SitesBLAST
Comparing WP_010443333.1 NCBI__GCF_000192475.1:WP_010443333.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
3wy2A Crystal structure of alpha-glucosidase in complex with glucose (see paper)
61% identity, 90% coverage: 19:516/551 of query aligns to 4:501/535 of 3wy2A
- active site: D97 (= D112), R197 (= R212), D199 (= D214), E268 (= E283), H329 (= H346), D330 (= D347)
- binding beta-D-glucopyranose: D59 (= D74), Y62 (= Y77), H102 (= H117), R197 (= R212), D199 (= D214), E268 (= E283), H329 (= H346), D330 (= D347), R397 (= R411)
- binding glycerol: S101 (= S116), D169 (= D184), V170 (≠ L185), P175 (≠ K190), R178 (≠ Q193), Q179 (≠ D194), F203 (= F218), Y204 (= Y219), H206 (= H221), D240 (≠ S255), P244 (= P259), E280 (= E296)
- binding magnesium ion: D20 (= D35), R22 (≠ N37), V26 (≠ I41), D28 (= D43)
3wy1A Crystal structure of alpha-glucosidase (see paper)
61% identity, 90% coverage: 19:516/551 of query aligns to 4:501/535 of 3wy1A
- active site: D97 (= D112), R197 (= R212), D199 (= D214), E268 (= E283), H329 (= H346), D330 (= D347)
- binding glycerol: S101 (= S116), D169 (= D184), V170 (≠ L185), F203 (= F218), Y204 (= Y219), H206 (= H221)
- binding magnesium ion: D20 (= D35), D24 (= D39), V26 (≠ I41), D28 (= D43)
- binding (3R,5R,7R)-octane-1,3,5,7-tetracarboxylic acid: D59 (= D74), Y62 (= Y77), H102 (= H117), F144 (= F159), F163 (= F178), R197 (= R212), D199 (= D214), T200 (= T215), G225 (≠ I240), E268 (= E283), F294 (= F310), H329 (= H346), D330 (= D347), R397 (= R411)
3wy4A Crystal structure of alpha-glucosidase mutant e271q in complex with maltose (see paper)
61% identity, 90% coverage: 19:516/551 of query aligns to 4:501/535 of 3wy4A
- active site: D97 (= D112), R197 (= R212), D199 (= D214), Q268 (≠ E283), H329 (= H346), D330 (= D347)
- binding alpha-D-glucopyranose: D59 (= D74), Y62 (= Y77), H102 (= H117), I143 (= I158), F163 (= F178), D199 (= D214), T200 (= T215), G225 (≠ I240), Q268 (≠ E283), Q268 (≠ E283), F294 (= F310), H329 (= H346), D330 (= D347), R397 (= R411), R397 (= R411)
- binding glycerol: S101 (= S116), D169 (= D184), V170 (≠ L185), F203 (= F218), Y204 (= Y219), H206 (= H221), L224 (≠ S239), G225 (≠ I240), A226 (= A241), P227 (= P242), F294 (= F310), D330 (= D347), E374 (= E388), K395 (= K409), G396 (= G410), G399 (= G413)
- binding magnesium ion: D20 (= D35), D24 (= D39), V26 (≠ I41), D28 (= D43)
3wy4B Crystal structure of alpha-glucosidase mutant e271q in complex with maltose (see paper)
57% identity, 66% coverage: 19:383/551 of query aligns to 4:323/421 of 3wy4B
- active site: D75 (= D112), R175 (= R212), D177 (= D214), Q237 (≠ E283)
- binding glycerol: S79 (= S116), N140 (= N177), D147 (= D184), F181 (= F218), Y182 (= Y219), F183 (= F220), H184 (= H221), D209 (≠ S255)
2ze0A Alpha-glucosidase gsj (see paper)
40% identity, 89% coverage: 19:510/551 of query aligns to 4:486/531 of 2ze0A
O06994 Oligo-1,6-glucosidase 1; Dextrin 6-alpha-D-glucanohydrolase; Oligosaccharide alpha-1,6-glucosidase 1; Sucrase-isomaltase 1; Isomaltase 1; EC 3.2.1.10 from Bacillus subtilis (strain 168) (see paper)
36% identity, 92% coverage: 17:523/551 of query aligns to 2:534/561 of O06994
- D20 (= D35) binding Ca(2+)
- N22 (= N37) binding Ca(2+)
- D24 (= D39) binding Ca(2+)
- F26 (≠ I41) binding Ca(2+)
- D28 (= D43) binding Ca(2+)
4mazA The structure of mall mutant enzyme v200s from bacillus subtilus (see paper)
35% identity, 92% coverage: 18:523/551 of query aligns to 1:532/559 of 4mazA
7lv6B The structure of mall mutant enzyme s536r from bacillus subtilis
35% identity, 92% coverage: 18:523/551 of query aligns to 1:532/559 of 7lv6B
6k5pC Structure of mosquito-larvicidal binary toxin receptor, cqm1 (see paper)
39% identity, 91% coverage: 16:517/551 of query aligns to 1:493/531 of 6k5pC
- active site: D97 (= D112), R196 (= R212), D198 (= D214), E264 (≠ D286), H331 (= H346), D332 (= D347)
- binding calcium ion: N101 (≠ S116), D168 (= D184), H202 (≠ F218), M203 (≠ Y219), E205 (≠ H221)
- binding cobalt (ii) ion: H429 (= H459), H433 (= H463)
6k5pB Structure of mosquito-larvicidal binary toxin receptor, cqm1 (see paper)
39% identity, 91% coverage: 16:517/551 of query aligns to 1:493/531 of 6k5pB
- active site: D97 (= D112), R196 (= R212), D198 (= D214), E264 (≠ D286), H331 (= H346), D332 (= D347)
- binding calcium ion: N101 (≠ S116), D168 (= D184), H202 (≠ F218), M203 (≠ Y219), E205 (≠ H221)
- binding magnesium ion: H7 (≠ G22), A8 (≠ G23)
6k5pA Structure of mosquito-larvicidal binary toxin receptor, cqm1 (see paper)
39% identity, 91% coverage: 16:517/551 of query aligns to 1:493/531 of 6k5pA
4m56A The structure of wild-type mall from bacillus subtilis (see paper)
35% identity, 92% coverage: 18:523/551 of query aligns to 1:529/555 of 4m56A
- active site: D95 (= D112), R195 (= R212), D197 (= D214), E250 (≠ D286), H326 (= H346), D327 (= D347)
- binding D-glucose: D57 (= D74), Y60 (= Y77), H100 (= H117), F142 (= F159), D197 (= D214), E250 (≠ D286), D327 (= D347), R413 (= R411)
5wczA Crystal structure of wild-type mall from bacillus subtilis with ts analogue 1-deoxynojirimycin (see paper)
35% identity, 92% coverage: 18:523/551 of query aligns to 1:529/556 of 5wczA
- active site: D95 (= D112), R195 (= R212), D197 (= D214), E250 (≠ D286), H326 (= H346), D327 (= D347)
- binding 1-deoxynojirimycin: D57 (= D74), Y60 (= Y77), H100 (= H117), F161 (= F178), D197 (= D214), V198 (≠ T215), E250 (≠ D286), H326 (= H346), D327 (= D347), R413 (= R411)
4h8vA Crystal structure of the trehalulose synthase mutb in complex with trehalulose
36% identity, 92% coverage: 14:518/551 of query aligns to 1:521/557 of 4h8vA
- active site: D99 (= D112), R198 (= R212), D200 (= D214), E254 (≠ Y275), H326 (= H346), D327 (= D347)
- binding calcium ion: D22 (= D35), N24 (= N37), D26 (= D39), I28 (= I41), D30 (= D43)
- binding 1-O-alpha-D-glucopyranosyl-D-fructose: D61 (= D74), Y64 (= Y77), H104 (= H117), F164 (= F178), D200 (= D214), E254 (≠ Y275), F256 (≠ G277), R284 (≠ E314), R291 (vs. gap), H326 (= H346), D327 (= D347), E386 (= E403), R414 (= R411)
2pwdA Crystal structure of the trehalulose synthase mutb from pseudomonas mesoacidophila mx-45 complexed to the inhibitor deoxynojirmycin (see paper)
36% identity, 92% coverage: 14:518/551 of query aligns to 1:521/557 of 2pwdA
- active site: D99 (= D112), R198 (= R212), D200 (= D214), E254 (≠ Y275), H326 (= H346), D327 (= D347)
- binding calcium ion: D22 (= D35), N24 (= N37), D26 (= D39), I28 (= I41), D30 (= D43)
- binding 1-deoxynojirimycin: D61 (= D74), Y64 (= Y77), H104 (= H117), F164 (= F178), D200 (= D214), E254 (≠ Y275), H326 (= H346), D327 (= D347), R414 (= R411)
2pweA Crystal structure of the mutb e254q mutant in complex with the substrate sucrose (see paper)
36% identity, 91% coverage: 19:518/551 of query aligns to 5:520/556 of 2pweA
- active site: D98 (= D112), R197 (= R212), D199 (= D214), Q253 (≠ Y275), H325 (= H346), D326 (= D347)
- binding calcium ion: D21 (= D35), N23 (= N37), D25 (= D39), I27 (= I41), D29 (= D43)
- binding beta-D-fructofuranose: F163 (= F178), Q253 (≠ Y275), F255 (≠ G277), D326 (= D347), R413 (= R411)
- binding alpha-D-glucopyranose: D60 (= D74), Y63 (= Y77), H103 (= H117), F163 (= F178), D199 (= D214), Q253 (≠ Y275), H325 (= H346), D326 (= D347), R413 (= R411)
2pwgA Crystal structure of the trehalulose synthase mutb from pseudomonas mesoacidophila mx-45 complexed to the inhibitor castanospermine (see paper)
36% identity, 91% coverage: 19:518/551 of query aligns to 5:520/556 of 2pwgA
- active site: D98 (= D112), R197 (= R212), D199 (= D214), E253 (≠ Y275), H325 (= H346), D326 (= D347)
- binding calcium ion: D21 (= D35), N23 (= N37), D25 (= D39), I27 (= I41), D29 (= D43)
- binding castanospermine: D60 (= D74), Y63 (= Y77), H103 (= H117), F163 (= F178), D199 (= D214), E253 (≠ Y275), D326 (= D347), R413 (= R411)
7p01A Structure of the maltase baag2 from blastobotrys adeninivorans in complex with acarbose (see paper)
36% identity, 90% coverage: 19:512/551 of query aligns to 7:534/572 of 7p01A
- binding 4,6-dideoxy-4-{[(1S,4R,5S,6S)-4,5,6-trihydroxy-3-(hydroxymethyl)cyclohex-2-en-1-yl]amino}-alpha-D-glucopyranose: D62 (= D74), Y65 (= Y77), H105 (= H117), Y150 (≠ I158), F170 (= F178), D207 (= D214), T208 (= T215), E265 (≠ D286), F291 (= F310), H338 (= H346), D339 (= D347), R431 (= R411), T529 (≠ G507), D530 (= D508)
- binding calcium ion: D23 (= D35), N25 (= N37), D27 (= D39), I29 (= I41), D31 (= D43)
- binding alpha-D-glucopyranose: R224 (≠ L231), F291 (= F310), A295 (vs. gap), E300 (≠ A317), S349 (≠ L357), D350 (= D358), D350 (= D358), A351 (vs. gap), Q427 (vs. gap), K428 (vs. gap)
- binding 1-methylpyrrolidin-2-one: S19 (≠ R31), I29 (= I41), M63 (≠ F75), H74 (≠ D86), W454 (= W435)
2pwfA Crystal structure of the mutb d200a mutant in complex with glucose (see paper)
36% identity, 91% coverage: 19:518/551 of query aligns to 4:519/555 of 2pwfA
- active site: D97 (= D112), R196 (= R212), A198 (≠ D214), E252 (≠ Y275), H324 (= H346), D325 (= D347)
- binding beta-D-glucopyranose: D59 (= D74), Y62 (= Y77), H102 (= H117), F162 (= F178), R196 (= R212), A198 (≠ D214), E252 (≠ Y275), H324 (= H346), D325 (= D347), R412 (= R411)
- binding calcium ion: D20 (= D35), N22 (= N37), D24 (= D39), I26 (= I41), D28 (= D43)
4ha1A Mutb inactive double mutant d200a-d415n in complex with isomaltulose
36% identity, 91% coverage: 19:518/551 of query aligns to 5:520/556 of 4ha1A
- active site: D98 (= D112), R197 (= R212), A199 (≠ D214), E253 (≠ Y275), H325 (= H346), D326 (= D347)
- binding calcium ion: D21 (= D35), N23 (= N37), D25 (= D39), I27 (= I41), D29 (= D43)
- binding 6-O-alpha-D-glucopyranosyl-D-fructose: D60 (= D74), Y63 (= Y77), H103 (= H117), F163 (= F178), E253 (≠ Y275), R283 (≠ E314), H325 (= H346), D326 (= D347), E385 (= E403), R413 (= R411)
Query Sequence
>WP_010443333.1 NCBI__GCF_000192475.1:WP_010443333.1
MNVQTNATAFEATKAASDWWRGGVIYQIYPRSFQDSNGDGIGDLKGITQRLGYIASLGVD
AIWISPFFKSPMKDFGYDVSDYRDVDPMFGTMADFKELLDQAHAHGLKVMIDLVLSHTSD
QHPWFKESRASRENPKSDWYVWADPKPDGTPPNNWLSIFGGSAWQWDARRLQYYLHNFLT
SQPDLNFHCKDVQDALLDVARFWLDLGVDGFRLDTINFYFHDAQLRDNPGLPMDQRNASI
APMVNPYNHQDHLYSKSQPENIAFLERLRALTDEYEGRACLGEVGDAQRGLEIMGEYTSG
DKRMHMCYAFEFLEKRALTAAYAKQVFDQLEDKAGDAWPCWAFSNHDVQRHASRWGLDDA
AVRQHAVLMMCLRGSACLYQGEELGLPEADVAFEDLQDPYGIEFWPEFKGRDGCRTPMVW
VAQDEQSGFSAGRPWLPVSATQAERAVDRLEQDPASMLHHYRHAIALRHAHSALMSGKQT
GMSQIGSVLTFTREDESEQVFCAFNLGDDPVDVTLPEGDWKVIGEMLGGVRGEGTLMLNA
AQFCLLAREKR
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory