SitesBLAST
Comparing WP_010529735.1 NCBI__GCF_000224785.1:WP_010529735.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 8 hits to proteins with known functional sites (download)
8jejA Cryo-em structure of na-dithionite reduced membrane-bound fructose dehydrogenase from gluconobacter japonicus
27% identity, 96% coverage: 7:559/578 of query aligns to 5:536/540 of 8jejA
- binding fe3-s4 cluster: R203 (≠ N206), C214 (= C222), C215 (≠ Q223), G216 (≠ Y224), N217 (≠ C225), N218 (≠ G226), N219 (≠ F227), C220 (= C228), C224 (= C233), I226 (≠ Y235), M229 (≠ K238), S341 (≠ G352)
- binding flavin-adenine dinucleotide: I11 (≠ V13), G12 (= G14), G14 (= G16), I15 (≠ W17), C16 (≠ A18), D35 (≠ E37), A36 (≠ R38), G97 (≠ L91), K100 (≠ T94), G101 (= G95), G104 (= G98), T105 (≠ A99), T106 (≠ G100), H108 (= H102), W109 (= W103), A110 (≠ N104), S112 (≠ D106), M221 (≠ E229), A250 (≠ S261), V252 (= V263), A287 (≠ N302), N288 (= N303), E291 (vs. gap), N475 (≠ D498), H476 (= H499), N519 (= N542), T521 (= T544), M524 (≠ V547)
7w2jD Cryo-em structure of membrane-bound fructose dehydrogenase from gluconobacter japonicus
27% identity, 96% coverage: 7:559/578 of query aligns to 2:533/537 of 7w2jD
- binding fe3-s4 cluster: R200 (≠ N206), C211 (= C222), G213 (≠ Y224), N214 (≠ C225), N215 (≠ G226), C217 (= C228), C221 (= C233), I223 (≠ Y235), A225 (= A237), S338 (≠ G352)
- binding flavin-adenine dinucleotide: G9 (= G14), G11 (= G16), D32 (≠ E37), A33 (≠ R38), Y59 (= Y56), K97 (≠ T94), G101 (= G98), T102 (≠ A99), T103 (≠ G100), H105 (= H102), W106 (= W103), S109 (≠ D106), V249 (= V263), A284 (≠ N302), N285 (= N303), E288 (vs. gap), K291 (vs. gap), L292 (≠ R305), N472 (≠ D498), N516 (= N542), S517 (≠ P543), T518 (= T544)
- binding heme c: P209 (≠ T218), I223 (≠ Y235)
8grjB Crystal structure of gamma-alpha subunit complex from burkholderia cepacia fad glucose dehydrogenase in complex with gluconolactone (see paper)
25% identity, 81% coverage: 95:560/578 of query aligns to 90:529/531 of 8grjB
- binding fe3-s4 cluster: C204 (= C222), C205 (≠ Q223), G206 (≠ Y224), N207 (≠ C225), N209 (≠ F227), C210 (= C228), C214 (= C233), P331 (≠ A351)
- binding flavin-adenine dinucleotide: T94 (≠ A99), H97 (= H102), W98 (= W103), A99 (≠ N104), S101 (≠ D106), M211 (≠ E229), V242 (= V263), A277 (≠ S298), N278 (≠ Y299), E281 (≠ N302), I285 (≠ L306), N467 (≠ D498), N511 (= N542), T513 (= T544)
- binding D-glucono-1,5-lactone: M211 (≠ E229), E333 (≠ S353), H355 (≠ T380), N466 (= N497), N467 (≠ D498), H468 (= H499), N511 (= N542)
Sites not aligning to the query:
7qf8A Crystal structure of a bacterial pyranose 2-oxidase from pseudoarthrobacter siccitolerans (see paper)
25% identity, 53% coverage: 254:558/578 of query aligns to 218:483/494 of 7qf8A
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 11, 12, 14, 15, 16, 37, 38, 114, 119, 120, 124, 125, 127
7qvaA Crystal structure of a bacterial pyranose 2-oxidase in complex with mangiferin (see paper)
25% identity, 53% coverage: 254:558/578 of query aligns to 198:448/457 of 7qvaA
- binding flavin-adenine dinucleotide: L206 (≠ Y262), V207 (= V263), G241 (≠ T297), A242 (≠ S298), D243 (≠ Y299), R246 (≠ N302), L387 (≠ D498), H388 (= H499), N432 (= N542), T434 (= T544)
- binding Mangiferin: M299 (≠ K382), A385 (≠ Q496), S386 (≠ N497), H388 (= H499)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 11, 12, 14, 15, 16, 36, 37, 38, 97, 102, 103, 106, 107, 108, 110
- binding Mangiferin: 51, 73, 108
P0DXE4 C-glycoside 3-oxidase; EC 1.1.3.50 from Microbacterium sp. (see paper)
26% identity, 52% coverage: 257:558/578 of query aligns to 217:489/501 of P0DXE4
- H429 (= H499) mutation to A: Loss of activity.
7qfdA Crystal structure of a bacterial pyranose 2-oxidase complex with d- glucose (see paper)
31% identity, 23% coverage: 428:558/578 of query aligns to 323:449/458 of 7qfdA
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 11, 14, 15, 16, 36, 37, 38, 101, 106, 107, 111, 112, 114, 210, 211, 246, 250
- binding alpha-D-glucopyranose: 77, 112
9fl2B Crystal structure of oscillatoria princeps pyranose oxidase (see paper)
21% identity, 96% coverage: 9:560/578 of query aligns to 6:527/528 of 9fl2B
- binding flavin-adenine dinucleotide: G11 (= G14), G13 (= G16), P14 (≠ W17), V15 (≠ A18), D34 (≠ E37), A35 (≠ R38), V64 (≠ L66), A114 (≠ G93), F115 (≠ T94), A116 (≠ G95), G119 (= G98), M120 (≠ A99), H123 (= H102), W124 (= W103), T125 (≠ N104), A127 (≠ D106), V240 (= V263), A275 (≠ S298), A276 (≠ Y299), K279 (≠ N302), F370 (≠ I389), N509 (= N542), T511 (= T544)
Query Sequence
>WP_010529735.1 NCBI__GCF_000224785.1:WP_010529735.1
MVKKLDKVDVVTVGVGWAGGIIASEVAKAGVKVVGLERGDDRSTEDFQMVHDEYRYAIRY
ELMQDLSQETITFRNNLDERALPMRQFGAFLIGTGVGGAGVHWNGDTWFFAPYDFQIKTM
TDEKYGKNKLPEEYTLQDWGITYDELEPYYRKFEKMAGTSGEPNPLRPERTEEYPTPPMK
STPILDQYMEAAKNLGLHPFRQPSANISEQYTNPDGQTLNQCQYCGFCEKFGCEYGAKSD
PTVTVIPTAQKTGNFELRTNSYVTGITHDGNKATGVRYVDVQTGEEFEQPADVVALTSYT
LNNCRLLLQSNLGRPYDPSTGKGVIGKNYCYQITAIATGFFKDNFNAAMGAGSLGTTLDD
YNNDNFDHSDLDFIHGGSITMKQLGKRPINENAVPGDIPRWGKKFKQESIKWFNRSIPVT
SQGASMPHKNNYLSLDPTYKDSFGNPLLRMTYDFTEQDHALYEYITARCAEILEEMGAEV
VEPKELTDHFDIVPSQNDHITGGVIMGEDPETSALNNYLQMWDVDNVFVIGASAFAHNGG
YNPTGTVGALAYRAAEGILKYRKEKGQLVKRNKNGKLA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory