SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_010531996.1 NCBI__GCF_000224785.1:WP_010531996.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q8NMB0 Vanillin dehydrogenase; Aromatic aldehyde dehydrogenase; EC 1.2.1.67; EC 1.2.1.64; EC 1.2.1.96 from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) (see paper)
48% identity, 96% coverage: 9:476/485 of query aligns to 12:481/484 of Q8NMB0

query
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Q8NMB0

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N157 (= N154) mutation to A: Less than 50% of the activity of the wild-type with vanillin as substrate in the presence of NAD(+). Less than 10% of the activity of the wild-type with vanillin as substrate in the presence of NADP(+). 4.5-fold decreased affinity for NAD(+), 11-fold decreased affinity for NADP(+) and 2.3-fold decreased affinity for vanillin compared to the wild type.
K180 (= K177) mutation to A: Less than 50% of the activity of the wild-type with vanillin as substrate in the presence of NAD(+). Less than 10% of the activity of the wild-type with vanillin as substrate in the presence of NADP(+). 4.5-fold decreased affinity for NAD(+), 11-fold decreased affinity for NADP(+) and 5-fold decreased affinity for vanillin compared to the wild type.
E199 (= E196) mutation to A: Less than 50% of the activity of the wild-type with vanillin as substrate in the presence of NAD(+). 78% of the activity of the wild-type with vanillin as substrate in the presence of NADP(+). 5-fold decreased affinity for NAD(+), 2.5-fold decreased affinity for NADP(+) and 1.5-fold decreased affinity for vanillin compared to the wild type.
E258 (= E253) mutation to A: Less than 50% of the activity of the wild-type with vanillin as substrate in the presence of NAD(+). 24% of the activity of the wild-type with vanillin as substrate in the presence of NADP(+). 3.5-fold decreased affinity for NAD(+), 5-fold decreased affinity for NADP(+) and 3.7-fold decreased affinity for vanillin compared to the wild type.
C292 (= C287) mutation to A: Less than 50% of the activity of the wild-type with vanillin as substrate in the presence of NAD(+). Less than 10% of the activity of the wild-type with vanillin as substrate in the presence of NADP(+). 4.5-fold decreased affinity for NAD(+), 7-fold decreased affinity for NADP(+) and 8-fold decreased affinity for vanillin compared to the wild type.

5ek6A Thermostable aldehyde dehydrogenase from pyrobaculum sp. 1860 complexed with NADP and isobutyraldehyde (see paper)
37% identity, 98% coverage: 11:484/485 of query aligns to 4:477/482 of 5ek6A

query
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5ek6A

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active site: N147 (= N154), K170 (= K177), E245 (= E253), C279 (= C287), E374 (= E381), E452 (≠ L459)
binding 2-methylpropanal: I152 (≠ L159), K155 (= K162), T222 (= T230), E245 (= E253), F441 (= F448)
binding nadp nicotinamide-adenine-dinucleotide phosphate: I143 (= I150), T144 (≠ S151), W146 (≠ F153), N147 (= N154), I152 (≠ L159), K170 (= K177), A172 (≠ H179), S173 (≠ E180), P202 (≠ D210), G203 (≠ I211), G207 (= G215), F221 (= F229), T222 (= T230), G223 (= G231), E224 (≠ S232), T227 (≠ V235), I231 (= I239), E245 (= E253), L246 (= L254), C279 (= C287), E374 (= E381)

4h73A Thermostable aldehyde dehydrogenase from pyrobaculum sp. Complexed with NADP+
37% identity, 98% coverage: 11:484/485 of query aligns to 4:477/482 of 4h73A

query
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K

K

K

T

P

V

I

T

L

L

E
|
E

L

L

G

G

N

S

S

D

A

P

F

L

I

I

L

L

D

D

A

V

D

D

V

V

D

D

Y

Y

A

A

V

A

E

R

A

L

A

A

A

V

F

F

S

A

R

S

F

L

T

F

H

H

Q

Q

G

G

Q

Q

I

I

C
|
C

M

T

S

S

A

A

N

K

R

R

I

I

V

V

H

H

Q

K

S

A

I

V

Y

A

D

D

E

K

F

F

I

I

T

E

K

R

Y

Y

K

V

E

H

K

Y

I

V

A

K

S

M

L

L

K

R

T

I

G

D

D

D

P

P

R

R

D

K

P

D

D

E

T

K

V

V

-

D

I

L

G

G

P

P

V

L

V

I

N

N

E

E

G

R

A

Q

A

V

E

A

N

L

L

M

K

K

A

E

I

F

I

V

D

D

K

D

G

A

I

V

E

S

Q

R

G

G

A

G

T

R

P

L

I

L

L

I

K

G

G

G

D

R

I

S

S

W

G

G

N

N

M

F

V

F

E

E

P

P

T

A

I

I

L

F

T

V

D

D

V

V

T

D

S

R

D

N

M

F

A

R

V

I

A

M

Q

R

E

E

E
|
E

L

V

F

F

G

G

P

P

V

V

V

R

S

P

V

I

M

V

T

V

F

V

E

E

T

N

D

D

E

D

E

Q

A

A

I

V

K

E

I

V

A

A

N

N

D

D

T

T

E

D

F

Y

G

G

L

L

S

S

G

G

A

A

V

V

H

L

T

T

S

N

D

N

G

V

E

N

R

R

G

A

V

F

Q

R

L

I

A

A

K

E

K

A

V

V

H

E

T

S

G

G

M

M

V

F

H

H

V

I

N

N

D

D

I

V

T

T

I

F

N

L

D

E

E

E

P

S

I

H

V

V

A

P

F

F

G

G

E

I

K

K

K

A

S

S

G

G

M

V

G

G

R

R

L
x
E

N

G

G

G

D

E

W

W

S

S

L

F

E

H

E

E

F

T

T

T

T

Y

L

D

K

R

W

W

I

V

S

T

V

V

H

T

Y

L

Q

R

Q

T

R

R

E

R

I

F

P

P

active site: N147 (= N154), K170 (= K177), E245 (= E253), C279 (= C287), E374 (= E381), E452 (≠ L459)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: I143 (= I150), T144 (≠ S151), P145 (= P152), W146 (≠ F153), K170 (= K177), A172 (≠ H179), S173 (≠ E180), G203 (≠ I211), G207 (= G215), F221 (= F229), G223 (= G231), E224 (≠ S232), T227 (≠ V235)

5ekcE Thermostable aldehyde dehydrogenase from pyrobaculum sp.1860 complexed with NADP+
37% identity, 98% coverage: 11:484/485 of query aligns to 11:484/490 of 5ekcE

query
sites
5ekcE

Y

Y

I

I

A

N

G

G

T

E

W

F

R

K

D

E

G

P

R

S

S

T

N

G

N

A

T

F

L

Q

T

V

D

K

K

T

N

S

P

P

Y

V

N

D

G

G

E

S

R

K

I

I

T

A

Q

E

F

V

K

P

M

R

A

S

T

G

T

R

E

E

D

D

V

A

D

R

E

E

A

A

Y

I

K

D

S

S

A

A

Q

F

D

E

A

A

M

L

D

K

E

A

W

W

N

A

Q

N

I

I

N

P

P

A

Y

I

K

R

Q

R

R

A

D

E

T

Y

L

L

E

Y

N

K

A

M

V

L

T

E

Y

V

I

F

E

R

E

Q

H

M

E

K

E

E

D

D

I

F

T

M

D

K

I

I

I

L

I

T

D

V

E

E

L

G

G

G

T

T

R

Y

L

R

K

K

A

V

A

W

F

G

E

E

I

V

G

V

L

F

V

T

K

E

D

R

M

L

I

I

K

Q

E

N

A

A

T

E

F

L

P

A

L

R

N

H

K

Y

E

Q

G

G

K

R

I

V

L

L

P

Q

S

S

V

D

E

S

N

E

G

S

K

T

E

I

N

S

R

V

L

V

Y

F

R

K

M

R

P

S

A

K

G

G

V

V

G

G

V

V

I
|
I

S
x
T

P
|
P

F
x
W

N
|
N

F

Y

P

P

F

L

F

S

L

I

S

S

I

M

K

K

S

K

V

I

A

A

P

H

A

T

I

L

G

A

A

V

G

G

N

N

G

T

V

V

L

Y

K
|
K

A

P

H

A

E
x
S

E

D

T

T

P

P

I

V

M

T

G

G

G

-

T

W

L

L

I

I

A

A

K

Q

I

M

F

V

E

A

E

K

A

A

G

G

V

L

P

P

K

K

G

G

L

V

F

F

N

N

V

L

V

V

V

I

T

G

D

P

I
x
G

N

P

E

V

I

V

G
|
G

D

E

T

E

F

I

V

V

E

T

H

H

P

K

I

R

P

V

R

A

I

H

I

V

S

T

F
|
F

T

T

G
|
G

S
x
E

T

S

K

S

V
x
T

G

G

S

R

H

E

I

I

G

A

Q

A

L

K

A

A

V

A

K

G

H

T

F

L

K

K

K

T

P

V

I

T

L

L

E
|
E

L

L

G

G

N

S

S

D

A

P

F

L

I

I

L

L

D

D

A

V

D

D

V

V

D

D

Y

Y

A

A

V

A

E

R

A

L

A

A

A

V

F

F

S

A

R

S

F

L

T

F

H

H

Q

Q

G

G

Q

Q

I

I

C
|
C

M

T

S

S

A

A

N

K

R

R

I

I

V

V

H

H

Q

K

S

A

I

V

Y

A

D

D

E

K

F

F

I

I

T

E

K

R

Y

Y

K

V

E

H

K

Y

I

V

A

K

S

M

L

L

K

R

T

I

G

D

D

D

P

P

R

R

D

K

P

D

D

E

T

K

V

V

-

D

I

L

G

G

P

P

V

L

V

I

N
|
N

E

E

G
x
R

A
x
Q

A

V

E

A

N

L

L

M

K

K

A

E

I

F

I

V

D

D

K

D

G

A

I

V

E

S

Q

R

G

G

A

G

T

R

P

L

I

L

L

I

K

G

G

G

D

R

I

S

S

W

G

G

N

N

M

F

V

F

E

E

P

P

T

A

I

I

L

F

T

V

D

D

V

V

T

D

S

R

D

N

M

F

A

R

V

I

A

M

Q

R

E

E

E
|
E

L

V

F

F

G

G

P

P

V

V

V

R

S

P

V

I

M

V

T

V

F

V

E

E

T

N

D

D

E

D

E

Q

A

A

I

V

K

E

I

V

A

A

N

N

D

D

T

T

E

D

F

Y

G

G

L

L

S

S

G

G

A

A

V

V

H

L

T

T

S

N

D

N

G

V

E

N

R

R

G

A

V

F

Q

R

L

I

A

A

K

E

K

A

V

V

H

E

T

S

G

G

M

M

V

F

H

H

V

I

N

N

D

D

I

V

T

T

I

F

N

L

D

E

E

E

P

S

I

H

V

V

A

P

F

F

G

G

E

I

K

K

K

A

S

S

G

G

M

V

G

G

R

R

L
x
E

N

G

G

G

D

E

W

W

S

S

L

F

E

H

E

E

F

T

T

T

T

Y

L

D

K

R

W

W

I

V

S

T

V

V

H

T

Y

L

Q

R

Q

T

R

R

E

R

I

F

P

P

active site: N154 (= N154), K177 (= K177), E252 (= E253), C286 (= C287), E381 (= E381), E459 (≠ L459)
binding nadp nicotinamide-adenine-dinucleotide phosphate: I150 (= I150), T151 (≠ S151), P152 (= P152), W153 (≠ F153), K177 (= K177), S180 (≠ E180), G210 (≠ I211), G214 (= G215), F228 (= F229), G230 (= G231), E231 (≠ S232), T234 (≠ V235), N331 (= N331), R333 (≠ G333), Q334 (≠ A334)

4jz6A Crystal structure of a salicylaldehyde dehydrogenase from pseudomonas putida g7 complexed with salicylaldehyde (see paper)
34% identity, 99% coverage: 8:485/485 of query aligns to 4:484/484 of 4jz6A

query
sites
4jz6A

N

T

K

K

Q

L

Y

F

I

I

A

N

G

N

T

A

W

W

R

I

D

D

G

S

R

S

S

D

N

Q

T

T

L

F

T

E

D

R

K

K

N

H

P

P

Y

V

N

S

G

S

E

E

R

V

I

M

T

T

Q

E

F

S

K

A

M

N

A

A

T

T

T

V

E

T

D

D

V

A

D

I

E

K

A

A

Y

A

K

Q

S

A

A

A

Q

E

D

E

A

A

M

F

D

K

E

T

W

W

N

K

Q

D

I

V

N

G

P

P

Y

S

K

E

Q

R

R

R

D

R

T

L

L

L

E

L

N

K

A

V

V

A

T

D

Y

V

I

M

E

E

E

S

H

K

E

T

E

P

D

K

I

F

T

I

D

E

I

V

I

M

I

A

D

M

E

E

L

V

G

G

G

A

T

S

R

A

L

L

K
x
W

A

A

A

G

F

F

E

N

I

V

G

H

L

A

V

S

K

A

D

N

M

V

I

F

K

R

E

E

A

A

T

S

F

L

P

A

L

T

N

Q

K

I

E

Q

G

G

K

E

I

T

L

I

P

P

S

T

V

D

E

K

N

A

G

E

K

T

E

L

N

S

R

M

L

T

Y

L

R

R

M

Q

P

P

A

V

G

G

V

P

V

I

G

L

V

S

I

I

S

V

P

P

F

W

N
|
N

F
x
G

P

T

F

A

F
x
V

L

L

S

A

I

A

K

R

S

A

V

I

A

A

P

Y

A

P

I

L

G

V

A

C

G

G

N

N

G

T

V

V

L

F

K
|
K

A

G

H

S

E

E

E

F

T

S

P

P

I

A

M

T

G

H

G

A

T

-

L

L

I

I

A

T

K

Q

I

C

F

V

E

Q

E

E

A

A

G

G

V

L

P

P

K

A

G

G

L

V

F

L

N

N

V

Y

V

L

V

N

T

S

D

S

I

P

N

D

-

R

-

S

-

P

E

E

I

I

G

A

D

D

T

A

F

L

V

I

E

S

H

A

P

K

I

E

P

I

R

R

I

R

I

I

S

N

F

F

T

T

G

G

S

S

T

T

K

R

V

V

G

G

S

S

H

I

I

I

G

A

Q

Q

L

K

A

A

V

A

K

Q

H

H

F

L

K

K

K
x
R

P
x
C

I

L

L

L

E
|
E

L

L

G

G

N

K

S

S

A

P

F

L

I

I

I

V

L

L

D

D

A

A

D

D

V

I

D

N

Y

A

A

A

V

V

E

K

A

A

A

V

F

F

S

G

R

S

F

F

T

L

H

F

Q

Q

G

G

Q

Q

I
|
I

C
|
C

M
|
M

S

S

A

T

N

E

R

R

I

L

I

V

V

V

H

D

Q

E

S

K

I

I

Y

A

D

D

E

E

F

F

I

V

T

A

K

R

Y

F

K

V

E

E

K

K

I

T

A

E

S

R

L

L

K

S

T

V

G

G

D

D

P

P

R

C

-

L

D

T

P

G

D

D

T

C

V

I

I

I

G

G

P

P

V

M

V

V

N

S

E

P

G

N

A

S

A

G

E

E

N

R

L

I

K

N

A

G

I

L

I

F

D

K

K

D

G

A

I

I

E

D

Q

K

G

G

A

A

T

K

P

V

I

V

L

C

K

G

G

G

D

M

I

A

S

Q

G

G

N

A

M

V

V

M

E

P

P

A

T

T

I

I

L

L

T

D

D

H

V

V

T

K

S

S

D

D

M

M

A

R

V

I

A

Y

Q

D

E

E

E
|
E

L

T

F

F

G

G

P

P

V

I

V

T

S

V

V

V

M

I

T

R

F

C

E

K

T

G

D

E

E

A

E

E

A

A

I

I

K

R

I

I

A

A

N

N

D

D

T

S

E

V

F

Y

G

G

L

L

S

S

G

S

A

G

V

V

H

F

T

G

S

R

D

D

G

I

E

N

R

R

G

A

V

L

Q

R

L

V

A

G

K

M

K

S

V

I

H

E

T

Y

G

G

M

C

V

V

H

H

V

I

N

N

D

G

I

S

T

T

I

V

N

Q

D

N

E

E

P

A

I

Q

V

A

A

P

F
x
Y

G

G

E

T

K

K

K

N

S

T

G

G

M
x
Y

G

G

R

R

L
x
F

N

D

G

G

D

R

W

A

S

V

L

I

E

D

E

E

F

F

T

T

T

E

L

L

K

K

W

W

I

L

S

T

V

I

H

E

Y

P

Q

F

Q

E

R

Q

E

Q

I

Y

P

P

Y

F

active site: N150 (= N154), K173 (= K177), E251 (= E253), C285 (= C287), E380 (= E381), F458 (≠ L459)
binding salicylaldehyde: W97 (≠ K101), G151 (≠ F155), V154 (≠ F158), R247 (≠ K249), C248 (≠ P250), I284 (= I286), C285 (= C287), M286 (= M288), Y447 (≠ F448), Y455 (≠ M456)

P25526 Succinate-semialdehyde dehydrogenase [NADP(+)] GabD; SSDH; Glutarate-semialdehyde dehydrogenase; EC 1.2.1.79; EC 1.2.1.- from Escherichia coli (strain K12) (see paper)
32% identity, 97% coverage: 8:476/485 of query aligns to 11:480/482 of P25526

query
sites
P25526

N

Q

K

Q

Q

A

Y

L

I

I

A

N

G

G

T

E

W

W

R

L

D

D

G

A

R

N

S

N

N

G

N

E

T

A

L

I

T

D

D

V

K

T

N

N

P

P

Y

A

N

N

G

G

E

D

R

K

I

L

T

G

Q

S

F

V

K

P

M

K

A

M

T

G

T

A

E

D

D

E

V

T

D

R

E

A

A

A

Y

I

K

D

S

A

A

A

Q

N

D

R

A

A

M

L

D

P

E

A

W

W

N

R

Q

A

I

L

N

T

P

A

Y

K

K

E

Q

R

R

A

D

T

T

I

L

L

E

R

N

N

A

W

V

F

T

N

Y

L

I

M

E

M

E

E

H

H

E

Q

E

D

D

D

I

L

T

A

D

R

I

L

I

M

I

T

D

L

E

E

L

Q

G

G

G

K

T

P

R

L

L

A

K

E

A

A

A

K

F

G

E

E

I

I

G

S

L

Y

V

A

K

A

D

S

M

F

I

I

K

E

E

W

A

F

A

A

T

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F

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L

K

N

R

K

I

E

Y

G

G

K

D

I

T

L

I

P

P

S

G

V

H

E

Q

N

A

G

D

K

K

E

R

N

L

R

I

L

V

Y

I

R

K

M

Q

P

P

A

I

G

G

V

V

V

T

G

A

V

A

I

I

S

T

P

P

F
x
W

N
|
N

F

F

P

P

F

A

L

M

S

I

I

T

K

R

S

K

V

A

A

G

P

P

A

A

I

L

G

A

A

A

G

G

N

C

G

T

V

M

V

V

L

L

K
|
K

A
x
P

H
x
A

E
x
S

E

Q

T

T

P

P

I

-

M

F

G

S

G

A

T

L

L

A

I

L

A

A

K

E

I

L

F

A

E

I

E

R

A

A

G

G

V

V

P

P

K

A

G

G

L

V

F

F

N

N

V

V

V

T

T

G

D

S

I

A

N

G

E

A

I

V

G

G

D

N

T

E

F

L

V

T

E

S

H

N

P

P

I

L

P

V

R

R

I

K

I

L

S

S

F

F

T

T

G
|
G

S
|
S

T

T

K

E

V

I

G

G

S

R

H

Q

I

L

G

M

Q

E

L

Q

A

C

V

A

K

K

H

D

F

I

K

K

P

V

I

S

L

L

E

E

L
|
L

G

G

N

N

S

A

A

P

F

F

I

I

I

V

L

F

D

D

A

A

D

D

V

L

D

D

Y

K

A

A

V

V

E

E

A

G

A

A

A

L

F

A

S

S

R

K

F

F

T

R

H

N

Q

A

G

G

Q

Q

I

T

C

C

M

V

S

C

A

A

N

N

R

R

I

L

I

Y

V

V

H

Q

Q

D

S

G

I

V

Y

Y

D

D

E

R

F

F

I

A

T

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K

K

Y

L

K

Q

E

Q

K

A

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A

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S

K

L

L

K

H

T

I

G

G

D

D

P

G

R

L

D

D

P

N

D

G

T

V

V

T

I

I

G

G

P

P

V

L

V

I

N

D

E

E

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K

A

A

A

V

E

A

N

K

L

V

K

E

A

E

I

H

I

I

D

A

K

D

G

A

I

L

E

E

Q

K

G

G

A

A

T

R

P

V

I

V

L

C

K

G

G

G

-

K

-

A

-

H

D

E

I

R

S

G

G

G

N

N

M

F

V

F

E

Q

P

P

T

T

I

I

L

L

T

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D

D

V

V

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S

A

D

N

M

A

A

K

V

V

A

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Q

K

E

E

E
|
E

L

T

F

F

G

G

P

P

V

L

V

A

S

P

V

L

M

F

T

R

F

F

E

K

T

D

D

E

E

A

E

D

A

V

I

I

K

A

I

Q

A

A

N

N

D

D

T

T

E

E

F

F

G

G

L

L

S

A

G

A

A

Y

V

F

H

Y

T

A

S

R

D

D

G

L

E

S

R

R

G

V

V

F

Q

R

L

V

A

G

K

E

K

A

V

L

H

E

T

Y

G

G

M

I

V

V

H

G

V

I

N

N

D

T

I

G

T

I

I

I

N

S

D

N

E

E

P

-

I

V

V

A

A

P

F

F

G

G

E

I

K

K

K

A

S

S

G

G

M

L

G

G

R

R

L

E

N

G

G

S

D

K

W

Y

S

G

L

I

E

E

E

D

F

Y

T

L

T

E

L

I

K

K

W

Y

I

M

S

C

V

I

WN 156:157 (≠ FN 153:154) binding NADP(+)
KPAS 180:183 (≠ KAHE 177:180) binding NADP(+)
GS 233:234 (= GS 231:232) binding NADP(+)
L256 (= L254) binding NADP(+)
E386 (= E381) binding NADP(+)

3jz4A Crystal structure of e. Coli NADP dependent enzyme (see paper)
32% identity, 97% coverage: 8:476/485 of query aligns to 10:479/481 of 3jz4A

query
sites
3jz4A

N

Q

K

Q

Q

A

Y

L

I

I

A

N

G

G

T

E

W

W

R

L

D

D

G

A

R

N

S

N

N

G

N

E

T

A

L

I

T

D

D

V

K

T

N

N

P

P

Y

A

N

N

G

G

E

D

R

K

I

L

T

G

Q

S

F

V

K

P

M

K

A

M

T

G

T

A

E

D

D

E

V

T

D

R

E

A

A

A

Y

I

K

D

S

A

A

A

Q

N

D

R

A

A

M

L

D

P

E

A

W

W

N

R

Q

A

I

L

N

T

P

A

Y

K

K

E

Q

R

R

A

D

T

T

I

L

L

E

R

N

N

A

W

V

F

T

N

Y

L

I

M

E

M

E

E

H

H

E

Q

E

D

D

D

I

L

T

A

D

R

I

L

I

M

I

T

D

L

E

E

L

Q

G

G

G

K

T

P

R

L

L

A

K

E

A

A

A

K

F

G

E

E

I

I

G

S

L

Y

V

A

K

A

D

S

M

F

I

I

K

E

E

W

A

F

A

A

T

E

F

E

P

G

L

K

N

R

K

I

E

Y

G

G

K

D

I

T

L

I

P

P

S

G

V

H

E

Q

N

A

G

D

K

K

E

R

N

L

R

I

L

V

Y

I

R

K

M

Q

P

P

A

I

G

G

V

V

V

T

G

A

V

A

I

I

S

T

P
|
P

F
x
W

N
|
N

F

F

P

P

F

A

L

M

S

I

I

T

K

R

S

K

V

A

A

G

P

P

A

A

I

L

G

A

A

A

G

G

N

C

G

T

V

M

V

V

L

L

K
|
K

A

P

H
x
A

E
x
S

E

Q

T

T

P

P

I

-

M

F

G

S

G

A

T

L

L

A

I

L

A

A

K

E

I

L

F

A

E

I

E

R

A

A

G

G

V

V

P

P

K

A

G

G

L

V

F

F

N

N

V

V

V

T

T

G

D

S

I
x
A

N

G

E

A

I

V

G
|
G

D

N

T

E

F

L

V

T

E

S

H

N

P

P

I

L

P

V

R

R

I

K

I

L

S

S

F

F

T

T

G
|
G

S
|
S

T

T

K

E

V
x
I

G

G

S

R

H

Q

I

L

G

M

Q

E

L

Q

A

C

V

A

K

K

H

D

F

I

K

K

P

V

I

S

L

L

E
|
E

L

L

G

G

N

N

S

A

A

P

F

F

I

I

I

V

L

F

D

D

A

A

D

D

V

L

D

D

Y

K

A

A

V

V

E

E

A

G

A

A

A

L

F

A

S

S

R

K

F

F

T

R

H

N

Q

A

G

G

Q

Q

I

T

C
|
C

M

V

S

C

A

A

N

N

R

R

I

L

I

Y

V

V

H

Q

Q

D

S

G

I

V

Y

Y

D

D

E

R

F

F

I

A

T

E

K

K

Y

L

K

Q

E

Q

K

A

I

M

A

S

S

K

L

L

K

H

T

I

G

G

D

D

P

G

R

L

D

D

P

N

D

G

T

V

V

T

I

I

G

G

P

P

V

L

V

I

N

D

E

E

G

K

A

A

A

V

E

A

N
x
K

L

V

K

E

A

E

I

H

I

I

D

A

K

D

G

A

I

L

E

E

Q

K

G

G

A

A

T

R

P

V

I

V

L

C

K

G

G

G

-

K

-

A

-

H

D

E

I

R

S

G

G

G

N

N

M

F

V

F

E

Q

P

P

T

T

I

I

L

L

T

V

D

D

V

V

T

P

S

A

D

N

M

A

A

K

V

V

A

S

Q

K

E

E

E
|
E

L

T

F
|
F

G

G

P

P

V

L

V

A

S

P

V

L

M

F

T

R

F

F

E

K

T

D

D

E

E

A

E

D

A

V

I

I

K

A

I

Q

A

A

N

N

D

D

T

T

E

E

F

F

G

G

L

L

S

A

G

A

A

Y

V

F

H

Y

T

A

S

R

D

D

G

L

E

S

R

R

G

V

V

F

Q

R

L

V

A

G

K

E

K

A

V

L

H

E

T

Y

G

G

M

I

V

V

H

G

V

I

N

N

D

T

I

G

T

I

I

I

N

S

D

N

E

E

P

-

I

V

V

A

A

P

F

F

G

G

E

I

K

K

K

A

S

S

G

G

M

L

G

G

R

R

L
x
E

N

G

G

S

D

K

W

Y

S

G

L

I

E

E

E

D

F

Y

T

L

T

E

L

I

K

K

W

Y

I

M

S

C

V

I

active site: N156 (= N154), K179 (= K177), E254 (= E253), C288 (= C287), E385 (= E381), E462 (≠ L459)
binding nadp nicotinamide-adenine-dinucleotide phosphate: P154 (= P152), W155 (≠ F153), K179 (= K177), A181 (≠ H179), S182 (≠ E180), A212 (≠ I211), G216 (= G215), G232 (= G231), S233 (= S232), I236 (≠ V235), C288 (= C287), K338 (≠ N337), E385 (= E381), F387 (= F383)

8of1A Structure of aldh5f1 from moss physcomitrium patens in complex with NAD+ in the contracted conformation
33% identity, 96% coverage: 12:476/485 of query aligns to 32:497/505 of 8of1A

query
sites
8of1A

I

I

A

G

G

D

T

K

W

W

R

V

D

D

G

A

R

E

S

N

N

G

N

H

T

T

L

L

T

P

D

V

K

N

N

N

P

P

Y

A

N

T

G

G

E

E

R

I

I

L

T

T

Q

S

F

V

K

P

M

F

A

M

T

G

T

K

E

R

D

E

V

A

D

E

E

K

A

A

Y

I

K

A

S

A

A

A

Q

S

D

Q

A

A

M

F

D

T

E

S

W

W

N

S

Q

K

I

R

N

T

P

A

Y

N

K

D

Q

R

R

S

D

K

T

I

L

L

E

R

N

Q

A

W

V

F

T

N

Y

L

I

L

E

I

E

K

H

N

E

K

E

D

D

D

I

L

T

G

D

K

I

L

I

I

I

V

D

L

E

E

L

Q

G

G

G

K

T

P

R

L

L

A

K

E

A

A

A

V

F

G

E

E

I

I

G

V

L

Y

V

G

K

A

D

A

M

F

I

V

K

E

E

Y

A

Y

A

A

T

E

F

E

P

A

L

K

N

R

K

V

E

Y

G

G

K

D

I

I

L

I

P

P

S

S

V

P

E

F

N

P

G

E

K

K

E

R

N

M

R

L

L

V

Y

M

R

K

M

Q

P

P

A

V

G

G

V

V

G

A

V

A

I
|
I

S
x
A

P
|
P

F
x
W

N

N

F

F

P

P

F

L

F

A

L

M

S

I

I

T

K

R

S

K

V

V

A

A

P

P

A

A

I

L

G

A

A

A

G

G

N

C

G

T

V

V

L

I

K
|
K

A

P

H

S

E

E

E

L

T

T

P

P

I

L

M

T

G

A

G

-

T

L

L

A

I

A

A

A

K

E

I

L

F

A

E

L

E

Q

A

A

G

G

V

I

P

P

K

P

G

G

L

V

F

V

N

N

V

V

V

M

T

G

D

D

I
x
A

N

K

E

G

I

I

G

G

D

D

T

A

F

M

V

L

E

D

H

S

P

T

I

E

P

V

R

R

I

K

I

I

S

T

F
|
F

T

T

G
|
G

S
|
S

T

T

K

G

V
|
V

G

G

S

K

H
x
M

I

L

G

L

Q

A

L

G

A

A

V

G

K

K

H

T

F

V

K

K

P

V

I

S

L

L

E

E

L
|
L

G

G

N

N

S

A

A

P

F

C

I

I

I

V

L

F

D

D

A

A

D

N

V

L

D

D

Y

V

A

A

V

V

E

K

A

G

A

V

A

L

F

A

S

G

R

K

F

Y

T

R

H

N

Q

S

G

G

Q

Q

I

T

C
|
C

M

V

S

C

A

I

N

N

R

K

I

I

I

F

V

V

H

Q

Q

D

S

G

I

I

Y

Y

D

D

E

K

F

F

I

A

T

E

K

A

Y

F

K

A

E

K

K

A

I

V

A

S

S

G

L

L

K

R

T

A

G

G

D

N

P

G

R

L

D

E

P

P

D

G

T

I

V

T

I

Q

G

G

P

P

V

L

V

I

N

N

E

E

G

T

A

A

A

L

E

E

N
x
K

L

V

K

E

A

R

I

H

I

V

D

Q

K

D

G

A

I

V

E

S

Q

K

G

G

A

A

T

K

P

V

I

L

L

V

K

G

G

G

-

K

-

R

-

H

D

S

I

L

S

G

G

R

N

T

M

F

V

Y

E

E

P

P

T

T

I

I

L

L

T

G

D

N

V

A

T

S

S

D

D

E

M

M

A

L

V

I

A

F

Q

R

E

E

E
|
E

L

V

F
|
F

G

G

P

P

V

V

V

A

S

P

V

L

M

V

T

R

F

F

E

N

T

T

D

D

E

E

A

A

I

I

K

K

I

L

A

A

N

N

D

N

T

S

E

E

F

F

G

G

L

L

S

A

G

A

A

Y

V

A

H

F

T

T

S

E

D

N

G

I

E

T

R

R

G

G

V

W

Q

R

L

V

A

A

K

E

K

S

V

L

H

E

T

F

G

G

M

M

V

V

H

G

V

L

N

N

D

E

I

G

T

L

I

I

N

S

D

T

E

E

P

-

I

V

V

A

A

P

F

F

G

G

E

M

K

K

K

Q

S

S

G

G

M

L

G

G

R

R

L

E

N

G

G

S

D

K

W

Y

S

G

L

L

E

D

E

E

F

Y

T

L

T

E

L

M

K

K

W

Y

I

V

S

C

V

L

binding nicotinamide-adenine-dinucleotide: I170 (= I150), A171 (≠ S151), P172 (= P152), W173 (≠ F153), K197 (= K177), A230 (≠ I211), F248 (= F229), G250 (= G231), S251 (= S232), V254 (= V235), M257 (≠ H238), L273 (= L254), C306 (= C287), K356 (≠ N337), E403 (= E381), F405 (= F383)

3ty7B Crystal structure of aldehyde dehydrogenase family protein from staphylococcus aureus
36% identity, 88% coverage: 9:437/485 of query aligns to 7:436/454 of 3ty7B

query
sites
3ty7B

K

K

Q

Q

Y

Y

I

I

A

N

G

G

T

E

W

W

R

V

D

E

G

S

R

N

S

S

N

N

N

E

T

T

L

I

T

E

D

V

K
x
I

N

N

P

P

Y

A

N

T

G

E

E

E

R

V

I

I

T

G

Q

K

F

V

K

A

M

K

A

G

T

N

T

K

E

A

D

D

V

V

D

D

E

K

A

A

Y

V

K

E

S

A

A

A

Q

D

D

D

A

V

M

Y

D

L

E

E

W

F

N

R

Q

H

I

T

N

S

P

V

Y

K

K

E

Q

R

R

Q

D

A

T

L

L

L

E

D

N

K

A

I

V

V

T

K

Y

E

I

Y

E

E

E

N

H

R

E

K

E

D

D

D

I

I

T

V

D

Q

I

A

I

I

I

T

D

D

E

E

L

L

G

G

G

A

-

P

-

L

-

S

T

L

R

S

L

E

K

R

A

V

A

H

F

Y

E

Q

I

M

G

G

L

L

V

N

K

H

D

F

M

V

I

A

K

A

E

R

A

D

A

A

T

L

F

D

P

N

L

Y

N

E

K

F

E

E

G

-

K

-

I

-

L

-

P

-

S

-

V

-

E

E

N

R

G

R

K

G

E

D

N

D

R

L

L

V

Y

V

R

K

M

E

P

A

A

I

G

G

V

V

V

S

G

G

V

L

I

I

S

T

P

P

F

W

N
|
N

F

F

P

P

F

T

F

N

L

Q

S

T

I

S

K

L

S

K

V

L

A

A

P

A

A

A

I

F

G

A

A

A

G

G

N

S

G

P

V

V

L

L

K
|
K

A

P

H

S

E

E

E
|
E

T

T

P

P

I

-

M

F

G

A

T

V

L

I

I

L

A

A

K

E

I

I

F

F

E

D

E

K

A

V

G

G

V

V

P

P

K

K

G

G

L

V

F

F

N

N

V

L

V

V

V

N

T

G

D

D

I

G

N

A

E

G

I

V

G

G

D

N

T

P

F

L

V

S

E

E

H

H

P

P

I

K

P

V

R

R

I

M

S

S

F

F

T

T

G

G

S

S

T

G

K

P

V

T

G

G

S

S

H

K

I

I

G

M

Q

E

L

K

A

A

V

A

K

K

H

D

F

F

K

K

P

V

I

S

L

L

E
|
E

L

L

G

G

N

K

S

S

A

P

F

Y

I

I

I

V

L

L

D

D

A

V

D

D

V

I

D

K

Y

E

A

A

V

A

E

K

A

A

A

T

F

G

S

K

R

V

F

V

T

N

H

N

Q

T

G

G

Q

Q

I

V

C
|
C

M

T

S

A

A

G

N

T

R

R

I

V

I

L

V

V

H

P

Q

N

S

K

I

I

Y

K

D

D

E

A

F

F

I

L

T

A

K

E

Y

L

K

K

E

E

K

Q

I

F

A

S

S

Q

L

V

K

R

T

V

G

G

D

N

P

P

R

R

D

E

P

D

D

G

T

T

V

Q

I

V

G

G

P

P

V

I

N

S

E

K

G

K

A

Q

A

F

E

D

N

Q

L

V

K

Q

A

N

I

Y

I

I

D

N

K

K

G

G

I

I

E

E

Q

E

G

G

A

A

T

E

P

L

I

F

L

Y

-

G

-

P

-

G

-

K

-

P

K

E

G

G

D

L

I

E

S

K

G

G

N

Y

M

F

V

A

E

R

P

P

T

T

I

I

L

F

T

I

D

N

V

V

T

D

S

N

D

Q

M

M

A

T

V

I

A

A

Q

Q

E

E

E
|
E

L

I

F

F

G

G

P

P

V

V

V

M

S

S

V

V

M

I

T

T

F

Y

E

N

T

D

D

L

E

D

E

E

A

A

I

I

K

Q

I

I

A

A

N

N

D

D

T

T

E

K

F

Y

G

G

L

L

S

A

G

G

A

Y

V

V

H

I

T

G

S

K

D

D

G

K

E

E

R

T

G

L

V

H

Q

K

L

V

A

A

K

R

K

S

V

I

H

E

T

A

G

G

M

T

V

V

H

E

V

I

N

N

D

E

active site: N148 (= N154), K171 (= K177), E246 (= E253), C280 (= C287), E380 (= E381)
binding magnesium ion: I26 (≠ K28), E175 (= E181)

Q59931 NADP-dependent glyceraldehyde-3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase [NADP(+)]; Non-phosphorylating glyceraldehyde 3-phosphate dehydrogenase; Triosephosphate dehydrogenase; EC 1.2.1.9 from Streptococcus mutans serotype c (strain ATCC 700610 / UA159) (see 3 papers)
33% identity, 96% coverage: 9:474/485 of query aligns to 6:470/475 of Q59931

query
sites
Q59931

K

K

Q

N

Y

Y

I

V

A

N

G

G

T

E

W

W

R

K

D

-

G

-

R

L

S

S

N

E

N

N

T

E

L

I

T

K

D

I

K

Y

N

E

P

P

Y

A

N

S

G

G

E

A

R

E

I

L

T

G

Q

S

F

V

K

P

M

A

A

M

T

S

T

T

E

E

D

E

V

V

D

D

E

Y

A

V

Y

Y

K

A

S

S

A

A

Q

K

D

K

A

A

M

Q

D

P

E

A

W

W

N

R

Q

S

I

L

N

S

P

Y

Y

I

K

E

Q

R

R

A

D

A

T

Y

L

L

E

H

N

K

A

V

V

A

T

D

Y

I

I

L

E

M

E

R

H

D

E

K

E

E

D

K

I

I

T

G

D

A

I

V

I

L

I

S

D

K

E

E

L

V

G

A

G

K

T

G

R

Y

L

K

K

S

A

A

A

V

F

S

E

E

I

V

G

V

L
x
R

V

T

K

A

D

E

M

I

I

I

K

N

E

Y

A

A

T

E

F

E

P

G

L

L

N

R

K

M

E

E

G

G

K

E

I

V

L

L

P

E

-

G

S

S

V

F

E

E

N

A

G

A

K

S

E

K

N

K

R

K

L

I

-

A

-

V

Y

R

R

R

M

E

P

P

A

V

G

G

V

L

V

V

G

L

V

A

I

I

S
|
S

P

P

F

F

N

N

F

Y

P

P

F

V

F

N

L

L

S

A

I

G

K

S

S

K

V

I

A

A

P

P

A

A

I

L

G

I

A

A

G

G

N

N

G

V

V

I

V

A

L

F

K
|
K

A

P

H

P

E
x
T

E

Q

T

G

P

S

I

I

M

-

G

S

G

G

T

L

L

L

I

L

A

A

K

E

I

A

F

F

E

A

E

E

A

A

G

G

V

L

P

P

K

A

G

G

L

V

F

F

N

N

V

T

V

I

V

T

T

G

D

R

I

G

N

S

E

E

I

I

G

G

D
|
D

T

Y

F

I

V

V

E

E

H

H

P

Q

I

A

P

V

R

N

I

F

I

I

S

N

F

F

T

T

G
|
G

S
|
S

T
|
T

K
x
G

V
x
I

G
|
G

S
x
E

H
x
R

I
|
I

G
|
G