

L

V

N

K

K

A

W

W

I

V

E

E

N

N

R

R

D

G

L

S

L

F

K

L

P

P

V

V

G

C

N

N

K

K

N

L

L

L

Y

H

K

Q

D

K

A

-

G

-

D

Q

Y

L

I

K

V

I

M

M

I

F

V

V

A

G

G

G

P

P

N

N

A

T

R

R

K

K

D

D

Y

Y

H
|
H

F

I

N

E

E

E

T

G

E

E

E
|
E

L

V

F

F

Y

Y

Q

Q

L

L

E

E

G

G

T

D

I

M

E

L

V

L

H

R

I

V

Q

L

E

E

N

R

G

G

Q

K

K

H

R

R

T

D

M

V

E

V

L

I

G

R

P

Q

G

G

D

E

M

I

Y

F

L

L

H

L

P

P

A

A

K

G

V

V

P

P

H
|
H

S

S

P

P

V

Q

R

R

H

F

K

A

G

N

S

T

I

V

G

G

L

L

V

V

I

I

E

E

R

R

K

R

R

R

K

L

E

K

L

T

P

E

M

L

D

-

D

D

G

G

L

L

L

R

W

Y

F

Y

C

V

D

G

N

D

C

T

N

T

T

D

K

V

L

L

H

F

E

E

V

K

Y

W

F

F

K

Y

L

C

E

E

D

D

I

L

E

G

V

T

D

Q

F

L

L

A

K

P

H

I

F

I

K

Q

H

E

F

F

Y

F

G

S

N

S

E

E

T

Q

L

Y

R

R

T

T

C

G

K

K

binding fe (iii) ion: H46 (= H51), E52 (= E57), H90 (= H95)

5tkqA Crystal structure of human 3hao with zinc bound in the active site (see paper)
38% identity, 89% coverage: 7:163/177 of query aligns to 3:156/283 of 5tkqA

query
sites
5tkqA

F

L

N

G

L

V

N

R

K

A

W

W

I

V

E

K

E

E

N

N

R

R

D

G

L

S

L

F

K

Q

P

P

V

V

G

C

N

N

K

K

N

L

L

M

Y

H

K

Q

D

E

A

-

G

-

D

Q

Y

L

I

K

V

V

M

M

I

F

V

I

A

G

G

G

P

P

N

N

A

T

R

R

K

K

D

D

Y

Y

H
|
H

F

I

N

E

E

E

T

G

E

E

E
|
E

L

V

F

F

Y

Y

Q

Q

L

L

E

E

G

G

T

D

I

M

E

V

V

L

H

R

I

V

Q

L

E

E

N

Q

G

G

Q

K

K

H

R

R

T

D

M

V

E

V

L

I

G

R

P

Q

G

G

D

E

M

I

Y

F

L

L

H

L

P

P

A

A

K

R

V

V

P

P

H
|
H

S

S

P

P

V

Q

R

R

H

F

K

A

G

N

S

T

I

V

G

G

L

L

V

V

I

V

E

E

R

R

K

R

R

R

K

L

E

E

L

T

P

E

M

L

D

-

D

D

G

G

L

L

L

R

W

Y

F

Y

C

V

D

G

N

D

C

T

N

M

T

D

K

V

L

L

H

F

E

E

V

K

Y

W

F

F

K

Y

L

C

E

K

D

D

I

L

E

G

V

T

D

Q

F

L

L

A

K

P

H

I

F

I

K

Q

H

E

F

F

Y

F

G

S

N

S

E

E

T

Q

L

Y

R

R

T

T

C

G

K

K

binding zinc ion: H45 (= H51), E51 (= E57), H89 (= H95)

5tk5A Crystal structure of human 3hao with iron bound in the active site (see paper)
38% identity, 89% coverage: 7:163/177 of query aligns to 3:156/283 of 5tk5A

query
sites
5tk5A

F

L

N

G

L

V

N

R

K

A

W

W

I

V

E

K

E

E

N

N

R

R

D

G

L

S

L

F

K

Q

P

P

V

V

G

C

N

N

K

K

N

L

L

M

Y

H

K

Q

D

E

A

-

G

-

D

Q

Y

L

I

K

V

V

M

M

I

F

V

I

A

G

G

G

P

P

N

N

A

T

R

R

K

K

D

D

Y

Y

H
|
H

F

I

N

E

E

E

T

G

E

E

E
|
E

L

V

F

F

Y

Y

Q

Q

L

L

E

E

G

G

T

D

I

M

E

V

V

L

H

R

I

V

Q

L

E

E

N

Q

G

G

Q

K

K

H

R

R

T

D

M

V

E

V

L

I

G

R

P

Q

G

G

D

E

M

I

Y

F

L

L

H

L

P

P

A

A

K

R

V

V

P

P

H
|
H

S

S

P

P

V

Q

R

R

H

F

K

A

G

N

S

T

I

V

G

G

L

L

V

V

I

V

E

E

R

R

K

R

R

R

K

L

E

E

L

T

P

E

M

L

D

-

D

D

G

G

L

L

L

R

W

Y

F

Y

C

V

D

G

N

D

C

T

N

M

T

D

K

V

L

L

H

F

E

E

V

K

Y

W

F

F

K

Y

L

C

E

K

D

D

I

L

E

G

V

T

D

Q

F

L

L

A

K

P

H

I

F

I

K

Q

H

E

F

F

Y

F

G

S

N

S

E

E

T

Q

L

Y

R

R

T

T

C

G

K

K

binding fe (iii) ion: H45 (= H51), E51 (= E57), H89 (= H95)

P46952 3-hydroxyanthranilate 3,4-dioxygenase; 3-hydroxyanthranilate oxygenase; 3-HAO; h3HAO; 3-hydroxyanthranilic acid dioxygenase; HAD; EC 1.13.11.6 from Homo sapiens (Human) (see 3 papers)
38% identity, 89% coverage: 7:163/177 of query aligns to 5:158/286 of P46952

query
sites
P46952

F

L

N

G

L

V

N

R

K

A

W

W

I

V

E

K

E

E

N

N

R

R

D

G

L

S

L

F

K

Q

P

P

V

V

G

C

N

N

K

K

N

L

L

M

Y

H

K

Q

D

E

A

-

G

-

D

Q

Y

L

I

K

V

V

M

M

I

F

V
x
I

A

G

G

G

P

P

N

N

A

T

R

R

K

K

D

D

Y

Y

H
|
H

F

I

N

E

E

E

T

G

E

E

E
|
E

L

V

F

F

Y

Y

Q

Q

L

L

E

E

G

G

T

D

I

M

E

V

V

L

H

R

I

V

Q

L

E

E

N

Q

G

G

Q

K

K

H

R

R

T

D

M

V

E

V

L

I

G

R

P

Q

G

G

D

E

M

I

Y

F

L

L

H

L

P

P

A

A

K

R

V

V

P

P

H
|
H

S

S

P

P

V

Q

R

R

H

F

K

A

G

N

S

T

I

V

G

G

L

L

V

V

I

V

E

E

R

R

K

R

R

R

K

L

E

E

L

T

P

E

M

L

D

-

D

D

G

G

L

L

L

R

W

Y

F

Y

C

V

D

G

N

D

C

T

N

M

T

D

K

V

L

L

H

F

E

E

V

K

Y

W

F

F

K

Y

L

C

E

K

D

D

I

L

E

G

V

T

D

Q

F

L

L

A

K

P

H

I

F

I

K

Q

H

E

F

F

Y

F

G

S

N

S

E

E

T

Q

L

Y

R

R

T

T

C

G

K

K

I37 (≠ V41) to V: in dbSNP:rs3816183
H47 (= H51) binding Fe cation
E53 (= E57) binding Fe cation
H91 (= H95) binding Fe cation

Sites not aligning to the query:

162:286 natural variant: Missing (in VCRL1; strongly reduced 3-hydroxyanthranilate 3,4-dioxygenase activity in vitro)
186:286 natural variant: Missing (in VCRL1; strongly reduced 3-hydroxyanthranilate 3,4-dioxygenase activity in vitro)

Query Sequence

>WP_010181980.1 NCBI__GCF_000255455.1:WP_010181980.1
MKIQAPFNLNKWIEENRDLLKPPVGNKNLYKDAGDYIVMIVAGPNARKDYHFNETEELFY
QLEGTIEVHIQENGQKRTMELGPGDMYLHPAKVPHSPVRHKGSIGLVIERKRKELPMDDG
LLWFCDNCNTKLHEVYFKLEDIEVDFLKHFKHFYGNETLRTCKNCNEIMPVDERFVK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory