SitesBLAST
Comparing WP_010877267.1 NCBI__GCF_000008645.1:WP_010877267.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5ey5B Lbcats
67% identity, 97% coverage: 5:385/392 of query aligns to 1:382/383 of 5ey5B
- binding pyridoxal-5'-phosphate: H81 (= H84), K82 (= K85), Q109 (= Q112), S185 (= S188), G227 (= G230), G229 (= G232), S230 (= S233), N231 (= N234), E345 (= E347), S371 (= S374), G372 (= G375)
5t6mB Structure of the tryptophan synthase b-subunit from pyroccus furiosus with b-methyltryptophan non-covalently bound (see paper)
64% identity, 98% coverage: 7:389/392 of query aligns to 3:386/386 of 5t6mB
1v8zA X-ray crystal structure of the tryptophan synthase b2 subunit from hyperthermophile, pyrococcus furiosus (see paper)
64% identity, 98% coverage: 7:389/392 of query aligns to 3:386/386 of 1v8zA
- active site: K82 (= K85), E104 (= E107), S371 (= S374)
- binding pyridoxal-5'-phosphate: H81 (= H84), K82 (= K85), Q109 (= Q112), S185 (= S188), G227 (= G230), G228 (= G231), G229 (= G232), S230 (= S233), N231 (= N234), E345 (= E347), S371 (= S374), G372 (= G375)
5dw0A Trpb from pyrococcus furiosus with l-serine bound as the external aldimine (see paper)
64% identity, 98% coverage: 7:389/392 of query aligns to 3:386/388 of 5dw0A
- active site: K82 (= K85), E104 (= E107), S371 (= S374)
- binding [3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-serine: H81 (= H84), K82 (= K85), T105 (= T108), G106 (= G109), A107 (= A110), Q109 (= Q112), H110 (= H113), S185 (= S188), G227 (= G230), G229 (= G232), S230 (= S233), N231 (= N234), G298 (= G300), D300 (= D302), E345 (= E347), S371 (= S374)
6am8B Engineered tryptophan synthase b-subunit from pyrococcus furiosus, pftrpb2b9 with trp bound as e(aex2) (see paper)
64% identity, 96% coverage: 7:384/392 of query aligns to 3:381/385 of 6am8B
- active site: K82 (= K85), E104 (= E107), S371 (= S374)
- binding [3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-l-tryptophane: H81 (= H84), K82 (= K85), E104 (= E107), T105 (= T108), G106 (= G109), A107 (= A110), Q109 (= Q112), H110 (= H113), L161 (= L164), S185 (= S188), V187 (≠ M190), G227 (= G230), G228 (= G231), G229 (= G232), S230 (= S233), N231 (= N234), G298 (= G300), Y301 (= Y303), E345 (= E347), S371 (= S374), G372 (= G375)
- binding tryptophan: P12 (= P16), L169 (≠ M172), S274 (≠ L276), H275 (= H277)
5dw3A Tryptophan synthase beta-subunit from pyrococcus furiosus with product l-tryptophan non-covalently bound in the active site (see paper)
64% identity, 96% coverage: 7:384/392 of query aligns to 3:380/383 of 5dw3A
- active site: K82 (= K85), E104 (= E107), S370 (= S374)
- binding tryptophan: K82 (= K85), E104 (= E107), T105 (= T108), G106 (= G109), A107 (= A110), Q109 (= Q112), H110 (= H113), S185 (= S188), G228 (= G231), Y300 (= Y303)
5ixjD Tryptophan synthase beta-subunit from pyrococcus furiosus with l- threonine non-covalently bound in the active site (see paper)
64% identity, 98% coverage: 7:389/392 of query aligns to 3:384/394 of 5ixjD
5t6mA Structure of the tryptophan synthase b-subunit from pyroccus furiosus with b-methyltryptophan non-covalently bound (see paper)
64% identity, 96% coverage: 7:384/392 of query aligns to 3:379/383 of 5t6mA
7rnpA Engineered tryptophan synthase b-subunit from pyrococcus furiosus, pftrpb2b9_h275e with 4-cl-trp non-covalently bound (see paper)
64% identity, 96% coverage: 7:384/392 of query aligns to 3:381/384 of 7rnpA
5vm5D Engineered tryptophan synthase b-subunit from pyrococcus furiosus, pftrpb2b9, with ser bound (see paper)
64% identity, 96% coverage: 7:384/392 of query aligns to 3:379/383 of 5vm5D
- active site: K82 (= K85), E104 (= E107), S369 (= S374)
- binding 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid: H81 (= H84), K82 (= K85), T105 (= T108), G106 (= G109), A107 (= A110), Q109 (= Q112), H110 (= H113), S185 (= S188), G227 (= G230), G229 (= G232), S230 (= S233), N231 (= N234), G296 (= G300), E343 (= E347), S369 (= S374)
6cutA Engineered holo trpb from pyrococcus furiosus, pftrpb7e6 with (2s,3s)- isopropylserine bound as the external aldimine (see paper)
64% identity, 96% coverage: 7:384/392 of query aligns to 3:381/385 of 6cutA
- binding (2S,3S)-3-hydroxy-2-[(E)-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)amino]-4-methylpentanoic acid (non-preferred name): H81 (= H84), K82 (= K85), T105 (= T108), G106 (= G109), A107 (= A110), Q109 (= Q112), H110 (= H113), S185 (= S188), G227 (= G230), G229 (= G232), S230 (= S233), N231 (= N234), G298 (= G300), E345 (= E347), S371 (= S374)
6cuzA Engineered trpb from pyrococcus furiosus, pftrpb7e6 with (2s,3r)- ethylserine bound as the amino-acrylate (see paper)
64% identity, 96% coverage: 7:384/392 of query aligns to 3:381/383 of 6cuzA
- binding (2E)-2-[(E)-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)amino]pent-2-enoic acid: H81 (= H84), K82 (= K85), T105 (= T108), G106 (= G109), A107 (= A110), Q109 (= Q112), H110 (= H113), S185 (= S188), G227 (= G230), G229 (= G232), S230 (= S233), N231 (= N234), G298 (= G300), E345 (= E347), S371 (= S374)
8vhhA Engineered holo tryptophan synthase (tm9d8 ) Derived from t. Maritima trpb (see paper)
64% identity, 96% coverage: 5:381/392 of query aligns to 2:374/389 of 8vhhA
- binding pyridoxal-5'-phosphate: H81 (= H84), K82 (= K85), Q109 (= Q112), S185 (= S188), G228 (= G231), G229 (= G232), S230 (= S233), N231 (= N234), G297 (= G300), E344 (= E347), S367 (= S374)
8eh1A Engineered tyrosine synthase (tmtyrs1) derived from t. Maritima trpb with ser bound as the amino-acrylate intermediate and complexed with 4-hydroxyquinoline (see paper)
63% identity, 96% coverage: 5:381/392 of query aligns to 1:373/383 of 8eh1A
- binding 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid: H80 (= H84), K81 (= K85), T104 (= T108), G105 (= G109), A106 (= A110), Q108 (= Q112), H109 (= H113), S184 (= S188), G228 (= G232), S229 (= S233), N230 (= N234), G296 (= G300), E343 (= E347), S366 (= S374)
- binding quinolin-4-ol: G103 (≠ E107), L160 (= L164), I164 (= I168), G183 (= G187), S184 (= S188), Y299 (= Y303)
8eh0A Engineered tyrosine synthase (tmtyrs1) derived from t. Maritima trpb with ser bound as the amino-acrylate intermediate and complexed with quinoline n-oxide (see paper)
63% identity, 96% coverage: 5:381/392 of query aligns to 1:373/385 of 8eh0A
- binding 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid: H80 (= H84), K81 (= K85), T104 (= T108), G105 (= G109), A106 (= A110), Q108 (= Q112), H109 (= H113), S184 (= S188), G228 (= G232), S229 (= S233), N230 (= N234), G296 (= G300), E343 (= E347), S366 (= S374), G367 (= G375)
- binding 1-oxo-1lambda~5~-quinoline: L160 (= L164), I164 (= I168), Y180 (= Y184), P182 (≠ I186), G183 (= G187), S184 (= S188), V186 (≠ M190), Y299 (= Y303)
8egzA Engineered tyrosine synthase (tmtyrs1) derived from t. Maritima trpb with ser bound as the amino-acrylate intermediate (see paper)
63% identity, 96% coverage: 5:381/392 of query aligns to 1:373/385 of 8egzA
- binding 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid: H80 (= H84), K81 (= K85), T104 (= T108), G105 (= G109), A106 (= A110), Q108 (= Q112), H109 (= H113), S184 (= S188), S226 (≠ G230), G228 (= G232), S229 (= S233), N230 (= N234), G296 (= G300), E343 (= E347), S366 (= S374)
5kzmB Crystal structure of tryptophan synthase alpha-beta chain complex from francisella tularensis (see paper)
56% identity, 98% coverage: 3:388/392 of query aligns to 4:390/395 of 5kzmB
6u6cB Crystal structure of tryptophan synthase from m. Tuberculosis - aminoacrylate- and gsk2-bound form (see paper)
58% identity, 98% coverage: 5:390/392 of query aligns to 14:403/405 of 6u6cB
- active site: K98 (= K85), E120 (= E107), S387 (= S374)
- binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: H97 (= H84), K98 (= K85), T121 (= T108), G122 (= G109), A123 (= A110), Q125 (= Q112), H126 (= H113), T201 (≠ S188), G243 (= G230), G245 (= G232), S246 (= S233), N247 (= N234), G314 (= G300), E361 (= E347), S387 (= S374)
- binding 1-(2-fluorobenzene-1-carbonyl)-N-methyl-2,3-dihydro-1H-indole-5-sulfonamide: Y26 (≠ F14), F185 (≠ M172), W188 (= W175), Y197 (= Y184), F199 (≠ I186), G204 (= G191), P205 (= P192), H291 (= H277), G292 (= G278)
6usaB Crystal structure of tryptophan synthase from m. Tuberculosis - aminoacrylate- and gsk1-bound form (see paper)
58% identity, 98% coverage: 5:390/392 of query aligns to 13:402/404 of 6usaB
- active site: K97 (= K85), E119 (= E107), S386 (= S374)
- binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: H96 (= H84), K97 (= K85), T120 (= T108), G121 (= G109), A122 (= A110), G123 (= G111), Q124 (= Q112), H125 (= H113), T200 (≠ S188), G242 (= G230), G244 (= G232), S245 (= S233), N246 (= N234), G313 (= G300), E360 (= E347), S386 (= S374)
- binding (3R,4R)-4-[4-(2-Chlorophenyl)piperazin-1-yl]-1,1-dioxothiolan-3-ol: F184 (≠ M172), W187 (= W175), Y196 (= Y184), F198 (≠ I186), G203 (= G191), P204 (= P192), F207 (≠ Y195), H290 (= H277), G291 (= G278)
6dweB Crystal structure of tryptophan synthase from m. Tuberculosis - aminoacrylate- and brd0059-bound form
58% identity, 98% coverage: 5:390/392 of query aligns to 13:402/404 of 6dweB
- active site: K97 (= K85), E119 (= E107), S386 (= S374)
- binding (2R,3S,4R)-3-(2',6'-difluoro-4'-methyl[1,1'-biphenyl]-4-yl)-4-(fluoromethyl)azetidine-2-carbonitrile: F184 (≠ M172), Y196 (= Y184), F198 (≠ I186), P204 (= P192), F207 (≠ Y195), H290 (= H277)
- binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: H96 (= H84), K97 (= K85), T120 (= T108), G121 (= G109), A122 (= A110), G123 (= G111), Q124 (= Q112), H125 (= H113), T200 (≠ S188), G242 (= G230), G244 (= G232), S245 (= S233), N246 (= N234), G313 (= G300), E360 (= E347), S386 (= S374)
Query Sequence
>WP_010877267.1 NCBI__GCF_000008645.1:WP_010877267.1
MIMDGKFGKYGGIFVPELLIPALEELERAFLHYRDDRKFMAELEYHLREYAGKPTGLYYA
RNLSEKLGCRVYLKREDMLHTGAHKINNTIGQALLARYMGKTRIIAETGAGQHGIATAAA
GALFGMDVDIYMGTEDVERQKLNVFRMEVSGAEVIPVDSGSRTLKDAINEAMRDWISNVD
DTHYLIGSTMGPHPYPTMVKHFQSVIGREAREQILEVEGELPDTVIACVGGGSNAIGIFS
AFMDDDVELIGAEGGGEGIESGNHGATLSAGSEGILHGSLSYVLQDGDGQISEAHSVSAG
LDYPGVGPEHAHLMDTGRARYEPVTDTEALRGFRLLSRYEGIMPALESAHAIACLERYAE
KPENRGKTVIVNLSGRGDKDMFMVAGLLGVGV
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory