SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_010531662.1 NCBI__GCF_000224785.1:WP_010531662.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7qh2C Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
32% identity, 86% coverage: 49:466/485 of query aligns to 45:463/467 of 7qh2C

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binding flavin-adenine dinucleotide: V73 (≠ A77), G75 (= G79), S76 (≠ A80), G77 (= G81), T78 (= T82), G79 (= G83), L80 (= L84), A83 (≠ G87), C84 (≠ A88), P137 (= P141), G138 (≠ S142), E139 (≠ S143), A142 (= A146), T143 (= T148), G146 (= G151), N147 (= N152), S149 (≠ A154), T150 (≠ E155), A152 (≠ S157), G153 (= G158), E203 (= E208), G204 (= G209), I209 (≠ V214), E422 (= E425), H423 (= H426)
binding fe (iii) ion: H377 (= H381), H384 (= H388), E422 (= E425)

P9WIT1 Uncharacterized FAD-linked oxidoreductase Rv2280; EC 1.-.-.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
33% identity, 84% coverage: 59:467/485 of query aligns to 50:456/459 of P9WIT1

query
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P9WIT1

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K354 (≠ E360) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

8jdzA Crystal structure of mldhd in complex with 2-keto-3-methylvaleric acid (see paper)
29% identity, 86% coverage: 49:467/485 of query aligns to 40:454/454 of 8jdzA

query
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D

P

P

D

R

D

G

L

L

L

M

N
|
N

P

P

G

G

K

K

L

V

F

L

binding (3S)-3-methyl-2-oxopentanoic acid: R318 (= R332), W322 (≠ F336), H369 (= H381), H376 (= H388), H413 (= H426)
binding flavin-adenine dinucleotide: P68 (≠ A77), G70 (= G79), T71 (≠ A80), G72 (= G81), T73 (= T82), G74 (= G83), G78 (= G87), V79 (≠ A88), L90 (= L99), P132 (= P141), G133 (≠ S142), A134 (≠ S143), G140 (= G151), M141 (≠ N152), A143 (= A154), T144 (≠ E155), A146 (≠ S157), S147 (≠ G158), E200 (= E208), G201 (= G209), I206 (≠ V214), W322 (≠ F336), E412 (= E425), H413 (= H426), N449 (= N462)
binding manganese (ii) ion: H369 (= H381), H376 (= H388), E412 (= E425)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 32

8jdyA Crystal structure of mldhd in complex with 2-ketoisocaproic acid (see paper)
29% identity, 86% coverage: 49:467/485 of query aligns to 40:454/454 of 8jdyA

query
sites
8jdyA

P

P

R

D

A

A

V

V

F

W

V

P

R

Q

N

N

T

V

Q

D

H

Q

V

V

S

S

D

R

V

V

V

A

K

S

Q

L

L

C

N

Y

E

N

R

Q

E

G

I

V

P

P

Y

I

I

I

A
x
P

R

F

G
|
G

A
x
T

G
|
G

T
|
T

G
|
G

L

V

S

E

G

G

G
|
G

A
x
V

T

C

P

A

R

V

G

Q

G

G

E

G

V

V

I

C

I

I

S

N

L
|
L

V

T

R

H

M

M

K

D

Q

Q

L

I

L

T

H

E

L

L

D

N

L

T

E

E

N

D

R

F

Q

S

A

V

V

V

E

E

P

P

G

G

Y

V

I

T

N

R

L

K

K

A

L

L

T

N

Q

T

S

H

I

L

S

R

D

D

K

S

G

G

Y

L

Y

W

Y

F

A

P

P

V

D

D

P
|
P

S
x
G

S
x
A

Q

D

Y

-

A

-

C

A

T

S

I

L

G

C

G
|
G

N
x
M

V

A

A
|
A

E
x
T

N

G

S
x
A

G
x
S

G

G

A

T

H

N

C

A

L

V

K

R

Y

Y

G

G

V

T

T

M

T

R

N

D

H

N

I

V

L

I

G

N

L

L

E

E

V

V

L

L

P

P

S

D

G

G

E

R

I

L

E

H

I

T

-

A

-

G

-

R

G

G

K

R

S

H

G

Y

V

R

P

K

D

S

Q

A

P

A

G

G

Y

Y

D

N

L

L

L

T

G

G

L

L

L

F

T

V

G

G

S

S

E
|
E

G
|
G

T

T

L

L

G

G

I

I

V
x
I

T

T

K

S

I

T

T

T

V

L

N

R

I

L

L

H

K

P

N

A

P

P

E

E

G

A

K

T

K

V

T

A

V

A

L

T

A

C

Y

A

F

F

D

P

D

S

I

V

D

Q

D

A

A

A

S

V

Q

D

A

S

V

T

S

V

D

Q

I

I

I

L

A

Q

T

A

G

A

I

V

I

P

P

V

A

A

A

R

L

I

E

E

M

F

M

L

D

D

E

D

I

V

A

M

I

M

E

D

G

A

V

-

E

-

A

C

S

N

A

R

Y

H

P

S

V

L

G

N

H

C

P

P

S

-

D

-

I

V

A

A

A

P

F

T

L

L

L

F

I

L

E

E

V

F

D

H

G

G

I

S

Q

Q

A

Q

G

T

I

L

D

A

D

E

Q

Q

I

L

D

Q

D

R

I

T

L

E

T

A

V

I

C

T

K

Q

S

D

N

N

H

G

V

G

R

S

N

H

V

F

K

S

V

W

A

A

K

A

D

E

E

-

A

-

E

K

R

R

S

N

M

E

W

L

W

W

A

A

N

A

R
|
R

K

H

M

N

G

A

F
x
W

G

Y

A

A

M

A

G

L

A

A

I

L

S

S

P

P

-

G

-

S

D

K

Y

A

L

Y

V
x
S

Q

T

D

D

G

V

V

C

I

V

P

P

R

I

T

S

R

R

L

L

P

P

E

E

V

I

L

L

A

V

R

E

I

T

N

K

A

E

I

E

S

I

R

K

K

A

Y

S

E

K

L

L

R

T

I

G

A

A

N

I

I

V

F

G

H
|
H

A

V

G

G

D

D

G

G

N

N

L

F

H
|
H

P

C

L

I

I

L

L

L

F

V

D

D

A

P

S

D

V

-

P

-

G

A

E

E

S

E

D

Q

R

R

A

R

S

V

K

K

A

A

G

F

S

A

E

E

C

N

L

L

-

G

Q

R

V

R

C

A

A

L

D

A

V

L

G

G

S

T

I

C

T

T

G

G

E
|
E

H
|
H

G

G

V

I

G

G

I

L

E

G

K

K

S

R

A

Q

E

L

M

L

R

Q

F

E

I

E

F

V

S

G

D

P

E

V

E

G

M

V

A

E

A

T

Q

M

T

R

D

Q

I

L

R

K

H

N

V

T

F

L

N

D

P

P

D

R

D

G

L

L

L

M

N
|
N

P

P

G

G

K

K

L

V

F

L

binding 2-oxo-4-methylpentanoic acid: R318 (= R332), W322 (≠ F336), S336 (≠ V348), H369 (= H381), H376 (= H388), H413 (= H426)
binding flavin-adenine dinucleotide: P68 (≠ A77), G70 (= G79), T71 (≠ A80), G72 (= G81), T73 (= T82), G74 (= G83), G78 (= G87), V79 (≠ A88), L90 (= L99), P132 (= P141), G133 (≠ S142), A134 (≠ S143), G140 (= G151), M141 (≠ N152), A143 (= A154), T144 (≠ E155), A146 (≠ S157), S147 (≠ G158), E200 (= E208), G201 (= G209), I206 (≠ V214), E412 (= E425), N449 (= N462)
binding manganese (ii) ion: H369 (= H381), H376 (= H388), E412 (= E425)

8jdeA Crystal structure of mldhd in complex with d-lactate (see paper)
30% identity, 86% coverage: 49:467/485 of query aligns to 40:455/455 of 8jdeA

query
sites
8jdeA

P

P

R

D

A

A

V

V

F

W

V

P

R

Q

N

N

T

V

Q

D

H

Q

V

V

S

S

D

R

V

V

V

A

K

S

Q

L

L

C

N

Y

E

N

R

Q

E

G

I

V

P

P

Y

I

I

I

A
x
P

R

F

G
|
G

A
x
T

G
|
G

T
|
T

G
|
G

L

V

S

E

G

G

G
|
G

A
x
V

T

C

P

A

R

V

G

Q

G

G

E

G

V

V

I

C

I

I

S

N

L
|
L

V

T

R

H

M

M

K

D

Q

Q

L

I

L

T

H

E

L

L

D

N

L

T

E

E

N

D

R

F

Q

S

A

V

V

V

E

E

P

P

G

G

Y

V

I

T

N

R

L

K

K

A

L

L

T

N

Q

T

S

H

I

L

S

R

D

D

K

S

G

G

Y

L

Y

W

Y

F

A

P

P

V

D

D

P
|
P

S
x
G

S
x
A

Q

D

Y

-

A

-

C

A

T

S

I

L

G

C

G
|
G

N
x
M

V

A

A
|
A

E
x
T

N

G

S
x
A

G
x
S

G

G

A

T

H

N

C

A

L

V

K

R

Y

Y

G

G

V

T

T

M

T

R

N

D

H

N

I

V

L

I

G

N

L

L

E

E

V

V

L

L

P

P

S

D

G

G

E

R

I

L

E

H

I

T

-

A

-

G

-

R

G

G

K

R

S

H

G

Y

V

R

P

K

D

S

Q

A

P

A

G

G

Y

Y

D

N

L

L

L

T

G

G

L

L

L

F

T

V

G

G

S

S

E
|
E

G
|
G

T

T

L

L

G

G

I

I

V
x
I

T

T

K

S

I

T

T

T

V

L

N

R

I

L

L

H

K

P

N

A

P

P

E

E

G

A

K

T

K

V

T

A

V

A

L

T

A

C

Y

A

F

F

D

P

D

S

I

V

D

Q

D

A

A

A

S

V

Q

D

A

S

V

T

S

V

D

Q

I

I

I

L

A

Q

T

A

G

A

I

V

I

P

P

V

A

A

A

R

L

I

E

E

M

F

M

L

D

D

E

D

I

V

A

M

I

M

E

D

G

A

V

C

E

N

A

R

S

H

A

S

Y

K

P

-

V

L

G

N

H

C

P

P

S

-

D

-

I

V

A

A

A

P

F

T

L

L

L

F

I

L

E

E

V

F

D

H

G

G

I

S

Q

Q

A

Q

G

T

I

L

D

A

D

E

Q

Q

I

L

D

Q

D

R

I

T

L

E

T

A

V

I

C

T

K

Q

S

D

N

N

H

G

V

G

R

S

N

H

V

F

K

S

V

W

A

A

K

-

D

-

E

-

A

A

E

E

R

K

S

N

M

E

W

L

W

W

A

A

N

A

R
|
R

K

H

M

N

G

A

F
x
W

G

Y

A

A

M

A

G

L

A

A

I

L

S

S

P

P

-

G

-

S

D

K

Y

A

L

Y

V

S

Q

T

D

D

G

V

V

C

I

V

P

P

R

I

T

S

R

R

L

L

P

P

E

E

V

I

L

L

A

V

R

E

I

T

N

K

A

E

I

E

S

I

R

K

K

A

Y

S

E

K

L

L

R

T

I

G

A

A

N

I

I

V

F

G

H
|
H

A

V

G

G

D

D

G

G

N

N

L

F

H
|
H

P

C

L

I

I

L

L

L

F

V

D

D

A

P

S

D

V

-

P

D

G

A

E

E

S

E

D

Q

R

R

A

R

S

V

K

K

A

A

G

F

S

A

E

E

C

N

L

L

-

G

Q

R

V

R

C

A

A

L

D

A

V

L

G

G

S

T

I

C

T

T

G

G

E
|
E

H
|
H

G

G

V

I

G

G

I

L

E

G

K

K

S

R

A

Q

E

L

M

L

R

Q

F

E

I

E

F

V

S

G

D

P

E

V

E

G

M

V

A

E

A

T

Q

M

T

R

D

Q

I

L

R

K

H

N

V

T

F

L

N

D

P

P

D

R

D

G

L

L

L

M

N
|
N

P

P

G

G

K

K

L

V

F

L

binding flavin-adenine dinucleotide: P68 (≠ A77), G70 (= G79), T71 (≠ A80), G72 (= G81), T73 (= T82), G74 (= G83), G78 (= G87), V79 (≠ A88), L90 (= L99), P132 (= P141), G133 (≠ S142), A134 (≠ S143), G140 (= G151), M141 (≠ N152), A143 (= A154), T144 (≠ E155), A146 (≠ S157), S147 (≠ G158), E200 (= E208), G201 (= G209), I206 (≠ V214), W322 (≠ F336), E413 (= E425), H414 (= H426), N450 (= N462)
binding lactic acid: R318 (= R332), H369 (= H381), H376 (= H388), H414 (= H426)
binding manganese (ii) ion: H369 (= H381), H376 (= H388), E413 (= E425)

8jdxA Crystal structure of mldhd in complex with 2-ketoisovaleric acid (see paper)
29% identity, 86% coverage: 49:467/485 of query aligns to 40:455/455 of 8jdxA

query
sites
8jdxA

P

P

R

D

A

A

V

V

F

W

V

P

R

Q

N

N

T

V

Q

D

H

Q

V

V

S

S

D

R

V

V

V

A

K

S

Q

L

L

C

N

Y

E

N

R

Q

E

G

I

V

P

P

Y

I

I

I

A
x
P

R

F

G
|
G

A
x
T

G
|
G

T
|
T

G
|
G

L

V

S

E

G

G

G
|
G

A
x
V

T

C

P

A

R

V

G

Q

G

G

E

G

V

V

I

C

I

I

S

N

L
|
L

V

T

R

H

M

M

K

D

Q

Q

L

I

L

T

H

E

L

L

D

N

L

T

E

E

N

D

R

F

Q

S

A

V

V

V

E

E

P

P

G

G

Y

V

I

T

N

R

L

K

K

A

L

L

T

N

Q

T

S

H

I

L

S

R

D

D

K

S

G

G

Y

L

Y

W

Y

F

A

P

P

V

D

D

P
|
P

S
x
G

S
x
A

Q

D

Y

-

A

-

C

A

T

S

I

L

G

C

G
|
G

N
x
M

V

A

A
|
A

E
x
T

N

G

S
x
A

G
x
S

G

G

A

T

H

N

C

A

L

V

K

R

Y

Y

G

G

V

T

T

M

T

R

N

D

H

N

I

V

L

I

G

N

L

L

E

E

V

V

L

L

P

P

S

D

G

G

E

R

I

L

E

H

I

T

-

A

-

G

-

R

G

G

K

R

S

H

G

Y

V

R

P

K

D

S

Q

A

P

A

G

G

Y

Y

D

N

L

L

L

T

G

G

L

L

L

F

T

V

G

G

S

S

E
|
E

G
|
G

T

T

L

L

G

G

I

I

V
x
I

T

T

K

S

I

T

T

T

V

L

N

R

I

L

L

H

K

P

N

A

P

P

E

E

G

A

K

T

K

V

T

A

V

A

L

T

A

C

Y

A

F

F

D

P

D

S

I

V

D

Q

D

A

A

A

S

V

Q

D

A

S

V

T

S

V

D

Q

I

I

I

L

A

Q

T

A

G

A

I

V

I

P

P

V

A

A

A

R

L

I

E

E

M

F

M

L

D

D

E

D

I

V

A

M

I

M

E

D

G

A

V

-

E

-

A

C

S

N

A

R

Y

H

P

S

V

L

G

N

H

C

P

P

S

-

D

-

I

V

A

A

A

P

F

T

L

L

L

F

I

L

E

E

V

F

D

H

G

G

I

S

Q

Q

A

Q

G

T

I

L

D

A

D

E

Q

Q

I

L

D

Q

D

R

I

T

L

E

T

A

V

I

C

T

K

Q

S

D

N

N

H

G

V

G

R

S

N

H

V

F

K

S

V

W

A

A

K

A

D

E

E

K

A

-

E

-

R

R

S

N

M

E

W

L

W

W

A

A

N

A

R
|
R

K

H

M

N

G

A

F
x
W

G

Y

A

A

M

A

G

L

A

A

I

L

S

S

P

P

-

G

-

S

D

K

Y

A

L

Y

V

S

Q

T

D

D

G

V

V

C

I

V

P

P

R

I

T

S

R

R

L

L

P

P

E

E

V

I

L

L

A

V

R

E

I

T

N

K

A

E

I

E

S

I

R

K

K

A

Y

S

E

K

L

L

R

T

I

G

A

A

N

I

I

V

F

G

H
|
H

A

V

G

G

D

D

G

G

N

N

L

F

H
|
H

P

C

L

I

I

L

L

L

F

V

D

D

A

P

S

D

V

-

P

D

G

A

E

E

S

E

D

Q

R

R

A

R

S

V

K

K

A

A

G

F

S

A

E

E

C

N

L

L

-

G

Q

R

V

R

C

A

A

L

D

A

V

L

G

G

S

T

I

C

T

T

G

G

E
|
E

H
|
H

G

G

V

I

G

G

I

L

E

G

K

K

S

R

A

Q

E

L

M

L

R

Q

F

E

I

E

F

V

S

G

D

P

E

V

E

G

M

V

A

E

A

T

Q

M

T

R

D

Q

I

L

R

K

H

N

V

T

F

L

N

D

P

P

D

R

D

G

L

L

L

M

N
|
N

P

P

G

G

K

K

L

V

F

L

binding flavin-adenine dinucleotide: P68 (≠ A77), G70 (= G79), T71 (≠ A80), G72 (= G81), T73 (= T82), G74 (= G83), G78 (= G87), V79 (≠ A88), L90 (= L99), P132 (= P141), G133 (≠ S142), A134 (≠ S143), G140 (= G151), M141 (≠ N152), A143 (= A154), T144 (≠ E155), A146 (≠ S157), S147 (≠ G158), E200 (= E208), G201 (= G209), I206 (≠ V214), W322 (≠ F336), E413 (= E425), H414 (= H426), N450 (= N462)
binding 3-methyl-2-oxobutanoic acid: R318 (= R332), H369 (= H381), H376 (= H388), H414 (= H426)
binding manganese (ii) ion: H369 (= H381), H376 (= H388), E413 (= E425)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 32

8jdsA Crystal structure of mldhd in complex with pyruvate (see paper)
29% identity, 86% coverage: 49:467/485 of query aligns to 40:456/456 of 8jdsA

query
sites
8jdsA

P

P

R

D

A

A

V

V

F

W

V

P

R

Q

N

N

T

V

Q

D

H

Q

V

V

S

S

D

R

V

V

V

A

K

S

Q

L

L

C

N

Y

E

N

R

Q

E

G

I

V

P

P

Y

I

I

I

A
x
P

R

F

G
|
G

A
x
T

G
|
G

T
|
T

G
|
G

L

V

S

E

G

G

G
|
G

A
x
V

T

C

P

A

R

V

G

Q

G

G

E

G

V

V

I

C

I

I

S

N

L
|
L

V

T

R

H

M

M

K

D

Q

Q

L

I

L

T

H

E

L

L

D

N

L

T

E

E

N

D

R

F

Q

S

A

V

V

V

E

E

P

P

G

G

Y

V

I

T

N

R

L

K

K

A

L

L

T

N

Q

T

S

H

I

L

S

R

D

D

K

S

G

G

Y

L

Y

W

Y

F

A

P

P

V

D

D

P
|
P

S
x
G

S
x
A

Q

D

Y

-

A

-

C

A

T

S

I

L

G

C

G
|
G

N
x
M

V

A

A
|
A

E
x
T

N

G

S
x
A

G
x
S

G

G

A

T

H

N

C

A

L

V

K

R

Y

Y

G

G

V

T

T

M

T

R

N

D

H

N

I

V

L

I

G

N

L

L

E

E

V

V

L

L

P

P

S

D

G

G

E

R

I

L

E

H

I

T

-

A

-

G

-

R

G

G

K

R

S

H

G

Y

V

R

P

K

D

S

Q

A

P

A

G

G

Y

Y

D

N

L

L

L

T

G

G

L

L

L

F

T

V

G

G

S

S

E
|
E

G
|
G

T

T

L

L

G

G

I

I

V
x
I

T

T

K

S

I

T

T

T

V

L

N

R

I

L

L

H

K

P

N

A

P

P

E

E

G

A

K

T

K

V

T

A

V

A

L

T

A

C

Y

A

F

F

D

P

D

S

I

V

D

Q

D

A

A

A

S

V

Q

D

A

S

V

T

S

V

D

Q

I

I

I

L

A

Q

T

A

G

A

I

V

I

P

P

V

A

A

A

R

L

I

E

E

M

F

M

L

D

D

E

D

I

V

A

M

I

M

E

D

G

A

V

C

E

N

A

R

S

H

A

S

Y

K

P

-

V

L

G

N

H

C

P

P

S

-

D

-

I

V

A

A

A

P

F

T

L

L

L

F

I

L

E

E

V

F

D

H

G

G

I

S

Q

Q

A

Q

G

T

I

L

D

A

D

E

Q

Q

I

L

D

Q

D

R

I

T

L

E

T

A

V

I

C

T

K

Q

S

D

N

N

H

G

V

G

R

S

N

H

V

F

K

S

V

W

A

A

K

A

D

E

E

K

A

-

E

-

R

R

S

N

M

E

W

L

W

W

A

A

N

A

R
|
R

K

H

M

N

G

A

F
x
W

G

Y

A

A

M

A

G

L

A

A

I

L

S

S

P

P

-

G

-

S

D

K

Y

A

L

Y

V

S

Q

T

D

D

G

V

V

C

I

V

P

P

R

I

T

S

R

R

L

L

P

P

E

E

V

I

L

L

A

V

R

E

I

T

N

K

A

E

I

E

S

I

R

K

K

A

Y

S

E

K

L

L

R

T

I

G

A

A

N

I

I

V

F

G

H
|
H

A

V

G

G

D

D

G

G

N

N

L

F

H
|
H

P

C

L

I

I

L

L

L

F

V

D

D

A

P

S

D

V

-

P

D

G

A

E

E

S

E

D

Q

R

R

A

R

S

V

K

K

A

A

G

F

S

A

E

E

C

N

L

L

-

G

Q

R

V

R

C

A

A

L

D

A

V

L

G

G

S

T

I

C

T

T

G

G

E
|
E

H
|
H

G

G

V

I

G

G

I

L

E

G

K

K

S

R

A

Q

E

L

M

L

R

Q

F

E

I

E

F

V

S

G

D

P

E

V

E

G

M

V

A

E

A

T

Q

M

T

R

D

Q

I

L

R

K

H

N

V

T

F

L

N

D

P

P

D

R

D

G

L

L

L

M

N
|
N

P

P

G

G

K

K

L

V

F

L

binding flavin-adenine dinucleotide: P68 (≠ A77), G70 (= G79), T71 (≠ A80), G72 (= G81), T73 (= T82), G74 (= G83), G78 (= G87), V79 (≠ A88), L90 (= L99), P132 (= P141), G133 (≠ S142), A134 (≠ S143), G140 (= G151), M141 (≠ N152), A143 (= A154), T144 (≠ E155), A146 (≠ S157), S147 (≠ G158), E200 (= E208), G201 (= G209), I206 (≠ V214), W323 (≠ F336), E414 (= E425), H415 (= H426), N451 (= N462)
binding manganese (ii) ion: H370 (= H381), H377 (= H388), E414 (= E425)
binding pyruvic acid: R319 (= R332), H370 (= H381), H377 (= H388), H415 (= H426)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 32

8jdtA Crystal structure of mldhd in complex with 2-ketobutanoic acid (see paper)
29% identity, 86% coverage: 49:467/485 of query aligns to 40:455/455 of 8jdtA

query
sites
8jdtA

P

P

R

D

A

A

V

V

F

W

V

P

R

Q

N

N

T

V

Q

D

H

Q

V

V

S

S

D

R

V

V

V

A

K

S

Q

L

L

C

N

Y

E

N

R

Q

E

G

I

V

P

P

Y

I

I

I

A
x
P

R

F

G
|
G

A
x
T

G
|
G

T
|
T

G
|
G

L

V

S

E

G

G

G
|
G

A
x
V

T

C

P

A

R

V

G

Q

G

G

E

G

V

V

I

C

I

I

S

N

L
|
L

V

T

R

H

M

M

K

D

Q

Q

L

I

L

T

H

E

L

L

D

N

L

T

E

E

N

D

R

F

Q

S

A

V

V

V

E

E

P

P

G

G

Y

V

I

T

N

R

L

K

K

A

L

L

T

N

Q

T

S

H

I

L

S

R

D

D

K

S

G

G

Y

L

Y

W

Y

F

A

P

P

V

D

D

P
|
P

S
x
G

S
x
A

Q

D

Y

-

A

-

C

A

T

S

I

L

G

C

G
|
G

N
x
M

V

A

A
|
A

E
x
T

N

G

S
x
A

G
x
S

G

G

A

T

H

N

C

A

L

V

K

R

Y

Y

G

G

V

T

T

M

T

R

N

D

H

N

I

V

L

I

G

N

L

L

E

E

V

V

L

L

P

P

S

D

G

G

E

R

I

L

E

H

I

T

-

A

-

G

-

R

G

G

K

R

S

H

G

Y

V

R

P

K

D

S

Q

A

P

A

G

G

Y

Y

D

N

L

L

L

T

G

G

L

L

L

F

T

V

G

G

S

S

E
|
E

G
|
G

T

T

L

L

G

G

I

I

V
x
I

T

T

K

S

I

T

T

T

V

L

N

R

I

L

L

H

K

P

N

A

P

P

E

E

G

A

K

T

K

V

T

A

V

A

L

T

A

C

Y

A

F

F

D

P

D

S

I

V

D

Q

D

A

A

A

S

V

Q

D

A

S

V

T

S

V

D

Q

I

I

I

L

A

Q

T

A

G

A

I

V

I

P

P

V

A

A

A

R

L

I

E

E

M

F

M

L

D

D

E

D

I

V

A

M

I

M

E

D

G

A

V

C

E

N

A

R

S

H

A

S

Y

K

P

-

V

L

G

N

H

C

P

P

S

-

D

-

I

V

A

A

A

P

F

T

L

L

L

F

I

L

E

E

V

F

D

H

G

G

I

S

Q

Q

A

Q

G

T

I

L

D

A

D

E

Q

Q

I

L

D

Q

D

R

I

T

L

E

T

A

V

I

C

T

K

Q

S

D

N

N

H

G

V

G

R

S

N

H

V

F

K

S

V

W

A

A

K

E

D

-

E

-

A

-

E

K

R

R

S

N

M

E

W

L

W

W

A

A

N

A

R
|
R

K

H

M

N

G

A

F
x
W

G

Y

A

A

M

A

G

L

A

A

I

L

S

S

P

P

-

G

-

S

D

K

Y

A

L

Y

V

S

Q

T

D

D

G

V

V

C

I

V

P

P

R

I

T

S

R

R

L

L

P

P

E

E

V

I

L

L

A

V

R

E

I

T

N

K

A

E

I

E

S

I

R

K

K

A

Y

S

E

K

L

L

R

T

I

G

A

A

N

I

I

V

F

G

H
|
H

A

V

G

G

D

D

G

G

N

N

L

F

H
|
H

P

C

L

I

I

L

L

L

F

V

D

D

A

P

S

D

V

-

P

D

G

A

E

E

S

E

D

Q

R

R

A

R

S

V

K

K

A

A

G

F

S

A

E

E

C

N

L

L

-

G

Q

R

V

R

C

A

A

L

D

A

V

L

G

G

S

T

I

C

T

T

G

G

E
|
E

H
|
H

G

G

V

I

G

G

I

L

E

G

K

K

S

R

A

Q

E

L

M

L

R

Q

F

E

I

E

F

V

S

G

D

P

E

V

E

G

M

V

A

E

A

T

Q

M

T

R

D

Q

I

L

R

K

H

N

V

T

F

L

N

D

P

P

D

R

D

G

L

L

L

M

N
|
N

P

P

G

G

K

K

L

V

F

L

binding 2-ketobutyric acid: R318 (= R332), H369 (= H381), H376 (= H388), H414 (= H426)
binding flavin-adenine dinucleotide: P68 (≠ A77), G70 (= G79), T71 (≠ A80), G72 (= G81), T73 (= T82), G74 (= G83), G78 (= G87), V79 (≠ A88), L90 (= L99), P132 (= P141), G133 (≠ S142), A134 (≠ S143), G140 (= G151), M141 (≠ N152), A143 (= A154), T144 (≠ E155), A146 (≠ S157), S147 (≠ G158), E200 (= E208), G201 (= G209), I206 (≠ V214), W322 (≠ F336), E413 (= E425), H414 (= H426), N450 (= N462)
binding manganese (ii) ion: H369 (= H381), H376 (= H388), E413 (= E425)

8jdvA Crystal structure of mldhd in complex with 2-ketohexanoic acid (see paper)
29% identity, 86% coverage: 49:467/485 of query aligns to 40:454/454 of 8jdvA

query
sites
8jdvA

P

P

R

D

A

A

V

V

F

W

V

P

R

Q

N

N

T

V

Q

D

H

Q

V

V

S

S

D

R

V

V

V

A

K

S

Q

L

L

C

N

Y

E

N

R

Q

E

G

I

V

P

P

Y

I

I

I

A
x
P

R

F

G
|
G

A
x
T

G
|
G

T
|
T

G
|
G

L
x
V

S

E

G

G

G
|
G

A
x
V

T

C

P

A

R

V

G

Q

G

G

E

G

V

V

I

C

I

I

S

N

L
|
L

V

T

R

H

M

M

K

D

Q

Q

L

I

L

T

H

E

L

L

D

N

L

T

E

E

N

D

R

F

Q

S

A

V

V

V

E

E

P

P

G

G

Y

V

I

T

N

R

L

K

K

A

L

L

T

N

Q

T

S

H

I

L

S

R

D

D

K

S

G

G

Y

L

Y

W

Y

F

A

P

P

V

D

D

P
|
P

S
x
G

S
x
A

Q

D

Y

-

A

-

C

A

T

S

I

L

G

C

G
|
G

N
x
M

V

A

A
|
A

E
x
T

N

G

S
x
A

G
x
S

G

G

A

T

H

N

C

A

L

V

K

R

Y

Y

G

G

V

T

T

M

T

R

N

D

H

N

I

V

L

I

G

N

L

L

E

E

V

V

L

L

P

P

S

D

G

G

E

R

I

L

E

H

I

T

-

A

-

G

-

R

G

G

K

R

S

H

G

Y

V

R

P

K

D

S

Q

A

P

A

G

G

Y

Y

D

N

L

L

L

T

G

G

L

L

L

F

T

V

G

G

S

S

E
|
E

G
|
G

T

T

L

L

G

G

I

I

V
x
I

T

T

K

S

I

T

T

T

V

L

N

R

I

L

L

H

K

P

N

A

P

P

E

E

G

A

K

T

K

V

T

A

V

A

L

T

A

C

Y

A

F

F

D

P

D

S

I

V

D

Q

D

A

A

A

S

V

Q

D

A

S

V

T

S

V

D

Q

I

I

I

L

A

Q

T

A

G

A

I

V

I

P

P

V

A

A

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binding 2-Ketohexanoic acid: V75 (≠ L84), R317 (= R332), W321 (≠ F336), H368 (= H381), H375 (= H388), H413 (= H426)
binding flavin-adenine dinucleotide: P68 (≠ A77), G70 (= G79), T71 (≠ A80), G72 (= G81), T73 (= T82), G74 (= G83), G78 (= G87), V79 (≠ A88), L90 (= L99), P132 (= P141), G133 (≠ S142), A134 (≠ S143), G140 (= G151), M141 (≠ N152), A143 (= A154), T144 (≠ E155), A146 (≠ S157), S147 (≠ G158), E200 (= E208), G201 (= G209), I206 (≠ V214), W321 (≠ F336), Y322 (≠ G337), E412 (= E425), H413 (= H426), N449 (= N462)
binding manganese (ii) ion: H368 (= H381), H375 (= H388), E412 (= E425)

3pm9A Crystal structure of a putative dehydrogenase (rpa1076) from rhodopseudomonas palustris cga009 at 2.57 a resolution
28% identity, 93% coverage: 15:466/485 of query aligns to 7:464/465 of 3pm9A

query
sites
3pm9A

V

I

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A

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