SitesBLAST – Find functional sites

 

SitesBLAST

Comparing WP_010531775.1 NCBI__GCF_000224785.1:WP_010531775.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

1glcF Cation promoted association (cpa) of a regulatory and target protein is controlled by phosphorylation (see paper)
43% identity, 98% coverage: 5:164/164 of query aligns to 2:161/161 of 1glcF

query
sites
1glcF
L
 
L
F
 
F
K
 
D
K
 
K
-
 
L
K
 
K
E
 
S
E
 
L
I
 
V
K
 
S
E
 
T
I
 
I
A
 
E
V
 
I
H
 
I
S
 
A
A
 
P
V
 
L
T
 
S
G
 
G
E
 
E
F
 
I
I
 
V
P
 
N
L
 
I
E
 
E
D
 
D
V
 
V
P
 
P
D
 
D
P
 
V
V
 
V
F
 
F
S
 
A
E
 
E
K
 
K
M
 
I
M
 
V
G
 
G
D
 
D
G
 
G
C
 
I
A
 
A
I
 
I
K
 
K
P
 
P
S
 
T
D
 
G
E
 
N
E
 
K
V
 
M
F
 
V
A
 
A
P
 
P
V
 
V
S
 
D
G
 
G
E
 
T
I
 
I
V
 
G
Q
 
K
V
 
I
F
 
F
P
 
E
T
 
T
K
 
N
H
|
H
A
 
A
I
 
F
G
 
S
I
 
I
E
 
E
A
 
S
E
 
D
N
 
S
G
 
G
A
 
V
Q
 
E
I
 
L
L
 
F
V
 
V
H
|
H
V
 
F
G
 
G
L
 
I
E
 
D
T
 
T
V
 
V
N
 
E
L
 
L
N
 
K
G
 
G
E
 
E
G
 
G
F
 
F
T
 
K
V
 
R
H
 
I
V
 
A
E
 
E
E
 
E
G
 
G
D
 
Q
T
 
R
V
 
V
N
 
K
Q
 
V
G
 
G
D
 
D
K
 
T
L
 
V
L
 
I
R
 
E
F
 
F
D
 
D
M
 
L
N
 
P
V
 
L
I
 
L
E
 
E
D
 
E
K
 
K
A
 
A
A
 
K
S
 
S
T
 
T
I
 
L
I
 
T
P
 
P
I
 
V
I
 
V
V
 
I
T
 
S
N
 
N
T
 
M
D
 
D
E
 
E
M
 
I
Q
 
K
E
 
E
I
 
L
R
 
I
P
 
-
N
 
K
G
 
L
A
 
S
G
 
G
S
 
S
V
 
V
T
 
T
A
 
V
G
 
G
Q
 
E
D
 
T
D
 
P
I
 
V
L
 
I
F
 
R
F
 
I
K
 
K
K
 
K

P69783 PTS system glucose-specific EIIA component; EIIA-Glc; EIII-Glc; Glucose-specific phosphotransferase enzyme IIA component from Escherichia coli (strain K12) (see 6 papers)
43% identity, 95% coverage: 9:164/164 of query aligns to 15:169/169 of P69783

query
sites
P69783
K
 
K
E
 
K
E
 
D
I
 
T
K
 
G
E
 
T
I
 
I
A
 
E
V
 
I
H
 
I
S
 
A
A
 
P
V
 
L
T
 
S
G
 
G
E
 
E
F
 
I
I
 
V
P
 
N
L
 
I
E
 
E
D
 
D
V
 
V
P
 
P
D
 
D
P
 
V
V
 
V
F
 
F
S
 
A
E
 
E
K
 
K
M
 
I
M
 
V
G
 
G
D
 
D
G
 
G
C
 
I
A
 
A
I
 
I
K
 
K
P
 
P
S
 
T
D
 
G
E
 
N
E
 
K
V
 
M
F
 
V
A
 
A
P
 
P
V
 
V
S
 
D
G
 
G
E
 
T
I
 
I
V
 
G
Q
 
K
V
 
I
F
 
F
P
 
E
T
 
T
K
 
N
H
|
H
A
 
A
I
 
F
G
 
S
I
 
I
E
 
E
A
 
S
E
 
D
N
 
S
G
 
G
A
 
V
Q
 
E
I
 
L
L
 
F
V
 
V
H
|
H
V
 
F
G
 
G
L
 
I
E
 
D
T
 
T
V
 
V
N
 
E
L
 
L
N
 
K
G
 
G
E
 
E
G
 
G
F
 
F
T
 
K
V
 
R
H
 
I
V
 
A
E
 
E
E
 
E
G
 
G
D
 
Q
T
 
R
V
 
V
N
 
K
Q
 
V
G
 
G
D
 
D
K
 
T
L
 
V
L
 
I
R
 
E
F
 
F
D
 
D
M
 
L
N
 
P
V
 
L
I
 
L
E
 
E
D
 
E
K
 
K
A
 
A
A
 
K
S
 
S
T
 
T
I
 
L
I
 
T
P
 
P
I
 
V
I
 
V
V
 
I
T
 
S
N
 
N
T
 
M
D
 
D
E
 
E
M
 
I
Q
 
K
E
 
E
I
 
L
R
 
I
P
 
-
N
 
K
G
 
L
A
 
S
G
 
G
S
 
S
V
 
V
T
 
T
A
 
V
G
 
G
Q
 
E
D
 
T
D
 
P
I
 
V
L
 
I
F
 
R
F
 
I
K
 
K
K
 
K

Sites not aligning to the query:

1o2fA Complex of enzyme iiaglc and iibglc phosphocarrier protein hpr from escherichia coli nmr, restrained regularized mean structure (see paper)
44% identity, 91% coverage: 15:164/164 of query aligns to 2:150/150 of 1o2fA

query
sites
1o2fA
I
 
I
A
 
E
V
 
I
H
 
I
S
 
A
A
 
P
V
 
L
T
 
S
G
 
G
E
 
E
F
 
I
I
 
V
P
 
N
L
 
I
E
 
E
D
 
D
V
 
V
P
 
P
D
 
D
P
 
V
V
 
V
F
 
F
S
 
A
E
 
E
K
 
K
M
 
I
M
 
V
G
 
G
D
 
D
G
 
G
C
 
I
A
 
A
I
 
I
K
 
K
P
 
P
S
 
T
D
 
G
E
 
N
E
 
K
V
 
M
F
 
V
A
 
A
P
 
P
V
 
V
S
 
D
G
 
G
E
 
T
I
 
I
V
 
G
Q
 
K
V
 
I
F
 
F
P
 
E
T
|
T
K
 
N
H
|
H
A
 
A
I
 
F
G
 
S
I
 
I
E
 
E
A
 
S
E
 
D
N
 
S
G
 
G
A
 
V
Q
 
E
I
 
L
L
 
F
V
 
V
H
|
H
V
 
F
G
 
G
L
 
I
E
x
D
T
 
T
V
 
V
N
 
E
L
 
L
N
 
K
G
 
G
E
 
E
G
 
G
F
 
F
T
 
K
V
 
R
H
 
I
V
 
A
E
 
E
E
 
E
G
 
G
D
 
Q
T
 
R
V
 
V
N
 
K
Q
 
V
G
 
G
D
 
D
K
 
T
L
 
V
L
 
I
R
 
E
F
 
F
D
 
D
M
 
L
N
 
P
V
 
L
I
 
L
E
 
E
D
 
E
K
 
K
A
 
A
A
 
K
S
 
S
T
 
T
I
 
L
I
 
T
P
 
P
I
 
V
I
 
V
V
 
I
T
 
S
N
 
N
T
 
M
D
 
D
E
 
E
M
 
I
Q
 
K
E
 
E
I
 
L
R
 
I
P
 
-
N
 
K
G
 
L
A
 
S
G
 
G
S
 
S
V
 
V
T
 
T
A
 
V
G
 
G
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E
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T
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P
I
 
V
L
 
I
F
 
R
F
 
I
K
 
K
K
 
K

P09323 PTS system N-acetylglucosamine-specific EIICBA component; EIICBA-Nag; EII-Nag; EC 2.7.1.193 from Escherichia coli (strain K12) (see paper)
40% identity, 89% coverage: 19:164/164 of query aligns to 503:648/648 of P09323

query
sites
P09323
S
 
S
A
 
P
V
 
I
T
 
T
G
 
G
E
 
D
F
 
V
I
 
V
P
 
A
L
 
L
E
 
D
D
 
Q
V
 
V
P
 
P
D
 
D
P
 
E
V
 
A
F
 
F
S
 
A
E
 
S
K
 
K
M
 
A
M
 
V
G
 
G
D
 
D
G
 
G
C
 
V
A
 
A
I
 
V
K
 
K
P
 
P
S
 
T
D
 
D
E
 
K
E
 
I
V
 
V
F
 
V
A
 
S
P
 
P
V
 
A
S
 
A
G
 
G
E
 
T
I
 
I
V
 
V
Q
 
K
V
 
I
F
 
F
P
 
N
T
 
T
K
 
N
H
 
H
A
 
A
I
 
F
G
 
C
I
 
L
E
 
E
A
 
T
E
 
E
N
 
K
G
 
G
A
 
A
Q
 
E
I
 
I
L
 
V
V
 
V
H
 
H
V
 
M
G
 
G
L
 
I
E
 
D
T
 
T
V
 
V
N
 
A
L
 
L
N
 
E
G
 
G
E
 
K
G
 
G
F
 
F
T
 
K
V
 
R
H
 
L
V
 
V
E
 
E
E
 
E
G
 
G
D
 
A
T
 
Q
V
 
V
N
 
S
Q
 
A
G
 
G
D
 
Q
K
 
P
L
 
I
L
 
L
R
 
E
F
 
M
D
 
D
M
 
L
N
 
D
V
 
Y
I
 
L
E
 
N
D
 
A
K
 
N
A
 
A
A
 
R
S
 
S
T
 
M
I
 
I
I
 
S
P
 
P
I
 
V
I
 
V
V
 
C
T
 
S
N
 
N
T
 
I
D
 
D
E
 
D
M
 
F
Q
 
S
E
 
G
I
 
L
R
 
I
P
 
I
N
 
K
G
 
A
A
 
Q
G
 
G
S
 
H
V
 
I
T
 
V
A
 
A
G
 
G
Q
 
Q
D
 
T
D
 
P
I
 
L
L
 
Y
F
 
E
F
 
I
K
 
K
K
 
K

Sites not aligning to the query:

Query Sequence

>WP_010531775.1 NCBI__GCF_000224785.1:WP_010531775.1
MFKNLFKKKEEIKEIAVHSAVTGEFIPLEDVPDPVFSEKMMGDGCAIKPSDEEVFAPVSG
EIVQVFPTKHAIGIEAENGAQILVHVGLETVNLNGEGFTVHVEEGDTVNQGDKLLRFDMN
VIEDKAASTIIPIIVTNTDEMQEIRPNGAGSVTAGQDDILFFKK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory