SitesBLAST
Comparing WP_010631563.1 NCBI__GCF_000246965.1:WP_010631563.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4nbuB Crystal structure of fabg from bacillus sp (see paper)
35% identity, 96% coverage: 8:263/267 of query aligns to 5:241/244 of 4nbuB
- active site: G18 (= G21), N111 (= N120), S139 (= S148), Q149 (= Q158), Y152 (= Y161), K156 (= K165)
- binding acetoacetyl-coenzyme a: D93 (≠ P93), K98 (≠ E107), S139 (= S148), N146 (≠ S155), V147 (≠ N156), Q149 (= Q158), Y152 (= Y161), F184 (≠ I193), M189 (≠ L199), K200 (≠ Y223)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G17), N17 (≠ S20), G18 (= G21), I19 (= I22), D38 (= D41), F39 (≠ I42), V59 (≠ M59), D60 (= D60), V61 (= V61), N87 (≠ D87), A88 (= A88), G89 (= G89), I90 (= I90), T137 (≠ I146), S139 (= S148), Y152 (= Y161), K156 (= K165), P182 (= P191), F184 (≠ I193), T185 (≠ N194), T187 (= T197), M189 (≠ L199)
3ak4A Crystal structure of nadh-dependent quinuclidinone reductase from agrobacterium tumefaciens
36% identity, 96% coverage: 8:263/267 of query aligns to 5:254/258 of 3ak4A
- active site: G18 (= G21), S141 (= S148), L151 (≠ Q158), Y154 (= Y161), K158 (= K165), E199 (= E206)
- binding nicotinamide-adenine-dinucleotide: K17 (≠ S20), G18 (= G21), I19 (= I22), D38 (= D41), L39 (≠ I42), V60 (≠ M59), D61 (= D60), V62 (= V61), N88 (≠ D87), A89 (= A88), G90 (= G89), T139 (≠ I146), S141 (= S148), Y154 (= Y161), K158 (= K165), G185 (= G192), V187 (≠ N194), T189 (≠ K196), M191 (≠ G198)
3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
36% identity, 95% coverage: 11:263/267 of query aligns to 5:243/246 of 3osuA
5itvA Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
33% identity, 97% coverage: 6:263/267 of query aligns to 3:251/255 of 5itvA
- active site: G18 (= G21), S141 (= S148), Y154 (= Y161), K158 (= K165)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G17), S17 (= S20), G18 (= G21), I19 (= I22), D38 (= D41), I39 (= I42), T61 (≠ M59), I63 (≠ V61), N89 (≠ D87), G91 (= G89), T139 (≠ I146), S141 (= S148), Y154 (= Y161), K158 (= K165), P184 (= P191), G185 (= G192), I186 (= I193), I187 (vs. gap)
3sj7A Structure of beta-ketoacetyl-coa reductase (fabg) from staphylococcus aureus complex with NADPH (see paper)
35% identity, 95% coverage: 11:263/267 of query aligns to 2:236/239 of 3sj7A
- active site: G12 (= G21), S138 (= S148), Q148 (= Q158), Y151 (= Y161), K155 (= K165)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G17), S10 (≠ A19), R11 (≠ S20), I13 (= I22), N31 (vs. gap), Y32 (vs. gap), A33 (vs. gap), G34 (vs. gap), S35 (vs. gap), A58 (≠ M59), N59 (≠ D60), V60 (= V61), N86 (≠ D87), A87 (= A88), T109 (≠ V119), S138 (= S148), Y151 (= Y161), K155 (= K165), P181 (= P191), G182 (= G192)
P0AEK2 3-oxoacyl-[acyl-carrier-protein] reductase FabG; 3-ketoacyl-acyl carrier protein reductase; Beta-Ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; EC 1.1.1.100 from Escherichia coli (strain K12) (see 2 papers)
36% identity, 97% coverage: 6:263/267 of query aligns to 1:240/244 of P0AEK2
- GASR 12:15 (≠ GGAS 17:20) binding NADP(+)
- T37 (≠ Q49) binding NADP(+)
- NV 59:60 (≠ DV 60:61) binding NADP(+)
- N86 (≠ D87) binding NADP(+)
- Y151 (= Y161) mutation to F: Defect in the affinity for NADPH.
- YAAAK 151:155 (≠ YSATK 161:165) binding NADP(+)
- A154 (≠ T164) mutation to T: Decreases in the thermolability of the reductase; when associated with K-233.
- K155 (= K165) mutation to A: Defect in the affinity for NADPH.
- I184 (≠ N194) binding NADP(+)
- E233 (≠ T256) mutation to K: Decreases in the thermolability of the reductase; when associated with T-154.
1q7bA The structure of betaketoacyl-[acp] reductase from e. Coli in complex with NADP+ (see paper)
36% identity, 96% coverage: 8:263/267 of query aligns to 2:239/243 of 1q7bA
- active site: G15 (= G21), E101 (≠ D112), S137 (= S148), Q147 (= Q158), Y150 (= Y161), K154 (= K165)
- binding calcium ion: E232 (≠ T256), T233 (= T257)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G17), S13 (≠ A19), R14 (≠ S20), T36 (≠ Q49), N58 (≠ D60), V59 (= V61), N85 (≠ D87), A86 (= A88), G87 (= G89), I88 (= I90), S137 (= S148), Y150 (= Y161), K154 (= K165), P180 (= P191), G181 (= G192), I183 (≠ N194)
4i08A Crystal structure of beta-ketoacyl-acyl carrier protein reductase (fabg) from vibrio cholerae in complex with NADPH (see paper)
35% identity, 97% coverage: 5:263/267 of query aligns to 3:239/243 of 4i08A
- active site: G19 (= G21), N113 (= N120), S141 (= S148), Q151 (= Q158), Y154 (= Y161), K158 (= K165)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G15 (= G17), S17 (≠ A19), R18 (≠ S20), I20 (= I22), T40 (≠ I42), N62 (≠ D60), V63 (= V61), N89 (≠ D87), A90 (= A88), G140 (≠ S147), S141 (= S148), Y154 (= Y161), K158 (= K165), P184 (= P191), G185 (= G192), T189 (= T201)
1q7cA The structure of betaketoacyl-[acp] reductase y151f mutant in complex with NADPH fragment (see paper)
36% identity, 96% coverage: 8:263/267 of query aligns to 2:239/243 of 1q7cA
- active site: G15 (= G21), S137 (= S148), Q147 (= Q158), F150 (≠ Y161), K154 (= K165)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (= G17), S13 (≠ A19), R14 (≠ S20), A35 (≠ D41), T36 (≠ Q49), L57 (≠ M59), N58 (≠ D60), V59 (= V61), G87 (= G89), I88 (= I90)
3op4A Crystal structure of putative 3-ketoacyl-(acyl-carrier-protein) reductase from vibrio cholerae o1 biovar eltor str. N16961 in complex with NADP+ (see paper)
35% identity, 97% coverage: 5:263/267 of query aligns to 3:243/247 of 3op4A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G17), S17 (≠ A19), R18 (≠ S20), I20 (= I22), T40 (≠ I42), N62 (≠ D60), V63 (= V61), N89 (≠ D87), A90 (= A88), I92 (= I90), V139 (≠ I146), S141 (= S148), Y154 (= Y161), K158 (= K165), P184 (= P191), G185 (= G192), I187 (≠ N194), T189 (= T197), M191 (≠ L199)
6t77A Crystal structure of klebsiella pneumoniae fabg(NADPH-dependent) NADP- complex at 1.75 a resolution (see paper)
36% identity, 97% coverage: 6:263/267 of query aligns to 1:240/244 of 6t77A
- active site: G16 (= G21), S138 (= S148), Y151 (= Y161)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G17), S14 (≠ A19), R15 (≠ S20), T37 (≠ I42), L58 (≠ M59), N59 (≠ D60), V60 (= V61), A87 (= A88), G88 (= G89), I89 (= I90)
4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
35% identity, 97% coverage: 6:263/267 of query aligns to 1:244/247 of 4jroC
- active site: G16 (= G21), S142 (= S148), Q152 (= Q158), Y155 (= Y161), K159 (= K165)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G17), S14 (≠ A19), R15 (≠ S20), G16 (= G21), I17 (= I22), N35 (vs. gap), Y36 (vs. gap), N37 (vs. gap), G38 (vs. gap), S39 (vs. gap), N63 (≠ D60), V64 (= V61), N90 (≠ D87), A91 (= A88), I93 (= I90), I113 (≠ V119), S142 (= S148), Y155 (= Y161), K159 (= K165), P185 (= P191), I188 (≠ L199), T190 (= T201)
P73574 3-oxoacyl-[acyl-carrier-protein] reductase; 3-ketoacyl-acyl carrier protein reductase; EC 1.1.1.100 from Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa) (see paper)
37% identity, 96% coverage: 7:263/267 of query aligns to 3:243/247 of P73574
- A14 (≠ G18) mutation to G: 4.2-fold increase in activity on acetoacetyl-CoA.
- P151 (≠ N156) mutation to F: 2.7-fold increase in activity on acetoacetyl-CoA.; mutation to V: 5.7-fold increase in activity on acetoacetyl-CoA.
- K160 (= K165) mutation to A: Almost no activity on acetoacetyl-CoA.
- F188 (≠ I193) mutation to Y: 3.3-fold increase in activity on acetoacetyl-CoA.
- N198 (≠ S215) mutation to R: 3.5-fold increase in activity on acetoacetyl-CoA.
P0A2C9 3-oxoacyl-[acyl-carrier-protein] reductase FabG; 3-ketoacyl-acyl carrier protein reductase; Beta-Ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; EC 1.1.1.100 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
34% identity, 97% coverage: 6:263/267 of query aligns to 1:240/244 of P0A2C9
- M125 (≠ F135) mutation to I: Loss of the temperature-sensitive phenotype; when associated with T-223.
- A223 (≠ L246) mutation to T: Loss of the temperature-sensitive phenotype; when associated with I-125.
- S224 (= S247) mutation to F: Distorts the local conformation and prevent stacking around Phe-221. The S224F mutation would additionally disrupt the hydrogen bond formed between Ser-224 and Glu-226.
6pejA Structure of sorbitol dehydrogenase from sinorhizobium meliloti 1021 bound to sorbitol
32% identity, 96% coverage: 8:263/267 of query aligns to 4:253/257 of 6pejA
Q9KJF1 (2S)-[(R)-hydroxy(phenyl)methyl]succinyl-CoA dehydrogenase subunit BbsD; (S,R)-2-(alpha-hydroxybenzyl)succinyl-CoA dehydrogenase subunit BbsD; EC 1.1.1.429 from Thauera aromatica (see 2 papers)
35% identity, 97% coverage: 6:265/267 of query aligns to 1:244/248 of Q9KJF1
- M1 (≠ L6) modified: Initiator methionine, Removed
- S15 (= S20) binding NAD(+)
- D36 (= D41) binding NAD(+)
- D62 (= D60) binding NAD(+)
- I63 (≠ V61) binding NAD(+)
- N89 (≠ D87) binding NAD(+)
- Y153 (= Y161) binding NAD(+)
- K157 (= K165) binding NAD(+)
7tzpG Crystal structure of putataive short-chain dehydrogenase/reductase (fabg) from klebsiella pneumoniae subsp. Pneumoniae ntuh-k2044 in complex with nadh (see paper)
34% identity, 97% coverage: 6:263/267 of query aligns to 4:244/247 of 7tzpG