SitesBLAST
Comparing WP_010876115.1 NCBI__GCF_000008645.1:WP_010876115.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P07003 Pyruvate dehydrogenase [ubiquinone]; Pyruvate oxidase; POX; Pyruvate:ubiquinone-8 oxidoreductase; EC 1.2.5.1 from Escherichia coli (strain K12) (see 4 papers)
32% identity, 89% coverage: 52:545/553 of query aligns to 3:525/572 of P07003
- E50 (= E99) binding
- 183:334 (vs. 232:382, 30% identical) FAD-binding domain
- S210 (≠ F259) binding
- LR 234:235 (≠ FR 283:284) binding
- TGLI 251:254 (≠ HGSL 300:303) binding
- TQFPY 274:278 (≠ SSFSD 323:327) binding
- D292 (= D340) binding
- S297 (≠ M345) binding
- DI 311:312 (≠ RS 359:360) binding
- T382 (≠ E430) binding
- FN 403:404 (≠ VT 451:452) binding
- GSM 406:408 (= GSM 454:456) binding
- D433 (vs. gap) binding
- DGG 433:435 (vs. gap) binding
- N460 (= N480) binding
- 460:466 (vs. 480:486, 29% identical) binding
- V462 (≠ N482) binding
- F465 (≠ M485) Moves into active site upon enzyme activation, plays a role in electron transfer
Sites not aligning to the query:
- 1:182 Pyr domain
- 335:530 PP-binding domain
- 531:572 Membrane-binding domain
- 533 A→T: In poxB11; poor activity in vivo, no longer activated by lipids.
- 549:550 In vitro cleavage to yield alpha-peptide
- 549:572 mutation Missing: In poxB6. Inactive in vivo, does not complement inactive mutants. Active in vitro, no longer activated by nor binds to, detergents.
- 553 A→V: In poxB14; poor activity in vivo, no longer activated by lipids.
- 560 D→P: In poxB15; normal activity.
- 564 E→P: In poxB16; loss of activity, weakly activated by cleavage.
- 564:572 mutation Missing: In poxB7 Inactive in vivo, reduced activity in vitro.
- 570:572 mutation Missing: In poxB8; reduced activity in vitro, not activated by lipids.
- 572 R→G: In poxB10; reduced activity in vivo and in vitro; may interact less with membranes.
3ey9A Structural basis for membrane binding and catalytic activation of the peripheral membrane enzyme pyruvate oxidase from escherichia coli (see paper)
32% identity, 89% coverage: 52:545/553 of query aligns to 2:524/571 of 3ey9A
- active site: V23 (≠ I73), G25 (= G75), D26 (≠ T76), S27 (= S77), L28 (≠ T78), E49 (= E99), S72 (≠ V122), F111 (≠ S161), Q112 (= Q162), G160 (≠ R210), L252 (≠ S302), A279 (≠ T329), V379 (= V428), G405 (= G454), M407 (= M456), D432 (vs. gap), N459 (= N480), V461 (≠ N482), L462 (= L483), F464 (≠ M485), V465 (≠ I486), E468 (= E489)
- binding flavin-adenine dinucleotide: G208 (= G258), S209 (≠ F259), G210 (= G260), A232 (≠ T282), L233 (≠ F283), R234 (= R284), T250 (≠ H300), G251 (= G301), I253 (≠ L303), G272 (= G322), T273 (≠ S323), Q274 (≠ S324), F275 (= F325), Y277 (≠ D327), D291 (= D340), I292 (= I341), S296 (≠ M345), G309 (= G358), D310 (≠ R359), I311 (≠ S360), T383 (≠ G432), F402 (≠ V451), N403 (≠ T452)
- binding magnesium ion: D432 (vs. gap), N459 (= N480)
- binding thiamine diphosphate: T24 (≠ P74), E49 (= E99), S72 (≠ V122), G76 (≠ R126), H79 (≠ N129), G380 (= G429), T381 (≠ E430), P382 (≠ N431), M407 (= M456), G431 (vs. gap), D432 (vs. gap), G433 (vs. gap), G434 (vs. gap), N459 (= N480), V461 (≠ N482), L462 (= L483), G463 (≠ A484)
Sites not aligning to the query:
4feeA High-resolution structure of pyruvate oxidase in complex with reaction intermediate 2-hydroxyethyl-thiamin diphosphate carbanion-enamine, crystal b (see paper)
30% identity, 88% coverage: 59:544/553 of query aligns to 10:533/586 of 4feeA
- binding flavin-adenine dinucleotide: H93 (≠ R141), G212 (= G258), I213 (≠ F259), G214 (= G260), T236 (= T282), Y237 (≠ F283), P238 (≠ R284), A254 (≠ H300), N255 (≠ G301), V257 (≠ L303), G276 (= G322), N277 (≠ S323), N278 (≠ S324), P280 (vs. gap), F281 (= F325), D298 (= D340), I299 (= I341), K303 (≠ M345), D317 (≠ R359), A318 (≠ S360), N390 (≠ G432), N409 (≠ V451)
- binding magnesium ion: D439 (≠ N480), N466 (vs. gap), Q468 (vs. gap)
- binding pyruvic acid: N255 (≠ G301), R256 (≠ S302)
- binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V386 (= V428), D388 (≠ E430), A412 (≠ G454), M414 (= M456), G438 (≠ N479), G440 (≠ R481), N466 (vs. gap), Q468 (vs. gap), Y469 (vs. gap), G470 (vs. gap), F471 (vs. gap), I472 (vs. gap)
Sites not aligning to the query:
2ezuA Pyruvate oxidase variant f479w in complex with reaction intermediate 2-acetyl-thiamin diphosphate (see paper)
30% identity, 88% coverage: 59:544/553 of query aligns to 10:533/585 of 2ezuA
- active site: I24 (= I73), G26 (= G75), G27 (≠ T76), S28 (= S77), I29 (≠ T78), E51 (= E99), S74 (≠ V122), F113 (≠ S161), Q114 (= Q162), E115 (= E163), V162 (≠ R210), R256 (≠ S302), E283 (≠ D327), V386 (= V428), A412 (≠ G454), M414 (= M456), D439 (≠ N480), N466 (vs. gap), Q468 (vs. gap), Y469 (vs. gap), W471 (= W498), I472 (vs. gap), E475 (vs. gap)
- binding flavin-adenine dinucleotide: H93 (≠ R141), G212 (= G258), I213 (≠ F259), G214 (= G260), T236 (= T282), Y237 (≠ F283), P238 (≠ R284), A254 (≠ H300), N255 (≠ G301), R256 (≠ S302), V257 (≠ L303), G276 (= G322), N277 (≠ S323), N278 (≠ S324), P280 (vs. gap), F281 (= F325), D298 (= D340), I299 (= I341), K303 (≠ M345), D317 (≠ R359), A318 (≠ S360), N409 (≠ V451)
- binding 2-acetyl-thiamine diphosphate: V386 (= V428), D388 (≠ E430), M414 (= M456), G438 (≠ N479), G440 (≠ R481), N466 (vs. gap), Q468 (vs. gap), Y469 (vs. gap), G470 (vs. gap), W471 (= W498), I472 (vs. gap)
- binding magnesium ion: D439 (≠ N480), N466 (vs. gap), Q468 (vs. gap)
Sites not aligning to the query:
2ez9A Pyruvate oxidase variant f479w in complex with reaction intermediate analogue 2-phosphonolactyl-thiamin diphosphate (see paper)
30% identity, 88% coverage: 59:544/553 of query aligns to 10:533/585 of 2ez9A
- active site: I24 (= I73), G26 (= G75), G27 (≠ T76), S28 (= S77), I29 (≠ T78), E51 (= E99), S74 (≠ V122), F113 (≠ S161), Q114 (= Q162), E115 (= E163), V162 (≠ R210), R256 (≠ S302), E283 (≠ D327), V386 (= V428), A412 (≠ G454), M414 (= M456), D439 (≠ N480), N466 (vs. gap), Q468 (vs. gap), Y469 (vs. gap), W471 (= W498), I472 (vs. gap), E475 (vs. gap)
- binding flavin-adenine dinucleotide: H93 (≠ R141), G212 (= G258), I213 (≠ F259), G214 (= G260), T236 (= T282), Y237 (≠ F283), P238 (≠ R284), A254 (≠ H300), N255 (≠ G301), R256 (≠ S302), V257 (≠ L303), G276 (= G322), N277 (≠ S323), N278 (≠ S324), P280 (vs. gap), F281 (= F325), D298 (= D340), I299 (= I341), K303 (≠ M345), D317 (≠ R359), A318 (≠ S360), N409 (≠ V451)
- binding magnesium ion: D439 (≠ N480), N466 (vs. gap), Q468 (vs. gap)
- binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-{(1s)-1-hydroxy-1-[(r)-hydroxy(methoxy)phosphoryl]ethyl}-5-(2-{[(s)-hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: V386 (= V428), D388 (≠ E430), M414 (= M456), G438 (≠ N479), G440 (≠ R481), N466 (vs. gap), Q468 (vs. gap), Y469 (vs. gap), G470 (vs. gap), W471 (= W498), I472 (vs. gap), E475 (vs. gap)
Sites not aligning to the query:
2ez8A Pyruvate oxidase variant f479w in complex with reaction intermediate 2-lactyl-thiamin diphosphate (see paper)
30% identity, 88% coverage: 59:544/553 of query aligns to 10:533/585 of 2ez8A
- active site: I24 (= I73), G26 (= G75), G27 (≠ T76), S28 (= S77), I29 (≠ T78), E51 (= E99), S74 (≠ V122), F113 (≠ S161), Q114 (= Q162), E115 (= E163), V162 (≠ R210), R256 (≠ S302), E283 (≠ D327), V386 (= V428), A412 (≠ G454), M414 (= M456), D439 (≠ N480), N466 (vs. gap), Q468 (vs. gap), Y469 (vs. gap), W471 (= W498), I472 (vs. gap), E475 (vs. gap)
- binding flavin-adenine dinucleotide: H93 (≠ R141), G212 (= G258), I213 (≠ F259), G214 (= G260), T236 (= T282), Y237 (≠ F283), P238 (≠ R284), A254 (≠ H300), N255 (≠ G301), R256 (≠ S302), V257 (≠ L303), G276 (= G322), N277 (≠ S323), N278 (≠ S324), P280 (vs. gap), F281 (= F325), D298 (= D340), I299 (= I341), K303 (≠ M345), D317 (≠ R359), A318 (≠ S360), N390 (≠ G432), N409 (≠ V451)
- binding magnesium ion: D439 (≠ N480), N466 (vs. gap), Q468 (vs. gap)
- binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-carboxy-1-hydroxyethyl)-5-(2-{[hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: D388 (≠ E430), M414 (= M456), G438 (≠ N479), G440 (≠ R481), N466 (vs. gap), Q468 (vs. gap), Y469 (vs. gap), G470 (vs. gap), W471 (= W498), I472 (vs. gap)
Sites not aligning to the query:
2ez4B Pyruvate oxidase variant f479w (see paper)
30% identity, 88% coverage: 59:544/553 of query aligns to 10:533/585 of 2ez4B
- active site: I24 (= I73), G26 (= G75), G27 (≠ T76), S28 (= S77), I29 (≠ T78), E51 (= E99), S74 (≠ V122), F113 (≠ S161), Q114 (= Q162), E115 (= E163), V162 (≠ R210), R256 (≠ S302), E283 (≠ D327), V386 (= V428), A412 (≠ G454), M414 (= M456), D439 (≠ N480), N466 (vs. gap), Q468 (vs. gap), Y469 (vs. gap), W471 (= W498), I472 (vs. gap), E475 (vs. gap)
- binding flavin-adenine dinucleotide: H93 (≠ R141), G212 (= G258), I213 (≠ F259), G214 (= G260), T236 (= T282), Y237 (≠ F283), P238 (≠ R284), A254 (≠ H300), N255 (≠ G301), R256 (≠ S302), V257 (≠ L303), G276 (= G322), N277 (≠ S323), N278 (≠ S324), P280 (vs. gap), F281 (= F325), D298 (= D340), I299 (= I341), K303 (≠ M345), D317 (≠ R359), A318 (≠ S360), N409 (≠ V451)
- binding magnesium ion: D439 (≠ N480), N466 (vs. gap), Q468 (vs. gap)
- binding phosphate ion: W471 (= W498), E475 (vs. gap)
- binding thiamine diphosphate: D388 (≠ E430), A412 (≠ G454), M414 (= M456), G438 (≠ N479), D439 (≠ N480), G440 (≠ R481), G441 (≠ N482), N466 (vs. gap), Q468 (vs. gap), Y469 (vs. gap), G470 (vs. gap), W471 (= W498), I472 (vs. gap)
Sites not aligning to the query:
1powA The refined structures of a stabilized mutant and of wild-type pyruvate oxidase from lactobacillus plantarum (see paper)
30% identity, 88% coverage: 59:544/553 of query aligns to 10:533/585 of 1powA
- active site: I24 (= I73), G26 (= G75), G27 (≠ T76), S28 (= S77), I29 (≠ T78), E51 (= E99), S74 (≠ V122), F113 (≠ S161), Q114 (= Q162), E115 (= E163), V162 (≠ R210), R256 (≠ S302), E283 (≠ D327), V386 (= V428), A412 (≠ G454), M414 (= M456), D439 (≠ N480), N466 (vs. gap), Q468 (vs. gap), Y469 (vs. gap), F471 (vs. gap), I472 (vs. gap), E475 (vs. gap)
- binding flavin-adenine dinucleotide: H93 (≠ R141), G212 (= G258), I213 (≠ F259), G214 (= G260), T236 (= T282), Y237 (≠ F283), A254 (≠ H300), V257 (≠ L303), G276 (= G322), N277 (≠ S323), N278 (≠ S324), Y279 (vs. gap), P280 (vs. gap), F281 (= F325), D298 (= D340), I299 (= I341), K303 (≠ M345), D317 (≠ R359), A318 (≠ S360), N409 (≠ V451)
- binding magnesium ion: D439 (≠ N480), N466 (vs. gap), Q468 (vs. gap)
- binding thiamine diphosphate: D388 (≠ E430), M414 (= M456), G440 (≠ R481), N466 (vs. gap), Q468 (vs. gap), Y469 (vs. gap), G470 (vs. gap), F471 (vs. gap), I472 (vs. gap)
Sites not aligning to the query:
6lpiB Crystal structure of ahas holo-enzyme (see paper)
30% identity, 90% coverage: 53:548/553 of query aligns to 7:520/539 of 6lpiB
- active site: I27 (= I73), G29 (= G75), G30 (≠ T76), S31 (= S77), I32 (≠ T78), E53 (= E99), C76 (≠ V122), F115 (≠ S161), Q116 (= Q162), E117 (= E163), K165 (≠ R210), M256 (≠ S302), A283 (≠ T329), V375 (= V428), G401 (= G454), M403 (= M456), D428 (vs. gap), N455 (= N480), A457 (≠ N482), L458 (= L483), L460 (≠ M485), V461 (≠ I486), Q464 (≠ E489)
- binding flavin-adenine dinucleotide: R155 (= R201), G212 (= G258), G213 (≠ F259), G214 (= G260), T236 (= T282), L237 (≠ F283), M238 (≠ R284), L254 (≠ H300), M256 (≠ S302), H257 (≠ L303), G276 (= G322), A277 (≠ S323), R278 (≠ S324), D280 (≠ S326), R282 (≠ L328), A283 (≠ T329), D300 (= D340), I301 (= I341), D319 (≠ R359), V320 (≠ S360), M380 (≠ W433), G398 (≠ V451)
- binding magnesium ion: D428 (vs. gap), N455 (= N480)
- binding thiamine diphosphate: E53 (= E99), C76 (≠ V122), P79 (= P125), G376 (= G429), Q377 (≠ E430), H378 (≠ N431), G401 (= G454), M403 (= M456), G427 (vs. gap), D428 (vs. gap), G429 (vs. gap), S430 (vs. gap), M433 (vs. gap), N455 (= N480), A457 (≠ N482), L458 (= L483), G459 (≠ A484), L460 (≠ M485), V461 (≠ I486)
1y9dD Pyruvate oxidase variant v265a from lactobacillus plantarum (see paper)
29% identity, 88% coverage: 59:544/553 of query aligns to 10:508/560 of 1y9dD
- active site: I24 (= I73), G26 (= G75), G27 (≠ T76), S28 (= S77), I29 (≠ T78), E51 (= E99), S74 (≠ V122), E108 (= E163), V155 (≠ R210), R241 (≠ S302), V361 (= V428), A387 (≠ G454), M389 (= M456), D414 (≠ N480), N441 (vs. gap), Q443 (vs. gap), Y444 (vs. gap), F446 (vs. gap), I447 (vs. gap), E450 (vs. gap)
- binding flavin-adenine dinucleotide: I198 (≠ F259), G199 (= G260), T221 (= T282), P223 (≠ R284), G261 (= G322), N262 (≠ S323), N263 (≠ S324), D273 (= D340), I274 (= I341), K278 (≠ M345), D292 (≠ R359), A293 (≠ S360)
- binding magnesium ion: D414 (≠ N480), N441 (vs. gap), Q443 (vs. gap)
- binding thiamine diphosphate: E51 (= E99), S74 (≠ V122), P77 (= P125), H81 (≠ N129), D363 (≠ E430), M389 (= M456), G413 (≠ N479), G415 (≠ R481), N441 (vs. gap), Q443 (vs. gap), Y444 (vs. gap), G445 (vs. gap), F446 (vs. gap), I447 (vs. gap)
Sites not aligning to the query:
1jscA Crystal structure of the catalytic subunit of yeast acetohydroxyacid synthase: a target for herbicidal inhibitors (see paper)
29% identity, 90% coverage: 51:550/553 of query aligns to 9:541/541 of 1jscA
- active site: Y31 (≠ I73), G33 (= G75), G34 (≠ T76), A35 (≠ S77), I36 (≠ T78), E57 (= E99), T80 (≠ V122), F119 (≠ S161), Q120 (= Q162), E121 (= E163), K169 (≠ R210), M263 (≠ S302), V290 (vs. gap), V406 (= V428), G432 (= G454), M434 (= M456), D459 (vs. gap), N486 (vs. gap), E488 (vs. gap), K540 (≠ P549)
- binding dihydrogenphosphate ion: G33 (= G75), G34 (≠ T76), Q120 (= Q162)
- binding flavin-adenine dinucleotide: R159 (= R201), G216 (= G258), A217 (≠ F259), G218 (= G260), N221 (≠ E263), T243 (= T282), L244 (≠ F283), L261 (≠ H300), G262 (= G301), H264 (≠ L303), G283 (= G322), A284 (≠ S323), R285 (≠ S324), D287 (≠ S326), R289 (vs. gap), V290 (vs. gap), E316 (≠ D340), V317 (≠ I341), N321 (≠ M345), G334 (= G358), D335 (≠ R359), A336 (≠ S360)
- binding magnesium ion: D459 (vs. gap), N486 (vs. gap)
- binding thiamine diphosphate: Y31 (≠ I73), P32 (= P74), E57 (= E99), P83 (= P125), V406 (= V428), G407 (= G429), Q408 (≠ E430), H409 (≠ N431), M434 (= M456), D459 (vs. gap), A460 (vs. gap), S461 (≠ N480), N486 (vs. gap)
6bd3A Saccharomyces cerevisiae acetohydroxyacid synthase
29% identity, 90% coverage: 51:550/553 of query aligns to 9:538/538 of 6bd3A
- active site: Y31 (≠ I73), G33 (= G75), G34 (≠ T76), A35 (≠ S77), I36 (≠ T78), E57 (= E99), T80 (≠ V122), F119 (≠ S161), Q120 (= Q162), E121 (= E163), K169 (≠ R210), R225 (vs. gap), M261 (≠ S302), V288 (vs. gap), V404 (= V428), L429 (≠ W453), G430 (= G454), M432 (= M456), D457 (vs. gap), N484 (vs. gap), L498 (≠ F510), G503 (= G515), L504 (≠ G516), K537 (≠ P549)
- binding flavin-adenine dinucleotide: R159 (= R201), G214 (= G258), A215 (≠ F259), G216 (= G260), N219 (≠ E263), T241 (= T282), L242 (≠ F283), Q243 (≠ R284), L259 (≠ H300), G260 (= G301), H262 (≠ L303), G281 (= G322), A282 (≠ S323), R283 (≠ S324), D285 (≠ S326), R287 (vs. gap), V288 (vs. gap), E314 (≠ D340), V315 (≠ I341), D333 (≠ R359), A334 (≠ S360)
- binding 2-acetyl-thiamine diphosphate: P32 (= P74), E57 (= E99), P83 (= P125)
- binding magnesium ion: D457 (vs. gap), N484 (vs. gap)
- binding oxygen molecule: A35 (≠ S77), T80 (≠ V122), S81 (≠ A123), Q120 (= Q162)
- binding thiamine diphosphate: V404 (= V428), G405 (= G429), Q406 (≠ E430), H407 (≠ N431), G430 (= G454), M432 (= M456), D457 (vs. gap), A458 (vs. gap), S459 (≠ N480), M462 (≠ L483), N484 (vs. gap)
6bd9A Saccharomyces cerevisiae acetohydroxyacid synthase
29% identity, 90% coverage: 51:550/553 of query aligns to 9:542/542 of 6bd9A
- active site: Y31 (≠ I73), G33 (= G75), G34 (≠ T76), A35 (≠ S77), I36 (≠ T78), E57 (= E99), T80 (≠ V122), F119 (≠ S161), Q120 (= Q162), E121 (= E163), K169 (≠ R210), R228 (vs. gap), M264 (≠ S302), V291 (vs. gap), V407 (= V428), L432 (≠ W453), G433 (= G454), M435 (= M456), D460 (vs. gap), N487 (vs. gap), E489 (vs. gap), L502 (≠ F510), G507 (= G515), L508 (≠ G516), K541 (≠ P549)
- binding flavin-adenine dinucleotide: R159 (= R201), G217 (= G258), A218 (≠ F259), G219 (= G260), N222 (≠ E263), T244 (= T282), L245 (≠ F283), L262 (≠ H300), G263 (= G301), H265 (≠ L303), G284 (= G322), A285 (≠ S323), R286 (≠ S324), D288 (≠ S326), R290 (vs. gap), V291 (vs. gap), E317 (≠ D340), V318 (≠ I341), N322 (≠ M345), G335 (= G358), D336 (≠ R359), A337 (≠ S360)
- binding magnesium ion: D460 (vs. gap), N487 (vs. gap)
- binding oxygen molecule: G34 (≠ T76), T80 (≠ V122), Q120 (= Q162), A461 (vs. gap), Q494 (≠ L502)
- binding pyruvic acid: G33 (= G75), G34 (≠ T76), G34 (≠ T76), A35 (≠ S77), Q120 (= Q162)
- binding thiamine diphosphate: P32 (= P74), E57 (= E99), V407 (= V428), G408 (= G429), Q409 (≠ E430), H410 (≠ N431), G433 (= G454), M435 (= M456), G459 (vs. gap), D460 (vs. gap), A461 (vs. gap), S462 (≠ N480), M465 (≠ L483), N487 (vs. gap)
5imsA Saccharomyces cerevisiae acetohydroxyacid synthase
29% identity, 90% coverage: 51:548/553 of query aligns to 9:540/541 of 5imsA
- active site: Y31 (≠ I73), G33 (= G75), G34 (≠ T76), A35 (≠ S77), I36 (≠ T78), E57 (= E99), T80 (≠ V122), F119 (≠ S161), Q120 (= Q162), E121 (= E163), K169 (≠ R210), R229 (vs. gap), M265 (≠ S302), V292 (vs. gap), V408 (= V428), L433 (≠ W453), G434 (= G454), M436 (= M456), D461 (vs. gap), N488 (vs. gap), E490 (vs. gap), L502 (≠ F510), G507 (= G515), L508 (≠ G516)
- binding flavin-adenine dinucleotide: R159 (= R201), G218 (= G258), A219 (≠ F259), G220 (= G260), N223 (≠ E263), T245 (= T282), L246 (≠ F283), L263 (≠ H300), G264 (= G301), H266 (≠ L303), G285 (= G322), A286 (≠ S323), R287 (≠ S324), D289 (≠ S326), R291 (vs. gap), V292 (vs. gap), E318 (≠ D340), V319 (≠ I341), N323 (≠ M345), D337 (≠ R359), A338 (≠ S360)
- binding magnesium ion: D461 (vs. gap), N488 (vs. gap)
- binding oxygen molecule: G34 (≠ T76), T80 (≠ V122), Q120 (= Q162)
- binding thiamine diphosphate: P32 (= P74), E57 (= E99), V408 (= V428), G409 (= G429), Q410 (≠ E430), H411 (≠ N431), G434 (= G454), M436 (= M456), G460 (vs. gap), D461 (vs. gap), A462 (vs. gap), S463 (≠ N480), M466 (≠ L483), N488 (vs. gap)
Sites not aligning to the query:
1v5gA Crystal structure of the reaction intermediate between pyruvate oxidase containing fad and tpp, and substrate pyruvate (see paper)
26% identity, 86% coverage: 62:538/553 of query aligns to 13:524/589 of 1v5gA
- binding flavin-adenine dinucleotide: G212 (= G258), I213 (≠ F259), G214 (= G260), T236 (= T282), G237 (≠ F283), K238 (≠ R284), T254 (≠ H300), Y255 (≠ G301), R256 (≠ S302), V257 (≠ L303), G276 (= G322), S277 (= S323), N278 (≠ S324), F279 (= F325), F281 (vs. gap), D298 (= D340), I299 (= I341), M303 (= M345), D317 (≠ R359), A318 (≠ S360), P409 (≠ V451)
- binding 2-acetyl-thiamine diphosphate: V386 (= V428), N388 (≠ E430), M414 (= M456), G438 (vs. gap), G440 (vs. gap), A441 (vs. gap), N466 (= N480), E468 (≠ N482), Y469 (≠ L483), A470 (= A484), F471 (≠ M485), I472 (= I486)
- binding magnesium ion: D439 (vs. gap), N466 (= N480), E468 (≠ N482)
1v5fA Crystal structure of pyruvate oxidase complexed with fad and tpp, from aerococcus viridans (see paper)
26% identity, 86% coverage: 62:538/553 of query aligns to 13:524/589 of 1v5fA
- binding flavin-adenine dinucleotide: G212 (= G258), I213 (≠ F259), G214 (= G260), T236 (= T282), G237 (≠ F283), K238 (≠ R284), T254 (≠ H300), Y255 (≠ G301), R256 (≠ S302), V257 (≠ L303), G276 (= G322), S277 (= S323), N278 (≠ S324), F279 (= F325), P280 (≠ S326), F281 (vs. gap), D298 (= D340), I299 (= I341), M303 (= M345), D317 (≠ R359), A318 (≠ S360), P409 (≠ V451)
- binding magnesium ion: D439 (vs. gap), N466 (= N480)
- binding thiamine diphosphate: N388 (≠ E430), S389 (≠ N431), M414 (= M456), G438 (vs. gap), G440 (vs. gap), N466 (= N480), Y469 (≠ L483), A470 (= A484), F471 (≠ M485), I472 (= I486)
2djiA Crystal structure of pyruvate oxidase from aerococcus viridans containing fad (see paper)
26% identity, 86% coverage: 62:538/553 of query aligns to 14:525/590 of 2djiA
- active site: I25 (= I73), S27 (≠ G75), G28 (≠ T76), T29 (≠ S77), L30 (≠ T78), E52 (= E99), S75 (≠ V122), F114 (≠ S161), Q115 (= Q162), G163 (≠ R210), R257 (≠ S302), E284 (vs. gap), V387 (= V428), A413 (≠ G454), M415 (= M456), D440 (vs. gap), N467 (= N480), E469 (≠ N482), Y470 (≠ L483), F472 (≠ M485), I473 (= I486), K476 (≠ E489)
- binding flavin-adenine dinucleotide: G213 (= G258), I214 (≠ F259), G215 (= G260), T237 (= T282), G238 (≠ F283), K239 (≠ R284), T255 (≠ H300), Y256 (≠ G301), R257 (≠ S302), V258 (≠ L303), G277 (= G322), S278 (= S323), N279 (≠ S324), F280 (= F325), P281 (≠ S326), F282 (vs. gap), D299 (= D340), I300 (= I341), M304 (= M345), D318 (≠ R359), A319 (≠ S360), P410 (≠ V451)
Sites not aligning to the query:
7tzzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase p197t mutant in complex with bispyribac-sodium (see paper)
30% identity, 89% coverage: 52:545/553 of query aligns to 12:554/582 of 7tzzA
- binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: M266 (≠ S302), R292 (vs. gap), W489 (vs. gap)
- binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V428), G401 (= G429), Q402 (≠ E430), H403 (≠ N431), G426 (= G454), M428 (= M456), G452 (vs. gap), D453 (vs. gap), G454 (vs. gap), S455 (vs. gap), L483 (= L483), G484 (≠ A484), M485 (= M485), V486 (≠ I486)
- binding flavin-adenine dinucleotide: R161 (= R201), G222 (= G258), G223 (≠ F259), G224 (= G260), T246 (= T282), L247 (≠ F283), M248 (≠ R284), M263 (≠ C299), L264 (≠ H300), M266 (≠ S302), H267 (≠ L303), G286 (= G322), R288 (≠ S324), V293 (vs. gap), D310 (= D340), I311 (= I341), D329 (≠ R359), V330 (≠ S360), M405 (≠ W433), G423 (≠ V451)
- binding magnesium ion: A37 (≠ S77), T82 (≠ V122), S83 (≠ A123), Q122 (= Q162), Y381 (= Y409), D453 (vs. gap), M458 (vs. gap), Q461 (vs. gap), N480 (= N480), H482 (≠ N482), K533 (≠ P524)
Sites not aligning to the query:
8et4A Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with amidosulfuron (see paper)
30% identity, 89% coverage: 52:545/553 of query aligns to 12:554/582 of 8et4A
- binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V428), G401 (= G429), Q402 (≠ E430), H403 (≠ N431), G426 (= G454), M428 (= M456), G452 (vs. gap), D453 (vs. gap), G454 (vs. gap), S455 (vs. gap), M458 (vs. gap), N480 (= N480), H482 (≠ N482), L483 (= L483), G484 (≠ A484), M485 (= M485), V486 (≠ I486)
- binding flavin-adenine dinucleotide: R161 (= R201), G222 (= G258), G223 (≠ F259), G224 (= G260), T246 (= T282), L247 (≠ F283), M248 (≠ R284), L264 (≠ H300), M266 (≠ S302), H267 (≠ L303), G286 (= G322), V287 (≠ S323), R288 (≠ S324), D290 (≠ S326), R292 (vs. gap), V293 (vs. gap), D310 (= D340), I311 (= I341), D329 (≠ R359), V330 (≠ S360), M405 (≠ W433), G423 (≠ V451)
- binding magnesium ion: F370 (≠ L402), D453 (vs. gap), M458 (vs. gap), Q461 (vs. gap), N480 (= N480), H482 (≠ N482), K533 (≠ P524)
- binding N-{[(4,6-dimethoxypyrimidin-2-yl)carbamoyl]sulfamoyl}-N-methylmethanesulfonamide: M266 (≠ S302), R292 (vs. gap), M485 (= M485), W489 (vs. gap)
Sites not aligning to the query:
5wj1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a triazolopyrimidine herbicide, penoxsulam (see paper)
30% identity, 89% coverage: 52:545/553 of query aligns to 12:554/582 of 5wj1A
- active site: Y33 (≠ I73), G35 (= G75), G36 (≠ T76), A37 (≠ S77), S38 (≠ T78), E59 (= E99), T82 (≠ V122), F121 (≠ S161), Q122 (= Q162), E123 (= E163), K171 (≠ R210), M266 (≠ S302), V293 (vs. gap), V400 (= V428), G426 (= G454), M428 (= M456), D453 (vs. gap), N480 (= N480), H482 (≠ N482), L483 (= L483), M485 (= M485), V486 (≠ I486), W489 (vs. gap)
- binding flavin-adenine dinucleotide: R161 (= R201), G222 (= G258), G223 (≠ F259), G224 (= G260), T246 (= T282), L247 (≠ F283), M248 (≠ R284), M263 (≠ C299), L264 (≠ H300), G286 (= G322), R288 (≠ S324), V293 (vs. gap), D310 (= D340), I311 (= I341), D329 (≠ R359), V330 (≠ S360), M405 (≠ W433), G423 (≠ V451), G424 (≠ T452)
- binding magnesium ion: D453 (vs. gap), N480 (= N480), H482 (≠ N482)
- binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: M266 (≠ S302), D291 (= D327), R292 (vs. gap), M485 (= M485), W489 (vs. gap)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V428), G401 (= G429), Q402 (≠ E430), H403 (≠ N431), M428 (= M456), D453 (vs. gap), G454 (vs. gap), S455 (vs. gap), M458 (vs. gap), N480 (= N480), H482 (≠ N482), L483 (= L483), G484 (≠ A484), M485 (= M485), V486 (≠ I486)
Sites not aligning to the query:
Query Sequence
>WP_010876115.1 NCBI__GCF_000008645.1:WP_010876115.1
MADFRCTVCNYIFHEEADGEFDSLPADWRCPVCNAPRTAFVRVPSTLEEGGRTVSEVFIS
QLAAWGLKYVFGIPGTSTLGLVDALRRNDEIRYIQVRHEAAAAFMASSYGKLTGQPAVCM
AVAGPRASNLITGLLDAALDRAPVLAVTGHVETYRIGTGASQEIDQHSLFESFSVYNMTL
VSPDEAAEIAREAVKHAILERGVSHVDVPRDVQTMECTAPVKPLRGMMAEAAVTPPRNRL
RAAADLINRAERPVIIAGFGALEAADSVVELAERIGAAIVSTFRGKGIVDNDYPLYLGCH
GSLGSAAAAEAVRKADLLLVIGSSFSDLTRIPPRRTLQVDIDPMMVARRHPVEQGLTGRS
SLIVQELISMVEEKERGPYLAELGELRDEWLGLLESEADPSLRPIRPQYIISVLNRELDD
DAIITLDVGENGWWFGRNFQMKSTRGFSFQVTWGSMGFGLPAAIAAQLEFPDRQVVCINN
RNLAMIMQEQRVEGFPVWQTELQDCDFAGFAENCGGRGLRVDDPGELEDSVREALGTDGP
VLVDIETDPRRFI
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory