SitesBLAST

165:174 (vs. 128:137, 80% identical) binding FAD
S174 (= S137) binding substrate
WIT 198:200 (= WIT 161:163) binding FAD
SR 222:223 (≠ SK 183:184) binding substrate
G250 (= G211) to A: in IVA; uncertain significance
Y277 (≠ A238) binding substrate
DLER 284:287 (≠ DYER 245:248) binding substrate
E286 (= E247) active site, Proton acceptor; mutation to D: Residual isovaleryl-CoA dehydrogenase activity.; mutation to G: Loss of isovaleryl-CoA dehydrogenase activity. Does not affect isovaleryl-CoA dehydrogenase activity; when associated with 407-E.; mutation to Q: Loss of isovaleryl-CoA dehydrogenase activity.
A291 (= A252) to V: in IVA; uncertain significance; dbSNP:rs886042098
R312 (= R273) binding FAD
Q323 (= Q284) binding FAD
I379 (≠ V340) to T: in IVA; uncertain significance
QCFGG 380:384 (≠ QALGG 341:345) binding FAD
R398 (= R359) to Q: in IVA; uncertain significance; dbSNP:rs1477527791
Y403 (≠ M364) to N: in IVA; uncertain significance
A407 (= A368) mutation to E: Does not affect isovaleryl-CoA dehydrogenase activity; when associated with 286-D.
AG 407:408 (= AG 368:369) binding substrate
TSE 409:411 (= TSE 370:372) binding FAD

Sites not aligning to the query:

1:32 modified: transit peptide, Mitochondrion

5lnxD Crystal structure of mmgc, an acyl-coa dehydrogenase from bacillus subtilis.
41% identity, 95% coverage: 17:383/387 of query aligns to 9:373/374 of 5lnxD

query
sites
5lnxD

L

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active site: L122 (≠ M130), T123 (≠ S131), G239 (≠ E247), E358 (≠ A368), K370 (≠ R380)
binding flavin-adenine dinucleotide: L122 (≠ M130), T123 (≠ S131), G128 (= G136), S129 (= S137), F153 (≠ W161), T155 (= T163), R265 (= R273), Q267 (= Q275), F268 (= F276), I272 (= I280), N275 (≠ F283), I278 (≠ M286), Q331 (= Q341), I332 (≠ A342), G335 (= G345), Y357 (≠ G367), T360 (= T370), E362 (= E372)

5ol2F The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
42% identity, 95% coverage: 17:384/387 of query aligns to 11:378/378 of 5ol2F

query
sites
5ol2F

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active site: L124 (≠ M130), T125 (≠ S131), G241 (≠ E247), G374 (≠ R380)
binding calcium ion: E29 (= E35), E33 (≠ S39), R35 (≠ A41)
binding coenzyme a persulfide: L238 (= L244), R242 (= R248), E362 (≠ A368), G363 (= G369)
binding flavin-adenine dinucleotide: F122 (≠ L128), L124 (≠ M130), T125 (≠ S131), P127 (≠ S133), T131 (≠ S137), F155 (≠ W161), I156 (= I162), T157 (= T163), E198 (≠ F204), R267 (= R273), F270 (= F276), L274 (≠ I280), F277 (= F283), Q335 (= Q341), L336 (≠ A342), G338 (= G344), G339 (= G345), Y361 (≠ G367), T364 (= T370), E366 (= E372)

4l1fA Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
40% identity, 98% coverage: 6:383/387 of query aligns to 1:378/380 of 4l1fA

query
sites
4l1fA

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active site: L125 (≠ M130), T126 (≠ S131), G242 (≠ E247), E363 (≠ A368), R375 (= R380)
binding coenzyme a persulfide: T132 (≠ S137), H179 (≠ K184), F232 (≠ V237), M236 (= M241), E237 (≠ S242), L239 (= L244), D240 (= D245), R243 (= R248), Y362 (≠ G367), E363 (≠ A368), G364 (= G369), R375 (= R380)
binding flavin-adenine dinucleotide: F123 (≠ L128), L125 (≠ M130), T126 (≠ S131), G131 (= G136), T132 (≠ S137), F156 (≠ W161), I157 (= I162), T158 (= T163), R268 (= R273), Q270 (= Q275), F271 (= F276), I275 (= I280), F278 (= F283), L281 (≠ M286), Q336 (= Q341), I337 (≠ A342), G340 (= G345), I358 (≠ L363), Y362 (≠ G367), T365 (= T370), Q367 (≠ E372)
binding 1,3-propandiol: L5 (= L10), Q10 (≠ N15)

1jqiA Crystal structure of rat short chain acyl-coa dehydrogenase complexed with acetoacetyl-coa (see paper)
40% identity, 97% coverage: 9:383/387 of query aligns to 6:380/384 of 1jqiA

query
sites
1jqiA

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active site: G377 (≠ R380)
binding acetoacetyl-coenzyme a: L95 (= L98), F125 (≠ L128), S134 (= S137), F234 (≠ V237), M238 (= M241), Q239 (≠ S242), L241 (= L244), D242 (= D245), R245 (= R248), Y364 (≠ G367), E365 (≠ A368), G366 (= G369)
binding flavin-adenine dinucleotide: F125 (≠ L128), L127 (≠ M130), S128 (= S131), G133 (= G136), S134 (= S137), W158 (= W161), T160 (= T163), R270 (= R273), F273 (= F276), L280 (≠ F283), Q338 (= Q341), I339 (≠ A342), G342 (= G345), I360 (≠ L363), T367 (= T370), E369 (= E372), I370 (= I373)

P15651 Short-chain specific acyl-CoA dehydrogenase, mitochondrial; SCAD; Butyryl-CoA dehydrogenase; EC 1.3.8.1 from Rattus norvegicus (Rat) (see 2 papers)
40% identity, 97% coverage: 9:383/387 of query aligns to 33:407/412 of P15651

query
sites
P15651

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152:161 (vs. 128:137, 50% identical) binding FAD
S161 (= S137) binding substrate
WIT 185:187 (= WIT 161:163) binding FAD
DMGR 269:272 (≠ DYER 245:248) binding substrate
R297 (= R273) binding FAD
QILGG 365:369 (≠ QALGG 341:345) binding FAD
E392 (≠ A368) active site, Proton acceptor
TSE 394:396 (= TSE 370:372) binding FAD

Sites not aligning to the query:

1:24 modified: transit peptide, Mitochondrion

1ukwB Crystal structure of medium-chain acyl-coa dehydrogenase from thermus thermophilus hb8
39% identity, 98% coverage: 6:383/387 of query aligns to 1:377/379 of 1ukwB

query
sites
1ukwB

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active site: L124 (≠ M130), S125 (= S131), T241 (≠ E247), E362 (≠ A368), R374 (= R380)
binding cobalt (ii) ion: D145 (= D151), H146 (= H152)
binding flavin-adenine dinucleotide: F122 (≠ L128), L124 (≠ M130), S125 (= S131), G130 (= G136), S131 (= S137), W155 (= W161), S157 (≠ T163), K200 (= K206), L357 (= L363), Y361 (≠ G367), E362 (≠ A368), T364 (= T370), E366 (= E372), L370 (≠ M376)

1ukwA Crystal structure of medium-chain acyl-coa dehydrogenase from thermus thermophilus hb8
39% identity, 98% coverage: 6:383/387 of query aligns to 1:377/379 of 1ukwA

query
sites
1ukwA

M

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active site: L124 (≠ M130), S125 (= S131), T241 (≠ E247), E362 (≠ A368), R374 (= R380)
binding flavin-adenine dinucleotide: F122 (≠ L128), L124 (≠ M130), S125 (= S131), G130 (= G136), S131 (= S137), W155 (= W161), S157 (≠ T163), L357 (= L363), Y361 (≠ G367), E362 (≠ A368), T364 (= T370), E366 (= E372), L370 (≠ M376)

P16219 Short-chain specific acyl-CoA dehydrogenase, mitochondrial; SCAD; Butyryl-CoA dehydrogenase; EC 1.3.8.1 from Homo sapiens (Human) (see 3 papers)
40% identity, 97% coverage: 9:383/387 of query aligns to 33:407/412 of P16219

query
sites
P16219

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G90 (= G66) to S: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs121908005
E104 (= E80) natural variant: Missing (in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs387906308)
152:161 (vs. 128:137, 50% identical) binding in other chain
R171 (≠ E147) to W: 69% of wild-type acyl-CoA dehydrogenase activity; confers susceptibility to ethylmalonicaciduria; dbSNP:rs1800556
WIT 185:187 (= WIT 161:163) binding in other chain
A192 (= A168) to V: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs28940874
G209 (= G185) to S: 86% of wild-type acyl-CoA dehydrogenase activity; confers susceptibility to ethylmalonicaciduria; dbSNP:rs1799958
R297 (= R273) binding FAD
Q308 (= Q284) binding in other chain
R325 (= R301) to W: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs121908006
S353 (= S329) to L: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs28941773
QILGG 365:369 (≠ QALGG 341:345) binding FAD
R380 (= R356) to W: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs28940875
TSE 394:396 (= TSE 370:372) binding in other chain

Sites not aligning to the query:

1:24 modified: transit peptide, Mitochondrion

8sgsA Human liver mitochondrial short-chain specific acyl-coa dehydrogenase (see paper)
40% identity, 97% coverage: 9:383/387 of query aligns to 3:377/381 of 8sgsA

query
sites
8sgsA

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binding coenzyme a: S131 (= S137), A133 (≠ V139), N177 (≠ S183), F231 (≠ V237), M235 (= M241), L238 (= L244), I312 (≠ R318), E362 (≠ A368), G363 (= G369)
binding flavin-adenine dinucleotide: F122 (≠ L128), L124 (≠ M130), S125 (= S131), G130 (= G136), S131 (= S137), W155 (= W161), T157 (= T163), R267 (= R273), F270 (= F276), L274 (≠ I280), L277 (≠ F283), Q335 (= Q341), I336 (≠ A342), G338 (= G344), G339 (= G345), I357 (≠ L363), I360 (= I366), Y361 (≠ G367), T364 (= T370), E366 (= E372)

7y0bA Crystal structure of human short-chain acyl-coa dehydrogenase
40% identity, 97% coverage: 9:383/387 of query aligns to 6:380/385 of 7y0bA

query
sites
7y0bA

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Q

Q

K

K

Q

Q

K

A

Y

W

L

V

P

T

R

P

L

F

I

T

S

S

G

G

D

D

H

K

V

I

G

G

A

C

L
x
F

A

A

M
x
L

S
|
S

E

E

S

P

S

G

A

N

G
|
G

S
|
S

D

D

V

A

V

G

S

A

M

A

K

S

L

T

R

T

A

A

E

R

K

A

R

E

N

G

D

D

H

S

Y

W

R

V

L

L

N

N

G

G

N

T

K

K

Y

A

W
|
W

I

I

T
|
T

N

N

G

A

P

W

D

E

A

A

D

S

T

A

L

A

V

V

Y

F

A

A

K

S

T

T

D

D

P

R

E

A

A

L

G

Q

S

N

K

K

G

G

I

I

T

S

A

A

F

F

L

L

I

V

E

P

K

M

E

P

M

T

K

P

G

G

F

L

S

T

T

L

S

G

P

K

H

K

F

E

D

D

K

K

L

L

G

G

M

I

R

R

G

G

S

S

N

S

T

T

A

A

E

N

L

L

I

I

F

F

E

E

D

D

V

C

E

R

V

I

P

P

F

K

E

D

N

S

V

I

L

L

G

G

E

E

E

P

G

G

R

M

G

G

V

F

A

K

V

I

L

A

M

M

S

Q

G

T

L

L

D

D

Y

M

E

G

R

R

V

I

V

G

L

I

A

A

G

S

I

Q

G

A

T

L

G

G

I

I

M

A

A

Q

A

T

C

A

L

L

D

D

E

C

V

A

M

V

P

N

Y

Y

L

A

A

E

E

N

R
|
R

K

M

Q

A

F
|
F

G

G

K

A

P

P

I

L

G

T

S

K

F
x
L

Q

Q

L
x
V

M

I

Q

Q

G

F

K

K

I

L

A

A

D

D

M

M

Y

A

T

L

A

A

M

L

N

E

S

S

A

A

R

R

A

L

Y

L

V

T

Y

W

E

R

V

A

A

A

K

M

A

L

C

K

D

D

R

N

G

K

D

K

V

P

T

F

R

I

Q

K

D

E

A

A

A

M

C

A

C

K

L

L

Y

A

A

A

S

S

E

E

Q

A

A

A

M

T

V

A

Q

I

A

S

H

H

Q

Q

A

A

V

I

Q
|
Q

A
x
I

L

L

G

G

G
|
G

A
x
M

G

G

F

Y

L

V

S
x
T

D
x
E

S

M

P

P

V

A

S

E

R

R

I

H

F

Y

R

R

D

D

A

A

K

R

L
x
I

M

T

E

E

I

I

G
x
Y

A

E

G

G

T
|
T

S

S

E
|
E

I
|
I

R

Q

R

R

M
x
L

L

V

I

I

G

A

R

G

E

H

M

L

binding (2S,3R,4R,5S,6R)-2-[4-chloranyl-3-[[4-[(3S)-oxolan-3-yl]oxyphenyl]methyl]phenyl]-6-(hydroxymethyl)oxane-3,4,5-triol: M343 (≠ A346), T347 (≠ S350), E348 (≠ D351)
binding flavin-adenine dinucleotide: F125 (≠ L128), L127 (≠ M130), S128 (= S131), G133 (= G136), S134 (= S137), W158 (= W161), T160 (= T163), R270 (= R273), F273 (= F276), L280 (≠ F283), V282 (≠ L285), Q338 (= Q341), I339 (≠ A342), G342 (= G345), I360 (≠ L363), Y364 (≠ G367), T367 (= T370), E369 (= E372), I370 (= I373), L373 (≠ M376)

7y0aC Crystal structure of human short-chain acyl-coa dehydrogenase
40% identity, 97% coverage: 9:383/387 of query aligns to 9:383/387 of 7y0aC

query
sites
7y0aC

D

E

L

L

G

P

E

E

D

T

V

H

N

Q

A

M

L

L

R

L

E

Q

M

T

V

C

H

R

R

D

W

F

A

A

Q

E

E

K

R

E

V

L

K

F

P

P

M

I

A

A

E

Q

I

V

D

D

A

K

S

E

N

H

A

L

F

F

P

P

N

A

E

A

L

Q

W

V

K

K

E

K

M

M

G

G

D

G

L

L

G

G

L

L

G

A

V

M

T

D

V

V

P

P

E

E

Y

L

G

G

A

A

G

G

M

L

S

D

Y

Y

L

L

A

A

H

Y

T

A

I

I

A

A

V

M

E

E

V

I

A

S

R

R

A

G

S

C

A

A

S

S

V

T

S

G

L

V

S

I

Y

M

G

S

A

V

H

N

S

N

N

S

L

L

C

Y

V

L

N

G

Q

P

I

I

K

L

L

K

N

F

G

G

T

S

D

K

E

E

Q

Q

K

K

Q

Q

K

A

Y

W

L

V

P

T

R

P

L

F

I

T

S

S

G

G

D

D

H

K

V

I

G

G

A

C

L
x
F

A

A

M
x
L

S
|
S

E

E

S

P

S

G

A

N

G
|
G

S
|
S

D

D

V

A

V

G

S

A

M

A

K

S

L

T

R

T

A

A

E

R

K

A

R

E

N

G

D

D

H

S

Y

W

R

V

L

L

N

N

G

G

N

T

K

K

Y

A

W
|
W

I

I

T
|
T

N

N

G

A

P

W

D

E

A

A

D

S

T

A

L

A

V

V

Y

F

A

A

K

S

T

T

D

D

P

R

E

A

A

L

G

Q

S

N

K

K

G

G

I

I

T

S

A

A

F

F

L

L

I

V

E

P

K

M

E

P

M

T

K

P

G

G

F

L

S

T

T

L

S

G

P

K

H

K

F

E

D

D

K

K

L

L

G

G

M

I

R

R

G

G

S

S

N

S

T
|
T

A

A

E

N

L

L

I

I

F

F

E

E

D

D

V

C

E

R

V

I

P

P

F

K

E

D

N

S

V

I

L

L

G

G

E

E

E

P

G

G

R

M

G

G

V

F

A

K

V

I

L

A

M

M

S

Q

G

T

L

L

D

D

Y

M

E

G

R

R

V

I

V

G

L

I

A

A

G

S

I

Q

G

A

T

L

G

G

I

I

M

A

A

Q

A

T

C

A

L

L

D

D

E

C

V

A

M

V

P

N

Y

Y

L

A

A

E

E

N

R
|
R

K

M

Q

A

F
|
F

G

G

K

A

P

P

I
x
L

G

T

S

K

F
x
L

Q

Q

L
x
V

M

I

Q

Q

G

F

K

K

I

L

A

A

D

D

M

M

Y

A

T

L

A

A

M

L

N

E

S

S

A

A

R

R

A

L

Y

L

V

T

Y

W

E

R

V

A

A

A

K

M

A

L

C

K

D

D

R

N

G

K

D

K

V

P

T

F

R

I

Q

K

D

E

A

A

A

M

C

A

C

K

L

L

Y

A

A

A

S

S

E

E

Q

A

A

A

M

T

V

A

Q

I

A

S

H

H

Q

Q

A

A

V

I

Q
|
Q

A
x
I

L

L

G

G

G
|
G

A

M

G

G

F

Y

L

V

S

T

D

E

S

M

P

P

V

A

S

E

R

R

I

H

F

Y

R

R

D

D

A

A

K

R

L
x
I

M

T

E

E

I

I

G
x
Y

A

E

G

G

T
|
T

S

S

E
|
E

I

I

R

Q

R

R

M
x
L

L

V

I

I

G

A

R

G

E

H

M

L

binding flavin-adenine dinucleotide: F128 (≠ L128), L130 (≠ M130), S131 (= S131), G136 (= G136), S137 (= S137), W161 (= W161), T163 (= T163), T214 (= T214), R273 (= R273), F276 (= F276), L280 (≠ I280), L283 (≠ F283), V285 (≠ L285), Q341 (= Q341), I342 (≠ A342), G345 (= G345), I363 (≠ L363), Y367 (≠ G367), T370 (= T370), E372 (= E372), L376 (≠ M376)

3pfdC Crystal structure of an acyl-coa dehydrogenase from mycobacterium thermoresistibile bound to reduced flavin adenine dinucleotide solved by combined iodide ion sad mr (see paper)
40% identity, 95% coverage: 16:383/387 of query aligns to 3:368/369 of 3pfdC

query
sites
3pfdC

A

A

L

L

R

R

E

E

M

A

V

I

H

R

R

A

W

L

A

A

Q

E

E

K

R

E

V

I

K

A

P

P

M

Y

A

A

E

E

I

V

D

D

A

E

S

K

N

A

A

R

F

F

P

P

N

E

E

E

L

A

W

L

K

A

E

A

M

L

G

N

D

S

L

S

G

G

L

F

L

S

G

A

V

I

T

H

V

V

P

P

E

E

Y

Y

G

G

A

Q

G

G

M

A

S

D

Y

S

L

V

A

A

H

T

T

C

I

I

A

V

V

I

E

E

V

V

A

A

R

R

A

V

S

D

A

C

S

S

V

A

S

S

L

L

S

-

Y

I

G

P

A

A

H

V

S

N

N

K

L

L

C

G

V

T

N

M

Q

G

I

L

K

I

L

L

N

R

G

G

T

S

D

E

E

E

Q

L

K

K

Q

K

K

Q

Y

V

L

L

P

P

R

A

L

V

I

A

S

S

G

G

D

E

H

A

V

M

G

A

A

S

L
x
Y

A

A

M
x
L

S
|
S

E

E

S

R

S

E

A

A

G
|
G

S
|
S

D

D

V

A

S

S

M

M

K

R

L

T

R

R

A

A

E

V

K

A

R

D

N

G

D

D

H

D

Y

W

R

I

L

L

N

N

G

G

N

S

K

K

Y

C

W
|
W

I
|
I

T
|
T

N

N

G

G

P

G

D

K

A

S

D

T

T

W

L

Y

V

T

V

V

Y

M

A

A

K

V

T

T

D

D

P

P

E

D

A

K

G

G

S

A

K

N

G

G

I

I

T

S

A

A

F

F

L

M

I

V

E

H

K

K

E

D

M

D

K

E

G

G

F

F

S

T

T

V

S

G

P

P

H

K

F

E

D

R

K

K

L

L

G

G

M

I

R

K

G

G

S

S

N

P

T

T

A

T

E

E

L

L

I

Y

F

F

E

E

D

N

V

C

E

R

V

I

P

P

F

G

E

D

N

R

V

I

L

I

G

G

E

E

E

P

G

G

R

T

G

G

V

F

A

K

V

T

L

A

M

L

S

A

G

T

L

L

D

D

Y

H

E
x
T

R

R

V

P

V

T

L

I

A

G

G

A

I

Q

G

A

T

V

G

G

I

I

M

A

A

Q

A

G

C

A

L

L

D

D

E

A

V

A

M

I

P

A

Y

Y

L

T

A

K

E

E

R
|
R

K

K

Q

Q

F
|
F

G

G

K

R

P

P

I
x
V

G

S

S

D

F
x
N

Q

Q

L

G

M

V

Q

Q

G

F

K

M

I

L

A

A

D

D

M

M

Y

A

T

M

A

K

M

I

N

E

S

A

A

A

R

R

A

L

Y

M

V

V

Y

Y

E

S

V

A

A

A

K

A

A

R

C

A

D

E

R

R

G

G

D

D

V

L

T

G

R

F

Q

I

D

S

A

A

A

S

C

K

C

C

L

-

Y

F

A

A

S

S

E

D

Q

V

A

A

M

M

V

E

Q

V

A

T

H

T

Q

D

A

A

V

V

Q
|
Q

A
x
L

L

F

G

G

G
|
G

A

Y

G

G

F

Y

L

T

S

Q

D

D

S

F

P

P

V

V

S

E

R

R

I

M

F

M

R

R

D

D

A

A

K

K

L
x
I

M

T

E

Q

I

I

G
x
Y

A
x
E

G

G

T
|
T

S

N

E
x
Q

I

I

R

Q

R

R

M

V

L

V

I

M

G

S

R
|
R

E

A

M

L

active site: L116 (≠ M130), S117 (= S131), T233 (≠ E247), E353 (≠ A368), R365 (= R380)
binding dihydroflavine-adenine dinucleotide: Y114 (≠ L128), L116 (≠ M130), S117 (= S131), G122 (= G136), S123 (= S137), W147 (= W161), I148 (= I162), T149 (= T163), R259 (= R273), F262 (= F276), V266 (≠ I280), N269 (≠ F283), Q326 (= Q341), L327 (≠ A342), G330 (= G345), I348 (≠ L363), Y352 (≠ G367), T355 (= T370), Q357 (≠ E372)

2vigB Crystal structure of human short-chain acyl coa dehydrogenase
39% identity, 97% coverage: 10:383/387 of query aligns to 1:367/371 of 2vigB

query
sites
2vigB

L

L

G

P

E

E

D

T

V

H

N

Q

A

M

L

L

R

L

E

Q

M

T

V

C

H

R

R

D

W

F

A

A

Q

E

E

K

R

E

V

L

K

F

P

P

M

I

A

A

E

Q

I

V

D

D

A

K

S

E

N

H

A

L

F

F

P

P

N

A

E

A

L

Q

W

V

K

K

E

K

M

M

G

G

D

G

L

L

G

G

L

L

G

A

V

M

T

D

V

V

P

P

E

E

Y

L

G

G

A

A

G

G

M

L

S

D

Y

Y

L

L

A

A

H

Y

T

A

I

I

A

A

V

M

E

E

V

I

A

S

R

R

A

G

S

C

A

A

S

S

V

T

S

G

L

V

S

I

Y

M

G

S

A

V

H

N

S

N

N

S

L

L

C

Y

V

L

N

G

Q

P

I

I

K

L

L

K

N

F

G

G

T

S

D

K

E

E

Q

Q

K

K

Q

Q

K

A

Y

W

L

V

P

T

R

P

L

F

I

T

S

S

G

G

D

D

H

K

V

I

G

G

A

C

L

F

A

A

M
x
L

S
|
S

E

E

S

P

S

G

A

N

G
|
G

S
|
S

D

D

V

A

V

G

S

A

M

A

K

S

L

T

R

T

A

A

E

R

K

A

R

E

N

G

D

D

H

S

Y

W

R

V

L

L

N

N

G

G

N

T

K

K

Y

A

W
|
W

I

I

T
|
T

N

N

G

A

P

W

D

E

A

A

D

S

T

A

L

A

V

V

Y

F

A

A

K

S

T

T

D

S

P

-

E

-

A

-

G

-

S

-

K

-

G

-

I

I

T

S

A

A

F

F

L

L

I

V

E

P

K

M

E

P

M

T

K

P

G

G

F

L

S

T

T

L

S

G

P

K

H

K

F

E

D

D

K

K

L

L

G

G

M

I

R

R

G

G

S

S

N

S

T

T

A

A

E

N

L

L

I

I

F

F

E

E

D

D

V

C

E

R

V

I

P

P

F

K

E

D

N

S

V

I

L

L

G

G

E

E

E

P

G

G

R

M

G

G

V
x
F

A

K

V

I

L

A

M
|
M

S
x
Q

G

T

L
|
L

D
|
D

Y

M

E
x
G

R
|
R

V

I

V

G

L

I

A

A

G

S

I

Q

G

A

T

L

G

G

I

I

M

A

A

Q

A

T

C

A

L

L

D

D

E

C

V

A

M

V

P

N

Y

Y

L

A

A

E

E

N

R
|
R

K

M

Q

A

F
|
F

G

G

K

A

P

P

I
x
L

G

T

S

K

F
x
L

Q

Q

L

V

M

I

Q

Q

G

F

K

K

I

L

A

A

D

D

M

M

Y

A

T

L

A

A

M

L

N

E

S

S

A

A

R

R

A

L

Y

L

V

T

Y

W

E

R

V

A

A

A

K

M

A

L

C

K

D

D

R

N

G

K

D

K

V

P

T

F

R

I

Q

K

D

E

A

A

A

M

C

A

C

K

L

L

Y

A

A

A

S

S

E

E

Q

A

A

A

M

T

V

A

Q

I

A

S

H

H

Q

Q

A

A

V

I

Q
|
Q

A
x
I

L

L

G

G

G
|
G

A

M

G

G

F

Y

L

V

S

T

D

E

S

M

P

P

V

A

S

E

R

R

I

H

F

Y

R

R

D

D

A

A

K

R

L
x
I

M

T

E

E

I

I

G
x
Y

A
x
E

G
|
G

T
|
T

S

S

E
|
E

I
|
I

R

Q

R

R

M

L

L

V

I

I

G

A

R
x
G

E

H

M

L

active site: L121 (≠ M130), S122 (= S131), G231 (≠ E247), E352 (≠ A368), G364 (≠ R380)
binding coenzyme a persulfide: S128 (= S137), F221 (≠ V237), M225 (= M241), Q226 (≠ S242), L228 (= L244), D229 (= D245), R232 (= R248), E352 (≠ A368), G353 (= G369), I357 (= I373)
binding flavin-adenine dinucleotide: L121 (≠ M130), S122 (= S131), G127 (= G136), S128 (= S137), W152 (= W161), T154 (= T163), R257 (= R273), F260 (= F276), L264 (≠ I280), L267 (≠ F283), Q325 (= Q341), I326 (≠ A342), G329 (= G345), I347 (≠ L363), Y351 (≠ G367), T354 (= T370), E356 (= E372)

4n5fA Crystal structure of a putative acyl-coa dehydrogenase with bound fadh2 from burkholderia cenocepacia j2315
38% identity, 96% coverage: 12:382/387 of query aligns to 8:378/378 of 4n5fA

query
sites
4n5fA

E

E

D

D

V

Q

N

R

A

M

L

I

R

R

E

D

M

A

V

A

H

R

R

A

W

F

A

A

Q

T

E

E

R

M

V

L

K

A

P

P

M

N

A

A

E

Q

I

W

D

D

A

H

S

D

N

A

A

H

F

L

P

P

N

D

E

A

L

I

W

V

K

A

E

Q

M

L

G

G

D

E

L

L

G

G

L

L

G

G

V

M

T

I

V

V

P

P

E

Q

E

E

Y

L

G

G

A

S

G

Y

M

T

S

D

Y

Y

L

V

A

A

H

Y

T

A

I

L

A

A

V

M

E

E

V

V

A

A

R

A

A

G

S

D

A

A

S

A

V

C

S

A

L

T

S

M

Y

M

G

S

A

V

H

H

S

N

N

S

L

V

C

G

V

C

N

G

Q

P

I

I

K

L

L

G

N

F

G

G

T

T

D

P

E

A

Q

Q

K

K

Q

D

K

R

Y

W

L

L

P

A

R

D

L

M

I

A

S

A

G

G

D

R

H

V

V

I

G

G

A

A

L

F

A

C

M
x
L

S
x
T

E

E

S

P

S

H

A

A

G
|
G

S
|
S

D

E

V

A

V

N

S

N

M

L

K

R

L

T

R

R

A

A

E

E

K

L

R

R

N

D

D

G

H

Q

Y

W

R

V

L

L

N

N

G

G

N

A

K

K

Y

Q

W
x
F

I

V

T
|
T

N

N

G

G

P

Q

D

R

A

A

D

G

T

V

L

A

V

I

V

V

Y

F

A

A

K

M

T

T

D

D

P

P

E

E

A

A

G

G

S

K

K

R

G

G

I

I

T

S

A

A

F

F

L

L

I

V

E

P

K

T

E

D

M

T

K

P

G

G

F

F

S

I

T

V

S

G

P

K

H

P

F

E

D

K

K

K

L

M

G

G

M

I

R

R

G

A

S

S

N

D

T
|
T

A

C

E

P

L

I

I

T

F

F

E

E

D

N

V

C

E

A

V

I

P

P

F

E

E

D

N

N

V

L

L

L

G

G

E

N

E

R

G

G

R

E

G

G

V

L

A

K

V

I

L

A

M

L

S

S

G

N

L

L

D

E

Y

G

E
x
G

R

R

V

I

V

G

L

I

A

A

G

A

I

Q

G

A

T

L

G

G

I

I

M

A

A

R

A

A

C

A

L

F

D

D

E

K

V

A

M

R

P

R

Y

Y

L

A

A

G

E

E

R

R

K

V

Q

Q

F

F

G

G

K

K

P

P

I

I

G

A

S

E

F

H

Q

Q

L

A

M

I

Q

Q

G

Q

K

K

I

L

A

A

D

D

M

M

Y

A

T

V

A

Q

M

I

N

N

S

A

A

A

R

R

A

L

Y

L

V

V

Y

H

E

H

V

A

A

A

K

K

A

L

C

R

D

T

R

A

G

G

D

L

V

P

T

C

R

L

Q

S

D

E

A

A

A

S

A

Q

C

A

C

K

L

L

Y

F

A

A

S

S

E

E

Q

M

A

A

M

E

V

R

Q

V

A

C

H

S

Q

D

A

A

V

I

Q

Q

A

I

L

H

G

G

A

Y

G

G

F

Y

L

L

S

V

D

D

S

Y

P

E

V

V

S

E

R

R

I

H

F

Y

R

R

D

D

A

A

K

R

L

I

M

T

E

Q

I

I

G
x
Y

A
x
E

G

G

T
|
T

S

S

E
|
E

I

V

R

Q

R

R

M
|
M

L

V

I

I

G

A

R
|
R

E

Q

M

L

active site: L126 (≠ M130), T127 (≠ S131), G243 (≠ E247), E364 (≠ A368), R376 (= R380)
binding dihydroflavine-adenine dinucleotide: L126 (≠ M130), T127 (≠ S131), G132 (= G136), S133 (= S137), F157 (≠ W161), T159 (= T163), T210 (= T214), Y363 (≠ G367), T366 (= T370), E368 (= E372), M372 (= M376)

3mpjB Structure of the glutaryl-coenzyme a dehydrogenase (see paper)
38% identity, 98% coverage: 6:383/387 of query aligns to 1:382/393 of 3mpjB

query
sites
3mpjB

M

M

Q

D

F

F

D

N

L

L

G

S

E

K

D

E

V

L

N

Q

A

M

L

L

R

Q

E

K

M

E

V

V

H

R

R

N

W

F

A

V

Q

N

E

K

R

K

V

I

K

V

P

P

M

F

A

A

A

D

E

Q

I

W

D

D

A

N

S

E

N

N

A
x
H

F
|
F

P

P

-
x
Y

N

E

E

E

L

A

W

V

K

R

E

P

M

M

G

G

D

E

L

L

G

G

L

F

G

G

V

T

T

V

V

I

P

P

E

E

Y

Y

G

G

A

E

G

G

M

M

S

D

-

Q

-

G

Y

W

L

L

A

A

H

A

T

M

I

I

A

V

V

T

E

E

V

I

A

A

R

R

A

G

S

S

A

S

S

A

V

L

S

R

L

V

S

Q

Y

L

G

N

A

M

H

E

S

V

N

L

L

G

C

C

V

A

N

Y

Q

T

I

I

K

L

L

T

N

Y

G

G

T

S

D

E

E

A

Q

L

K

K

Q

K

K

K

Y

Y

L

V

P

P

R

K

L

L

I

S

S

S

G

A

D

E

H

F

V

L

G

G

A

G

L
x
F

A

G

M
x
I

S
x
T

E

E

S

P

S

D

A

A

G
|
G

S
|
S

D

D

V

V

V

M

S

A

M

M

K

S

L

S

R

T

A

A

E

E

K

D

R

K

N

G

D

D

H

H

Y

W

R

L

L

L

N

N

G

G

N

S

K

K

Y

T

W
|
W

I
|
I

T
x
S

N

N

G

A

P
x
A

D
x
Q

A

A

D
|
D

T

V

L

L

V

I

V

Y

Y

Y

A

A

K

Y

T

T

D

D

P

K

E

A

A

A

G

G

S

S

K

R

G

G

I

L

T

S

A

A

F

F

L

V

I

I

E

E

-

P

K

R

E
x
N

M

F

K

P

G

G

F

I

S

K

T

T

S

S

P

-

H

N

F

L

D

E

K

K

L

L

G

G

M

S

R

H

G

A

S

S

N

P

T

T

A

G

E

E

L

L

I

F

F

L

E

D

D

N

V

V

E

K

V

V

P

P

F

K

E

E

N

N

V

I

L

L

G

G

E

K

E

P

G

G

R

D

G

G

V

A

A

R

V

I

L

V

M

F

S

G

G

S

L

L

D

N

Y

H

E
x
T

R

R

V

L

V

S

L

A

A

A

G

A

I

G

G

G

T

V

G

G

I

L

M

A

A

Q

A

A

C

C

L

L

D

D

E

A

V

A

M

I

P

K

Y

Y

L

C

A

N

E

E

R

R

K

R

Q

Q

F

F

G

G

K

K

P

P

I

I

G

G

S

D

F

F

Q

Q

L

M

M

N

Q

Q

G

D

K

M

I

I

A

A

D

Q

M

M

Y

A

T

V

A

E

M

V

N

E

S

A

A

A

R

R

A

L

Y

L

V

A

Y

Y

E

K

V

A

A

A

K

A

A

A

C

K

D

D

R

E

G

G

D

R

V

L

T

N

R

N

Q

G

D

L

A

D

A

V

A

A

C

M

C

A

L

K

Y

Y

A

A

S

A

E

G

Q

E

A

A

M

V

V

S

Q

K

-

C

A

A

H

N

Q

Y

A

A

V

M

Q

R

A

I

L

L

G

G

G

A

A

Y

G

G

F

Y

L

S

S

T

D

E

S

Y

P

P

V

V

S

A

R

R

I

F

F

Y

R

R

D

D

A

A

K

P

L

T

M

Y

E

Y

I

M

G
x
V

A
x
E

G

G

T
x
S

S

A

E
x
N

I

I

R

C

R

K

M
|
M

L

I

I

I

G

A

R
x
L

E

D

M

Q

M

L

active site: I128 (≠ M130), T129 (≠ S131), T245 (≠ E247), E367 (≠ A368), L379 (≠ R380)
binding flavin-adenine dinucleotide: F126 (≠ L128), I128 (≠ M130), T129 (≠ S131), G134 (= G136), S135 (= S137), W159 (= W161), I160 (= I162), S161 (≠ T163), V366 (≠ G367), S369 (≠ T370), N371 (≠ E372), M375 (= M376)
binding : H36 (≠ A41), F37 (= F42), Y39 (vs. gap), A164 (≠ P166), Q165 (≠ D167), D167 (= D169), N193 (≠ E194)

C3UVB0 Glutaryl-CoA dehydrogenase; GDH(Des); EC 1.3.99.32 from Desulfococcus multivorans (see paper)
38% identity, 98% coverage: 6:383/387 of query aligns to 1:382/389 of C3UVB0

query
sites
C3UVB0

M

M

Q

D

F

F

D

N

L

L

G

S

E

K

D

E

V

L

N

Q

A

M

L

L

R

Q

E

K

M

E

V

V

H

R

R

N

W

F

A

V

Q

N

E

K

R

K

V

I

K

V

P

P

M

F

A

A

A

D

E

Q

I

W

D

D

A

N

S

E

N

N

A

H

F

F

P

P

-

Y

N

E

E

E

L

A

W

V

K

R

E

P

M

M

G

G

D

E

L

L

G

G

L

F

G

G

V

T

T

V

V

I

P

P

E

E

Y

Y

G

G

A

E

G

G

M

M

S

D

-

Q

-

G

Y

W

L

L

A

A

H

A

T

M

I

I

A

V

V

T

E

E

V

I

A
|
A

R

R

A

G

S

S

A

S

S

A

V

L

S
x
R

L
x
V

S

Q

Y

L

G
x
N

A

M

H

E

S

V

N

L

L

G

C

C

V

A

N

Y

Q

T

I

I

K

L

L

T

N

Y

G

G

T

S

D

E

E

A

Q

L

K

K

Q

K

K

K

Y

Y

L

V

P

P

R

K

L

L

I

S

S

S

G

A

D

E

H

F

V

L

G

G

A

G

L
x
F

A
x
G

M
x
I

S
x
T

E

E

S

P

S

D

A

A

G

G

S
|
S

D

D

V

V

V

M

S

A

M

M

K

S

L

S

R

T

A

A

E

E

K

D

R

K

N

G

D

D

H

H

Y

W

R

L

L

L

N

N

G

G

N

S

K

K

Y

T

W
|
W

I
|
I

T
x
S

N

N

G

A

P

A

D

Q

A

A

D

D

T

V

L

L

V

I

V

Y

Y

Y

A

A

K

Y

T

T

D

D

P

K

E

A

A

A

G

G

S
|
S

K

R

G

G

I

L

T

S

A

A

F

F

L

V

I

I

E

E

-

P

K

R

E

N

M

F

K

P

G

G

F

I

S

K

T

T

S

S

P

-

H

N

F

L

D

E

K

K

L

L

G

G

M

S

R

H

G

A

S

S

N

P

T

T

A

G

E

E

L

L

I

F

F

L

E

D

D

N

V

V

E

K

V

V

P

P

F

K

E

E

N

N

V

I

L

L

G

G

E

K

E

P

G

G

R

D

G

G

V

A

A

R

V

I

L

V

M

F

S

G

G

S

L

L

D

N

Y

H

E

T

R

R

V

L

V

S

L

A

A

A

G

A

I

G

G

G

T

V

G

G

I

L

M

A

A

Q

A

A

C

C

L

L

D

D

E

A

V

A

M

I

P

K

Y

Y

L

C

A

N

E

E

R
|
R

K

R

Q

Q

F

F

G

G

K

K

P

P

I

I

G

G

S

D

F
|
F

Q
|
Q

L
x
M

M
x
N

Q

Q

G

D

K

M

I

I

A

A

D

Q

M

M

Y

A

T

V

A

E

M

V

N

E

S

A

A

A

R

R

A

L

Y

L

V

A

Y

Y

E

K

V

A

A

A

K

A

A

A

C

K

D

D

R

E

G

G

D

R

V

L

T

N

R

N

Q

G

D

L

A

D

A

V

A

A

C

M

C

A

L

K

Y

Y

A

A

S

A

E

G

Q

E

A

A

M

V

V

S

Q

K

-

C

A

A

H

N

Q

Y

A

A

V

M

Q
x
R

A

I

L

L

G

G

G
x
A

A

Y

G

G

F

Y

L

S

S

T

D

E

S

Y

P

P

V

V

S

A

R

R

I

F

F

Y

R

R

D

D

A

A

K

P

L

T

M

Y

E

Y

I

M

G
x
V

A
x
E

G
|
G

T
x
S

S
x
A

E
x
N

I

I

R

C

R

K

M

M

L

I

I

I

G

A

R

L

E

D

M

Q

M

L

A80 (= A82) mutation to E: Loses the FAD cofactor and dehydrogenase activity.
R87 (≠ S89) binding substrate
V88 (≠ L90) mutation to S: A residual dehydrogenase activity is observed.
N91 (≠ G93) binding substrate
FGIT 126:129 (≠ LAMS 128:131) binding FAD
S135 (= S137) binding FAD; binding substrate
WIS 159:161 (≠ WIT 161:163) binding FAD
S181 (= S183) binding substrate
R271 (= R273) binding FAD
FQMN 281:284 (≠ FQLM 283:286) binding FAD
R340 (≠ Q341) binding FAD
A344 (≠ G345) binding FAD
V366 (≠ G367) mutation to Y: Loses the FAD cofactor but a residual dehydrogenase activity is observed.
EGSAN 367:371 (≠ AGTSE 368:372) binding FAD

Sites not aligning to the query:

385 binding substrate

Query Sequence

>WP_010442787.1 NCBI__GCF_000192475.1:WP_010442787.1
MFMATMQFDLGEDVNALREMVHRWAQERVKPMAAEIDASNAFPNELWKEMGDLGLLGVTV
PEEYGGAGMSYLAHTIAVEEVARASASVSLSYGAHSNLCVNQIKLNGTDEQKQKYLPRLI
SGDHVGALAMSESSAGSDVVSMKLRAEKRNDHYRLNGNKYWITNGPDADTLVVYAKTDPE
AGSKGITAFLIEKEMKGFSTSPHFDKLGMRGSNTAELIFEDVEVPFENVLGEEGRGVAVL
MSGLDYERVVLAGIGTGIMAACLDEVMPYLAERKQFGKPIGSFQLMQGKIADMYTAMNSA
RAYVYEVAKACDRGDVTRQDAAACCLYASEQAMVQAHQAVQALGGAGFLSDSPVSRIFRD
AKLMEIGAGTSEIRRMLIGREMMNKML

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory