SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_010531824.1 NCBI__GCF_000224785.1:WP_010531824.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

A0A0P0ZBS7 o-succinylbenzoate synthase; OSB synthase; OSBS; 4-(2'-carboxyphenyl)-4-oxybutyric acid synthase; N-acylamino acid racemase; NAAAR; N-succinylamino acid racemase; NSAAR; NSAR; o-succinylbenzoic acid synthase; EC 4.2.1.113; EC 5.1.1.- from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see paper)
62% identity, 99% coverage: 4:368/370 of query aligns to 5:369/375 of A0A0P0ZBS7

query
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A0A0P0ZBS7

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K166 (= K165) mutation to A: Almost loss of activity with N-formyl-D/L-methionine.
D191 (= D190) mutation to A: Almost loss of activity with N-formyl-D/L-methionine.
E216 (= E215) mutation to A: Almost loss of activity with N-formyl-D/L-methionine.
D241 (= D240) mutation to A: Almost loss of activity with N-formyl-D/L-methionine.
K265 (= K264) mutation to A: Almost loss of activity with N-formyl-D/L-methionine.

1wufA Crystal structure of protein gi:16801725, member of enolase superfamily from listeria innocua clip11262 (see paper)
44% identity, 97% coverage: 5:362/370 of query aligns to 5:362/371 of 1wufA

query
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1wufA

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active site: F20 (= F20), L54 (≠ Y54), K163 (= K163), K165 (= K165), D190 (= D190), E215 (= E215), D240 (= D240), K264 (= K264), G291 (= G291), D317 (= D317), I318 (≠ T318)
binding magnesium ion: E215 (= E215), D240 (= D240), E241 (= E241)

Q9RYA6 N-succinylamino acid racemase; NSAAR; NSAR; N-acylamino acid racemase; NAAAR; o-succinylbenzoate synthase; OSB synthase; OSBS; EC 5.1.1.-; EC 5.1.1.-; EC 4.2.1.113 from Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / CCUG 27074 / LMG 4051 / NBRC 15346 / NCIMB 9279 / VKM B-1422 / R1) (see 2 papers)
45% identity, 95% coverage: 13:362/370 of query aligns to 19:367/375 of Q9RYA6

query
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Q9RYA6

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V48 (≠ N43) mutation to C: Loss of activity; when associated with C-120.
M56 (≠ A51) mutation to C: Loss of activity; when associated with C-65.
P60 (= P55) mutation to C: Loss of activity; when associated with C-100.
E65 (= E60) mutation to C: Loss of activity; when associated with C-56.
A68 (≠ G63) mutation to C: No change in activity; when associated with C-72.
D72 (≠ H67) mutation to C: No change in activity; when associated with C-68.
Y100 (≠ I95) mutation to C: Loss of activity; when associated with C-60.
R120 (≠ K115) mutation to C: Loss of activity; when associated with C-48.
G127 (≠ A122) mutation to C: Retains 93% of wild-type activity; when associated with C-313.
S142 (= S137) binding N-acetyl-L-glutamine
E149 (≠ V144) mutation to C: Retains 88% of wild-type activity; when associated with C-182.
G163 (= G158) mutation to C: Loss of activity; when associated with C-343.
KLK 168:170 (≠ KIK 163:165) binding N-acetyl-L-glutamine
A182 (≠ N177) mutation to C: Retains 88% of wild-type activity; when associated with C-149.
D195 (= D190) binding Mg(2+)
Y218 (≠ M213) mutation to C: Retains 35% of wild-type activity.
E220 (= E215) binding Mg(2+)
D245 (= D240) binding Mg(2+)
V265 (≠ I260) mutation to C: Retains 39% of wild-type activity.
K269 (= K264) binding N-acetyl-L-glutamine
L299 (= L294) binding N-acetyl-L-glutamine
T313 (= T308) mutation to C: Retains 93% of wild-type activity; when associated with C-127.
D343 (≠ N338) mutation to C: Loss of activity; when associated with C-163.

2gghC The mutant a68c-d72c-nlq of deinococcus radiodurans nacylamino acid racemase (see paper)
45% identity, 95% coverage: 13:362/370 of query aligns to 14:362/370 of 2gghC

query
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2gghC

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A

L

I

Q

L

E

G

N

Q

A

T

I

F

S

A

H

N

P

P

D

E

D

A

V

V

S

S

A

D

F

A

F

L

Q

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P

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I
x
Y

K

R

R

G

N

N

N

R

M

M

A

A

K

R

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A

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M

L

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E

E

G

M

A

A

V

A

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W

D

D

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W

A

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A

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E

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S

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A

A

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L

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I

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I

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G

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W

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D

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I

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x
R

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D

A

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A

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Y

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T

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A

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A

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R

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K

A

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E

A

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E

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S

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C

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G

M
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M

L
|
L

E

E

A

S

G

G

V

I

G

G

R

R

A

A

H

H

N

N

I

I

A

H

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T

T

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L

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G

N

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F

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R

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L

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D

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T

A

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A

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Y

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W

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E

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R

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D

I

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A

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active site: F21 (= F20), Y95 (≠ I95), S137 (= S137), K163 (= K163), K165 (= K165), R186 (≠ P186), T188 (≠ M188), D190 (= D190), N192 (= N192), E215 (= E215), D240 (= D240), E241 (= E241), S242 (= S242), K264 (= K264), C290 (= C290), G291 (= G291), G292 (= G292), M293 (= M293), G316 (= G316), D317 (= D317), T318 (= T318)
binding magnesium ion: D190 (= D190), E215 (= E215)
binding n~2~-acetyl-l-glutamine: F21 (= F20), G292 (= G292), M293 (= M293), L294 (= L294)

Q44244 N-succinylamino acid racemase; NSAR; N-acylamino acid racemase; AAR; NAAAR; o-succinylbenzoate synthase; OSB synthase; OSBS; EC 5.1.1.-; EC 5.1.1.-; EC 4.2.1.113 from Amycolatopsis sp. (see 5 papers)
43% identity, 97% coverage: 4:362/370 of query aligns to 3:360/368 of Q44244

query
sites
Q44244

I

L

K

S

Q

G

I

V

K

E

L

L

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R

K

R

L

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x
R

T
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T

S
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S

F
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F

G
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G

T

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E

F

L

I

L

A

L

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A

A

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P

E

A

G

G

N

E

R

-

G

G

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W

G

G

E

E

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C

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A

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F

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S

A

Y

G

P

P

W

L

Y

Y

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S

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N

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D

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G

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Y

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A

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A

N

A

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I

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A

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A

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A

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M

A

A

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A

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E

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M

A

A

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D

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Y

E

A

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A

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N

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A

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A

A

E

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L

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G

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C

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S

I

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G

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x
D

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I

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P

A

Q

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L

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D

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Y

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x
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P

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G

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W

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D

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A

A

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A

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D

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A

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A

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H

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G

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W

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C

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M

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x
I

E

E

A

T

G

G

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L

G

G

R

R

A

A

H

A

N

N

I

V

A

A

L

L

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A

T

S

L

L

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P

G

N

F

F

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T

L

L

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P

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G

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D

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x
F

W

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D

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I

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P

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F

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-

V

L

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S

N

G

G

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P18 (= P19) mutation to A: Small increase in OSBS catalytic efficiency and 2-fold decrease in NSAR catalytic efficiency.
F19 (= F20) mutation to A: 200-fold decrease in OSBS catalytic efficiency and 120-fold decrease in NSAR catalytic efficiency. 2100-fold decrease in OSBS catalytic efficiency and 180-fold decrease in NSAR catalytic efficiency; when associated with A-23.
R20 (≠ T21) mutation to E: 32-fold decrease in OSBS catalytic efficiency and 8-fold decrease in NSAR catalytic efficiency.
T21 (= T22) mutation to A: 4-fold decrease in OSBS and NSAR catalytic efficiencies.
S22 (= S23) mutation to R: 3-fold increase in OSBS catalytic efficiency and 4-fold decrease in NSAR catalytic efficiency.
F23 (= F24) mutation to A: 2100-fold decrease in OSBS catalytic efficiency and 180-fold decrease in NSAR catalytic efficiency; when associated with A-19.
G24 (= G25) mutation to A: Small decrease in OSBS catalytic efficiency and 4-fold decrease in NSAR catalytic efficiency.
S135 (= S137) binding 2-succinylbenzoate
D140 (≠ P142) mutation to R: 3-fold decrease in OSBS and NSAR catalytic efficiencies.
KLK 161:163 (≠ KIK 163:165) binding 2-succinylbenzoate
K163 (= K165) mutation K->A,R,S: Significantly impairs both NAAAR and OSBS activities.
D189 (= D190) binding Mg(2+)
N191 (= N192) binding 2-succinylbenzoate
E214 (= E215) binding Mg(2+)
D239 (= D240) binding Mg(2+)
K263 (= K264) mutation K->R,S: Significantly impairs both NAAAR and OSBS activities.
G291 (= G292) mutation to D: Shows up to 6-fold higher activity than the wild-type on a range of N-acetylated amino acids; when associated with Y-323.
I293 (≠ L294) binding 2-succinylbenzoate
F323 (≠ Y324) mutation to Y: Shows up to 6-fold higher activity than the wild-type on a range of N-acetylated amino acids; when associated with D-291.

1sjdA X-ray structure of o-succinylbenzoate synthase complexed with n- succinyl phenylglycine (see paper)
43% identity, 97% coverage: 4:362/370 of query aligns to 3:360/367 of 1sjdA

query
sites
1sjdA

I

L

K

S

Q

G

I

V

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E

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L

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R

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R

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Q

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M

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P

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L

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A

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F
|
F

T

R

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T

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S

F
|
F

G

G

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T

M

Q

Q

S

D

V

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R

E

E

F

L

I

L

A

L

E

R

A

A

I

V

D

T

G

P

E

A

G

G

N

E

R

-

G

G

F

W

G

G

E

E

S

C

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V

A

T

F
x
M

S

A

Y

G

P

P

W

L

Y

Y

S

S

E

S

E

E

T

Y

V

N

G
x
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T
x
G

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|
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D

H

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Y

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L

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A

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A

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A

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M

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A

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A

A

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S

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D

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Y

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I

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G

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D

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A

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R

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D

I

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D

A

A

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A

A

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Y

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T

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L

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G

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A

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E

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L

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E

D

D

I

V

I

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H

A

A

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E

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C

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S
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S

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D

A

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K

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E

A

A

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R

I

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H

D

D

Y

V

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C

R

A

N

A

N

H

G

G

I

I

D

P

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W

W

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C

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G

M
|
M

L
x
I

E

E

A

T

G

G

V

L

G

G

R

R

A

A

H

A

N

N

I

V

A

A

L

L

T

A

T

S

L

L

P

P

G

N

F

F

T

T

L

L

P

P

G
|
G

D
|
D

T
|
T

A

S

G

A

S

S

L

D

L

R

Y
x
F

W

Y

E

K

K

T

D

D

I

I

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K

E

P

P

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F

V

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N

-

V

L

E

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N

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G

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H

I

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N

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T

active site: F19 (= F20), S135 (= S137), K161 (= K163), K163 (= K165), D189 (= D190), N191 (= N192), E214 (= E215), D239 (= D240), E240 (= E241), S241 (= S242), K263 (= K264), G290 (= G291), G291 (= G292), M292 (= M293), G315 (= G316), D316 (= D317), T317 (= T318)
binding n-succinyl phenylglycine: F23 (= F24), M50 (≠ F52), D61 (≠ G63), G62 (≠ T64), H65 (= H67), S135 (= S137), K163 (= K165), N191 (= N192), K263 (= K264), G291 (= G292), M292 (= M293), I293 (≠ L294), F323 (≠ Y324)

1sjcA X-ray structure of o-succinylbenzoate synthase complexed with n- succinyl methionine (see paper)
43% identity, 97% coverage: 4:362/370 of query aligns to 3:360/367 of 1sjcA

query
sites
1sjcA

I

L

K

S

Q

G

I

V

K

E

L

L

R

R

K

R

L

V

N

Q

M

M

H

P

L

L

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H

A

P

P

F
|
F

T

R

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S

S

F
|
F

G

G

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T

M

Q

Q

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E

E

F

L

I

L

A

L

E

R

A

A

I

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P

E

A

G

G

N

E

R

-

G

G

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W

G

G

E

E

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x
M

S

A

Y

G

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P

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L

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Y

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S

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E

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N

G

D

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G

N

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A

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A

A

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I

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P

A

D

A

D

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A

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I

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K

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M

M

A

A

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G

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A

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L

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E

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M

A

A

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D

L

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E

A

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A

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N

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A

A

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A

A

E

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L

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G

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R

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C

G

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|
S

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M

P

D

S

T

V

I

E

P

A

Q

L

L

D

D

K

V

V

V

D

G

Y

G

F

Y

V

L

N

D

E

E

G

G

Y

Y

K

V

R

R

I

I

K
|
K

I

L

K
|
K

I

I

K

E

P

P

G

G

W

W

D

D

V

V

D

E

V

P

L

V

Q

R

N

A

V

V

R

R

E

E

R

R

F

F

P

G

E

D

-

D

I

V

P

L

I

L

M

Q

A

V

D
|
D

A

A

N
|
N

S

T

A

A

Y

Y

T

T

L

L

N

G

D

D

M

A

E

P

H

Q

L

L

K

A

Q

R

L

L

D

D

D

P

L

F

N

G

L

L

M

L

I

I

E
|
E

Q

Q

P

P

L

L

A

E

H

E

D

E

D

D

I

V

I

L

D

G

H

H

A

A

T

E

L

L

Q

A

A

R

N

R

M

I

A

Q

T

T

P

P

V

I

C

C

L

L

D
|
D

E
|
E

S
|
S

I

I

H

V

S

S

L

A

E

R

D

A

T

A

R

A

K

D

A

A

V

I

E

K

L

L

G

G

S

A

C

V

K

Q

I

I

I

V

N

N

I

I

K
|
K

I

P

G

G

R

R

V

V

G

G

L

Y

S

L

E

E

A

A

K

R

R

R

I

V

H

H

D

D

Y

V

C

C

R

A

N

A

N

H

G

G

I

I

D

P

V

V

W

W

C

C

G
|
G

M
|
M

L
x
I

E

E

A

T

G

G

V

L

G

G

R

R

A

A

H

A

N

N

I

V

A

A

L

L

T

A

T

S

L

L

P

P

G

N

F

F

T

T

L

L

P

P

G
|
G

D
|
D

T
|
T

A

S

G

A

S

S

L

D

L

R

Y
x
F

W

Y

E

K

K

T

D

D

I

I

I

T

K

E

P

P

V

F

V

V

N

-

V

L

E

S

N

G

G

G

V

H

I

L

Q

P

V

V

P

P

N

T

K

G

P

P

G

G

I

L

G

G

Y

V

E

A

I

P

D

I

E

P

E

E

A

L

L

L

E

D

E

E

F

V

T

T

active site: F19 (= F20), S135 (= S137), K161 (= K163), K163 (= K165), D189 (= D190), N191 (= N192), E214 (= E215), D239 (= D240), E240 (= E241), S241 (= S242), K263 (= K264), G290 (= G291), G291 (= G292), M292 (= M293), G315 (= G316), D316 (= D317), T317 (= T318)
binding magnesium ion: D189 (= D190), E214 (= E215), D239 (= D240)
binding n-succinyl methionine: F19 (= F20), F23 (= F24), M50 (≠ F52), S135 (= S137), K161 (= K163), K163 (= K165), D189 (= D190), N191 (= N192), D239 (= D240), K263 (= K264), G291 (= G292), M292 (= M293), I293 (≠ L294), F323 (≠ Y324)

1sjbA X-ray structure of o-succinylbenzoate synthase complexed with o- succinylbenzoic acid (see paper)
43% identity, 97% coverage: 4:362/370 of query aligns to 3:360/367 of 1sjbA

query
sites
1sjbA

I

L

K

S

Q

G

I

V

K

E

L

L

R

R

K

R

L

V

N

Q

M

M

H

P

L

L

K

V

H

A

P

P

F
|
F

T

R

T

T

S

S

F
|
F

G

G

T

T

M

Q

Q

S

D

V

K

R

E

E

F

L

I

L

A

L

E

R

A

A

I

V

D

T

G

P

E

A

G

G

N

E

R

-

G

G

F

W

G

G

E

E

S

C

V

V

A

T

F

M

S

A

Y

G

P

P

W

L

Y
|
Y

S

S

E

S

E

E

T

Y

V

N

G

D

T

G

N

A

W

E

H

H

V

V

M

L

T

R

D

H

F

Y

L

L

I

I

P

P

I

A

L

L

Q

L

E

A

N

A

A

E

I

D

S

I

H

T

P

A

D

A

D

K

V

V

S

T

A

P

F

L

Q

A

P

K

I

F

K

K

R

G

N

H

N

R

M

M

A

A

K

K

S

G

T

A

L

L

E

E

G

M

A

A

V

V

W

L

D

D

L

A

Y

E

A

L

K

R

Q

A

N

H

N

E

K

R

T

S

L

F

A

A

S

A

A

E

L

L

G

G

G

S

E

V

K

R

E

D

E

S

I

V

G

P

V

C

G

G

I

V

S
|
S

I

V

G

G

I

I

Q

M

P

D

S

T

V

I

E

P

A

Q

L

L

D

D

K

V

V

V

D

G

Y

G

F

Y

V

L

N

D

E

E

G

G

Y

Y

K

V

R

R

I

I

K
|
K

I

L

K
|
K

I

I

K

E

P

P

G

G

W

W

D

D

V

V

D

E

V

P

L

V

Q

R

N

A

V

V

R

R

E

E

R

R

F

F

P

G

E

D

-

D

I

V

P

L

I

L

M

Q

A

V

D
|
D

A

A

N
|
N

S

T

A

A

Y

Y

T

T

L

L

N

G

D

D

M

A

E

P

H

Q

L

L

K

A

Q

R

L

L

D

D

D

P

L

F

N

G

L

L

M

L

I

I

E
|
E

Q

Q

P

P

L

L

A

E

H

E

D

E

D

D

I

V

I

L

D

G

H

H

A

A

T

E

L

L

Q

A

A

R

N

R

M

I

A

Q

T

T

P

P

V

I

C

C

L

L

D
|
D

E
|
E

S
|
S

I

I

H

V

S

S

L

A

E

R

D

A

T

A

R

A

K

D

A

A

V

I

E

K

L

L

G

G

S

A

C

V

K

Q

I

I

I

V

N

N

I

I

K
|
K

I

P

G

G

R

R

V

V

G

G

L

Y

S

L

E

E

A

A

K

R

R

R

I

V

H

H

D

D

Y

V

C

C

R

A

N

A

N

H

G

G

I

I

D

P

V

V

W

W

C

C

G
|
G

M
|
M

L
x
I

E

E

A

T

G

G

V

L

G

G

R

R

A

A

H

A

N

N

I

V

A

A

L

L

T

A

T

S

L

L

P

P

G

N

F

F

T

T

L

L

P

P

G
|
G

D
|
D

T
|
T

A

S

G

A

S

S

L

D

L

R

Y
x
F

W

Y

E

K

K

T

D

D

I

I

I

T

K

E

P

P

V

F

V

V

N

-

V

L

E

S

N

G

G

G

V

H

I

L

Q

P

V

V

P

P

N

T

K

G

P

P

G

G

I

L

G

G

Y

V

E

A

I

P

D

I

E

P

E

E

A

L

L

L

E

D

E

E

F

V

T

T

active site: F19 (= F20), S135 (= S137), K161 (= K163), K163 (= K165), D189 (= D190), N191 (= N192), E214 (= E215), D239 (= D240), E240 (= E241), S241 (= S242), K263 (= K264), G290 (= G291), G291 (= G292), M292 (= M293), G315 (= G316), D316 (= D317), T317 (= T318)
binding magnesium ion: D189 (= D190), E214 (= E215), D239 (= D240)
binding 2-succinylbenzoate: F23 (= F24), Y55 (= Y57), K161 (= K163), K163 (= K165), D189 (= D190), N191 (= N192), D239 (= D240), K263 (= K264), G291 (= G292), M292 (= M293), I293 (≠ L294), F323 (≠ Y324)

5fjtA N-acyl amino acid racemase from amycolatopsis sp. Ts-1-60: g291d f323 mutant in complex with n-acetyl phenylalanine
43% identity, 97% coverage: 4:362/370 of query aligns to 3:360/367 of 5fjtA

query
sites
5fjtA

I

L

K

S

Q

G

I

V

K

E

L

L

R

R

K

R

L

V

N

Q

M

M

H

P

L

L

K

V

H

A

P

P

F
|
F

T

R

T

T

S

S

F

F

G

G

T

T

M

Q

Q

S

D

V

K

R

E

E

F

L

I

L

A

L

E

R

A

A

I

V

D

T

G

P

E

A

G

G

N

E

R

-

G

G

F

W

G

G

E

E

S

C

V

V

A

T

F
x
M

S

A

Y

G

P

P

W

L

Y

Y

S

S

E

S

E

E

T

Y

V

N

G

D

T

G

N

A

W

E

H

H

V

V

M

L

T

R

D

H

F

Y

L

L

I

I

P

P

I

A

L

L

Q

L

E

A

N

A

A

E

I

D

S

I

H

T

P

A

D

A

D

K

V

V

S

T

A

P

F

L

Q

A

P

K

I

F

K

K

R

G

N

H

N

R

M

M

A

A

K

K

S

G

T

A

L

L

E

E

G

M

A

A

V

V

W

L

D

D

L

A

Y

E

A

L

K

R

Q

A

N

H

N

E

K

R

T

S

L

F

A

A

S

A

A

E

L

L

G

G

G

S

E

V

K

R

E

D

E

S

I

V

G

P

V

C

G

G

I

V

S
|
S

I

V

G

G

I

I

Q

M

P

D

S

T

V

I

E

P

A

Q

L

L

D

D

K

V

V

V

D

G

Y

G

F

Y

V

L

N

D

E

E

G

G

Y

Y

K

V

R

R

I

I

K
|
K

I

L

K
|
K

I

I

K

E

P

P

G

G

W

W

D

D

V

V

D

E

V

P

L

V

Q

R

N

A

V

V

R

R

E

E

R

R

F

F

-

G

P

D

E

D

I

V

P

L

I

L

M

Q

A

V

D
|
D

A

A

N
|
N

S

T

A

A

Y

Y

T

T

L

L

N

G

D

D

M

A

E

P

H

Q

L

L

K

A

Q

R

L

L

D

D

D

P

L

F

N

G

L

L

M

L

I

I

E
|
E

Q

Q

P

P

L

L

A

E

H

E

D

E

D

D

I

V

I

L

D

G

H

H

A

A

T

E

L

L

Q

A

A

R

N

R

M

I

A

Q

T

T

P

P

V

I

C

C

L

L

D
|
D

E
|
E

S
|
S

I

I

H

V

S

S

L

A

E

R

D

A

T

A

R

A

K

D

A

A

V

I

E

K

L

L

G

G

S

A

C

V

K

Q

I

I

I

V

N

N

I

I

K
|
K

I

P

G

G

R

R

V

V

G

G

L

Y

S

L

E

E

A

A

K

R

R

R

I

V

H

H

D

D

Y

V

C

C

R

A

N

A

N

H

G

G

I

I

D

P

V

V

W

W

C

C

G
|
G

G
x
D

M
|
M

L
x
I

E

E

A

T

G

G

V

L

G

G

R

R

A

A

H

A

N

N

I

V

A

A

L

L

T

A

T

S

L

L

P

P

G

N

F

F

T

T

L

L

P

P

G
|
G

D
|
D

T
|
T

A

S

G

A

S

S

L

D

L

R

Y

Y

W

Y

E

K

K

T

D

D

I

I

I

T

K

E

P

P

V

F

V

V

N

-

V

L

E

S

N

G

G

G

V

H

I

L

Q

P

V

V

P

P

N

T

K

G

P

P

G

G

I

L

G

G

Y

V

E

A

I

P

D

I

E

P

E

E

A

L

L

L

E

D

E

E

F

V

T

T

active site: F19 (= F20), S135 (= S137), K161 (= K163), K163 (= K165), D189 (= D190), N191 (= N192), E214 (= E215), D239 (= D240), E240 (= E241), S241 (= S242), K263 (= K264), G290 (= G291), D291 (≠ G292), M292 (= M293), G315 (= G316), D316 (= D317), T317 (= T318)
binding N-acetyl-L-phenylalanine: F19 (= F20), M50 (≠ F52), S135 (= S137), K163 (= K165), N191 (= N192), K263 (= K264), D291 (≠ G292), M292 (= M293), I293 (≠ L294)
binding magnesium ion: D189 (= D190), E214 (= E215), D239 (= D240)

5fjoA N-acyl amino acid racemase from amycolatopsis sp. Ts-1-60: g291d- f323y mutant in complex with n-acetyl naphthylalanine
43% identity, 97% coverage: 4:362/370 of query aligns to 3:360/367 of 5fjoA

query
sites
5fjoA

I

L

K

S

Q

G

I

V

K

E

L

L

R

R

K

R

L

V

N

Q

M

M

H

P

L

L

K

V

H

A

P

P

F
|
F

T

R

T

T

S

S

F

F

G

G

T

T

M
x
Q

Q

S

D

V

K
x
R

E

E

F

L

I

L

A

L

E

R

A

A

I

V

D

T

G

P

E

A

G

G

N

E

R

-

G

G

F

W

G

G

E

E

S

C

V

V

A

T

F
x
M

S

A

Y

G

P

P

W

L

Y

Y

S

S

E

S

E

E

T

Y

V

N

G

D

T

G

N

A

W

E

H

H

V

V

M

L

T

R

D

H

F

Y

L

L

I

I

P

P

I

A

L

L

Q

L

E

A

N

A

A

E

I

D

S

I

H

T

P

A

D

A

D

K

V

V

S

T

A

P

F

L

Q

A

P

K

I

F

K

K

R

G

N

H

N

R

M

M

A

A

K

K

S

G

T

A

L

L

E

E

G

M

A

A

V

V

W

L

D

D

L

A

Y

E

A

L

K

R

Q

A

N

H

N

E

K

R

T

S

L

F

A

A

S

A

A

E

L

L

G

G

G

S

E

V

K

R

E

D

E

S

I

V

G

P

V

C

G

G

I

V

S
|
S

I

V

G

G

I

I

Q

M

P

D

S

T

V

I

E

P

A

Q

L

L

D

D

K

V

V

V

D

G

Y

G

F

Y

V

L

N

D

E

E

G

G

Y

Y

K

V

R

R

I

I

K
|
K

I

L

K
|
K

I

I

K

E

P

P

G

G

W

W

D

D

V

V

D

E

V

P

L

V

Q

R

N

A

V

V

R

R

E

E

R

R

F

F

-

G

P

D

E

D

I

V

P

L

I

L

M

Q

A

V

D
|
D

A

A

N
|
N

S

T

A

A

Y

Y

T

T

L

L

N

G

D

D

M

A

E

P

H

Q

L

L

K

A

Q

R

L

L

D

D

D

P

L

F

N

G

L

L

M

L

I

I

E
|
E

Q

Q

P

P

L

L

A

E

H

E

D

E

D

D

I

V

I

L

D

G

H

H

A

A

T

E

L

L

Q

A

A

R

N

R

M

I

A

Q

T

T

P

P

V

I

C

C

L

L

D
|
D

E
|
E

S
|
S

I

I

H

V

S

S

L

A

E

R

D

A

T

A

R

A

K

D

A

A

V

I

E

K

L

L

G

G

S

A

C

V

K

Q

I

I

I

V

N

N

I

I

K
|
K

I

P

G

G

R

R

V

V

G

G

L

Y

S

L

E

E

A

A

K

R

R

R

I

V

H

H

D

D

Y

V

C

C

R

A

N

A

N

H

G

G

I

I

D

P

V

V

W

W

C

C

G
|
G

G
x
D

M
|
M

L
x
I

E

E

A

T

G

G

V

L

G

G

R

R

A

A

H

A

N

N

I

V

A

A

L

L

T

A

T

S

L

L

P

P

G

N

F

F

T

T

L

L

P

P

G
|
G

D
|
D

T
|
T

A

S

G

A

S

S

L

D

L

R

Y

Y

W

Y

E

K

K

T

D

D

I

I

I

T

K

E

P

P

V

F

V

V

N

-

V

L

E

S

N

G

G

G

V

H

I

L

Q

P

V

V

P

P

N

T

K

G

P

P

G

G

I

L

G

G

Y

V

E

A

I

P

D

I

E

P

E

E

A

L

L

L

E

D

E

E

F

V

T

T

active site: F19 (= F20), S135 (= S137), K161 (= K163), K163 (= K165), D189 (= D190), N191 (= N192), E214 (= E215), D239 (= D240), E240 (= E241), S241 (= S242), K263 (= K264), G290 (= G291), D291 (≠ G292), M292 (= M293), G315 (= G316), D316 (= D317), T317 (= T318)
binding magnesium ion: D189 (= D190), E214 (= E215), D239 (= D240)
binding N-acetyl naphthylalanine: F19 (= F20), Q26 (≠ M27), R29 (≠ K30), M50 (≠ F52), S135 (= S137), K161 (= K163), K163 (= K165), N191 (= N192), K263 (= K264), D291 (≠ G292), I293 (≠ L294)

4a6gA N-acyl amino acid racemase from amycalotopsis sp. Ts-1-60: g291d- f323y mutant in complex with n-acetyl methionine (see paper)
43% identity, 97% coverage: 4:362/370 of query aligns to 3:360/368 of 4a6gA

query
sites
4a6gA

I

L

K

S

Q

G

I

V

K

E

L

L

R

R

K

R

L

V

N

Q

M

M

H

P

L

L

K

V

H

A

P

P

F
|
F

T

R

T

T

S

S

F
|
F

G

G

T

T

M

Q

Q

S

D

V

K

R

E

E

F

L

I

L

A

L

E

R

A

A

I

V

D

T

G

P

E

A

G

G

N

E

R

-

G

G

F

W

G

G

E

E

S

C

V

V

A

T

F

M

S

A

Y

G

P

P

W

L

Y

Y

S

S

E

S

E

E

T

Y

V

N

G

D

T

G

N

A

W

E

H

H

V

V

M

L

T

R

D

H

F

Y

L

L

I

I

P

P

I

A

L

L

Q

L

E

A

N

A

A

E

I

D

S

I

H

T

P

A

D

A

D

K

V

V

S

T

A

P

F

L

Q

A

P

K

I

F

K

K

R

G

N

H

N

R

M

M

A

A

K

K

S

G

T

A

L

L

E

E

G

M

A

A

V

V

W

L

D

D

L

A

Y

E

A

L

K

R

Q

A

N

H

N

E

K

R

T

S

L

F

A

A

S

A

A

E

L

L

G

G

G

S

E

V

K

R

E

D

E

S

I

V

G

P

V

C

G

G

I

V

S
|
S

I

V

G

G

I

I

Q

M

P

D

S

T

V

I

E

P

A

Q

L

L

D

D

K

V

V

V

D

G

Y

G

F

Y

V

L

N

D

E

E

G

G

Y

Y

K

V

R

R

I

I

K
|
K

I

L

K
|
K

I

I

K

E

P

P

G

G

W

W

D

D

V

V

D

E

V

P

L

V

Q

R

N

A

V

V

R

R

E

E

R

R

F

F

-

G

P

D

E

D

I

V

P

L

I

L

M

Q

A

V

D
|
D

A

A

N
|
N

S

T

A

A

Y

Y

T

T

L

L

N

G

D

D

M

A

E

P

H

Q

L

L

K

A

Q

R

L

L

D

D

D

P

L

F

N

G

L

L

M

L

I

I

E
|
E

Q

Q

P

P

L

L

A

E

H

E

D

E

D

D

I

V

I

L

D

G

H

H

A

A

T

E

L

L

Q

A

A

R

N

R

M

I

A

Q

T

T

P

P

V

I

C

C

L

L

D
|
D

E
|
E

S
|
S

I

I

H

V

S

S

L

A

E

R

D

A

T

A

R

A

K

D

A

A

V

I

E

K

L

L

G

G

S

A

C

V

K

Q

I

I

I

V

N

N

I

I

K
|
K

I

P

G

G

R

R

V

V

G

G

L

Y

S

L

E

E

A

A

K

R

R

R

I

V

H

H

D

D

Y

V

C

C

R

A

N

A

N

H

G

G

I

I

D

P

V

V

W

W

C

C

G
|
G

G
x
D

M
|
M

L

I

E

E

A

T

G

G

V

L

G

G

R

R

A

A

H

A

N

N

I

V

A

A

L

L

T

A

T

S

L

L

P

P

G

N

F

F

T

T

L

L

P

P

G
|
G

D
|
D

T
|
T

A

S

G

A

S

S

L

D

L

R

Y

Y

W

Y

E

K

K

T

D

D

I

I

I

T

K

E

P

P

V

F

V

V

N

-

V

L

E

S

N

G

G

G

V

H

I

L

Q

P

V

V

P

P

N

T

K

G

P

P

G

G

I

L

G

G

Y

V

E

A

I

P

D

I

E

P

E

E

A

L

L

L

E

D

E

E

F

V

T

T

active site: F19 (= F20), S135 (= S137), K161 (= K163), K163 (= K165), D189 (= D190), N191 (= N192), E214 (= E215), D239 (= D240), E240 (= E241), S241 (= S242), K263 (= K264), G290 (= G291), D291 (≠ G292), M292 (= M293), G315 (= G316), D316 (= D317), T317 (= T318)
binding n-acetylmethionine: F19 (= F20), F23 (= F24), S135 (= S137), K163 (= K165), K263 (= K264), D291 (≠ G292)
binding magnesium ion: D189 (= D190), E214 (= E215), D239 (= D240)

7s8wA Amycolatopsis sp. T-1-60 n-succinylamino acid racemase/o- succinylbenzoate synthase r266q mutant in complex with n- succinylphenylglycine (see paper)
43% identity, 97% coverage: 4:362/370 of query aligns to 3:360/367 of 7s8wA

query
sites
7s8wA

I

L

K

S

Q

G

I

V

K

E

L

L

R

R

K

R

L

V

N

Q

M

M

H

P

L

L

K

V

H

A

P

P

F
|
F

T

R

T

T

S

S

F
|
F

G

G

T

T

M

Q

Q

S

D

V

K

R

E

E

F

L

I

L

A

L

E

R

A

A

I

V

D

T

G

P

E

A

G

G

N

E

R

-

G

G

F

W

G

G

E

E

S

C

V

V

A

T

F

M

S

A

Y

G

P

P

W

L

Y
|
Y

S

S

E

S

E

E

T

Y

V

N

G

D

T

G

N

A

W

E

H

H

V

V

M

L

T

R

D

H

F

Y

L

L

I

I

P

P

I

A

L

L

Q

L

E

A

N

A

A

E

I

D

S

I

H

T

P

A

D

A

D

K

V

V

S

T

A

P

F

L

Q

A

P

K

I

F

K

K

R

G

N

H

N

R

M

M

A

A

K

K

S

G

T

A

L

L

E

E

G

M

A

A

V

V

W

L

D

D

L

A

Y

E

A

L

K

R

Q

A

N

H

N

E

K

R

T

S

L

F

A

A

S

A

A

E

L

L

G

G

G

S

E

V

K

R

E

D

E

S

I

V

G

P

V

C

G

G

I

V

S
|
S

I

V

G

G

I

I

Q

M

P

D

S

T

V

I

E

P

A

Q

L

L

D

D

K

V

V

V

D

G

Y

G

F

Y

V

L

N

D

E

E

G

G

Y

Y

K

V

R

R

I

I

K
|
K

I

L

K
|
K

I

I

K

E

P

P

G

G

W

W

D

D

V

V

D

E

V

P

L

V

Q

R

N

A

V

V

R

R

E

E

R

R

F

F

-

G

P

D

E

D

I

V

P

L

I

L

M

Q

A

V

D
|
D

A

A

N
|
N

S

T

A

A

Y

Y

T

T

L

L

N

G

D

D

M

A

E

P

H

Q

L

L

K

A

Q

R

L

L

D

D

D

P

L

F

N

G

L

L

M

L

I

I

E
|
E

Q

Q

P

P

L

L

A

E

H

E

D

E

D

D

I

V

I

L

D

G

H

H

A

A

T

E

L

L

Q

A

A

R

N

R

M

I

A

Q

T

T

P

P

V

I

C

C

L

L

D
|
D