SitesBLAST

Comparing WP_010440013.1 NCBI__GCF_000192475.1:WP_010440013.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Found 20 (the maximum) hits to proteins with known functional sites

5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
34% identity, 98% coverage: 4:260/261 of query aligns to 6:257/259 of 5zaiC

• active site: A65 (≠ H63), F70 (≠ M68), S82 (= S80), R86 (≠ A90), G110 (≠ A114), E113 (≠ Q117), P132 (≠ N136), E133 (≠ G137), I138 (≠ L142), P140 (≠ C144), G141 (≠ S145), A226 (= A229), F236 (≠ M239)
• binding coenzyme a: K24 (≠ R22), L25 (= L23), A63 (= A61), G64 (= G62), A65 (≠ H63), D66 (= D64), I67 (≠ L65), P132 (≠ N136), R166 (≠ F169), F248 (= F251), K251 (= K254)

6slbAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
35% identity, 100% coverage: 1:260/261 of query aligns to 4:255/257 of 6slbAAA

• active site: Q64 (≠ H63), F69 (≠ M68), L80 (= L85), N84 (≠ A90), A108 (= A114), S111 (≠ Q117), A130 (≠ N136), F131 (≠ G137), L136 (= L142), P138 (= P147), D139 (≠ M148), A224 (= A229), G234 (≠ M239)
• binding (~{E})-6-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-6-oxidanylidene-hex-3-enoic acid: R58 (≠ K57), A62 (= A61), Q64 (≠ H63), D65 (= D64), L66 (= L65), Y76 (= Y81), A108 (= A114), F131 (≠ G137), D139 (≠ M148)

6slaAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
34% identity, 100% coverage: 1:260/261 of query aligns to 1:243/245 of 6slaAAA

• active site: Q61 (≠ H63), L68 (≠ F86), N72 (≠ A90), A96 (= A114), S99 (≠ Q117), A118 (≠ N136), F119 (≠ G137), L124 (= L142), P126 (= P147), N127 (≠ M148), A212 (= A229), G222 (≠ M239)
• binding ~{S}-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] 2-(2,5-dihydrooxepin-7-yl)ethanethioate: L21 (= L23), A59 (= A61), Q61 (≠ H63), D62 (= D64), L63 (= L65), L68 (≠ F86), Y71 (≠ C89), A94 (≠ T112), G95 (≠ A113), A96 (= A114), F119 (≠ G137), I122 (= I140), L124 (= L142), N127 (≠ M148), F234 (= F251), K237 (= K254)

O53561 Enoyl-CoA hydratase EchA19; EC 4.2.1.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
33% identity, 99% coverage: 2:260/261 of query aligns to 10:264/266 of O53561

• K135 (≠ R132) modified: N6-succinyllysine; mutation to E: Nearly wild-type levels of succinylation in vitro, reduces specific activity 8-fold.
• 135:142 (vs. 132:139, 25% identical) mutation to EFGISEAE: Very low levels of succinylation in vitro, reduces specific activity 15-fold.
• K142 (≠ N139) modified: N6-succinyllysine; mutation to E: About 50% succinylation in vitro, reduces specific activity 7-fold.

2dubA Enoyl-coa hydratase complexed with octanoyl-coa (see paper)
30% identity, 98% coverage: 5:259/261 of query aligns to 9:251/254 of 2dubA

• active site: A67 (≠ H63), M72 (= M68), S82 (≠ K91), G105 (≠ A114), E108 (≠ Q117), P127 (≠ N136), E128 (≠ G137), T133 (≠ L142), P135 (≠ C144), G136 (≠ S145), K221 (≠ A229), F231 (≠ M239)
• binding octanoyl-coenzyme a: K25 (≠ E21), A26 (≠ R22), L27 (= L23), A29 (= A25), A65 (= A61), A67 (≠ H63), D68 (= D64), I69 (≠ L65), K70 (= K66), G105 (≠ A114), E108 (≠ Q117), P127 (≠ N136), E128 (≠ G137), G136 (≠ S145), A137 (≠ T146)

2hw5C The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1
30% identity, 98% coverage: 5:260/261 of query aligns to 10:258/260 of 2hw5C

• active site: A68 (≠ H63), M73 (= M68), S83 (≠ D87), L87 (≠ A90), G111 (≠ A114), E114 (≠ Q117), P133 (≠ N136), E134 (≠ G137), T139 (≠ S145), P141 (= P147), G142 (≠ M148), K227 (≠ A229), F237 (≠ M239)
• binding crotonyl coenzyme a: K26 (≠ E21), A27 (≠ R22), L28 (= L23), A30 (= A25), K62 (= K57), I70 (≠ L65), F109 (≠ T112)

1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
30% identity, 98% coverage: 5:259/261 of query aligns to 10:255/258 of 1mj3A

• active site: A68 (≠ H63), M73 (= M68), S83 (≠ R77), L85 (= L79), G109 (≠ A114), E112 (≠ Q117), P131 (≠ N136), E132 (≠ G137), T137 (≠ L142), P139 (≠ C144), G140 (≠ S145), K225 (≠ A229), F235 (≠ M239)
• binding hexanoyl-coenzyme a: K26 (≠ E21), A27 (≠ R22), L28 (= L23), A30 (= A25), A66 (= A61), G67 (= G62), A68 (≠ H63), D69 (= D64), I70 (≠ L65), G109 (≠ A114), P131 (≠ N136), E132 (≠ G137), L135 (≠ I140), G140 (≠ S145)

1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
30% identity, 98% coverage: 5:259/261 of query aligns to 10:257/260 of 1dubA

• active site: A68 (≠ H63), M73 (= M68), S83 (≠ L79), L87 (≠ Q83), G111 (≠ A114), E114 (≠ Q117), P133 (≠ N136), E134 (≠ G137), T139 (≠ L142), P141 (≠ C144), G142 (≠ S145), K227 (≠ A229), F237 (≠ M239)
• binding acetoacetyl-coenzyme a: K26 (≠ E21), A27 (≠ R22), L28 (= L23), A30 (= A25), A66 (= A61), A68 (≠ H63), D69 (= D64), I70 (≠ L65), Y107 (≠ I110), G110 (≠ A113), G111 (≠ A114), E114 (≠ Q117), P133 (≠ N136), E134 (≠ G137), L137 (≠ I140), G142 (≠ S145), F233 (≠ M235), F249 (= F251)

P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
30% identity, 98% coverage: 5:259/261 of query aligns to 40:287/290 of P14604

• E144 (≠ Q117) mutation to D: Reduces activity 50-fold.; mutation to Q: Reduces activity 3300-fold.
• E164 (≠ G137) mutation to D: Reduces activity 1250-fold.; mutation to Q: Reduces activity 330000-fold.

Sites not aligning to the query:

• 1:29 modified: transit peptide, Mitochondrion

1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
30% identity, 98% coverage: 5:259/261 of query aligns to 8:255/258 of 1ey3A

• active site: A66 (≠ H63), M71 (= M68), S81 (≠ L79), L85 (≠ Q83), G109 (≠ A114), E112 (≠ Q117), P131 (≠ N136), E132 (≠ G137), T137 (≠ L142), P139 (≠ C144), G140 (≠ S145), K225 (≠ A229), F235 (≠ M239)
• binding 4-(n,n-dimethylamino)cinnamoyl-coa: K24 (≠ E21), L26 (= L23), A28 (= A25), A64 (= A61), G65 (= G62), A66 (≠ H63), D67 (= D64), I68 (≠ L65), L85 (≠ Q83), W88 (≠ F86), G109 (≠ A114), P131 (≠ N136), L135 (≠ I140), G140 (≠ S145)

Q8GYN9 1,4-dihydroxy-2-naphthoyl-CoA synthase, peroxisomal; DHNS; Enoyl-CoA hydratase/isomerase D; ECHID; Naphthoate synthase; EC 4.1.3.36 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
31% identity, 99% coverage: 3:261/261 of query aligns to 78:332/337 of Q8GYN9

Sites not aligning to the query:

• 20 H→V: Loss of peroxisomal targeting.

3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
29% identity, 98% coverage: 3:259/261 of query aligns to 7:254/255 of 3q0jC

• active site: A65 (≠ H63), M70 (= M68), T80 (≠ F82), F84 (= F86), G108 (≠ A114), E111 (≠ Q117), P130 (≠ N136), E131 (≠ G137), V136 (≠ L142), P138 (= P147), G139 (≠ M148), L224 (≠ A229), F234 (≠ M239)
• binding acetoacetyl-coenzyme a: Q23 (≠ E21), A24 (≠ R22), L25 (= L23), A27 (= A25), A63 (= A61), G64 (= G62), A65 (≠ H63), D66 (= D64), I67 (≠ L65), K68 (= K66), M70 (= M68), F84 (= F86), G107 (≠ A113), G108 (≠ A114), E111 (≠ Q117), P130 (≠ N136), E131 (≠ G137), P138 (= P147), G139 (≠ M148), M140 (≠ V149)

3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
29% identity, 98% coverage: 3:259/261 of query aligns to 7:254/255 of 3q0gC

• active site: A65 (≠ H63), M70 (= M68), T80 (≠ F82), F84 (= F86), G108 (≠ A114), E111 (≠ Q117), P130 (≠ N136), E131 (≠ G137), V136 (≠ L142), P138 (= P147), G139 (≠ M148), L224 (≠ A229), F234 (≠ M239)
• binding coenzyme a: L25 (= L23), A63 (= A61), I67 (≠ L65), K68 (= K66), Y104 (≠ I110), P130 (≠ N136), E131 (≠ G137), L134 (≠ I140)

3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
29% identity, 98% coverage: 3:259/261 of query aligns to 6:253/256 of 3h81A

• active site: A64 (≠ H63), M69 (= M68), T79 (≠ F82), F83 (= F86), G107 (≠ A114), E110 (≠ Q117), P129 (≠ N136), E130 (≠ G137), V135 (≠ L142), P137 (= P147), G138 (≠ M148), L223 (≠ A229), F233 (≠ M239)
• binding calcium ion: F233 (≠ M239), Q238 (≠ T244)

3q0gD Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
29% identity, 98% coverage: 3:259/261 of query aligns to 6:249/250 of 3q0gD

• active site: A64 (≠ H63), M69 (= M68), T75 (≠ F82), F79 (= F86), G103 (≠ A114), E106 (≠ Q117), P125 (≠ N136), E126 (≠ G137), V131 (≠ L142), P133 (= P147), G134 (≠ M148), L219 (≠ A229), F229 (≠ M239)
• binding Butyryl Coenzyme A: F225 (≠ M235), F241 (= F251)

4i52A Scmenb im complex with 1-hydroxy-2-naphthoyl-coa (see paper)
32% identity, 97% coverage: 10:261/261 of query aligns to 18:270/275 of 4i52A