SitesBLAST

P69783 PTS system glucose-specific EIIA component; EIIA-Glc; EIII-Glc; Glucose-specific phosphotransferase enzyme IIA component from Escherichia coli (strain K12) (see 6 papers)
44% identity, 80% coverage: 7:138/164 of query aligns to 15:146/169 of P69783

query
sites
P69783

K

K

G

D

D

T

S

G

K

T

T

I

E

E

I

I

Y

I

T

A

P

P

V

L

N

S

G

G

E

E

I

I

V

V

Q

N

L

I

E

E

E

D

V

V

P

P

D

D

P

V

V

V

F

F

S

A

Q

E

K

K

M

I

M

V

G

G

D

D

G

G

V

I

A

A

V

I

K

K

P

P

A

T

E

G

G

N

K

K

I

M

Y

V

A

A

P

P

I

V

D

D

G

G

E

T

I

I

I

G

Q

K

I

I

P

F

E

E

T

T

K

N

H
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H

A

A

I

F

G

S

L

I

R

E

A

S

K

D

D

S

G

G

T

V

E

E

V

L

L

F

V

V

H
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H

I

F

G

G

L

I

E

D

T

T

V

V

S

E

L

L

E

K

G

G

K

E

G

G

F

F

T

K

A

R

S

I

V

A

S

E

I

E

G

G

D

Q

Q

R

V

V

S

K

I

V

G

G

Q

D

P

T

L

V

M

I

D

E

F

F

D

D

L

L

E

P

Y

L

I

L

R

E

N

E

N

K

A

A

S

K

S

S

D

T

M

L

T

T

P

P

I

V

V

V

I

I

T

S

N

N

A

M

Q

D

E

E

H76 (= H68) Important for phospho-donor activity; binding Zn(2+); mutation to Q: Unable to transfer phosphoryl group.
H91 (= H83) active site, Tele-phosphohistidine intermediate; for EIIA activity; binding Zn(2+); modified: Phosphohistidine; by HPr; mutation to Q: Unable to be phosphorylated by Hpr.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
4 F→W: Same activity as the wild-type.

1glcF Cation promoted association (cpa) of a regulatory and target protein is controlled by phosphorylation (see paper)
45% identity, 78% coverage: 11:138/164 of query aligns to 11:138/161 of 1glcF

query
sites
1glcF

S

S

K

T

T

I

E

E

I

I

Y

I

T

A

P

P

V

L

N

S

G

G

E

E

I

I

V

V

Q

N

L

I

E

E

E

D

V

V

P

P

D

D

P

V

V

V

F

F

S

A

Q

E

K

K

M

I

M

V

G

G

D

D

G

G

V

I

A

A

V

I

K

K

P

P

A

T

E

G

G

N

K

K

I

M

Y

V

A

A

P

P

I

V

D

D

G

G

E

T

I

I

I

G

Q

K

I

I

P

F

E

E

T

T

K

N

H
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H

A

A

I

F

G

S

L

I

R

E

A

S

K

D

D

S

G

G

T

V

E

E

V

L

L

F

V

V

H
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H

I

F

G

G

L

I

E

D

T

T

V

V

S

E

L

L

E

K

G

G

K

E

G

G

F

F

T

K

A

R

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V

A

S

E

I

E

G

G

D

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Q

R

V

V

S

K

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V

G

G

Q

D

P

T

L

V

M

I

D

E

F

F

D

D

L

L

E

P

Y

L

I

L

R

E

N

E

N

K

A

A

S

K

S

S

D

T

M

L

T

T

P

P

I

V

V

V

I

I

T

S

N

N

A

M

Q

D

E

E

binding zinc ion: H68 (= H68), H83 (= H83)

1o2fA Complex of enzyme iiaglc and iibglc phosphocarrier protein hpr from escherichia coli nmr, restrained regularized mean structure (see paper)
45% identity, 76% coverage: 14:138/164 of query aligns to 3:127/150 of 1o2fA

query
sites
1o2fA

E

E

I

I

Y

I

T

A

P

P

V

L

N

S

G

G

E

E

I

I

V

V

Q

N

L

I

E

E

E

D

V

V

P

P

D

D

P

V

V

V

F

F

S

A

Q

E

K

K

M

I

M

V

G

G

D

D

G

G

V

I

A

A

V

I

K

K

P

P

A

T

E

G

G

N

K

K

I

M

Y

V

A

A

P

P

I

V

D

D

G

G

E

T

I

I

I

G

Q

K

I

I

P

F

E

E

T
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T

K

N

H
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H

A

A

I

F

G

S

L

I

R

E

A

S

K

D

D

S

G

G

T

V

E

E

V

L

L

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V

V

H
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H

I

F

G

G

L

I

E
x
D

T

T

V

V

S

E

L

L

E

K

G

G

K

E

G

G

F

F

T

K

A

R

S

I

V

A

S

E

I

E

G

G

D

Q

Q

R

V

V

S

K

I

V

G

G

Q

D

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T

L

V

M

I

D

E

F

F

D

D

L

L

E

P

Y

L

I

L

R

E

N

E

N

K

A

A

S

K

S

S

D

T

M

L

T

T

P

P

I

V

V

V

I

I

T

S

N

N

A

M

Q

D

E

E

active site: T55 (= T66), H57 (= H68), H72 (= H83)
binding phosphite ion: H72 (= H83), D76 (≠ E87)

Query Sequence

>WP_010529793.1 NCBI__GCF_000224785.1:WP_010529793.1
MFKNLFKKGDSKTEIYTPVNGEIVQLEEVPDPVFSQKMMGDGVAVKPAEGKIYAPIDGEI
IQIPETKHAIGLRAKDGTEVLVHIGLETVSLEGKGFTASVSIGDQVSIGQPLMDFDLEYI
RNNASSDMTPIVITNAQETGKQYTMTEEKEGKASETVIITASDK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory