SitesBLAST
Comparing WP_010631287.1 NCBI__GCF_000246965.1:WP_010631287.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4om8A Crystal structure of 5-formly-3-hydroxy-2-methylpyridine 4-carboxylic acid (fhmpc) 5-dehydrogenase, an NAD+ dependent dismutase. (see paper)
34% identity, 100% coverage: 1:312/312 of query aligns to 1:309/309 of 4om8A
- active site: S116 (= S119), H137 (= H140), E149 (= E152), N188 (= N191)
- binding nicotinamide-adenine-dinucleotide: G11 (= G11), T12 (≠ V12), M13 (= M13), Y31 (≠ N31), D32 (= D32), V33 (≠ I33), N86 (≠ A89), V87 (≠ I90), P88 (= P91), E89 (= E92), K94 (= K97), D114 (≠ N117), H137 (= H140)
Q988C8 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylate 5-dehydrogenase; FHMPC dehydrogenase; EC 1.2.1.100 from Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099) (Mesorhizobium loti (strain MAFF 303099)) (see 2 papers)
34% identity, 100% coverage: 1:312/312 of query aligns to 1:309/309 of Q988C8
- TM 12:13 (≠ VM 12:13) binding NAD(+)
- D32 (= D32) binding NAD(+)
- VPE 87:89 (≠ IPE 90:92) binding NAD(+)
- K94 (= K97) binding NAD(+)
- H137 (= H140) mutation to L: Loss of activity.
- E149 (= E152) mutation to Q: Shows a different pH optimum depending on the cosubstrate.
6aa8E Crystal structure of (s)-3-hydroxybutyryl-coenzymea dehydrogenase from clostridium acetobutylicum complexed with NAD+ (see paper)
39% identity, 91% coverage: 3:286/312 of query aligns to 1:280/281 of 6aa8E
- active site: S116 (= S119), H137 (= H140), E149 (= E152), N187 (= N191)
- binding nicotinamide-adenine-dinucleotide: I6 (= I8), G9 (= G11), T10 (≠ V12), M11 (= M13), R29 (≠ N31), D30 (= D32), I31 (= I33), A86 (= A89), A87 (≠ I90), E89 (= E92), K94 (= K97), N114 (= N117), S116 (= S119)
4kugA Crystal structure of 3-hydroxybutylryl-coa dehydrogenase with NAD from clostridium butyricum
38% identity, 91% coverage: 2:286/312 of query aligns to 1:281/282 of 4kugA
- active site: S117 (= S119), H138 (= H140), E150 (= E152), N188 (= N191)
- binding nicotinamide-adenine-dinucleotide: G10 (= G11), T11 (≠ V12), M12 (= M13), R30 (≠ N31), D31 (= D32), A87 (= A89), A88 (≠ I90), E90 (= E92), N115 (= N117), S117 (= S119), H138 (= H140)
4kuhA Crystal structure of 3-hydroxybutylryl-coa dehydrogenase with acetoacetyl-coa from clostridium butyricum
38% identity, 91% coverage: 2:285/312 of query aligns to 1:280/280 of 4kuhA
4pzeA Crystal structure of (s)-3-hydroxybutyryl-coa dehydrogenase paah1 in complex with acetoacetyl-coa (see paper)
34% identity, 91% coverage: 2:286/312 of query aligns to 2:282/283 of 4pzeA
4pzdA Crystal structure of (s)-3-hydroxybutyryl-coa dehydrogenase paah1 in complex with NAD+ (see paper)
34% identity, 91% coverage: 2:286/312 of query aligns to 2:282/283 of 4pzdA
- active site: S118 (= S119), H139 (= H140), E151 (= E152), N189 (= N191)
- binding nicotinamide-adenine-dinucleotide: G11 (= G11), T12 (≠ V12), M13 (= M13), D32 (= D32), A88 (= A89), A89 (≠ I90), T90 (≠ P91), E91 (= E92), I99 (≠ L100), N116 (= N117), S118 (= S119), N142 (= N143)
P9WNP7 3-hydroxybutyryl-CoA dehydrogenase; Beta-hydroxybutyryl-CoA dehydrogenase; BHBD; EC 1.1.1.157 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
34% identity, 91% coverage: 2:285/312 of query aligns to 5:286/286 of P9WNP7
- S122 (= S119) mutation to A: Loss of fatty acyl dehydrogenase activity.
P00348 Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial; HCDH; L-3-hydroxyacyl CoA dehydrogenase; Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; Short-chain 3-hydroxyacyl-CoA dehydrogenase; EC 1.1.1.35 from Sus scrofa (Pig) (see paper)
35% identity, 91% coverage: 1:285/312 of query aligns to 26:313/314 of P00348
- GGGLMG 34:39 (≠ GSGVMG 9:14) binding NAD(+)
- D57 (= D32) binding NAD(+)
- E122 (= E92) binding NAD(+)
- K127 (= K97) binding NAD(+)
- S149 (= S119) binding NAD(+)
Q16836 Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial; HCDH; Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; Short-chain 3-hydroxyacyl-CoA dehydrogenase; EC 1.1.1.35 from Homo sapiens (Human) (see 7 papers)
34% identity, 91% coverage: 1:285/312 of query aligns to 26:313/314 of Q16836
- GGGLMG 34:39 (≠ GSGVMG 9:14) binding NAD(+)
- A40 (≠ S15) to T: in HADH deficiency; dbSNP:rs137853101
- D57 (= D32) binding NAD(+); to E: in HADH deficiency; dbSNP:rs137853102; to G: found in a patient with Reye-like syndrome; does not affect 3-hydroxyacyl-CoA dehydrogenase activity; increases KM value for NADH; does not affect dimerization
- S73 (≠ N48) binding CoA
- K80 (vs. gap) binding CoA
- L86 (= L58) to P: in dbSNP:rs4956145
- E122 (= E92) binding NAD(+)
- K127 (= K97) binding NAD(+); modified: N6-(2-hydroxyisobutyryl)lysine
- S149 (= S119) binding CoA; binding NAD(+)
- Q152 (≠ P122) to H: in dbSNP:rs1051519
- N173 (= N143) binding NAD(+)
- Y226 (≠ L197) to H: found in a patient with Reye-like syndrome; loss of 3-hydroxyacyl-CoA dehydrogenase activity. Does not affect dimerization; dbSNP:rs146036912
- P258 (= P232) to L: in HHF4; loss of 3-hydroxyacyl-CoA dehydrogenase activity; dbSNP:rs137853103
- K305 (= K277) binding NAD(+)
1f17A L-3-hydroxyacyl-coa dehydrogenase complexed with nadh (see paper)
35% identity, 91% coverage: 1:285/312 of query aligns to 3:290/293 of 1f17A
- active site: S126 (= S119), H147 (= H140), E159 (= E152), N197 (= N191)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G13 (= G11), L14 (≠ V12), M15 (= M13), D34 (= D32), Q35 (≠ I33), A96 (= A89), I97 (= I90), E99 (= E92), K104 (= K97), N124 (= N117), S126 (= S119), H147 (= H140)
1f12A L-3-hydroxyacyl-coa dehydrogenase complexed with 3-hydroxybutyryl-coa (see paper)
35% identity, 91% coverage: 1:285/312 of query aligns to 3:290/293 of 1f12A
- active site: S126 (= S119), H147 (= H140), E159 (= E152), N197 (= N191)
- binding 3-hydroxybutanoyl-coenzyme a: K57 (≠ G56), S126 (= S119), H147 (= H140), F149 (= F142), N150 (= N143), M155 (≠ V148), N197 (= N191), L200 (≠ Q194), P232 (≠ R226), M233 (≠ V230), L238 (≠ I235)
1f0yA L-3-hydroxyacyl-coa dehydrogenase complexed with acetoacetyl-coa and NAD+ (see paper)
35% identity, 91% coverage: 1:285/312 of query aligns to 3:290/291 of 1f0yA
- active site: S126 (= S119), H147 (= H140), E159 (= E152), N197 (= N191)
- binding acetoacetyl-coenzyme a: S50 (≠ N48), K57 (≠ G56), S126 (= S119), H147 (= H140), F149 (= F142), N150 (= N143), P151 (= P144), P153 (≠ N146), V154 (≠ L147), N197 (= N191), P232 (≠ R226), M233 (≠ V230), L238 (≠ I235)
- binding nicotinamide-adenine-dinucleotide: G13 (= G11), L14 (≠ V12), M15 (= M13), D34 (= D32), Q35 (≠ I33), A96 (= A89), I97 (= I90), E99 (= E92), K104 (= K97), N124 (= N117), S126 (= S119), H147 (= H140), N150 (= N143), V242 (≠ G239), T246 (= T243), K282 (= K277)
D7URM0 L-carnitine dehydrogenase; CDH; L-CDH; EC 1.1.1.108 from Pseudomonas sp. (see paper)
32% identity, 94% coverage: 3:295/312 of query aligns to 8:306/321 of D7URM0
Sites not aligning to the query:
- 317:321 mutation Missing: Marked decrease in catalytic activity.
3f3sA The crystal structure of human lambda-crystallin, cryl1
30% identity, 99% coverage: 1:308/312 of query aligns to 1:309/312 of 3f3sA