SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_010442783.1 NCBI__GCF_000192475.1:WP_010442783.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

3u9sF Crystal structure of p. Aeruginosa 3-methylcrotonyl-coa carboxylase (mcc) 750 kd holoenzyme, coa complex (see paper)
68% identity, 100% coverage: 3:535/535 of query aligns to 6:537/537 of 3u9sF

query
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3u9sF

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active site: A150 (= A147), A192 (= A189), G193 (= G190), F381 (= F379), A421 (= A419), G422 (= G420)
binding 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal: T379 (= T377), G380 (= G378), F381 (= F379), A421 (= A419), M447 (= M445)
binding coenzyme a: R49 (= R46), K115 (= K112), T118 (= T115), A150 (= A147), N151 (= N148), L152 (= L149)

8j4zJ Human 3-methylcrotonyl-coa carboxylase in bccp-cts state with substrate
66% identity, 95% coverage: 27:535/535 of query aligns to 33:541/541 of 8j4zJ

query
sites
8j4zJ

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binding 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal: L219 (= L212), L224 (= L217), G384 (= G378), F385 (= F379), Y423 (≠ F417), A425 (= A419)
binding ~{S}-[2-[3-[[(2~{R})-4-[[[(2~{S},3~{S},4~{S},5~{S})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] 3-methylbut-2-enethioate: R56 (= R50), K119 (= K112), G120 (= G113), A122 (≠ T115), G152 (= G145), G153 (= G146), A154 (= A147), Y155 (≠ N148), F169 (= F162), T195 (= T188), A196 (= A189), G197 (= G190), I463 (≠ V457), Q467 (≠ G461)

8j99B Human 3-methylcrotonyl-coa carboxylase in bcs-mcoa state
61% identity, 95% coverage: 27:535/535 of query aligns to 33:514/514 of 8j99B

query
sites
8j99B

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Y

Y

V

V

P

P

A

A

M

M

S

A

D

D

V

E

T

N

I

I

I

I

V

V

K

R

E

K

Q

-

G

-

T

-

I

-

F

-

L

-

A

-

G

-

P

-

P

-

L

-

V

-

K

-

A

-

A

-

T

-

G

-

E

-

V

-

V

-

S

-

A

-

E

-

D

-

L

-

G

-

G

-

G

A

D

D

V

L

H

H

T

C

R

R

L

K

S

S

G

G

V

V

A

S

D

D

Y

H

L

W

A

A

E

L

D

D

D

D

A

H

H

H

A

A

L

L

A

H

L

L

A

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R

R

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A

V

V

V

G

R

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N

L

L

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N

G

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-

K

K

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L

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A

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D

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L

L

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G

G

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I

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L

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V

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G

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K

K

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G

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L

L

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V

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G

G

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F

A

A

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C

Y

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P

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G

G

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I

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N

N

N

N

G

G

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V

L

L

F

F

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S

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A

S

A

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K

K

K

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G

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H

H

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V

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L

L

C

C

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C

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Q

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R

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N

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P

P

L

L

V

L

F

F

L

L

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Q

N

N

I

I

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G

G

F

F

M

M

V

V

G

G

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Y

Y

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A

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G

I

I

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G

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A

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K

M

M

V

V

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A

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V

A

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T

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K

K

I

I

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L

L

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V

I

G

G

G

G

S

S

F

Y

G

G

A

A

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G

N

N

Y

Y

G

G

M

M

A

C

G

G

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R

A

A

Y

Y

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S

P

P

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F

F

L

L

W

Y

T

I

W

W

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P

N

N

S

A

R

R

I

I

S

S

V

V

M

M

G

G

G

G

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Q

A

A

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V

L

L

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K

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D

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A

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A

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A

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A

A

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K

K

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P

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L

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K

M

K

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F

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P

P

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Y

Y

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R

L

V

W

W

D

D

D

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G

G

I

I

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R

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L

V

V

L

L

A

G

L

L

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S

L

F

S

S

A

A

A

A

L

L

N

N

A

A

P

P

I

I

E

E

E

K

T

T

R

D

F

F

G

G

V

I

F

F

R

R

M

M

binding ~{S}-[2-[3-[[(2~{R})-4-[[[(2~{S},3~{S},4~{S},5~{S})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] 3-methylbut-2-enethioate: R56 (= R50), K119 (= K112), G120 (= G113), G152 (= G145), A154 (= A147), Y155 (≠ N148), L156 (= L149)

8rthF Trypanosoma brucei 3-methylcrotonyl-coa carboxylase (see paper)
58% identity, 98% coverage: 3:524/535 of query aligns to 22:542/542 of 8rthF

query
sites
8rthF

L

L

K

H

S

Y

K

P

A

A

I

E

P

S

T

T

S

N

E

P

G

E

F

F

K

K

A

A

N

N

R

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A

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A

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H

M

L

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A

A

L

L

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A

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A

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D

G

G

G

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-

A

-

D

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K

R

R

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A

R

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A

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H

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R

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G

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K

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L

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A

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L

L

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D

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F

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L

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G

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M

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Y

Y

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G

G

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C

C

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M

M

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A

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D

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K

G

G

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Y

Y

Y

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M

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V

K

K

K

K

H

H

L

L

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Q

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I

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A

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N

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L

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P

C

C

I

I

Y

Y

L

L

V

V

D

D

S

S

G

G

G

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A

A

N

N

L

L

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N

M

Q

Q

D

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D

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V

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F

P

P

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D

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H

H

F

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G

G

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I

I

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N

N

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Q

A

A

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M

M

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K

K

G

G

I

I

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Q

I

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T

V

V

M

M

G

G

S

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C

C

T

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A

A

G

G

G

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A

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Y

Y

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V

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A

M

M

S

S

D

D

V

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T

S

I

I

I

I

V

V

K

K

E

G

Q

N

G

G

T

T

I

I

F

F

L

L

A

G

G

G

P

P

P

P

L

L

V

V

K

F

A

A

A

A

T

T

G

G

E

E

V

E

V

V

S

T

A

P

E

E

D

E

L

L

G

G

G

G

G

A

D

D

V

V

H

H

T

C

R

R

L

A

S

S

G

G

V

V

A

T

D

D

Y

Y

L

F

A

A

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D

D

D

D

A

L

H

H

A

A

L

L

A

Y

L

L

A

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R

R

R

R

A

I

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V

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A

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N

L

L

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N

G

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A

N

G

D

L

C

K

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P

L

C

R

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G

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A

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P

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L

Y

Y

D

D

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P

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E

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I

L

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G

G

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F

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M

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A

A

D

D

L

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K

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D

D

I

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R

R

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A

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V

I

I

A

A

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R

L

L

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V

D

D

G

G

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F

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D

D

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K

K

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G

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T

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L

L

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V

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C

G

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A

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G

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M

P

L

V

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G

G

I

I

I

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N

N

N

G

G

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I

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L

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A

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K

K

G

G

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H

H

F

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V

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L

L

C

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H

R

R

K

N

T

I

P

P

L

L

V

L

F

F

L

L

Q

Q

N

N

I

I

T
|
T

G
|
G

F
|
F

M

M

V

V

G

G

R

K

K

T

Y

Y

E

E

N

E

E

G

G

G

I

I

A

A

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K

H

N

G

G

A

A

K

K

M

L

V

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A

A

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H

V

V

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P

K

K

I

I

T

T

M

I

L

I

V

I

G

G

G

G

S

S

F

Y

G

G

A

A

G

G

N

N

Y

Y

G

G

M

M

A

C

G

G

R

R

A

A

Y

F

Q

G

P

P

R

R

F

F

L

L

W

F

T

M

W

W

P

P

N

N

S

A

R

R

I

I

S

S

V

V

M

M

G

G

G

G

E
x
N

Q
|
Q

A

A

A

A

G

T

V

V

L

L

A

A

T

L

V

T

K

N

R

S

D

K

G

L

I

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E

R

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Q

-

G

-

G

-

T

-

W

-

S

-

A

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N

E

E

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V

A

Q

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D

F

F

K

K

R

A

P

K

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V

L

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M

K

F

Y

E

E

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Q

E

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G

H

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P

C

L

Y

Y

Y

A

S

S

T

A

A

R

R

L

L

W

W

D

D

D

D

G

G

I

V

I

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P

P

R

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K

D

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R

R

D

A

V

V

L

V

A

V

L

Q

S

A

L

L

S

L

A

S

A

T

L

L

N

S

A

A

P

P

binding 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal: T403 (= T377), G404 (= G378), F405 (= F379), N474 (≠ E448), Q475 (= Q449)

8f3dA 3-methylcrotonyl-coa carboxylase in filament, beta-subunit centered (see paper)
58% identity, 90% coverage: 41:524/535 of query aligns to 76:566/566 of 8f3dA

query
sites
8f3dA

E

D

R

R

S

V

R

R

A

K

R

L

H

H

E

L

S

S

R

R

G

G

K

K

M

M

L

L

P

A

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R

R

D

R

R

V

I

A

E

N

R

L

L

L

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D

D

P

P

G

G

S

T

P

R

F

F

L

L

E

E

I

L

G

S

A

Q

T

L

A

A

A

G

H

W

G

D

M

L

Y

Y

G

W

G

D

A

D

A

K

P

K

-

K

-

E

-

Y

-

E

-

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C

-

Y

C

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A

G

G

G

V

I

I

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A

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G

G

I

I

G

G

R

L

V

V

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N

G

G

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R

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C

M

M

V

L

V

V

C

A

N

N

D

D

A

A

T

T

V

V

K

K

G

G

G

G

T

T

Y

Y

Y

Y

P

P

M

I

T

T

V

V

K

K

K

K

H

H

L

L

R

R

A

A

Q

Q

E

K

I

I

A

A

E

E

E

Q

N

N

H

H

L

L

P

P

C

C

I

I

Y

Y

L

L

V

V

D

D

S

S

G

G

G

G

A

A

N

N

L

L

P

S

N

R

Q

Q

D

D

E

D

V

V

F

F

P

P

D

D

R

E

D

Q

H

H

F

F

G

G

R

R

I

I

F

F

Y

Y

N

N

Q

E

A

A

R

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M

M

S

S

A

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K

K

G

S

I

I

A

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Q

I

I

A

A

V

V

V

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M

M

G

G

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C

C

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A

A

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G

G

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A

A

Y

Y

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M

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A

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I

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G

G

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F

L

L

A

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G

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L

A

A

A

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G

G

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A

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D

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L

L

G

G

G

G

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A

D

D

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H

H

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C

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S

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G

G

V

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A

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D

D

Y

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L

Y

A

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D

D

D

D

A

L

H

H

A

A

L

L

A

Y

L

L

A

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R

R

R

A

A

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V

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A

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N

L

L

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G

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A

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L

L

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K

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G

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M

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D

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G

G

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F

F

D

D

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F

F

K

K

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A

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L

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Y

G

G

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N

T

T

L

L

V

V

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C

G

G

F

F

A

A

H

R

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I

K

E

G

G

C

M

P

Q

V

V

G

G

I

I

I

I

A

A

N

N

N

Q

G

G

V

I

L

L

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Y

S

S

E

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A

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A

A

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K

K

G

G

A

A

H

H

F

F

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L

L

C

C

S

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Q

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R

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N

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P

P

L

L

V

L

F

F

L

L

Q

Q

N

N

I

I

T

T

G

G

F
|
F

M

M

V

V

G

G

R

K

K

K

Y

Y

E

E

N

E

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G

G

G

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I

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N

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G

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M

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M

A

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K

K

I

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T

M

V

L

L

V

I

G

G

G

G

S

S

F

Y

G

G

A

A

G

G

N

N

Y

Y

G

G

M

M

A

C

G

G

R

R

A

A

Y

F

Q

E

P

P

R

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F

F

L

L

W

F

T

M

W

W

P

P

N

N

S

A

R

R

I

I

S

S

V

V

M

M

G

G

G

G

E

T

Q
|
Q

A

A

A

A

G

T

V

V

L

L

A

T

T

L

V

T

K

N

R

R

D

N

G

-

I

-

E

-

R

-

Q

-

G

L

G

K

T

N

W

A

S

S

A

E

E

A

E

E

E

I

A

A

E

A

F

F

K

K

R

D

P

K

T

V

L

K

D

K

M

K

F

Y

E

E

E

K

Q

E

S

G

H

S

P

C

L

Y

Y

Y

A

S

S

T

A

A

R

R

L

L

W

W

D

D

D

D

G

G

I

V

I

I

D

A

P

P

R

E

K

D

S

T

R

R

D

V

V

V

L

V

A

A

L

E

S

A

L

L

S

R

A

A

A

T

L

R

N

L

A

A

P

P

binding 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal: F426 (= F379), Q496 (= Q449)

1vrgA Crystal structure of propionyl-coa carboxylase, beta subunit (tm0716) from thermotoga maritima at 2.30 a resolution
32% identity, 93% coverage: 23:517/535 of query aligns to 5:495/515 of 1vrgA

query
sites
1vrgA

D

D

A

K

L

I

A

E

R

E

I

L

S

K

E

K

A

I

A

E

E

K

A

E

A

I

R

E

M

Q

G

G

G

G

G

G

E

P

R

E

S

K

R

V

A

E

R

K

H

Q

E

H

S

R

R

A

G

G

K

K

M

L

L

T

P

A

R

W

R

E

R

R

V

L

A

E

N

L

L

L

L

L

D

D

P

P

G

G

S

T

P

-

F

F

L

V

E

E

I

I

G

D

A

K

T

F

A

V

A

E

H

H

-

R

-

N

-

T

-

Y

-

F

G

G

M

L

Y

D

G

K

G

V

A

K

A

L

P

P

C

R

A

D

G

G

V

V

I

I

A

T

G

G

I

V

G

G

R

E

V

I

Q

N

G

G

R

R

E

K

V

V

M

A

V

V

V

F

C

S

N

Q

D

D

A

F

T

T

V

V

K

M

G

G

G

G

T

S

Y

L

Y

G

P

E

M

M

T

H

V

A

K

K

K

K

H

I

L

V

R

K

A

L

Q

L

E

D

I

L

A

A

E

L

E

K

N

M

H

G

L

I

P

P

C

V

I

I

Y

G

L

I

V

N

D

D

S

S

G

G

G

G

A
|
A

N

R

L

I

P

Q

N

E

Q

G

D

V

E

D

V

A

F

L

P

A

D

G

R

-

D

-

H

-

F

Y

G

G

R

E

I

I

F

F

Y

L

N

R

Q

N

A

T

R

-

M

L

S

A

A

S

K

G

G

V

I

V

A

P

Q

Q

I

I

A

T

V

V

V

I

M

A

G

G

S

P

C

C

T

A

A
x
G

G
|
G

G

A

A

V

Y

Y

V

S

P

P

A

A

M

L

S

T

D

D

V

F

T

I

I

V

I

M

V

V

K

D

E

Q

Q

T

G

A

T

R

I

M

F

F

L

I

A

T

G
|
G

P

P

P
x
N

L
x
V

V

I

K

K

A

A

A

V

T

T

G

G

E

E

V

E

V

I

S

S

A

Q

E

E

D

D

L

L

G

G

G

G

G

A

D

M

V

V

H

H

T

N

R

Q

L

K

S

S

G

G

V

N

A

A

D

H

Y

F

L

L

A

A

E

D

D

N

D

D

A

E

H

K

A

A

L

M

A

S

L

L

A

V

R

R

R

T

A

L

V

L

G

S

S

Y

L

L

G

-

G

-

A

-

G

-

L

-

K

-

P

P

G

S

L

N

R

N

F

A

E

E

A

E

S

P

-

P

-

V

E

E

E

D

P

P

D

D

Y

T

D

S

-

L

-

E

-

T

P

P

E

E

E

D

V

I

L

L

G

D

V

I

V

L

P

P

A

D

D

N

L

P

R

N

T

K

P

G

Y

Y

D

D

I

V

R

R

E

D

V

V

I

I

A

K

R

R

L

V

V

V

D

D

G

H

S

G

R

E

F

F

D

F

E

E

F

V

K

Q

P

P

R

Y

F

F

G

A

E

K

T

N

L

I

V

V

T

I

G

G

F

F

A

A

H

R

V

I

K

Q

G

G

C

K

P

T

V

V

G

G

I

I

I

V

A

A

N

N

N

Q

-

P

-

S

-

V

-

L

-

A

G

G

V

V

L

L

F

D

S

I

E

D

A

S

A

S

Q

D

K

K

G

A

A

A

H

R

F

F

V

I

E

R

L

F

C

L

S

D

Q

A

R

F

K

N

T

I

P

P

L

I

V

L

F

T

L

F

Q

V

N

D

I

T

T

P

G

G

F
x
Y

M

L

V

P

G

G

R

V

K

A

Y

Q

E

E

N

H

E

G

G

G

I

I

A

I

R

R

H

H

G

G

A

A

K

K

M

L

V

L

T

Y

A

A

V

Y

A

S

T

E

T

A

S

T

V

V

P

P

K

K

I

I

T

T

M

V

L

I

V

L

G

R

G

K

S

A

F

Y

G

G

A
x
G

G
x
A

N

Y

Y

I

G

A

M

M

A

G

G

S

R

K

A

H

Y

L

Q

G

P

A

R

D

F

M

L

V

W

L

T

A

W

W

P

P

N

S

S

A

R

E

I

I

S

A

V

V

M

M

G

G

G

P

E

E

Q

G

A

A

A

A

G

N

V

I

L

I

A

-

T

-

V

-

K

-

R

-

D

-

G

-

I

F

E

K

R

R

Q

E

G

I

G

E

T

A

W

S

S

S

A

N

E

P

E

E

E

E

A

T

E

-

F

-

K

R

R

R

P

K

T

L

L

I

D

E

M

E

F

Y

E

K

E

Q

Q

Q

-

F

S

A

H

N

P

P

L

Y

Y

I

A

A

S

A

A

S

R

R

L

G

W

Y

D

V

D

D

G

M

I

V

I

I

D

D

P

P

R

R

K

E

S

T

R

R

D

K

V

Y

L

I

A

M

L

R

S

A

L

L

active site: A133 (= A147), G171 (≠ A189), G172 (= G190), Y365 (≠ F379), G405 (≠ A419), A406 (≠ G420)
binding bicarbonate ion: G196 (= G214), N198 (≠ P216), V199 (≠ L217)

Q168G2 Propionyl-CoA carboxylase beta chain; EC 6.4.1.3 from Roseobacter denitrificans (strain ATCC 33942 / OCh 114) (Erythrobacter sp. (strain OCh 114)) (Roseobacter denitrificans) (see paper)
33% identity, 94% coverage: 23:526/535 of query aligns to 3:499/510 of Q168G2

query
sites
Q168G2

D

D

A

I

L

L

A

E

R

Q

I

L

S

E

E

D

A

R

A

R

E

A

A

A

A

A

R

R

M

L

G

G

G

G

G

G

E

Q

R

K

S

R

R

I

A

D

R

A

H

Q

E

H

S

G

R

R

G

G

K

K

M

L

L

T

P

A

R

R

R

E

R

R

V

V

A

D

N

L

L

L

L

L

D

D

P

E

G

G

S

S

P

-

F

F

L

E

E

E

I

F

G

D

A

M

T

F

A

V

A

T

H

H

-

R

-

C

-

T

-

D

-

F

G

N

M

M

Y

Q

G

D

G

Q

A

K

A

P

P

A

C

G

A

D

G

G

V

V

I

V

A

T

G

G

I

W

G
|
G

R

T

V

I

Q

N

G

G

R

R

E

V

V

V

M

Y

V

V

V

F

C

S

N

Q

D

D

A

F

T

T

V

V

K

L

G

G

G

G

T

S

Y

V

Y

S

P

E

M

T

T

H

V

S

K

K

K

K

H

I

L

C

R

K

A

I

Q

M

E

D

I

M

A

A

E

M

E

Q

N

N

H

G

L
x
A

P

P

C

V

I

I

Y

G

L

I

V

N

D

D

S

S

G

G

G

G

A

A

N

R

L

I

P

-

N

-

Q

-

D

Q

E

E

V

G

F

V

P

D

D

S

R

L

D

A

H

G

F

Y

G

G

R

E

I

V

F

F

Y

-

N

-

Q

Q

A

R

R

N

M

I

S

M

A

A

K

S

G

G

I

V

A

V

-

P

Q

Q

I

I

A

S

V

M

V

I

M

M

G

G

S

P

C

C

T

A

A

G

G

G

G

A

A

V

Y

Y

V

S

P

P

A

A

M

M

S

T

D

D

V

F

T

I

I

F

I

M

V

V

K

K

E

D

Q

S

G

S

T

Y

I

M

F

F

L

V

A

T

G

G

P

P

P

D

L

V

V

V

K

K

A

T

A

V

T

T

G

N

E

E

V

Q

V

V

S

S

A

A

E

E

D

E

L

L

G

G

G

G

G

A

D

T

V

T

H

H

T

T

R

R

L

K

S

S

G

S

V

V

A

A

D

D

Y

A

L

A

A

F

E

E

D

N

D

D

A

V

H

E

A

A

L

L

A

A

L

E

A

V

R

R

R

R

A

L

V

V

G

D

S

F

L

L

G

P

-

L

G

N

A

N

G

R

L

E

K

K

P

P

G

P

L

V

R

R

F

-

E

P

A

F

S

F

E

D

E

D

P

P

D

D

Y

R

D

I

P

E

E

P

E

S

V

L

L

D

G

T

V

L

V

V

P

P

A

D

D

N

L

P

R

N

T

T

P

P

Y

Y

D

D

I

M

R

K

E

E

V

L

I

I

A

H

R

K

L

L

V

A

D

D

G

E

S

G

R

D

F

F

D

Y

E

E

F

I

K

Q

P

E

R

E

F

F

G

A

E

K

T

N

L

I

V

I

T

T

G

G

F

F

A

I

H

R

V

L

K

E

G

G

C

R

P

T

V

V

G

G

I

V

I

V

A

A

N

N

N

Q

-

P

-

L

-

V

-

L

-

A

G

G

V

C

L

L

F

D

S

I

E

D

A

S

A

S

Q

R

K

K

G

A

A

A

H

R

F

F

V

V

E

R

L

F

C

C

S

D

Q

A

R

F

K

E

T

I

P

P

L

L

V

L

F

T

L

L

Q

I

N

D

I

V

T

P

G

G

F

F

M

L

V

P

G

G

R

T

K

S

Y

Q

E

E

N

Y

E

G

G

G

I

V

A

I

R

K

H

H

G

G

A

A

K

K

M

L

V

L

T

Y

A

A

V

Y

A

G

T

E

T

A

S

T

V

V

P

P

K

M

I

V

T

T

M

V

L

I

V

T

G

R

G

K

S

A

F

Y

G

G

A

G

G

A

N

Y

Y

V

G

V

M

M

A

S

G

S

R

K

A

H

Y

L

Q

R

P

A

R

D

F

F

L

N

W

Y

T

A

W

W

P

P

N

T

S

A

R

E

I

V

S

A

V

V

M

M

G

G

G

A

E

K

Q

G

A

A

A

T

G

E

V

I

L

I

A

-

T

-

V

-

K

-

R

-

D

-

G

-

I

-

E

-

R

H

Q

R

G

G

G

D

T

L

W

G

S

D

A

P

E

E

E

K

E

I

A

A

E

Q

F

H

K

-

R

-

P

-

T

T

L

A

D

D

M

Y

F

E

E

E

E

R

Q

F

S

A

H

N

P

P

L

F

Y

V

A

A

S

S

A

E

R

R

L

G

W

F

D

V

D

D

G

E

I

V

I

I

D

Q

P

P

R

R

K

S

S

T

R

R

D

K

V

R

L

V

A

A

L

R

S

A

L

F

S

A

A

S

A

L

L

R

N

N

A

K

P

S

I

V

E

Q

G79 (= G95) mutation to D: Loss of solubility.
A120 (≠ L136) mutation to P: Loss of solubility.

1on3C Transcarboxylase 12s crystal structure: hexamer assembly and substrate binding to a multienzyme core (with methylmalonyl-coenzyme a and methylmalonic acid bound) (see paper)
34% identity, 92% coverage: 25:517/535 of query aligns to 9:490/510 of 1on3C

query
sites
1on3C

L

V

A

E

R

Q

I

L

S

A

E

E

A

Q

A

R

E

Q

A

V

A

I

R

E

M

A

G

G

G

G

G

G

E

E

R

R

S
x
R

R

V

A

E

R

K

H

Q

E

H

S

S

R

Q

G

G

K

K

M

Q

L

T

P

A

R

R

R

E

R

R

V

L

A

N

N

N

L

L

L

L

D

D

P

P

G

H

S

S

P

-

F

F

L

D

E

E

I

V

G

G

A

A

T

F

A

R

A

K

H

H

-

R

-

T

-

T

-

L

-

F

G

G

M

M

Y

D

G

K

G

A

A

V

A

V

P

P

C

A

A

D

G

G

V

V

I

V

A

T

G

G

I

R

G

G

R

T

V

I

Q

L

G

G

R

R

E

P

V

V

M

H

V

A

V

A

C

S

N

Q

D

D

A
x
F

T

T

V

V

K

M

G
|
G

G

G

T
x
S

Y

A

Y

G

P

E

M

T

T

Q

V

S

K

T

K

K

H

V

L

V

R

E

A

T

Q

M

E

E

I

Q

A

A

E

L

E

L

N

T

H

G

L

T

P

P

C

F

I

L

Y

F

L

F

V

Y

D

D

S

S

G
|
G

G

G

A
|
A

N

R

L
x
I

P

-

N

-

Q

-

D

Q

E

E

V

G

F

I

P

D

D

S

R

L

D

S

H

G

F

Y

G

G

R

K

I

M

F

F

Y

F

N

A

Q

N

A

V

R

K

M

L

S

S

A

G

K

V

G

-

I

V

A

P

Q

Q

I

I

A

A

V

I

V

I

M

A

G

G

S

P

C

C

T

A

A
x
G

G
|
G

G

A

A

S

Y

Y

V

S

P

P

A

A

M

L

S

T

D

D

V

F

T

I

I

I

I

M

V

T

K

K

E

-

Q

K

G

A

T

H

I

M

F

F

L

I

A

T

G
|
G

P

P

P

Q

L
x
V

V

I

K

K

A

S

A

V

T

T

G

G

E

E

V

D

V

V

S

T

A

A

E

D

D

E

L

L

G

G

G

G

G

A

D

E

V

A

H

H

T

M

R

A

L

I

S

S

G

G

V

N

A

I

D

H

Y

F

L

V

A

A

E

E

D

D

D

D

A

D

H

A

A

A

L

E

A

L

L

I

A

A

R

K

R

K

A

L

V

L

G

S

S

F

L

L

G

P

G

Q

A

N

G

N

L

T

K

E

P

E

G

A

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F

F

V

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N

A

P

S

N

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N

E

-

P

-

D

D

Y

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D

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P

P

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N

-

T

E

E

V

L

L

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I

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D

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K

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Y

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D

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V

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I

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A

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V

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D

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W

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Y

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L

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E

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K

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A

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Y

G

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T

T

N

L

L

V

V

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T

G

A

F

F

A

A

H

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V

V

K

N

G

G

C

R

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S

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V

G

G

I

I

I

V

A

A

N

N

-

Q

-

P

-

S

-

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-

M

N

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G

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C

L

L

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A

A

A

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D

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K

G

A

A

A

H

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F

F

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V

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N

L

F

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C

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L

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D

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P

G

G

F
|
F

M

L

V

P

G

G

R

V

K

Q

Y

Q

E

E

N

Y

E

G

G

G

I

I

A

I

R

R

H

H

G

G

A

A

K

K

M

M

V

L

T

Y

A

A

V

Y

A

S

T

E

T

A

S

T

V

V

P

P

K

K

I

I

T

T

M

V

L

V

V

L

G

R

G

K

S

A

F

Y

G
|
G

A
x
G

G
x
S

N

Y

Y

L

G

A

M

M

A

C

G

N

R

R

A

D

Y

L

Q

G

P

A

R

D

F

A

L

V

W

Y

T

A

W

W

P

P

N

S

S

A

R

E

I

I

S

A

V

V

M
|
M

G

G

G

A

E

E

Q

G

A

A

A

A

G

N

V

V

L

I

A

F

T
x
R

V

K

K

E

R

P

D

D

G

A

I

M

E

R

R

-

Q

-

G

-

G

-

T

-

W

-

S

-

A

A

E

E

E

K

E

I

A

E

E

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F

Y

K

Q

R

N

P

A

T

F

L

-

D

-

M

-

F

-

E

-

E

-

Q

-

S

N

H

T

P

P

L

Y

Y

V

A

A

S

A

A

A

R

R

L

G

W

Q

D

V

D

D

G

D

I

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I

I

D

D

P

P

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A

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A

L

L

active site: A135 (= A147), G173 (≠ A189), G174 (= G190), F366 (= F379), G406 (≠ A419), S407 (≠ G420)
binding methylmalonic acid: G197 (= G214), V200 (≠ L217)
binding methylmalonyl-coenzyme a: R27 (≠ S43), F97 (≠ A109), G101 (= G113), S103 (≠ T115), G133 (= G145), A135 (= A147), I137 (≠ L149), G173 (≠ A189), G174 (= G190), G405 (= G418), G406 (≠ A419), M432 (= M445), R443 (≠ T456)

3n6rB Crystal structure of the holoenzyme of propionyl-coa carboxylase (pcc) (see paper)
33% identity, 94% coverage: 25:526/535 of query aligns to 1:495/506 of 3n6rB

query
sites
3n6rB

L

L

A

E

R

Q

I

L

S

E

E

D

A

R

A

R

E

A

A

A

A

A

R

R

M

L

G

G

G

G

G

G

E

Q

R

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S

R

R

I

A

D

R

A

H

Q

E

H

S

G

R

R

G

G

K

K

M

L

L

T

P

A

R

R

R

E

R

R

V

V

A

D

N

L

L

L

L

L

D

D

P

E

G

G

S

S

P

-

F

F

L

E

E

E

I

F

G

D

A

M

T

F

A

V

A

T

H

H

-

R

-

C

-

T

-

D

-

F

G

N

M

M

Y

Q

G

D

G

Q

A

K

A

P

P

A

C

G

A

D

G

G

V

V

I

V

A

T

G

G

I

W

G

G

R

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I

Q

N

G

G

R

R

E

V

V

V

M

Y

V

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V

F

C

S

N

Q

D

D

A

F

T

T

V

V

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L

G

G

G

G

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Y

V

Y

S

P

E

M

T

T

H

V

S

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K

K

K

H

I

L

C

R

K

A

I

Q

M

E

D

I

M

A

A

E

M

E

Q

N

N

H

G

L

A

P

P

C

V

I

I

Y

G

L

I

V

N

D

D

S

S

G

G

G

G

A
|
A

N

R

L

I

P

-

N

-

Q

-

D

Q

E

E

V

G

F

V

P

D

D

S

R

L

D

A

H

G

F

Y

G

G

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E

I

V

F

F

Y

-

N

-

Q

Q

A

R

R

N

M

I

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M

A

A

K

S

G

G

I

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A

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P

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x
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F

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G

P

P

P

D

L

V

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V

K

K

A

T

A

V

T

T

G

N

E

E

V

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V

V

S

S

A

A

E

E

D

E

L

L

G

G

G

G

G

A

D

T

V

T

H

H

T

T

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R

L

K

S

S

G

S

V

V

A

A

D

D

Y

A

L

A

A

F

E

E

D

N

D

D

A

V

H

E

A

A

L

L

A

A

L

E

A

V

R

R

R

R

A

L

V

V

G

D

S

F

L

L

G

P

-

L

G

N

A

N

G

R

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E

K

K

P

P

G

P

L

V

R

R

F

-

E

P

A

F

S

F

E

D

E

D

P

P

D

D

Y

R

D

I

P

E

E

P

E

S

V

L

L

D

G

T

V

L

V

V

P

P

A

D

D

N

L

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N

T

T

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P

Y

Y

D

D

I

M

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K

E

E

V

L

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I

A

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D

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Y

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R

E

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F

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A

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K

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L

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G

G

F

F

A

I

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L

K

E

G

G

C

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T

V

V

G

G

I

V

I

V

A

A

N

N

N

Q

-

P

-

L

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-

L

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A

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C

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L

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G

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F
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M

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x
P

G

G

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T

K

S

Y

Q

E

E

N

Y

E

G

G

G

I

V

A

I

R

K

H

H

G

G

A

A

K

K

M

L

V

L

T

Y

A

A

V

Y

A

G

T

E

T

A

S

T

V

V

P

P

K

M

I

V

T

T

M

V

L

I

V

T

G

R

G

K

S

A

F

Y

G

G

A
x
G

G
x
A

N

Y

Y

V

G

V

M

M

A

S

G

S

R

K

A

H

Y

L

Q

R

P

A

R

D

F

F

L

N

W

Y

T

A

W

W

P

P

N

T

S

A

R

E

I

V

S

A

V

V

M

M

G

G

G

A

E

K

Q

G

A

A

A

T

G

E

V

I

L

I

A

-

T

-

V

-

K

-

R

-

D

-

G

-

I

-

E

-

R

H

Q

R

G

G

G

D

T

L

W

G

S

D

A

P

E

E

E

K

E

I

A

A

E

Q

F

H

K

-

R

-

P

-

T

T

L

A

D

D

M

Y

F

E

E

E

E

R

Q

F

S

A

H

N

P

P

L

F

Y

V

A

A

S

S

A

E

R

R

L

G

W

F

D

V

D

D

G

E

I

V

I

I

D

Q

P

P

R

R

K

S

S

T

R

R

D

K

V

R

L

V

A

A

L

R

S

A

L

F

S

A

A

S

A

L

L

R

N

N

A

K

P

S

I

V

E

Q

active site: A127 (= A147), G165 (≠ A189), G166 (= G190), F360 (= F379), G400 (≠ A419), A401 (≠ G420)
binding 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal: F360 (= F379), P362 (≠ V381)

1on3E Transcarboxylase 12s crystal structure: hexamer assembly and substrate binding to a multienzyme core (with methylmalonyl-coenzyme a and methylmalonic acid bound) (see paper)
34% identity, 92% coverage: 27:517/535 of query aligns to 15:500/520 of 1on3E

query
sites
1on3E

R

Q

I

L

S

A

E

E

A

Q

A

R

E

Q

A