SitesBLAST – Find functional sites

 

SitesBLAST

Comparing WP_010876999.1 NCBI__GCF_000008645.1:WP_010876999.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 11 hits to proteins with known functional sites (download)

Q58667 Methanogen homoaconitase small subunit; HACN; Homoaconitate hydratase; EC 4.2.1.114 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
54% identity, 97% coverage: 1:157/162 of query aligns to 3:159/170 of Q58667

query
sites
Q58667
M
 
I
K
 
K
G
 
G
K
 
R
V
 
A
W
 
H
K
 
K
F
 
F
P
 
G
D
 
D
D
 
D
V
 
V
D
 
D
T
 
T
D
 
D
I
 
A
I
 
I
I
 
I
P
 
P
G
 
G
R
 
P
Y
 
Y
L
 
L
V
x
R
M
x
T
R
 
T
D
 
D
P
 
P
E
 
Y
K
 
E
L
 
L
R
 
A
E
 
S
H
 
H
V
 
C
M
 
M
E
 
A
G
 
G
L
 
I
D
 
D
P
 
E
E
 
N
F
 
F
P
 
P
S
 
K
K
 
K
V
 
V
K
 
K
P
 
E
G
 
G
D
 
D
F
 
V
I
 
I
V
 
V
A
 
A
G
 
G
K
 
E
N
 
N
F
 
F
G
 
G
C
 
C
G
 
G
S
 
S
S
 
S
R
 
R
E
 
E
H
 
Q
A
 
A
P
 
V
L
 
I
A
 
A
L
 
I
K
 
K
G
 
Y
A
 
C
G
 
G
I
 
I
A
 
K
A
 
A
V
 
V
I
 
I
A
 
A
E
 
K
S
 
S
F
 
F
A
 
A
R
 
R
I
 
I
F
 
F
Y
 
Y
R
 
R
N
 
N
A
 
A
I
 
I
N
 
N
V
 
V
G
 
G
-
 
L
I
 
I
P
 
P
L
 
I
L
 
I
E
 
A
A
 
N
P
 
-
G
 
-
I
 
-
T
 
T
E
 
D
K
 
E
L
 
I
N
 
K
E
 
D
G
 
G
D
 
D
E
 
I
I
 
V
E
 
E
V
 
I
D
 
D
L
 
L
D
 
D
R
 
K
G
 
E
-
 
E
-
 
I
V
 
V
I
 
I
I
 
T
R
 
N
G
 
K
D
 
N
D
 
K
E
 
T
F
 
I
P
 
K
F
 
C
K
 
E
K
 
T
L
 
P
P
 
K
D
 
G
F
 
L
M
 
E
V
 
R
E
 
E
I
 
I
L
 
L
E
 
A
K
 
A
G
 
G
G
 
G
L
 
L
I
 
V
P
 
N
Y
 
Y
L
 
L
K
 
K
K
 
K
K
 
R

2pkpA Crystal structure of 3-isopropylmalate dehydratase (leud)from methhanocaldococcus jannaschii dsm2661 (mj1271) (see paper)
54% identity, 97% coverage: 1:157/162 of query aligns to 3:159/167 of 2pkpA

query
sites
2pkpA
M
 
I
K
 
K
G
 
G
K
 
R
V
 
A
W
 
H
K
 
K
F
 
F
P
 
G
D
 
D
D
|
D
V
 
V
D
 
D
T
 
T
D
 
D
I
 
A
I
 
I
I
 
I
P
 
P
G
 
G
R
 
P
Y
 
Y
L
 
L
V
 
R
M
 
T
R
 
T
D
 
D
P
 
P
E
 
Y
K
 
E
L
 
L
R
 
A
E
 
S
H
 
H
V
 
C
M
 
M
E
 
A
G
 
G
L
 
I
D
 
D
P
 
E
E
 
N
F
 
F
P
 
P
S
 
K
K
 
K
V
 
V
K
 
K
P
 
E
G
 
G
D
 
D
F
 
V
I
 
I
V
 
V
A
 
A
G
 
G
K
 
E
N
 
N
F
 
F
G
 
G
C
|
C
G
 
G
S
 
S
S
 
S
R
 
R
E
 
E
H
 
Q
A
 
A
P
 
V
L
 
I
A
 
A
L
 
I
K
 
K
G
 
Y
A
 
C
G
 
G
I
 
I
A
 
K
A
 
A
V
 
V
I
 
I
A
 
A
E
 
K
S
 
S
F
 
F
A
 
A
R
 
R
I
 
I
F
 
F
Y
 
Y
R
 
R
N
 
N
A
 
A
I
 
I
N
 
N
V
 
V
G
 
G
-
 
L
I
 
I
P
 
P
L
 
I
L
 
I
E
 
A
A
 
N
P
 
-
G
 
-
I
 
-
T
 
T
E
 
D
K
 
E
L
 
I
N
 
K
E
 
D
G
 
G
D
 
D
E
 
I
I
 
V
E
 
E
V
 
I
D
 
D
L
 
L
D
 
D
R
 
K
G
 
E
-
 
E
-
 
I
V
 
V
I
 
I
I
 
T
R
 
N
G
 
K
D
 
N
D
 
K
E
 
T
F
 
I
P
 
K
F
 
C
K
 
E
K
 
T
L
 
P
P
 
K
D
 
G
F
 
L
M
 
E
V
 
R
E
 
E
I
 
I
L
 
L
E
 
A
K
 
A
G
 
G
G
 
G
L
 
L
I
 
V
P
 
N
Y
 
Y
L
 
L
K
 
K
K
 
K
K
 
R

O14289 3-isopropylmalate dehydratase; Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase; EC 4.2.1.33 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
35% identity, 79% coverage: 11:138/162 of query aligns to 554:697/758 of O14289

query
sites
O14289
D
 
N
V
 
V
D
 
D
T
 
T
D
 
D
I
 
K
I
 
I
I
 
I
P
 
P
G
 
K
R
 
Q
Y
 
F
L
 
L
V
 
K
M
 
T
R
 
I
D
 
K
P
 
R
E
 
T
K
 
G
L
 
L
R
 
G
E
 
Q
H
 
F
V
 
A
M
 
F
E
 
Y
G
 
E
L
 
I
D
 
R
-
 
Y
-
 
D
-
 
A
-
 
D
-
 
G
-
 
K
-
 
E
-
 
I
P
 
P
E
 
D
F
 
F
P
 
V
S
 
L
K
 
N
V
 
R
K
 
E
P
 
P
-
 
Y
-
 
R
-
 
H
G
 
A
D
 
T
F
 
V
I
 
L
V
 
V
A
 
A
G
 
H
K
 
D
N
 
N
F
 
F
G
 
G
C
 
C
G
 
G
S
 
S
S
 
S
R
 
R
E
 
E
H
 
H
A
 
A
P
 
P
L
 
W
A
 
A
L
 
L
K
 
N
G
 
D
A
 
F
G
 
G
I
 
I
A
 
R
A
 
V
V
 
I
I
 
I
A
 
A
E
 
P
S
 
S
F
 
F
A
 
A
R
 
D
I
 
I
F
 
F
Y
 
F
R
 
N
N
 
N
A
 
C
I
 
F
N
 
K
V
 
N
G
 
G
I
 
-
P
 
-
L
 
M
L
 
L
E
 
P
A
 
I
P
 
P
G
 
T
I
 
P
T
 
I
E
 
E
K
 
Q
L
 
V
N
 
N
E
 
D
-
 
M
-
 
M
-
 
K
-
 
A
-
 
A
-
 
E
-
 
N
G
 
Q
D
 
V
E
 
K
I
 
F
E
 
S
V
 
V
D
 
D
L
 
L
D
 
V
R
 
N
G
 
Q
V
 
T
I
 
I
I
 
T
R
 
Y
G
 
G
D
 
D
D
 
K
E
 
Q
F
 
V
P
 
K
F
 
F
K
 
D
K
 
V
L
 
E
P
 
P

Sites not aligning to the query:

P9WK95 3-isopropylmalate dehydratase small subunit; Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase; EC 4.2.1.33 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
36% identity, 77% coverage: 11:135/162 of query aligns to 18:154/198 of P9WK95

query
sites
P9WK95
D
 
N
V
 
V
D
 
D
T
 
T
D
 
D
I
 
Q
I
 
I
I
 
I
P
 
P
G
 
A
R
 
V
Y
 
F
L
 
L
V
 
K
M
 
R
R
 
V
D
 
T
P
 
R
E
 
T
K
 
G
L
 
F
R
 
E
E
 
D
H
 
G
V
 
L
M
 
F
E
 
A
G
 
G
L
 
W
-
 
R
-
 
S
D
 
D
P
 
P
E
 
A
F
 
F
P
 
V
S
 
L
K
 
N
V
 
L
K
 
S
P
 
P
-
 
F
-
 
D
-
 
R
G
 
G
D
 
S
F
 
V
I
 
L
V
 
V
A
 
A
G
 
G
K
 
P
N
 
D
F
 
F
G
 
G
C
 
T
G
 
G
S
 
S
S
 
S
R
 
R
E
 
E
H
 
H
A
 
A
P
 
V
L
 
W
A
 
A
L
 
L
K
 
M
G
 
D
A
 
Y
G
 
G
I
 
F
A
 
R
A
 
V
V
 
V
I
 
I
A
 
S
E
 
S
S
 
R
F
 
F
A
 
G
R
 
D
I
 
I
F
 
F
Y
 
R
R
 
G
N
 
N
A
 
A
I
 
G
N
 
K
V
 
A
G
 
G
I
 
L
P
 
L
L
 
A
L
 
A
E
 
E
A
 
V
P
 
A
G
 
Q
-
 
D
-
 
D
-
 
V
-
 
E
I
 
L
T
 
L
E
 
W
K
 
K
L
 
L
N
 
I
E
 
E
-
 
Q
-
 
S
-
 
P
G
 
G
D
 
L
E
 
E
I
 
I
E
 
T
V
 
A
D
 
N
L
 
L
D
 
Q
R
 
D
G
 
R
V
 
I
I
 
I
I
 
T
R
 
A
G
 
A
D
 
T
D
 
V
E
 
V
F
 
L
P
 
P
F
 
F
K
|
K

P09339 Aconitate hydratase A; ACN; Aconitase; Aconitate/2-methylaconitate hydratase; Iron-responsive protein-like; IRP-like; RNA-binding protein; EC 4.2.1.3; EC 4.2.1.- from Bacillus subtilis (strain 168) (see 2 papers)
42% identity, 48% coverage: 48:124/162 of query aligns to 777:857/909 of P09339

query
sites
P09339
K
 
K
P
 
T
G
 
G
D
 
L
F
 
V
I
 
V
V
 
L
A
 
A
G
 
G
K
 
K
N
 
D
F
 
Y
G
 
G
C
 
M
G
 
G
S
 
S
S
 
S
R
 
R
E
 
D
H
 
W
A
 
A
P
 
A
L
 
K
A
 
G
L
 
T
K
 
N
G
 
L
A
 
L
G
 
G
I
 
I
A
 
R
A
 
T
V
 
V
I
 
I
A
 
A
E
 
E
S
 
S
F
 
F
A
 
E
R
 
R
I
 
I
F
 
H
Y
 
R
R
 
S
N
 
N
A
 
L
I
 
V
N
 
F
V
 
M
G
 
G
I
 
V
-
 
L
P
 
P
L
 
L
L
 
Q
E
 
F
A
 
K
P
 
Q
G
 
G
I
 
E
-
 
N
-
 
A
-
 
D
T
 
T
E
 
L
K
 
G
L
 
L
N
 
T
E
 
G
G
 
K
D
 
E
E
 
V
I
 
I
E
 
E
V
 
V
D
 
D
L
 
V
D
 
D
R
 
E
G
 
T
V
 
V

Sites not aligning to the query:

3snpA Crystal structure analysis of iron regulatory protein 1 in complex with ferritin h ire RNA (see paper)
31% identity, 65% coverage: 53:157/162 of query aligns to 729:847/850 of 3snpA

query
sites
3snpA
I
 
V
V
 
L
A
 
A
G
 
G
K
 
K
N
 
E
F
 
Y
G
 
G
C
 
S
G
 
G
S
|
S
S
 
S
R
|
R
E
 
D
H
 
W
A
 
A
P
 
A
L
 
K
A
 
G
L
 
P
K
 
F
G
 
L
A
 
L
G
 
G
I
 
I
A
 
K
A
 
A
V
 
V
I
 
L
A
 
A
E
 
E
S
 
S
F
 
Y
A
 
E
R
 
R
I
 
I
F
 
H
Y
 
R
R
 
S
N
 
N
A
 
L
I
 
V
N
 
G
V
 
M
G
 
G
-
 
V
I
 
I
P
 
P
L
 
L
L
 
E
E
 
Y
A
 
L
P
 
P
G
 
G
-
 
E
-
 
N
-
 
A
-
 
D
-
 
S
-
 
L
-
 
G
-
 
L
-
 
T
-
 
G
-
 
R
-
 
E
-
 
R
-
 
Y
-
 
T
-
 
I
-
 
I
I
 
I
T
 
P
E
 
E
K
 
N
L
 
L
N
 
T
E
 
P
G
 
R
D
 
M
E
 
H
I
 
V
E
 
Q
V
 
V
D
 
K
L
 
L
D
 
D
R
 
T
G
 
G
-
 
K
-
 
T
-
 
F
-
 
Q
V
 
A
I
 
V
I
 
I
R
 
R
G
 
F
D
 
D
D
 
T
E
 
-
F
 
-
P
 
-
F
 
-
K
 
-
K
 
-
L
 
-
P
 
-
D
 
D
F
 
V
M
 
E
V
 
L
E
 
T
I
 
Y
L
 
F
E
 
H
K
 
N
G
 
G
G
 
G
L
 
I
I
 
L
P
 
N
Y
 
Y
L
 
M
K
 
I
K
 
R
K
 
K

Sites not aligning to the query:

P21399 Cytoplasmic aconitate hydratase; Aconitase; Citrate hydro-lyase; Ferritin repressor protein; Iron regulatory protein 1; IRP1; Iron-responsive element-binding protein 1; IRE-BP 1; EC 4.2.1.3 from Homo sapiens (Human) (see 2 papers)
31% identity, 65% coverage: 53:157/162 of query aligns to 768:886/889 of P21399

query
sites
P21399
I
 
V
V
 
L
A
 
A
G
 
G
K
 
K
N
 
E
F
 
Y
G
 
G
C
 
A
G
 
G
S
|
S
S
 
S
R
|
R
E
 
D
H
 
W
A
 
A
P
 
A
L
 
K
A
 
G
L
 
P
K
 
F
G
 
L
A
 
L
G
 
G
I
 
I
A
 
K
A
 
A
V
 
V
I
 
L
A
 
A
E
 
E
S
 
S
F
 
Y
A
 
E
R
 
R
I
 
I
F
 
H
Y
 
R
R
 
S
N
 
N
A
 
L
I
 
V
N
 
G
V
 
M
G
 
G
-
 
V
I
 
I
P
 
P
L
 
L
L
 
E
E
 
Y
A
 
L
P
 
P
G
 
G
-
 
E
-
 
N
-
 
A
-
 
D
-
 
A
-
 
L
-
 
G
-
 
L
-
 
T
-
 
G
-
 
Q
-
 
E
-
 
R
-
 
Y
-
 
T
-
 
I
-
 
I
I
 
I
T
 
P
E
 
E
K
 
N
L
 
L
N
 
K
E
 
P
G
 
Q
D
 
M
E
 
K
I
 
V
E
 
Q
V
 
V
D
 
K
L
 
L
D
 
D
R
 
T
G
 
G
V
 
K
I
 
T
I
 
F
R
 
Q
G
 
A
D
 
V
D
 
M
E
 
R
F
 
F
P
 
D
F
 
-
K
 
-
K
 
-
L
 
-
P
 
T
D
 
D
F
 
V
M
 
E
V
 
L
E
 
T
I
 
Y
L
 
F
E
 
L
K
 
N
G
 
G
G
 
G
L
 
I
I
 
L
P
 
N
Y
 
Y
L
 
M
K
 
I
K
 
R
K
 
K

Sites not aligning to the query:

2b3xA Structure of an orthorhombic crystal form of human cytosolic aconitase (irp1) (see paper)
31% identity, 65% coverage: 53:157/162 of query aligns to 767:885/888 of 2b3xA

query
sites
2b3xA
I
 
V
V
 
L
A
 
A
G
 
G
K
 
K
N
 
E
F
 
Y
G
 
G
C
 
A
G
 
G
S
|
S
S
 
S
R
|
R
E
 
D
H
 
W
A
 
A
P
 
A
L
 
K
A
 
G
L
 
P
K
 
F
G
 
L
A
 
L
G
 
G
I
 
I
A
 
K
A
 
A
V
 
V
I
 
L
A
 
A
E
 
E
S
 
S
F
 
Y
A
 
E
R
 
R
I
 
I
F
 
H
Y
 
R
R
 
S
N
 
N
A
 
L
I
 
V
N
 
G
V
 
M
G
 
G
-
 
V
I
 
I
P
 
P
L
 
L
L
 
E
E
 
Y
A
 
L
P
 
P
G
 
G
-
 
E
-
 
N
-
 
A
-
 
D
-
 
A
-
 
L
-
 
G
-
 
L
-
 
T
-
 
G
-
 
Q
-
 
E
-
 
R
-
 
Y
-
 
T
-
 
I
-
 
I
I
 
I
T
 
P
E
 
E
K
 
N
L
 
L
N
 
K
E
 
P
G
 
Q
D
 
M
E
 
K
I
 
V
E
 
Q
V
 
V
D
 
K
L
 
L
D
 
D
R
 
T
G
 
G
V
 
K
I
 
T
I
 
F
R
 
Q
G
 
A
D
 
V
D
 
M
E
 
R
F
 
F
P
 
D
F
 
-
K
 
-
K
 
-
L
 
-
P
 
T
D
 
D
F
 
V
M
 
E
V
 
L
E
 
T
I
 
Y
L
 
F
E
 
L
K
 
N
G
 
G
G
 
G
L
 
I
I
 
L
P
 
N
Y
 
Y
L
 
M
K
 
I
K
 
R
K
 
K

Sites not aligning to the query:

P19414 Aconitate hydratase, mitochondrial; Aconitase; Citrate hydro-lyase; EC 4.2.1.3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 2 papers)
41% identity, 41% coverage: 53:118/162 of query aligns to 656:723/778 of P19414

query
sites
P19414
I
 
V
V
 
I
A
 
G
G
 
D
K
 
E
N
 
N
F
 
F
G
 
G
C
 
E
G
 
G
S
 
S
S
 
S
R
 
R
E
 
E
H
 
H
A
 
A
P
 
A
L
 
L
A
 
E
L
 
P
K
 
R
G
 
F
A
 
L
G
 
G
I
 
G
A
 
F
A
 
A
V
 
I
I
 
I
A
 
T
E
 
K
S
 
S
F
 
F
A
 
A
R
 
R
I
 
I
F
 
H
Y
 
E
R
 
T
N
 
N
A
 
L
I
 
K
N
 
K
V
 
Q
G
 
G
-
 
L
I
 
L
P
 
P
L
 
L
-
 
N
L
 
F
E
 
K
A
 
N
P
 
P
G
 
A
I
 
D
T
 
Y
E
 
D
K
 
K
L
 
I
N
 
N
E
 
P
G
 
D
D
 
D
E
 
R
I
 
I
E
 
D
V
 
I

Sites not aligning to the query:

D9X0I3 Aconitate hydratase A; ACN; Aconitase; EC 4.2.1.3 from Streptomyces viridochromogenes (strain DSM 40736 / JCM 4977 / BCRC 1201 / Tue 494) (see paper)
44% identity, 32% coverage: 53:104/162 of query aligns to 806:857/931 of D9X0I3

query
sites
D9X0I3
I
 
V
V
 
L
A
 
A
G
 
G
K
 
K
N
 
E
F
 
Y
G
 
G
C
 
S
G
 
G
S
 
S
S
 
S
R
 
R
E
 
D
H
 
W
A
 
A
P
 
A
L
 
K
A
 
G
L
 
T
K
 
A
G
 
L
A
 
L
G
 
G
I
 
V
A
 
K
A
 
A
V
 
V
I
 
I
A
 
A
E
 
E
S
 
S
F
 
Y
A
 
E
R
 
R
I
 
I
F
 
H
Y
 
R
R
 
S
N
 
N
A
 
L
I
 
I
N
 
G
V
 
M
G
 
G
I
 
V
P
 
L
L
 
P
L
 
L
E
 
Q
A
 
F
P
 
P

Sites not aligning to the query:

A0QX20 Aconitate hydratase A; ACN; Aconitase; (2R,3S)-2-methylisocitrate dehydratase; (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; Iron-responsive protein-like; IRP-like; Probable 2-methyl-cis-aconitate hydratase; RNA-binding protein; EC 4.2.1.3; EC 4.2.1.99 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
30% identity, 56% coverage: 15:105/162 of query aligns to 758:864/943 of A0QX20

query
sites
A0QX20
D
 
E
I
 
V
I
 
M
I
 
I
P
 
R
G
 
G
R
 
T
Y
 
F
L
 
A
V
 
N
M
 
I
R
 
R
D
 
L
P
 
R
E
 
N
K
 
Q
L
 
L
R
 
L
E
 
D
H
 
D
V
 
V
M
 
S
E
 
G
G
 
G
L
 
Y
D
 
T
P
 
R
E
 
D
F
 
F
P
 
T
S
 
Q
K
 
P
V
 
G
K
 
G
P
 
P
G
 
Q
D
 
A
F
 
F
I
 
I
-
 
Y
-
 
D
-
 
A
-
 
S
-
 
E
-
 
N
-
 
Y
-
 
K
-
 
K
-
 
A
-
 
G
-
 
I
-
 
P
-
 
L
-
 
V
-
 
V
V
 
L
A
 
G
G
 
G
K
 
K
N
 
E
F
 
Y
G
 
G
C
 
S
G
 
G
S
 
S
S
 
S
R
 
R
E
 
D
H
 
W
A
 
A
P
 
A
L
 
K
A
 
G
L
 
T
K
 
V
G
 
L
A
 
L
G
 
G
I
 
V
A
 
K
A
 
A
V
 
V
I
 
I
A
 
T
E
 
E
S
 
S
F
 
F
A
 
E
R
 
R
I
 
I
F
 
H
Y
 
R
R
 
S
N
 
N
A
 
L
I
 
I
N
 
G
V
 
M
G
 
G
I
 
V
P
 
I
L
 
P
L
 
L
E
 
Q
A
 
F
P
 
P
G
 
A

Sites not aligning to the query:

Query Sequence

>WP_010876999.1 NCBI__GCF_000008645.1:WP_010876999.1
MKGKVWKFPDDVDTDIIIPGRYLVMRDPEKLREHVMEGLDPEFPSKVKPGDFIVAGKNFG
CGSSREHAPLALKGAGIAAVIAESFARIFYRNAINVGIPLLEAPGITEKLNEGDEIEVDL
DRGVIIRGDDEFPFKKLPDFMVEILEKGGLIPYLKKKGDFKG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory