SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_010531995.1 NCBI__GCF_000224785.1:WP_010531995.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9RW55 Proline dehydrogenase; PRODH; DrPRODH; Proline oxidase; EC 1.5.5.2 from Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / CCUG 27074 / LMG 4051 / NBRC 15346 / NCIMB 9279 / VKM B-1422 / R1) (see paper)
41% identity, 81% coverage: 23:298/341 of query aligns to 26:305/310 of Q9RW55

query
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Q9RW55

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G63 (= G63) mutation to A: 140-fold decrease in catalytic efficiency for proline.
E64 (= E64) mutation to A: 27-fold decrease in catalytic efficiency for proline.
K98 (= K98) binding substrate
M136 (= M133) binding FAD
Q166 (= Q161) binding FAD
RMVKGA 187:192 (≠ RIVKGA 180:185) binding FAD
TH 229:230 (= TH 222:223) binding FAD
RR 291:292 (= RR 284:285) binding substrate
RIAE 292:295 (≠ RLAE 285:288) binding FAD

4h6qA Structure of oxidized deinococcus radiodurans proline dehydrogenase complexed with l-tetrahydrofuroic acid (see paper)
42% identity, 79% coverage: 23:290/341 of query aligns to 10:281/281 of 4h6qA

query
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4h6qA

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binding flavin-adenine dinucleotide: D119 (= D132), M120 (= M133), Q150 (= Q161), R171 (= R180), V173 (= V182), K174 (= K183), G175 (= G184), A176 (= A185), A212 (≠ G221), T213 (= T222), H214 (= H223), M240 (= M249), L241 (= L250), I244 (≠ F253), R276 (= R285), E279 (= E288), P281 (= P290)
binding tetrahydrofuran-2-carboxylic acid: K82 (= K98), Y262 (= Y271), R275 (= R284), R276 (= R285)

4h6rA Structure of reduced deinococcus radiodurans proline dehydrogenase (see paper)
42% identity, 79% coverage: 23:290/341 of query aligns to 1:272/272 of 4h6rA

query
sites
4h6rA

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binding dihydroflavine-adenine dinucleotide: D110 (= D132), M111 (= M133), V139 (= V159), Q141 (= Q161), R162 (= R180), V164 (= V182), K165 (= K183), G166 (= G184), A167 (= A185), A203 (≠ G221), T204 (= T222), H205 (= H223), Q230 (= Q248), L232 (= L250), I235 (≠ F253), Y253 (= Y271), R267 (= R285), E270 (= E288), P272 (= P290)

Q72IB8 Proline dehydrogenase; PRODH; Proline oxidase; TtPRODH; EC 1.5.5.2 from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see 2 papers)
39% identity, 84% coverage: 6:292/341 of query aligns to 8:296/307 of Q72IB8

query
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Q72IB8

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M134 (= M133) binding FAD
Q163 (= Q161) binding FAD
KGA 187:189 (= KGA 183:185) binding FAD
K201 (= K197) binding FAD
TH 226:227 (= TH 222:223) binding FAD

2g37B Structure of thermus thermophilus l-proline dehydrogenase (see paper)
39% identity, 84% coverage: 6:290/341 of query aligns to 14:300/300 of 2g37B

query
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2g37B

R

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E

V

A

M

L

E

K

A

A

V

A

K

E

E

A

L

L

N

E

H

R

Q

E

G

G

I

V

S

H

C

A

T

I

L

L

D

D

N

L

L

L

G

G

E

E

F

M

V

V

S

R

D

T

K

E

T

E

E

E

A

A

G

R

E

A

A

F

K

Q

D

R

K

G

I

L

R

E

L

L

L

V

N

W

T

A

I

L

H

A

E

G

E

K

N

P

V

W

D

P

C

K

H

Y

L

I

S

S

V

L

K

K

L

L

T

T

Q

Q

L

L

G

G

L

L

D

D

I

L

D

S

Q

E

A

D

Y

L

C

A

T

L

E

A

N

L

M

L

R

R

E

E

I

V

L

L

D

R

I

E

A

A

R

E

Q

P

Y

R

N

G

I

V

F

F

V

V

N

R

I

L

D
|
D

M
|
M

E

E

D

D

Y

S

S

P

H

R

Y

V

D

E

Q

A

T

T

L

L

E

R

V

L

L

Y

Q

R

A

A

L

L

R

R

K

E

D

E

-

G

Y

F

D

S

N

Q

V

V

G

G

T

I

V
|
V

I

L

Q

Q

S

S

Y

Y

L

L

Y

Y

R

R

A

T

E

E

E

K

D

D

M

L

E

L

N

D

L

L

K

L

N

P

V

Y

R

R

-

P

-

N

L

L

R
|
R

I

L

V
|
V

K
|
K

G
|
G

A
|
A

Y

Y

K

R

E

E

S

P

E

K

E

E

V

V

A

A

Y

F

P

P

E

D

K

K

Q

R

D

L

I

I

D

D

R

A

N

E

F

Y

L

L

E

H

L

L

A

G

K

K

Q

L

R

A

L

L

A

K

G

E

D

G

T

L

F

Y

T

V

S

A

I

F

G
x
A

T
|
T

H
|
H

D
|
D

H

P

H

R

I

I

N

A

E

E

L

L

K

K

A

R

F

Y

A

T

D

E

E

A

H

M

N

G

I

I

D

P

H

R

D

S

K

R

F

F

E

E

F

F

Q

Q

M

F

L
|
L

Y

Y

G

G

F
x
V

R

R

N

P

D

E

M

E

Q

Q

N

R

K

R

L

L

A

A

N

R

E

E

G

G

Y

Y

N

T

F

V

C

R

T

A

Y
|
Y

I

V

P

P

F

Y

G

G

D

R

D

D

W

W

F

Y

G

P

Y

Y

F

L

M

T

R

R

L

I

A

A

E

E

R

R

P

P

binding flavin-adenine dinucleotide: D139 (= D132), M140 (= M133), V167 (= V159), R190 (= R180), V192 (= V182), K193 (= K183), G194 (= G184), A195 (= A185), A231 (≠ G221), T232 (= T222), H233 (= H223), D234 (= D224), L260 (= L250), V263 (≠ F253), Y281 (= Y271)

5m42A Structure of thermus thermophilus l-proline dehydrogenase lacking alpha helices a, b and c (see paper)
40% identity, 70% coverage: 39:275/341 of query aligns to 3:242/242 of 5m42A

query
sites
5m42A

T

T

V

L

E

E

S

E

V

A

M

L

E

K

A

A

V

A

K

E

E

A

L

L

N

E

H

R

Q

E

G

G

I

V

S

H

C

A

T

I

L

L

D

D

N

L

L

L

G

G

E

E

F

M

V

V

S

R

D

T

K

E

T

E

E

E

A

A

G

R

E

A

A

F

K

Q

D

R

K

G

I

L

R

E

L

L

L

V

N

W

T

A

I

L

H

A

E

G

E

K

N

P

V

W

D

P

C

K

H

Y

L

I

S

S

V

L

K

K

L

L

T

T

Q

Q

L

L

G

G

L

L

D

D

I

L

D

S

Q

E

A

D

Y

L

C

A

T

L

E

A

N

L

M

L

R

R

E

E

I

V

L

L

D

R

I

E

A

A

R

E

Q

P

Y

R

N

G

I

V

F

F

V

V

N

R

I

L

D
|
D

M
|
M

E

E

D

D

Y

S

S

P

H

R

Y

V

D

E

Q

A

T

T

L

L

E

R

V

L

L

Y

Q

R

A

A

L

L

R

R

K

E

D

E

-

G

Y

F

D

S

N

Q

V

V

G

G

T

I

V
|
V

I

L

Q
|
Q

S

S

Y

Y

L

L

Y

Y

R

R

A

T

E

E

E

K

D

D

M

L

E

L

N

D

L

L

K

L

N

P

V

Y

R

R

-

P

-

N

L

L

R
|
R

I

L

V
|
V

K
|
K

G
|
G

A
|
A

Y

Y

K

R

E

E

S

P

E

K

E

E

V

V

A

A

Y

F

P

P

E

D

K

K

Q

R

D

L

I

I

D

D

R

A

N

E

F

Y

L

L

E

H

L

L

A

G

K

K

Q

L

R

A

L

L

A

K

G

E

D

G

T

L

F

Y

T

V

S

A

I

F

G
x
A

T
|
T

H
|
H

D

D

H

P

H

R

I

I

N

A

E

E

L

L

K

K

A

R

F

Y

A

T

D

E

E

A

H

M

N

G

I

I

D

P

H

R

D

S

K

R

F

F

E

E

F

F

Q

Q

M

F

L
|
L

Y

Y

G

G

F

V

R

R

N

P

D

E

M

E

Q

Q

N

R

K

R

L

L

A

A

N

R

E

E

G

G

Y

Y

N

T

F

V

C

R

T

A

Y

Y

I

V

P

P

F

Y

G

G

binding flavin mononucleotide: D96 (= D132), M97 (= M133), V124 (= V159), Q126 (= Q161), R147 (= R180), V149 (= V182), K150 (= K183), G151 (= G184), A152 (= A185), A188 (≠ G221), T189 (= T222), H190 (= H223), L217 (= L250)

5ur2B Crystal structure of proline utilization a (puta) from bdellovibrio bacteriovorus inactivated by n-propargylglycine (see paper)
27% identity, 77% coverage: 31:292/341 of query aligns to 90:402/959 of 5ur2B

query
sites
5ur2B

A

A

D

K

K

M

F

F

V

I

A

T

G

G

T

E

T

S

V

P

E

D

S

E

V

A

M

L

E

P

A

V

V

L

K

K

E

K

L

A

N

R

H

K

Q

N

G

K

I

M

S

T

C

F

T

T

L

V

D

D

N

I

L

L

G

G

E

E

F

A

V

T

S

L

D

S

K

E

T

K

E

E

A

A

G

Q

E

D

A

Y

K

S

D

N

K

K

I

Y

I

M

R

E

L

L

L

V

N

T

T

W

I

L

-

A

-

K

-

D

-

A

-

E

-

K

-

W

-

D

-

E

-

V

-

P

-

Q

-

I

-

D

-

R

-

D

H

H

E

E

E

G

N

A

V

L

D

P

-

K

C

V

H

N

L

V

S

S

V

V

K
|
K

L

M

T

T

Q

A

L

L

G

Y

L

S

D

Q

I

I

D

K

Q

D

A

A

Y

A

C

W

T

D

E

E

N

S

-

K

-

I

-

L

-

K

-

D

-

R

M

L

R

R

E

P

I

V

L

F

D

R

I

L

A

G

R

M

Q

E

Y

K

N

G

I

V

F

F

V

V

N

N

I

L

D
|
D

M
|
M

E

E

D

Q

Y

Y

S

S

H

V

Y

K

D

H

Q

L

T

T

L

L

E

E

V

V

L

F

Q

T

A

E

L

L

R

I

-

N

-

E

-

P

-

E

-

F

K

K

D

N

Y

Y

D

K

N

F

V

F

G

G

T

I

V

V

I

I

Q
|
Q

S

A

Y

Y

L

L

Y

R

R

D

A

S

E

F

E

E

D

D

M

V

E

K

N

S

L

L

K

T

N

E

V

F

R

A

-

Q

-

K

-

R

-

G

-

T

-

P

-

F

-

W

L

V

R

R

I

L

V
|
V

K
|
K

G
|
G

A
|
A

Y

Y

K
x
W

-

D

-

Y

-

E

-

T

-

I

E

E

S

A

E

E

E

Q

V

R

A

G

Y

W

P

P

-

V

-

P

-

V

-
x
Y

-
x
T

E
x
N

K
|
K

Q

A

D

E

I
x
S

D

D

R

A

N

N

F

Y

L

E

E

L

L

C

A

A

K

K

Q

Y

R

L

L

L

A

E

G

N

D

I

T

K

F

F

-

I

-

R

T

P

S

A

I

F

G
x
A

T
x
S

H
|
H

D
x
N

H

V

H

R

I

T

I

L

N

A

E

A

L

C

K

M

A

L

F

Y

A

A

D

E

E

K

H

L

N

N

I

I

D

P

H

K

D

E

K

A

F

L

E

E

F

F

Q
|
Q

M
|
M

L
|
L

Y

Y

G

G

F

M

R

A

N

E

D

P

M

I

Q

K

N

K

K

T

L

I

A

V

N

D

E

M

G

G

Y

Y

N

R

F

M

C

R

T

E

Y
|
Y

I

A

P

P

F

V

G

G

D

E

-

L

-

I

D

P

W

G

F

M

G

A

Y

Y

F

L

M

V

R

R

L

L

A

L

E
|
E

R

N

P

T

Q

S

N

N

binding N-propargylglycine-modified flavin adenine dinucleotide: K174 (= K98), D215 (= D132), M216 (= M133), Q249 (= Q161), V278 (= V182), K279 (= K183), G280 (= G184), A281 (= A185), W283 (≠ K187), Y300 (vs. gap), T301 (vs. gap), N302 (≠ E196), K303 (= K197), S306 (≠ I200), A329 (≠ G221), S330 (≠ T222), H331 (= H223), N332 (≠ D224), Q356 (= Q248), M357 (= M249), L358 (= L250), Y379 (= Y271), E398 (= E288)

Sites not aligning to the query:

active site: 618, 641, 722, 756, 851, 931
binding N-propargylglycine-modified flavin adenine dinucleotide: 403, 405

7na0A Structure of geobacter sulfurreducens proline utilization a (puta) variant a206w (see paper)
26% identity, 82% coverage: 31:310/341 of query aligns to 98:427/981 of 7na0A

query
sites
7na0A

A

A

D

R

K

Q

F

F

V

I

A

V

G

G

T

E

T

T

V

T

E

K

S

E

V

A

M

V

E

K

A

N

V

L

K

E

E

K

L

L

N

R

H

K

Q

D

G

G

I

F

S

A

C

A

T

V

L

V

D

D

N

V

L

L

G

G

E

E

F

A

V

T

S

L

D

S

K

E

T

E

E

E

A

A

G

E

E

V

A

Y

K

T

D

N

K

T

I

Y

I

L

R

E

L

L

N

E

T

A

I

L

H

K

E

K

E

E

N

Q

V

G

D

S

C

W

H

K

-

G

L

L

S

P

V

G

K

K

L

G

T

G

Q

D

L

P

G

G

L

L

D

D

I

W

D

G

Q

H

A

A

-

P

-

K

-

V

-

N

-

I

-

A

-

V

-

K

-

P

-

T

-

W

-

L

Y

F

C

C

T

L

E

A

N

N

-

P

-

Q

-

D

-

F

-

E

-

G

-

S

-

V

-

A

-

I

-

L

-

D

-

R

M

M

R

R

E

R

I

I

L

F

D

K

I

K

A

V

R

M

Q

E

Y

L

N

N

I

G

F

F

V

L

N

C

I

I

D
|
D

M
|
M

E

E

D

S

Y

Y

S

R

H

H

Y

K

D

E

Q

I

T

I

L

L

E

E

V

V

L

F

Q

R

A

R

L

L

R

K

-

L

-

E

-

Y

K

R

D

D

Y

Y

D

P

N

H

V

L

G

G

T

I

V
|
V

I

L

Q
|
Q

S

A

Y

Y

L

L

Y

K

R

D

A

N

E

D

E

K

D

D

M

L

-

D

-

L

-

A

-

W

-

A

-

K

E

E

N

H

L

K

K

V

N

Q

V

I

R

S

L

V

R
|
R

I

L

V
|
V

K
|
K

G
|
G

A
|
A

Y
|
Y

K
x
W

E

D

S

Y

E

E

E

T

V

V

-

K

-

A

-

K

-

Q

-

N

-

D

-

W

-

E

-

V

-

P

A

V

Y
x
W

P
x
T

E
x
I

K
|
K

Q

A

D

E

I
x
S

D

D

R

A

N

A

F

Y

L

E

E

R

L

Q

A

A

K

R

Q

K

R

I

L

L

A

E

G

N

D

H

T

Q

F

I

T

C

S

H

I

F

G

A

T

C

H
x
A

D
x
S

H
|
H

H
x
N

I

I

-

R

-

T

I

I

N

S

E

A

L

V

K

M

A

E

F

M

A

A

D

R

E

E

H

L

N

N

I

V

D

P

H

E

D

D

K

R

F

Y

E

E

F

F

Q

Q

M

V

L
|
L

Y

Y

G

G

F

M

R

A

N

E

D

P

M

V

Q

R

N

K

K

G

L

I

A

L

N

K

E

V

G

A

Y

G

N

R

F

I

C

R

T

L

Y
|
Y

I

A

P

P

F

Y

G

G

D

N

-

M

-

V

D

P

W

G

F

M

G

G

Y

Y

F

L

M

V

R

R

L

L

A

L

E
|
E

R

N

P

T

Q

A

N

N

M

E

T
x
S

L
x
F

V

-

L

L

K

R

D

Q

T

S

F

F

Y

-

T

A

E

E

D

D

N

A

K

Q

L

I

K

E

K

R

binding flavin-adenine dinucleotide: D226 (= D132), M227 (= M133), V256 (= V159), Q258 (= Q161), R285 (= R180), V287 (= V182), K288 (= K183), G289 (= G184), A290 (= A185), Y291 (= Y186), W292 (≠ K187), W309 (≠ Y194), T310 (≠ P195), I311 (≠ E196), K312 (= K197), S315 (≠ I200), A338 (≠ H223), S339 (≠ D224), H340 (= H225), N341 (≠ H226), L367 (= L250), Y388 (= Y271), E407 (= E288), S413 (≠ T294), F414 (≠ L295)

4nmfB Crystal structure of proline utilization a (puta) from geobacter sulfurreducens pca inactivated by n-propargylglycine and complexed with menadione bisulfite (see paper)
26% identity, 82% coverage: 31:310/341 of query aligns to 97:426/979 of 4nmfB

query
sites
4nmfB

A

A

D

R

K

Q

F

F

V

I

A

V

G

G

T

E

T

T

V

T

E

K

S

E

V

A

M

V

E

K

A

N

V

L

K

E

E

K

L

L

N

R

H

K

Q

D

G

G

I

F

S

A

C

A

T

V

L

V

D

D

N

V

L

L

G

G

E

E

F

A

V

T

S

L

D

S

K

E

T

E

E

E

A

A

G

E

E

V

A

Y

K

T

D

N

K

T

I

Y

I

L

R

E

L

L

N

E

T

A

I

L

H

K

E

K

E

E

N

Q

V

G

D

S

C

W

H

K

-

G

L

L

S

P

V

G

K

K

L

G

T

G

Q

D

L

P

G

G

L

L

D

D

I

W

D

G

Q

H

A

A

-

P

-

K

-

V

-

N

-

I

-

A

-

V

-
x
K

-

P

-

T

-

A

-

L

Y

F

C

C

T

L

E

A

N

N

-

P

-

Q

-

D

-

F

-

E

-

G

-

S

-

V

-

A

-

I

-

L

-

D

-

R

M

M

R

R

E

R

I

I

L

F

D

K

I

K

A

V

R

M

Q

E

Y

L

N

N

I

G

F

F

V

L

N

C

I

I

D
|
D

M
|
M

E

E

D

S

Y

Y

S

R

H

H

Y

K

D

E

Q

I

T

I

L

L

E

E

V

V

L

F

Q

R

A

R

L

L

R

K

-

L

-

E

-

Y

K

R

D

D

Y

Y

D

P

N

H

V

L

G

G

T

I

V
|
V

I

L

Q
|
Q

S

A

Y

Y

L

L

Y

K

R

D

A

N

E

D

E

K

D

D

M

L

-

D

-

L

-

A

-

W

-

A

-

K

E

E

N

H

L

K

K

V

N

Q

V

I

R

S

L

V

R
|
R

I

L

V
|
V

K
|
K

G
|
G

A
|
A

Y
|
Y

K
x
W

E

D

S

Y

E

E

E

T

V

V

-

K

-

A

-

K

-

Q

-

N

-

D

-

W

-

E

-

V

-

P

A

V

Y
x
W

P
x
T

E
x
I

K
|
K

Q

A

D

E

I
x
S

D

D

R

A

N

A

F

Y

L

E

E

R

L

Q

A

A

K

R

Q

K

R

I

L

L

A

E

G

N

D

H

T

Q

F

I

T

C

S

H

I

F

G

A

T

C

H
x
A

D
x
S

H
|
H

H
x
N

I

I

-

R

-

T

I

I

N

S

E

A

L

V

K

M

A

E

F

M

A

A

D

R

E

E

H

L

N

N

I

V

D

P

H

E

D

D

K

R

F

Y

E

E

F

F

Q
|
Q

M

V

L
|
L

Y

Y

G

G

F

M

R

A

N

E

D

P

M

V

Q

R

N

K

K

G

L

I

A

L

N

K

E

V

G

A

Y

G

N

R

F

I

C

R

T

L

Y
|
Y

I

A

P

P

F

Y

G

G

D

N

-

M

-

V

D

P

W

G

F

M

G

G

Y
|
Y

F

L

M

V

R
|
R

L

L

A

L

E
|
E

R

N

P

T

Q

A

N

N

M
x
E

T
x
S

L
x
F

V

-

L

L

K

R

D

Q

T

S

F

F

Y

-

T

A

E

E

D

D

N

A

K

Q

L

I

K

E

K

R

binding (2R)-2-methyl-1,4-dioxo-1,2,3,4-tetrahydronaphthalene-2-sulfonic acid: K184 (vs. gap), Y290 (= Y186), Y387 (= Y271), Y399 (= Y281), R402 (= R284), R403 (= R285)
binding (2S)-2-methyl-1,4-dioxo-1,2,3,4-tetrahydronaphthalene-2-sulfonic acid: K184 (vs. gap), L366 (= L250), Y399 (= Y281), R402 (= R284)
binding N-propargylglycine-modified flavin adenine dinucleotide: K184 (vs. gap), D225 (= D132), M226 (= M133), V255 (= V159), Q257 (= Q161), R284 (= R180), V286 (= V182), K287 (= K183), G288 (= G184), A289 (= A185), W291 (≠ K187), W308 (≠ Y194), T309 (≠ P195), I310 (≠ E196), K311 (= K197), S314 (≠ I200), A337 (≠ H223), S338 (≠ D224), H339 (= H225), N340 (≠ H226), Q364 (= Q248), L366 (= L250), Y387 (= Y271), E406 (= E288), E411 (≠ M293), S412 (≠ T294), F413 (≠ L295)

Sites not aligning to the query:

active site: 631, 654, 735, 769, 865, 945

4nmdA Crystal structure of proline utilization a (puta) from geobacter sulfurreducens pca reduced with dithionite (see paper)
26% identity, 82% coverage: 31:310/341 of query aligns to 98:427/979 of 4nmdA

query
sites
4nmdA

A

A

D

R

K

Q

F

F

V

I

A

V

G

G

T

E

T

T

V

T

E

K

S

E

V

A

M

V

E

K

A

N

V

L

K

E

E

K

L

L

N

R

H

K

Q

D

G

G

I

F

S

A

C

A

T

V

L

V

D

D

N

V

L

L

G

G

E

E

F

A

V

T

S

L

D

S

K

E

T

E

E

E

A

A

G

E

E

V

A

Y

K

T

D

N

K

T

I

Y

I

L

R

E

L

L

N

E

T

A

I

L

H

K

E

K

E

E

N

Q

V

G

D

S

C

W

H

K

-

G

L

L

S

P

V

G

K

K

L

G

T

G

Q

D

L

P

G

G

L

L

D

D

I

W

D

G

Q

H

A

A

-

P

-

K

-

V

-

N

-

I

-

A

-

V

-

K

-

P

-

T

-

A

-

L

Y

F

C

C

T

L

E

A

N

N

-

P

-

Q

-

D

-

F

-

E

-

G

-

S

-

V

-

A

-

I

-

L

-

D

-

R

M

M

R

R

E

R

I

I

L

F

D

K

I

K

A

V

R

M

Q

E

Y

L

N

N

I

G

F

F

V

L

N

C

I

I

D
|
D

M
|
M

E

E

D

S

Y

Y

S

R

H

H

Y

K

D

E

Q

I

T

I

L

L

E

E

V

V

L

F

Q

R

A

R

L

L

R

K

-

L

-

E

-

Y

K

R

D

D

Y

Y

D

P

N

H

V

L

G

G

T

I

V
|
V

I

L

Q
|
Q

S

A

Y

Y

L

L

Y

K

R

D

A

N

E

D

E

K

D

D

M

L

-

D

-

L

-

A

-

W

-

A

-

K

E

E

N

H

L

K

K

V

N

Q

V

I

R

S

L

V

R
|
R

I

L

V
|
V

K
|
K

G
|
G

A
|
A

Y

Y

K
x
W

E

D

S

Y

E

E

E

T

V

V

-

K

-

A

-

K

-

Q

-

N

-

D

-

W

-

E

-

V

-

P

A

V

Y
x
W

P
x
T

E
x
I

K
|
K

Q

A

D

E

I
x
S

D

D

R

A

N

A

F

Y

L

E

E

R

L

Q

A

A

K

R

Q

K

R

I

L

L

A

E

G

N

D

H

T

Q

F

I

T

C

S

H

I

F

G

A

T

C

H
x
A

D
x
S

H
|
H

H
x
N

I

I

-

R

-

T

I

I

N

S

E

A

L

V

K

M

A

E

F

M

A

A

D

R

E

E

H

L

N

N

I

V

D

P

H

E

D

D

K

R

F

Y

E

E

F

F

Q
|
Q

M
x
V

L
|
L

Y

Y

G

G

F

M

R

A

N

E

D

P

M

V

Q

R

N

K

K

G

L

I

A

L

N

K

E

V

G

A

Y

G

N

R

F

I

C

R

T

L

Y
|
Y

I

A

P

P

F

Y

G

G

D

N

-

M

-

V

D

P

W

G

F

M

G

G

Y

Y

F

L

M

V

R

R

L

L

A

L

E

E

R

N

P

T

Q

A

N

N

M

E

T

S

L
x
F

V

-

L

L

K

R

D

Q

T

S

F

F

Y

-

T

A

E

E

D

D

N

A

K

Q

L

I

K

E

K

R

binding dihydroflavine-adenine dinucleotide: D226 (= D132), M227 (= M133), V256 (= V159), Q258 (= Q161), R285 (= R180), V287 (= V182), K288 (= K183), G289 (= G184), A290 (= A185), W292 (≠ K187), W309 (≠ Y194), T310 (≠ P195), I311 (≠ E196), K312 (= K197), S315 (≠ I200), A338 (≠ H223), S339 (≠ D224), H340 (= H225), N341 (≠ H226), Q365 (= Q248), V366 (≠ M249), L367 (= L250), Y388 (= Y271), F414 (≠ L295)

Sites not aligning to the query:

active site: 631, 654, 735, 769, 865, 945

4nmaA Crystal structure of proline utilization a (puta) from geobacter sulfurreducens pca in complex with l-tetrahydro-2-furoic acid (see paper)
26% identity, 82% coverage: 31:310/341 of query aligns to 98:427/977 of 4nmaA

query
sites
4nmaA

A

A

D

R

K

Q

F

F

V

I

A

V

G

G

T

E

T

T

V

T

E

K

S

E

V

A

M

V

E

K

A

N

V

L

K

E

E

K

L

L

N

R

H

K

Q

D

G

G

I

F

S

A

C

A

T

V

L

V

D

D

N

V

L

L

G

G

E

E

F

A

V

T

S

L

D

S

K

E

T

E

E

E

A

A

G

E

E

V

A

Y

K

T

D

N

K

T

I

Y

I

L

R

E

L

L

N

E

T

A

I

L

H

K

E

K

E

E

N

Q

V

G

D

S

C

W

H

K

-

G

L

L

S

P

V

G

K

K

L

G

T

G

Q

D

L

P

G

G

L

L

D

D

I

W

D

G

Q

H

A

A

-

P

-

K

-

V

-

N

-

I

-

A

-

V

-
x
K

-

P

-

T

-

A

-

L

Y

F

C

C

T

L

E

A

N

N

-

P

-

Q

-

D

-

F

-

E

-

G

-

S

-

V

-

A

-

I

-

L

-

D

-

R

M

M

R

R

E

R

I

I

L

F

D

K

I

K

A

V

R

M

Q

E

Y

L

N

N

I

G

F

F

V

L

N

C

I

I

D
|
D

M
|
M

E

E

D

S

Y

Y

S

R

H

H

Y

K

D

E

Q

I

T

I

L

L

E

E

V

V

L

F

Q

R

A

R

L

L

R

K

-

L

-

E

-

Y

K

R

D

D

Y

Y

D

P

N

H

V

L

G

G

T

I

V

V

I

L

Q
|
Q

S

A

Y

Y

L

L

Y

K

R

D

A

N

E

D

E

K

D

D

M

L

-

D

-

L

-

A

-

W

-

A

-

K

E

E

N

H

L

K

K

V

N

Q

V

I

R

S

L

V

R
|
R

I

L

V
|
V

K
|
K

G
|
G

A
|
A

Y
|
Y

K
x
W

E

D

S

Y

E

E

E

T

V

V

-

K

-

A

-

K

-

Q

-

N

-

D

-

W

-

E

-

V

-

P

A

V

Y
x
W

P
x
T

E
x
I

K
|
K

Q

A

D

E

I
x
S

D

D

R

A

N

A

F

Y

L

E

E

R

L

Q

A

A

K

R

Q

K

R

I

L

L

A

E

G

N

D

H

T

Q

F

I

T

C

S

H

I

F

G

A

T

C

H
x
A

D
x
S

H
|
H

H
x
N

I

I

-

R

-

T

I

I

N

S

E

A

L

V

K

M

A

E

F

M

A

A

D

R

E

E

H

L

N

N

I

V

D

P

H

E

D

D

K

R

F

Y

E

E

F

F

Q
|
Q

M

V

L
|
L

Y

Y

G

G

F

M

R

A

N

E

D

P

M

V

Q

R

N

K

K

G

L

I

A

L

N

K

E

V

G

A

Y

G

N

R

F

I

C

R

T

L

Y
|
Y

I

A

P

P

F

Y

G

G

D

N

-

M

-

V

D

P

W

G

F

M

G

G

Y
|
Y

F

L

M

V

R
|
R

L

L

A

L

E
|
E

R

N

P

T

Q

A

N

N

M

E

T
x
S

L
x
F

V

-

L

L

K

R

D

Q

T

S

F

F

Y

-

T

A

E

E

D

D

N

A

K

Q

L

I

K

E

K

R

binding flavin-adenine dinucleotide: D226 (= D132), M227 (= M133), Q258 (= Q161), R285 (= R180), V287 (= V182), K288 (= K183), G289 (= G184), A290 (= A185), Y291 (= Y186), W292 (≠ K187), W309 (≠ Y194), T310 (≠ P195), I311 (≠ E196), K312 (= K197), S315 (≠ I200), A338 (≠ H223), S339 (≠ D224), H340 (= H225), N341 (≠ H226), Q365 (= Q248), L367 (= L250), E407 (= E288), S413 (≠ T294), F414 (≠ L295)
binding tetrahydrofuran-2-carboxylic acid: K185 (vs. gap), Y388 (= Y271), Y400 (= Y281), R403 (= R284), R404 (= R285)

Sites not aligning to the query:

active site: 629, 652, 733, 767, 863, 943

4nmfA Crystal structure of proline utilization a (puta) from geobacter sulfurreducens pca inactivated by n-propargylglycine and complexed with menadione bisulfite (see paper)
26% identity, 79% coverage: 31:301/341 of query aligns to 94:415/973 of 4nmfA

query
sites
4nmfA

A

A

D

R

K

Q

F

F

V

I

A

V

G

G

T

E

T

T

V

T

E

K

S

E

V

A

M

V

E

K

A

N

V

L

K

E

E

K

L

L

N

R

H

K

Q

D

G

G

I

F

S

A

C

A

T

V

L

V

D

D

N

V

L

L

G

G

E

E

F

A

V

T

S

L

D

S

K

E

T

E

E

E

A

A

G

E

E

V

A

Y

K

T

D

N

K

T

I

Y

I

L

R

E

L

L

N

E

T

A

I

L

H

K

E

K

E

E

N

Q

V

G

D

S

C

W

H

K

-

G

L

L

S

P

V

G

K

K

L

G

T

G

Q

D

L

P

G

G

L

L

D

D

I

W

D

G

Q

H

A

A

-

P

-

K

-

V

-

N

-

I

-

A

-

V

-
x
K

-

P

-

T

-

A

-

L

Y

F

C

C

T

L

E

A

N

N

-

P

-

Q

-

D

-

F

-

E

-

G

-

S

-

V

-

A

-

I

-

L

-

D

-

R

M

M

R

R

E

R

I

I

L

F

D

K

I

K

A

V

R

M

Q

E

Y

L

N

N

I

G

F

F

V

L

N

C

I

I

D
|
D

M
|
M

E

E

D

S

Y

Y

S

R

H

H

Y

K

D

E

Q

I

T

I

L

L

E

E

V

V

L

F

Q

R

A

R

L

L

R

K

-

L

-

E

-

Y

K

R

D

D

Y

Y

D

P

N

H

V

L

G

G

T

I

V
|
V

I

L

Q
|
Q

S

A

Y

Y

L

L

Y

K

R

D

A

N

E

D

E

K

D

D

M

L

-

D

-

L

-

A

-

W

-

A

-

K

E

E

N

H

L

K

K

V

N

Q

V

I

R

S

L

V

R
|
R

I

L

V
|
V

K
|
K

G
|
G

A
|
A

Y
|
Y

K
x
W

E

D

S

Y

E

E

E

T

V

V

-

K

-

A

-

K

-

Q

-

N

-

D

-

W

-

E

-

V

-

P

A

V

Y
x
W

P
x
T

E
x
I

K
|
K

Q

A

D

E

I
x
S

D

D

R

A

N

A

F

Y

L

E

E

R

L

Q

A

A

K

R

Q

K

R

I

L

L

A

E

G

N

D

H

T

Q

F

I

T

C

S

H

I

F

G

A

T

C

H
x
A

D
x
S

H
|
H

H
x
N

I

I

-

R

-

T

I

I

N

S

E

A

L

V

K

M

A

E

F

M

A

A

D

R

E

E

H

L

N

N

I

V

D

P

H

E

D

D

K

R

F

Y

E

E

F

F

Q
|
Q

M
x
V

L
|
L

Y

Y

G

G

F

M

R

A

N

E

D

P

M

V

Q

R

N

K

K

G

L

I

A

L

N

K

E

V

G

A

Y

G

N

R

F

I

C

R

T

L

Y
|
Y

I

A

P

P

F

Y

G

G

D

N

-

M

-

V

D

P

W

G

F

M

G

G

Y
|
Y

F

L

M

V

R
|
R

L

L

A

L

E
|
E

R

N

P

T

Q

A

N

N

M
x
E

T

S

L
x
F

V

-

L

L

K

R

D

Q

T

S

F

F

binding (2R)-2-methyl-1,4-dioxo-1,2,3,4-tetrahydronaphthalene-2-sulfonic acid: K181 (vs. gap), Y287 (= Y186), Y384 (= Y271), Y396 (= Y281), R399 (= R284), R400 (= R285)
binding N-propargylglycine-modified flavin adenine dinucleotide: K181 (vs. gap), D222 (= D132), M223 (= M133), V252 (= V159), Q254 (= Q161), R281 (= R180), V283 (= V182), K284 (= K183), G285 (= G184), A286 (= A185), W288 (≠ K187), W305 (≠ Y194), T306 (≠ P195), I307 (≠ E196), K308 (= K197), S311 (≠ I200), A334 (≠ H223), S335 (≠ D224), H336 (= H225), N337 (≠ H226), Q361 (= Q248), V362 (≠ M249), L363 (= L250), Y384 (= Y271), E403 (= E288), E408 (≠ M293), F410 (≠ L295)

Sites not aligning to the query:

active site: 625, 648, 729, 763, 859, 939

4nmeA Crystal structure of proline utilization a (puta) from geobacter sulfurreducens pca inactivated by n-propargylglycine (see paper)
27% identity, 82% coverage: 31:310/341 of query aligns to 96:422/972 of 4nmeA

query
sites
4nmeA

A

A

D

R

K

Q

F

F

V

I

A

V

G

G

T

E

T

T

V

T

E

K

S

E

V

A

M

V

E

K

A

N

V

L

K

E

E

K

L

L

N

R

H

K

Q

D

G

G

I

F

S

A

C

A

T

V

L

V

D

D

N

V

L

L

G

G

E

E

F

A

V

T

S

L

D

S

K

E

T

E

E

E

A

A

G

E

E

V

A

Y

K

T

D

N

K

T

I

Y

I

L

R

E

L

L

N

E

T

A

I

L

H

K

E

K

E

E

N

Q

V

G

D

S

C

W

H

K

-

G

L

L

S

P

V

G

K

K

L

G

T

G

Q

D

L

P

G

G

L

L

D

D

I

W

D

G

Q

H

A

A

-

P

-

K

-

V

-

N

-

I

-

A

-

V

-
x
K

-

P

-

T

-

A

-

L

Y

F

C

C

T

L

E

A

N

N

-

P

-

Q

-

D

-

F

-

E

-

G

-

S

-

V

-

A

-

I

-

L

-

D

-

R

M

M

R

R

E

R

I

I

L

F

D

K

I

K

A

V

R

M

Q

E

Y

L

N

N

I

G

F

F

V

L

N

C

I

I

D
|
D

M
|
M

E

E

D

S

Y

Y

S

R

H

H

Y

K

D

E

Q

I

T

I

L

L

E

E

V

V

L

F

Q

R

A

R

L

L

R

K

-

L

-

E

-

Y

K

R

D

D

Y

Y

D

P

N

H

V

L

G

G

T

I

V
|
V

I

L

Q
|
Q

S

A

Y

Y

L

L

Y

K

R

D

A

N

E

D

E

K

D

D

M

L

-

D

-

L

-

A

-

W

-

A

-

K

E

E

N

H

L

K

K

V

N

Q

V

I

R

S

L

V

R
|
R

I

L

V
|
V

K
|
K

G
|
G

A
|
A

Y

Y

K
x
W

E

D

S

Y

E

E

E

T

V

V

-

K

-

A

-

K

-

Q

-

N

-

D

-

W

-

E

-

V

-

P

A

V

Y
x
W

P
x
T

E
x
I

K
|
K

Q

A

D

E

I
x
S

D

D

R

A

N

A

F

Y

L

E

E

R

L

Q

A

A

K

R

Q

K

R

I

L

L

A

E

G

N

D

H

T

Q

F

I

T

C

S

H

I

F

G

A

T

C

H
x
A

D
x
S

H
|
H

H
x
N

I

I

-

R

-

T

I

I

N

S

E

A

L

V

K

M

A

E

F

M

A

A

D

R

E

E

H

L

N

N

I

V

D

P

H

E

D

D

K

R

F

Y

E

E

F

F

Q
|
Q

M

V

L
|
L

Y

Y

G

G

F

M

R

V

N

R

D

K

M

G

Q

I

N

L

K

K

L

V

A

A

N

G

E

-

G

-

Y

-

N

R

F

I

C

R

T

L

Y
|
Y

I

A

P

P

F

Y

G

G

D

N

-

M

-

V

D

P

W

G

F

M

G

G

Y

Y

F

L

M

V

R

R

L

L

A

L

E
|
E

R

N

P

T

Q

A

N

N

M

E

T

S

L
x
F

V

-

L

L

K

R

D

Q

T

S

F

F

Y

-

T

A

E

E

D

D

N

A

K

Q

L

I

K

E

K

R

binding N-propargylglycine-modified flavin adenine dinucleotide: K183 (vs. gap), D224 (= D132), M225 (= M133), V254 (= V159), Q256 (= Q161), R283 (= R180), V285 (= V182), K286 (= K183), G287 (= G184), A288 (= A185), W290 (≠ K187), W307 (≠ Y194), T308 (≠ P195), I309 (≠ E196), K310 (= K197), S313 (≠ I200), A336 (≠ H223), S337 (≠ D224), H338 (= H225), N339 (≠ H226), Q363 (= Q248), L365 (= L250), Y383 (= Y271), E402 (= E288), F409 (≠ L295)

Sites not aligning to the query:

active site: 624, 647, 728, 762, 858, 938

4nmcA Crystal structure of oxidized proline utilization a (puta) from geobacter sulfurreducens pca complexed with zwittergent 3-12 (see paper)
26% identity, 76% coverage: 31:290/341 of query aligns to 87:397/941 of 4nmcA

query
sites
4nmcA

A

A

D

R

K

Q

F

F

V

I

A

V

G

G

T

E

T

T

V

T

E

K

S

E

V

A

M

V

E

K

A

N

V

L

K

E

E

K

L

L

N

R

H

K

Q

D

G

G

I

F

S

A

C

A

T

V

L

V

D

D

N

V

L

L

G

G

E

E

F

A

V

T

S

L

D

S

K

E

T

E

E

E

A

A

G

E

E

V

A

Y

K

T

D

N

K

T

I

Y

I

L

R

E

L

L

N

E

T

A

I

L

H

K

E

K

E

E

N

Q

V

G

D

S

C

W

H

K

-

G

L

L

S

P

V

G

K

K

L

G

T

G

Q

D

L

P

G

G

L

L

D

D

I

W

D

G

Q

H

A

A

-

P

-

K

-

V

-

N

-

I

-

A

-

V

-

K

-

P

-

T

-

A

-

L

Y

F

C

C

T

L

E

A

N

N

-

P

-

Q

-

D

-

F

-

E

-

G

-

S

-

V

-

A

-

I

-

L

-

D

-

R

M

M

R

R

E

R

I

I

L

F

D

K

I

K

A

V

R

M

Q

E

Y

L

N

N

I

G

F

F

V

L

N

C

I

I

D
|
D

M
|
M

E

E

D

S

Y

Y

S

R

H

H

Y

K

D

E

Q

I

T

I

L

L

E

E

V

V

L

F

Q

R

A

R

L

L

R

K

-

L

-

E

-

Y

K

R

D

D

Y

Y

D

P

N

H

V

L

G

G

T

I

V
|
V

I

L

Q
|
Q

S

A

Y

Y

L

L

Y

K

R

D

A

N

E

D

E

K

D

D

M

L

-

D

-

L

-

A

-

W

-

A

-

K

E

E

N

H

L

K

K

V

N

Q

V

I

R

S

L

V

R
|
R

I

L

V
|
V

K
|
K

G
|
G

A
|
A

Y

Y

K
x
W

E

D

S

Y

E

E

E

T

V

V

-

K

-

A

-

K

-

Q

-

N

-

D

-

W

-

E

-

V

-

P

A

V

Y
x
W

P
x
T

E
x
I

K
|
K

Q

A

D

E

I
x
S

D

D

R

A

N

A

F

Y

L

E

E

R

L

Q

A

A

K

R

Q

K

R

I

L

L

A

E

G

N

D

H

T

Q

F

I

T

C

S

H

I

F

G

A

T

C

H
x
A

D
x
S

H
|
H

H
x
N

I

I

-

R

-

T