SitesBLAST

F136 (≠ L55) mutation to A: Decreases association with PtsG EIIB domain.
H247 (= H165) binding Zn(2+)
C257 (= C175) binding Zn(2+); mutation to A: Strongly reduced activity; when associated with A-259.; mutation to S: Strongly reduced activity; when associated with S-259.
C259 (= C177) binding Zn(2+); mutation to A: Strongly reduced activity; when associated with A-257.; mutation to S: Strongly reduced activity; when associated with S-257.
C264 (= C182) binding Zn(2+)
R306 (≠ A229) mutation to G: Forms dimers but not tetramers; when associated with G-310.
L310 (≠ P233) mutation to G: Forms dimers but not tetramers; when associated with G-306.

Sites not aligning to the query:

52 R→H: Shows increased expression and forms larger colonies.
86 H→R: Can be bound and inactivated by MtfA.

6jdbA Crystal structure of n-acetyl mannosmaine kinase in complex with mannac-6p and adp from haemophilus influenzae
30% identity, 97% coverage: 6:318/323 of query aligns to 4:289/290 of 6jdbA

query
sites
6jdbA

V

L

G

A

V

L

D

D

I

I

G

G

T

T

T
x
K

V

I

K

A

I

A

G

A

I

I

I

V

D

K

N

N

D

E

G

-

N

-

I

I

L

E

D

Q

K

R

W

Q

A

Q

I

I

H

H

T

T

N

P

R

R

T

E

N

N

S

-

G

-

E

-

F

-

I

V

V

V

S

E

D

G

I

M

W

H

K

Q

S

A

L

L

D

G

E

K

R

L

L

L

A

A

K

D

N

Y

A

-

I

-

P

-

K

E

K

G

T

Q

I

F

E

D

G

Y

I

V

G

A

I

V

G

A

A

S

P
x
T

G
|
G

F

I

I

I

D

N

N

N

D

G

T

I

G

L

Y

S

V
x
A

F
x
L

E
x
N

A

P

V

K

N
|
N

I

L

G

G

-

G

W

L

R

A

N

E

M

F

P

P

L

L

S

K

D

A

E

S

L

I

R

A

S

K

I

H

S

T

G

D

L

K

P

P

V

I

F

G

V

L

A

L

N
|
N

D
|
D

A

A

N

Q

A

A

V

T

M

Y

G

A

E

E

N

Y

W

Q

K

L

G

Q

A

N

G

F

G

E

N

Q

V

V

K

S

N

N

L

F

V

V

A

F

L

I

T

T

L

V

G
x
S

T
|
T

G

G

V

V

G

G

I

I

I

V

V

L

N

N

G

Q

D

I

I

L

L

Q

N

T

G

G

E

S

S

R

G

G

M

I

A

A

G

G

E
x
H

I

I

G

G

H
|
H

T

T

T

L

V

A

E

D

P

P

D

N

G

G

I

A

L

I

C
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C

N

G

C
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C

G

G

R

R

G

G

C
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C

L

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E
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E

T

A

I

I

A

A

S

S

A

G

T

R

G

A

M

I

V

-

R

-

Q

-

A

-

K

-

M

-

K

-

T

-

K

-

Q

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H

-

P

-

E

-

S

E

R

A

L

V

A

S

S

S

R

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L

W

E

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S

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C

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I

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T

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x
P

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|
K

D

E

I

V

F

F

E

E

L

R

A

F

D

R

A

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G

N

D

D

V

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C

A

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A

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Y

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A

A

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K

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I

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S

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K

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G
x
S

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V

S

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S

L

A

A

G

-

D

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Y

I

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K

A

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Y

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Y

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-

D

-

I

-

C

C

E

E

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A

A

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K

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F

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G

N

Q

D

D

A

A

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I

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I

G

G

A

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K

H

D

K

V

L

L

binding adenosine-5'-diphosphate: K12 (≠ T14), S129 (≠ G139), T130 (= T140), P195 (≠ A220), K196 (= K221), S241 (≠ G266)
binding 2-acetamido-2-deoxy-6-O-phosphono-alpha-D-mannopyranose: T62 (≠ P73), G63 (= G74), A72 (≠ V83), L73 (≠ F84), N74 (≠ E85), N77 (= N88), N102 (= N112), D103 (= D113), S129 (≠ G139), T130 (= T140), H152 (≠ E162), H155 (= H165), E174 (= E184)
binding zinc ion: H155 (= H165), C165 (= C175), C167 (= C177), C172 (= C182)

5f7qE Rok repressor lmo0178 from listeria monocytogenes bound to operator (see paper)
30% identity, 79% coverage: 67:321/323 of query aligns to 144:392/396 of 5f7qE

query
sites
5f7qE

G

G

I

V

G

G

I

I

G

A

A

I

P

S

G

G

F

L

I

V

D

N

N

R

D

K

T

K

G

G

Y

T

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V

F

I

E

R

A

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N

M

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G

W

W

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A

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A

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M

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A

-

H

I

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G

D

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I

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P

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V

F

Y

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A

D

N

K

D

N

A

I

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N

A

C

A

Y

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M

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G

A

E

E

N

L

W

W

K

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G

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A

E

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K

N

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N

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F

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A

A

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S

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G

G

T

A

G

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G

G

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I

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N

N

G

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Q

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N

Y

G

G

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A

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A

A

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H
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H

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G

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G

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C
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C

N

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C
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C

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C
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C

L

L

E

E

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M

I

Y

A

A

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S

A

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F

G

Y

M

F

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N

Q

R

A

G

K

E

M

E

K

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T

K

K

E

Q

A

H

Y

P

P

E

T

S

S

R

E

L

L

A

-

S

-

R

-

L

-

E

-

S

-

G

N

N

D

I

F

T

H

A

F

K

D

D

K

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F

A

E

K

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A

A

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A

A

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D

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M

C

A

R

T

E

E

I

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M

A

G

Y

K

T

M

A

G

D

E

V

Y

L

L

G

G

L

Y

A

G

I

I

S

R

N

N

A

I

S

I

T

N

L

T

I

F

N

N

P

P

S

E

K

K

V

V

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G

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G

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A

H

G

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D

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F

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T

L

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N

E

A

I

F

A

R

S

R

Q

Y

N

A

F

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F

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R

G

V

A

S

G

D

F

I

E

C

T

E

E

L

I

K

T

L

T

A

T

Q

S

L

L

G

E

N

D

D

P

A

A

G

W

I

L

I

Q

G

G

A

A

F

L

L

L

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V

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I

H

H

K

Q

L

L

H

F

N

Q

L

V

binding zinc ion: H243 (= H165), C253 (= C175), C255 (= C177), C260 (= C182)

Sites not aligning to the query:

binding : 5, 8, 12, 15, 32, 43, 44, 67, 68, 68, 69, 69, 70, 70, 71, 72, 73

Q93LQ8 Beta-glucoside kinase; EC 2.7.1.85 from Klebsiella pneumoniae (see paper)
30% identity, 95% coverage: 5:312/323 of query aligns to 3:283/297 of Q93LQ8

query
sites
Q93LQ8

I

I

V

A

G

A

V

F

D
|
D

I

I

G
|
G

G

G

T

T

T

A

V

L

K

K

I

M

G

G

I

V

I

M

D

A

N

R

D

D

G

G

N

R

I

L

L

L

D

E

K

-

W

-

A

-

I

-

H

-

T

T

N

A

R

R

T

Q

N

S

S

I

G

N

E

D

F

S

I

D

V

G

S

D

D

R

I

I

W

L

K

Q

S

A

L

M

D

L

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L

L

A

A

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A

N

H

A

-

I

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P

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K

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C

E

E

G

G

I

I

G

A

I

I

G

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A

A

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P

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G

F

Y

I

I

D

D

N

P

D

H

T

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G

Y

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M

A

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G

N

A

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A

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I

R

S

T

G

G

L

L

P

P

V

V

F

S

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V

A

E

N

N

D
|
D

A

A

N

N

A

C

A

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V

L

M

L

G

A

E

E

N

R

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W

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Q

G

G

A

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G

A

N

E

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A

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N

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L

L

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G

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G
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G

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G
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G

G

G

G

A

I

I

I

F

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C

N

Q

G

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Q

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N

G

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G

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A

A

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G

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E

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G

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T

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G

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L

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N

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H

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G

S

A

R

P

L

L

A

D

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S

R

-

L

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G

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N

-

I

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T

T

A

G

K

E

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F

E

D

L

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A

Y

D

D

A

A

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G

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D

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C

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R

I

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A

A

Y

E

T

F

A

F

D

N

V

G

L

L

G

G

L

H

A

G

I

L

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Y

N

N

A

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H

L

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N

D

P

P

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I

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G

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A

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G

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F

F

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T

L

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Y

F

A

G

L

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A

R

D

V

Y

S

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D

D

I

T

C

-

E

-

L

-

K

-

L

-

A

V

Q

S

L

H

G

G

N

N

D

D

A

A

G

G

I

L

I

I

G

G

A

A

A

V

F

Y

D7 (= D9) mutation to G: Loss of catalytic activity.
G9 (= G11) mutation to A: Loss of catalytic activity.
D103 (= D113) mutation to G: Loss of catalytic activity.
G131 (= G141) mutation to A: Loss of catalytic activity.
G133 (= G143) mutation to A: Loss of catalytic activity.

5f7rA Rok repressor lmo0178 from listeria monocytogenes bound to inducer (see paper)
26% identity, 97% coverage: 5:318/323 of query aligns to 3:305/306 of 5f7rA

query
sites
5f7rA

I

V

V

V

G

S

V

V

D
x
K

I

V

G

E

G
x
E

T

E

T

Q

V

L

K

L

I

F

G

A

I

L

I

T

D

D

N

L

D

N

G

A

N

E

I

I

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I

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K

N

W

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G

P

E

E

F

E

I

A

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I

W

A

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K

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N

L

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D

K

E

K

R

M

L

C

A

G

K

N

N

R

A

D

I

M

P

-

K

-

K

N

T

H

I

L

E

L

G

G

I

V

G

G

I

I

G

A

A

I

P

S

G
|
G

F

L

I

V

D

N

N

R

D

K

T

K

G

G

Y

T

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V

F

I

E

R

A

S

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M

I

L

G

G

W

W

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N

N

M

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A

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A

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M

L

L

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H

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A

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H

I

F

S

P

G

D

L

I

P

P

V

V

F

Y

V

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A

D

N

K

D
x
N

A

I

N

N

A

C

A

Y

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M

L

G

A

E

E

N

L

W

W

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L

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G

A

E

G

G

G

K

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S

K

N

N

N

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A

T

L

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T
x
S

L
x
V

G

G

T

A

G
|
G

V
x
L

G
|
G

G

L

G

S

I

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V

I

N

N

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A

A

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E
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E

I

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H
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H

T

T

V

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P

D

G

G

G

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C
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C

N

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C
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C

G

G

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G

G

C
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C

L

L

E
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E

T

M

I

Y

A

A

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S

A

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M

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A

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Y

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N

R

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L

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A

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L

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N

D

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A

F

K

D

D

K

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F

A

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K

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A

A

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A

A

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G

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D

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C

A

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E

I

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A

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K

T

M

A

G

D

E

V

Y

L

L

G

G

L

Y

A

G

I

I

S

R

N

N

A

I

S

I

T

N

L

T

I

F

N

N

P

P

S

E

K

K

V

V

L

I

I

I

G

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G

G

G
x
E

V

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A

H

G

R

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F

F

I

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A

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L

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C

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E

E

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S

L

L

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A

A

G
x
W

I

L

I

Q

G

G

A

A

F

L

L

L

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Q

L

L

binding alpha-D-glucopyranose: K7 (≠ D9), E10 (≠ G12), G70 (= G74), N110 (≠ D113), N110 (≠ D113), S134 (≠ T137), V135 (≠ L138), G138 (= G141), L139 (≠ V142), G140 (= G143), E159 (= E162), H162 (= H165), E181 (= E184), E253 (≠ G266), W293 (≠ G306)
binding zinc ion: H162 (= H165), C172 (= C175), C174 (= C177), C179 (= C182)

6jdcA Crystal structure of n-acetyl mannosmaine kinase in complex with mannac from haemophilus influenzae
30% identity, 97% coverage: 6:318/323 of query aligns to 4:269/269 of 6jdcA

query
sites
6jdcA

V

L

G

A

V

L

D

D

I

I

G

G

T

T

T

K

V

I

K

A

I

A

G

A

I

I

I

V

D

K

N

N

D

-

G

G

N

E

I

I

L

E

D

Q

K

R

W

Q

A

Q

I

I

H

H

T

T

N

P

R

R

T

E

N

N

S

-

G

-

E

-

F

-

I

V

V

V

S

E

D

G

I

M

W

H

K

Q

S

A

L

L

D

G

E

K

R

L

L

L

A

A

K

D

N

Y

A

-

I

-

P

-

K

E

K

G

T

Q

I

F

E

D

G

Y

I

V

G

A

I

V

G

A

A

S

P

T

G

G

F

I

I

I

D

N

N

N

D

G

T

I

G

L

Y

S

V

A

F

L

E

N

A

P

V

K

N

N

I

L

G

G

-

G

W

L

R

A

N

E

M

F

P

P

L

L

S

K

D

A

E

S

L

I

R

A

S

K

I

H

S

T

G

D

L

K

P

P

V

I

F

G

V

L

A

L

N

N

D

D

A

A

N

Q

A

A

V

T

M

Y

G

A

E

E

N

Y

W

Q

K

L

G

Q

A

N

G

F

G

E

N

Q

V

V

K

S

N

N

L

F

V

V

A

F

L

I

T

T

L

V

G

S

T

T

G

G

V

V

G

G

I

I

I

V

V

L

N

N

G

Q

D

I

I

L

L

Q

N

T

G

G

E

S

S

R

G

G

M

I

A

A

G

G

E

H

I

I

G

G

H
|
H

T

T

T

L

V

A

E

D

P

P

D

N

G

G

I

A

L

I

C
|
C

N

G

C
|
C

G

G

R

R

G

G

C
|
C

L

V

E

E

T

A

I

I

A

A

S

S

A

G

T

R

G

A

M

I

V

-

R

-

Q

-

A

-

K

-

M

-

K

-

T

-

K

-

Q

-

H

E

P

A

E

V

S

S

R

S

L

A

A

T

S

A

R

L

L

V

E

E

S

R

S

S

G

A

N

K

I

A

T

I

A

A

K

N

D

L

I

I

F

A

E

D

L

L

A

V

D

I

A

S

G

L

D

D

V

I

P

-

C

-

R

-

E

-

I

-

A

-

Y

-

T

-

A

-

D

-

V

-

L

-

G

-

L

-

A

-

I

-

S

-

N

-

A

-

S

-

T

-

L

-

I

-

N

-

P

-

S

Q

K

K

V

I

L

A

I

I

G

G

G

S

V

V

S

G

S

L

A

A

G

-

D

E

V

G

F

Y

I

L

K

S

L

L

I

V

E

E

N

K

A

Y

F

L

R

Q

R

D

Y

F

A

P

L

S

T

I

R

Y

V

C

S

-

D

-

I

-

C

C

E

E

L

I

K

E

L

T

A

A

Q

K

L

F

G

G

N

Q

D

D

A

A

G

G

I

L

I

I

G

G

A

A

F

Y

L

W

V

V

K

K

H

D

K

V

L

L

binding zinc ion: H156 (= H165), C166 (= C175), C168 (= C177), C173 (= C182)

Q91WG8 Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase; UDP-GlcNAc-2-epimerase/ManAc kinase; EC 3.2.1.183; EC 2.7.1.60 from Mus musculus (Mouse) (see paper)
32% identity, 88% coverage: 4:287/323 of query aligns to 408:688/722 of Q91WG8

query
sites
Q91WG8

S

S

I

A

V

L

G

A

V

V

D

D

I

L

G

G

T

T

T

N

V

L

K

R

I

V

G

A

I

I

I

V

D

S

N

M

D

K

G

G

N

E

I

I

L

V

D

K

K

K

W

Y

A

T

I

Q

H

F

T

N

N

P

R

K

T

T

N

Y

S

-

G

-

E

E

F

E

I

R

V

I

S

S

D

L

I

I

W

L

K

Q

S

M

L

C

D

V

E

E

R

A

L

A

A

A

K

E

N

A

A

V

I

K

P

L

K

N

K

C

T

R

I

I

E

L

G

G

I

V

G

G

I

I

G

S

A

T

P

G

G

G

F

R

I

V

D

N

N

P

D

Q

T

E

G

G

Y

V

V

V

F

L

E

H

A

S

V

T

N

K

I

L

-

I

-

Q

G

E

W

W

R

N

N

S

M

V

P

D

L

L

S

R

D

T

E

P

L

L

R

S

S

D

I

T

S

L

G

H

L

L

P

P

V

V

F

W

V

V

A

D

N

N

D

D

A

G

N

N

A

C

A

A

V

A

M

M

G

A

E

E

N

R

W

K

K

F

G

G

A

Q

G

G

G

K

N

G

V

Q

K

E

N

N

L

F

V

V

A

T

L

L

T

I

L

T

G

G

T

T

G

G

V

I

G

G

I

I

V

H

N

Q

G

H

D

E

I

L

L

I

N

H

G

G

E

S

S

S

G

F

M
x
C

A

A

G

A

E

E

I

L

G

G

H

H

T

L

T

V

V

V

E

S

P

L

D

D

G

G

I

P

L

D

C

C

N

S

C

C

G

G

R

S

R

H

G

G

C

C

L

I

E

E

T

A

I

Y

A

A

S

S

A

G

T

M

G

A

M

L

V

Q

R

R

Q

E

A

A

K

K

M

-

K

-

T

-

K

K

Q

L

H

H

P

D

E

E

S

D

R

L

L

L

A

L

S

V

R

E

-

G

-

M

-

S

L

V

E

P

S

K

S

D

G

E

N

A

I

V

T

G

A

A

K

L

D

H

I

L

F

I

E

Q

L

A

A

A

D

K

A

L

G

G

D

N

V

V

P

K

C

A

R

Q

E

S

I

I

I

L

A

R

Y

T

T

A

A

G

D

T

V

A

L

L

G

G

L

L

A

G

I

V

S

V

N

N

A

I

S

L

T

H

L

T

I

M

N

N

P

P

S

S

K

L

V

V

L

I

I

L

G

S

G

G

G

V

V

L

S

A

S

S

A

H

G

-

D

-

V

-

F

Y

I

I

K

H

L

I

I

V

E

K

N

D

A

V

F

I

R

R

R

Q

Y

Q

A

A

L

L

C563 (≠ M159) mutation to Y: Loss-of-function mutant resulting in impaired sialic acid biosynthesis.

Sites not aligning to the query:

704 P→R: Homozygous embryos exhibit cerebrospinal hemorrhages and defective angiogenesis in the diencephalon at 11 dpc, and become non-viable between 11.5 and 12.5 dpc. Loss-of-function mutant resulting in impaired sialic acid biosynthesis.

O35826 Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase; UDP-GlcNAc-2-epimerase/ManAc kinase; EC 3.2.1.183; EC 2.7.1.60 from Rattus norvegicus (Rat) (see paper)
32% identity, 88% coverage: 4:287/323 of query aligns to 408:688/722 of O35826

query
sites
O35826

S
|
S

I
x
A

V
x
L

G
x
A

V
|
V

D
|
D

I
x
L

G
|
G

T
|
T

T
x
N

V
x
L

K
x
R

I
x
V

G
x
A

I
|
I

I
x
V

D
x
S

N
x
M

D
x
K

G
|
G

N
x
E

I
|
I

L
x
V

D
x
K

K
|
K

W
x
Y

A
x
T

I
x
Q

H
x
F

T
x
N

N
x
P

R
x
K

T
|
T

N
x
Y

S

-

G

-

E
|
E

F
x
E

I
x
R

V
x
I

S
|
S

D
x
L

I
|
I

W
x
L

K
x
Q

S
x
M

L
x
C

D
x
V

E
|
E

R
x
A

L
x
A

A
|
A

K
x
E

N
x
A

A
x
V

I
x
K

P
x
L

K
x
N

K
x
C

T
x
R

I
|
I

E
x
L

G
|
G

I
x
V

G
|
G

I
|
I

G
x
S

A
x
T

P
x
G

G
|
G

F
x
R

I
x
V

D
x
N

N
x
P

D
x
Q

T
x
E

G
|
G

Y
x
V

V
|
V

F
x
L

E
x
H

A
x
S

V
x
T

N
x
K

I
x
L

-
x
I

-
x
Q

G
x
E

W
|
W

R
x
N

N
x
S

M
x
V

P
x
D

L
|
L

S
x
R

D
x
T

E
x
P

L
|
L

R
x
S

S
x
D

I
x
T

S
x
L

G
x
H

L
|
L

P
|
P

V
|
V

F
x
W

V
|
V

A
x
D

N
|
N

D
|
D

A
x
G

N
|
N

A
x
C

A
|
A

V
x
A

M
|
M

G
x
A

E
|
E

N
x
R

W
x
K

K
x
F

G
|
G

A
x
Q

G
|
G

G
x
K

N
x
G

V
x
Q

K
x
E

N
|
N

L
x
F

V
|
V

A
x
T

L
|
L

T
x
I

L
x
T

G
|
G

T
|
T

G
|
G

V
x
I

G
|
G

I
|
I

V
x
H

N
x
Q

G
x
H

D
x
E

I
x
L

L
x
I

N
x
H

G
|
G

E
x
S

S
|
S

G
x
F

M
x
C

A
|
A

G
x
A

E
|
E

I
x
L

G
|
G

H
|
H

T
x
L

T
x
V

V
|
V

E
x
S

P
x
L

D
|
D

G
|
G

I
x
P

L
x
D

C
|
C

N
x
S

C
|
C

G
|
G

R
x
S

R
x
H

G
|
G

C
|
C

L
x
I

E
|
E

T
x
A

I
x
Y

A
|
A

S
|
S

A
x
G

T
x
M

G
x
A

M
x
L

V
x
Q

R
|
R

Q
x
E

A
|
A

K
|
K

M

-

K

-

T

-

K
|
K

Q
x
L

H
|
H

P
x
D

E
|
E

S
x
D

R
x
L

L
|
L

A
x
L

S
x
V

R
x
E

-
x
G

-
x
M

-
x
S

L
x
V

E
x
P

S
x
K

S
x
D

G
x
E

N
x
A

I
x
V

T
x
G

A
|
A

K
x
L

D
x
H

I
x
L

F
x
I

E
x
Q

L
x
A

A
|
A

D
x
K

A
x
L

G
|
G

D
x
N

V
|
V

P
x
K

C
x
A

R
x
Q

E
x
S

I
|
I

I
x
L

A
x
R

Y
x
T

T
x
A

A
x
G

D
x
T

V
x
A

L
|
L

G
|
G

L
|
L

A
x
G

I
x
V

S
x
V

N
|
N

A
x
I

S
x
L

T
x
H

L
x
T

I
x
M

N
|
N

P
|
P

S
|
S

K
x
L

V
|
V

L
x
I

I
x
L

G
x
S

G
|
G

G
x
V

V
x
L

S
x
A

S
|
S

A
x
H

G

-

D

-

V

-

F
x
Y

I
|
I

K
x
H

L
x
I

I
x
V

E
x
R

N
x
D

A
x
V

F
x
I

R
|
R

R
x
Q

Y
x
Q

A
|
A

L
|
L

D413 (= D9) mutation D->K,N: No effect on UDP-N-acetylglucosamine 2-epimerase activity. Does not affect feedback inhibition by CMP-Neu5Ac. Loss of N-acylmannosamine kinase activity. Does not interfere with oligomerization.
R420 (≠ K16) mutation to M: No effect on UDP-N-acetylglucosamine 2-epimerase activity. Does not affect feedback inhibition by CMP-Neu5Ac. Loss of N-acylmannosamine kinase activity. Does not interfere with oligomerization.

Sites not aligning to the query:

1 UDP-N-acetylglucosamine 2-epimerase
49 H→A: Loss UDP-N-acetylglucosamine 2-epimerase activity. No effect on N-acylmannosamine kinase activity. Does not interfere with enzyme oligomerization.
110 H→A: Loss UDP-N-acetylglucosamine 2-epimerase activity. No effect on N-acylmannosamine kinase activity. Partial reduction of the dimerization process.
132 H→A: Loss UDP-N-acetylglucosamine 2-epimerase activity. No effect on N-acylmannosamine kinase activity. Partial reduction of the dimerization process.
155 H→A: Loss UDP-N-acetylglucosamine 2-epimerase activity. No effect on N-acylmannosamine kinase activity. Strong reduction of the dimerization process.
157 H→A: Loss UDP-N-acetylglucosamine 2-epimerase activity. No effect on N-acylmannosamine kinase activity. Strong reduction of the dimerization process.
406:722 N-acetylmannosamine kinase

2yi1A Crystal structure of n-acetylmannosamine kinase in complex with n- acetyl mannosamine 6-phosphate and adp. (see paper)
31% identity, 88% coverage: 4:287/323 of query aligns to 4:279/308 of 2yi1A

query
sites
2yi1A

S

S

I

A

V

L

G

A

V

V

D

D

I

L

G
|
G

T
|
T

T
x
N

V

L

K
x
R

I

V

G

A

I

I

I

V

D

S

N

M

D

K

G

G

N

E

I

I

L

V

D

K

K

K

W

Y

A

T

I

Q

H

F

T

N

N

P

R

K

T

T

N

Y

S

-

G

-

E

E

F

E

I

R

V

I

S

N

D

L

I

I

W

L

K

Q

S

M

L

C

D

V

E

E

R

A

L

A

A

A

K

E

N

A

A

V

I

K

P

L

K

N

K

C

T

R

I

I

E

L

G

G

I

V

G

G

I

I

G

S

A

T

P
x
G

G
|
G

F
x
R

I

V

D

N

N

P

D

R

T

E

G

G

Y

I

V

V

F

L

E

H

A
x
S

V
x
T

N

K

I
x
L

-

I

-

Q

G

E

W

W

R

N

N

S

M

V

P

D

L

L

S

R

D

T

E

P

L

L

R

S

S

D

I

T

S

L

G

H

L

L

P

P

V

V

F

W

V

V

A

D

N
|
N

D
|
D

A

G

N
|
N

A

C

A

A

V

A

M

L

G

A

E

E

N

R

W

K

K

F

G

G

A

Q

G

G

G

K

N

G

V

L

K

E

N

N

L

F

V

V

A

T

L

L

T

I

L

T

G
|
G

T
|
T

G
|
G

V
x
I

G

G

G
|
G

G

G

I

I

V

H

N

Q

G

H

D

E

I

L

L

I

N

H

G

G

E

S

S

S

G

F

M

C

A

A

G
x
A

E
|
E

I

L

G

G

H
|
H

T

L

T

V

V

V

E

S

P

L

D

D

G

G

I

P

L

D

C
|
C

N

S

C
|
C

G

G

R

S

R

H

G

G

C
|
C

L

I

E
|
E

T

A

I

Y

A

A

S

S

A
x
G

T

M

G

A

M

L

V

Q

R

R

Q

E

A

A

K

K

M

-

K

-

T

-

K

K

Q

L

H

H

P

D

E

E

S

D

R

L

L

L

A

L

S

-

R

-

L

V

E

E

S

G

S

M

G

S

N

A

I

V

T

G

A

A

K
x
L

D

H

I

L

F

I

E

Q

L

A

A

A

D

K

A

L

G

G

D

N

V

A

P

K

C

A

R

Q

E

S

I

I

I

L

A

R

Y

T

T

A

A

G

D

T

V

A

L

L

G

G

L

L

A

G

I

V

S

V

N

N

A

I

S

L

T

H

L

T

I

M

N

N

P

P

S

S

K

L

V

V

L

I

I

L

G

S

G

G

G
x
V

V

L

S

A

S

S

A

H

G

-

D

-

V

-

F

Y

I

I

K

H

L

I

I

V

E

K

N

D

A

V

F

I

R

R

R

Q

Y

Q

A

A

L

L

binding adenosine-5'-diphosphate: G11 (= G11), T13 (= T13), N14 (≠ T14), R16 (≠ K16), T140 (= T140), G189 (≠ A189), L216 (≠ K221), V261 (≠ G266)
binding 2-acetamido-2-deoxy-6-O-phosphono-alpha-D-mannopyranose: G12 (= G12), G71 (≠ P73), G72 (= G74), R73 (≠ F75), S84 (≠ A86), T85 (≠ V87), L87 (≠ I89), N112 (= N112), D113 (= D113), G139 (= G139), T140 (= T140), G141 (= G141), I142 (≠ V142), E162 (= E162), H165 (= H165), E184 (= E184)
bind