SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_010441641.1 NCBI__GCF_000192475.1:WP_010441641.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6ymfA Crystal structure of serine hydroxymethyltransferase from aphanothece halophytica in the plp-serine external aldimine state (see paper)
59% identity, 99% coverage: 6:417/417 of query aligns to 7:414/418 of 6ymfA

query
sites
6ymfA

L

L

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L

E

Q

A

T

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D

P

P

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T

I

I

A

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S

G

S

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K

E

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L

K

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R

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A

R

E

E

Q

H

I

L

E

E

L

L

I

I

A

A

S
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S

E

E

N

N

I

F

V

T

S

S

Q

P

A

A

V

V

I

M

D

A

A

T

Q

Q

G

G

S

S

V

V

L

L

T

T

N

N

K

K

Y
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Y

A

A

E
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E

G

G

Y

L

P

P

G

K

R

K

R

R

Y

Y

Y

Y

G

G

G

G

C

C

E

E

Y

F

V

I

D

D

E

E

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I

E

E

A

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E

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A

A

I

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E

D

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R

L

A

K

K

Q

E

L

L

F

F

G

G

C

A

E

A

Y

S

A

A

N

N

V

V

Q

Q

P

P

H

H

S
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S

G
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G

A
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A

Q

Q

A

A

N

N

G

F

A

A

V

V

K

F

L

L

A

T

L

L

L

L

S

K

P

P

G

G

D

D

T

K

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I

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G

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M

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L

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S

A

H

G

G

G

G

H
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H

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L

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T

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H

G

G

A

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K

P

P

A

A

N

Q

V

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S

G

G

K

K

W

W

F

F

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E

P

A

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V

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H

Y

Y

G

G

L

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T

S

-

Q

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E

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D

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Y

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Y

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K

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N

F

F

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E

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K

F

F

R

R

A

A

I

I

A

A

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D

E

E

V

V

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G

A

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W

Y

L

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D

M

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A
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H
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H

I

I

A

A

G

G

L

L

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V

A

A

T

S

G

G

L

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H

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N

P

P

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P

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H

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C

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D

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T

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K
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K

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R

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E

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H

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I

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A

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G

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K

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A

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G

E

E

A

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L

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F

K

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E

A

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I

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A

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A

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M

A

A

N

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M

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A

N

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R

G

G

C

F

D

K

I

I

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S

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G

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H

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K

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R

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F

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K

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Q

E

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G

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N

L

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I

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G

A

N

D

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R

L

L

D

-

A

-

L

-

A

-

Q

Q

S

N

P

P

D

E

G

-

D

D

E

E

A

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K

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K

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A

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P

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L

Y

Y

active site: Y54 (= Y53), E56 (= E55), D200 (= D198), T226 (= T224), K229 (= K227), R235 (= R233)
binding [3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-serine: S34 (= S33), S96 (= S95), G97 (= G96), A98 (= A97), H125 (= H124), S175 (= S173), D200 (= D198), A202 (= A200), H203 (= H201), T226 (= T224), K229 (= K227), R361 (= R359)

6ymdA Crystal structure of serine hydroxymethyltransferase from aphanothece halophytica in the covalent complex with malonate (see paper)
59% identity, 99% coverage: 6:417/417 of query aligns to 7:414/420 of 6ymdA

query
sites
6ymdA

L

L

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L

E

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A

T

D

D

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P

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I

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A

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S

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S

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K

E

E

G

L

K

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R

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Q

A

R

E

E

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H

I

L

E

E

L

L

I

I

A

A

S
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S

E

E

N

N

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F

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S

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A

A

V

V

I

M

D

A

A

T

Q

Q

G

G

S

S

V

V

L

L

T

T

N

N

K

K

Y
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Y

A

A

E
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E

G

G

Y

L

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P

G

K

R

K

R

R

Y

Y

Y
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Y

G

G

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G

C

C

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E

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D

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K

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S

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A

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N

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V

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S

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A
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A

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Q

A

A

N

N

G

F

A

A

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V

K

F

L

L

A

T

L

L

L

L

S

K

P

P

G

G

D

D

T

K

I

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M

G

G

M

M

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D

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L

D

S

A

H

G

G

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H
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H

L

L

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T

H

H

G

G

A

S

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P

P

A

A

N

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S

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G

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K

W

W

F

F

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E

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A

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V

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Y

Y

G

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L

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-

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E

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E

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D

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Y

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L

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I

I

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G

A
x
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S

A

A

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Y

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K

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D

N

F

F

A

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F

F

R

R

A

A

I

I

A

A

D

D

E

E

V

V

G

G

A

A

W

Y

L

L

L

L

A

A

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D

M

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A
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A

H
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H

I

I

A

A

G

G

L

L

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V

A

A

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S

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G

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P

P

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D

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T
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T

T

T

H

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K

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L

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R

G

G

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A

A

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A

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A

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R

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G

C

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D

K

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S

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G

G

G

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T

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D

N

N

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H

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M

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D

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R

L

L

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A

A

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M

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T

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R

G

G

F

L

G

G

S

T

E

E

E

D

F

F

K

A

Q

E

I

I

G

A

H

N

L

I

I

I

G

A

N

D

V

R

L

L

D

-

A

-

L

-

A

-

Q

Q

S

N

P

P

D

E

G

-

D

D

E

E

A

Q

V

V

E

K

K

Q

E

A

T

C

R

V

E

Q

K

R

V

V

R

A

A

A

L

L

C

C

A

E

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R

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F

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P

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L

Y

Y

active site: Y54 (= Y53), E56 (= E55), D200 (= D198), T226 (= T224), K229 (= K227), R235 (= R233)
binding malonate ion: S34 (= S33), Y54 (= Y53), E56 (= E55), Y64 (= Y63), H125 (= H124), H203 (= H201), K229 (= K227), R361 (= R359)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: Y54 (= Y53), S96 (= S95), G97 (= G96), A98 (= A97), H125 (= H124), Y174 (≠ A172), S175 (= S173), D200 (= D198), A202 (= A200), T226 (= T224), K229 (= K227), G261 (= G259)

1kl2A Crystal structure of serine hydroxymethyltransferase complexed with glycine and 5-formyl tetrahydrofolate (see paper)
61% identity, 98% coverage: 4:411/417 of query aligns to 2:404/405 of 1kl2A

query
sites
1kl2A

K

K

S

Y

L

L

I

P

E

Q

A

Q

D

D

P

P

V

Q

I

V

A

F

S

A

S

A

I

I

A

E

R

Q

E

E

G

R

K

K

R

R

Q

Q

A

H

E

A

Q

K

I

I

E

E

L

L

I

I

A

A

S
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S

E

E

N

N

I

F

V

V

S

S

Q

R

A

A

V

V

I

M

D

E

A

A

Q

Q

G

G

S

S

V

V

L

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

G

G

R

R

R

R

Y
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Y

Y
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Y

G

G

G

G

C

C

E

E

Y

Y

V

V

D

D

E

I

V

V

E

E

A

E

E

L

A

A

I

R

E

E

R

R

L

A

K

K

Q

Q

L

L

F

F

G

G

C

A

E

E

Y

H

A

A

N

N

V

V

Q

Q

P

P

H

H

S
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S

G
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G

A
|
A

Q

Q

A

A

N

N

G

M

A

A

V

V

K

Y

L

F

A

T

L

V

L

L

S

E

P

H

G

G

D

D

T

T

I

V

L

L

G

G

M

M

S

N

L

L

D

S

A

H

G

G

G
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G

H
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H

L

L

T

T

H

H

G

G

A

S

K

P

P

V

A

N

Q

F

S

S

G

G

K

V

W

Q

F

Y

R

N

P

F

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V

Q

A

Y

Y

G

G

L

V

T

D

-

P

E

E

A

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H

L

V

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I

D

D

Y

Y

D

D

Q

D

V

V

E

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A

E

L

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A

A

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R

A

L

E

H

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R

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P

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K

L

L

I

I

I

V

A

A

G

A

A

A

S
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S

A

A

Y

Y

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K

I

I

I

D

D

F

F

A

A

R

K

F

F

R

R

A

E

I

I

A

A

D

D

E

E

V

V

G

G

A

A

W

Y

L

L

L

M

A

V

D
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D

M

M

A
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A

H
|
H

I

I

A

A

G

G

L

L

V

V

A

A

T

A

G

G

L

L

H

H

P

P

S

N

P

P

V

V

G

P

H

Y

A

A

H

H

V

F

I

V

T

T

S

T

T
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T

T

T

H
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H

K
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K

T

T

L

L

R

R

G

G

P

P

R
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R

G

G

G

G

V

M

I

I

L

L

T

C

N

Q

D

-

E

E

A

Q

L

F

A

A

K

K

K

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M

I

N

D

S

K

A

A

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F

P

P

G

G

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Q

G

G

G

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P

P

L

L

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M

H

H

V

V

I

I

A

A

G

A

K

K

A

A

V

V

A

A

F

F

G

G

E

E

A

A

L

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K

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P

D

E

D

F

F

K

K

E

A

Y

Y

M

A

K

K

R

R

V

V

V

V

V

D

S

N

A

A

S

K

A

R

L

L

A

A

N

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V

A

M

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A

N

R

E

G

G

C

F

D

T

I

L

V

V

S

S

G

G

G

G

T

T

E

D

N

N

H

H

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M

L

L

L

V

V

D

D

L

L

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T

G

G

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K

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A

A

E

E

A

K

A

V

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L

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R

E

A

V

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G

F

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T

C

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N

N

K

K

N
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N

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T

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G

Y

D

D

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I

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S

G

G

I

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R

L

I

G

G

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A

A

A

A

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C

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S

T

R

R

G

G

F

F

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E

E

F

M

K

D

Q

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H

A

L

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I

I

G

G

N

L

V

V

L

L

D

K

A

N

L

V

A

-

Q

-

S

-

P

-

D

-

G

G

D

S

E

E

A

Q

V

A

E

L

K

E

E

E

T

A

R

R

E

Q

K

R

V

V

R

A

A

A

L

L

C

T

active site: Y51 (= Y53), E53 (= E55), D197 (= D198), T223 (= T224), K226 (= K227), R232 (= R233)
binding N-{[4-({[(6R)-2-amino-5-formyl-4-oxo-1,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)phenyl]carbonyl}-L-glutamic acid: E53 (= E55), Y60 (= Y62), G121 (= G123), H122 (= H124), S172 (= S173), F251 (= F253), N341 (= N343)
binding glycine: S31 (= S33), Y51 (= Y53), Y61 (= Y63), H200 (= H201), R357 (= R359)
binding pyridoxal-5'-phosphate: S93 (= S95), G94 (= G96), A95 (= A97), H122 (= H124), S172 (= S173), D197 (= D198), A199 (= A200), H200 (= H201), T223 (= T224), H225 (= H226), K226 (= K227)

1kl1A Crystal structure of serine hydroxymethyltransferase complexed with glycine (see paper)
61% identity, 98% coverage: 4:411/417 of query aligns to 2:404/405 of 1kl1A

query
sites
1kl1A

K

K

S

Y

L

L

I

P

E

Q

A

Q

D

D

P

P

V

Q

I

V

A

F

S

A

S

A

I

I

A

E

R

Q

E

E

G

R

K

K

R

R

Q

Q

A

H

E

A

Q

K

I

I

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

I

F

V

V

S

S

Q

R

A

A

V

V

I

M

D

E

A

A

Q

Q

G

G

S

S

V

V

L

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

G

G

R

R

R

R

Y

Y

Y

Y

G

G

G

G

C

C

E

E

Y

Y

V

V

D

D

E

I

V

V

E

E

A

E

E

L

A

A

I

R

E

E

R

R

L

A

K

K

Q

Q

L

L

F

F

G

G

C

A

E

E

Y

H

A

A

N

N

V

V

Q

Q

P

P

H

H

S
|
S

G
|
G

A
|
A

Q

Q

A

A

N

N

G

M

A

A

V

V

K

Y

L

F

A

T

L

V

L

L

S

E

P

H

G

G

D

D

T

T

I

V

L

L

G

G

M

M

S

N

L

L

D

S

A

H

G

G

G

G

H
|
H

L

L

T

T

H

H

G

G

A

S

K

P

P

V

A

N

Q

F

S

S

G

G

K

V

W

Q

F

Y

R

N

P

F

V

V

Q

A

Y

Y

G

G

L

V

T

D

-

P

E

E

A

T

G

H

L

V

I

I

D

D

Y

Y

D

D

Q

D

V

V

E

R

A

E

L

K

A

A

K

R

A

L

E

H

K

R

P

P

Q

K

L

L

I

I

I

V

A

A

G

A

A
|
A

S
|
S

A

A

Y

Y

S

P

Q

R

K

I

I

I

D

D

F

F

A

A

R

K

F

F

R

R

A

E

I

I

A

A

D

D

E

E

V

V

G

G

A

A

W

Y

L

L

L

M

A

V

D
|
D

M

M

A
|
A

H
|
H

I

I

A

A

G

G

L

L

V

V

A

A

T

A

G

G

L

L

H

H

P

P

S

N

P

P

V

V

G

P

H

Y

A

A

H

H

V

F

I

V

T

T

S

T

T
|
T

T

T

H
|
H

K
|
K

T

T

L

L

R

R

G

G

P

P

R
|
R

G

G

G

G

V

M

I

I

L

L

T

C

N

Q

D

-

E

E

A

Q

L

F

A

A

K

K

K

Q

M

I

N

D

S

K

A

A

V

I

F

F

P

P

G

G

L

I

Q

Q

G

G

G

G

P

P

L

L

M

M

H

H

V

V

I

I

A

A

G

A

K

K

A

A

V

V

A

A

F

F

G

G

E

E

A

A

L

L

K

Q

P

D

E

D

F

F

K

K

E

A

Y

Y

M

A

K

K

R

R

V

V

V

V

V

D

S

N

A

A

S

K

A

R

L

L

A

A

N

S

V

A

M

L

I

Q

A

N

R

E

G

G

C

F

D

T

I

L

V

V

S

S

G

G

G

G

T

T

E

D

N

N

H

H

L

L

M

L

L

L

V

V

D

D

L

L

R

R

P

P

I

Q

G

Q

V

L

T

T

G

G

K

K

D

T

A

A

E

E

A

K

A

V

L

L

E

D

R

E

A

V

G

G

F

I

T

T

C

V

N

N

K

K

N

N

S

T

V

I

P

P

G

Y

D

D

P

P

Q

E

K

S

P

P

T

F

I

V

T

T

S

S

G

G

I

I

R
|
R

L

I

G

G

T

T

A

A

A

A

G

V

C

T

S

T

R

R

G

G

F

F

G

G

S

L

E

E

E

E

F

M

K

D

Q

E

I

I

G

A

H

A

L

I

I

I

G

G

N

L

V

V

L

L

D

K

A

N

L

V

A

-

Q

-

S

-

P

-

D

-

G

G

D

S

E

E

A

Q

V

A

E

L

K

E

E

E

T

A

R

R

E

Q

K

R

V

V

R

A

A

A

L

L

C

T

active site: Y51 (= Y53), E53 (= E55), D197 (= D198), T223 (= T224), K226 (= K227), R232 (= R233)
binding glycine: S31 (= S33), H122 (= H124), R357 (= R359)
binding pyridoxal-5'-phosphate: S93 (= S95), G94 (= G96), A95 (= A97), H122 (= H124), A171 (= A172), S172 (= S173), D197 (= D198), A199 (= A200), H200 (= H201), T223 (= T224), H225 (= H226), K226 (= K227)

1kkpA Crystal structure of serine hydroxymethyltransferase complexed with serine (see paper)
61% identity, 98% coverage: 4:411/417 of query aligns to 2:404/405 of 1kkpA

query
sites
1kkpA

K

K

S

Y

L

L

I

P

E

Q

A

Q

D

D

P

P

V

Q

I

V

A

F

S

A

S

A

I

I

A

E

R

Q

E

E

G

R

K

K

R

R

Q

Q

A

H

E

A

Q

K

I

I

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

I

F

V

V

S

S

Q

R

A

A

V

V

I

M

D

E

A

A

Q

Q

G

G

S

S

V

V

L

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

G

G

R

R

R

R

Y

Y

Y

Y

G

G

G

G

C

C

E

E

Y

Y

V

V

D

D

E

I

V

V

E

E

A

E

E

L

A

A

I

R

E

E

R

R

L

A

K

K

Q

Q

L

L

F

F

G

G

C

A

E

E

Y

H

A

A

N

N

V

V

Q

Q

P

P

H

H

S
|
S

G
|
G

A
|
A

Q

Q

A

A

N

N

G

M

A

A

V

V

K

Y

L

F

A

T

L

V

L

L

S

E

P

H

G

G

D

D

T

T

I

V

L

L

G

G

M

M

S

N

L

L

D

S

A

H

G

G

G

G

H
|
H

L

L

T

T

H

H

G

G

A

S

K

P

P

V

A

N

Q

F

S

S

G

G

K

V

W

Q

F

Y

R

N

P

F

V

V

Q

A

Y

Y

G

G

L

V

T

D

-

P

E

E

A

T

G

H

L

V

I

I

D

D

Y

Y

D

D

Q

D

V

V

E

R

A

E

L

K

A

A

K

R

A

L

E

H

K

R

P

P

Q

K

L

L

I

I

I

V

A

A

G

A

A

A

S
|
S

A

A

Y

Y

S

P

Q

R

K

I

I

I

D

D

F

F

A

A

R

K

F

F

R

R

A

E

I

I

A

A

D

D

E

E

V

V

G

G

A

A

W

Y

L

L

L

M

A

V

D
|
D

M

M

A
|
A

H
|
H

I

I

A

A

G

G

L

L

V

V

A

A

T

A

G

G

L

L

H

H

P

P

S

N

P

P

V

V

G

P

H

Y

A

A

H

H

V

F

I

V

T

T

S

T

T
|
T

T

T

H

H

K
|
K

T

T

L

L

R

R

G

G

P

P

R
|
R

G

G

G

G

V

M

I

I

L

L

T

C

N

Q

D

-

E

E

A

Q

L

F

A

A

K

K

K

Q

M

I

N

D

S

K

A

A

V

I

F

F

P

P

G

G

L

I

Q

Q

G

G

G

G

P

P

L

L

M

M

H

H

V

V

I

I

A

A

G

A

K

K

A

A

V

V

A

A

F

F

G

G

E

E

A

A

L

L

K

Q

P

D

E

D

F

F

K

K

E

A

Y

Y

M

A

K

K

R

R

V

V

V

V

V

D

S

N

A

A

S

K

A

R

L

L

A

A

N

S

V

A

M

L

I

Q

A

N

R

E

G

G

C

F

D

T

I

L

V

V

S

S

G

G

G

G

T

T

E

D

N

N

H

H

L

L

M

L

L

L

V

V

D

D

L

L

R

R

P

P

I

Q

G

Q

V

L

T

T

G

G

K

K

D

T

A

A

E

E

A

K

A

V

L

L

E

D

R

E

A

V

G

G

F

I

T

T

C

V

N

N

K

K

N

N

S

T

V

I

P

P

G

Y

D

D

P

P

Q

E

K

S

P

P

T

F

I

V

T

T

S

S

G

G

I

I

R
|
R

L

I

G

G

T

T

A

A

A

A

G

V

C

T

S

T

R

R

G

G

F

F

G

G

S

L

E

E

E

E

F

M

K

D

Q

E

I

I

G

A

H

A

L

I

I

I

G

G

N

L

V

V

L

L

D

K

A

N

L

V

A

-

Q

-

S

-

P

-

D

-

G

G

D

S

E

E

A

Q

V

A

E

L

K

E

E

E

T

A

R

R

E

Q

K

R

V

V

R

A

A

A

L

L

C

T

active site: Y51 (= Y53), E53 (= E55), D197 (= D198), T223 (= T224), K226 (= K227), R232 (= R233)
binding pyridoxal-5'-phosphate: S93 (= S95), G94 (= G96), A95 (= A97), H122 (= H124), S172 (= S173), D197 (= D198), A199 (= A200), H200 (= H201), K226 (= K227)
binding serine: S31 (= S33), H122 (= H124), R357 (= R359)

1kkjA Crystal structure of serine hydroxymethyltransferase from b.Stearothermophilus (see paper)
61% identity, 98% coverage: 4:411/417 of query aligns to 2:404/405 of 1kkjA

query
sites
1kkjA

K

K

S

Y

L

L

I

P

E

Q

A

Q

D

D

P

P

V

Q

I

V

A

F

S

A

S

A

I

I

A

E

R

Q

E

E

G

R

K

K

R

R

Q

Q

A

H

E

A

Q

K

I

I

E

E

L

L

I

I

A

A

S

S

E

E

N

N

I

F

V

V

S

S

Q

R

A

A

V

V

I

M

D

E

A

A

Q

Q

G

G

S

S

V

V

L

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

G

G

R

R

R

R

Y

Y

Y

Y

G

G

G

G

C

C

E

E

Y

Y

V

V

D

D

E

I

V

V

E

E

A

E

E

L

A

A

I

R

E

E

R

R

L

A

K

K

Q

Q

L

L

F

F

G

G

C

A

E

E

Y

H

A

A

N

N

V

V

Q

Q

P

P

H

H

S
|
S

G
|
G

A
|
A

Q

Q

A

A

N

N

G

M

A

A

V

V

K

Y

L

F

A

T

L

V

L

L

S

E

P

H

G

G

D

D

T

T

I

V

L

L

G

G

M

M

S

N

L

L

D

S

A

H

G

G

G

G

H
|
H

L

L

T

T

H

H

G

G

A

S

K

P

P

V

A

N

Q

F

S

S

G

G

K

V

W

Q

F

Y

R

N

P

F

V

V

Q

A

Y

Y

G

G

L

V

T

D

-

P

E

E

A

T

G

H

L

V

I

I

D

D

Y

Y

D

D

Q

D

V

V

E

R

A

E

L

K

A

A

K

R

A

L

E

H

K

R

P

P

Q

K

L

L

I

I

I

V

A

A

G

A

A

A

S
|
S

A

A

Y

Y

S

P

Q

R

K

I

I

I

D

D

F

F

A

A

R

K

F

F

R

R

A

E

I

I

A

A

D

D

E

E

V

V

G

G

A

A

W

Y

L

L

L

M

A

V

D
|
D

M

M

A
|
A

H
|
H

I

I

A

A

G

G

L

L

V

V

A

A

T

A

G

G

L

L

H

H

P

P

S

N

P

P

V

V

G

P

H

Y

A

A

H

H

V

F

I

V

T

T

S

T

T
|
T

T

T

H
|
H

K
|
K

T

T

L

L

R

R

G

G

P

P

R
|
R

G

G

G

G

V

M

I

I

L

L

T

C

N

Q

D

-

E

E

A

Q

L

F

A

A

K

K

K

Q

M

I

N

D

S

K

A

A

V

I

F

F

P

P

G

G

L

I

Q

Q

G

G

G

G

P

P

L

L

M

M

H

H

V

V

I

I

A

A

G

A

K

K

A

A

V

V

A

A

F

F

G

G

E

E

A

A

L

L

K

Q

P

D

E

D

F

F

K

K

E

A

Y

Y

M

A

K

K

R

R

V

V

V

V

V

D

S

N

A

A

S

K

A

R

L

L

A

A

N

S

V

A

M

L

I

Q

A

N

R

E

G

G

C

F

D

T

I

L

V

V

S

S

G

G

G

G

T

T

E

D

N

N

H

H

L

L

M

L

L

L

V

V

D

D

L

L

R

R

P

P

I

Q

G

Q

V

L

T

T

G

G

K

K

D

T

A

A

E

E

A

K

A

V

L

L

E

D

R

E

A

V

G

G

F

I

T

T

C

V

N

N

K

K

N

N

S

T

V

I

P

P

G

Y

D

D

P

P

Q

E

K

S

P

P

T

F

I

V

T

T

S

S

G

G

I

I

R

R

L

I

G

G

T

T

A

A

A

A

G

V

C

T

S

T

R

R

G

G

F

F

G

G

S

L

E

E

E

E

F

M

K

D

Q

E

I

I

G

A

H

A

L

I

I

I

G

G

N

L

V

V

L

L

D

K

A

N

L

V

A

-

Q

-

S

-

P

-

D

-

G

G

D

S

E

E

A

Q

V

A

E

L

K

E

E

E

T

A

R

R

E

Q

K

R

V

V

R

A

A

A

L

L

C

T

active site: Y51 (= Y53), E53 (= E55), D197 (= D198), T223 (= T224), K226 (= K227), R232 (= R233)
binding pyridoxal-5'-phosphate: S93 (= S95), G94 (= G96), A95 (= A97), H122 (= H124), S172 (= S173), D197 (= D198), A199 (= A200), H200 (= H201), T223 (= T224), H225 (= H226), K226 (= K227)

2vmyA Crystal structure of f351gbsshmt in complex with gly and fthf (see paper)
61% identity, 98% coverage: 4:411/417 of query aligns to 2:404/405 of 2vmyA

query
sites
2vmyA

K

K

S

Y

L

L

I

P

E

Q

A

Q

D

D

P

P

V

Q

I

V

A

F

S

A

S

A

I

I

A

E

R

Q

E

E

G

R

K

K

R

R

Q

Q

A

H

E

A

Q

K

I

I

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

I

F

V

V

S

S

Q

R

A

A

V

V

I

M

D

E

A

A

Q

Q

G

G

S

S

V

V

L

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

G

G

R

R

R

R

Y
|
Y

Y
|
Y

G

G

G

G

C

C

E

E

Y

Y

V

V

D

D

E

I

V

V

E

E

A

E

E

L

A

A

I

R

E

E

R

R

L

A

K

K

Q

Q

L

L

F

F

G

G

C

A

E

E

Y

H

A

A

N

N

V

V

Q

Q

P

P

H

H

S
|
S

G
|
G

A
|
A

Q

Q

A

A

N

N

G

M

A

A

V

V

K

Y

L

F

A

T

L

V

L

L

S

E

P

H

G

G

D

D

T

T

I

V

L

L

G

G

M

M

S

N

L
|
L

D

S

A

H

G

G

G
|
G

H
|
H

L
|
L

T

T

H

H

G

G

A

S

K

P

P

V

A

N

Q

F

S

S

G

G

K

V

W

Q

F

Y

R

N

P

F

V

V

Q

A

Y

Y

G

G

L

V

T

D

-

P

E

E

A

T

G

H

L

V

I

I

D

D

Y

Y

D

D

Q

D

V

V

E

R

A

E

L

K

A

A

K

R

A

L

E

H

K

R

P

P

Q

K

L

L

I

I

I

V

A

A

G

A

A

A

S
|
S

A

A

Y

Y

S

P

Q

R

K

I

I

I

D

D

F

F

A

A

R

K

F

F

R

R

A

E

I

I

A

A

D

D

E

E

V

V

G

G

A

A

W

Y

L

L

L

M

A

V

D
|
D

M

M

A
|
A

H
|
H

I

I

A

A

G

G

L

L

V

V

A

A

T

A

G

G

L

L

H

H

P

P

S

N

P

P

V

V

G

P

H

Y

A

A

H

H

V

F

I

V

T

T

S

T

T
|
T

T

T

H

H

K
|
K

T

T

L

L

R

R

G

G

P

P

R
|
R

G

G

G

G

V

M

I

I

L

L

T

C

N

Q

D

-

E

E

A

Q

L

F

A

A

K

K

K

Q

M

I

N

D

S
x
K

A

A

V

I

F
|
F

P

P

G

G

L

I

Q

Q

G

G

G
|
G

P

P

L

L

M

M

H

H

V

V

I

I

A

A

G

A

K

K

A

A

V

V

A

A

F

F

G

G

E

E

A

A

L

L

K

Q

P

D

E

D

F

F

K

K

E

A

Y

Y

M

A

K

K

R

R

V

V

V

V

V

D

S

N

A

A

S

K

A

R

L

L

A

A

N

S

V

A

M

L

I

Q

A

N

R

E

G

G

C

F

D

T

I

L

V

V

S

S

G

G

G

G

T

T

E

D

N

N

H

H

L

L

M

L

L

L

V

V

D

D

L

L

R

R

P

P

I

Q

G

Q

V

L

T

T

G

G

K

K

D

T

A

A

E

E

A

K

A

V

L

L

E

D

R

E

A

V

G

G

F

I

T

T

C

V

N

N

K

K

N
|
N

S

T

V

I

P

P

G

Y

D

D

P

P

Q

E

K
x
S

P
|
P

T
x
G

I

V

T

T

S

S

G

G

I

I

R
|
R

L

I

G

G

T

T

A

A

A

A

G

V

C

T

S

T

R

R

G

G

F

F

G

G

S

L

E

E

E

E

F

M

K

D

Q

E

I

I

G

A

H

A

L

I

I

I

G

G

N

L

V

V

L

L

D

K

A

N

L

V

A

-

Q

-

S

-

P

-

D

-

G

G

D

S

E

E

A

Q

V

A

E

L

K

E

E

E

T

A

R

R

E

Q

K

R

V

V

R

A

A

A

L

L

C

T

active site: Y51 (= Y53), E53 (= E55), D197 (= D198), T223 (= T224), K226 (= K227), R232 (= R233)
binding N-[4-({[(6S)-2-amino-5-formyl-4-oxo-3,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)benzoyl]-L-glutamic acid: E53 (= E55), Y60 (= Y62), Y61 (= Y63), L117 (= L119), G121 (= G123), H122 (= H124), L123 (= L125), S172 (= S173), K248 (≠ S250), F251 (= F253), N341 (= N343), S349 (≠ K351), P350 (= P352), G351 (≠ T353), R357 (= R359)
binding glycine: S31 (= S33), Y51 (= Y53), Y61 (= Y63), H200 (= H201), K226 (= K227), R357 (= R359)
binding pyridoxal-5'-phosphate: Y51 (= Y53), S93 (= S95), G94 (= G96), A95 (= A97), H122 (= H124), S172 (= S173), D197 (= D198), A199 (= A200), H200 (= H201), T223 (= T224), K226 (= K227), G257 (= G259)

2vmxA Crystal structure of f351gbsshmt in complex with l-allo-thr (see paper)
61% identity, 98% coverage: 4:411/417 of query aligns to 2:404/405 of 2vmxA

query
sites
2vmxA

K

K

S

Y

L

L

I

P

E

Q

A

Q

D

D

P

P

V

Q

I

V

A

F

S

A

S

A

I

I

A

E

R

Q

E

E

G

R

K

K

R

R

Q

Q

A

H

E

A

Q

K

I

I

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

I

F

V

V

S

S

Q

R

A

A

V

V

I

M

D

E

A

A

Q

Q

G

G

S

S

V

V

L

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

G

G

R

R

R

R

Y

Y

Y

Y

G

G

G

G

C

C

E

E

Y

Y

V

V

D

D

E

I

V

V

E

E

A

E

E

L

A

A

I

R

E

E

R

R

L

A

K

K

Q

Q

L

L

F

F

G

G

C

A

E

E

Y

H

A

A

N

N

V

V

Q

Q

P

P

H

H

S
|
S

G
|
G

A
|
A

Q

Q

A

A

N

N

G

M

A

A

V

V

K

Y

L

F

A

T

L

V

L

L

S

E

P

H

G

G

D

D

T

T

I

V

L

L

G

G

M

M

S

N

L

L

D

S

A

H

G

G

G

G

H
|
H

L

L

T

T

H

H

G

G

A

S

K

P

P

V

A

N

Q

F

S

S

G

G

K

V

W

Q

F

Y

R

N

P

F

V

V

Q

A

Y

Y

G

G

L

V

T

D

-

P

E

E

A

T

G

H

L

V

I

I

D

D

Y

Y

D

D

Q

D

V

V

E

R

A

E

L

K

A

A

K

R

A

L

E

H

K

R

P

P

Q

K

L

L

I

I

I

V

A

A

G

A

A

A

S
|
S

A

A

Y

Y

S

P

Q

R

K

I

I

I

D

D

F

F

A

A

R

K

F

F

R

R

A

E

I

I

A

A

D

D

E

E

V

V

G

G

A

A

W

Y

L

L

L

M

A

V

D
|
D

M

M

A
|
A

H
|
H

I

I

A

A

G

G

L

L

V

V

A

A

T

A

G

G

L

L

H

H

P

P

S

N

P

P

V

V

G

P

H

Y

A

A

H

H

V

F

I

V

T

T

S

T

T
|
T

T

T

H

H

K
|
K

T

T

L

L

R

R

G

G

P

P

R
|
R

G

G

G

G

V

M

I

I

L

L

T

C

N

Q

D

-

E

E

A

Q

L

F

A

A

K

K

K

Q

M

I

N

D

S

K

A

A

V

I

F

F

P

P

G

G

L

I

Q

Q

G

G

G

G

P

P

L

L

M

M

H

H

V

V

I

I

A

A

G

A

K

K

A

A

V

V

A

A

F

F

G

G

E

E

A

A

L

L

K

Q

P

D

E

D

F

F

K

K

E

A

Y

Y

M

A

K

K

R

R

V

V

V

V

V

D

S

N

A

A

S

K

A

R

L

L

A

A

N

S

V

A

M

L

I

Q

A

N

R

E

G

G

C

F

D

T

I

L

V

V

S

S

G

G

G

G

T

T

E

D

N

N

H

H

L

L

M

L

L

L

V

V

D

D

L

L

R

R

P

P

I

Q

G

Q

V

L

T

T

G

G

K

K

D

T

A

A

E

E

A

K

A

V

L

L

E

D

R

E

A

V

G

G

F

I

T

T

C

V

N

N

K

K

N

N

S

T

V

I

P

P

G

Y

D

D

P

P

Q

E

K

S

P

P

T

G

I

V

T

T

S

S

G

G

I

I

R
|
R

L

I

G

G

T

T

A

A

A

A

G

V

C

T

S

T

R

R

G

G

F

F

G

G

S

L

E

E

E

E

F

M

K

D

Q

E

I

I

G

A

H

A

L

I

I

I

G

G

N

L

V

V

L

L

D

K

A

N

L

V

A

-

Q

-

S

-

P

-

D

-

G

G

D

S

E

E

A

Q

V

A

E

L

K

E

E

E

T

A

R

R

E

Q

K

R

V

V

R

A

A

A

L

L

C

T

active site: Y51 (= Y53), E53 (= E55), D197 (= D198), T223 (= T224), K226 (= K227), R232 (= R233)
binding allo-threonine: S31 (= S33), H122 (= H124), H200 (= H201), R357 (= R359)
binding pyridoxal-5'-phosphate: S93 (= S95), G94 (= G96), A95 (= A97), H122 (= H124), S172 (= S173), D197 (= D198), A199 (= A200), H200 (= H201), T223 (= T224), K226 (= K227)

4n0wA X-ray crystal structure of a serine hydroxymethyltransferase from burkholderia cenocepacia with covalently attached pyridoxal phosphate
59% identity, 100% coverage: 1:417/417 of query aligns to 2:415/416 of 4n0wA

query
sites
4n0wA

M

M

F

F

K

D

K

R

-

A

-

Q

-

S

S

T

L

I

I

A

E

N

A

V

D

D

P

P

V

E

I

I

A

F

S

A

S

A

I

I

A

E

R

Q

E

E

G

N

K

R

R

R

Q

Q

A

E

E

D

Q

H

I

I

E

E

L

L

I

I

A

A

S

S

E

E

N

N

I

Y

V

T

S

S

Q

P

A

A

V

V

I

M

D

A

A

A

Q

Q

G

G

S

S

V

Q

L

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

G

G

R

K

R

R

Y

Y

Y

Y

G

G

G

G

C

C

E

E

Y

Y

V

V

D

D

E

V

V

V

E

E

A

Q

E

L

A

A

I

I

E

D

R

R

L

V

K

K

Q

Q

L

L

F

F

G

G

C

A

E

E

Y

A

A

A

N

N

V

V

Q

Q

P

P

H

N

S
|
S

G
|
G

A
x
S

Q

Q

A

A

N

N

G

Q

A

G

V

V

K

F

L

F

A

A

L

M

L

L

S

K

P

P

G

G

D

D

T

T

I

I

L

M

G

G

M

M

S

S

L

L

D

A

A

H

G

G

G

G

H
|
H

L

L

T

T

H

H

G

G

A

S

K

P

P

V

A

N

Q

M

S

S

G

G

K

K

W

W

F

F

R

N

P

V

V

V

Q

S

Y

Y

G

G

L

L

T

N

E

E

A

N

G

E

L

D

I

I

D

D

Y

Y

D

D

Q

A

V

A

E

E

A

K

L

L

A

A

K

N

A

E

E

H

K

K

P

P

Q

K

L

L

I

I

I

V

A

A

G

G

A

A

S

S

A

A

Y

F

S

A

Q

L

K

K

I

I

D

D

F

F

A

E

R

R

F

L

R

A

A

K

I

I

A

A

D

K

E

S

V

V

G

G

A

A

W

Y

L

L

L

M

A

V

D
|
D

M

M

A
|
A

H
|
H

I

Y

A

A

G

G

L

L

V

I

A

A

T

A

G

G

L

V

H

Y

P

P

S

N

P

P

V

V

G

P

H

H

A

A

H

D

V

F

I

V

T

T

S

T

T
|
T

T

T

H

H

K
|
K

T

S

L

L

R

R

G

G

P

P

R
|
R

G

G

G

G

V

V

I

I

L

L

T

M

N

K

D

A

E

E

A

-

L

Y

A

E

K

K

K

P

M

I

N

N

S

S

A

A

V

I

F

F

P

P

G

G

L

I

Q

Q

G

G

G

G

P

P

L

L

M

M

H

H

V

V

I

I

A

A

G

A

K

K

A

A

V

V

A

A

F

F

G

K

E

E

A

A

L

L

K

S

P

P

E

E

F

F

K

K

E

E

Y

Y

M

Q

K

Q

R

K

V

V

V

V

V

E

S

N

A

A

S

R

A

V

L

L

A

A

N

E

V

T

M

L

I

V

A

K

R

R

G

G

C

L

D

R

I

I

V

V

S

S

G

G

G

R

T

T

E

E

N

S

H

H

L

V

M

M

L

L

V

V

D

D

L

L

R

R

P

A

I

K

G

H

V

I

T

T

G

G

K

K

D

A

A

A

E

E

A

A

A

A

L

L

E

G

R

A

A

A

G

H

F

I

T

T

C

V

N

N

K

K

N

N

S

A

V

I

P

P

G

N

D

D

P

P

Q

E

K

K

P

P

T

F

I

V

T

T

S

S

G

G

I

I

R

R

L

L

G

G

T

S

A

P

A

A

G

M

C

T

S

T

R

R

G

G

F

F

G

G

S

P

E

A

E

E

F

A

K

E

Q

Q

I

V

G

G

H

N

L

L

I

I

G

A

N

D

V

V

L

L

D

-

A

-

L

-

A

-

Q

E

S

N

P

P

D

E

G

-

D

D

E

A

A

A

V

T

E

I

K

E

E

R

T

V

R

R

E

A

K

Q

V

V

R

A

A

E

L

L

C

T

A

K

S

R

H

F

P

P

I

V

Y

Y

active site: Y57 (= Y53), E59 (= E55), D202 (= D198), T228 (= T224), K231 (= K227), R237 (= R233)
binding pyridoxal-5'-phosphate: S99 (= S95), G100 (= G96), S101 (≠ A97), H128 (= H124), D202 (= D198), A204 (= A200), H205 (= H201), K231 (= K227)

4ot8A X-ray crystal structure of serine hydroxymethyl transferase from burkholderia cenocepacia bound to plp and serine
59% identity, 100% coverage: 3:417/417 of query aligns to 5:413/414 of 4ot8A

query
sites
4ot8A

K

Q

K

S

S

T

L

I

I

A

E

N

A

V

D

D

P

P

V

E

I

I

A

F

S

A

S

A

I

I

A

E

R

Q

E

E

G

N

K

R

R

R

Q

Q

A

E

E

D

Q

H

I

I

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

I

Y

V

T

S

S

Q

P

A

A

V

V

I

M

D

A

A

A

Q

Q

G

G

S

S

V

Q

L

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

G

G

R

K

R

R

Y

Y

Y
|
Y

G

G

G

G

C

C

E

E

Y

Y

V

V

D

D

E

V

V

V

E

E

A

Q

E

L

A

A

I

I

E

D

R

R

L

V

K

K

Q

Q

L

L

F

F

G

G

C

A

E

E

Y

A

A

A

N

N

V

V

Q

Q

P

P

H

N

S
|
S

G
|
G

A
x
S

Q

Q

A

A

N

N

G

Q

A

G

V

V

K

F

L

F

A

A

L

M

L

L

S

K

P

P

G

G

D

D

T

T

I

I

L

M

G

G

M

M

S

S

L

L

D

A

A

H

G

G

G

G

H
|
H

L

L

T

T

H

H

G

G

A

S

K

P

P

V

A

N

Q

M

S

S

G

G

K

K

W

W

F

F

R

N

P

V

V

V

Q

S

Y

Y

G

G

L

L

T

N

E

E

A

N

G

E

L

D

I

I

D

D

Y

Y

D

D

Q

A

V

A

E

E

A

K

L

L

A

A

K

N

A

E

E

H

K

K

P

P

Q

K

L

L

I

I

I

V

A

A

G

G

A

A

S

S

A

A

Y

F

S

A

Q

L

K

K

I

I

D

D

F

F

A

E

R

R

F

L

R

A

A

K

I

I

A

A

D

K

E

S

V

V

G

G

A

A

W

Y

L

L

L

M

A

V

D
|
D

M

M

A
|
A

H
|
H

I

Y

A

A

G

G

L

L

V

I

A

A

T

A

G

G

L

V

H

Y

P

P

S

N

P

P

V

V

G

P

H

H

A

A

H

D

V

F

I

V

T

T

S

T

T
|
T

T

T

H

H

K
|
K

T

S

L

L

R

R

G

G

P

P

R
|
R

G

G

G

G

V

V

I

I

L

L

T

M

N

K

D

A

E

E

A

-

L

Y

A

E

K

K

K

P

M

I

N

N

S

S

A

A

V

I

F

F

P

P

G

G

L

I

Q

Q

G

G

G

G

P

P

L

L

M

M

H

H

V

V

I

I

A

A

G

A

K

K

A

A

V

V

A

A

F

F

G

K

E

E

A

A

L

L

K

S

P

P

E

E

F

F

K

K

E

E

Y

Y

M

Q

K

Q

R

K

V

V

V

V

V

E

S

N

A

A

S

R

A

V

L

L

A

A

N

E

V

T

M

L

I

V

A

K

R

R

G

G

C

L

D

R

I

I

V

V

S

S

G

G

G

R

T

T

E

E

N

S

H

H

L

V

M

M

L

L

V

V

D

D

L

L

R

R

P

A

I

K

G

H

V

I

T

T

G

G

K

K

D

A

A

A

E

E

A

A

A

A

L

L

E

G

R

A

A

A

G

H

F

I

T

T

C

V

N

N

K

K

N

N

S

A

V

I

P

P

G

N

D

D

P

P

Q

E

K

K

P

P

T

F

I

V

T

T

S

S

G

G

I

I

R
|
R

L

L

G

G

T

S

A

P

A

A

G

M

C

T

S

T

R

R

G

G

F

F

G

G

S

P

E

A

E

E

F

A

K

E

Q

Q

I

V

G

G

H

N

L

L

I

I

G

A

N

D

V

V

L

L

D

-

A

-

L

-

A

-

Q

E

S

N

P

P

D

E

G

-

D

D

E

A

A

A

V

T

E

I

K

E

E

R

T

V

R

R

E

A

K

Q

V

V

R

A

A

E

L

L

C

T

A

K

S

R

H

F

P

P

I

V

Y

Y

active site: Y55 (= Y53), E57 (= E55), D200 (= D198), T226 (= T224), K229 (= K227), R235 (= R233)
binding pyridoxal-5'-phosphate: S97 (= S95), G98 (= G96), S99 (≠ A97), H126 (= H124), D200 (= D198), A202 (= A200), H203 (= H201), K229 (= K227)
binding serine: S35 (= S33), E57 (= E55), Y65 (= Y63), H126 (= H124), H203 (= H201), R360 (= R359)

4otlA X-ray crystal structure of serine hydroxymethyl transferase from burkholderia cenocepacia bound to plp and glycine
60% identity, 99% coverage: 5:417/417 of query aligns to 2:408/409 of 4otlA

query
sites
4otlA

S

T

L

I

I

A

E

N

A

V

D

D

P

P

V

E

I

I

A

F

S

A

S

A

I

I

A

E

R

Q

E

E

G

N

K

R

R

R

Q

Q

A

E

E

D

Q

H

I

I

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

I

Y

V

T

S

S

Q

P

A

A

V

V

I

M

D

A

A

A

Q

Q

G

G

S

S

V

Q

L

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

G

G

R

K

R

R

Y

Y

Y
|
Y

G

G

G

G

C

C

E

E

Y

Y

V

V

D

D

E

V

V

V

E

E

A

Q

E

L

A

A

I

I

E

D

R

R

L

V

K

K

Q

Q

L

L

F

F

G

G

C

A

E

E

Y

A

A

A

N

N

V

V

Q

Q

P

P

H

N

S
|
S

G
|
G

A
x
S

Q

Q

A

A

N

N

G

Q

A

G

V

V

K

F

L

F

A

A

L

M

L

L

S

K

P

P

G

G

D

D

T

T

I

I

L

M

G

G

M

M

S

S

L

L

D

A

A

H

G

G

G

G

H
|
H

L

L

T

T

H

H

G

G

A

S

K

P

P

V

A

N

Q

M

S

S

G

G

K

K

W

W

F

F

R

N

P

V

V

V

Q

S

Y

Y

G

G

L

L

T

N

E

E

A

N

G

E

L

D

I

I

D

D

Y

Y

D

D

Q

A

V

A

E

E

A

K

L

L

A

A

K

N

A

E

E

H

K

K

P

P

Q

K

L

L

I

I

I

V

A

A

G

G

A

A

S
|
S

A

A

Y

F

S

A

Q

L

K

K

I

I

D

D

F

F

A

E

R

R

F

L

R

A

A

K

I

I

A

A

D

K

E

S

V

V

G

G

A

A

W

Y

L

L

L

M

A

V

D
|
D

M

M

A
|
A

H
|
H

I

Y

A

A

G

G

L

L

V

I

A

A

T

A

G

G

L

V

H

Y

P

P

S

N

P

P

V

V

G

P

H
&n