SitesBLAST

7bkbK Formate dehydrogenase - heterodisulfide reductase - formylmethanofuran dehydrogenase complex from methanospirillum hungatei (hexameric, composite structure) (see paper)
33% identity, 98% coverage: 1:325/332 of query aligns to 16:358/386 of 7bkbK

query
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7bkbK

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binding iron/sulfur cluster: C36 (= C21), T37 (= T22), C39 (= C24), C42 (= C27), C46 (= C31), P47 (= P32), I51 (= I36), C77 (= C61), S78 (≠ V62), Y79 (≠ L63), C80 (= C64), G81 (= G65), C83 (= C67), C87 (= C71), L92 (≠ I76), I119 (= I100), C124 (= C105), V125 (≠ F106), C127 (= C108), C130 (= C111), C134 (= C115), I139 (≠ L120), C173 (= C144), T174 (≠ I145), K175 (≠ Y146), C176 (= C147), C179 (= C150), C183 (= C154), I186 (≠ S157), C211 (= C184), D212 (≠ L185), C214 (= C187), N215 (≠ G188), C217 (= C190), C221 (= C194), P222 (= P195), V241 (= V220), C248 (= C227), C249 (≠ A228), C251 (= C230), C254 (= C233), C258 (= C237), P259 (= P238), I263 (= I242), V265 (= V244), I273 (= I252), C280 (= C259), P281 (≠ R260), C284 (= C264), T286 (≠ A266), C287 (= C267), C291 (= C271), C322 (= C290), I323 (≠ V291), L324 (≠ F292), C325 (= C293), G326 (= G294), C328 (= C296), C332 (= C300), P333 (= P301)

7bkcK Formate dehydrogenase - heterodisulfide reductase - formylmethanofuran dehydrogenase complex from methanospirillum hungatei (dimeric, composite structure) (see paper)
31% identity, 69% coverage: 98:325/332 of query aligns to 29:293/321 of 7bkcK

query
sites
7bkcK

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binding iron/sulfur cluster: C36 (= C105), T37 (≠ F106), C39 (= C108), C42 (= C111), C46 (= C115), P47 (= P116), I51 (≠ L120), C77 (= C144), S78 (≠ I145), Y79 (= Y146), C80 (= C147), G81 (= G148), C83 (= C150), C87 (= C154), L92 (≠ I159), I119 (≠ V179), C124 (= C184), V125 (≠ L185), C127 (= C187), C130 (= C190), C134 (= C194), P135 (= P195), I139 (= I199), I178 (≠ T222), C183 (= C227), C184 (≠ A228), T185 (≠ H229), C186 (= C230), R187 (≠ G231), C189 (= C233), C193 (= C237), P194 (= P238), I198 (= I242), I208 (= I252), C215 (= C259), P216 (≠ R260), C219 (= C264), T221 (≠ A266), C222 (= C267), C226 (= C271), C257 (= C290), I258 (≠ V291), L259 (≠ F292), C260 (= C293), G261 (= G294), C263 (= C296), C267 (= C300), P268 (= P301)

8oh5C Cryo-em structure of the electron bifurcating transhydrogenase stnabc complex from sporomusa ovata (state 2) (see paper)
36% identity, 28% coverage: 57:148/332 of query aligns to 609:696/1172 of 8oh5C

query
sites
8oh5C

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binding iron/sulfur cluster: C613 (= C61), V614 (= V62), L615 (= L63), C616 (= C64), G617 (= G65), C619 (= C67), C623 (= C71), I643 (≠ P92), C656 (= C105), I657 (≠ F106), S658 (≠ L107), C659 (= C108), G660 (= G109), C662 (= C111), C666 (= C115), G669 (≠ N118), A670 (= A119), C692 (= C144), Y694 (= Y146), C695 (= C147)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 199, 201, 202, 203, 222, 223, 229, 230, 235, 239, 264, 265, 285, 288, 332, 471, 477, 479
binding fe2/s2 (inorganic) cluster: 36, 45, 47, 48, 50, 64
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 330, 331, 332, 333, 353, 354, 355, 432, 433, 434, 477
binding iron/sulfur cluster: 96, 98, 100, 102, 104, 108, 147, 149, 151, 155, 156, 164, 170, 480, 562, 563, 565, 567, 573, 699, 725, 727, 728, 853

7p8nB Tmhydabc- t. Maritima hydrogenase with bridge closed (see paper)
48% identity, 18% coverage: 17:77/332 of query aligns to 563:613/613 of 7p8nB

query
sites
7p8nB

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binding iron/sulfur cluster: C567 (= C21), C570 (= C24), G571 (= G25), C573 (= C27), C577 (= C31), Y590 (≠ V54), I592 (≠ F56), C597 (= C61), K599 (≠ L63), C600 (= C64), G601 (= G65), C603 (= C67), C607 (= C71)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 31, 33, 34, 36, 67, 68, 69, 70, 71
binding flavin mononucleotide: 185, 186, 187, 213, 215, 216, 217, 301, 304, 305, 306, 340, 341, 521, 522
binding iron/sulfur cluster: 320, 473, 474, 475, 476, 477, 480, 519, 520, 522, 523, 560
binding zinc ion: 457, 544, 549, 554

7plmA Cryoem reconstruction of pyruvate ferredoxin oxidoreductase (pfor) in anaerobic conditions (see paper)
27% identity, 40% coverage: 32:165/332 of query aligns to 630:764/1177 of 7plmA

query
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7plmA

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binding iron/sulfur cluster: P657 (vs. gap), W659 (vs. gap), C664 (= C61), I665 (≠ V62), Q666 (≠ L63), C667 (= C64), N668 (≠ G65), C670 (= C67), C674 (= C71), P675 (≠ I72), I679 (= I76), I708 (≠ L98), C715 (= C105), M716 (≠ F106), G717 (≠ L107), C718 (= C108), G719 (= G109), C721 (= C111), C725 (= C115), P726 (= P116), A731 (≠ S131), L732 (= L132)

Sites not aligning to the query:

binding calcium ion: 950, 952, 1026, 1028
binding magnesium ion: 930, 958, 960, 962
binding iron/sulfur cluster: 458, 781, 782, 785, 787, 810, 962, 1038, 1039
binding thiamine diphosphate: 27, 28, 29, 63, 787, 808, 809, 810, 839, 929, 930, 931, 960, 961, 962, 963, 964

2c3uA Crystal structure of pyruvate-ferredoxin oxidoreductase from desulfovibrio africanus, oxygen inhibited form (see paper)
26% identity, 40% coverage: 32:165/332 of query aligns to 654:793/1231 of 2c3uA

query
sites
2c3uA

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F

K

E

M

K

N

R

R

G

V

V

S

A

F

I

N

N

-

V

-
x
P

-

Q

-
x
W

-

V

K

P

E

E

K

N

C
|
C

V
x
I

L

Q

C
|
C

G
x
N

L

Q

C
|
C

A

A

S

F

V

V

C
|
C

I
x
P

F

H

G

S

A
|
A

I
|
I

-

L

-

P

-

V

-

L

-

A

-

K

-

E

-

L

-

V

-

G

-

A

-

P

-

A

-

N

-

F

-

T

D

A

L

L

Q

E

K

A

D

K

G

G

K

K

S

E

I

L

R

K

G

G

A

-

D

-

E

-

Y

-

P

-

F

-

W

Y

D

K

F

F

K

R

L

I

E

Q

I

I

D

N

D

T

E

L

K

D

C
|
C

F
x
M

L
x
G

C
|
C

G
|
G

N

N

C
|
C

A

A

D

D

A

I

C
|
C

P
|
P

R

-

N

-

A

-

L

-

T

-

I

-

R

-

D

-

L

-

P

P

E

K

R

E

K

K

S
x
A

L
|
L

V

V

K

M

G

Q

E

P

I

L

N

D

V

T

S

Q

M

R

E

D

K

A

C

Q

I

V

Y

P

C

N

G

L

E

E

C

Y

A

A

M

R

C

I

P

P

A

V

S

K

A

S

I

-

E

E

I

V

S

L

W

P

R

R

D

D

binding iron/sulfur cluster: P681 (vs. gap), W683 (vs. gap), C688 (= C61), I689 (≠ V62), C691 (= C64), N692 (≠ G65), C694 (= C67), C698 (= C71), P699 (≠ I72), A702 (= A75), I703 (= I76), C744 (= C105), M745 (≠ F106), G746 (≠ L107), C747 (= C108), G748 (= G109), C750 (= C111), C754 (= C115), P755 (= P116), A760 (≠ S131), L761 (= L132)

Sites not aligning to the query:

active site: 30, 63, 113, 995
binding 2-(3-{[4-(hydroxyamino)-2-methylpyrimidin-5-yl]methyl}-4-methyl-2,3-dihydro-1,3-thiazol-5-yl)ethyl trihydrogen diphosphate: 28, 29, 63, 87, 816, 837, 839, 868, 869, 961, 962, 963, 964, 992, 993, 994, 995, 996
binding calcium ion: 982, 984, 1055, 1056, 1058, 1060, 1062
binding magnesium ion: 962, 990, 992
binding pyruvic acid: 30, 113, 122, 995
binding iron/sulfur cluster: 458, 811, 814, 816, 839, 1070, 1071

2c3pA Crystal structure of the free radical intermediate of pyruvate:ferredoxin oxidoreductase from desulfovibrio africanus (see paper)
26% identity, 40% coverage: 32:165/332 of query aligns to 654:793/1231 of 2c3pA

query
sites
2c3pA

P

P

V

V

N

S

A

A

I

F

D

E

R

A

A

D

P

G

L

R

L

F

P

P

I

L

A

G

R

T

G

S

L

Q

I

F

K

E

M

K

N

R

R

G

V

V

S

A

F

I

N

N

-

V

-
x
P

-

Q

-
x
W

-

V

K

P

E

E

K

N

C
|
C

V
x
I

L
x
Q

C
|
C

G
x
N

L

Q

C
|
C

A

A

S

F

V

V

C
|
C

I
x
P

F

H

G

S

A
|
A

I
|
I

-

L

-

P

-

V

-

L

-

A

-

K

-

E

-

L

-

V

-

G

-

A

-

P

-

A

-

N

-

F

-

T

D

A

L

L

Q

E

K

A

D

K

G

G

K

K

S

E

I

L

R

K

G

G

A

-

D

-

E

-

Y

-

P

-

F

-

W

Y

D

K

F

F

K

R

L

I

E

Q

I

I

D

N

D

T

E

L

K

D

C
|
C

F
x
M

L
x
G

C
|
C

G
|
G

N

N

C
|
C

A

A

D

D

A

I

C
|
C

P
|
P

R

-

N

-

A

-

L

-

T

-

I

-

R

-

D

-

L

-

P

P

E

K

R

E

K

K

S
x
A

L
|
L

V

V

K

M

G

Q

E

P

I

L

N

D

V

T

S

Q

M

R

E

D

K

A

C

Q

I

V

Y

P

C

N

G

L

E

E

C

Y

A

A

M

R

C

I

P

P

A

V

S

K

A

S

I

-

E

E

I

V

S

L

W

P

R

R

D

D

binding iron/sulfur cluster: P681 (vs. gap), W683 (vs. gap), C688 (= C61), I689 (≠ V62), Q690 (≠ L63), C691 (= C64), N692 (≠ G65), C694 (= C67), C698 (= C71), P699 (≠ I72), A702 (= A75), I703 (= I76), C744 (= C105), M745 (≠ F106), G746 (≠ L107), C747 (= C108), G748 (= G109), C750 (= C111), C754 (= C115), P755 (= P116), A760 (≠ S131), L761 (= L132)

Sites not aligning to the query:

active site: 30, 63, 113, 995
binding 1-(2-{(2s,4r,5r)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-[(1s)-1-carboxy-1-hydroxyethyl]-4-methyl-1,3-thiazolidin-5-yl}ethoxy)-1,1,3,3-tetrahydroxy-1lambda~5~-diphosphox-1-en-2-ium 3-oxide: 28, 29, 30, 63, 87, 113, 816, 837, 838, 839, 868, 869, 961, 962, 963, 992, 993, 994, 995, 996
binding calcium ion: 982, 983, 984, 1055, 1056, 1058, 1060, 1062
binding magnesium ion: 962, 990, 992
binding iron/sulfur cluster: 458, 810, 811, 814, 816, 839, 994, 1070, 1071

2c3oA Crystal structure of the free radical intermediate of pyruvate:ferredoxin oxidoreductase from desulfovibrio africanus (see paper)
26% identity, 40% coverage: 32:165/332 of query aligns to 654:793/1231 of 2c3oA

query
sites
2c3oA

P

P

V

V

N

S

A

A

I

F

D

E

R

A

A

D

P

G

L

R

L

F

P

P

I

L

A

G

R

T

G

S

L

Q

I

F

K

E

M

K

N

R

R

G

V

V

S

A

F

I

N

N

-

V

-
x
P

-

Q

-

W

-

V

K

P

E

E

K

N

C
|
C

V
x
I

L

Q

C
|
C

G
x
N

L

Q

C
|
C

A

A

S

F

V

V

C
|
C

I
x
P

F

H

G

S

A
|
A

I
|
I

-

L

-

P

-

V

-

L

-

A

-

K

-

E

-

L

-

V

-

G

-

A

-

P

-

A

-

N

-

F

-

T

D

A

L

L

Q

E

K

A

D

K

G

G

K

K

S

E

I

L

R

K

G

G

A

-

D

-

E

-

Y

-

P

-

F

-

W

Y

D

K

F

F

K

R

L

I

E

Q

I

I

D

N

D

T

E

L

K

D

C
|
C

F

M

L
x
G

C
|
C

G
|
G

N
|
N

C
|
C

A

A

D

D

A

I

C
|
C

P

P

R

-

N

-

A

-

L

-

T

-

I

-

R

-

D

-

L

-

P

P

E

K

R

E

K

K

S
x
A

L
|
L

V

V

K

M

G

Q

E

P

I

L

N

D

V

T

S

Q

M

R

E

D

K

A

C

Q

I

V

Y

P

C

N

G

L

E

E

C

Y

A

A

M

R

C

I

P

P

A

V

S

K

A

S

I

-

E

E

I

V

S

L

W

P

R

R

D

D

binding iron/sulfur cluster: P681 (vs. gap), C688 (= C61), I689 (≠ V62), C691 (= C64), N692 (≠ G65), C694 (= C67), C698 (= C71), P699 (≠ I72), A702 (= A75), I703 (= I76), C744 (= C105), G746 (≠ L107), C747 (= C108), G748 (= G109), N749 (= N110), C750 (= C111), C754 (= C115), A760 (≠ S131), L761 (= L132)

Sites not aligning to the query:

active site: 30, 63, 113, 995
binding calcium ion: 982, 984, 1056, 1058, 1060
binding magnesium ion: 962, 990, 992
binding pyruvic acid: 30, 113, 996
binding iron/sulfur cluster: 458, 810, 811, 814, 816, 839, 1070, 1071
binding thiamine diphosphate: 28, 29, 63, 87, 816, 837, 838, 839, 868, 961, 962, 963, 992, 993, 994, 995, 996

1kekA Crystal structure of the free radical intermediate of pyruvate:ferredoxin oxidoreductase (see paper)
26% identity, 40% coverage: 32:165/332 of query aligns to 654:793/1231 of 1kekA

query
sites
1kekA

P

P

V

V

N

S

A

A

I

F

D

E

R

A

A

D

P

G

L

R

L

F

P

P

I

L

A

G

R

T

G

S

L

Q

I

F

K

E

M

K

N

R

R

G

V

V

S

A

F

I

N

N

-

V

-
x
P

-

Q

-
x
W

-

V

K

P

E

E

K

N

C
|
C

V
x
I

L
x
Q

C
|
C

G
x
N

L

Q

C
|
C

A

A

S

F

V

V

C
|
C

I
x
P

F

H

G

S

A

A

I
|
I

-

L

-

P

-

V

-

L

-

A

-

K

-

E

-

L

-

V

-

G

-

A

-

P

-

A

-

N

-

F

-

T

D

A

L

L

Q

E

K

A

D

K

G

G

K

K

S

E

I

L

R

K

G

G

A

-

D

-

E

-

Y

-

P

-

F

-

W

Y

D

K

F

F

K

R

L
x
I

E

Q

I

I

D

N

D

T

E

L

K

D

C
|
C

F
x
M

L
x
G

C
|
C

G
|
G

N

N

C
|
C

A

A

D

D

A

I

C
|
C

P
|
P

R

-

N

-

A

-

L

-

T

-

I

-

R

-

D

-

L

-

P

P

E

K

R

E

K

K

S
x
A

L
|
L

V

V

K

M

G

Q

E

P

I

L

N

D

V

T

S

Q

M

R

E

D

K

A

C

Q

I

V

Y

P

C

N

G

L

E

E

C

Y

A

A

M

R

C

I

P

P

A

V

S

K

A

S

I

-

E

E

I

V

S

L

W

P

R

R

D

D

binding iron/sulfur cluster: P681 (vs. gap), W683 (vs. gap), C688 (= C61), I689 (≠ V62), Q690 (≠ L63), C691 (= C64), N692 (≠ G65), C694 (= C67), C698 (= C71), P699 (≠ I72), I703 (= I76), I737 (≠ L98), C744 (= C105), M745 (≠ F106), G746 (≠ L107), C747 (= C108), G748 (= G109), C750 (= C111), C754 (= C115), P755 (= P116), A760 (≠ S131), L761 (= L132)

Sites not aligning to the query:

active site: 30, 63, 113, 995
binding calcium ion: 982, 984, 1055, 1056, 1058, 1062
binding 2-acetyl-thiamine diphosphate: 29, 63, 87, 113, 816, 837, 838, 839, 868, 961, 962, 963, 992, 993, 994, 995, 996
binding magnesium ion: 962, 990, 992
binding iron/sulfur cluster: 458, 810, 811, 814, 816, 839, 1070, 1071

1b0pA Crystal structure of pyruvate-ferredoxin oxidoreductase from desulfovibrio africanus (see paper)
26% identity, 40% coverage: 32:165/332 of query aligns to 654:793/1231 of 1b0pA

query
sites
1b0pA

P

P

V

V

N

S

A

A

I

F

D

E

R

A

A

D

P

G

L

R

L

F

P

P

I

L

A

G

R

T

G

S

L

Q

I

F

K

E

M

K

N

R

R

G

V

V

S

A

F

I

N

N

-

V

-
x
P

-

Q

-
x
W

-

V

K

P

E

E

K

N

C
|
C

V
x
I

L
x
Q

C
|
C

G
x
N

L

Q

C
|
C

A

A

S

F

V

V

C
|
C

I
x
P

F

H

G

S

A
|
A

I
|
I

-

L

-

P

-

V

-

L

-

A

-

K

-

E

-

L

-

V

-

G

-

A

-

P

-

A

-

N

-

F

-

T

D

A

L

L

Q

E

K

A

D

K

G

G

K

K

S

E

I

L

R

K

G

G

A

-

D

-

E

-

Y

-

P

-

F

-

W

Y

D

K

F

F

K

R

L
x
I

E

Q

I

I

D

N

D

T

E

L

K

D

C
|
C

F
x
M

L

G

C
|
C

G
|
G

N

N

C
|
C

A

A

D

D

A

I

C
|
C

P
|
P

R

-

N

-

A

-

L

-

T

-

I

-

R

-

D

-

L

-

P

P

E

K

R

E

K

K

S
x
A

L

L

V

V

K

M

G

Q

E

P

I

L

N

D

V

T

S

Q

M

R

E

D

K

A

C

Q

I

V

Y

P

C

N

G

L

E

E

C

Y

A

A

M

R

C

I

P

P

A

V

S

K

A

S

I

-

E

E

I

V

S

L

W

P

R

R

D

D

binding iron/sulfur cluster: P681 (vs. gap), W683 (vs. gap), C688 (= C61), I689 (≠ V62), Q690 (≠ L63), C691 (= C64), N692 (≠ G65), C694 (= C67), C698 (= C71), P699 (≠ I72), A702 (= A75), I703 (= I76), I737 (≠ L98), C744 (= C105), M745 (≠ F106), C747 (= C108), G748 (= G109), C750 (= C111), C754 (= C115), P755 (= P116), A760 (≠ S131)

Sites not aligning to the query:

active site: 30, 63, 113, 995
binding calcium ion: 982, 984, 1055, 1056, 1058, 1060
binding magnesium ion: 962, 990, 992
binding iron/sulfur cluster: 458, 811, 814, 816, 839, 994, 1070, 1071
binding thiamine diphosphate: 28, 29, 63, 816, 837, 838, 839, 868, 961, 962, 963, 992, 993, 994, 995, 996

P94692 Pyruvate:ferredoxin oxidoreductase; PFOR; POR; Pyruvate synthase; EC 1.2.7.1 from Desulfocurvibacter africanus (Desulfovibrio africanus) (see 3 papers)
26% identity, 40% coverage: 32:165/332 of query aligns to 655:794/1232 of P94692

query
sites
P94692

P

P

V

V

N

S

A

A

I

F

D

E

R

A

A

D

P

G

L

R

L

F

P

P

I

L

A

G

R

T

G

S

L

Q

I

F

K

E

M

K

N

R

R

G

V

V

S

A

F

I

N

N

-

V

-

P

-

Q

-

W

-

V

K

P

E

E

K

N

C
|
C

V

I

L

Q

C
|
C

G

N

L

Q

C
|
C

A

A

S

F

V

V

C
|
C

I

P

F

H

G

S

A

A

I

I

-

L

-

P

-

V

-

L

-

A

-

K

-

E

-

L

-

V

-

G

-

A

-

P

-

A

-

N

-

F

-

T

D

A

L

L

Q

E

K

A

D

K

G

G

K

K

S

E

I

L

R

K

G

G

A

-

D

-

E

-

Y

-

P

-

F

-

W

Y

D

K

F

F

K

R

L

I

E

Q

I

I

D

N

D

T

E

L

K

D

C
|
C

F

M

L

G

C
|
C

G

G

N

N

C
|
C

A

A

D

D

A

I

C
|
C

P

P

R

-

N

-

A

-

L

-

T

-

I

-

R

-

D

-

L

-

P

P

E

K

R

E

K

K

S

A

L

L

V

V

K

M

G

Q

E

P

I

L

N

D

V

T

S

Q

M

R

E

D

K

A

C

Q

I

V

Y

P

C

N

G

L

E

E

C

Y

A

A

M

R

C

I

P

P

A

V

S

K

A

S

I

-

E

E

I

V

S

L

W

P

R

R

D

D

C689 (= C61) binding
C692 (= C64) binding
C695 (= C67) binding
C699 (= C71) binding
C745 (= C105) binding
C748 (= C108) binding
C751 (= C111) binding
C755 (= C115) binding

Sites not aligning to the query:

31 binding
64 binding
114 binding
812 binding
815 binding
817 binding
840 binding ; binding
962:965 binding
963 binding
983 binding
985 binding
991 binding
991:996 binding
993 binding
1056 binding
1059 binding
1061 binding
1063 binding
1071 binding

5gupH structure of mammalian respiratory supercomplex I1III2IV1 (see paper)
28% identity, 39% coverage: 19:149/332 of query aligns to 29:159/176 of 5gupH

query
sites
5gupH

D

E

L
|
L

C

V

T

R

G

G

C

L

G

G

-

M

-

T

-

L

-

S

-

Y

L

L

C

F

S

R

E

E

T

P

C

A

P

T

V

I

N

N

-

Y

A

P

I

F

D

E

R

K

A

G

P

P

L

L

L

S

P

P

I

R

A

F

R

R

G

G

L

E

I
x
H

K

A

M

L

N

R

R

R

V

Y

S

P

F

S

N

G

K

E

E

E

K

R

C
|
C

V
x
I

L
x
A

C
|
C

G
x
K

L

L

C
|
C

A

E

S

A

V

V

C
|
C

I

P

F

A

G

Q

A

A

I

I

D

T

L

I

Q

E

K

A

D

E

G

P

K

R

S

A

I

-

R

D

G

G

A

S

D

R

E

R

Y

T

P

T

F
x
R

W

Y

D

D

F

-

K

-

L

-

E

-

I

I

D

D

D

M

E

T

K

K

C
|
C

F
x
I

L
x
Y

C
|
C

G
|
G

N
x
F

C
|
C

A

Q

D

E

A

A

C
|
C

P
|
P

R

V

N

D

A
|
A

L
x
I

L

V

T

E

I

G

R

P

D

N

L

F

P

-

E

E

R

F

K

S

S

T

L

E

V

T

K

H

G

E

E

E

I

L

N

L

V

Y

S
x
N

M

K

E

E

K

K

C

L

I

L

Y

N

C

N

G

G

E

D

binding (9r,11s)-9-({[(1s)-1-hydroxyhexadecyl]oxy}methyl)-2,2-dimethyl-5,7,10-trioxa-2lambda~5~-aza-6lambda~5~-phosphaoctacosane-6,6,11-triol: L30 (= L20)
binding iron/sulfur cluster: H65 (≠ I49), C77 (= C61), I78 (≠ V62), A79 (≠ L63), C80 (= C64), K81 (≠ G65), C83 (= C67), C87 (= C71), C116 (= C105), I117 (≠ F106), Y118 (≠ L107), C119 (= C108), G120 (= G109), F121 (≠ N110), C122 (= C111), C126 (= C115), P127 (= P116), A130 (= A119), I131 (≠ L120)
binding : R108 (≠ F93), N150 (≠ S140)

Sites not aligning to the query:

binding (9r,11s)-9-({[(1s)-1-hydroxyhexadecyl]oxy}methyl)-2,2-dimethyl-5,7,10-trioxa-2lambda~5~-aza-6lambda~5~-phosphaoctacosane-6,6,11-triol: 24, 27

8iufS8 ndufv2 (see paper)
47% identity, 17% coverage: 142:199/332 of query aligns to 73:129/182 of 8iufS8

query
sites
8iufS8

E

E

K

R

C
|
C

I
|
I

Y
x
T

C
|
C

G
x
Q

E

L

C
|
C

A

E

A

V

M

T

C
|
C

P
|
P

A

A

S

Q

A

A

I
|
I

E

A

I

I

S

D

W

G

-

V

R

E

D

D

P

D

D

D

S

G

S

S

N

R

M

M

A

A

I

-

A

-

D

S

G

R

I

F

R

D

V

L

D

D

E

M

D

H

K

K

C
|
C

L
x
I

Y
|
Y

C
|
C

G
|
G

I

L

C
|
C

K

Q

R

E

I

A

C
|
C

P

P

V

V

G

D

A

A

I
|
I

binding iron/sulfur cluster: C75 (= C144), I76 (= I145), T77 (≠ Y146), C78 (= C147), Q79 (≠ G148), C81 (= C150), C85 (= C154), P86 (= P155), I90 (= I159), C114 (= C184), I115 (≠ L185), Y116 (= Y186), C117 (= C187), G118 (= G188), C120 (= C190), C124 (= C194), I129 (= I199)

Sites not aligning to the query:

binding iron/sulfur cluster: 63, 131

7p63I Complex i from e. Coli, ddm/lmng-purified, under turnover at ph 6, closed state (see paper)
37% identity, 26% coverage: 41:126/332 of query aligns to 40:120/180 of 7p63I

query
sites
7p63I

L

L

L

P

P

P

I

R

A

Y

R

R

G

G

L

R

I
|
I

K

V

M

L

N

T

R

R

V

D

S

P

F

D

N

G

K

E

E

E

K

R

C
|
C

V
|
V

L
x
A

C
|
C

G
x
N

L

L

C
|
C

A

A

S

V

V

A

C
|
C

I

P

F

V

G

G

A
x
C

I
|
I

D

S

L

L

Q

Q

K

K

-

A

D

E

G

T

K

K

S

D

I

G

R

R

G

W

A

Y

D

P

E

E

Y

F

P

-

F

-

W

-

D

-

F

-

K

-

L
x
F

E

R

I

I

D

N

D

F

E

S

K

R

C
|
C

F
x
I

L
x
F

C
|
C

G
|
G

N
x
L

C
|
C

A

E

D

E

A

A

C
|
C

P
|
P

R
x
T

N

T

A

A

L
x
I

L

Q

T

L

I

T

R

P

D

D

L

F

binding iron/sulfur cluster: I48 (= I49), C60 (= C61), V61 (= V62), A62 (≠ L63), C63 (= C64), N64 (≠ G65), C66 (= C67), C70 (= C71), C74 (≠ A75), I75 (= I76), F92 (≠ L98), C99 (= C105), I100 (≠ F106), F101 (≠ L107), C102 (= C108), G103 (= G109), L104 (≠ N110), C105 (= C111), C109 (= C115), P110 (= P116), T111 (≠ R117), I114 (≠ L120)

Q56224 NADH-quinone oxidoreductase subunit 9; NADH dehydrogenase I subunit 9; NDH-1 subunit 9; EC 7.1.1.- from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
37% identity, 24% coverage: 140:218/332 of query aligns to 49:132/182 of Q56224

query
sites
Q56224

S

G

M

L

E

E

K

K

C
|
C

I

I

Y

G

C
|
C

G
x
S

E

L

C
|
C

A

A

M

A

C
|
C

P

P

A

A

S

Y

A

A

I

I

-

Y

-

V

E

E

I

P

S

A

W

E

R

N

D

D

P

P

D

E

-

N

-

P

-

V

S

S

S

A

N

G

M

E

A

R

I

Y

A

A

D

K

G

V

I

Y

R

E

V

I

D

N

E

M

D

L

K

R

C
|
C

L
x
I

Y

F

C
|
C

G

G

I

L

C
|
C

K

E

R

E

I

A

C
|
C

P

P

V

T

G

G

A

A

I

I

R

V

M

L

S

G

C

Y

L

D

T

F

C

E

M

M

Y

A

N

D

E

Y

E

E

L

Y

K

S

A

D

T

L

V

V

E

Y

G

G

C53 (= C144) binding
C56 (= C147) binding
S57 (≠ G148) binding
C59 (= C150) binding
C63 (= C154) binding
C98 (= C184) binding
I99 (≠ L185) binding
C101 (= C187) binding
C104 (= C190) binding
C108 (= C194) binding

4hea9 Crystal structure of the entire respiratory complex i from thermus thermophilus (see paper)
37% identity, 24% coverage: 140:218/332 of query aligns to 48:131/180 of 4hea9

query
sites
4hea9

S

G

M

L

E

E

K

K

C
|
C

I

I

Y
x
G

C
|
C

G
x
S

E
x
L

C
|
C

A

A

M

A

C
|
C

P
|
P

A

A

S

Y

A

A

I
|
I

-

Y

-

V

E

E

I

P

S

A

W

E

R

N

D

D

P

P

D

E

-

N

-

P

-

V

S

S

S

A

N

G

M

E

A

R

I

Y

A

A

D

K

G

V

I
x
Y

R

E

V

I

D

N

E

M

D

L

K

R

C
|
C

L
x
I

Y
x
F

C
|
C

G
|
G

I
x
L

C
|
C

K

E

R

E

I

A

C
|
C

P
|
P

V
x
T

G

G

A
|
A

I
|
I

R

V

M

L

S

G

C

Y

L

D

T

F

C

E

M

M

Y

A

N

D

E

Y

E

E

L

Y

K

S

A

D

T

L

V

V

E

Y

G

G

binding iron/sulfur cluster: C52 (= C144), G54 (≠ Y146), C55 (= C147), S56 (≠ G148), L57 (≠ E149), C58 (= C150), C62 (= C154), P63 (= P155), I67 (= I159), Y90 (≠ I177), C97 (= C184), I98 (≠ L185), F99 (≠ Y186), C100 (= C187), G101 (= G188), L102 (≠ I189), C103 (= C190), C107 (= C194), P108 (= P195), T109 (≠ V196), A111 (= A198), I112 (= I199)

Sites not aligning to the query:

binding iron/sulfur cluster: 40

7ak5I Cryo-em structure of respiratory complex i in the deactive state from mus musculus at 3.2 a (see paper)
30% identity, 31% coverage: 19:121/332 of query aligns to 31:134/178 of 7ak5I

query
sites
7ak5I

D

E

L

L

C

I

T

R

G

G

C

L

G

G

-

M

-

T

-

L

-

S

-

Y

L

L

C

F

S

R

E

E

T

P

C

A

P

T

V

I

N

N

-

Y

A

P

I

F

D

E

R

K

A

G

P

P

L

L

L

S

P

P

I

R

A

F

R

R

G

G

L

E

I
x
H

K

A

M

L

N

R

R

R

V

Y

S

P

F

S

N

G

K

E

E

E

K

R

C
|
C

V
x
I

L
x
A

C
|
C

G
x
K

L

L

C
|
C

A

E

S

A

V

I

C
|
C

I

P

F

A

G

Q

A

A

I

I

D

T

L

I

Q

E

K

A

D

E

G

P

K

R

S

A

I

-

R

D

G

G

A

S

D

R

E

R

Y

T

P

T

F

R

W

Y

D

D

F

-

K

-

L

-

E

-

I

I

D

D

D

M

E

T

K

K

C
|
C

F
x
I

L
x
Y

C
|
C

G
|
G

N

F

C
|
C

A

Q

D

E

A

A

C
|
C

P

P

R

V

N

D

A

A

L
x
I

L

V

binding iron/sulfur cluster: H67 (≠ I49), C79 (= C61), I80 (≠ V62), A81 (≠ L63), C82 (= C64), K83 (≠ G65), C85 (= C67), C89 (= C71), C118 (= C105), I119 (≠ F106), Y120 (≠ L107), C121 (= C108), G122 (= G109), C124 (= C111), C128 (= C115), I133 (≠ L120)

Sites not aligning to the query:

binding iron/sulfur cluster: 135

8b6fAL ndutt15 (see paper)
31% identity, 26% coverage: 36:121/332 of query aligns to 93:173/218 of 8b6fAL

query
sites
8b6fAL

I

F

D

E

R

K

A

G

P

P

L

L

L

S

P

P

I

L

A

F

R

R

G

G

L

E

I
x
H

K

A

M

L

N

R

R

R

V

Y

S

P

F

T

N

G

K

E

E

E

K

R

C
|
C

V
x
I

L

A

C
|
C

G
x
K

L

L

C
|
C

A

Q

S

S

V

A

C
|
C

I

P

F

A

G

R

A

A

I
|
I

D

T

L

I

Q

E

K

T

D

E

G

P

K

R

-

P

-

D

-

G

S

S

I

R

R

R

G

T

A

V

D

-

E

-

Y

-

P

-

F

-

W

-

D

-

F

-

K

R

L
x
Y

E

D

I

I

D

D

D

M

E

T

K

K

C
|
C

F
x
I

L
x
Y

C
|
C

G
|
G

N

F

C
|
C

A

Q

D

E

A

A

C
|
C

P
|
P

R

V

N

D

A
|
A

L
x
I

L

V

binding iron/sulfur cluster: H106 (≠ I49), C118 (= C61), I119 (≠ V62), C121 (= C64), K122 (≠ G65), C124 (= C67), C128 (= C71), I133 (= I76), Y150 (≠ L98), C157 (= C105), I158 (≠ F106), Y159 (≠ L107), C160 (= C108), G161 (= G109), C163 (= C111), C167 (= C115), P168 (= P116), A171 (= A119), I172 (≠ L120)

Sites not aligning to the query:

binding iron/sulfur cluster: 174

8b9zI Drosophila melanogaster complex i in the active state (dm1) (see paper)
31% identity, 26% coverage: 36:121/332 of query aligns to 62:142/186 of 8b9zI

query
sites
8b9zI

I

F

D

E

R

K

A

G

P

P

L

L

L

S

P

P

I

R

A

F

R

R

G

G

L

E

I
x
H

K

A

M

L

N

R

R

R

V

Y

S

P

F

S

N

G

K

E

E

E

K

R

C
|
C

V
x
I

L
x
A

C
|
C

G
x
K

L

L

C
|
C

A

E

S

A

V

I

C
|
C

I
x
P

F

A

G

Q

A

A

I

I

D

T

L

I

Q

E

K

A

D

E

G

E

K

R

S

A

I

-

R

D

G

G

A

S

D

R

E

R

Y

T

P

T

F

R

W
x
Y

D

D

F

-

K

-

L

-

E

-

I

I

D

D

D

M

E

T

K

K

C
|
C

F
x
I

L
x
Y

C
|
C

G
|
G

N
x
F

C
|
C

A

Q

D

E

A

A

C
|
C

P

P

R

V

N

D

A
|
A

L
x
I

L

V

binding iron/sulfur cluster: H75 (≠ I49), C87 (= C61), I88 (≠ V62), A89 (≠ L63), C90 (= C64), K91 (≠ G65), C93 (= C67), C97 (= C71), P98 (≠ I72), Y119 (≠ W94), C126 (= C105), I127 (≠ F106), Y128 (≠ L107), C129 (= C108), G130 (= G109), F131 (≠ N110), C132 (= C111), C136 (= C115), A140 (= A119), I141 (≠ L120)

Sites not aligning to the query:

binding iron/sulfur cluster: 143

Query Sequence

>WP_010876559.1 NCBI__GCF_000008645.1:WP_010876559.1
MLVNERMGSERRTLNYNPDLCTGCGLCSETCPVNAIDRAPLLPIARGLIKMNRVSFNKEK
CVLCGLCASVCIFGAIDLQKDGKSIRGADEYPFWDFKLEIDDEKCFLCGNCADACPRNAL
LTIRDLPERKSLVKGEINVSMEKCIYCGECAAMCPASAIEISWRDPDSSNMAIADGIRVD
EDKCLYCGICKRICPVGAIRMSCLTCMYNEELKATVEGAVITIDERCAHCGWCMEICPAN
AITVKKPIRGTISQADERCRGESCHACVDVCPCNAISIINGTARIDEKFCVFCGACSSVC
PDGLLSIERSELRIRNLKSVAWEHIIKTALQK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory