SitesBLAST

G51 (= G83) mutation to S: Shows 4.3- and 107-fold increase of affinity to glyphosate and glycine, respectively. Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with R-54, R-81, C-202, V-332 and V-342.
A54 (≠ Q86) mutation to R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-81, C-202, V-332 and V-342.
K81 (= K113) mutation to R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, C-202, V-332 and V-342.
S202 (≠ C244) mutation to C: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, V-332 and V-342.
I332 (≠ V376) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-342.
M342 (= M386) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-332.

7cyxA Crystal strcuture of glycine oxidase from bacillus cereus atcc 14579 (see paper)
22% identity, 82% coverage: 38:395/434 of query aligns to 3:349/363 of 7cyxA

query
sites
7cyxA

D

D

V

V

C

A

I

I

V
x
I

G
|
G

G

G

G
|
G

Y
x
V

T
x
I

G

G

L

S

S

S

A

V

A

A

L

H

H

F

L

L

A

A

E

E

A

R

G

G

F

H

D

K

V

V

V

A

L

I

L
x
V

E
|
E

A
x
K

H

Q

R

S

A

I

G

A

F

S

G
x
E

A
|
A

S
|
S

G

K

R

A

N
x
A

G

A

G
|
G

Q
x
L

L

L

G

G

S

V

A

A

Q

Y

R

N

M

-

D

-

Q

-

E

-

D

-

L

-

E

-

R

-

L

-

V

-

G

-

D

-

A

-

L

-

K

P

L

L

W

F

D

E

L

L

A

A

E

R

D

E

A

S

K

R

D

A

L

I

V

F

K

P

S

Q

L

L

-

A

I

V

S

L

K

R

H

E

Q

K

I

T

D

G

C

V

D

D

L

I

-

G

-

Y

-

E

K

E

P

K

G

G

I

I

A

Y

V

R

L

I

G

A

F

Q

N

N

D

E

S

D

E

E

R

K

A

E

E

R

L

I

H

L

D

-

H

H

A

I

A

M

H

D

L

W

S

Q

G

Q

R

K

Y

T

K

G

Y

E

D

D

Q

S

I

Y

E

-

A

F

L

L

D

T

A

G

D

D

A

H

A

V

R

R

G

E

L

K

C

E

P

P

-

Y

-

L

S

S

P

E

A

S

Y

I

A

I

G

G

G

A

Y

V

L

Y

D

Y

N

P

G

K

A

D

A

G

H

H

L

V

H

I

P

A

L

P

N

E

Y

L

A

T

L

K

G

A

L

F

A

A

R

H

A

S

A

A

V

A

G

I

A

S

G

G

L

A

R

D

L

I

H

Y

E

E

D

Q

T

T

E

E

V
|
V

L

F

G

D

I

I

D

R

-

I

Q

E

G

N

A

N

R

K

V

V

T

T

-

G

V

V

R

I

T

T

E

S

K

E

G

G

K

I

I

V

T

T

A

C

D

E

N

K

V

V

I

V

L

I

A

A

C
x
G

N
x
G

G

S

Y
x
W

L

-

G

-

D

-

L

-

N

-

R

-

Q

-

V

-

A

S

S

T

R

K

V

L

M

L

P

S

I

Y

N

F

N

H

F

R

I

D

A

W

A

G

T

T

E

Y

P

P

L

V

G

K

A

G

E

E

A

V

Q

V

K

A

V

V

L

R

A

S

K

R

D

K

I

Q

A

L

V

L

A

K

D

A

T

P

K

I

F

F

V

Q

V

E

N

R

Y

F

F

Y

R

I

L

T

S

P

A

K

D

R

G

G

R

G

L

R

L

Y

F

V

G

I

G

G

G

A

E

T

S

M

Y

K

G

P

Y

H

R

T

F

F

-

N

-

K

-

T

-

V

-

Q

P

P

E

E

D

S

I

I

A

T

A

S

K

I

V

L

R

E

K

R

P

A

M

Y

T

T

E

-

I

I

Y

L

P

P

H

A

L

L

R

K

D

E

V

A

K

E

I

W

D

E

Y

S

A

T

W

W

G

A

G
|
G

T

L

L
x
R

G
x
P

I

Q

T

S

M

N

R

H

R

E

M

A

P

P

Y

Y

L

M

A

G

R

E

I

H

A

E

-

E

-

I

P

K

N

G

I

L

L

Y

S

A

A

C

S

T

G

G

Y

H

S
x
Y

G
x
R

H
x
N

G
|
G

V
x
I

G

L

T

L

A

S

T

P

H

I

A

S

G

G

Q

Q

L

Y

M

M

A

A

L

D

A

L

I

I

Q

E

G

G

Q

K

A

Q

E

E

binding flavin-adenine dinucleotide: I7 (≠ V42), G8 (= G43), G10 (= G45), V11 (≠ Y46), I12 (≠ T47), V30 (≠ L65), E31 (= E66), K32 (≠ A67), E38 (≠ G73), A39 (= A74), S40 (= S75), A43 (≠ N78), G45 (= G80), L46 (≠ Q81), V171 (= V217), G200 (≠ C244), G201 (≠ N245), W203 (≠ Y247), G298 (= G346), R300 (≠ L348), P301 (≠ G349), Y326 (≠ S372), R327 (≠ G373), N328 (≠ H374), G329 (= G375), I330 (≠ V376)

3hzlA Tyr258phe mutant of nikd, an unusual amino acid oxidase essential for nikkomycin biosynthesis: open form at 1.55a resolution (see paper)
28% identity, 49% coverage: 49:259/434 of query aligns to 16:218/394 of 3hzlA

query
sites
3hzlA

L

L

S

A

A

T

A

A

L

W

H

Q

L

V

A

A

E

E

A

R

G

G

F

H

D

R

V

V

V

L

L

V

L
|
L

E
|
E

A
x
R

H

H

R

T

A

F

G

F

F

N

G

E

A

N

S

G

G
|
G

R
x
T

N
x
S

G

G

G

A

Q
x
E

L
x
R

G
x
H

S

W

A
x
R

Q

L

R

Q

M

Y

D

T

Q

Q

E

E

D

D

L

L

E

F

R

R

L

L

V

T

G

L

D

-

D

E

A

T

L

L

K

P

L

L

W

W

D

R

L

A

A

L

E

E

D

S

A

R

K

C

D

E

L

-

V

-

K

R

S

R

L

L

I

I

S

H

K

E

H

-

Q

-

I

-

D

-

C

-

D

-

L

-

K

-

P

-

G

-

I

I

A

G

V

S

L

L

G

W

F

F

N

G

D

D

S

T

E

D

-

V

-

T

-

N

R
x
E

A

G

E

Q

L

I

H

S

D

G

H

T

A

A

H

M

L

M

S

D

G

K

-

L

R

S

Y

V

K

R

Y

Y

D

E

Q

W

I

L

E

K

A

A

L

T

D

D

A

I

D

E

A

R

R

F

G

G

L

F

C

R

P

G

S

L

P

P

A

R

Y

D

A

Y

G

E

G

G

Y

F

L

L

-

Q

-

P

D

D

N

G

G

G

A

T

A

I

H

D

L

V

H

R

P

G

L

T

N

L

Y

A

A

A

L

L

G

F

L

T

A

L

R

A

A

Q

A

A

V

A

G

G

A

A

G

T

L

L

R

R

L

A

H

G

E

E

D

T

T
x
V

E

T

V

E

L

L

G

V

I

P

D

D

Q

A

G

D

A

G

R

-

V

V

T

S

V

V

R

T

T

T

E

D

K

R

G

G

K

T

I

Y

T

R

A

A

D

G

N

K

V

V

I

V

L

L

A

A

C
|
C

N

G

G

P

Y
|
Y

L

T

G

N

D

D

L

L

N

L

R

E

Q

P

V

L

A

G

S

A

R

R

V

L

active site: S45 (≠ N78), E48 (≠ Q81), R49 (≠ L82), R52 (≠ A85), E100 (≠ R143)
binding pyridine-2-carboxylic acid: R52 (≠ A85), E100 (≠ R143)
binding flavin-adenine dinucleotide: L32 (= L65), E33 (= E66), R34 (≠ A67), G43 (= G76), T44 (≠ R77), R49 (≠ L82), H50 (≠ G83), V175 (≠ T215), C203 (= C244), Y206 (= Y247)

Sites not aligning to the query:

active site: 257, 271, 275, 304, 320, 354, 357
binding pyridine-2-carboxylic acid: 241, 257, 354
binding flavin-adenine dinucleotide: 10, 12, 13, 14, 320, 321, 350, 354, 355, 356, 357

1ng3A Complex of thio (glycine oxidase) with acetyl-glycine (see paper)
21% identity, 78% coverage: 54:393/434 of query aligns to 22:349/364 of 1ng3A

query
sites
1ng3A

H

Y

L

L

A

A

E

K

A

E

G

N

F

K

D

N

V

T

V

A

L

L

L
x
F

E
|
E

A
x
S

H

G

R

T

A

M

G

G

F

G

G
x
R

A
x
T

S
x
T

G

S

R

A

N
x
A

G
x
A

G
|
G

Q
x
M

L

L

G

G

S

A

A

H

Q

A

R

E

M

C

D

E

Q

E

E

R

D

D

L

-

E

-

R

-

L

-

V

-

G

-

D

-

A

-

L

-

K

A

L

F

W

F

D

D

L

F

A

A

E

M

D

H

A

S

K

Q

D

R

L

L

V

Y

K

K

S

G

L

L

-

G

-

E

-

L

-

Y

-

A

-

L

-

S

-

G

-

V

-

D

I

I

S
x
R

K

Q

H

H

Q

N

I

-

D

-

C

-

D

-

L

-

K

-

P

G

G

G

I

M

A

F

V

K

L

L

G

A

F

F

N

S

D

E

S

E

E

D

R

V

A

L

E

Q

L

L

H

R

D

-

H

-

A

-

H

-

L

-

S

-

G

-

R

-

Y

-

K

Q

Y

M

D

D

Q

D

I

L

E

D

A

S

L

V

D

S

A

W

D

Y

A

S

A

K

R

E

G

E

L

V

C

L

P

E

S

K

P

E

A

P

Y

Y

A

A

G

S

G

G

Y

D

L

I

D

F

N

G

G

A

A

S

-

F

-

I

-

Q

-

D

A

V

H

H

L

V

H

E

P

P

L

Y

N

F

Y

V

A

C

L

K

G

A

L

Y

A

V

R

K

A

A

V

K

G

M

A

L

G

G

L

A

R

E

L

I

H

F

E

E

D

H

T

T

E

P

V
|
V

L

L

G

H

I

V

D

E

Q

R

-

D

G

G

A

E

R

A

V

L

T

F

V

I

R

K

T

T

E

P

K

S

G

G

K

D

I

V

T

W

A

A

D

N

N

H

V

V

I

V

L

V

A

A

C
x
S

N
x
G

G

V

Y
x
W

L

S

G

G

D

M

L
x
F

N

F

R

K

Q

Q

V

L

A

G

S

-

R

-

V

-

M

-

P

-

I

L

N

N

F

-

I

-

A

-

A

A

T

F

E

L

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P

L

V

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K

A

G

E

E

A

C

Q

L

K

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V

L

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A

N

K

D

D

D

I

I

A

P

V

L

A

T

D

K

T

T

K

L

F

Y

V

H

V

D

N

H

-

C

Y
|
Y

F

I

R

V

L

P

S

R

A

K

D

S

G

G

R
|
R

L

L

L

V

F

V

G

G

G

A

G

T

E

M

S

K

Y

P

G

G

-

D

Y

W

R

S

F

E

P

T

E

P

D

D

I

L

A

G

-

L

-

E

K

S

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V

R

M

K

K

P

K

M

A

T

K

E

T

I

M

Y

L

P

P

H

P

L

I

R

Q

D

N

V

M

K

K

I

V

D

D

Y

R

A

F

W

W

G

A

G
|
G

T

L

L
x
R

G

P

I

G

T

T

M

K

R

D

R

G

M

K

P

P

Y

Y

L

I

A

G

R

R

I

H

A

P

P

E

N

D

-

S

-

R

I

I

L

L

S

F

A

A

S

A

G

G

Y
x
H

S

F

G
x
R

H
x
N

G
|
G

V
x
I

G

L

T

L

A

A

T

P

H

A

A

T

G

G

Q

A

L

L

M

I

A

S

L

D

A

L

I

I

Q

M

G

N

Q

K

active site: A47 (≠ G79), G48 (= G80), M49 (≠ Q81)
binding acetylamino-acetic acid: Y246 (= Y295), R302 (≠ L348), R329 (≠ G373)
binding flavin-adenine dinucleotide: F33 (≠ L65), E34 (= E66), S35 (≠ A67), R41 (≠ G73), T42 (≠ A74), T43 (≠ S75), A46 (≠ N78), A47 (≠ G79), G48 (= G80), M49 (≠ Q81), V174 (= V217), S202 (≠ C244), G203 (≠ N245), W205 (≠ Y247), F209 (≠ L251), G300 (= G346), R302 (≠ L348), H327 (≠ Y371), R329 (≠ G373), N330 (≠ H374), G331 (= G375), I332 (≠ V376)
binding phosphate ion: R89 (≠ S121), R254 (= R303)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 11, 13, 15

O31616 Glycine oxidase; GO; EC 1.4.3.19 from Bacillus subtilis (strain 168) (see 3 papers)
21% identity, 78% coverage: 54:393/434 of query aligns to 22:349/369 of O31616

query
sites
O31616

H

Y

L

L

A

A

E

K

A

E

G

N

F

K

D

N

V

T

V

A

L

L

L

F

E
|
E

A
x
S

H

G

R

T

A

M

G

G

F

G

G

R

A
x
T

S
x
T

G

S

R

A

N

A

G
x
A

G
|
G

Q
x
M

L

L

G
|
G

S

A

A

H

Q
x
A

R

E

M

C

D

E

Q

E

E

R

D

D

L

-

E

-

R

-

L

-

V

-

G

-

D

-

A

-

L

-

K

A

L

F

W

F

D

D

L

F

A

A

E

M

D

H

A

S

K

Q

D

R

L

L

V

Y

K

K

S

G

L

L

-

G

-

E

-

L

-

Y

-

A

-

L

-

S

-

G

-

V

-

D

I

I

S

R

K

Q

H

H

Q

N

I

-

D

-

C

-

D

-

L

-

K

-

P

G

G

G

I

M

A

F

V

K

L

L

G

A

F

F

N

S

D

E

S

E

E

D

R

V

A

L

E

Q

L

L

H

R

D

-

H

-

A

-

H

-

L

-

S

-

G

-

R

-

Y

-

K

Q

Y

M

D

D

Q

D

I

L

E

D

A

S

L

V

D

S

A

W

D

Y

A

S

A

K

R

E

G

E

L

V

C

L

P

E

S

K

P

E

A

P

Y

Y

A

A

G

S

G

G

Y

D

L

I

D

F

N

G

G

A

A

S

-

F

-

I

-

Q

-

D

A

V

H

H

L

V

H

E

P

P

L

Y

N

F

Y

V

A

C

L

K

G

A

L

Y

A

V

R

K

A

A

V

K

G

M

A

L

G

G

L

A

R

E

L

I

H

F

E

E

D

H

T

T

E

P

V
|
V

L

L

G

H

I

V

D

E

Q

R

-

D

G

G

A

E

R

A

V

L

T

F

V

I

R

K

T

T

E

P

K

S

G

G

K

D

I

V

T

W

A

A

D

N

N

H

V

V

I

V

L

V

A

A

C

S

N

G

G

V

Y

W

L

S

G

G

D

M

L

F

N

F

R

K

Q

Q

V

L

A

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-

R

-

V

-

M

-

P

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L

N

N

F

-

I

-

A

-

A

A

T

F

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L

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L

V

G

K

A

G

E

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C

Q

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S

V

V

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W

A

N

K

D

D

D

I

I

A

P

V

L

A

T

D

K

T

T

K

L

F

Y

V

H

V

D

N
x
H

-

C

Y

Y

F

I

R

V

L

P

S

R

A

K

D

S

G

G

R

R

L

L

L

V

F

V

G

G

G

A

G

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M

S

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Y

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G

G

-

D

Y

W

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D

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L

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-

L

-

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S

V

V

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K

P

K

M

A

T

K

E

T

I

M

Y

L

P

P

H

A

L

I

R

Q

D

N

V

M

K

K

I

V

D

D

Y

R

A

F

W

W

G

A

G

G

T

L

L
x
R

G

P

I

G

T

T

M

K

R

D

R

G

M

K

P

P

Y

Y

L

I

A

G

R

R

I

H

A

P

P

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N

D

-

S

-

R

I

I

L

L

S

F

A

A

S

A

G

G

Y
x
H

S
x
F

G
x
R

H
x
N

G
|
G

V
x
I

G
x
L

T

L

A

A

T

P

H

A

A

T

G

G

Q

A

L

L

M

I

A

S

L

D

A

L

I

I

Q

M

G

N

Q

K

ES 34:35 (≠ EA 66:67) binding FAD
TT 42:43 (≠ AS 74:75) binding FAD
AGM 47:49 (≠ GGQ 79:81) binding FAD
G51 (= G83) mutation to R: 130-fold decrease in catalytic efficiency on glycine and 28-fold increase in that on glyphosate.; mutation to S: 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with R-54 and A-244.
A54 (≠ Q86) mutation to R: 20-fold decrease in catalytic efficiency on glycine and 34-fold increase in that on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and A-244.
V174 (= V217) binding FAD
H244 (≠ N294) mutation to A: 2-fold decrease in catalytic efficiency on glycine and similar catalytic efficiency on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and R-54.
R302 (≠ L348) binding substrate
327:333 (vs. 371:377, 14% identical) binding FAD
R329 (≠ G373) binding substrate

Sites not aligning to the query:

14:15 binding FAD

Query Sequence

>WP_010439151.1 NCBI__GCF_000192475.1:WP_010439151.1
MNLLYSNDRKAEYPPSWYAATAHPFDRYPALVGDHTADVCIVGGGYTGLSAALHLAEAGF
DVVLLEAHRAGFGASGRNGGQLGSAQRMDQEDLERLVGDDDALKLWDLAEDAKDLVKSLI
SKHQIDCDLKPGIAVLGFNDSERAELHDHAAHLSGRYKYDQIEALDADAARGLCPSPAYA
GGYLDNGAAHLHPLNYALGLARAAVGAGLRLHEDTEVLGIDQGARVTVRTEKGKITADNV
ILACNGYLGDLNRQVASRVMPINNFIAATEPLGAEAQKVLAKDIAVADTKFVVNYFRLSA
DGRLLFGGGESYGYRFPEDIAAKVRKPMTEIYPHLRDVKIDYAWGGTLGITMRRMPYLAR
IAPNILSASGYSGHGVGTATHAGQLMALAIQGQAEGFDTMARVPALPFPGGPALRTPLLI
LAMTWYATRDRLGI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory