SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_010438514.1 NCBI__GCF_000192475.1:WP_010438514.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

7cagA Mycobacterium smegmatis lpqy-sugabc complex in the catalytic intermediate state (see paper)
30% identity, 80% coverage: 6:236/290 of query aligns to 6:233/285 of 7cagA

query
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7cagA

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binding alpha-D-glucopyranose: E159 (≠ A162), K162 (≠ Q165), R216 (≠ L219), D219 (≠ L222)

4ki0F Crystal structure of the maltose-binding protein/maltose transporter complex in an outward-facing conformation bound to maltohexaose (see paper)
24% identity, 86% coverage: 29:278/290 of query aligns to 223:488/490 of 4ki0F

query
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4ki0F

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binding alpha-D-glucopyranose: Y310 (≠ M110), N361 (≠ A162), G365 (≠ S166), Y368 (≠ F169), S418 (≠ A215), N422 (≠ A223)

P02916 Maltose/maltodextrin transport system permease protein MalF from Escherichia coli (strain K12) (see 4 papers)
24% identity, 82% coverage: 40:278/290 of query aligns to 249:503/514 of P02916

query
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P02916

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L334 (≠ T119) mutation to W: Ability to transport lactose in a saturable manner.
L372 (≠ I158) mutation to W: Growth on maltose but not on media containing either maltoheptaose or maltoheptaose plus maltose.
N376 (≠ A162) mutation N->K,H: No growth on maltose.
G380 (≠ S166) mutation G->C,S: No growth on maltose.
E401 (≠ K187) mutation E->A,C,K,L: Reduction of transport rate.
S403 (≠ A189) mutation S->C,D,K,L: Reduction of transport rate.
G407 (= G193) mutation G->A,P: No effect.
P420 (= P206) mutation to A: No effect.

P94529 Arabinooligosaccharides transport system permease protein AraP from Bacillus subtilis (strain 168) (see paper)
24% identity, 79% coverage: 37:264/290 of query aligns to 57:286/313 of P94529

query
sites
P94529

L

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A

E205 (= E184) mutation to A: Has no effect on the growth rate.
E208 (≠ K187) mutation to A: Small negative impact in the uptake of arabinotriose. Shows an increase of about 10% in the doubling time.
D213 (= D192) mutation to A: Severely impairs the uptake of arabinotriose. Shows a 3.6-fold increase of the doubling time.

Query Sequence

>WP_010438514.1 NCBI__GCF_000192475.1:WP_010438514.1
MSEWLENHLPKVVLAPSFIAVLIFVYGFIGWTAWVSLTRSKLLPKYEIKGFIQYERLFDS
PRWDTAINNLYIFGALFIVIAMVLGLLLAILLDQKIRVEGAIRTIYLYPMALSMIVTGTA
WKWILNPGLGIESMVQGWGFANFEFDWLVNPDYAIYTIVMAAIWQSSGFVMALFLAGLRS
VDGEIIKAAQVDGIPTWRIYTAIIIPSMAPIFLSAFIVLAHLAIKSFDLVIALTGGGPGY
ATDLPATYMYAMAFSRGDIGQAASSAMIMMLVVFAIVVPYLYSELRAKND

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory