SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_010632015.1 NCBI__GCF_000246965.1:WP_010632015.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 1 hits to proteins with known functional sites (download)

7bkbC Formate dehydrogenase - heterodisulfide reductase - formylmethanofuran dehydrogenase complex from methanospirillum hungatei (hexameric, composite structure) (see paper)
31% identity, 13% coverage: 401:496/739 of query aligns to 72:166/189 of 7bkbC

query
sites
7bkbC

V

L

G

G

L

L

I

E

G

N

D

E

V

A

I

L

T

T

E

D

D

P

E

D

I

L

W

W

A

L

C
|
C

T
|
T

C
|
C

R
x
Y

N

S

C
|
C

E

T

D

D

Q

R

C
|
C

P
|
P

V

R

D

D

N

I

E

A

H

P

V
x
T

D

D

K

V

L

I

L

M

D

A

L

M

R

R

R

N

Y

-

L

L

V

A

L

F

M

K

E

R

G

D

K

I

V

V

P

P

Q

K

E

N

A

F

Q

L

R

Q

A

T

M

V

Q

Q

N

L

I

I

E

Y

R

N

Q

S

G

G

N

H

P

-

W

-

G

G

M

V

S

P

R

N

N

N

D

D

R

V

T

N

K

R

W

A

M

A

D

R

G

T

L

K

G

L

G

G

L

L

K

P

V

A

-

D

-

P

P

P

T

T

V

T

K

H

E

S

N

Y

P

P

D

E

F

F

binding iron/sulfur cluster: C87 (= C416), T88 (= T417), T89 (= T418), C90 (= C419), Y91 (≠ R420), C93 (= C422), C97 (= C426), P98 (= P427), T104 (≠ V433)

Sites not aligning to the query:

binding iron/sulfur cluster: 43, 45, 46, 47, 49, 53, 54, 61, 63

Query Sequence

>WP_010632015.1 NCBI__GCF_000246965.1:WP_010632015.1
MWQLLNFLLFLLVTAAFLLMTVTVIFRRYHNIKLGKPVQFAKETRKRINAVLLKVFGQKK
LLKDRKSGLMHVVMFYGFIILQFGALDIIFKGLTGHHLPYPAYPFFQLSQELTALLVLIA
AGYAFYRRYIEKLKRLKRGWKPSLVIIFLTSLMFAVLFTLGFDRLRTGEPGSLLAPASSL
IGALFPGMTATAATGLYYFAWWAHLLILLSFALYIPQSKHFHLITAPVNVFLGRTGPPGK
LQPVDLEDEQAESFGAGRIEDFTQKQLLDLYACVECGRCTSMCPAAATGKVLSPMHLITK
MRDHLTEKGAAVTSRSPWLPPFKEENGIHAMQMTSCPEGTWIDSRVTGETAATGFTPPDQ
PLSAGATVLPGSARPITDIAPTIAWHQRYWQKDEEKKMDTVGLIGDVITEDEIWACTTCR
NCEDQCPVDNEHVDKLLDLRRYLVLMEGKVPQEAQRAMQNIERQGNPWGMSRNDRTKWMD
GLGGLKVPTVKENPDFEYLFFVGSMGSYDNRTIKVTRAFVRLLNKAGVNFAILGNEEKNS
GDTPRRMGNEFLFQELCRENIATFEKYHVRKIVTACPHTFNALKNEYPDFGLKAEVVHHT
QLLDQLIREGKLVPEYPINARITYHDSCYLGRYNGVYDPPRSVLKAIQGVALVEMKRSRE
KAMCCGAGGGRMWMEEKAGKRVNIARTEQALEVHPGIISSACPYCLTMLSDGTKMKGVDD
HVLTKDVAEILAESVFGPE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory