SitesBLAST
Comparing WP_010442059.1 NCBI__GCF_000192475.1:WP_010442059.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P12047 Adenylosuccinate lyase; ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor; EC 4.3.2.2 from Bacillus subtilis (strain 168) (see paper)
53% identity, 99% coverage: 1:431/434 of query aligns to 1:428/431 of P12047
- H89 (= H92) mutation to Q: Abolishes enzyme activity.
- H141 (= H144) mutation to Q: Abolishes enzyme activity.
- Q212 (= Q215) mutation to E: Decreases catalytic activity 1000-fold.; mutation to M: Abolishes enzyme activity.
- N270 (= N273) mutation N->D,L: Abolishes enzyme activity.
- R301 (= R304) mutation R->K,Q: Abolishes enzyme activity.
Q9X0I0 Adenylosuccinate lyase; ASL; Adenylosuccinase; ASase; EC 4.3.2.2 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
50% identity, 99% coverage: 1:430/434 of query aligns to 1:427/431 of Q9X0I0
- H141 (= H144) active site, Proton donor/acceptor
2x75A Staphylococcus aureus adenylosuccinate lyase (see paper)
48% identity, 99% coverage: 2:430/434 of query aligns to 1:423/427 of 2x75A
4eeiB Crystal structure of adenylosuccinate lyase from francisella tularensis complexed with amp and succinate
39% identity, 88% coverage: 1:383/434 of query aligns to 1:368/423 of 4eeiB
- active site: H67 (= H70), S140 (= S143), H141 (= H144), K256 (= K271), E263 (= E278)
- binding adenosine monophosphate: K66 (= K69), H67 (= H70), D68 (= D71), Q212 (= Q215), R289 (= R304), I291 (= I306), S294 (= S309), R298 (= R313)
5hw2A Crystal structure of adenylosuccinate lyase from francisella tularensis complexed with fumaric acid
39% identity, 88% coverage: 1:380/434 of query aligns to 1:365/419 of 5hw2A
5nx9D Crystal structure of neanderthal adenylosuccinate lyase (adsl) in complex with its products amp and fumarate (see paper)
26% identity, 87% coverage: 4:379/434 of query aligns to 13:397/477 of 5nx9D
- active site: H79 (= H70), T151 (≠ S143), H152 (= H144), S283 (= S266), K288 (= K271), E295 (= E278)
- binding 2-[9-(3,4-dihydroxy-5-phosphonooxymethyl-tetrahydro-furan-2-yl)-9h-purin-6-ylamino]-succinic acid: T151 (≠ S143), H152 (= H144)
- binding adenosine monophosphate: R13 (= R4), Y14 (= Y5), R78 (≠ K69), H79 (= H70), D80 (= D71), S105 (= S97), Q234 (= Q215), R296 (≠ N279), L324 (≠ I306), S327 (= S309), A328 (≠ S310), R331 (= R313)
- binding fumaric acid: H79 (= H70), S105 (= S97), Q234 (= Q215), S282 (= S265), S283 (= S266), K288 (= K271)
P30566 Adenylosuccinate lyase; ADSL; ASL; Adenylosuccinase; ASase; EC 4.3.2.2 from Homo sapiens (Human) (see 13 papers)
26% identity, 87% coverage: 4:379/434 of query aligns to 20:404/484 of P30566
- M26 (= M10) to L: in ADSLD; severe; dbSNP:rs1311171245
- I72 (≠ V54) to V: in ADSLD; severe
- P100 (≠ S85) to A: in ADSLD; moderate; dbSNP:rs119450942
- Y114 (≠ D99) to H: in ADSLD; severe; total loss of activity; dbSNP:rs374259530
- R141 (≠ A126) to W: in ADSLD; severe; dbSNP:rs756210458
- H159 (= H144) active site, Proton donor/acceptor
- R190 (= R175) to Q: in ADSLD; moderate; dbSNP:rs28941471
- R194 (≠ A179) to C: in ADSLD; severe; reduces protein stability; dbSNP:rs1465152683
- K246 (≠ D220) to E: in ADSLD; moderate; strongly reduced catalytic activity; dbSNP:rs119450944
- D268 (≠ E242) to N: in ADSLD; severe; total loss of activity; dbSNP:rs746501563
- S289 (= S265) active site, Proton donor/acceptor
- R303 (≠ N279) to C: in ADSLD; mild; strongly reduced activity with SAMP, but only slightly reduced activity with SAICAR; abolishes cooperativity; dbSNP:rs373458753
- L311 (≠ V287) to V: in ADSLD; severe; slightly reduced enzyme activity; dbSNP:rs2044791112
- P318 (≠ A294) to L: in ADSLD; severe; dbSNP:rs202064195
- V364 (≠ I339) to M: in ADSLD; severe; dbSNP:rs370851726
- R374 (≠ N349) to W: in ADSLD; severe; dbSNP:rs376533026
- S395 (= S370) to R: in ADSLD; severe
- R396 (= R371) to C: in ADSLD; severe; abolishes cooperativity and reduces enzyme activity; dbSNP:rs755492501; to H: in ADSLD; severe; abolishes cooperativity and reduces enzyme activity; dbSNP:rs763542069
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine; A → V: in ADSLD; severe; dbSNP:rs143083947
- 3 A → V: in ADSLD; severe
- 422 D → Y: in ADSLD; moderate; dbSNP:rs119450943
- 423 L → V: in ADSLD; moderate
- 426 R → H: in ADSLD; severe; most frequent mutation; dbSNP:rs119450941
- 430 D → N: in ADSLD; mild; dbSNP:rs554254383
- 438 S → P: in ADSLD; severe; dbSNP:rs119450940
- 447 S → P: in ADSLD; severe; dbSNP:rs777821034
- 450 T → S: in ADSLD; moderate; dbSNP:rs372895468
- 452 R → P: in ADSLD; severe; dbSNP:rs775671027
Q05911 Adenylosuccinate lyase; ASL; Adenylosuccinase; ASase; EC 4.3.2.2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
26% identity, 87% coverage: 4:379/434 of query aligns to 14:401/482 of Q05911
- K196 (≠ S184) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
G4VQX9 Adenylosuccinate lyase; ADSL; Adenylosuccinase; SmADSL; EC 4.3.2.2 from Schistosoma mansoni (Blood fluke) (see paper)
25% identity, 88% coverage: 1:382/434 of query aligns to 11:403/480 of G4VQX9
- R14 (= R4) binding AMP
- Y15 (= Y5) binding AMP
- R79 (≠ K69) binding AMP
- H80 (= H70) binding AMP
- D81 (= D71) binding AMP
- Q236 (= Q215) binding AMP
- R298 (≠ N279) binding AMP
- S329 (= S309) binding AMP
- R333 (= R313) binding AMP
5vkwB Crystal structure of adenylosuccinate lyase ade13 from candida albicans
25% identity, 87% coverage: 4:379/434 of query aligns to 14:394/469 of 5vkwB
Sites not aligning to the query:
5nxaA Crystal structure of neanderthal adenylosuccinate lyase (adsl)in complex with its products aicar and fumarate (see paper)
23% identity, 87% coverage: 4:379/434 of query aligns to 13:384/464 of 5nxaA
- active site: H79 (= H70), T151 (≠ S143), H152 (= H144), K275 (= K271), E282 (= E278)
- binding n-{[5-amino-1-(5-o-phosphono-beta-d-arabinofuranosyl)-1h-imidazol-4-yl]carbonyl}-l-aspartic acid: R13 (= R4), Y14 (= Y5), R78 (≠ K69), H79 (= H70), D80 (= D71), T104 (= T96), S105 (= S97), Q234 (= Q215), K275 (= K271), R283 (≠ N279), L311 (≠ I306), S314 (= S309), A315 (≠ S310), R318 (= R313)
5eytA Crystal structure of adenylosuccinate lyase from schistosoma mansoni in complex with amp (see paper)
23% identity, 88% coverage: 1:382/434 of query aligns to 9:395/472 of 5eytA