SitesBLAST
Comparing WP_010441027.1 NCBI__GCF_000192475.1:WP_010441027.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P51174 Long-chain specific acyl-CoA dehydrogenase, mitochondrial; LCAD; EC 1.3.8.8 from Mus musculus (Mouse) (see paper)
50% identity, 97% coverage: 12:383/385 of query aligns to 54:426/430 of P51174
- K318 (= K275) modified: N6-acetyllysine; mutation to R: Reduces activity by 37%; reduces activity by 80% when associated with R-322.
- K322 (≠ Q279) modified: N6-acetyllysine; alternate; mutation to R: Reduces activity by 23%; reduces activity by 80% when associated with R-318.
Sites not aligning to the query:
- 42 modified: N6-acetyllysine; K→R: Reduces activity by 90% when associated with R-318 and R-322.
8w0uA Human lcad complexed with acetoacetyl coenzyme a (see paper)
49% identity, 97% coverage: 12:383/385 of query aligns to 22:394/398 of 8w0uA
- binding acetoacetyl-coenzyme a: M140 (= M129), S147 (= S136), Q150 (= Q139), S193 (vs. gap), H196 (≠ K183), Y250 (= Y239), E259 (= E248), R260 (= R249), Y379 (= Y368), G380 (= G369), G381 (= G370), I385 (≠ V374), L389 (= L378), R392 (= R381)
- binding flavin-adenine dinucleotide: I138 (≠ L127), M140 (= M129), T141 (= T130), G146 (= G135), S147 (= S136), F171 (= F160), S173 (≠ T162), R285 (= R274), F288 (= F277), L295 (≠ F284), Q353 (= Q342), L354 (= L343), G357 (= G346), V375 (= V364), Y379 (= Y368), T382 (= T371), E384 (= E373)
8w0tA Human lcad (see paper)
49% identity, 97% coverage: 12:383/385 of query aligns to 22:394/398 of 8w0tA
- binding flavin-adenine dinucleotide: I138 (≠ L127), M140 (= M129), T141 (= T130), G146 (= G135), S147 (= S136), F171 (= F160), I172 (= I161), S173 (≠ T162), R285 (= R274), F288 (= F277), L295 (≠ F284), Q353 (= Q342), L354 (= L343), G356 (= G345), G357 (= G346), V375 (= V364), T382 (= T371), E384 (= E373), I385 (≠ V374)
P28330 Long-chain specific acyl-CoA dehydrogenase, mitochondrial; LCAD; EC 1.3.8.8 from Homo sapiens (Human) (see 2 papers)
49% identity, 97% coverage: 12:383/385 of query aligns to 54:426/430 of P28330
- E291 (= E248) active site, Proton acceptor; mutation to Q: Loss of long-chain-acyl-CoA dehydrogenase activity. No effect on protein abundance. No effect on solubility. No effect on substrate binding.
- S303 (≠ I260) to T: in dbSNP:rs1801204
- K333 (= K290) to Q: in dbSNP:rs2286963
2pg0A Crystal structure of acyl-coa dehydrogenase from geobacillus kaustophilus
46% identity, 98% coverage: 7:384/385 of query aligns to 1:379/380 of 2pg0A
- active site: M124 (= M129), T125 (= T130), E243 (= E248), A364 (≠ G369), R376 (= R381)
- binding flavin-adenine dinucleotide: I122 (≠ L127), M124 (= M129), T125 (= T130), G130 (= G135), S131 (= S136), F155 (= F160), I156 (= I161), T157 (= T162), R269 (= R274), F272 (= F277), F279 (= F284), Q337 (= Q342), L338 (= L343), G340 (= G345), G341 (= G346), V359 (= V364), I362 (= I367), Y363 (= Y368), T366 (= T371), E368 (= E373), M369 (≠ V374)
8sgrA Human liver mitochondrial isovaleryl-coa dehydrogenase (see paper)
36% identity, 97% coverage: 11:385/385 of query aligns to 12:390/393 of 8sgrA
- binding flavin-adenine dinucleotide: S135 (≠ T130), G140 (= G135), S141 (= S136), W165 (≠ F160), T167 (= T162), R279 (= R274), F282 (= F277), I286 (≠ V281), F289 (= F284), Q347 (= Q342), C348 (≠ L343), G351 (= G346), L369 (≠ V364), G375 (= G370), T376 (= T371), L382 (≠ E377)
1ivhA Structure of human isovaleryl-coa dehydrogenase at 2.6 angstroms resolution: structural basis for substrate specificity (see paper)
36% identity, 97% coverage: 11:385/385 of query aligns to 8:386/387 of 1ivhA
- active site: M130 (= M129), S131 (≠ T130), E249 (= E248), A370 (≠ G369), R382 (= R381)
- binding coenzyme a persulfide: S137 (= S136), S185 (≠ A182), R186 (≠ K183), V239 (≠ F238), Y240 (= Y239), M243 (= M242), E249 (= E248), R250 (= R249), G369 (≠ Y368), A370 (≠ G369), G371 (= G370), V375 (= V374)
- binding flavin-adenine dinucleotide: L128 (= L127), M130 (= M129), S131 (≠ T130), G136 (= G135), S137 (= S136), W161 (≠ F160), T163 (= T162), R275 (= R274), F278 (= F277), F285 (= F284), M288 (≠ T287), Q343 (= Q342), C344 (≠ L343), G347 (= G346), T372 (= T371), E374 (= E373)
P26440 Isovaleryl-CoA dehydrogenase, mitochondrial; IVD; Butyryl-CoA dehydrogenase; EC 1.3.8.4; EC 1.3.8.1 from Homo sapiens (Human) (see 5 papers)
36% identity, 97% coverage: 11:385/385 of query aligns to 45:423/426 of P26440
- 165:174 (vs. 127:136, 70% identical) binding FAD
- S174 (= S136) binding substrate
- WIT 198:200 (≠ FIT 160:162) binding FAD
- SR 222:223 (≠ AK 182:183) binding substrate
- G250 (= G211) to A: in IVA; uncertain significance
- Y277 (= Y239) binding substrate
- DLER 284:287 (≠ PWER 246:249) binding substrate
- E286 (= E248) active site, Proton acceptor; mutation to D: Residual isovaleryl-CoA dehydrogenase activity.; mutation to G: Loss of isovaleryl-CoA dehydrogenase activity. Does not affect isovaleryl-CoA dehydrogenase activity; when associated with 407-E.; mutation to Q: Loss of isovaleryl-CoA dehydrogenase activity.
- A291 (≠ G253) to V: in IVA; uncertain significance; dbSNP:rs886042098
- R312 (= R274) binding FAD
- Q323 (= Q285) binding FAD
- I379 (≠ L341) to T: in IVA; uncertain significance
- QCFGG 380:384 (≠ QLFGG 342:346) binding FAD
- R398 (≠ A360) to Q: in IVA; uncertain significance; dbSNP:rs1477527791
- Y403 (≠ Q365) to N: in IVA; uncertain significance
- A407 (≠ G369) mutation to E: Does not affect isovaleryl-CoA dehydrogenase activity; when associated with 286-D.
- AG 407:408 (≠ GG 369:370) binding substrate
- TSE 409:411 (≠ TNE 371:373) binding FAD
Sites not aligning to the query:
- 1:32 modified: transit peptide, Mitochondrion
4l1fA Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
37% identity, 97% coverage: 11:383/385 of query aligns to 5:377/380 of 4l1fA
- active site: L125 (≠ M129), T126 (= T130), G242 (≠ E248), E363 (≠ G369), R375 (= R381)
- binding coenzyme a persulfide: T132 (≠ S136), H179 (≠ K183), F232 (= F238), M236 (= M242), E237 (≠ K243), L239 (= L245), D240 (≠ P246), R243 (= R249), Y362 (= Y368), E363 (≠ G369), G364 (= G370), R375 (= R381)
- binding flavin-adenine dinucleotide: F123 (≠ L127), L125 (≠ M129), T126 (= T130), G131 (= G135), T132 (≠ S136), F156 (= F160), I157 (= I161), T158 (= T162), R268 (= R274), Q270 (≠ A276), F271 (= F277), I275 (≠ V281), F278 (= F284), L281 (≠ T287), Q336 (= Q342), I337 (≠ L343), G340 (= G346), I358 (≠ V364), Y362 (= Y368), T365 (= T371), Q367 (≠ E373)
- binding 1,3-propandiol: L5 (≠ V11), Q10 (≠ R16)
5ol2F The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
38% identity, 97% coverage: 12:383/385 of query aligns to 5:376/378 of 5ol2F
- active site: L124 (≠ M129), T125 (= T130), G241 (≠ E248), G374 (≠ R381)
- binding calcium ion: E29 (≠ K36), E33 (≠ D40), R35 (≠ V42)
- binding coenzyme a persulfide: L238 (= L245), R242 (= R249), E362 (≠ G369), G363 (= G370)
- binding flavin-adenine dinucleotide: F122 (≠ L127), L124 (≠ M129), T125 (= T130), P127 (= P132), T131 (≠ S136), F155 (= F160), I156 (= I161), T157 (= T162), E198 (≠ L204), R267 (= R274), F270 (= F277), L274 (≠ V281), F277 (= F284), Q335 (= Q342), L336 (= L343), G338 (= G345), G339 (= G346), Y361 (= Y368), T364 (= T371), E366 (= E373)
3r7kA Crystal structure of a probable acyl coa dehydrogenase from mycobacterium abscessus atcc 19977 / dsm 44196 (see paper)
36% identity, 97% coverage: 9:383/385 of query aligns to 3:377/378 of 3r7kA
- active site: V126 (≠ M129), T127 (= T130), E242 (= E248), G363 (= G369), K375 (≠ R381)
- binding dihydroflavine-adenine dinucleotide: V126 (≠ M129), T127 (= T130), G132 (= G135), S133 (= S136), F157 (= F160), I158 (= I161), T159 (= T162), R268 (= R274), T270 (≠ A276), F271 (= F277), L275 (≠ V281), R278 (≠ F284), I281 (≠ T287), Q336 (= Q342), I337 (≠ L343), G340 (= G346), I358 (≠ V364), T365 (= T371), E367 (= E373)
5lnxD Crystal structure of mmgc, an acyl-coa dehydrogenase from bacillus subtilis.
37% identity, 96% coverage: 14:383/385 of query aligns to 5:372/374 of 5lnxD